#METABOLOMICS WORKBENCH iuk41_20240328_131847 DATATRACK_ID:4750 STUDY_ID:ST003150 ANALYSIS_ID:AN005168 PROJECT_ID:PR001958
VERSION             	1
CREATED_ON             	March 29, 2024, 11:56 am
#PROJECT
PR:PROJECT_TITLE                 	Impact of early-life exposure to a potent aryl hydrocarbon receptor ligand on
PR:PROJECT_TITLE                 	gut microbiota and host glucose homeostasis in C57BL/6J male mice
PR:PROJECT_SUMMARY               	This study aimed to explore the association between early-life exposure to a
PR:PROJECT_SUMMARY               	potent aryl hydrocarbon receptor (AHR) agonist and persistent disruptions in the
PR:PROJECT_SUMMARY               	microbiota, leading to impaired metabolic homeostasis later in life. This study
PR:PROJECT_SUMMARY               	utilized metagenomics, NMR- and mass spectrometry-based metabolomics, and
PR:PROJECT_SUMMARY               	biochemical assays to analyze the gut microbiome composition and function, as
PR:PROJECT_SUMMARY               	well as the physiological and metabolic effects of early-life exposure to
PR:PROJECT_SUMMARY               	2,3,7,8-tetrachlorodibenzofuran (TCDF) in conventional, germ-free (GF), and
PR:PROJECT_SUMMARY               	Ahr-null mice. The impact of TCDF on Akkermansia muciniphila (A. muciniphila) in
PR:PROJECT_SUMMARY               	vitro was assessed using optical density (OD 600), flow cytometry,
PR:PROJECT_SUMMARY               	transcriptomics, and mass spectrometry-based metabolomics. TCDF-exposed mice
PR:PROJECT_SUMMARY               	exhibited disruption in the gut microbiome community structure and function,
PR:PROJECT_SUMMARY               	characterized by lower abundances of A. muciniphila, lower levels of cecal short
PR:PROJECT_SUMMARY               	chain fatty acids (SCFAs) and indole-3-lactic acid (ILA), and a reduction in gut
PR:PROJECT_SUMMARY               	hormones GLP-1 and PYY. Importantly, microbial and metabolic phenotypes
PR:PROJECT_SUMMARY               	associated with early-life POP exposure were transferable to GF recipients in
PR:PROJECT_SUMMARY               	the absence of POP carry-over. In addition, AHR-independent interactions between
PR:PROJECT_SUMMARY               	POPs and the microbiota were observed, significantly affected the growth,
PR:PROJECT_SUMMARY               	physiology, gene expression, and metabolic activity of A. muciniphila, resulting
PR:PROJECT_SUMMARY               	in suppressed activity along the ILA pathway.
PR:INSTITUTE                     	Penn State University
PR:DEPARTMENT                    	Department of Veterinary and Biomedical Sciences
PR:LAST_NAME                     	Koo
PR:FIRST_NAME                    	Imhoi
PR:ADDRESS                       	307B Life Science Building
PR:EMAIL                         	iuk41@psu.edu
PR:PHONE                         	18148657803
#STUDY
ST:STUDY_TITLE                   	Impact of early-life exposure to a potent aryl hydrocarbon receptor ligand on
ST:STUDY_TITLE                   	gut microbiota and host glucose homeostasis in C57BL/6J male mice (Part II)
ST:STUDY_SUMMARY                 	This study aimed to explore the association between early-life exposure to a
ST:STUDY_SUMMARY                 	potent aryl hydrocarbon receptor (AHR) agonist and persistent disruptions in the
ST:STUDY_SUMMARY                 	microbiota, leading to impaired metabolic homeostasis later in life. This study
ST:STUDY_SUMMARY                 	utilized metagenomics, NMR- and mass spectrometry-based metabolomics, and
ST:STUDY_SUMMARY                 	biochemical assays to analyze the gut microbiome composition and function, as
ST:STUDY_SUMMARY                 	well as the physiological and metabolic effects of early-life exposure to
ST:STUDY_SUMMARY                 	2,3,7,8-tetrachlorodibenzofuran (TCDF) in conventional, germ-free (GF), and
ST:STUDY_SUMMARY                 	Ahr-null mice. The impact of TCDF on Akkermansia muciniphila (A. muciniphila) in
ST:STUDY_SUMMARY                 	vitro was assessed using optical density (OD 600), flow cytometry,
ST:STUDY_SUMMARY                 	transcriptomics, and mass spectrometry-based metabolomics. TCDF-exposed mice
ST:STUDY_SUMMARY                 	exhibited disruption in the gut microbiome community structure and function,
ST:STUDY_SUMMARY                 	characterized by lower abundances of A. muciniphila, lower levels of cecal short
ST:STUDY_SUMMARY                 	chain fatty acids (SCFAs) and indole-3-lactic acid (ILA), and a reduction in gut
ST:STUDY_SUMMARY                 	hormones GLP-1 and PYY. Importantly, microbial and metabolic phenotypes
ST:STUDY_SUMMARY                 	associated with early-life POP exposure were transferable to GF recipients in
ST:STUDY_SUMMARY                 	the absence of POP carry-over. In addition, AHR-independent interactions between
ST:STUDY_SUMMARY                 	POPs and the microbiota were observed, significantly affected the growth,
ST:STUDY_SUMMARY                 	physiology, gene expression, and metabolic activity of A. muciniphila, resulting
ST:STUDY_SUMMARY                 	in suppressed activity along the ILA pathway.
ST:INSTITUTE                     	Penn State University
ST:DEPARTMENT                    	Department of Veterinary and Biomedical Sciences
ST:LAST_NAME                     	Koo
ST:FIRST_NAME                    	Imhoi
ST:ADDRESS                       	307B Life Science Building
ST:EMAIL                         	iuk41@psu.edu
ST:PHONE                         	8148107425
#SUBJECT
SU:SUBJECT_TYPE                  	Mammal
SU:SUBJECT_SPECIES               	Mus musculus
SU:TAXONOMY_ID                   	10090
SU:AGE_OR_AGE_RANGE              	4 weeks to 5 months
SU:GENDER                        	Male
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data
SUBJECT_SAMPLE_FACTORS           	YT200819_02	YT200819_02	Sample source:liver | Treatment:vehicle	RAW_FILE_NAME=YT200819_02.CDF
SUBJECT_SAMPLE_FACTORS           	YT200819_03	YT200819_03	Sample source:liver | Treatment:vehicle	RAW_FILE_NAME=YT200819_03.CDF
SUBJECT_SAMPLE_FACTORS           	YT200819_04	YT200819_04	Sample source:liver | Treatment:vehicle	RAW_FILE_NAME=YT200819_04.CDF
SUBJECT_SAMPLE_FACTORS           	YT200819_05	YT200819_05	Sample source:liver | Treatment:vehicle	RAW_FILE_NAME=YT200819_05.CDF
SUBJECT_SAMPLE_FACTORS           	YT200819_06	YT200819_06	Sample source:liver | Treatment:vehicle	RAW_FILE_NAME=YT200819_06.CDF
SUBJECT_SAMPLE_FACTORS           	YT200819_07	YT200819_07	Sample source:liver | Treatment:vehicle	RAW_FILE_NAME=YT200819_07.CDF
SUBJECT_SAMPLE_FACTORS           	YT200819_08	YT200819_08	Sample source:liver | Treatment:TCDF	RAW_FILE_NAME=YT200819_08.CDF
SUBJECT_SAMPLE_FACTORS           	YT200819_09	YT200819_09	Sample source:liver | Treatment:TCDF	RAW_FILE_NAME=YT200819_09.CDF
SUBJECT_SAMPLE_FACTORS           	YT200819_10	YT200819_10	Sample source:liver | Treatment:TCDF	RAW_FILE_NAME=YT200819_10.CDF
SUBJECT_SAMPLE_FACTORS           	YT200819_11	YT200819_11	Sample source:liver | Treatment:TCDF	RAW_FILE_NAME=YT200819_11.CDF
SUBJECT_SAMPLE_FACTORS           	YT200819_12	YT200819_12	Sample source:liver | Treatment:TCDF	RAW_FILE_NAME=YT200819_12.CDF
SUBJECT_SAMPLE_FACTORS           	YT200819_13	YT200819_13	Sample source:liver | Treatment:TCDF	RAW_FILE_NAME=YT200819_13.CDF
SUBJECT_SAMPLE_FACTORS           	YT200819_21	YT200819_21	Sample source:liver | Treatment:vehicle	RAW_FILE_NAME=YT200819_21.CDF
SUBJECT_SAMPLE_FACTORS           	YT200819_22	YT200819_22	Sample source:liver | Treatment:vehicle	RAW_FILE_NAME=YT200819_22.CDF
SUBJECT_SAMPLE_FACTORS           	YT200819_101	YT200819_101	Sample source:liver | Treatment:vehicle	RAW_FILE_NAME=YT200819_101.CDF
SUBJECT_SAMPLE_FACTORS           	YT200819_102	YT200819_102	Sample source:liver | Treatment:vehicle	RAW_FILE_NAME=YT200819_102.CDF
SUBJECT_SAMPLE_FACTORS           	YT200819_103	YT200819_103	Sample source:liver | Treatment:vehicle	RAW_FILE_NAME=YT200819_103.CDF
SUBJECT_SAMPLE_FACTORS           	YT200819_104	YT200819_104	Sample source:liver | Treatment:vehicle	RAW_FILE_NAME=YT200819_104.CDF
SUBJECT_SAMPLE_FACTORS           	YT200819_105	YT200819_105	Sample source:liver | Treatment:TCDF	RAW_FILE_NAME=YT200819_105.CDF
SUBJECT_SAMPLE_FACTORS           	YT200819_106	YT200819_106	Sample source:liver | Treatment:TCDF	RAW_FILE_NAME=YT200819_106.CDF
SUBJECT_SAMPLE_FACTORS           	YT200819_107	YT200819_107	Sample source:liver | Treatment:TCDF	RAW_FILE_NAME=YT200819_107.CDF
SUBJECT_SAMPLE_FACTORS           	YT200819_108	YT200819_108	Sample source:liver | Treatment:TCDF	RAW_FILE_NAME=YT200819_108.CDF
SUBJECT_SAMPLE_FACTORS           	YT200819_109	YT200819_109	Sample source:liver | Treatment:TCDF	RAW_FILE_NAME=YT200819_109.CDF
SUBJECT_SAMPLE_FACTORS           	YT200819_110	YT200819_110	Sample source:liver | Treatment:TCDF	RAW_FILE_NAME=YT200819_110.CDF
SUBJECT_SAMPLE_FACTORS           	YT201026_14	YT201026_14	Sample source:liver | Treatment:TCDF	RAW_FILE_NAME=YT201026_14.CDF
SUBJECT_SAMPLE_FACTORS           	YT201026_15	YT201026_15	Sample source:liver | Treatment:TCDF	RAW_FILE_NAME=YT201026_15.CDF
SUBJECT_SAMPLE_FACTORS           	YT201026_16	YT201026_16	Sample source:liver | Treatment:TCDF	RAW_FILE_NAME=YT201026_16.CDF
SUBJECT_SAMPLE_FACTORS           	YT201026_17	YT201026_17	Sample source:liver | Treatment:TCDF	RAW_FILE_NAME=YT201026_17.CDF
SUBJECT_SAMPLE_FACTORS           	YT201026_18	YT201026_18	Sample source:liver | Treatment:TCDF	RAW_FILE_NAME=YT201026_18.CDF
SUBJECT_SAMPLE_FACTORS           	YT201026_19	YT201026_19	Sample source:liver | Treatment:TCDF	RAW_FILE_NAME=YT201026_19.CDF
SUBJECT_SAMPLE_FACTORS           	YT210427_16	YT210427_16	Sample source:liver | Treatment:TCDF	RAW_FILE_NAME=YT210427_16.CDF
SUBJECT_SAMPLE_FACTORS           	YT210427_17	YT210427_17	Sample source:liver | Treatment:TCDF	RAW_FILE_NAME=YT210427_17.CDF
SUBJECT_SAMPLE_FACTORS           	YT210427_18	YT210427_18	Sample source:liver | Treatment:TCDF	RAW_FILE_NAME=YT210427_18.CDF
SUBJECT_SAMPLE_FACTORS           	YT210427_19	YT210427_19	Sample source:liver | Treatment:TCDF	RAW_FILE_NAME=YT210427_19.CDF
SUBJECT_SAMPLE_FACTORS           	YT210427_20	YT210427_20	Sample source:liver | Treatment:TCDF	RAW_FILE_NAME=YT210427_20.CDF
SUBJECT_SAMPLE_FACTORS           	YT210427_21	YT210427_21	Sample source:liver | Treatment:TCDF	RAW_FILE_NAME=YT210427_21.CDF
#COLLECTION
CO:COLLECTION_SUMMARY            	Liver tissue samples were collected immediately after sacrifice by carbon
CO:COLLECTION_SUMMARY            	dioxide asphyxiation and kept at -80°C.
CO:SAMPLE_TYPE                   	Liver
#TREATMENT
TR:TREATMENT_SUMMARY             	1. After feed training, mice were fed pills containing TCDF (dissolved in
TR:TREATMENT_SUMMARY             	acetone) or acetone alone as vehicle, and one pill uniformly contained 0.46 µg
TR:TREATMENT_SUMMARY             	TCDF (24 µg/kg as final dose). Mice were housed singly in an empty cage and
TR:TREATMENT_SUMMARY             	monitored to ensure the pill was consumed in the morning for 5 days. 2. TCDF (24
TR:TREATMENT_SUMMARY             	µg/kg) or corn oil as vehicle were administered to age-matched male GF and
TR:TREATMENT_SUMMARY             	Ahr-/- mice by oral gavage once daily for five days (n = 4 per group). 3.
TR:TREATMENT_SUMMARY             	Four-week-old male GF C57BL/6J mice were orally gavaged with 100 µL of cecal
TR:TREATMENT_SUMMARY             	suspension (100 mg in 1 mL sterile BHI CHV media) from vehicle or TCDF treated
TR:TREATMENT_SUMMARY             	mice in long-duration model. 4. A. muciniphila was administered to GF mice by
TR:TREATMENT_SUMMARY             	oral gavage at one dose 107 CFU/0.1 mL suspended in sterile BHI CHV media
TR:TREATMENT_SUMMARY             	containing an end concentration of glycerol (15% vol/vol).
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	For liver lipid quantification, 50 mg of liver tissue was homogenized in 1 mL
SP:SAMPLEPREP_SUMMARY            	pre-cooled chloroform/methanol mix [1:1 (v/v)], followed by adding 296 µL
SP:SAMPLEPREP_SUMMARY            	water. After centrifugation (10,000 g, 4°C) for 10 min, the lower phase was
SP:SAMPLEPREP_SUMMARY            	dried in a vacuum and reconstituted in 600 µL of deuterated chloroform
SP:SAMPLEPREP_SUMMARY            	containing 0.03% (v/v) tetramethylsilane (TMS).
SP:SAMPLEPREP_PROTOCOL_FILENAME  	PSU_Methyl_esterification_GCMS_032724.pdf
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_TYPE           	GC
CH:INSTRUMENT_NAME               	Agilent 7890A
CH:COLUMN_NAME                   	Restek Rtx-5Sil MS (30m x 0.25mm, 0.25um)
CH:SOLVENT_A                     	N/A
CH:SOLVENT_B                     	N/A
CH:FLOW_GRADIENT                 	N/A
CH:FLOW_RATE                     	0.5658 mL/min
CH:COLUMN_TEMPERATURE            	325
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
AN:ANALYSIS_PROTOCOL_FILE        	PSU_tissue_POP_measurement_GCMS_032724.pdf
#MS
MS:INSTRUMENT_NAME               	Agilent 5975C
MS:INSTRUMENT_TYPE               	Single quadrupole
MS:MS_TYPE                       	EI
MS:ION_MODE                      	POSITIVE
MS:MS_COMMENTS                   	For ionization, the EI source was used with the following settings: filament
MS:MS_COMMENTS                   	source temperature at 230 °C and electron ionization energy at 70 eV. Mass
MS:MS_COMMENTS                   	spectra were collected in a mass range of m/z 50 - 600 at a scan rate of 2
MS:MS_COMMENTS                   	spectra/s. Mass calibration was performed after every injection with internal
MS:MS_COMMENTS                   	reference masses m/z 68.9947, 263.9866 and 501.9706. Data acquisition was
MS:MS_COMMENTS                   	performed using the Agilent MassHunter Workstation GC-MS Data Acquisition v 10.0
MS:MS_COMMENTS                   	(Agilent Technologies, Waldbronn, Germany).
#MS_METABOLITE_DATA
MS_METABOLITE_DATA:UNITS	peak area
MS_METABOLITE_DATA_START
Samples	YT201026_14	YT201026_15	YT201026_16	YT201026_17	YT201026_18	YT201026_19	YT210427_16	YT210427_17	YT210427_18	YT210427_19	YT210427_20	YT210427_21	YT200819_02	YT200819_03	YT200819_04	YT200819_05	YT200819_06	YT200819_07	YT200819_08	YT200819_09	YT200819_10	YT200819_11	YT200819_12	YT200819_13	YT200819_21	YT200819_22	YT200819_101	YT200819_102	YT200819_103	YT200819_104	YT200819_105	YT200819_106	YT200819_107	YT200819_108	YT200819_109	YT200819_110
Factors	Sample source:liver | Treatment:TCDF	Sample source:liver | Treatment:TCDF	Sample source:liver | Treatment:TCDF	Sample source:liver | Treatment:TCDF	Sample source:liver | Treatment:TCDF	Sample source:liver | Treatment:TCDF	Sample source:liver | Treatment:TCDF	Sample source:liver | Treatment:TCDF	Sample source:liver | Treatment:TCDF	Sample source:liver | Treatment:TCDF	Sample source:liver | Treatment:TCDF	Sample source:liver | Treatment:TCDF	Sample source:liver | Treatment:vehicle	Sample source:liver | Treatment:vehicle	Sample source:liver | Treatment:vehicle	Sample source:liver | Treatment:vehicle	Sample source:liver | Treatment:vehicle	Sample source:liver | Treatment:vehicle	Sample source:liver | Treatment:TCDF	Sample source:liver | Treatment:TCDF	Sample source:liver | Treatment:TCDF	Sample source:liver | Treatment:TCDF	Sample source:liver | Treatment:TCDF	Sample source:liver | Treatment:TCDF	Sample source:liver | Treatment:vehicle	Sample source:liver | Treatment:vehicle	Sample source:liver | Treatment:vehicle	Sample source:liver | Treatment:vehicle	Sample source:liver | Treatment:vehicle	Sample source:liver | Treatment:vehicle	Sample source:liver | Treatment:TCDF	Sample source:liver | Treatment:TCDF	Sample source:liver | Treatment:TCDF	Sample source:liver | Treatment:TCDF	Sample source:liver | Treatment:TCDF	Sample source:liver | Treatment:TCDF
TCDF	0.231168	0.154591	0.081734	0.074493	0.139774	0.1	0	0	0	0	0	0	N/A	N/A	N/A	N/A	N/A	N/A	N/A	N/A	N/A	N/A	N/A	N/A	N/A	N/A	N/A	N/A	N/A	N/A	N/A	N/A	N/A	N/A	N/A	N/A
C16:0	N/A	N/A	N/A	N/A	N/A	N/A	N/A	N/A	N/A	N/A	N/A	N/A	9.714356	9.109897	10.4442	9.076806	6.502155	10.12332	14.29972	9.591216	9.406372	14.98615	12.9385	14.97875	6.621081	7.273706	8.110182	7.866466	7.46785875	8.355032	9.493909	8.237781	7.313237	7.503699	8.570585	7.962943
C18:0	N/A	N/A	N/A	N/A	N/A	N/A	N/A	N/A	N/A	N/A	N/A	N/A	6.017603	4.540166	5.985824	5.727115	4.642378	6.064295	8.217515	5.850448	5.52072	8.382533	7.859274	8.854275	4.522949	4.601932	4.725817	4.443347	4.57351125	4.813667	6.064409	5.742851	4.769916	4.56904	4.630344	4.843543
C20:0	N/A	N/A	N/A	N/A	N/A	N/A	N/A	N/A	N/A	N/A	N/A	N/A	0.254823	0.108724	0.221872	0.217146	0.116565	0.241748	0.385404	0.230283	0.280408	0.394579	0.293472	0.390559	0.1820141	0.161377	0.201303	0.149316	0.173502525	0.195993	0.192734	0.47069	0.270261	0.260266	0.308677	0.282657
C22:0	N/A	N/A	N/A	N/A	N/A	N/A	N/A	N/A	N/A	N/A	N/A	N/A	0.411161	0.140726	0.250938	0.324135	0.152745	0.279568	0.509807	0.360928	0.409804	0.435558	0.302404	0.446042	0.17899	0.185431	0.232172	0.196098	0.19817275	0.193019	0.246087	0.42677	0.360714	0.277184	0.289124	0.307164
C16:1	N/A	N/A	N/A	N/A	N/A	N/A	N/A	N/A	N/A	N/A	N/A	N/A	0.307017	0.396873	0.253792	0.103565	0.133489	0.273937	0.655933	0.393816	0.440213	0.562749	0.543906	0.63114	0.199829	0.177241	0.333917	0.299978	0.25274125	0.403835	0.372302	0.291641	0.288244	0.325352	0.536589	0.291749
C18:1n9c	N/A	N/A	N/A	N/A	N/A	N/A	N/A	N/A	N/A	N/A	N/A	N/A	4.765205	4.116987	4.942037	3.306199	2.853414	4.294367	10.8908	4.736905	6.205129	11.15688	8.282143	11.29047	2.863554	2.768324	3.488835	3.407728	3.13211025	3.179089	4.217233	3.333326	2.834725	3.990053	3.596399	3.036793
C18:1n9t	N/A	N/A	N/A	N/A	N/A	N/A	N/A	N/A	N/A	N/A	N/A	N/A	0.462033	0.325371	0.354513	0.232263	0.229115	0.44264	0.733317	0.351766	0.557657	1.083112	0.881167	1.137568	0.243778	0.210788	0.288425	0.307486	0.26261925	0.350553	0.415015	0.248542	0.276078	0.454936	0.386068	0.229469
C18:2n6c	N/A	N/A	N/A	N/A	N/A	N/A	N/A	N/A	N/A	N/A	N/A	N/A	6.237328	4.607274	6.777583	5.306724	3.729595	5.787339	11.10535	6.16135	6.693592	10.55787	9.587518	12.39909	3.781076	4.22695	4.457052	4.425214	4.222573	4.688378	6.18487	5.600456	4.110624	4.68588	4.268127	4.705901
C20:3n6	N/A	N/A	N/A	N/A	N/A	N/A	N/A	N/A	N/A	N/A	N/A	N/A	0.293287	0.180116	0.171638	0.182365	0.182672	0.291583	0.553868	0.212256	0.421617	0.745912	0.759214	0.912221	0.289723	0.215301	0.363198	0.284193	0.28810375	0.314659	0.43487	0.323408	0.30834	0.39283	0.377142	0.324264
C20:4n6	N/A	N/A	N/A	N/A	N/A	N/A	N/A	N/A	N/A	N/A	N/A	N/A	2.736463	1.503002	2.096839	2.007292	1.431748	2.752923	2.866362	1.941899	1.916765	3.323658	3.367212	3.854757	1.904886	2.017933	2.04906	1.897615	1.9673735	2.145538	2.687853	2.584469	2.023868	2.454301	2.20458	2.126906
C20:5n3	N/A	N/A	N/A	N/A	N/A	N/A	N/A	N/A	N/A	N/A	N/A	N/A	0.246837	0.14886	0.217684	0.119579	0.124146	0.149129	0.197877	0.142309	0.179085	0.230901	0.203923	0.223852	0.185128	0.146787	0.172943	0.216656	0.1803785	0.179263	0.262064	0.234519	0.252109	0.154614	0.141463	0.263216
C22:6n3	N/A	N/A	N/A	N/A	N/A	N/A	N/A	N/A	N/A	N/A	N/A	N/A	3.017069	1.7347	2.215918	2.284509	1.451863	2.322459	3.179898	2.553364	2.220038	3.888389	3.463768	4.146459	1.98433	2.047307	2.334741	2.113849	2.12005675	2.280213	3.039368	2.872819	2.177372	2.278205	2.039791	2.497095
MS_METABOLITE_DATA_END
#METABOLITES
METABOLITES_START
metabolite_name	retention index	PubChem ID
TCDF	N/A	39929
C16:0	N/A	985
C18:0	N/A	5281
C20:0	N/A	10467
C22:0	N/A	8215
C16:1	N/A
C18:1n9c	N/A
C18:1n9t	N/A
C18:2n6c	N/A
C20:3n6	N/A
C20:4n6	N/A
C20:5n3	N/A
C22:6n3	N/A
METABOLITES_END
#END