#METABOLOMICS WORKBENCH stephensondj_20240227_094013 DATATRACK_ID:4676 STUDY_ID:ST003108 ANALYSIS_ID:AN005089 PROJECT_ID:PR001931 VERSION 1 CREATED_ON February 27, 2024, 10:57 am #PROJECT PR:PROJECT_TITLE Complete absence of GLUT1 does not impair human terminal erythroid PR:PROJECT_TITLE differentiation PR:PROJECT_SUMMARY The Glucose transporter 1 (GLUT1) is one of the most abundant proteins within PR:PROJECT_SUMMARY the erythrocyte membrane and is required for glucose and dehydroascorbic acid PR:PROJECT_SUMMARY (Vitamin C precursor) transport. It is widely recognized as a key protein for PR:PROJECT_SUMMARY red cell structure, function, and metabolism. Previous reports highlighted the PR:PROJECT_SUMMARY importance of GLUT1 activity within these uniquely glycolysis-dependent cells, PR:PROJECT_SUMMARY in particular for increasing antioxidant capacity needed to avoid irreversible PR:PROJECT_SUMMARY damage from oxidative stress in humans. However, studies of glucose transporter PR:PROJECT_SUMMARY roles in erythroid cells are complicated by species-specific differences between PR:PROJECT_SUMMARY humans and mice. Here, using CRISPRmediated gene editing of immortalized PR:PROJECT_SUMMARY erythroblasts and adult CD34+ hematopoietic progenitor cells, we generate PR:PROJECT_SUMMARY committed human erythroid cells completely deficient in expression of GLUT1. We PR:PROJECT_SUMMARY show that absence of GLUT1 does not impede human erythroblast proliferation, PR:PROJECT_SUMMARY differentiation, or enucleation. This work demonstrates for the first-time PR:PROJECT_SUMMARY generation of enucleated human reticulocytes lacking GLUT1. The GLUT1-deficient PR:PROJECT_SUMMARY reticulocytes possess no tangible alterations to membrane composition or PR:PROJECT_SUMMARY deformability in reticulocytes. Metabolomic analyses of GLUT1-deficient PR:PROJECT_SUMMARY reticulocytes reveal hallmarks of reduced glucose import, downregulated PR:PROJECT_SUMMARY metabolic processes and upregulated AMPK-signalling, alongside alterations in PR:PROJECT_SUMMARY antioxidant metabolism, resulting in increased osmotic fragility and metabolic PR:PROJECT_SUMMARY shifts indicative of higher oxidant stress. Despite detectable metabolic changes PR:PROJECT_SUMMARY in GLUT1 deficient reticulocytes, the absence of developmental phenotype, PR:PROJECT_SUMMARY detectable proteomic compensation or impaired deformability comprehensively PR:PROJECT_SUMMARY alters our understanding of the role of GLUT1 in red blood cell structure, PR:PROJECT_SUMMARY function and metabolism. It also provides cell biological evidence supporting PR:PROJECT_SUMMARY clinical consensus that reduced GLUT1 expression does not cause anaemia in GLUT1 PR:PROJECT_SUMMARY deficiency syndrome. PR:INSTITUTE University of Colorado PR:LAST_NAME Stephenson PR:FIRST_NAME Daniel PR:ADDRESS Research 1 South L18-1303 12801 E. 17th Ave., Aurora, Colorado, 80045, USA PR:EMAIL daniel.stephenson@cuanschutz.edu PR:PHONE 303-724-3339 #STUDY ST:STUDY_TITLE Complete absence of GLUT1 does not impair human terminal erythroid ST:STUDY_TITLE differentiation ST:STUDY_SUMMARY The Glucose transporter 1 (GLUT1) is one of the most abundant proteins within ST:STUDY_SUMMARY the erythrocyte membrane and is required for glucose and dehydroascorbic acid ST:STUDY_SUMMARY (Vitamin C precursor) transport. It is widely recognized as a key protein for ST:STUDY_SUMMARY red cell structure, function, and metabolism. Previous reports highlighted the ST:STUDY_SUMMARY importance of GLUT1 activity within these uniquely glycolysis-dependent cells, ST:STUDY_SUMMARY in particular for increasing antioxidant capacity needed to avoid irreversible ST:STUDY_SUMMARY damage from oxidative stress in humans. However, studies of glucose transporter ST:STUDY_SUMMARY roles in erythroid cells are complicated by species-specific differences between ST:STUDY_SUMMARY humans and mice. Here, using CRISPRmediated gene editing of immortalized ST:STUDY_SUMMARY erythroblasts and adult CD34+ hematopoietic progenitor cells, we generate ST:STUDY_SUMMARY committed human erythroid cells completely deficient in expression of GLUT1. We ST:STUDY_SUMMARY show that absence of GLUT1 does not impede human erythroblast proliferation, ST:STUDY_SUMMARY differentiation, or enucleation. This work demonstrates for the first-time ST:STUDY_SUMMARY generation of enucleated human reticulocytes lacking GLUT1. The GLUT1-deficient ST:STUDY_SUMMARY reticulocytes possess no tangible alterations to membrane composition or ST:STUDY_SUMMARY deformability in reticulocytes. Metabolomic analyses of GLUT1-deficient ST:STUDY_SUMMARY reticulocytes reveal hallmarks of reduced glucose import, downregulated ST:STUDY_SUMMARY metabolic processes and upregulated AMPK-signalling, alongside alterations in ST:STUDY_SUMMARY antioxidant metabolism, resulting in increased osmotic fragility and metabolic ST:STUDY_SUMMARY shifts indicative of higher oxidant stress. Despite detectable metabolic changes ST:STUDY_SUMMARY in GLUT1 deficient reticulocytes, the absence of developmental phenotype, ST:STUDY_SUMMARY detectable proteomic compensation or impaired deformability comprehensively ST:STUDY_SUMMARY alters our understanding of the role of GLUT1 in red blood cell structure, ST:STUDY_SUMMARY function and metabolism. It also provides cell biological evidence supporting ST:STUDY_SUMMARY clinical consensus that reduced GLUT1 expression does not cause anaemia in GLUT1 ST:STUDY_SUMMARY deficiency syndrome. ST:INSTITUTE University of Colorado ST:LAST_NAME Stephenson ST:FIRST_NAME Daniel ST:ADDRESS Research 1 South L18-1303 12801 E. 17th Ave., Aurora, Colorado, 80045, USA ST:EMAIL daniel.stephenson@cuanschutz.edu ST:PHONE 303-724-3339 #SUBJECT SU:SUBJECT_TYPE Cultured cells SU:SUBJECT_SPECIES Homo sapiens SU:TAXONOMY_ID 9606 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data SUBJECT_SAMPLE_FACTORS Donor 1 DS1-095-001+ Sample source:cultured reticulocytes | Assay:04CF29 RAW_FILE_NAME(Raw File Name)=DS1-095-001+.mzXML; Pellet size=10 Million; Analysis=Metabolomics; Polarity=Positive SUBJECT_SAMPLE_FACTORS Donor 1 DS1-095-002+ Sample source:cultured reticulocytes | Assay:04CF29 RAW_FILE_NAME(Raw File Name)=DS1-095-002+.mzXML; Pellet size=10 Million; Analysis=Metabolomics; Polarity=Positive SUBJECT_SAMPLE_FACTORS Donor 4 DS1-095-003+ Sample source:cultured reticulocytes | Assay:04CF29 RAW_FILE_NAME(Raw File Name)=DS1-095-003+.mzXML; Pellet size=10 Million; Analysis=Metabolomics; Polarity=Positive SUBJECT_SAMPLE_FACTORS Donor 4 DS1-095-004+ Sample source:cultured reticulocytes | Assay:04CF29 RAW_FILE_NAME(Raw File Name)=DS1-095-004+.mzXML; Pellet size=10 Million; Analysis=Metabolomics; Polarity=Positive SUBJECT_SAMPLE_FACTORS Donor 5 DS1-095-005+ Sample source:cultured reticulocytes | Assay:04CF29 RAW_FILE_NAME(Raw File Name)=DS1-095-005+.mzXML; Pellet size=10 Million; Analysis=Metabolomics; Polarity=Positive SUBJECT_SAMPLE_FACTORS Donor 5 DS1-095-006+ Sample source:cultured reticulocytes | Assay:04CF29 RAW_FILE_NAME(Raw File Name)=DS1-095-006+.mzXML; Pellet size=10 Million; Analysis=Metabolomics; Polarity=Positive SUBJECT_SAMPLE_FACTORS Donor A DS1-095-007+ Sample source:cultured reticulocytes | Assay:01CF19 RAW_FILE_NAME(Raw File Name)=DS1-095-007+.mzXML; Pellet size=10 Million; Analysis=Metabolomics; Polarity=Positive SUBJECT_SAMPLE_FACTORS Donor C DS1-095-008+ Sample source:cultured reticulocytes | Assay:01CF19 RAW_FILE_NAME(Raw File Name)=DS1-095-008+.mzXML; Pellet size=10 Million; Analysis=Metabolomics; Polarity=Positive SUBJECT_SAMPLE_FACTORS Donor D DS1-095-009+ Sample source:cultured reticulocytes | Assay:01CF19 RAW_FILE_NAME(Raw File Name)=DS1-095-009+.mzXML; Pellet size=10 Million; Analysis=Metabolomics; Polarity=Positive SUBJECT_SAMPLE_FACTORS Donor A DS1-095-010+ Sample source:cultured reticulocytes | Assay:01CF19 RAW_FILE_NAME(Raw File Name)=DS1-095-010+.mzXML; Pellet size=10 Million; Analysis=Metabolomics; Polarity=Positive SUBJECT_SAMPLE_FACTORS Donor C DS1-095-011+ Sample source:cultured reticulocytes | Assay:01CF19 RAW_FILE_NAME(Raw File Name)=DS1-095-011+.mzXML; Pellet size=10 Million; Analysis=Metabolomics; Polarity=Positive SUBJECT_SAMPLE_FACTORS Donor D DS1-095-012+ Sample source:cultured reticulocytes | Assay:01CF19 RAW_FILE_NAME(Raw File Name)=DS1-095-012+.mzXML; Pellet size=10 Million; Analysis=Metabolomics; Polarity=Positive SUBJECT_SAMPLE_FACTORS Donor A DS1-095-013+ Sample source:cultured reticulocytes | Assay:01CF19 RAW_FILE_NAME(Raw File Name)=DS1-095-013+.mzXML; Pellet size=10 Million; Analysis=Metabolomics; Polarity=Positive SUBJECT_SAMPLE_FACTORS Donor C DS1-095-014+ Sample source:cultured reticulocytes | Assay:01CF19 RAW_FILE_NAME(Raw File Name)=DS1-095-014+.mzXML; Pellet size=10 Million; Analysis=Metabolomics; Polarity=Positive SUBJECT_SAMPLE_FACTORS Donor D DS1-095-015+ Sample source:cultured reticulocytes | Assay:01CF19 RAW_FILE_NAME(Raw File Name)=DS1-095-015+.mzXML; Pellet size=10 Million; Analysis=Metabolomics; Polarity=Positive SUBJECT_SAMPLE_FACTORS Donor A DS1-095-016+ Sample source:Red blood cells | Assay:01CF19 RAW_FILE_NAME(Raw File Name)=DS1-095-016+.mzXML; Pellet size=10 Million; Analysis=Metabolomics; Polarity=Positive SUBJECT_SAMPLE_FACTORS Donor C DS1-095-017+ Sample source:Red blood cells | Assay:01CF19 RAW_FILE_NAME(Raw File Name)=DS1-095-017+.mzXML; Pellet size=10 Million; Analysis=Metabolomics; Polarity=Positive SUBJECT_SAMPLE_FACTORS Donor D DS1-095-018+ Sample source:Red blood cells | Assay:01CF19 RAW_FILE_NAME(Raw File Name)=DS1-095-018+.mzXML; Pellet size=10 Million; Analysis=Metabolomics; Polarity=Positive SUBJECT_SAMPLE_FACTORS Donor 1 DS1-095-001- Sample source:cultured reticulocytes | Assay:04CF29 RAW_FILE_NAME(Raw File Name)=DS1-095-001-.mzXML; Pellet size=10 Million; Analysis=Metabolomics; Polarity=Negative SUBJECT_SAMPLE_FACTORS Donor 1 DS1-095-002- Sample source:cultured reticulocytes | Assay:04CF29 RAW_FILE_NAME(Raw File Name)=DS1-095-002-.mzXML; Pellet size=10 Million; Analysis=Metabolomics; Polarity=Negative SUBJECT_SAMPLE_FACTORS Donor 4 DS1-095-003- Sample source:cultured reticulocytes | Assay:04CF29 RAW_FILE_NAME(Raw File Name)=DS1-095-003-.mzXML; Pellet size=10 Million; Analysis=Metabolomics; Polarity=Negative SUBJECT_SAMPLE_FACTORS Donor 4 DS1-095-004- Sample source:cultured reticulocytes | Assay:04CF29 RAW_FILE_NAME(Raw File Name)=DS1-095-004-.mzXML; Pellet size=10 Million; Analysis=Metabolomics; Polarity=Negative SUBJECT_SAMPLE_FACTORS Donor 5 DS1-095-005- Sample source:cultured reticulocytes | Assay:04CF29 RAW_FILE_NAME(Raw File Name)=DS1-095-005-.mzXML; Pellet size=10 Million; Analysis=Metabolomics; Polarity=Negative SUBJECT_SAMPLE_FACTORS Donor 5 DS1-095-006- Sample source:cultured reticulocytes | Assay:04CF29 RAW_FILE_NAME(Raw File Name)=DS1-095-006-.mzXML; Pellet size=10 Million; Analysis=Metabolomics; Polarity=Negative SUBJECT_SAMPLE_FACTORS Donor A DS1-095-007- Sample source:cultured reticulocytes | Assay:01CF19 RAW_FILE_NAME(Raw File Name)=DS1-095-007-.mzXML; Pellet size=10 Million; Analysis=Metabolomics; Polarity=Negative SUBJECT_SAMPLE_FACTORS Donor C DS1-095-008- Sample source:cultured reticulocytes | Assay:01CF19 RAW_FILE_NAME(Raw File Name)=DS1-095-008-.mzXML; Pellet size=10 Million; Analysis=Metabolomics; Polarity=Negative SUBJECT_SAMPLE_FACTORS Donor D DS1-095-009- Sample source:cultured reticulocytes | Assay:01CF19 RAW_FILE_NAME(Raw File Name)=DS1-095-009-.mzXML; Pellet size=10 Million; Analysis=Metabolomics; Polarity=Negative SUBJECT_SAMPLE_FACTORS Donor A DS1-095-010- Sample source:cultured reticulocytes | Assay:01CF19 RAW_FILE_NAME(Raw File Name)=DS1-095-010-.mzXML; Pellet size=10 Million; Analysis=Metabolomics; Polarity=Negative SUBJECT_SAMPLE_FACTORS Donor C DS1-095-011- Sample source:cultured reticulocytes | Assay:01CF19 RAW_FILE_NAME(Raw File Name)=DS1-095-011-.mzXML; Pellet size=10 Million; Analysis=Metabolomics; Polarity=Negative SUBJECT_SAMPLE_FACTORS Donor D DS1-095-012- Sample source:cultured reticulocytes | Assay:01CF19 RAW_FILE_NAME(Raw File Name)=DS1-095-012-.mzXML; Pellet size=10 Million; Analysis=Metabolomics; Polarity=Negative SUBJECT_SAMPLE_FACTORS Donor A DS1-095-013- Sample source:cultured reticulocytes | Assay:01CF19 RAW_FILE_NAME(Raw File Name)=DS1-095-013-.mzXML; Pellet size=10 Million; Analysis=Metabolomics; Polarity=Negative SUBJECT_SAMPLE_FACTORS Donor C DS1-095-014- Sample source:cultured reticulocytes | Assay:01CF19 RAW_FILE_NAME(Raw File Name)=DS1-095-014-.mzXML; Pellet size=10 Million; Analysis=Metabolomics; Polarity=Negative SUBJECT_SAMPLE_FACTORS Donor D DS1-095-015- Sample source:cultured reticulocytes | Assay:01CF19 RAW_FILE_NAME(Raw File Name)=DS1-095-015-.mzXML; Pellet size=10 Million; Analysis=Metabolomics; Polarity=Negative SUBJECT_SAMPLE_FACTORS Donor A DS1-095-016- Sample source:Red blood cells | Assay:01CF19 RAW_FILE_NAME(Raw File Name)=DS1-095-016-.mzXML; Pellet size=10 Million; Analysis=Metabolomics; Polarity=Negative SUBJECT_SAMPLE_FACTORS Donor C DS1-095-017- Sample source:Red blood cells | Assay:01CF19 RAW_FILE_NAME(Raw File Name)=DS1-095-017-.mzXML; Pellet size=10 Million; Analysis=Metabolomics; Polarity=Negative SUBJECT_SAMPLE_FACTORS Donor D DS1-095-018- Sample source:Red blood cells | Assay:01CF19 RAW_FILE_NAME(Raw File Name)=DS1-095-018-.mzXML; Pellet size=10 Million; Analysis=Metabolomics; Polarity=Negative SUBJECT_SAMPLE_FACTORS Donor 1 DS1-095-001L+ Sample source:cultured reticulocytes | Assay:04CF29 RAW_FILE_NAME(Raw File Name)=DS1-095-001L+.RAW; Pellet size=10 Million; Analysis=Lipidomics; Polarity=Positive SUBJECT_SAMPLE_FACTORS Donor 1 DS1-095-002L+ Sample source:cultured reticulocytes | Assay:04CF29 RAW_FILE_NAME(Raw File Name)=DS1-095-002L+.RAW; Pellet size=10 Million; Analysis=Lipidomics; Polarity=Positive SUBJECT_SAMPLE_FACTORS Donor 4 DS1-095-003L+ Sample source:cultured reticulocytes | Assay:04CF29 RAW_FILE_NAME(Raw File Name)=DS1-095-003L+.RAW; Pellet size=10 Million; Analysis=Lipidomics; Polarity=Positive SUBJECT_SAMPLE_FACTORS Donor 4 DS1-095-004L+ Sample source:cultured reticulocytes | Assay:04CF29 RAW_FILE_NAME(Raw File Name)=DS1-095-004L+.RAW; Pellet size=10 Million; Analysis=Lipidomics; Polarity=Positive SUBJECT_SAMPLE_FACTORS Donor 5 DS1-095-005L+ Sample source:cultured reticulocytes | Assay:04CF29 RAW_FILE_NAME(Raw File Name)=DS1-095-005L+.RAW; Pellet size=10 Million; Analysis=Lipidomics; Polarity=Positive SUBJECT_SAMPLE_FACTORS Donor 5 DS1-095-006L+ Sample source:cultured reticulocytes | Assay:04CF29 RAW_FILE_NAME(Raw File Name)=DS1-095-006L+.RAW; Pellet size=10 Million; Analysis=Lipidomics; Polarity=Positive SUBJECT_SAMPLE_FACTORS Donor A DS1-095-007L+ Sample source:cultured reticulocytes | Assay:01CF19 RAW_FILE_NAME(Raw File Name)=DS1-095-007L+.RAW; Pellet size=10 Million; Analysis=Lipidomics; Polarity=Positive SUBJECT_SAMPLE_FACTORS Donor C DS1-095-008L+ Sample source:cultured reticulocytes | Assay:01CF19 RAW_FILE_NAME(Raw File Name)=DS1-095-008L+.RAW; Pellet size=10 Million; Analysis=Lipidomics; Polarity=Positive SUBJECT_SAMPLE_FACTORS Donor D DS1-095-009L+ Sample source:cultured reticulocytes | Assay:01CF19 RAW_FILE_NAME(Raw File Name)=DS1-095-009L+.RAW; Pellet size=10 Million; Analysis=Lipidomics; Polarity=Positive SUBJECT_SAMPLE_FACTORS Donor A DS1-095-010L+ Sample source:cultured reticulocytes | Assay:01CF19 RAW_FILE_NAME(Raw File Name)=DS1-095-010L+.RAW; Pellet size=10 Million; Analysis=Lipidomics; Polarity=Positive SUBJECT_SAMPLE_FACTORS Donor C DS1-095-011L+ Sample source:cultured reticulocytes | Assay:01CF19 RAW_FILE_NAME(Raw File Name)=DS1-095-011L+.RAW; Pellet size=10 Million; Analysis=Lipidomics; Polarity=Positive SUBJECT_SAMPLE_FACTORS Donor D DS1-095-012L+ Sample source:cultured reticulocytes | Assay:01CF19 RAW_FILE_NAME(Raw File Name)=DS1-095-012L+.RAW; Pellet size=10 Million; Analysis=Lipidomics; Polarity=Positive SUBJECT_SAMPLE_FACTORS Donor A DS1-095-013L+ Sample source:cultured reticulocytes | Assay:01CF19 RAW_FILE_NAME(Raw File Name)=DS1-095-013L+.RAW; Pellet size=10 Million; Analysis=Lipidomics; Polarity=Positive SUBJECT_SAMPLE_FACTORS Donor C DS1-095-014L+ Sample source:cultured reticulocytes | Assay:01CF19 RAW_FILE_NAME(Raw File Name)=DS1-095-014L+.RAW; Pellet size=10 Million; Analysis=Lipidomics; Polarity=Positive SUBJECT_SAMPLE_FACTORS Donor D DS1-095-015L+ Sample source:cultured reticulocytes | Assay:01CF19 RAW_FILE_NAME(Raw File Name)=DS1-095-015L+.RAW; Pellet size=10 Million; Analysis=Lipidomics; Polarity=Positive SUBJECT_SAMPLE_FACTORS Donor A DS1-095-016L+ Sample source:Red blood cells | Assay:01CF19 RAW_FILE_NAME(Raw File Name)=DS1-095-016L+.RAW; Pellet size=10 Million; Analysis=Lipidomics; Polarity=Positive SUBJECT_SAMPLE_FACTORS Donor C DS1-095-017L+ Sample source:Red blood cells | Assay:01CF19 RAW_FILE_NAME(Raw File Name)=DS1-095-017L+.RAW; Pellet size=10 Million; Analysis=Lipidomics; Polarity=Positive SUBJECT_SAMPLE_FACTORS Donor D DS1-095-018L+ Sample source:Red blood cells | Assay:01CF19 RAW_FILE_NAME(Raw File Name)=DS1-095-018L+.RAW; Pellet size=10 Million; Analysis=Lipidomics; Polarity=Positive SUBJECT_SAMPLE_FACTORS Donor 1 DS1-095-001L- Sample source:cultured reticulocytes | Assay:04CF29 RAW_FILE_NAME(Raw File Name)=DS1-095-001L-.RAW; Pellet size=10 Million; Analysis=Lipidomics; Polarity=Negative SUBJECT_SAMPLE_FACTORS Donor 1 DS1-095-002L- Sample source:cultured reticulocytes | Assay:04CF29 RAW_FILE_NAME(Raw File Name)=DS1-095-002L-.RAW; Pellet size=10 Million; Analysis=Lipidomics; Polarity=Negative SUBJECT_SAMPLE_FACTORS Donor 4 DS1-095-003L- Sample source:cultured reticulocytes | Assay:04CF29 RAW_FILE_NAME(Raw File Name)=DS1-095-003L-.RAW; Pellet size=10 Million; Analysis=Lipidomics; Polarity=Negative SUBJECT_SAMPLE_FACTORS Donor 4 DS1-095-004L- Sample source:cultured reticulocytes | Assay:04CF29 RAW_FILE_NAME(Raw File Name)=DS1-095-004L-.RAW; Pellet size=10 Million; Analysis=Lipidomics; Polarity=Negative SUBJECT_SAMPLE_FACTORS Donor 5 DS1-095-005L- Sample source:cultured reticulocytes | Assay:04CF29 RAW_FILE_NAME(Raw File Name)=DS1-095-005L-.RAW; Pellet size=10 Million; Analysis=Lipidomics; Polarity=Negative SUBJECT_SAMPLE_FACTORS Donor 5 DS1-095-006L- Sample source:cultured reticulocytes | Assay:04CF29 RAW_FILE_NAME(Raw File Name)=DS1-095-006L-.RAW; Pellet size=10 Million; Analysis=Lipidomics; Polarity=Negative SUBJECT_SAMPLE_FACTORS Donor A DS1-095-007L- Sample source:cultured reticulocytes | Assay:01CF19 RAW_FILE_NAME(Raw File Name)=DS1-095-007L-.RAW; Pellet size=10 Million; Analysis=Lipidomics; Polarity=Negative SUBJECT_SAMPLE_FACTORS Donor C DS1-095-008L- Sample source:cultured reticulocytes | Assay:01CF19 RAW_FILE_NAME(Raw File Name)=DS1-095-008L-.RAW; Pellet size=10 Million; Analysis=Lipidomics; Polarity=Negative SUBJECT_SAMPLE_FACTORS Donor D DS1-095-009L- Sample source:cultured reticulocytes | Assay:01CF19 RAW_FILE_NAME(Raw File Name)=DS1-095-009L-.RAW; Pellet size=10 Million; Analysis=Lipidomics; Polarity=Negative SUBJECT_SAMPLE_FACTORS Donor A DS1-095-010L- Sample source:cultured reticulocytes | Assay:01CF19 RAW_FILE_NAME(Raw File Name)=DS1-095-010L-.RAW; Pellet size=10 Million; Analysis=Lipidomics; Polarity=Negative SUBJECT_SAMPLE_FACTORS Donor C DS1-095-011L- Sample source:cultured reticulocytes | Assay:01CF19 RAW_FILE_NAME(Raw File Name)=DS1-095-011L-.RAW; Pellet size=10 Million; Analysis=Lipidomics; Polarity=Negative SUBJECT_SAMPLE_FACTORS Donor D DS1-095-012L- Sample source:cultured reticulocytes | Assay:01CF19 RAW_FILE_NAME(Raw File Name)=DS1-095-012L-.RAW; Pellet size=10 Million; Analysis=Lipidomics; Polarity=Negative SUBJECT_SAMPLE_FACTORS Donor A DS1-095-013L- Sample source:cultured reticulocytes | Assay:01CF19 RAW_FILE_NAME(Raw File Name)=DS1-095-013L-.RAW; Pellet size=10 Million; Analysis=Lipidomics; Polarity=Negative SUBJECT_SAMPLE_FACTORS Donor C DS1-095-014L- Sample source:cultured reticulocytes | Assay:01CF19 RAW_FILE_NAME(Raw File Name)=DS1-095-014L-.RAW; Pellet size=10 Million; Analysis=Lipidomics; Polarity=Negative SUBJECT_SAMPLE_FACTORS Donor D DS1-095-015L- Sample source:cultured reticulocytes | Assay:01CF19 RAW_FILE_NAME(Raw File Name)=DS1-095-015L-.RAW; Pellet size=10 Million; Analysis=Lipidomics; Polarity=Negative SUBJECT_SAMPLE_FACTORS Donor A DS1-095-016L- Sample source:Red blood cells | Assay:01CF19 RAW_FILE_NAME(Raw File Name)=DS1-095-016L-.RAW; Pellet size=10 Million; Analysis=Lipidomics; Polarity=Negative SUBJECT_SAMPLE_FACTORS Donor C DS1-095-017L- Sample source:Red blood cells | Assay:01CF19 RAW_FILE_NAME(Raw File Name)=DS1-095-017L-.RAW; Pellet size=10 Million; Analysis=Lipidomics; Polarity=Negative SUBJECT_SAMPLE_FACTORS Donor D DS1-095-018L- Sample source:Red blood cells | Assay:01CF19 RAW_FILE_NAME(Raw File Name)=DS1-095-018L-.RAW; Pellet size=10 Million; Analysis=Lipidomics; Polarity=Negative #COLLECTION CO:COLLECTION_SUMMARY For flow cytometry, cells were fixed (1% paraformaldehyde, 0.0075% CO:COLLECTION_SUMMARY glutaraldehyde) to prevent antibody agglutination, except for assays using CO:COLLECTION_SUMMARY GLUT1.RBD. Samples were labelled with primary antibodies in PBSAG (PBS with 1% CO:COLLECTION_SUMMARY (w/v) Glucose and 0.5%(w/v) |Bovine Serum Albumin (BSA)) supplemented with extra CO:COLLECTION_SUMMARY 1%(w/v) BSA for 30min, in the dark. METAFORA’s GLUT1.RBD was incubated at CO:COLLECTION_SUMMARY 37°C and all remaining antibodies incubated at 4°C. Cells were washed 2x PBSAG CO:COLLECTION_SUMMARY and, if required, incubated with appropriate secondary antibody under the same CO:COLLECTION_SUMMARY conditions as described for primary. Cells were washed 2x with PBSAG and CO:COLLECTION_SUMMARY analysed on a Miltenyi MACSQuant 10 flow cytometer. Data was analysed using CO:COLLECTION_SUMMARY FlowJo v10.7 (FlowJo). Reticulocytes were identified by gating on the CO:COLLECTION_SUMMARY Hoechst-negative population. 4 Cells were sorted using a BD Influx Cell Sorter CO:COLLECTION_SUMMARY (BD Biosciences). BEL-A CRISPR edited populations were single cell sorted based CO:COLLECTION_SUMMARY on viability (DRAQ7 negativity). Primary GLUT1 KO cultures were sorted on days 6 CO:COLLECTION_SUMMARY or 7 of differentiation using DRAQ7 and the eGFP-fused GLUT1.RBD to purify the CO:COLLECTION_SUMMARY negative population with a gate based on non-targeting guide control cells. CO:SAMPLE_TYPE Cultured cells #TREATMENT TR:TREATMENT_SUMMARY Filtered reticulocytes (1-2 x105/well) were incubated in decreasing NaCl TR:TREATMENT_SUMMARY concentrations (0.9–0%) for 10min at 37°C. Lysis was stopped by adding 4x TR:TREATMENT_SUMMARY volume of PBSAG. Live cells, considered as having a normal FSC/SSC profile as TR:TREATMENT_SUMMARY defined by the 0.9% NaCl control, were counted by flow cytometry using the TR:TREATMENT_SUMMARY MACSQuant10 #SAMPLEPREP SP:SAMPLEPREP_SUMMARY Extraction of metabolites and lipids from red cell pellets was as follows: 36 SP:SAMPLEPREP_SUMMARY µL of cold MeOH:MeCN:H2O (5:3:2, v:v:v) was added to all pellets (pellet size SP:SAMPLEPREP_SUMMARY pre-determined to be 10 million prior to extraction). Samples were vortexed at SP:SAMPLEPREP_SUMMARY 4°C for 30 minutes. Insoluble material was pelleted by centrifugation (18000g, SP:SAMPLEPREP_SUMMARY 10 min) and supernatants were isolated for analysis by UHPLC-MS for metabolomics SP:SAMPLEPREP_SUMMARY and lipidomics. #CHROMATOGRAPHY CH:CHROMATOGRAPHY_SUMMARY Lipidomics Positive CH:CHROMATOGRAPHY_TYPE Reversed phase CH:INSTRUMENT_NAME Thermo Vanquish CH:COLUMN_NAME Phenomenex Kinetex C18 (30 x 2.1mm, 1.7um) CH:SOLVENT_A 75:25 H2O:ACN 5mM NH4OAc CH:SOLVENT_B 90:10 iPrOH:ACN 5mM NH4OAc CH:FLOW_GRADIENT 0 min - 0.3ml/min - 30%B, 3 min - 0.3ml/min - 100%B, 4.2min - 0.3ml/min - 100%B, CH:FLOW_GRADIENT 4.3min - 0.4ml/min - 30%B, 4.9min - 0/4ml/min - 30%B, 5 min - 0.3ml/min 30%B CH:FLOW_RATE 0.3-0.4ml/min CH:COLUMN_TEMPERATURE 50 #ANALYSIS AN:ANALYSIS_TYPE MS #MS MS:INSTRUMENT_NAME Thermo Q Exactive Orbitrap MS:INSTRUMENT_TYPE Orbitrap MS:MS_TYPE ESI MS:ION_MODE POSITIVE MS:MS_COMMENTS Lipidsearch used for data analysis #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS area MS_METABOLITE_DATA_START Samples DS1-095-001L+ DS1-095-002L+ DS1-095-003L+ DS1-095-004L+ DS1-095-005L+ DS1-095-006L+ DS1-095-007L+ DS1-095-008L+ DS1-095-009L+ DS1-095-010L+ DS1-095-011L+ DS1-095-012L+ DS1-095-013L+ DS1-095-014L+ DS1-095-015L+ DS1-095-016L+ DS1-095-017L+ DS1-095-018L+ Factors Sample source:cultured reticulocytes | Assay:04CF29 Sample source:cultured reticulocytes | Assay:04CF29 Sample source:cultured reticulocytes | Assay:04CF29 Sample source:cultured reticulocytes | Assay:04CF29 Sample source:cultured reticulocytes | Assay:04CF29 Sample source:cultured reticulocytes | Assay:04CF29 Sample source:cultured reticulocytes | Assay:01CF19 Sample source:cultured reticulocytes | Assay:01CF19 Sample source:cultured reticulocytes | Assay:01CF19 Sample source:cultured reticulocytes | Assay:01CF19 Sample source:cultured reticulocytes | Assay:01CF19 Sample source:cultured reticulocytes | Assay:01CF19 Sample source:cultured reticulocytes | Assay:01CF19 Sample source:cultured reticulocytes | Assay:01CF19 Sample source:cultured reticulocytes | Assay:01CF19 Sample source:Red blood cells | Assay:01CF19 Sample source:Red blood cells | Assay:01CF19 Sample source:Red blood cells | Assay:01CF19 AcCa(14:0) 16131 18284 7486 9442 9558 14061 7757 7793 6456 29443 36095 32847 18239 14028 20337 7153 7247 8413 AcCa(18:1) 72446 130482 17339 55533 44058 89486 75931 65759 42454 111257 149954 105512 108408 96269 125640 472431 283112 324362 AcCa(18:0) 93386 139306 22436 53488 58677 94272 85894 92182 57453 179290 242659 170351 266865 258288 282524 151447 106367 174849 AcCa(26:0) 3478 1666 2269 1000 3671 1063 1853 2095 976 3208 1286 351 2088 849 5566 2337 2936 5747 AcCa(2:0) 142898 295798 82201 188317 109990 266893 398234 579047 208741 1745788 2464155 1168607 1391416 1435432 1188965 769208 705997 871553 AcCa(16:0) 197977 212418 47743 100864 112677 147939 74801 89470 64273 268217 325035 253503 210782 178556 207253 285282 151113 208295 AcCa(17:0) 20933 22505 4001 10050 10562 15402 17890 20071 13360 33593 46575 29727 37582 36344 38989 12266 9078 18967 AcCa(20:3) 3090 6367 1113 4268 2524 5701 4035 4159 1489 9249 14526 8179 11756 16190 9610 22847 26885 24152 AcCa(20:2) 2592 4934 813 2789 1896 3758 2079 1891 851 3647 6614 4006 3415 4539 3695 13501 10819 11570 AcCa(22:5) 412 425 1 209 158 429 185 276 164 1125 1367 581 547 190 588 2563 2567 6466 AcCa(18:2) 45233 70163 11501 30452 26147 48538 51633 42849 32015 101858 111956 76050 78988 69639 66779 163096 134239 98246 AcCa(15:0) 9215 9554 2373 5094 4890 6500 8780 8901 6828 14751 20451 14158 16716 17596 17220 4488 3428 5929 AcCa(12:0) 834 1439 391 747 615 1060 1101 999 1054 6204 10886 8999 3312 2315 3753 283 607 321 AcCa(4:0) 464 3852 157 326 443 2316 6820 7084 4169 6278 24125 45218 239 8540 33318 8144 13277 12250 AcCa(17:1) 6030 7394 1166 2410 2948 4381 7410 7833 6363 17090 17679 9965 12786 10805 10804 1939 1432 2313 AcCa(16:1) 7835 11387 2600 5406 4741 6555 9980 9419 7349 25993 26688 21904 17667 15236 13690 9522 6651 8671 Cer(d18:1_16:0) 29409 9850 21983 5708 26302 6147 20321 28295 19358 35429 27753 3659 17442 7107 29302 4436 2555 13719 Cer(d18:2_24:1) 52698 19564 56440 9582 38901 7505 22885 33948 14329 53032 44400 3586 5656 2375 14015 5116 3576 37598 Cer(d18:2_16:0) 33044 22128 26131 12637 35837 16251 31445 33291 41422 74074 64566 19416 45529 27444 62840 5074 2658 7425 Cer(d18:2_18:0) 23193 8501 17782 5567 22867 5121 16798 19153 16324 39027 31148 3673 27467 15109 39784 2891 1680 8217 Cer(d18:1_22:2) 7280 2563 5850 1063 5060 951 3491 5145 2758 5858 4323 235 1337 516 3102 164 276 2199 Cer(d16:1_16:0) 6560 3770 5768 3106 5991 2564 6425 6714 9669 11274 9605 2540 5554 3000 9261 1891 149 1946 Cer(d18:2_22:0) 8992 2265 6071 1087 5072 862 3463 5505 2456 11298 10069 594 2709 850 5303 659 188 7234 Cer(d18:2_23:0) 327 1 273 96 134 1 1 108 1 400 255 137 1 1 310 121 1 1835 Cer(d18:0_24:0) 458 1 172 1 200 1 1 256 100 1 297 1 1 1 159 1 1 262 Cer(d18:1_24:1) 11015 3359 7914 2910 6077 1748 3078 5324 2624 6927 8789 4273 1720 612 2855 1219 981 13390 Cer(d18:1_18:0) 2395 632 1847 689 2212 572 1397 2186 1699 2709 1637 851 2049 1143 4246 747 876 1161 Cer(d18:1_24:0) 2848 2375 852 2061 377 1953 755 4713 824 1739 1349 2873 448 688 583 460 466 578 Ch 75270 44710 78613 27746 103569 24602 55966 63457 44675 21229 24266 9802 429069 308300 549215 13007 23067 7789 DG(16:0_18:0) 4317 4065 4009 4105 4178 4124 4524 4810 4223 3548 4155 4252 4452 4145 4268 4055 4178 4308 DG(18:1_18:2) 3212 1526 4210 1299 3345 842 2352 3615 1711 5522 5455 1041 3747 2336 7914 2772 3527 11361 DG(18:1_18:1) 1849 759 1954 584 2292 429 1274 1840 976 1750 2431 469 1384 927 2648 2049 1509 8560 DG(16:0_18:1) 2495 978 2952 477 2200 838 1489 1831 980 2337 2558 398 1183 672 2380 2160 1670 9572 DG(18:0_20:4) 6040 1504 4162 1080 4715 548 2712 3094 1162 4708 3826 247 2214 1050 3783 2055 1263 9926 DG(O-26:4) 8674 8717 6828 8056 10670 10670 11027 12259 9824 10964 10592 9800 9716 9469 9611 4526 5135 5179 DG(16:0_18:2) 3709 1661 4596 910 4147 649 2175 3251 2444 5372 5188 456 3588 1716 6292 7972 5454 23770 DG(16:0_20:4) 5975 2438 4860 1784 5620 1183 3597 4450 3226 10189 9672 1461 8496 3739 14195 4919 3980 24076 Hex1Cer(d18:1_16:0) 278 1409 1995 3117 506 2564 553 2210 2149 312 2837 3741 1908 1659 2751 731 1500 270 LPC(16:0) 1034743 997791 852188 1105310 1165565 1044123 1456435 1522962 1288473 1101174 1130496 1851952 2508155 1120865 1329693 874812 818140 967270 LPC(17:0) 159590 163646 135794 128938 189301 171087 197807 210952 61975 127199 137351 128960 223667 197635 206493 144483 172481 171767 LPC(18:0) 393797 562384 476465 756815 703088 770756 821654 655639 604475 408619 484442 597151 1859474 1609378 1055545 811847 524644 1039802 LPC(O-17:0) 7639 6732 6509 6231 8235 6981 9035 8527 7298 5448 6266 5609 14295 16717 14680 6579 5279 5943 LPC(O-17:1) 8083 8069 7986 7171 10450 9248 10321 13220 11025 6587 9956 8492 33417 35498 38989 10639 11835 11843 LPC(20:3) 17768 18997 24375 27080 22376 22630 39214 34911 35051 28551 32891 25544 42171 40139 41394 19486 27853 40318 LPC(P-18:0) 74296 69878 83848 76707 95984 71439 74024 83607 57221 50833 63664 51237 87869 84085 109147 19427 20846 25909 LPC(30:0) 5184 2311 8986 1851 1526 3921 2866 3728 1631 4464 9103 1328 8317 5424 13252 5435 1280 2627 LPC(20:0) 17364 19908 16481 16729 22769 23053 22774 28166 29679 19269 20641 15687 30689 24157 30389 16272 16971 17747 LPC(P-21:5) 58265 51819 43940 56796 56239 43577 65596 58529 52905 42837 43520 29138 65275 59091 51545 29255 40583 37061 LPC(24:1) 14469 14092 11153 12590 15335 14722 19041 18861 16312 14688 16015 13450 19012 15552 20422 5179 6910 8107 LPC(15:1) 97666 99323 65123 70391 165325 102286 82162 108563 39086 27590 21063 55278 89534 22114 79601 62807 55461 82453 LPC(P-16:0) 33318 18340 25779 30180 38963 30357 42209 48116 21161 12587 37118 25785 179757 186563 201652 9864 33160 40670 LPC(P-15:0) 8550 8137 7573 8245 9009 8200 9285 17264 9779 10353 13300 14329 51571 54089 52609 16033 16753 9252 LPC(19:0) 31730 29517 29132 28305 40133 36394 46352 47571 39335 28514 26364 22673 37071 38344 15014 37834 12272 35657 LPC(22:4) 10654 11731 10730 10214 12575 13567 13515 12563 11411 10666 11951 9973 8011 6883 7612 4811 5692 5686 LPC(23:1) 12693 10859 9032 9923 14601 12010 17102 17409 15201 12507 14742 10074 16123 13488 16554 6689 6696 8223 LPC(P-17:0) 7924 9087 7963 7171 10243 9135 10573 12872 10666 6516 9959 7471 18318 35467 20683 9513 10682 11993 LPC(P-18:1) 7585 7104 8090 9104 7391 7099 8279 8938 7398 5764 7395 6742 15566 15831 13587 3638 2802 4078 LPC(O-18:1) 19790 17008 18806 17352 21777 15327 19220 22977 16730 14159 17557 13600 41568 39893 46237 7684 9162 9533 LPC(20:2) 5926 5423 6832 6578 7338 7046 7121 9375 8747 7048 8469 6980 8920 8868 8882 8136 5571 6261 LPC(O-22:0) 5723 6805 5662 4443 9628 6295 4551 5124 3481 1992 2334 1263 2790 2370 3219 2479 1837 3532 LPC(14:0) 3575 3965 3492 3967 3876 4123 4027 3867 3460 4113 3351 2901 2554 1874 2741 1531 1581 1703 LPC(15:0) 6670 6628 3945 4659 5850 5736 8351 8799 7923 6140 8054 6534 9088 8526 10305 6671 6612 7257 LPC(O-16:0) 42098 35128 52087 42414 60515 42267 46875 51790 41064 35780 44515 37072 67682 65739 76508 17814 20332 25614 LPC(16:1) 5961 6467 6326 7171 7785 7192 10343 10237 10167 7767 8450 10046 10226 9204 11561 5705 6050 7087 LPC(O-18:0) 45455 39346 42972 33234 54819 40875 51739 57402 45516 37109 49101 38625 101175 101590 122944 26777 24138 33741 LPC(18:2) 14007 14547 20405 23073 24831 21329 26691 22817 28034 18503 18645 20443 21863 23242 22579 35601 35443 65329 LPC(20:4) 15948 18191 14172 18974 17369 20001 21949 22765 20793 22185 21054 23346 18753 17124 18708 8173 7531 11604 LPC(18:1) 551045 432998 601987 605490 537477 476566 660893 618921 630297 526506 500682 478841 583678 602043 629762 539874 676433 643790 LPE(22:6) 40581 42339 22188 51426 58389 48359 49886 44934 51086 41477 30051 20847 52985 29327 32314 56697 28306 44325 LPE(16:1) 8237 8738 8646 7580 8979 10204 15838 14102 13365 18941 12534 10104 16070 18148 13675 13159 13513 10339 LPE(18:2) 8919 9464 10409 14507 16406 12270 13170 12622 13052 10281 11379 7219 14660 12817 16804 13187 19649 17942 LPE(20:5) 2389 2422 2602 5956 3799 3633 4243 4301 4547 20646 3953 6561 11503 8937 6579 10454 6841 6332 LPG(18:2) 5242 5253 3369 4615 5672 5943 2215 4958 4900 6818 7425 4077 10335 4968 13277 414 235 548 LPS(20:4) 10347 9485 6427 7985 8924 12466 10682 8675 7274 12797 10997 8970 9570 8795 13025 7141 5608 8085 PA(O-20:2_20:4) 17808 7391 19475 6550 20398 6538 11442 14510 10541 16728 13114 2985 12932 7605 25644 7879 5815 24752 PA(O-20:2_22:4) 5320 906 2817 516 2289 0 1229 1821 757 3592 750 4 652 193 1643 114 8 2588 PA(22:5_14:0) 35644 31186 31172 28068 39668 24417 28930 26842 33203 38663 21657 13617 11415 6862 15165 10741 9766 14796 PA(22:5_16:0) 30016 16681 21775 18611 11467 14288 17573 11795 20558 14611 20122 9150 5706 7418 10779 20225 16044 31256 PA(O-18:2_20:5) 21263 17601 27319 12061 40736 16394 43329 25900 27035 3641 6447 5543 12292 4600 5977 1178 543 1767 PA(30:1_19:3) 4816 2159 3663 691 4572 308 2629 2822 1029 3817 3638 242 1804 817 5073 1 1 495 PA(O-19:2_20:4) 44848 22800 53104 15357 40182 11510 25758 36699 26105 24325 25395 5660 41587 21378 82768 20089 14829 91186 PA(22:6_18:0) 33074 42146 33763 24646 62631 35159 45417 35030 31130 27129 20546 10318 24631 18515 23196 4683 2808 8317 PA(O-20:3_18:2) 35493 14598 74992 13520 75711 21392 20824 33524 19349 74371 78444 19403 60185 64508 156645 73288 35984 117381 PA(O-20:2_22:5) 598165 318251 458287 154792 494731 102143 211520 382915 211651 286615 250912 34203 78899 40355 167686 129605 104676 410228 PA(22:6_16:0) 50057 47401 35751 34303 75404 35101 41903 39784 40485 37815 29275 17208 19871 14258 18661 5262 5293 6512 PA(O-20:3_20:5) 19087 19528 18191 16481 31197 22586 39218 28711 35763 5987 3098 2617 5154 5320 5437 1208 1211 1248 PA(O-18:2_22:5) 1146104 805477 1240757 597273 1081353 502276 734723 962605 667706 799926 795540 245698 515657 290725 874260 527062 585923 1199334 PA(21:2_4:0) 12013 15625 9296 11288 21143 14372 21003 17671 10153 14463 11194 2534 10916 6210 8670 4890 2098 5338 PA(O-20:3_16:1) 22387 10960 23585 8056 23157 6426 12089 15526 11116 28173 26780 7084 24658 17839 33372 25197 15609 68232 PC(18:1_16:0) 17181236 9616443 14418746 7161238 15607920 6070350 13644816 14701183 11363469 12912784 13193712 3396173 9461647 5275679 12702007 7685200 6681560 12439722 PC(34:1) 437342 138148 225863 133466 141340 108360 238528 353536 237934 305701 252472 60400 187586 96505 346432 166053 143405 245377 PC(34:0) 62438 1369 59959 12946 43825 6860 39914 48934 22622 130266 72263 6189 38021 10387 67130 21495 8628 125250 PC(36:4) 13618614 11187164 9090872 9107580 12279476 8329503 13303243 11882747 10065039 17931101 14553727 8617512 12210474 8246190 14947384 5239072 3797074 9360654 PC(14:1_22:3) 161187 51900 117085 57369 22440 35180 129530 66474 104500 114447 78370 31422 53916 24501 64361 29257 18145 47340 PC(36:2) 2494154 1413729 2602614 1051134 2650469 774369 2258747 2495498 2124837 2211859 1765027 380108 2209020 1164625 3419162 4719712 4475237 8339863 PC(32:0) 2109664 900468 1526354 547801 1473307 482340 1301737 1582571 807068 2436609 1533940 172291 454067 161516 709631 356463 196174 1024268 PC(38:4) 4117440 3051445 3560337 2432907 3539220 1720105 2893352 4002343 3771590 5879981 4958354 1422735 4776147 2835820 7001543 1728054 1355457 4466055 PC(36:1) 781170 307870 922771 166615 809886 109359 451481 579982 366226 1237921 708290 118127 709125 310531 1788192 620094 541808 2711942 PC(28:0) 21707 14283 9552 8464 15178 8059 10216 12938 14191 10619 8385 4545 7756 5058 9469 9658 12938 17963 PC(30:1) 35662 23012 23537 17469 28325 15294 22063 23023 26835 15699 15068 10017 13752 11327 20000 10311 13563 17818 PC(30:0) 486400 221886 290717 151885 344508 131846 232154 253493 231424 293268 168024 43485 85633 38108 136798 105712 75511 248699 PC(31:0) 94482 54840 77037 35184 75779 30142 78746 81241 54013 76363 73436 20978 73808 34112 118057 8155 16126 59556 PC(O-32:0) 20729 5189 23312 3805 24444 3184 14126 20788 8574 34058 22825 1517 15623 5641 26634 10649 4803 58806 PC(32:2) 217520 125751 154014 182813 189947 87459 126440 128906 126146 116824 98318 43585 109568 69831 223530 108723 114491 130452 PC(O-15:1_18:1) 13015 2964 12279 2160 7224 1784 3106 7271 8215 15540 5047 4837 63457 43326 96777 4411 6853 42019 PC(33:3) 54207 24388 46024 37441 77327 27449 101487 109243 53952 73618 74847 29332 87780 70249 81580 9782 10989 8706 PC(17:3_16:0) 163294 71859 363930 114927 188139 57760 140852 192478 93795 272098 286335 86859 283949 208989 449626 521710 802126 685133 PC(O-34:2) 201299 144853 258348 125947 315165 148469 215193 263904 200790 161335 256458 87017 438384 322116 489968 287738 115385 230030 PC(O-34:1) 1539814 690809 1705734 444028 1889669 356717 1024996 1472240 851196 1011518 1141989 138887 1067458 558776 3952329 154007 125966 581803 PC(P-15:2_20:4) 122966 97629 144327 30328 131294 77104 42599 41638 53837 115640 116813 42920 82047 50019 114383 244297 27142 462074 PC(O-34:0) 7020 1353 5569 691 2462 1 1034 2541 956 7743 7063 424 3853 1725 9050 1329 1 5880 PC(O-35:6) 13350 6053 17831 6466 19466 5264 5697 9448 5025 1797 3146 1571 4782 8098 11938 81 697 46 PC(O-13:1_22:5) 1146860 806435 1233412 645280 1081244 501732 737428 963177 667451 853995 795346 265761 515577 291429 874739 526678 539742 1199996 PC(34:4) 243529 181314 175257 148814 212604 130927 206279 208555 184653 287317 188428 96498 159176 95384 206669 46185 34012 85838 PC(34:2) 11002354 7650771 10085742 6404212 12024952 6468831 10154612 11030035 10116300 6731250 6499806 2915266 7954849 5856469 10018662 15289629 14527506 15787535 PC(35:6) 400025 316912 337735 273792 492521 261636 504376 499158 435362 475665 327514 141236 279315 150634 392521 520787 422227 741383 PC(O-36:6) 14072 9071 9 4 15605 7709 12082 6 11447 13753 3 5346 5 1 11 11919 32 18 PC(O-14:1_22:5) 20030 10019 13865 8332 18412 4604 12207 21666 9875 13620 13476 2262 23923 20395 60306 11901 9857 39062 PC(35:5) 1466875 1013825 1341420 694934 1488271 653458 1255412 1288947 1228350 1199416 1034912 358075 850091 415793 1102352 771818 884085 1410301 PC(35:4) 135392 93403 102003 76660 103779 62319 109707 124240 91939 158755 118430 62551 159897 97177 221529 33354 248013 100781 PC(19:4_16:0) 1831896 935740 2008985 721299 1508793 494002 1368637 1388885 1028585 1837130 1317519 274346 1026658 471728 1673700 412862 360013 1018693 PC(O-36:4) 1042643 920360 1223253 1081080 1614356 759476 1114254 1868277 1097488 1193028 977388 470731 1683244 1011366 1905630 394023 319065 1021282 PC(O-14:1_22:3) 27459 24869 28238 12452 61725 29182 41661 50055 34951 18376 38924 14230 64959 31302 83785 9883 8641 21256 PC(35:2) 228987 157558 213951 99797 234654 101473 207310 251904 211769 186287 178200 48787 324212 187366 373277 294946 255452 496556 PC(O-15:2_22:6) 171509 134546 161793 126637 155023 114607 141612 176293 145940 110013 94004 41100 86908 110516 145536 185397 155191 244332 PC(O-37:8) 68261 44672 64323 41468 54913 27478 47064 58884 46956 48644 55777 15972 1597 879 956 46879 39125 94850 PC(36:6) 34485 28540 23049 33503 54074 21111 75145 70608 34622 71026 52947 64706 44391 29383 91438 18247 11426 22438 PC(O-35:2) 3225 897 2220 518 2037 0 1007 1416 33265 1855 1194 242 870 30302 1400 117 9 2249 PC(O-37:4) 75537 42061 90084 38613 101187 28015 68365 88263 61221 52712 105980 18821 236804 124507 326670 11600 5061 48083 PC(O-15:1_22:3) 48647 16958 33303 5686 30602 6532 17272 25567 9438 52692 5 5042 47885 16263 50948 6774 2620 77 PC(36:3) 3944007 2756561 3752712 2621995 4537974 1952841 4151566 4515103 3291326 4254619 4778273 1174895 4735319 3617156 5906894 4299558 4069921 5275375 PC(O-38:6) 604718 668676 856774 682016 916095 534281 623062 784003 658126 603900 686562 276182 506102 442375 724212 120373 74140 358359 PC(O-38:5) 4102013 3642395 3457171 2875650 5087469 2483199 3714525 4596457 3332526 2447639 3200232 1093511 3114163 1585323 4136672 357399 307431 786689 PC(37:2) 8631 1595 14558 850 8551 3059 9786 10627 8990 13355 7960 2224 9598 8467 33073 12153 9727 28884 PC(22:6_16:1) 28266 26151 20062 20335 25632 23388 34114 31752 29817 43468 34008 20573 32954 25117 33248 38567 18836 52247 PC(O-39:7) 26623 10958 18968 9415 15947 4467 11349 18591 6002 17087 12826 1834 5023 4898 8875 4530 3002 14145 PC(22:6_16:0) 1455672 939189 893374 860193 1452085 860009 1510884 1742000 1365411 1741604 1455913 563946 1357630 904443 1675813 1428464 1031237 2942101 PC(38:6) 1431947 953543 911256 502669 1425705 764125 348209 1711869 1308716 829750 1416350 563160 796878 763269 1075272 944651 1010825 2911705 PC(O-17:1_22:4) 30897 16114 24038 10554 33707 7858 20208 29667 19571 11683 17569 3639 26725 11801 46740 266 204 2531 PC(38:2) 42543 15294 49733 9633 35734 5793 29540 25828 12522 56024 30404 4247 30325 13785 57728 22057 17087 77655 PC(O-40:4) 159975 61717 142139 29804 129697 18290 60713 109097 66347 85722 88086 12198 78990 30625 189418 5799 5341 47118 PC(17:3_23:5) 43611 49232 36236 47844 45942 42169 73279 57104 51605 168271 140432 76591 120875 67936 133291 49540 33172 97068 PC(40:8) 25637 38795 32317 15902 28879 37838 45263 23183 52028 10826 46842 15916 11720 36795 39038 27432 16037 30810 PC(40:4) 466218 208106 393237 141464 305489 66625 321834 361373 334940 537582 376853 33575 129884 96655 434873 51919 32526 232817 PC(O-42:2) 1 1 108 1 141 1 1 1 1 1 1 1 1 1 139 1 1 1935 PC(42:2) 203 1 166 1 1 1 1 1 1 195 150 1 1 1 180 124 1 1348 PC(44:2) 1 1 1 1 1 1 1 115 1 1 1 1 114 1 180 1 1 300 PC(O-32:1) 79084 59919 73891 32250 102271 37650 54230 77299 59263 60453 86333 28015 135298 95555 158570 20141 28095 55946 PC(O-13:1_20:5) 21687 16930 25110 14189 22157 11447 15014 21456 18418 23982 20189 10696 28254 22710 45399 64014 25818 144933 PC(32:1) 1803584 978400 1598515 721216 1713945 556814 1346884 1508563 1286801 948945 998280 371722 909277 571386 1445190 448602 526029 916817 PC(33:2) 180449 129709 305433 104202 337448 103513 169824 206397 162375 237027 218151 75116 197537 158405 473218 405546 425715 764740 PC(O-35:7) 28156 19488 31168 29866 37713 20188 23186 28184 35767 5983 3030 2620 5149 2963 5433 1209 1210 1247 PC(O-15:2_20:5) 133930 102101 137973 95451 131288 77172 109796 144389 112950 130647 116833 42920 82065 50023 114525 244289 116030 462080 PC(35:1) 337703 151337 285251 97728 263711 51982 223572 258545 195621 332489 249272 39116 304533 164641 516091 123856 89322 356668 PC(O-15:2_22:3) 550212 172445 393992 105748 356347 60081 187801 106012 88242 345385 251730 27090 113243 43172 215328 34448 24252 230458 PC(37:8) 70904 80660 59997 79670 92707 83485 130756 107130 77584 186861 117027 57252 130890 56318 124610 58400 48478 75841 PC(37:6) 265158 158204 266621 137116 275641 110938 256334 276380 231363 288298 278081 60794 127921 96048 187326 117833 103952 182944 PC(37:5) 48137 36814 33442 21705 40522 22098 43009 46273 32381 46877 52417 18686 1522 285 128561 14553 5 42383 PC(19:4_18:1) 280490 133433 275907 90084 177138 71640 152025 173829 186320 217238 152408 38252 80718 44192 151952 64095 74630 232113 PC(37:4) 429900 313843 326730 236856 293245 158841 346128 363437 276735 598779 475560 156856 670599 334787 862612 99660 69803 305672 PC(O-38:4) 1618751 874657 1793551 507837 2209323 362684 1145323 1531020 954263 1258965 1778614 221959 2518508 1405804 4565086 125195 105344 464142 PC(O-15:1_22:6) 218573 273327 393967 208953 392139 134521 280506 371076 237400 344945 250473 74595 172571 65244 214075 106977 120631 164148 PC(37:3) 7378 1621 8359 1436 64579 986 2110 3674 2439 38768 12468 1865 183507 111189 168391 46644 29820 34313 PC(38:5) 2170720 1944588 1692543 1667758 1908798 1324617 2212307 1885893 2018833 2395380 1992881 1010639 1939747 1253202 1973637 890971 861572 2291480 PC(39:6) 27365 12208 27855 9389 30592 6876 25191 30687 13733 37416 31663 4177 18416 5435 22516 12876 12717 42325 PC(40:7) 149336 127880 145999 137239 165205 111330 232947 240443 169816 363251 317155 145479 356222 264124 421691 91654 99495 201262 PC(22:7_18:0) 50845 65247 52542 59691 47928 48617 113173 103841 99881 97546 82389 66060 30774 124214 115353 50006 29663 19061 PC(40:5) 353229 246519 283666 200512 270350 112056 392707 361128 405957 468751 310188 91980 190176 104467 311875 112263 87739 261505 PC(40:1) 133 1 112 1 1 1 1 1 1 219 1 1 1 1 1 1 1 377 PC(O-42:4) 7778 2804 7872 979 7412 945 2214 5438 2315 4341 4191 326 2136 654 5354 610 474 5777 PC(O-44:6) 29483 12326 31321 8157 34970 4349 17856 27475 11889 11397 10814 1543 3618 3570 7005 5760 7626 12052 PC(O-44:4) 1013 1 1086 1 471 1 1 260 1 404 791 1 1 1 295 1 1 920 PC(33:1) 330627 209052 264712 153172 277780 114382 245561 287576 245230 254370 262346 76296 390333 221658 548079 140766 108287 343167 PC(O-36:1) 1722349 796859 1793251 467072 2161271 340399 1017064 1584027 953704 1306527 1615996 258873 2536103 1314453 4371508 142918 109195 593094 PC(O-39:8) 62570 31119 37388 26999 49785 28852 41790 49545 33923 42902 35018 4770 25945 12038 29554 42742 42464 78978 PC(O-36:2) 1537534 756117 1300008 432317 1915998 362416 863278 1376570 750123 718351 883580 168944 1161389 659859 2280381 112286 115598 393665 PC(O-36:3) 795879 467243 817419 316854 424535 368848 573909 899957 550471 375830 811532 137914 1521355 1240253 2340654 200312 219798 649040 PC(37:1) 387 586 1482 204 509 1 746 1005 960 3023 476 273 1080 972 1735 2749 1609 9952 PC(O-40:6) 1039623 539161 706439 476090 952772 381268 572927 1197677 627267 259887 487263 117119 356356 112812 641158 37958 35810 172959 PC(O-42:1) 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 PC(20:1) 13141 15686 10144 9008 23946 12855 17170 17156 11455 14921 15716 4935 31056 20639 26507 4091 3624 6109 PC(21:4) 5228 3642 5143 1700 2109 3021 3388 3458 2908 4834 3580 2917 1400 3821 1461 968 1655 1812 PC(24:0) 61207 56111 53959 57298 69273 59340 83613 87874 74735 66181 71668 59102 85312 81273 73256 32569 37316 37678 PC(25:0) 46955 45015 37680 38369 49582 41010 64692 70149 60118 46390 48949 38718 72734 57361 65887 21382 16521 20096 PC(26:0) 146693 113109 106077 93770 150514 105168 196188 209960 188302 124834 135852 100911 206631 173602 214086 27983 28873 29943 PC(O-13:1_20:4) 442586 285800 577894 289160 480521 188256 381960 496625 404086 392009 293865 116209 255401 140290 394462 21888 254351 1131808 PC(32:3) 21820 14037 15756 12476 19372 8803 18094 50237 27852 14368 12639 7238 15873 8700 19880 4503 7237 5465 PC(34:5) 18834 18013 16373 13931 18131 12419 22857 26322 25596 19303 14135 11322 26376 17121 34684 6759 3818 11636 PC(18:3_16:0) 78455 59084 54202 51917 85334 57529 136537 140862 118669 87511 87742 74235 147974 111788 138417 16838 15906 16127 PC(34:3) 721134 441148 648810 395672 790744 325520 656288 766915 758752 534067 472820 196640 560372 404742 716335 461219 418979 702658 PC(O-36:5) 25168 22600 36030 19336 30058 27006 26903 42504 31139 33992 54546 28841 77924 70348 103953 25466 6899 34554 PC(37:7) 129096 133358 140268 89208 191457 126531 230060 173006 202202 247922 304259 115404 214338 186843 314321 148398 140597 183533 PC(O-40:7) 279601 295099 237608 93798 326785 193403 197404 363467 256738 145350 223832 91987 84115 105230 157747 6184 8865 23204 PC(40:6) 270053 250447 195648 245778 151762 154780 355625 227372 224929 419025 333046 122674 259120 210709 393850 303080 198649 640644 PC(40:3) 20239 6429 23578 6392 16404 2871 10285 14209 9190 37364 26645 2944 15756 5612 45100 1374 1772 9983 PC(O-42:5) 57721 23089 59263 14637 81744 11117 36709 53626 29941 22782 14671 2491 5038 3748 13738 3172 3001 19441 PC(42:8) 37391 47561 25395 40775 30975 36213 75984 61229 46759 164079 130910 91547 51784 32407 72098 332 1 9028 PC(42:6) 33078 23668 35489 25300 33613 12906 41035 45293 34057 46604 41993 9545 23117 14150 36757 3848 3058 11215 PC(O-44:5) 16737 5620 19343 3732 21602 2507 9899 12018 5650 8068 7051 805 1666 748 5201 2794 981 11551 PC(20:0) 14207 17671 11141 10937 23861 17482 22267 13825 12658 13604 14482 10421 16243 8301 11948 6685 6520 7552 PC(O-37:6) 52815 31846 82992 39027 63681 34792 61834 52233 44472 88676 56838 14564 29355 14486 48386 42 3 1573 PC(O-39:10) 81220 80712 50912 70071 68914 63344 84280 79754 64659 93438 84292 61244 93965 72145 78363 13495 18376 37482 PC(O-40:3) 6374 3358 11549 616 5582 1073 1800 6713 1685 9876 9761 1012 11058 5061 27003 605 700 2928 PC(O-42:6) 64084 49437 98268 30274 113146 20253 62656 67185 51037 23481 34124 4438 4756 4841 17717 2784 1637 6161 PC(O-43:12) 2624 11548 5346 6907 9459 9279 3312 5411 320 12595 18225 24729 21733 3914 1763 294 2525 2261 PC(42:10) 5849 7036 3763 5343 5166 4650 9802 9149 6036 33067 25733 12772 12024 6537 13571 5922 4550 16707 PC(42:4) 6711 2891 8002 2730 4529 1452 5028 4287 3302 13964 7091 726 2830 562 8297 923 484 7815 PC(44:8) 6664 1920 5658 7331 3416 4755 12400 3315 17306 31343 22621 10856 2266 619 7883 189 1 1 PC(O-49:12) 4416 707 4144 3094 8221 1750 13803 4530 10255 8990 253 2102 13752 10036 13291 183 1 992 PC(22:1) 33135 28644 20220 21093 34616 24178 41020 40887 34792 28186 26466 17386 43957 33499 40777 4142 4411 6581 PC(23:0) 13195 11992 14344 11979 14501 12472 18414 17721 18494 18022 18152 15534 18929 17689 19709 6770 8292 8036 PC(O-16:2_18:2) 14027 12073 16476 8889 22664 7768 29111 30449 26993 18326 23254 8648 59277 37060 72319 10334 1427 16126 PC(O-35:4) 7954 5665 9308 97 10776 75 10827 14559 10391 7945 11488 7502 78971 67490 100197 6285 2982 13696 PC(35:7) 15653 21254 14770 13879 49531 19272 8237 66446 60641 20636 26255 38647 30678 27875 48782 46591 55212 15526 PC(38:3) 494107 188226 763580 207794 482950 84439 376348 558322 292022 828942 825194 108795 994344 533860 1628298 270046 234355 1107666 PC(O-40:5) 904003 458838 636982 257370 814441 155138 418378 627035 407414 292655 292707 52244 184564 84880 352148 28162 25725 119830 PC(22:0) 37493 44003 35938 35500 47756 43765 50764 57148 49855 46808 57752 38128 52765 44486 45382 19644 20753 20959 PC(O-45:13) 18505 12773 7198 13754 24278 16322 19874 13399 7285 13304 8099 11445 31331 12011 12316 4508 3300 6219 PC(26:1) 30623 25059 22212 13792 30865 23676 37422 42487 35725 29813 34972 27350 36448 36020 38870 12942 13115 13046 PC(31:1) 18151 11745 16346 8465 16852 5633 14621 17808 17287 11505 14611 4508 13158 15606 34936 6397 6512 16821 PC(O-33:1) 11971 6419 18740 6038 19850 3692 9462 11780 10910 6283 16273 4431 88714 54636 107421 2467 2541 4390 PC(33:4) 2424625 1878122 2185901 1366289 2015248 1080274 1906468 2074958 1553169 2474029 2008978 667560 1057069 526512 1605736 929742 942258 1556269 PC(O-18:4_17:1) 974 1492 5071 1196 3489 2799 3612 9167 771 5072 3001 1197 33710 24296 61865 950 872 11641 PC(35:3) 90294 44430 64140 37477 78375 38601 69182 81554 71036 82644 83197 22695 122192 102110 130067 417 88 751 PC(36:5) 10172 12194 8371 16870 7206 10109 12979 11102 14019 16091 14682 15407 13831 18273 13277 44364 49000 35878 PC(38:1) 6549 3508 8767 264 6720 1490 4564 7113 4540 3478 3241 676 80691 37816 147455 1661 908 12005 PC(39:5) 17388 14849 10145 17572 12437 8041 18397 13870 21401 21736 20664 7547 4060 1209 29876 1058 5092 24042 PC(38:8) 3658 11702 1724 12567 1905 8614 11214 10321 12050 8059 13791 15408 3215 15863 3287 13448 7710 10905 PC(42:12) 10958 8658 10982 6948 14816 7125 3546 293 587 2256 5986 157 25855 9716 21452 305 772 1045 PC(P-33:5) 21549 17177 24952 14187 24360 10816 14985 22363 19662 27212 20325 10712 28255 22715 41147 69134 25852 144897 PC(O-35:1) 16406 6197 17283 3185 23182 2306 9160 13372 6548 11289 15129 2361 37315 17615 80920 497 674 6401 PC(42:9) 12788 21682 9281 19093 17260 15060 21121 26185 20886 83499 66006 33733 23254 12510 34134 7153 5586 13671 PC(O-45:12) 336 23132 3681 28592 26325 36 37204 1085 2995 46 3466 550 17377 1539 53980 1020 103 7203 PC(O-38:3) 638 31276 114398 13313 53866 11858 19398 48211 18221 72659 191419 16889 280123 243389 634653 8744 9047 25878 PC(O-39:9) 38103 15698 14025 21358 10661 13992 22791 14141 7353 10063 21262 5336 6605 3968 5989 2870 15544 12444 PC(29:1) 59825 36113 71426 25205 59989 21329 37642 43253 46541 59996 56036 20179 31621 20246 52895 14160 14783 20354 PC(P-9:0_20:1) 2984 2061 2903 2235 2665 1220 1982 3138 2625 2228 962 311 3129 1853 6053 7962 2038 18646 PC(31:2) 191600 106405 229217 83421 216830 73341 131175 139505 148223 181537 148506 48355 163295 109608 288947 275323 276705 358359 PC(39:4) 29255 16597 21483 9680 12593 6635 17089 28070 26914 54306 27924 5517 34026 16042 61848 2982 1378 8927 PC(42:7) 42042 39607 35030 43799 30359 27490 65765 67182 48698 123693 106445 49211 63275 37254 80063 3917 867 11643 PC(42:5) 19663 10941 16598 7065 18273 3451 16667 15989 16255 27658 14486 2378 6292 2995 11472 2284 743 9491 PC(45:12) 1631 10366 3372 3431 5209 1608 3822 1791 933 1786 2240 1999 6149 1742 643 681 548 1797 PC(O-16:0) 37080 39293 25768 42372 39205 38638 47886 45350 39171 29796 31022 38456 36069 35887 44342 30986 29167 31632 PC(19:2) 2947 2465 7619 2463 4293 2317 1730 2062 2693 2159 2749 2140 2325 2369 1484 395 954 1028 PC(26:2) 7604 5742 5608 5184 7210 6780 8343 8667 7843 8166 9732 6734 10881 10598 11774 2989 2917 2883 PC(O-44:7) 19025 8584 19097 6963 21299 3913 12807 16038 10519 7121 4977 737 1593 495 2863 1045 929 4165 PC(P-17:3_20:4) 115399 85338 112650 68305 98809 56204 76911 112859 79003 69374 64241 20711 26201 47910 70780 101268 70497 170056 PC(O-42:7) 18424 19589 23176 14536 40866 12680 26997 23401 25795 7936 14814 3108 1629 357 9332 588 436 967 PC(33:7) 5840 5519 1886 5263 3208 4363 3828 5861 6339 4974 2643 1901 1801 1407 2554 404 434 1 PC(O-43:13) 9842 6502 16063 7032 6767 11604 7915 22975 23830 14971 5396 4168 5003 6507 14754 10615 5222 381 PC(O-13:1_18:1) 61510 20357 62559 13196 57419 11528 31136 55083 25534 63423 64799 7749 24731 17290 58026 30893 19593 124707 PE(20:4_20:4) 75357 85852 60483 77112 96958 83188 132046 107041 77671 182728 125794 53226 123773 49576 132443 53827 50084 72094 PE(32:0) 8662 4317 6201 2623 4282 2122 1397 4489 4188 4562 4407 1293 3921 1755 5292 2916 2676 8465 PE(P-16:0_18:2) 22403 10980 23228 8051 23156 7498 12355 15527 10741 27603 27851 6492 22216 18057 34237 24589 15581 63104 PE(20:4_14:0) 40121 29671 31162 25023 36463 24206 28513 25886 31342 37777 23469 15374 11404 6865 15181 7940 7787 12084 PE(34:1) 9314 2977 9090 2316 8296 1673 3144 5898 5318 10611 6923 1195 3847 1102 7436 6639 4578 20865 PE(P-16:0_20:5) 64862 53437 77426 42957 48732 48642 58451 67169 60066 36435 27322 16286 39321 30575 58094 66985 27694 147905 PE(P-18:1_18:1) 79043 38321 69854 16983 70050 18272 31148 57862 35515 54670 51777 12898 98825 58365 73527 29354 29944 138234 PE(18:1_17:0) 24648 10760 23437 9030 18013 5688 13744 16288 14425 13293 18430 5442 16064 8787 24489 8729 7584 23626 PE(20:5_16:0) 197557 167242 157339 101516 223160 107715 187534 180957 174438 220362 183775 84552 156692 97834 197759 93365 68545 139698 PE(P-17:0_20:4) 42615 22562 48879 16417 34590 11528 24540 35549 25515 25693 25366 5659 40000 20617 81027 18977 14126 89065 PE(36:4) 6319 9316 26849 18183 20627 11866 16111 15340 13498 3085 8334 6678 20401 16528 30997 99141 139622 90191 PE(20:4_16:0) 62953 35637 55548 35642 45401 24615 45525 41941 42289 57517 41229 15235 18994 14468 32117 29336 25658 53192 PE(P-16:0_22:4) 1146792 516717 1339590 385546 1282650 240988 728001 986109 512326 1128031 850073 144888 847284 541496 1709480 439783 360791 1659717 PE(P-18:0_20:4) 56540 24863 66075 17687 47156 18851 34288 47797 34089 46161 37009 9629 37387 26310 74323 29111 21331 60123 PE(P-18:0_20:3) 77986 19687 70034 128 56557 48290 24924 46483 23879 162226 153815 39118 128152 146219 248304 12476 18007 25307 PE(20:4_18:0) 51052 21974 56176 16304 32122 9098 32379 35992 38829 59833 24239 8343 20616 8432 39732 12639 241392 41326 PE(P-18:0_22:5) 599991 317731 458424 148038 494108 102183 189559 383413 211874 286923 250745 34219 79144 40497 168145 123578 111046 392297 PE(P-16:0_18:1) 61222 20301 62270 13183 56854 10257 44459 54972 24340 63051 64514 7741 23937 17284 55549 30664 19590 115550 PE(P-17:0_18:1) 10777 8913 8598 6591 14070 1716 10660 14719 13171 13527 12253 4716 36905 29554 44367 6103 9665 21536 PE(P-16:0_20:4) 494133 303943 578281 272345 481163 184782 334415 448274 404097 340182 259033 116233 231571 140316 421436 21788 293155 1026644 PE(P-18:0_18:1) 13042 2962 12269 2156 7200 1778 3002 7284 5554 15523 13647 2674 55457 34413 78767 4409 4002 42137 PE(20:4_18:1) 5082 1374 2863 2478 5105 1954 9912 6052 4190 4820 2290 60 1608 7549 5312 25229 34541 12243 PE(O-18:1_22:4) 269644 85371 256638 52106 143118 34224 65289 118317 58221 189327 154513 16104 50353 22519 118336 34118 8 225675 PE(17:0) 2983 2637 950 897 2432 1719 681 2442 1448 1823 1798 802 1283 2352 481 137 437 1252 PE(20:4_17:0) 50558 41128 55674 37381 46419 23998 48052 55150 38734 64699 50149 16732 53789 25669 86351 14520 10959 30460 PE(P-18:1_22:6) 117553 82411 110414 75673 84467 60722 76591 108858 79643 70707 65516 20356 23728 47806 74580 102753 71492 174255 PE(22:6_18:1) 123694 86433 114226 76178 161799 93583 161016 171943 146224 228977 262439 88439 186245 152981 261353 133657 125283 173384 PE(22:7_18:0) 5503 40621 26045 12949 29692 39755 64992 46696 54908 18828 47528 26932 10724 33845 53189 14633 15539 10158 PE(18:0_22:6) 263517 155235 262024 134626 271572 106601 253243 274257 225161 282820 273007 59974 126900 93914 174055 112719 104249 182554 PE(16:1_8:0) 32627 24498 16732 16256 25517 19206 40256 37871 38906 23359 25864 22363 32444 29864 34839 1808 1908 1636 PE(25:1) 16288 9895 10502 8223 11559 9615 26419 21937 21889 13630 14104 12233 22855 22109 24278 1574 1323 1790 PE(34:3) 14871 13613 20039 8189 34208 11465 15861 14934 11464 4369 4526 2873 16759 14749 21907 925 1298 964 PE(18:3_16:0) 45316 31202 51655 24295 52009 22288 41419 45993 53897 43412 36240 19249 43216 25618 64618 35591 41938 43430 PE(22:6_16:0) 369811 274800 314783 247670 400765 221386 471830 455785 388218 454114 310246 129844 258534 141302 381642 270359 416181 733101 PE(O-40:6) 582140 349379 541833 195925 556061 136922 268734 382101 235924 364964 315907 48852 115806 54880 216528 129132 108502 411954 PE(P-18:0_22:4) 1088443 178737 788154 211428 708205 120179 375169 343823 146251 510653 541175 54139 228596 85952 166450 68139 48501 460794 PE(P-16:0_20:3) 35447 14829 74927 13443 75475 21396 20704 33730 19441 74338 78406 19981 60507 61087 155825 73599 40509 116254 PE(P-18:1_20:4) 1146681 779144 1235362 654696 1079897 502391 735393 962746 667795 791632 795593 260605 515913 290550 876688 526651 522543 1199713 PE(P-15:0_20:4) 975 2863 3748 1275 3334 1481 3070 3784 453 541 404 738 9273 5583 13510 10685 4994 30579 PE(O-18:1_20:3) 78006 19881 73314 132 56449 48664 25917 46658 23871 161494 154186 38627 128183 151569 248549 13380 18174 22882 PE(P-16:0_22:6) 116767 102108 131197 95432 60919 77198 110154 144366 112832 130666 116791 42825 82052 50074 114447 244093 116114 462000 PE(O-18:2_20:5) 28489 19506 31487 29415 37781 21592 24664 27795 21457 5954 3030 2628 5159 3058 5413 1213 1210 1253 PE(23:4) 14428 18341 10111 6982 18451 13082 4550 9728 13274 13263 12096 6021 14571 10472 3823 925 3072 9342 PE(19:1) 8295 6019 7455 5247 15455 4595 3134 5749 9487 3975 4917 3180 12312 5641 4317 593 2143 3826 PE(21:4) 25146 29059 33254 18069 25107 21688 9946 20066 28683 26220 19513 14424 21540 28213 6384 5131 17053 13632 PE(23:5) 4203 4712 2351 3139 5607 3333 1392 2576 5591 5139 3668 2029 6125 4320 1074 222 1311 2324 PE(16:1_16:0) 59891 37098 68367 25198 60040 15570 30738 42719 41584 60015 56082 20178 31588 19497 55422 14153 14783 24243 PG(20:6_18:0) 2357464 1274991 1890273 612042 1931917 1348101 1547151 1978951 1178215 1762886 1480747 325156 1530991 792493 1895827 603679 539270 2613034 PG(21:6_18:0) 34119 16303 38262 11129 32809 8338 19267 25643 21307 17409 21557 5358 25063 13655 37632 7022 5286 14547 PG(36:3) 3602 2278 3573 3134 4805 2593 8833 6792 5220 7188 5418 3085 11352 5741 21235 956 10 1118 PG(20:6_18:2) 23059 20177 18916 15136 34868 21806 29079 33797 23198 21185 17533 8458 34891 26019 38478 40840 35316 51390 PG(22:4_18:2) 18867 30438 14680 1038 9971 12516 7894 19337 9535 7584 24034 3215 9144 7891 18197 479 480 712 PG(22:6_18:0) 426505 148404 329347 36253 225694 39634 140254 239779 142817 108460 81945 43543 57211 51185 55853 33635 38719 233045 PG(20:6_22:6) 5513 7529 3664 7477 6635 11359 8097 10618 4161 12844 10647 4658 3599 5415 6344 41377 30613 44646 PG(20:6_22:4) 30632 32112 14446 17955 25515 25091 30181 27951 19835 67151 45294 23902 18192 10519 30213 9589 5650 10550 PG(22:5_20:3) 13045 19830 7988 14271 4644 6634 6622 13231 7386 12281 27528 13945 5158 4332 8880 1 0 3 PG(O-30:1_20:4) 5841 7024 6759 6907 7174 6842 6557 7730 7902 6376 7158 6435 5849 6935 7021 7311 7583 6459 PG(20:6_22:3) 50842 37104 30816 21151 53887 28581 37767 42126 23257 148819 131900 48206 51794 29133 64874 3919 3868 5758 PG(20:6_16:0) 43849 29416 35952 17125 49965 26057 38872 41857 25885 36883 26850 8218 21553 12550 24639 33460 34608 60775 PG(20:6_20:2) 244826 136815 223318 104652 233390 77940 138583 197861 88112 151350 155386 43438 97563 75737 85558 200979 143078 406121 PG(36:4) 6719 7457 5155 6358 8111 5880 11036 10010 6382 3551 8571 3326 23714 16171 25124 0 0 0 PG(20:6_17:0) 63007 28889 42566 19240 57142 18837 42863 49116 33175 27305 24425 7135 57800 31007 71372 14339 13131 13012 PG(O-30:3_20:4) 4832 4617 4681 3710 4382 4209 4498 3648 4081 4465 4107 4245 4128 3539 4268 4636 3832 4581 PG(20:3_18:2) 15092 22060 19223 19505 21681 17628 31191 25391 22269 23551 30044 18330 36691 31206 42213 1838 1862 2673 PG(20:6_18:1) 420136 244489 306733 148719 448288 183875 314611 397997 264387 225684 222846 79162 347025 223356 409107 190671 200650 308149 PI(34:2) 1055 722 1280 395 1268 317 990 1155 1090 1234 819 1 155 164 661 5337 3941 10936 PI(20:4_16:0) 16098 14155 15417 12310 15399 13703 16424 20076 11619 22917 16404 7016 9345 5363 13606 28200 18554 61748 PI(20:4_18:0) 89815 44381 112800 30382 71901 20395 67520 77562 54962 87378 66911 15622 45424 22311 88647 21644 10019 62796 PI(20:4_18:1) 41968 19604 45032 20836 59718 21852 36600 37583 33131 14901 12702 3854 13592 9176 16597 12772 9473 30132 PI(18:1_18:0) 28556 15133 33257 10975 26258 7909 21935 21497 14964 35127 25311 6896 14596 8052 25231 5501 2988 20234 PI(18:2_18:0) 29572 23142 37823 17342 48795 18300 25605 24500 24282 5964 4406 2473 8084 6219 7466 3613 2969 7751 PI(O-43:9) 35486 30271 27051 33177 34356 42231 39019 34071 38371 44272 30901 29818 35835 23249 34773 32454 33093 34889 PS(18:0_18:2) 142912 112250 99780 88704 164472 116530 128928 124952 95694 89243 93097 53024 108254 99615 104513 25736 29114 60609 PS(18:0_20:3) 113758 110418 349731 20243 127623 16429 58873 120724 50315 108913 121275 15477 185760 125641 294359 28117 59206 89854 PS(18:0_20:4) 2323327 1248780 1854711 603955 1922167 601928 1482992 1950056 1160302 1754163 1453770 323148 1511278 786283 1858957 600232 529336 1187824 PS(18:0_22:5) 469312 217863 366606 136796 307659 95506 208347 313250 203580 245422 185505 39937 69622 40072 100198 112928 102135 317555 PS(18:1_20:4) 388405 246325 326035 147187 447255 183621 314150 389148 279788 226025 220429 79037 325983 223194 406139 191186 201813 307622 PS(20:4_20:4) 36976 44056 24279 30655 46654 63255 55570 57488 24285 87259 60951 28203 66043 49055 53390 39333 43021 44739 PS(20:4_22:6) 5564 6935 3659 7462 6832 11326 8112 10606 4294 12857 9911 4612 3622 9230 6472 43493 30612 45378 PS(20:4_18:0) 33408 25879 35873 9164 12406 742831 60706 17277 17923 11623 29165 1982 19187 5357 34935 3676 9801 1405772 PS(38:4) 54434 25228 31309 15165 42203 13937 37176 24135 22319 142 23069 6354 21898 19054 28308 12886 11493 18163 PS(34:1) 16861 5256 23432 3913 17809 3043 6771 11361 9458 7940 5829 1016 6424 2727 12091 6002 8363 16490 PS(20:4_16:0) 40602 28158 32751 17419 44673 23528 38879 44912 25487 38719 25311 7715 21268 12548 21675 33442 31072 53941 PS(36:2) 33705 10532 61370 8150 42154 6628 16371 21501 24989 19657 15276 5220 46427 27600 101219 0 1 0 PS(20:4_18:2) 29591 20151 23538 14483 35372 21776 28700 34729 23206 21174 17544 8290 31864 24558 35839 38048 33942 49711 PS(20:5_18:0) 46697 38639 23306 26596 73304 41683 64983 38657 34824 36975 29049 22283 26964 25173 35854 9511 10907 9600 PS(22:4_18:0) 457942 148510 329908 38861 230772 40283 140704 239764 142969 108238 81796 40657 56793 54991 56853 33531 38661 233025 PS(O-38:4) 138874 137470 115538 99472 199381 118659 200285 179255 176025 144619 103125 91280 143955 118824 142145 18364 16665 16988 PS(20:4_20:2) 258977 119840 223293 104688 67440 22831 136699 194939 123846 162965 149241 12521 57655 59449 112878 217565 122869 405012 PS(20:4_17:0) 59438 27623 40682 19256 58723 18153 41884 51751 33416 61116 24444 7128 55892 34226 67716 14158 13139 13019 PS(18:3_18:0) 17710 6979 29828 6709 24601 5914 14296 16205 16936 8844 8619 6194 25070 18719 41411 12601 22270 22614 PS(20:4_22:3) 50871 41153 29921 21145 53998 28535 41143 42058 23203 135910 132108 48207 51904 28835 64792 3926 3878 5761 PS(42:9) 13509 11362 7370 13286 11411 16840 13591 13945 8526 21017 16182 8303 5297 3085 5247 6676 13840 18017 PS(34:2) 15036 5862 15467 3917 16439 4385 8581 10348 10096 8502 7101 1747 11644 6928 16009 3465 4584 4454 PS(40:7) 35580 68448 70526 48077 51491 46266 66379 73048 66176 55441 68219 32717 83239 59103 74071 45835 43418 55731 PS(4:0_18:0) 6586 8381 4613 5610 9823 6464 9731 7108 4712 8231 5915 5139 7056 5120 4524 1983 2184 2512 PS(10:0_18:0) 7762 6946 8342 8436 7914 9006 9051 9812 13880 8618 11943 9045 10041 9865 9777 5722 6682 5975 PS(21:4_18:0) 36868 16651 38681 11594 32822 8345 19663 25439 21326 21815 20242 5361 26709 13688 40257 7444 5274 14567 SM(d31:1) 16463 12762 10301 10426 15138 11706 19007 20889 17268 18384 18567 9272 33453 25143 35073 16315 14247 31609 SM(d32:1) 665112 505351 482478 373690 566859 376729 499136 700935 534299 831928 755891 293866 985809 615772 1204268 558448 481224 1154192 SM(d34:1) 4331836 1854710 3315983 1454999 3233418 1208022 2844487 3574748 2252204 6224690 4400023 812432 3322203 1679415 5653739 1919764 1383252 5487811 SM(d35:2) 29018 14284 19557 12229 22663 9340 17941 29013 17422 28396 20667 5903 46251 31644 72018 18599 12246 38524 SM(d36:1) 161236 67764 144114 42545 127836 42896 121821 171924 105985 265420 257757 28024 250479 112507 385924 64763 36108 335459 SM(d40:1) 381968 212896 347040 156998 359187 117877 268750 358624 239183 451254 351691 64330 70113 30150 140979 67776 35676 190176 SM(d42:1) 97932 40098 92702 29787 71859 16758 39449 72464 42968 210519 135449 31031 12286 3500 27575 5941 3753 37412 SM(d42:2) 432454 118579 389571 79818 254631 50670 125633 215392 137839 671383 532633 60167 62679 22059 138720 101742 50135 781155 SM(d43:2) 268 386 918 406 295 1 520 629 203 2434 2280 1 132 1 633 791 365 4776 SM(d14:0_20:1) 47815 17542 27401 13535 30853 14298 30432 34485 19121 46651 56022 9231 35716 20059 66918 17733 15682 70901 SM(d42:3) 807210 337882 903853 198546 716200 129177 397176 618065 322548 1057678 773940 81131 123635 54087 324620 245671 144268 1008845 SM(d42:4) 358827 197134 327750 144096 342319 121841 269843 306670 231366 406807 308236 59608 59811 24784 126866 63454 28925 149699 SM(d38:1) 20106 5850 17952 4422 12267 1954 13581 15369 12659 49351 44396 3270 19903 6978 41877 6128 1959 43467 SM(d30:1) 29861 26491 18526 23267 26984 23864 25984 31784 26132 20222 20768 14351 31400 24865 31839 24635 26486 42518 SM(d33:1) 306741 169124 215300 102321 256649 102895 203509 273654 158182 446743 355711 77419 596951 273872 805049 212334 121935 494092 SM(d34:2) 768876 510876 576122 383987 871001 489872 676907 805697 657232 731307 639053 323158 1114923 867332 1193158 735962 659686 1010354 SM(d36:3) 37136 24988 27327 15537 43530 15232 23386 35012 24480 27229 27469 8607 39641 26669 44388 21336 21093 35383 SM(d36:2) 457651 270225 412311 194027 472180 160258 360430 455641 321698 418479 380501 107108 504854 292634 751991 277680 265492 742642 SM(d36:0) 7627 1127 3561 856 901 1149 775 3013 897 10600 10996 1229 4881 975 9586 834 805 1182 SM(d38:2) 57755 39898 57609 25565 61477 21137 57126 74174 54659 113436 113115 17775 110080 64911 165620 67109 43636 219158 SM(d39:1) 1 1 1 1 1 1 1 1 321 572 782 125 555 1 1 252 212 5368 SM(d40:3) 129483 66105 102451 36173 95831 36040 100032 110984 82941 113848 86073 15402 31139 19112 57009 32110 20991 65681 SM(d40:2) 202715 66041 214818 36728 136617 25657 115117 167186 92610 361991 327696 7649 63557 24986 171144 80486 39528 389543 SM(d44:2) 5989 1124 5248 1169 2526 545 1182 1714 1411 10585 8671 1293 331 128 1749 625 310 7330 SM(d44:1) 137 1 1 1 1 1 1 866 1 1 221 1075 1 1 1 1 1 582 SM(d33:2) 14358 11081 10235 8200 13906 9988 16708 20138 14762 15486 13917 7746 34526 28757 42234 12495 11998 18646 SM(d32:0) 29389 16917 19838 10892 21100 9379 15102 20233 17708 37377 32856 10083 39260 21519 55391 12840 10549 30650 SM(d38:3) 15080 10096 11667 7104 17722 7857 11398 9784 8756 12002 13334 2475 9039 4450 17805 8070 7946 19128 SM(d41:1) 719 1 468 229 280 1 1 683 346 1966 1508 503 423 241 499 483 126 4005 SM(d44:5) 62543 41625 51488 23168 62192 17744 36140 48286 42228 162874 117200 23043 12377 1935 26220 12514 6407 65786 SM(d44:3) 26511 8027 26887 5141 16586 2972 8080 13078 5752 44087 33418 3852 3002 1212 9984 3054 2176 23031 SM(d42:5) 3566 4004 4088 4044 3373 2562 3351 9217 7925 14479 17767 1976 5 3059 9313 4714 0 6425 SM(d44:4) 7529 4324 6201 2582 6430 1693 3619 4530 3974 13643 9233 2639 593 193 874 470 1 1340 SM(d44:6) 36694 29322 55106 21196 22154 14503 24775 39993 31251 30056 41445 9501 16019 5633 38925 26222 13103 20923 SM(d34:4) 21669 15659 17459 16328 0 15657 1 23752 19875 0 26709 17803 119 29221 25720 11095 26121 1 SPH(d20:0) 30970 35106 46182 88428 78435 23645 16303 48129 111084 95508 21725 16700 9589 8981 10446 9107 9246 5671 SPH(d16:1) 7776 9799 7911 3882 10989 6313 12619 10155 8349 20160 24896 12316 14428 9906 20072 9954 6966 6615 SPH(d19:0) 7552 7305 6111 6221 9483 6942 20509 5659 7622 10501 13748 10205 9388 8356 10949 2062 1921 2140 SPH(d17:0) 15433 14683 11280 13650 20929 20979 28552 21671 17518 60539 58617 40251 38087 28620 45799 10293 4757 6453 SPH(d18:1) 28682 16456 18831 14191 26078 16117 14476 21548 24780 17594 30135 29823 43363 39477 76296 9529 1903 9657 SPH(d16:0) 20142 20166 19805 17893 30221 19268 32969 45491 36424 43026 70387 57158 67961 63336 78085 7954 7466 8147 TG(14:0_14:0_16:0) 6827 7449 7081 7141 6756 7524 7311 6689 6008 7067 6669 7704 6942 8050 8873 7005 7500 8265 TG(14:0_15:0_16:0) 8917 8831 7704 8661 8219 8921 8457 9151 8484 8226 8628 9235 9032 8936 9474 8400 8863 8885 TG(14:0_16:0_16:0) 12857 12812 13175 12585 12690 15277 12151 14625 12040 12723 12032 15500 15593 15172 17197 12456 12556 14362 TG(15:0_16:0_16:0) 12031 12110 11792 12392 10453 11304 9572 10520 10130 10203 10051 13085 10567 9384 13628 10513 12321 11905 TG(16:0_16:0_16:0) 13495 14615 14137 13134 13326 17000 13033 13355 13241 13131 14026 15641 13618 12025 15074 12381 15174 15287 TG(16:0_16:0_18:0) 7314 5790 6056 6321 7201 5603 5817 5637 6909 6304 6373 6447 5552 5773 6519 5819 5236 5481 TG(16:0_18:0_18:0) 4230 4178 4066 4522 3871 3777 3036 4374 2707 4456 3957 4526 3996 3398 3669 4495 3443 4172 TG(51:14_9:0) 4674 4896 4764 3779 4184 4400 4228 4438 3800 4472 4494 4382 4559 5632 4794 4725 4066 4341 TG(20:4_20:4_20:4) 6883 5349 6759 6904 7173 5351 6270 7731 6666 6076 5445 6865 5202 6877 6822 6870 6914 6837 TG(17:0_16:0_16:0) 5590 4767 4523 4918 5142 5055 5728 4081 5505 5445 6014 5766 4720 5617 4010 4743 5733 5144 TG(16:0_18:1_18:1) 2926 2094 2082 2008 2421 2914 2366 2558 2413 2231 2695 2397 3081 2129 2851 2555 2739 2430 TG(14:0_14:0_14:0) 2960 3879 4071 3173 3247 2849 2949 3622 3126 3277 3346 3485 2594 3263 3512 3892 3262 3410 TG(18:0_18:0_18:0) 2703 3090 3147 3075 2880 3433 3355 2801 2751 3003 2643 3101 2556 2867 3319 2354 2880 3273 TG(13:0_15:0_15:0) 3231 3365 3112 3343 3113 3308 3189 3401 3390 2794 4022 3513 3180 3342 4063 3926 3391 4176 TG(21:1) 1767 929 1938 1057 1429 1512 1481 1184 1557 2666 1752 1561 1939 1398 1749 1613 1370 1709 TG(19:2) 68005 79949 41591 87744 179809 182844 422839 394769 206506 496641 248924 151958 198345 95598 317949 642778 631420 695369 TG(28:4_26:6_26:6) 4073 2737 2787 2690 5421 2872 5690 4693 4628 5120 5134 2778 6065 5982 6191 358 1 458 TG(18:1_18:1_18:1) 2167 2008 1993 1971 2315 1501 2417 2760 2329 2354 2684 1640 2336 2344 1936 2126 2307 2355 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name Lipid Class Polarity m/z RT AcCa(14:0) AcCa 5MM+ 372.311 1.232 AcCa(18:1) AcCa 5MM+ 426.358 1.702 AcCa(18:0) AcCa 5MM+ 428.373 1.929 AcCa(26:0) AcCa 5MM+ 540.499 2.799 AcCa(2:0) AcCa 5MM+ 204.123 0.240 AcCa(16:0) AcCa 5MM+ 400.342 1.618 AcCa(17:0) AcCa 5MM+ 414.358 1.716 AcCa(20:3) AcCa 5MM+ 450.358 1.611 AcCa(20:2) AcCa 5MM+ 452.373 1.791 AcCa(22:5) AcCa 5MM+ 474.358 1.554 AcCa(18:2) AcCa 5MM+ 424.342 1.480 AcCa(15:0) AcCa 5MM+ 386.326 1.445 AcCa(12:0) AcCa 5MM+ 344.280 0.762 AcCa(4:0) AcCa 5MM+ 232.154 0.259 AcCa(17:1) AcCa 5MM+ 412.342 1.550 AcCa(16:1) AcCa 5MM+ 398.326 1.357 Cer(d18:1_16:0) Cer 5MM+ 520.509 3.061 Cer(d18:2_24:1) Cer 5MM+ 628.603 3.297 Cer(d18:2_16:0) Cer 5MM+ 536.504 2.962 Cer(d18:2_18:0) Cer 5MM+ 546.524 3.080 Cer(d18:1_22:2) Cer 5MM+ 618.582 3.206 Cer(d16:1_16:0) Cer 5MM+ 492.478 2.928 Cer(d18:2_22:0) Cer 5MM+ 620.598 3.284 Cer(d18:2_23:0) Cer 5MM+ 634.613 3.307 Cer(d18:0_24:0) Cer 5MM+ 634.650 3.424 Cer(d18:1_24:1) Cer 5MM+ 648.629 3.346 Cer(d18:1_18:0) Cer 5MM+ 566.551 3.150 Cer(d18:1_24:0) Cer 5MM+ 650.645 3.121 Ch Ch 5MM+ 369.352 2.630 DG(16:0_18:0) DG 5MM+ 614.572 3.337 DG(18:1_18:2) DG 5MM+ 636.556 3.206 DG(18:1_18:1) DG 5MM+ 638.572 3.282 DG(16:0_18:1) DG 5MM+ 612.556 3.263 DG(18:0_20:4) DG 5MM+ 662.572 3.269 DG(O-26:4) DG 5MM+ 463.378 2.273 DG(16:0_18:2) DG 5MM+ 610.541 3.196 DG(16:0_20:4) DG 5MM+ 634.541 3.166 Hex1Cer(d18:1_16:0) Hex1Cer 5MM+ 700.572 3.026 LPC(16:0) LPC 5MM+ 496.340 1.635 LPC(17:0) LPC 5MM+ 510.355 1.750 LPC(18:0) LPC 5MM+ 524.371 1.895 LPC(O-17:0) LPC 5MM+ 496.376 1.930 LPC(O-17:1) LPC 5MM+ 494.361 1.924 LPC(20:3) LPC 5MM+ 546.355 1.623 LPC(P-18:0) LPC 5MM+ 508.376 1.898 LPC(30:0) LPC 5MM+ 692.559 2.960 LPC(20:0) LPC 5MM+ 552.402 2.046 LPC(P-21:5) LPC 5MM+ 562.327 2.004 LPC(24:1) LPC 5MM+ 606.449 2.465 LPC(15:1) LPC 5MM+ 480.308 1.774 LPC(P-16:0) LPC 5MM+ 480.345 1.806 LPC(P-15:0) LPC 5MM+ 466.329 1.594 LPC(19:0) LPC 5MM+ 538.387 2.102 LPC(22:4) LPC 5MM+ 572.371 1.808 LPC(23:1) LPC 5MM+ 592.434 2.333 LPC(P-17:0) LPC 5MM+ 494.361 1.915 LPC(P-18:1) LPC 5MM+ 506.361 1.867 LPC(O-18:1) LPC 5MM+ 508.376 2.105 LPC(20:2) LPC 5MM+ 548.371 1.857 LPC(O-22:0) LPC 5MM+ 566.454 2.575 LPC(14:0) LPC 5MM+ 468.308 1.345 LPC(15:0) LPC 5MM+ 482.324 1.533 LPC(O-16:0) LPC 5MM+ 482.361 1.834 LPC(16:1) LPC 5MM+ 494.324 1.455 LPC(O-18:0) LPC 5MM+ 510.392 2.114 LPC(18:2) LPC 5MM+ 520.340 1.502 LPC(20:4) LPC 5MM+ 544.340 1.496 LPC(18:1) LPC 5MM+ 522.355 1.769 LPE(22:6) LPE 5MM+ 526.293 1.512 LPE(16:1) LPE 5MM+ 452.277 1.486 LPE(18:2) LPE 5MM+ 478.293 1.603 LPE(20:5) LPE 5MM+ 500.277 1.337 LPG(18:2) LPG 5MM+ 509.287 1.328 LPS(20:4) LPS 5MM+ 546.283 1.234 PA(O-20:2_20:4) PA 5MM+ 752.559 3.781 PA(O-20:2_22:4) PA 5MM+ 780.590 3.327 PA(22:5_14:0) PA 5MM+ 712.491 2.815 PA(22:5_16:0) PA 5MM+ 740.522 3.033 PA(O-18:2_20:5) PA 5MM+ 722.512 2.721 PA(30:1_19:3) PA 5MM+ 896.710 3.318 PA(O-19:2_20:4) PA 5MM+ 738.543 3.070 PA(22:6_18:0) PA 5MM+ 766.538 2.700 PA(O-20:3_18:2) PA 5MM+ 726.543 3.040 PA(O-20:2_22:5) PA 5MM+ 778.575 3.121 PA(22:6_16:0) PA 5MM+ 738.507 2.562 PA(O-20:3_20:5) PA 5MM+ 748.528 2.680 PA(O-18:2_22:5) PA 5MM+ 750.543 3.035 PA(21:2_4:0) PA 5MM+ 564.366 1.913 PA(O-20:3_16:1) PA 5MM+ 700.528 3.027 PC(18:1_16:0) PC 5MM+ 782.567 3.008 PC(34:1) PC 5MM+ 760.585 3.114 PC(34:0) PC 5MM+ 762.601 3.093 PC(36:4) PC 5MM+ 782.569 2.844 PC(14:1_22:3) PC 5MM+ 782.569 3.144 PC(36:2) PC 5MM+ 786.601 3.040 PC(32:0) PC 5MM+ 734.569 2.984 PC(38:4) PC 5MM+ 810.601 3.036 PC(36:1) PC 5MM+ 788.616 3.121 PC(28:0) PC 5MM+ 678.507 2.700 PC(30:1) PC 5MM+ 704.522 2.746 PC(30:0) PC 5MM+ 706.538 2.853 PC(31:0) PC 5MM+ 720.554 2.913 PC(O-32:0) PC 5MM+ 720.590 3.066 PC(32:2) PC 5MM+ 730.538 2.788 PC(O-15:1_18:1) PC 5MM+ 730.575 3.202 PC(33:3) PC 5MM+ 742.538 2.421 PC(17:3_16:0) PC 5MM+ 742.538 2.973 PC(O-34:2) PC 5MM+ 744.590 2.996 PC(O-34:1) PC 5MM+ 746.606 3.082 PC(P-15:2_20:4) PC 5MM+ 748.528 2.975 PC(O-34:0) PC 5MM+ 748.621 3.178 PC(O-35:6) PC 5MM+ 750.543 2.860 PC(O-13:1_22:5) PC 5MM+ 750.543 3.038 PC(34:4) PC 5MM+ 754.538 2.778 PC(34:2) PC 5MM+ 758.569 2.554 PC(35:6) PC 5MM+ 764.522 2.571 PC(O-36:6) PC 5MM+ 764.559 2.710 PC(O-14:1_22:5) PC 5MM+ 764.559 2.893 PC(35:5) PC 5MM+ 766.538 2.754 PC(35:4) PC 5MM+ 768.554 2.848 PC(19:4_16:0) PC 5MM+ 768.554 3.063 PC(O-36:4) PC 5MM+ 768.590 2.999 PC(O-14:1_22:3) PC 5MM+ 768.590 3.129 PC(35:2) PC 5MM+ 772.585 2.976 PC(O-15:2_22:6) PC 5MM+ 774.543 2.893 PC(O-37:8) PC 5MM+ 774.543 3.123 PC(36:6) PC 5MM+ 778.538 2.691 PC(O-35:2) PC 5MM+ 780.588 3.290 PC(O-37:4) PC 5MM+ 782.606 3.038 PC(O-15:1_22:3) PC 5MM+ 782.606 3.202 PC(36:3) PC 5MM+ 784.585 2.591 PC(O-38:6) PC 5MM+ 792.590 2.945 PC(O-38:5) PC 5MM+ 794.606 3.010 PC(37:2) PC 5MM+ 800.616 3.089 PC(22:6_16:1) PC 5MM+ 804.554 2.791 PC(O-39:7) PC 5MM+ 804.590 3.133 PC(22:6_16:0) PC 5MM+ 828.551 2.889 PC(38:6) PC 5MM+ 806.569 2.963 PC(O-17:1_22:4) PC 5MM+ 808.621 3.069 PC(38:2) PC 5MM+ 814.632 3.144 PC(O-40:4) PC 5MM+ 824.653 3.179 PC(17:3_23:5) PC 5MM+ 830.569 2.825 PC(40:8) PC 5MM+ 830.569 2.938 PC(40:4) PC 5MM+ 838.632 3.072 PC(O-42:2) PC 5MM+ 856.715 3.377 PC(42:2) PC 5MM+ 870.695 3.296 PC(44:2) PC 5MM+ 898.726 3.401 PC(O-32:1) PC 5MM+ 718.575 2.860 PC(O-13:1_20:5) PC 5MM+ 722.512 2.940 PC(32:1) PC 5MM+ 732.554 2.890 PC(33:2) PC 5MM+ 744.554 2.352 PC(O-35:7) PC 5MM+ 748.528 2.655 PC(O-15:2_20:5) PC 5MM+ 748.528 2.980 PC(35:1) PC 5MM+ 774.601 3.059 PC(O-15:2_22:3) PC 5MM+ 780.590 3.167 PC(37:8) PC 5MM+ 788.522 2.866 PC(37:6) PC 5MM+ 792.554 2.801 PC(37:5) PC 5MM+ 794.569 2.886 PC(19:4_18:1) PC 5MM+ 794.569 3.073 PC(37:4) PC 5MM+ 796.585 2.797 PC(O-38:4) PC 5MM+ 796.621 3.104 PC(O-15:1_22:6) PC 5MM+ 776.559 3.076 PC(37:3) PC 5MM+ 798.601 3.205 PC(38:5) PC 5MM+ 808.585 2.940 PC(39:6) PC 5MM+ 820.585 3.104 PC(40:7) PC 5MM+ 832.585 2.887 PC(22:7_18:0) PC 5MM+ 832.585 3.031 PC(40:5) PC 5MM+ 836.616 3.053 PC(40:1) PC 5MM+ 844.679 3.294 PC(O-42:4) PC 5MM+ 852.684 3.285 PC(O-44:6) PC 5MM+ 876.684 3.229 PC(O-44:4) PC 5MM+ 880.715 3.382 PC(33:1) PC 5MM+ 746.569 2.951 PC(O-36:1) PC 5MM+ 774.637 3.120 PC(O-39:8) PC 5MM+ 802.575 3.085 PC(O-36:2) PC 5MM+ 772.621 3.056 PC(O-36:3) PC 5MM+ 770.606 3.034 PC(37:1) PC 5MM+ 802.632 3.167 PC(O-40:6) PC 5MM+ 820.621 3.009 PC(O-42:1) PC 5MM+ 858.731 3.449 PC(20:1) PC 5MM+ 564.366 2.133 PC(21:4) PC 5MM+ 572.335 1.553 PC(24:0) PC 5MM+ 622.444 2.381 PC(25:0) PC 5MM+ 636.460 2.484 PC(26:0) PC 5MM+ 650.476 2.568 PC(O-13:1_20:4) PC 5MM+ 724.528 3.013 PC(32:3) PC 5MM+ 728.522 2.754 PC(34:5) PC 5MM+ 752.522 2.679 PC(18:3_16:0) PC 5MM+ 756.554 2.470 PC(34:3) PC 5MM+ 756.554 2.625 PC(O-36:5) PC 5MM+ 766.575 2.917 PC(37:7) PC 5MM+ 790.538 2.941 PC(O-40:7) PC 5MM+ 818.606 2.984 PC(40:6) PC 5MM+ 834.601 3.014 PC(40:3) PC 5MM+ 840.648 3.165 PC(O-42:5) PC 5MM+ 850.668 3.215 PC(42:8) PC 5MM+ 858.601 2.924 PC(42:6) PC 5MM+ 862.632 3.055 PC(O-44:5) PC 5MM+ 878.700 3.295 PC(20:0) PC 5MM+ 566.382 1.987 PC(O-37:6) PC 5MM+ 778.575 3.055 PC(O-39:10) PC 5MM+ 820.525 2.914 PC(O-40:3) PC 5MM+ 826.668 3.249 PC(O-42:6) PC 5MM+ 848.653 3.128 PC(O-43:12) PC 5MM+ 850.575 1.861 PC(42:10) PC 5MM+ 854.569 2.801 PC(42:4) PC 5MM+ 866.663 3.179 PC(44:8) PC 5MM+ 886.632 3.016 PC(O-49:12) PC 5MM+ 934.668 2.516 PC(22:1) PC 5MM+ 592.397 2.170 PC(23:0) PC 5MM+ 608.429 2.281 PC(O-16:2_18:2) PC 5MM+ 740.559 2.883 PC(O-35:4) PC 5MM+ 754.575 2.892 PC(35:7) PC 5MM+ 762.507 2.805 PC(38:3) PC 5MM+ 812.616 3.067 PC(O-40:5) PC 5MM+ 822.637 3.115 PC(22:0) PC 5MM+ 594.413 2.232 PC(O-45:13) PC 5MM+ 876.590 2.077 PC(26:1) PC 5MM+ 648.460 2.420 PC(31:1) PC 5MM+ 718.538 2.838 PC(O-33:1) PC 5MM+ 732.590 3.034 PC(33:4) PC 5MM+ 740.522 2.898 PC(O-18:4_17:1) PC 5MM+ 752.559 2.889 PC(35:3) PC 5MM+ 770.569 2.893 PC(36:5) PC 5MM+ 780.554 2.756 PC(38:1) PC 5MM+ 816.648 3.140 PC(39:5) PC 5MM+ 822.601 2.993 PC(38:8) PC 5MM+ 802.538 2.834 PC(42:12) PC 5MM+ 850.538 1.795 PC(P-33:5) PC 5MM+ 722.512 2.943 PC(O-35:1) PC 5MM+ 760.621 3.129 PC(42:9) PC 5MM+ 856.585 2.887 PC(O-45:12) PC 5MM+ 878.606 2.194 PC(O-38:3) PC 5MM+ 798.637 3.153 PC(O-39:9) PC 5MM+ 800.559 3.126 PC(29:1) PC 5MM+ 690.507 2.941 PC(P-9:0_20:1) PC 5MM+ 696.494 2.891 PC(31:2) PC 5MM+ 716.522 2.959 PC(39:4) PC 5MM+ 824.616 3.070 PC(42:7) PC 5MM+ 860.616 2.984 PC(42:5) PC 5MM+ 864.648 3.134 PC(45:12) PC 5MM+ 892.585 2.315 PC(O-16:0) PC 5MM+ 518.322 1.704 PC(19:2) PC 5MM+ 548.335 1.569 PC(26:2) PC 5MM+ 646.444 2.269 PC(O-44:7) PC 5MM+ 874.668 3.176 PC(P-17:3_20:4) PC 5MM+ 774.543 3.007 PC(O-42:7) PC 5MM+ 846.637 3.077 PC(33:7) PC 5MM+ 734.476 2.704 PC(O-43:13) PC 5MM+ 848.559 3.031 PC(O-13:1_18:1) PC 5MM+ 702.543 3.105 PE(20:4_20:4) PE 5MM+ 788.522 2.864 PE(32:0) PE 5MM+ 692.522 3.011 PE(P-16:0_18:2) PE 5MM+ 700.528 3.027 PE(20:4_14:0) PE 5MM+ 712.491 2.817 PE(34:1) PE 5MM+ 718.538 3.167 PE(P-16:0_20:5) PE 5MM+ 722.512 2.664 PE(P-18:1_18:1) PE 5MM+ 728.559 3.124 PE(18:1_17:0) PE 5MM+ 732.554 3.089 PE(20:5_16:0) PE 5MM+ 738.507 2.561 PE(P-17:0_20:4) PE 5MM+ 738.543 3.066 PE(36:4) PE 5MM+ 740.522 2.886 PE(20:4_16:0) PE 5MM+ 740.522 3.035 PE(P-16:0_22:4) PE 5MM+ 752.559 3.044 PE(P-18:0_20:4) PE 5MM+ 752.559 3.247 PE(P-18:0_20:3) PE 5MM+ 754.575 3.133 PE(20:4_18:0) PE 5MM+ 768.554 3.147 PE(P-18:0_22:5) PE 5MM+ 778.575 3.138 PE(P-16:0_18:1) PE 5MM+ 702.543 3.101 PE(P-17:0_18:1) PE 5MM+ 716.559 2.958 PE(P-16:0_20:4) PE 5MM+ 724.528 3.015 PE(P-18:0_18:1) PE 5MM+ 730.575 2.982 PE(20:4_18:1) PE 5MM+ 766.538 3.068 PE(O-18:1_22:4) PE 5MM+ 780.590 3.251 PE(17:0) PE 5MM+ 482.288 1.750 PE(20:4_17:0) PE 5MM+ 754.538 3.005 PE(P-18:1_22:6) PE 5MM+ 774.543 3.014 PE(22:6_18:1) PE 5MM+ 790.538 2.913 PE(22:7_18:0) PE 5MM+ 790.538 3.062 PE(18:0_22:6) PE 5MM+ 792.554 3.011 PE(16:1_8:0) PE 5MM+ 578.382 2.251 PE(25:1) PE 5MM+ 592.397 2.378 PE(34:3) PE 5MM+ 714.507 2.552 PE(18:3_16:0) PE 5MM+ 714.507 2.893 PE(22:6_16:0) PE 5MM+ 764.522 2.923 PE(O-40:6) PE 5MM+ 778.575 3.062 PE(P-18:0_22:4) PE 5MM+ 780.590 3.127 PE(P-16:0_20:3) PE 5MM+ 726.543 3.027 PE(P-18:1_20:4) PE 5MM+ 750.543 3.036 PE(P-15:0_20:4) PE 5MM+ 710.512 2.941 PE(O-18:1_20:3) PE 5MM+ 754.575 3.120 PE(P-16:0_22:6) PE 5MM+ 748.528 2.971 PE(O-18:2_20:5) PE 5MM+ 748.528 2.655 PE(23:4) PE 5MM+ 558.319 1.853 PE(19:1) PE 5MM+ 508.303 1.835 PE(21:4) PE 5MM+ 530.288 1.611 PE(23:5) PE 5MM+ 556.303 1.684 PE(16:1_16:0) PE 5MM+ 690.507 2.937 PG(20:6_18:0) PG 5MM+ 812.544 2.793 PG(21:6_18:0) PG 5MM+ 826.559 2.816 PG(36:3) PG 5MM+ 790.559 2.709 PG(20:6_18:2) PG 5MM+ 808.512 2.592 PG(22:4_18:2) PG 5MM+ 840.575 2.665 PG(22:6_18:0) PG 5MM+ 840.575 2.887 PG(20:6_22:6) PG 5MM+ 856.512 2.563 PG(20:6_22:4) PG 5MM+ 860.544 2.677 PG(22:5_20:3) PG 5MM+ 864.575 2.660 PG(O-30:1_20:4) PG 5MM+ 968.768 3.733 PG(20:6_22:3) PG 5MM+ 862.559 2.768 PG(20:6_16:0) PG 5MM+ 784.512 2.664 PG(20:6_20:2) PG 5MM+ 836.544 2.786 PG(36:4) PG 5MM+ 788.544 2.630 PG(20:6_17:0) PG 5MM+ 798.528 2.735 PG(O-30:3_20:4) PG 5MM+ 964.736 3.642 PG(20:3_18:2) PG 5MM+ 814.559 2.643 PG(20:6_18:1) PG 5MM+ 810.528 2.702 PI(34:2) PI 5MM+ 852.560 2.658 PI(20:4_16:0) PI 5MM+ 876.560 2.643 PI(20:4_18:0) PI 5MM+ 904.591 2.788 PI(20:4_18:1) PI 5MM+ 902.575 2.479 PI(18:1_18:0) PI 5MM+ 887.562 2.786 PI(18:2_18:0) PI 5MM+ 885.546 2.474 PI(O-43:9) PI 5MM+ 955.567 2.649 PS(18:0_18:2) PS 5MM+ 788.544 2.346 PS(18:0_20:3) PS 5MM+ 814.559 2.858 PS(18:0_20:4) PS 5MM+ 812.544 2.796 PS(18:0_22:5) PS 5MM+ 838.559 2.834 PS(18:1_20:4) PS 5MM+ 810.528 2.700 PS(20:4_20:4) PS 5MM+ 832.512 2.580 PS(20:4_22:6) PS 5MM+ 856.512 2.566 PS(20:4_18:0) PS 5MM+ 812.544 2.886 PS(38:4) PS 5MM+ 812.544 3.125 PS(34:1) PS 5MM+ 762.528 2.765 PS(20:4_16:0) PS 5MM+ 784.512 2.665 PS(36:2) PS 5MM+ 788.544 2.803 PS(20:4_18:2) PS 5MM+ 808.512 2.603 PS(20:5_18:0) PS 5MM+ 810.528 2.334 PS(22:4_18:0) PS 5MM+ 840.575 2.888 PS(O-38:4) PS 5MM+ 798.564 2.594 PS(20:4_20:2) PS 5MM+ 836.544 2.769 PS(20:4_17:0) PS 5MM+ 798.528 2.734 PS(18:3_18:0) PS 5MM+ 786.528 2.730 PS(20:4_22:3) PS 5MM+ 862.559 2.772 PS(42:9) PS 5MM+ 858.528 2.643 PS(34:2) PS 5MM+ 760.512 2.666 PS(40:7) PS 5MM+ 834.528 2.803 PS(4:0_18:0) PS 5MM+ 596.356 1.771 PS(10:0_18:0) PS 5MM+ 680.450 1.883 PS(21:4_18:0) PS 5MM+ 826.559 2.806 SM(d31:1) SM 5MM+ 661.528 2.613 SM(d32:1) SM 5MM+ 675.544 2.697 SM(d34:1) SM 5MM+ 703.575 2.847 SM(d35:2) SM 5MM+ 715.575 2.814 SM(d36:1) SM 5MM+ 731.606 2.980 SM(d40:1) SM 5MM+ 787.669 3.208 SM(d42:1) SM 5MM+ 815.700 3.313 SM(d42:2) SM 5MM+ 813.684 3.215 SM(d43:2) SM 5MM+ 827.700 3.266 SM(d14:0_20:1) SM 5MM+ 703.575 3.637 SM(d42:3) SM 5MM+ 811.669 3.141 SM(d42:4) SM 5MM+ 809.653 3.073 SM(d38:1) SM 5MM+ 759.637 3.095 SM(d30:1) SM 5MM+ 647.512 2.527 SM(d33:1) SM 5MM+ 689.559 2.779 SM(d34:2) SM 5MM+ 701.559 2.733 SM(d36:3) SM 5MM+ 727.575 2.789 SM(d36:2) SM 5MM+ 729.591 2.888 SM(d36:0) SM 5MM+ 733.622 3.035 SM(d38:2) SM 5MM+ 757.622 3.015 SM(d39:1) SM 5MM+ 773.653 3.161 SM(d40:3) SM 5MM+ 783.637 3.022 SM(d40:2) SM 5MM+ 785.653 3.119 SM(d44:2) SM 5MM+ 841.716 3.305 SM(d44:1) SM 5MM+ 843.731 3.405 SM(d33:2) SM 5MM+ 687.544 2.657 SM(d32:0) SM 5MM+ 677.559 2.760 SM(d38:3) SM 5MM+ 755.606 2.910 SM(d41:1) SM 5MM+ 801.684 3.267 SM(d44:5) SM 5MM+ 835.669 3.111 SM(d44:3) SM 5MM+ 839.700 3.234 SM(d42:5) SM 5MM+ 807.637 3.148 SM(d44:4) SM 5MM+ 859.666 3.166 SM(d44:6) SM 5MM+ 833.653 3.142 SM(d34:4) SM 5MM+ 697.528 2.723 SPH(d20:0) SPH 5MM+ 330.337 1.777 SPH(d16:1) SPH 5MM+ 272.258 1.659 SPH(d19:0) SPH 5MM+ 316.321 1.595 SPH(d17:0) SPH 5MM+ 288.290 1.174 SPH(d18:1) SPH 5MM+ 300.290 1.628 SPH(d16:0) SPH 5MM+ 274.274 0.924 TG(14:0_14:0_16:0) TG 5MM+ 768.708 3.623 TG(14:0_15:0_16:0) TG 5MM+ 782.723 3.642 TG(14:0_16:0_16:0) TG 5MM+ 796.739 3.665 TG(15:0_16:0_16:0) TG 5MM+ 810.755 3.685 TG(16:0_16:0_16:0) TG 5MM+ 824.770 3.706 TG(16:0_16:0_18:0) TG 5MM+ 852.801 3.740 TG(16:0_18:0_18:0) TG 5MM+ 880.833 3.781 TG(51:14_9:0) TG 5MM+ 964.739 3.654 TG(20:4_20:4_20:4) TG 5MM+ 968.770 3.742 TG(17:0_16:0_16:0) TG 5MM+ 838.786 3.720 TG(16:0_18:1_18:1) TG 5MM+ 876.801 3.706 TG(14:0_14:0_14:0) TG 5MM+ 740.676 3.569 TG(18:0_18:0_18:0) TG 5MM+ 908.864 3.803 TG(13:0_15:0_15:0) TG 5MM+ 754.692 3.591 TG(21:1) TG 5MM+ 444.332 1.360 TG(19:2) TG 5MM+ 414.285 0.758 TG(28:4_26:6_26:6) TG 5MM+ 1223.994 2.350 TG(18:1_18:1_18:1) TG 5MM+ 902.817 3.714 METABOLITES_END #END