#METABOLOMICS WORKBENCH MGJaramillo_20240605_124148 DATATRACK_ID:4904 STUDY_ID:ST003294 ANALYSIS_ID:AN005398 PROJECT_ID:PR002046
VERSION             	1
CREATED_ON             	July 5, 2024, 9:52 am
#PROJECT
PR:PROJECT_TITLE                 	Exploring the Effects of Toxic Road Runoff Contaminants (6PPD-quinone,
PR:PROJECT_TITLE                 	9,10-anthraquinone) on Juvenile Salmonids with a Multi-OMICs Approach
PR:PROJECT_TYPE                  	Untargeted Metabolomics
PR:PROJECT_SUMMARY               	Pacific salmonid populations have been declining predominantly due to
PR:PROJECT_SUMMARY               	anthropogenic factors such as climate change, habitat loss, and water
PR:PROJECT_SUMMARY               	contaminants derived from urban storm water runoff. Growing urbanism has
PR:PROJECT_SUMMARY               	resulted in areas of impervious surfaces leading to increased storm water runoff
PR:PROJECT_SUMMARY               	entering urban rivers and streams. Storm water runoff has been correlated to
PR:PROJECT_SUMMARY               	escalating rates of pre-spawn mortality (PSM) events in coho salmon in Pacific
PR:PROJECT_SUMMARY               	Northwest streams. PSM events in salmonids are characterized by rapid onset
PR:PROJECT_SUMMARY               	symptoms including surface swimming, loss of equilibrium, and fin splaying that
PR:PROJECT_SUMMARY               	precede mass adult population mortality prior to egg fertilization. In 2020, a
PR:PROJECT_SUMMARY               	chemical derived from tires,
PR:PROJECT_SUMMARY               	N-(1,3-Dimethylbutyl)-N'-phenyl-p-phenylenediamine-quinone (6Q), was isolated
PR:PROJECT_SUMMARY               	from stormwater runoff and subsequent toxicity tests resulted in acute coho
PR:PROJECT_SUMMARY               	salmon mortality exhibiting a 24-hour dose at fifty percent population mortality
PR:PROJECT_SUMMARY               	(LC50) of 95 ng/L. 6Q is an oxidation product derived from N-
PR:PROJECT_SUMMARY               	(1,3-Dimethylbutyl)-N'-phenyl-p-phenylenediamine (6PPD), which is used by tire
PR:PROJECT_SUMMARY               	manufacturers as an antioxidant and antiozonant. The toxic mechanism of 6Q is
PR:PROJECT_SUMMARY               	not yet known, but it is suspected to induce an inhibitory effect on cytochrome
PR:PROJECT_SUMMARY               	P450 (CYP450) genes encoding for CYP450 enzymes, which are responsible for
PR:PROJECT_SUMMARY               	xenobiotic metabolism. We hypothesize 6Q exposure affects salmonids’ ability
PR:PROJECT_SUMMARY               	to synthesize essential structural and cell signaling molecules and inhibits
PR:PROJECT_SUMMARY               	their ability to metabolize other water contaminants such as polycyclic
PR:PROJECT_SUMMARY               	hydrocarbons (PAHs). PAHs are a chemical class of concern due to their potential
PR:PROJECT_SUMMARY               	carcinogenic, teratogenic, and mutagenic effects in aquatic organisms. Thus,
PR:PROJECT_SUMMARY               	this project will focus on the commonly detected water PAH contaminant
PR:PROJECT_SUMMARY               	anthraquinone and its impact on salmonids when co-exposed to 6Q. Further,
PR:PROJECT_SUMMARY               	climate change may be a contributing factor to salmonid susceptibility to water
PR:PROJECT_SUMMARY               	contaminants. Increasing global carbon dioxide emissions have affected aquatic
PR:PROJECT_SUMMARY               	species significantly due to the ability of rivers and streams to transport or
PR:PROJECT_SUMMARY               	store atmospheric carbon dioxide, resulting in rapidly rising water temperatures
PR:PROJECT_SUMMARY               	having potential to exacerbate salmonid sensitivity to water contaminant
PR:INSTITUTE                     	Oregon State University
PR:DEPARTMENT                    	Environmental and Molecular Toxicology
PR:LABORATORY                    	Water Quality Toxicology
PR:LAST_NAME                     	Garcia-Jaramillo
PR:FIRST_NAME                    	Manuel
PR:ADDRESS                       	1007 SW Campus Way, Corvallis, OR, 97331
PR:EMAIL                         	manuel.g.jaramillo@oregonstate.edu
PR:PHONE                         	541-737-5714
#STUDY
ST:STUDY_TITLE                   	Exploring the Effects of Toxic Road Runoff Contaminants (6PPD-quinone,
ST:STUDY_TITLE                   	9,10-anthraquinone) on Juvenile Salmonids with a Multi-OMICs Approach (Brain)
ST:STUDY_SUMMARY                 	Pacific salmonid populations have been declining predominantly due to
ST:STUDY_SUMMARY                 	anthropogenic factors such as climate change, habitat loss, and water
ST:STUDY_SUMMARY                 	contaminants derived from urban storm water runoff. Growing urbanism has
ST:STUDY_SUMMARY                 	resulted in areas of impervious surfaces leading to increased storm water runoff
ST:STUDY_SUMMARY                 	entering urban rivers and streams. Storm water runoff has been correlated to
ST:STUDY_SUMMARY                 	escalating rates of pre-spawn mortality (PSM) events in coho salmon in Pacific
ST:STUDY_SUMMARY                 	Northwest streams. PSM events in salmonids are characterized by rapid onset
ST:STUDY_SUMMARY                 	symptoms including surface swimming, loss of equilibrium, and fin splaying that
ST:STUDY_SUMMARY                 	precede mass adult population mortality prior to egg fertilization. In 2020, a
ST:STUDY_SUMMARY                 	chemical derived from tires,
ST:STUDY_SUMMARY                 	N-(1,3-Dimethylbutyl)-N'-phenyl-p-phenylenediamine-quinone (6Q), was isolated
ST:STUDY_SUMMARY                 	from storm water runoff and subsequent toxicity tests resulted in acute coho
ST:STUDY_SUMMARY                 	salmon mortality exhibiting a 24-hour dose at fifty percent population mortality
ST:STUDY_SUMMARY                 	(LC50) of 95 ng/L. 6Q is an oxidation product derived from
ST:STUDY_SUMMARY                 	N-(1,3-Dimethylbutyl)-N'-phenyl-p-phenylenediamine (6PPD), which is used by tire
ST:STUDY_SUMMARY                 	manufacturers as an antioxidant and antiozonant. The toxic mechanism of 6Q is
ST:STUDY_SUMMARY                 	not yet known, but it is suspected to induce an inhibitory effect on cytochrome
ST:STUDY_SUMMARY                 	P450 (CYP450) genes encoding for CYP450 enzymes, which are responsible for
ST:STUDY_SUMMARY                 	xenobiotic metabolism. We hypothesize 6Q exposure affects salmonids’ ability
ST:STUDY_SUMMARY                 	to synthesize essential structural and cell signaling molecules and inhibits
ST:STUDY_SUMMARY                 	their ability to metabolize other water contaminants such as polycyclic
ST:STUDY_SUMMARY                 	hydrocarbons (PAHs). PAHs are a chemical class of concern due to their potential
ST:STUDY_SUMMARY                 	carcinogenic, teratogenic, and mutagenic effects in aquatic organisms. Thus,
ST:STUDY_SUMMARY                 	this project will focus on the commonly detected water PAH contaminant
ST:STUDY_SUMMARY                 	anthraquinone and its impact on salmonids when co-exposed to 6Q. Further,
ST:STUDY_SUMMARY                 	climate change may be a contributing factor to salmonid susceptibility to water
ST:STUDY_SUMMARY                 	contaminants. Increasing global carbon dioxide emissions have affected aquatic
ST:STUDY_SUMMARY                 	species significantly due to the ability of rivers and streams to transport or
ST:STUDY_SUMMARY                 	store atmospheric carbon dioxide, resulting in rapidly rising water temperatures
ST:STUDY_SUMMARY                 	having potential to exacerbate salmonid sensitivity to water contaminant
ST:INSTITUTE                     	Oregon State University
ST:DEPARTMENT                    	Environmental and Molecular Toxicology
ST:LABORATORY                    	Water Quality Toxicology
ST:LAST_NAME                     	Garcia-Jaramillo
ST:FIRST_NAME                    	Manuel
ST:ADDRESS                       	1007 SW Campus Way, Corvallis, OR, 97331
ST:EMAIL                         	manuel.g.jaramillo@oregonstate.edu
ST:NUM_GROUPS                    	6
ST:TOTAL_SUBJECTS                	270
ST:NUM_MALES                     	NA
ST:NUM_FEMALES                   	NA
ST:STUDY_TYPE                    	Metabolomics
ST:PHONE                         	541-737-5714
#SUBJECT
SU:SUBJECT_TYPE                  	Fish
SU:SUBJECT_SPECIES               	Oncorhynchus tshawytscha, Oncorhynchus mykiss, Oncorhynchus kisutch
SU:TAXONOMY_ID                   	74940, 8022, 8019
SU:WEIGHT_OR_WEIGHT_RANGE        	3-5 g
SU:HEIGHT_OR_HEIGHT_RANGE        	NA
#FACTORS
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data
SUBJECT_SAMPLE_FACTORS           	46	46_UC_B5_3_RT	Treatment:Unfed Control | Sample source:Brain | Species:Rainbow Trout	Tank Replicate=B5; Experimental Replicate=3; RAW_FILE_NAME=46_UC_B5_3_RT.mzML
SUBJECT_SAMPLE_FACTORS           	99	99_UC_B5_3_CO	Treatment:Unfed Control | Sample source:Brain | Species:Coho	Tank Replicate=B5; Experimental Replicate=3; RAW_FILE_NAME=99_UC_B5_3_CO.mzML
SUBJECT_SAMPLE_FACTORS           	12	12_UC_B5_3_CH	Treatment:Unfed Control | Sample source:Brain | Species:Chinook	Tank Replicate=B5; Experimental Replicate=3; RAW_FILE_NAME=12_UC_B5_3_CH.mzML
SUBJECT_SAMPLE_FACTORS           	170	170_UC_B5_2_RT	Treatment:Unfed Control | Sample source:Brain | Species:Rainbow Trout	Tank Replicate=B5; Experimental Replicate=2; RAW_FILE_NAME=170_UC_B5_2_RT.mzML
SUBJECT_SAMPLE_FACTORS           	64	64_UC_B5_2_CO	Treatment:Unfed Control | Sample source:Brain | Species:Coho	Tank Replicate=B5; Experimental Replicate=2; RAW_FILE_NAME=64_UC_B5_2_CO.mzML
SUBJECT_SAMPLE_FACTORS           	191	191_UC_B5_2_CH	Treatment:Unfed Control | Sample source:Brain | Species:Chinook	Tank Replicate=B5; Experimental Replicate=2; RAW_FILE_NAME=191_UC_B5_2_CH.mzML
SUBJECT_SAMPLE_FACTORS           	187	187_UC_B5_1_RT	Treatment:Unfed Control | Sample source:Brain | Species:Rainbow Trout	Tank Replicate=B5; Experimental Replicate=1; RAW_FILE_NAME=187_UC_B5_1_RT.mzML
SUBJECT_SAMPLE_FACTORS           	82	82_UC_B5_1_CO	Treatment:Unfed Control | Sample source:Brain | Species:Coho	Tank Replicate=B5; Experimental Replicate=1; RAW_FILE_NAME=82_UC_B5_1_CO.mzML
SUBJECT_SAMPLE_FACTORS           	201	201_UC_B5_1_CH	Treatment:Unfed Control | Sample source:Brain | Species:Chinook	Tank Replicate=B5; Experimental Replicate=1; RAW_FILE_NAME=201_UC_B5_1_CH.mzML
SUBJECT_SAMPLE_FACTORS           	95	95_IN_B5_3_RT	Treatment:Insult | Sample source:Brain | Species:Rainbow Trout	Tank Replicate=B5; Experimental Replicate=3; RAW_FILE_NAME=95_IN_B5_3_RT.mzML
SUBJECT_SAMPLE_FACTORS           	263	263_IN_B5_3_CO	Treatment:Insult | Sample source:Brain | Species:Coho	Tank Replicate=B5; Experimental Replicate=3; RAW_FILE_NAME=263_IN_B5_3_CO.mzML
SUBJECT_SAMPLE_FACTORS           	36	36_IN_B5_3_CH	Treatment:Insult | Sample source:Brain | Species:Chinook	Tank Replicate=B5; Experimental Replicate=3; RAW_FILE_NAME=36_IN_B5_3_CH.mzML
SUBJECT_SAMPLE_FACTORS           	118	118_IN_B5_2_RT	Treatment:Insult | Sample source:Brain | Species:Rainbow Trout	Tank Replicate=B5; Experimental Replicate=2; RAW_FILE_NAME=118_IN_B5_2_RT.mzML
SUBJECT_SAMPLE_FACTORS           	177	177_IN_B5_2_CO	Treatment:Insult | Sample source:Brain | Species:Coho	Tank Replicate=B5; Experimental Replicate=2; RAW_FILE_NAME=177_IN_B5_2_CO.mzML
SUBJECT_SAMPLE_FACTORS           	34	34_IN_B5_2_CH	Treatment:Insult | Sample source:Brain | Species:Chinook	Tank Replicate=B5; Experimental Replicate=2; RAW_FILE_NAME=34_IN_B5_2_CH.mzML
SUBJECT_SAMPLE_FACTORS           	66	66_IN_B5_1_RT	Treatment:Insult | Sample source:Brain | Species:Rainbow Trout	Tank Replicate=B5; Experimental Replicate=1; RAW_FILE_NAME=66_IN_B5_1_RT.mzML
SUBJECT_SAMPLE_FACTORS           	186	186_IN_B5_1_CH	Treatment:Insult | Sample source:Brain | Species:Chinook	Tank Replicate=B5; Experimental Replicate=1; RAW_FILE_NAME=186_IN_B5_1_CH.mzML
SUBJECT_SAMPLE_FACTORS           	163	163_FC_B5_3_RT	Treatment:Fed Control | Sample source:Brain | Species:Rainbow Trout	Tank Replicate=B5; Experimental Replicate=3; RAW_FILE_NAME=163_FC_B5_3_RT.mzML
SUBJECT_SAMPLE_FACTORS           	77	77_FC_B5_3_CO	Treatment:Fed Control | Sample source:Brain | Species:Coho	Tank Replicate=B5; Experimental Replicate=3; RAW_FILE_NAME=77_FC_B5_3_CO.mzML
SUBJECT_SAMPLE_FACTORS           	264	264_FC_B5_3_CH	Treatment:Fed Control | Sample source:Brain | Species:Chinook	Tank Replicate=B5; Experimental Replicate=3; RAW_FILE_NAME=264_FC_B5_3_CH.mzML
SUBJECT_SAMPLE_FACTORS           	60	60_FC_B5_2_RT	Treatment:Fed Control | Sample source:Brain | Species:Rainbow Trout	Tank Replicate=B5; Experimental Replicate=2; RAW_FILE_NAME=60_FC_B5_2_RT.mzML
SUBJECT_SAMPLE_FACTORS           	181	181_FC_B5_2_CO	Treatment:Fed Control | Sample source:Brain | Species:Coho	Tank Replicate=B5; Experimental Replicate=2; RAW_FILE_NAME=181_FC_B5_2_CO.mzML
SUBJECT_SAMPLE_FACTORS           	17	17_FC_B5_2_CH	Treatment:Fed Control | Sample source:Brain | Species:Chinook	Tank Replicate=B5; Experimental Replicate=2; RAW_FILE_NAME=17_FC_B5_2_CH.mzML
SUBJECT_SAMPLE_FACTORS           	128	128_FC_B5_1_RT	Treatment:Fed Control | Sample source:Brain | Species:Rainbow Trout	Tank Replicate=B5; Experimental Replicate=1; RAW_FILE_NAME=128_FC_B5_1_RT.mzML
SUBJECT_SAMPLE_FACTORS           	246	246_FC_B5_1_CH	Treatment:Fed Control | Sample source:Brain | Species:Chinook	Tank Replicate=B5; Experimental Replicate=1; RAW_FILE_NAME=246_FC_B5_1_CH.mzML
SUBJECT_SAMPLE_FACTORS           	183	183_CE_B5_3_RT	Treatment:Coexposure | Sample source:Brain | Species:Rainbow Trout	Tank Replicate=B5; Experimental Replicate=3; RAW_FILE_NAME=183_CE_B5_3_RT.mzML
SUBJECT_SAMPLE_FACTORS           	205	205_CE_B5_3_CO	Treatment:Coexposure | Sample source:Brain | Species:Coho	Tank Replicate=B5; Experimental Replicate=3; RAW_FILE_NAME=205_CE_B5_3_CO.mzML
SUBJECT_SAMPLE_FACTORS           	49	49_CE_B5_3_CH	Treatment:Coexposure | Sample source:Brain | Species:Chinook	Tank Replicate=B5; Experimental Replicate=3; RAW_FILE_NAME=49_CE_B5_3_CH.mzML
SUBJECT_SAMPLE_FACTORS           	184	184_CE_B5_2_RT	Treatment:Coexposure | Sample source:Brain | Species:Rainbow Trout	Tank Replicate=B5; Experimental Replicate=2; RAW_FILE_NAME=184_CE_B5_2_RT.mzML
SUBJECT_SAMPLE_FACTORS           	68	68_CE_B5_2_CO	Treatment:Coexposure | Sample source:Brain | Species:Coho	Tank Replicate=B5; Experimental Replicate=2; RAW_FILE_NAME=68_CE_B5_2_CO.mzML
SUBJECT_SAMPLE_FACTORS           	14	14_CE_B5_1_RT	Treatment:Coexposure | Sample source:Brain | Species:Rainbow Trout	Tank Replicate=B5; Experimental Replicate=1; RAW_FILE_NAME=14_CE_B5_1_RT.mzML
SUBJECT_SAMPLE_FACTORS           	165	165_CE_B5_1_CO	Treatment:Coexposure | Sample source:Brain | Species:Coho	Tank Replicate=B5; Experimental Replicate=1; RAW_FILE_NAME=165_CE_B5_1_CO.mzML
SUBJECT_SAMPLE_FACTORS           	18	18_CE_B5_1_CH	Treatment:Coexposure | Sample source:Brain | Species:Chinook	Tank Replicate=B5; Experimental Replicate=1; RAW_FILE_NAME=18_CE_B5_1_CH.mzML
SUBJECT_SAMPLE_FACTORS           	227	227_AQ_B5_3_CO	Treatment:Anthraquinone | Sample source:Brain | Species:Coho	Tank Replicate=B5; Experimental Replicate=3; RAW_FILE_NAME=227_AQ_B5_3_CO.mzML
SUBJECT_SAMPLE_FACTORS           	72	72_AQ_B5_3_CH	Treatment:Anthraquinone | Sample source:Brain | Species:Chinook	Tank Replicate=B5; Experimental Replicate=3; RAW_FILE_NAME=72_AQ_B5_3_CH.mzML
SUBJECT_SAMPLE_FACTORS           	86	86_AQ_B5_2_RT	Treatment:Anthraquinone | Sample source:Brain | Species:Rainbow Trout	Tank Replicate=B5; Experimental Replicate=2; RAW_FILE_NAME=86_AQ_B5_2_RT.mzML
SUBJECT_SAMPLE_FACTORS           	226	226_AQ_B5_2_CO	Treatment:Anthraquinone | Sample source:Brain | Species:Coho	Tank Replicate=B5; Experimental Replicate=2; RAW_FILE_NAME=226_AQ_B5_2_CO.mzML
SUBJECT_SAMPLE_FACTORS           	1	1_AQ_B5_2_CH	Treatment:Anthraquinone | Sample source:Brain | Species:Chinook	Tank Replicate=B5; Experimental Replicate=2; RAW_FILE_NAME=1_AQ_B5_2_CH.mzML
SUBJECT_SAMPLE_FACTORS           	243	243_AQ_B5_1_RT	Treatment:Anthraquinone | Sample source:Brain | Species:Rainbow Trout	Tank Replicate=B5; Experimental Replicate=1; RAW_FILE_NAME=243_AQ_B5_1_RT.mzML
SUBJECT_SAMPLE_FACTORS           	195	195_AQ_B5_1_CO	Treatment:Anthraquinone | Sample source:Brain | Species:Coho	Tank Replicate=B5; Experimental Replicate=1; RAW_FILE_NAME=195_AQ_B5_1_CO.mzML
SUBJECT_SAMPLE_FACTORS           	149	149_AQ_B5_1_CH	Treatment:Anthraquinone | Sample source:Brain | Species:Chinook	Tank Replicate=B5; Experimental Replicate=1; RAW_FILE_NAME=149_AQ_B5_1_CH.mzML
SUBJECT_SAMPLE_FACTORS           	121	121_6Q_B5_3_RT	Treatment:6PPD-quinone | Sample source:Brain | Species:Rainbow Trout	Tank Replicate=B5; Experimental Replicate=3; RAW_FILE_NAME=121_6Q_B5_3_RT.mzML
SUBJECT_SAMPLE_FACTORS           	162	162_6Q_B5_3_CO	Treatment:6PPD-quinone | Sample source:Brain | Species:Coho	Tank Replicate=B5; Experimental Replicate=3; RAW_FILE_NAME=162_6Q_B5_3_CO.mzML
SUBJECT_SAMPLE_FACTORS           	76	76_6Q_B5_3_CH	Treatment:6PPD-quinone | Sample source:Brain | Species:Chinook	Tank Replicate=B5; Experimental Replicate=3; RAW_FILE_NAME=76_6Q_B5_3_CH.mzML
SUBJECT_SAMPLE_FACTORS           	257	257_6Q_B5_2_RT	Treatment:6PPD-quinone | Sample source:Brain | Species:Rainbow Trout	Tank Replicate=B5; Experimental Replicate=2; RAW_FILE_NAME=257_6Q_B5_2_RT.mzML
SUBJECT_SAMPLE_FACTORS           	193	193_6Q_B5_2_CO	Treatment:6PPD-quinone | Sample source:Brain | Species:Coho	Tank Replicate=B5; Experimental Replicate=2; RAW_FILE_NAME=193_6Q_B5_2_CO.mzML
SUBJECT_SAMPLE_FACTORS           	223	223_6Q_B5_2_CH	Treatment:6PPD-quinone | Sample source:Brain | Species:Chinook	Tank Replicate=B5; Experimental Replicate=2; RAW_FILE_NAME=223_6Q_B5_2_CH.mzML
SUBJECT_SAMPLE_FACTORS           	270	270_6Q_B5_1_RT	Treatment:6PPD-quinone | Sample source:Brain | Species:Rainbow Trout	Tank Replicate=B5; Experimental Replicate=1; RAW_FILE_NAME=270_6Q_B5_1_RT.mzML
SUBJECT_SAMPLE_FACTORS           	129	129_6Q_B5_1_CO	Treatment:6PPD-quinone | Sample source:Brain | Species:Coho	Tank Replicate=B5; Experimental Replicate=1; RAW_FILE_NAME=129_6Q_B5_1_CO.mzML
SUBJECT_SAMPLE_FACTORS           	124	124_6Q_B5_1_CH	Treatment:6PPD-quinone | Sample source:Brain | Species:Chinook	Tank Replicate=B5; Experimental Replicate=1; RAW_FILE_NAME=124_6Q_B5_1_CH.mzML
SUBJECT_SAMPLE_FACTORS           	40	40_UC_B4_3_RT	Treatment:Unfed Control | Sample source:Brain | Species:Rainbow Trout	Tank Replicate=B4; Experimental Replicate=3; RAW_FILE_NAME=40_UC_B4_3_RT.mzML
SUBJECT_SAMPLE_FACTORS           	24	24_UC_B4_3_CO	Treatment:Unfed Control | Sample source:Brain | Species:Coho	Tank Replicate=B4; Experimental Replicate=3; RAW_FILE_NAME=24_UC_B4_3_CO.mzML
SUBJECT_SAMPLE_FACTORS           	152	152_UC_B4_3_CH	Treatment:Unfed Control | Sample source:Brain | Species:Chinook	Tank Replicate=B4; Experimental Replicate=3; RAW_FILE_NAME=152_UC_B4_3_CH.mzML
SUBJECT_SAMPLE_FACTORS           	59	59_UC_B4_2_RT	Treatment:Unfed Control | Sample source:Brain | Species:Rainbow Trout	Tank Replicate=B4; Experimental Replicate=2; RAW_FILE_NAME=59_UC_B4_2_RT.mzML
SUBJECT_SAMPLE_FACTORS           	13	13_UC_B4_2_CO	Treatment:Unfed Control | Sample source:Brain | Species:Coho	Tank Replicate=B4; Experimental Replicate=2; RAW_FILE_NAME=13_UC_B4_2_CO.mzML
SUBJECT_SAMPLE_FACTORS           	150	150_UC_B4_2_CH	Treatment:Unfed Control | Sample source:Brain | Species:Chinook	Tank Replicate=B4; Experimental Replicate=2; RAW_FILE_NAME=150_UC_B4_2_CH.mzML
SUBJECT_SAMPLE_FACTORS           	58	58_UC_B4_1_RT	Treatment:Unfed Control | Sample source:Brain | Species:Rainbow Trout	Tank Replicate=B4; Experimental Replicate=1; RAW_FILE_NAME=58_UC_B4_1_RT.mzML
SUBJECT_SAMPLE_FACTORS           	104	104_UC_B4_1_CO	Treatment:Unfed Control | Sample source:Brain | Species:Coho	Tank Replicate=B4; Experimental Replicate=1; RAW_FILE_NAME=104_UC_B4_1_CO.mzML
SUBJECT_SAMPLE_FACTORS           	157	157_UC_B4_1_CH	Treatment:Unfed Control | Sample source:Brain | Species:Chinook	Tank Replicate=B4; Experimental Replicate=1; RAW_FILE_NAME=157_UC_B4_1_CH.mzML
SUBJECT_SAMPLE_FACTORS           	260	260_IN_B4_3_RT	Treatment:Insult | Sample source:Brain | Species:Rainbow Trout	Tank Replicate=B4; Experimental Replicate=3; RAW_FILE_NAME=260_IN_B4_3_RT.mzML
SUBJECT_SAMPLE_FACTORS           	94	94_IN_B4_3_CO	Treatment:Insult | Sample source:Brain | Species:Coho	Tank Replicate=B4; Experimental Replicate=3; RAW_FILE_NAME=94_IN_B4_3_CO.mzML
SUBJECT_SAMPLE_FACTORS           	78	78_IN_B4_3_CH	Treatment:Insult | Sample source:Brain | Species:Chinook	Tank Replicate=B4; Experimental Replicate=3; RAW_FILE_NAME=78_IN_B4_3_CH.mzML
SUBJECT_SAMPLE_FACTORS           	231	231_IN_B4_2_RT	Treatment:Insult | Sample source:Brain | Species:Rainbow Trout	Tank Replicate=B4; Experimental Replicate=2; RAW_FILE_NAME=231_IN_B4_2_RT.mzML
SUBJECT_SAMPLE_FACTORS           	220	220_IN_B4_2_CO	Treatment:Insult | Sample source:Brain | Species:Coho	Tank Replicate=B4; Experimental Replicate=2; RAW_FILE_NAME=220_IN_B4_2_CO.mzML
SUBJECT_SAMPLE_FACTORS           	154	154_IN_B4_2_CH	Treatment:Insult | Sample source:Brain | Species:Chinook	Tank Replicate=B4; Experimental Replicate=2; RAW_FILE_NAME=154_IN_B4_2_CH.mzML
SUBJECT_SAMPLE_FACTORS           	120	120_IN_B4_1_RT	Treatment:Insult | Sample source:Brain | Species:Rainbow Trout	Tank Replicate=B4; Experimental Replicate=1; RAW_FILE_NAME=120_IN_B4_1_RT.mzML
SUBJECT_SAMPLE_FACTORS           	44	44_IN_B4_1_CO	Treatment:Insult | Sample source:Brain | Species:Coho	Tank Replicate=B4; Experimental Replicate=1; RAW_FILE_NAME=44_IN_B4_1_CO.mzML
SUBJECT_SAMPLE_FACTORS           	228	228_IN_B4_1_CH	Treatment:Insult | Sample source:Brain | Species:Chinook	Tank Replicate=B4; Experimental Replicate=1; RAW_FILE_NAME=228_IN_B4_1_CH.mzML
SUBJECT_SAMPLE_FACTORS           	100	100_FC_B4_3_RT	Treatment:Fed Control | Sample source:Brain | Species:Rainbow Trout	Tank Replicate=B4; Experimental Replicate=3; RAW_FILE_NAME=100_FC_B4_3_RT.mzML
SUBJECT_SAMPLE_FACTORS           	132	132_FC_B4_3_CO	Treatment:Fed Control | Sample source:Brain | Species:Coho	Tank Replicate=B4; Experimental Replicate=3; RAW_FILE_NAME=132_FC_B4_3_CO.mzML
SUBJECT_SAMPLE_FACTORS           	16	16_FC_B4_3_CH	Treatment:Fed Control | Sample source:Brain | Species:Chinook	Tank Replicate=B4; Experimental Replicate=3; RAW_FILE_NAME=16_FC_B4_3_CH.mzML
SUBJECT_SAMPLE_FACTORS           	178	178_FC_B4_2_RT	Treatment:Fed Control | Sample source:Brain | Species:Rainbow Trout	Tank Replicate=B4; Experimental Replicate=2; RAW_FILE_NAME=178_FC_B4_2_RT.mzML
SUBJECT_SAMPLE_FACTORS           	136	136_FC_B4_2_CO	Treatment:Fed Control | Sample source:Brain | Species:Coho	Tank Replicate=B4; Experimental Replicate=2; RAW_FILE_NAME=136_FC_B4_2_CO.mzML
SUBJECT_SAMPLE_FACTORS           	237	237_FC_B4_2_CH	Treatment:Fed Control | Sample source:Brain | Species:Chinook	Tank Replicate=B4; Experimental Replicate=2; RAW_FILE_NAME=237_FC_B4_2_CH.mzML
SUBJECT_SAMPLE_FACTORS           	35	35_FC_B4_1_RT	Treatment:Fed Control | Sample source:Brain | Species:Rainbow Trout	Tank Replicate=B4; Experimental Replicate=1; RAW_FILE_NAME=35_FC_B4_1_RT.mzML
SUBJECT_SAMPLE_FACTORS           	241	241_FC_B4_1_CO	Treatment:Fed Control | Sample source:Brain | Species:Coho	Tank Replicate=B4; Experimental Replicate=1; RAW_FILE_NAME=241_FC_B4_1_CO.mzML
SUBJECT_SAMPLE_FACTORS           	141	141_FC_B4_1_CH	Treatment:Fed Control | Sample source:Brain | Species:Chinook	Tank Replicate=B4; Experimental Replicate=1; RAW_FILE_NAME=141_FC_B4_1_CH.mzML
SUBJECT_SAMPLE_FACTORS           	233	233_CE_B4_3_RT	Treatment:Coexposure | Sample source:Brain | Species:Rainbow Trout	Tank Replicate=B4; Experimental Replicate=3; RAW_FILE_NAME=233_CE_B4_3_RT.mzML
SUBJECT_SAMPLE_FACTORS           	9	9_CE_B4_3_CO	Treatment:Coexposure | Sample source:Brain | Species:Coho	Tank Replicate=B4; Experimental Replicate=3; RAW_FILE_NAME=9_CE_B4_3_CO.mzML
SUBJECT_SAMPLE_FACTORS           	123	123_CE_B4_3_CH	Treatment:Coexposure | Sample source:Brain | Species:Chinook	Tank Replicate=B4; Experimental Replicate=3; RAW_FILE_NAME=123_CE_B4_3_CH.mzML
SUBJECT_SAMPLE_FACTORS           	172	172_CE_B4_2_CO	Treatment:Coexposure | Sample source:Brain | Species:Coho	Tank Replicate=B4; Experimental Replicate=2; RAW_FILE_NAME=172_CE_B4_2_CO.mzML
SUBJECT_SAMPLE_FACTORS           	106	106_CE_B4_2_CH	Treatment:Coexposure | Sample source:Brain | Species:Chinook	Tank Replicate=B4; Experimental Replicate=2; RAW_FILE_NAME=106_CE_B4_2_CH.mzML
SUBJECT_SAMPLE_FACTORS           	32	32_CE_B4_1_RT	Treatment:Coexposure | Sample source:Brain | Species:Rainbow Trout	Tank Replicate=B4; Experimental Replicate=1; RAW_FILE_NAME=32_CE_B4_1_RT.mzML
SUBJECT_SAMPLE_FACTORS           	6	6_CE_B4_1_CO	Treatment:Coexposure | Sample source:Brain | Species:Coho	Tank Replicate=B4; Experimental Replicate=1; RAW_FILE_NAME=6_CE_B4_1_CO.mzML
SUBJECT_SAMPLE_FACTORS           	53	53_CE_B4_1_CH	Treatment:Coexposure | Sample source:Brain | Species:Chinook	Tank Replicate=B4; Experimental Replicate=1; RAW_FILE_NAME=53_CE_B4_1_CH.mzML
SUBJECT_SAMPLE_FACTORS           	256	256_AQ_B4_3_RT	Treatment:Anthraquinone | Sample source:Brain | Species:Rainbow Trout	Tank Replicate=B4; Experimental Replicate=3; RAW_FILE_NAME=256_AQ_B4_3_RT.mzML
SUBJECT_SAMPLE_FACTORS           	110	110_AQ_B4_3_CO	Treatment:Anthraquinone | Sample source:Brain | Species:Coho	Tank Replicate=B4; Experimental Replicate=3; RAW_FILE_NAME=110_AQ_B4_3_CO.mzML
SUBJECT_SAMPLE_FACTORS           	174	174_AQ_B4_3_CH	Treatment:Anthraquinone | Sample source:Brain | Species:Chinook	Tank Replicate=B4; Experimental Replicate=3; RAW_FILE_NAME=174_AQ_B4_3_CH.mzML
SUBJECT_SAMPLE_FACTORS           	23	23_AQ_B4_2_CO	Treatment:Anthraquinone | Sample source:Brain | Species:Coho	Tank Replicate=B4; Experimental Replicate=2; RAW_FILE_NAME=23_AQ_B4_2_CO.mzML
SUBJECT_SAMPLE_FACTORS           	26	26_AQ_B4_2_CH	Treatment:Anthraquinone | Sample source:Brain | Species:Chinook	Tank Replicate=B4; Experimental Replicate=2; RAW_FILE_NAME=26_AQ_B4_2_CH.mzML
SUBJECT_SAMPLE_FACTORS           	22	22_AQ_B4_1_RT	Treatment:Anthraquinone | Sample source:Brain | Species:Rainbow Trout	Tank Replicate=B4; Experimental Replicate=1; RAW_FILE_NAME=22_AQ_B4_1_RT.mzML
SUBJECT_SAMPLE_FACTORS           	164	164_AQ_B4_1_CO	Treatment:Anthraquinone | Sample source:Brain | Species:Coho	Tank Replicate=B4; Experimental Replicate=1; RAW_FILE_NAME=164_AQ_B4_1_CO.mzML
SUBJECT_SAMPLE_FACTORS           	70	70_AQ_B4_1_CH	Treatment:Anthraquinone | Sample source:Brain | Species:Chinook	Tank Replicate=B4; Experimental Replicate=1; RAW_FILE_NAME=70_AQ_B4_1_CH.mzML
SUBJECT_SAMPLE_FACTORS           	236	236_6Q_B4_3_RT	Treatment:6PPD-quinone | Sample source:Brain | Species:Rainbow Trout	Tank Replicate=B4; Experimental Replicate=3; RAW_FILE_NAME=236_6Q_B4_3_RT.mzML
SUBJECT_SAMPLE_FACTORS           	171	171_6Q_B4_3_CO	Treatment:6PPD-quinone | Sample source:Brain | Species:Coho	Tank Replicate=B4; Experimental Replicate=3; RAW_FILE_NAME=171_6Q_B4_3_CO.mzML
SUBJECT_SAMPLE_FACTORS           	265	265_6Q_B4_3_CH	Treatment:6PPD-quinone | Sample source:Brain | Species:Chinook	Tank Replicate=B4; Experimental Replicate=3; RAW_FILE_NAME=265_6Q_B4_3_CH.mzML
SUBJECT_SAMPLE_FACTORS           	242	242_6Q_B4_2_RT	Treatment:6PPD-quinone | Sample source:Brain | Species:Rainbow Trout	Tank Replicate=B4; Experimental Replicate=2; RAW_FILE_NAME=242_6Q_B4_2_RT.mzML
SUBJECT_SAMPLE_FACTORS           	234	234_6Q_B4_2_CO	Treatment:6PPD-quinone | Sample source:Brain | Species:Coho	Tank Replicate=B4; Experimental Replicate=2; RAW_FILE_NAME=234_6Q_B4_2_CO.mzML
SUBJECT_SAMPLE_FACTORS           	194	194_6Q_B4_2_CH	Treatment:6PPD-quinone | Sample source:Brain | Species:Chinook	Tank Replicate=B4; Experimental Replicate=2; RAW_FILE_NAME=194_6Q_B4_2_CH.mzML
SUBJECT_SAMPLE_FACTORS           	138	138_6Q_B4_1_RT	Treatment:6PPD-quinone | Sample source:Brain | Species:Rainbow Trout	Tank Replicate=B4; Experimental Replicate=1; RAW_FILE_NAME=138_6Q_B4_1_RT.mzML
SUBJECT_SAMPLE_FACTORS           	111	111_6Q_B4_1_CO	Treatment:6PPD-quinone | Sample source:Brain | Species:Coho	Tank Replicate=B4; Experimental Replicate=1; RAW_FILE_NAME=111_6Q_B4_1_CO.mzML
SUBJECT_SAMPLE_FACTORS           	225	225_6Q_B4_1_CH	Treatment:6PPD-quinone | Sample source:Brain | Species:Chinook	Tank Replicate=B4; Experimental Replicate=1; RAW_FILE_NAME=225_6Q_B4_1_CH.mzML
SUBJECT_SAMPLE_FACTORS           	189	189_UC_B3_3_RT	Treatment:Unfed Control | Sample source:Brain | Species:Rainbow Trout	Tank Replicate=B3; Experimental Replicate=3; RAW_FILE_NAME=189_UC_B3_3_RT.mzML
SUBJECT_SAMPLE_FACTORS           	182	182_UC_B3_3_CO	Treatment:Unfed Control | Sample source:Brain | Species:Coho	Tank Replicate=B3; Experimental Replicate=3; RAW_FILE_NAME=182_UC_B3_3_CO.mzML
SUBJECT_SAMPLE_FACTORS           	159	159_UC_B3_3_CH	Treatment:Unfed Control | Sample source:Brain | Species:Chinook	Tank Replicate=B3; Experimental Replicate=3; RAW_FILE_NAME=159_UC_B3_3_CH.mzML
SUBJECT_SAMPLE_FACTORS           	252	252_UC_B3_2_RT	Treatment:Unfed Control | Sample source:Brain | Species:Rainbow Trout	Tank Replicate=B3; Experimental Replicate=2; RAW_FILE_NAME=252_UC_B3_2_RT.mzML
SUBJECT_SAMPLE_FACTORS           	156	156_UC_B3_2_CO	Treatment:Unfed Control | Sample source:Brain | Species:Coho	Tank Replicate=B3; Experimental Replicate=2; RAW_FILE_NAME=156_UC_B3_2_CO.mzML
SUBJECT_SAMPLE_FACTORS           	108	108_UC_B3_2_CH	Treatment:Unfed Control | Sample source:Brain | Species:Chinook	Tank Replicate=B3; Experimental Replicate=2; RAW_FILE_NAME=108_UC_B3_2_CH.mzML
SUBJECT_SAMPLE_FACTORS           	45	45_UC_B3_1_RT	Treatment:Unfed Control | Sample source:Brain | Species:Rainbow Trout	Tank Replicate=B3; Experimental Replicate=1; RAW_FILE_NAME=45_UC_B3_1_RT.mzML
SUBJECT_SAMPLE_FACTORS           	227	227_UC_B3_1_CO	Treatment:Unfed Control | Sample source:Brain | Species:Coho	Tank Replicate=B3; Experimental Replicate=1; RAW_FILE_NAME=227_UC_B3_1_CO.mzML
SUBJECT_SAMPLE_FACTORS           	176	176_UC_B3_1_CH	Treatment:Unfed Control | Sample source:Brain | Species:Chinook	Tank Replicate=B3; Experimental Replicate=1; RAW_FILE_NAME=176_UC_B3_1_CH.mzML
SUBJECT_SAMPLE_FACTORS           	29	29_IN_B3_3_RT	Treatment:Insult | Sample source:Brain | Species:Rainbow Trout	Tank Replicate=B3; Experimental Replicate=3; RAW_FILE_NAME=29_IN_B3_3_RT.mzML
SUBJECT_SAMPLE_FACTORS           	114	114_IN_B3_3_CO	Treatment:Insult | Sample source:Brain | Species:Coho	Tank Replicate=B3; Experimental Replicate=3; RAW_FILE_NAME=114_IN_B3_3_CO.mzML
SUBJECT_SAMPLE_FACTORS           	67	67_IN_B3_3_CH	Treatment:Insult | Sample source:Brain | Species:Chinook	Tank Replicate=B3; Experimental Replicate=3; RAW_FILE_NAME=67_IN_B3_3_CH.mzML
SUBJECT_SAMPLE_FACTORS           	166	166_IN_B3_2_RT	Treatment:Insult | Sample source:Brain | Species:Rainbow Trout	Tank Replicate=B3; Experimental Replicate=2; RAW_FILE_NAME=166_IN_B3_2_RT.mzML
SUBJECT_SAMPLE_FACTORS           	173	173_IN_B3_2_CO	Treatment:Insult | Sample source:Brain | Species:Coho	Tank Replicate=B3; Experimental Replicate=2; RAW_FILE_NAME=173_IN_B3_2_CO.mzML
SUBJECT_SAMPLE_FACTORS           	259	259_IN_B3_2_CH	Treatment:Insult | Sample source:Brain | Species:Chinook	Tank Replicate=B3; Experimental Replicate=2; RAW_FILE_NAME=259_IN_B3_2_CH.mzML
SUBJECT_SAMPLE_FACTORS           	200	200_IN_B3_1_RT	Treatment:Insult | Sample source:Brain | Species:Rainbow Trout	Tank Replicate=B3; Experimental Replicate=1; RAW_FILE_NAME=200_IN_B3_1_RT.mzML
SUBJECT_SAMPLE_FACTORS           	168	168_IN_B3_1_CO	Treatment:Insult | Sample source:Brain | Species:Coho	Tank Replicate=B3; Experimental Replicate=1; RAW_FILE_NAME=168_IN_B3_1_CO.mzML
SUBJECT_SAMPLE_FACTORS           	122	122_IN_B3_1_CH	Treatment:Insult | Sample source:Brain | Species:Chinook	Tank Replicate=B3; Experimental Replicate=1; RAW_FILE_NAME=122_IN_B3_1_CH.mzML
SUBJECT_SAMPLE_FACTORS           	202	202_FC_B3_3_RT	Treatment:Fed Control | Sample source:Brain | Species:Rainbow Trout	Tank Replicate=B3; Experimental Replicate=3; RAW_FILE_NAME=202_FC_B3_3_RT.mzML
SUBJECT_SAMPLE_FACTORS           	62	62_FC_B3_3_CO	Treatment:Fed Control | Sample source:Brain | Species:Coho	Tank Replicate=B3; Experimental Replicate=3; RAW_FILE_NAME=62_FC_B3_3_CO.mzML
SUBJECT_SAMPLE_FACTORS           	244	244_FC_B3_3_CH	Treatment:Fed Control | Sample source:Brain | Species:Chinook	Tank Replicate=B3; Experimental Replicate=3; RAW_FILE_NAME=244_FC_B3_3_CH.mzML
SUBJECT_SAMPLE_FACTORS           	3	3_FC_B3_2_RT	Treatment:Fed Control | Sample source:Brain | Species:Rainbow Trout	Tank Replicate=B3; Experimental Replicate=2; RAW_FILE_NAME=3_FC_B3_2_RT.mzML
SUBJECT_SAMPLE_FACTORS           	4	4_FC_B3_2_CO	Treatment:Fed Control | Sample source:Brain | Species:Coho	Tank Replicate=B3; Experimental Replicate=2; RAW_FILE_NAME=4_FC_B3_2_CO.mzML
SUBJECT_SAMPLE_FACTORS           	135	135_FC_B3_2_CH	Treatment:Fed Control | Sample source:Brain | Species:Chinook	Tank Replicate=B3; Experimental Replicate=2; RAW_FILE_NAME=135_FC_B3_2_CH.mzML
SUBJECT_SAMPLE_FACTORS           	145	145_FC_B3_1_RT	Treatment:Fed Control | Sample source:Brain | Species:Rainbow Trout	Tank Replicate=B3; Experimental Replicate=1; RAW_FILE_NAME=145_FC_B3_1_RT.mzML
SUBJECT_SAMPLE_FACTORS           	25	25_FC_B3_1_CO	Treatment:Fed Control | Sample source:Brain | Species:Coho	Tank Replicate=B3; Experimental Replicate=1; RAW_FILE_NAME=25_FC_B3_1_CO.mzML
SUBJECT_SAMPLE_FACTORS           	33	33_FC_B3_1_CH	Treatment:Fed Control | Sample source:Brain | Species:Chinook	Tank Replicate=B3; Experimental Replicate=1; RAW_FILE_NAME=33_FC_B3_1_CH.mzML
SUBJECT_SAMPLE_FACTORS           	48	48_CE_B3_3_RT	Treatment:Coexposure | Sample source:Brain | Species:Rainbow Trout	Tank Replicate=B3; Experimental Replicate=3; RAW_FILE_NAME=48_CE_B3_3_RT.mzML
SUBJECT_SAMPLE_FACTORS           	2	2_CE_B3_3_CO	Treatment:Coexposure | Sample source:Brain | Species:Coho	Tank Replicate=B3; Experimental Replicate=3; RAW_FILE_NAME=2_CE_B3_3_CO.mzML
SUBJECT_SAMPLE_FACTORS           	262	262_CE_B3_3_CH	Treatment:Coexposure | Sample source:Brain | Species:Chinook	Tank Replicate=B3; Experimental Replicate=3; RAW_FILE_NAME=262_CE_B3_3_CH.mzML
SUBJECT_SAMPLE_FACTORS           	238	238_CE_B3_2_RT	Treatment:Coexposure | Sample source:Brain | Species:Rainbow Trout	Tank Replicate=B3; Experimental Replicate=2; RAW_FILE_NAME=238_CE_B3_2_RT.mzML
SUBJECT_SAMPLE_FACTORS           	47	47_CE_B3_2_CO	Treatment:Coexposure | Sample source:Brain | Species:Coho	Tank Replicate=B3; Experimental Replicate=2; RAW_FILE_NAME=47_CE_B3_2_CO.mzML
SUBJECT_SAMPLE_FACTORS           	103	103_CE_B3_2_CH	Treatment:Coexposure | Sample source:Brain | Species:Chinook	Tank Replicate=B3; Experimental Replicate=2; RAW_FILE_NAME=103_CE_B3_2_CH.mzML
SUBJECT_SAMPLE_FACTORS           	224	224_CE_B3_1_RT	Treatment:Coexposure | Sample source:Brain | Species:Rainbow Trout	Tank Replicate=B3; Experimental Replicate=1; RAW_FILE_NAME=224_CE_B3_1_RT.mzML
SUBJECT_SAMPLE_FACTORS           	247	247_CE_B3_1_CO	Treatment:Coexposure | Sample source:Brain | Species:Coho	Tank Replicate=B3; Experimental Replicate=1; RAW_FILE_NAME=247_CE_B3_1_CO.mzML
SUBJECT_SAMPLE_FACTORS           	199	199_CE_B3_1_CH	Treatment:Coexposure | Sample source:Brain | Species:Chinook	Tank Replicate=B3; Experimental Replicate=1; RAW_FILE_NAME=199_CE_B3_1_CH.mzML
SUBJECT_SAMPLE_FACTORS           	147	147_AQ_B3_3_RT	Treatment:Anthraquinone | Sample source:Brain | Species:Rainbow Trout	Tank Replicate=B3; Experimental Replicate=3; RAW_FILE_NAME=147_AQ_B3_3_RT.mzML
SUBJECT_SAMPLE_FACTORS           	28	28_AQ_B3_3_CO	Treatment:Anthraquinone | Sample source:Brain | Species:Coho	Tank Replicate=B3; Experimental Replicate=3; RAW_FILE_NAME=28_AQ_B3_3_CO.mzML
SUBJECT_SAMPLE_FACTORS           	57	57_AQ_B3_3_CH	Treatment:Anthraquinone | Sample source:Brain | Species:Chinook	Tank Replicate=B3; Experimental Replicate=3; RAW_FILE_NAME=57_AQ_B3_3_CH.mzML
SUBJECT_SAMPLE_FACTORS           	245	245_AQ_B3_2_RT	Treatment:Anthraquinone | Sample source:Brain | Species:Rainbow Trout	Tank Replicate=B3; Experimental Replicate=2; RAW_FILE_NAME=245_AQ_B3_2_RT.mzML
SUBJECT_SAMPLE_FACTORS           	30	30_AQ_B3_2_CH	Treatment:Anthraquinone | Sample source:Brain | Species:Chinook	Tank Replicate=B3; Experimental Replicate=2; RAW_FILE_NAME=30_AQ_B3_2_CH.mzML
SUBJECT_SAMPLE_FACTORS           	42	42_AQ_B3_1_CO	Treatment:Anthraquinone | Sample source:Brain | Species:Coho	Tank Replicate=B3; Experimental Replicate=1; RAW_FILE_NAME=42_AQ_B3_1_CO.mzML
SUBJECT_SAMPLE_FACTORS           	151	151_6Q_B3_3_RT	Treatment:6PPD-quinone | Sample source:Brain | Species:Rainbow Trout	Tank Replicate=B3; Experimental Replicate=3; RAW_FILE_NAME=151_6Q_B3_3_RT.mzML
SUBJECT_SAMPLE_FACTORS           	175	175_6Q_B3_3_CO	Treatment:6PPD-quinone | Sample source:Brain | Species:Coho	Tank Replicate=B3; Experimental Replicate=3; RAW_FILE_NAME=175_6Q_B3_3_CO.mzML
SUBJECT_SAMPLE_FACTORS           	230	230_6Q_B3_3_CH	Treatment:6PPD-quinone | Sample source:Brain | Species:Chinook	Tank Replicate=B3; Experimental Replicate=3; RAW_FILE_NAME=230_6Q_B3_3_CH.mzML
SUBJECT_SAMPLE_FACTORS           	88	88_6Q_B3_2_CO	Treatment:6PPD-quinone | Sample source:Brain | Species:Coho	Tank Replicate=B3; Experimental Replicate=2; RAW_FILE_NAME=88_6Q_B3_2_CO.mzML
SUBJECT_SAMPLE_FACTORS           	11	11_6Q_B3_2_CH	Treatment:6PPD-quinone | Sample source:Brain | Species:Chinook	Tank Replicate=B3; Experimental Replicate=2; RAW_FILE_NAME=11_6Q_B3_2_CH.mzML
SUBJECT_SAMPLE_FACTORS           	197	197_6Q_B3_1_RT	Treatment:6PPD-quinone | Sample source:Brain | Species:Rainbow Trout	Tank Replicate=B3; Experimental Replicate=1; RAW_FILE_NAME=197_6Q_B3_1_RT.mzML
SUBJECT_SAMPLE_FACTORS           	250	250_6Q_B3_1_CO	Treatment:6PPD-quinone | Sample source:Brain | Species:Coho	Tank Replicate=B3; Experimental Replicate=1; RAW_FILE_NAME=250_6Q_B3_1_CO.mzML
SUBJECT_SAMPLE_FACTORS           	161	161_6Q_B3_1_CH	Treatment:6PPD-quinone | Sample source:Brain | Species:Chinook	Tank Replicate=B3; Experimental Replicate=1; RAW_FILE_NAME=161_6Q_B3_1_CH.mzML
SUBJECT_SAMPLE_FACTORS           	232	232_UC_B2_3_RT	Treatment:Unfed Control | Sample source:Brain | Species:Rainbow Trout	Tank Replicate=B2; Experimental Replicate=3; RAW_FILE_NAME=232_UC_B2_3_RT.mzML
SUBJECT_SAMPLE_FACTORS           	75	75_UC_B2_3_CO	Treatment:Unfed Control | Sample source:Brain | Species:Coho	Tank Replicate=B2; Experimental Replicate=3; RAW_FILE_NAME=75_UC_B2_3_CO.mzML
SUBJECT_SAMPLE_FACTORS           	93	93_UC_B2_3_CH	Treatment:Unfed Control | Sample source:Brain | Species:Chinook	Tank Replicate=B2; Experimental Replicate=3; RAW_FILE_NAME=93_UC_B2_3_CH.mzML
SUBJECT_SAMPLE_FACTORS           	269	269_UC_B2_2_RT	Treatment:Unfed Control | Sample source:Brain | Species:Rainbow Trout	Tank Replicate=B2; Experimental Replicate=2; RAW_FILE_NAME=269_UC_B2_2_RT.mzML
SUBJECT_SAMPLE_FACTORS           	98	98_UC_B2_2_CO	Treatment:Unfed Control | Sample source:Brain | Species:Coho	Tank Replicate=B2; Experimental Replicate=2; RAW_FILE_NAME=98_UC_B2_2_CO.mzML
SUBJECT_SAMPLE_FACTORS           	153	153_UC_B2_2_CH	Treatment:Unfed Control | Sample source:Brain | Species:Chinook	Tank Replicate=B2; Experimental Replicate=2; RAW_FILE_NAME=153_UC_B2_2_CH.mzML
SUBJECT_SAMPLE_FACTORS           	198	198_UC_B2_1_RT	Treatment:Unfed Control | Sample source:Brain | Species:Rainbow Trout	Tank Replicate=B2; Experimental Replicate=1; RAW_FILE_NAME=198_UC_B2_1_RT.mzML
SUBJECT_SAMPLE_FACTORS           	209	209_UC_B2_1_CO	Treatment:Unfed Control | Sample source:Brain | Species:Coho	Tank Replicate=B2; Experimental Replicate=1; RAW_FILE_NAME=209_UC_B2_1_CO.mzML
SUBJECT_SAMPLE_FACTORS           	55	55_UC_B2_1_CH	Treatment:Unfed Control | Sample source:Brain | Species:Chinook	Tank Replicate=B2; Experimental Replicate=1; RAW_FILE_NAME=55_UC_B2_1_CH.mzML
SUBJECT_SAMPLE_FACTORS           	63	63_IN_B2_3_RT	Treatment:Insult | Sample source:Brain | Species:Rainbow Trout	Tank Replicate=B2; Experimental Replicate=3; RAW_FILE_NAME=63_IN_B2_3_RT.mzML
SUBJECT_SAMPLE_FACTORS           	229	229_IN_B2_3_CO	Treatment:Insult | Sample source:Brain | Species:Coho	Tank Replicate=B2; Experimental Replicate=3; RAW_FILE_NAME=229_IN_B2_3_CO.mzML
SUBJECT_SAMPLE_FACTORS           	125	125_IN_B2_3_CH	Treatment:Insult | Sample source:Brain | Species:Chinook	Tank Replicate=B2; Experimental Replicate=3; RAW_FILE_NAME=125_IN_B2_3_CH.mzML
SUBJECT_SAMPLE_FACTORS           	203	203_IN_B2_2_RT	Treatment:Insult | Sample source:Brain | Species:Rainbow Trout	Tank Replicate=B2; Experimental Replicate=2; RAW_FILE_NAME=203_IN_B2_2_RT.mzML
SUBJECT_SAMPLE_FACTORS           	96	96_IN_B2_2_CO	Treatment:Insult | Sample source:Brain | Species:Coho	Tank Replicate=B2; Experimental Replicate=2; RAW_FILE_NAME=96_IN_B2_2_CO.mzML
SUBJECT_SAMPLE_FACTORS           	92	92_IN_B2_2_CH	Treatment:Insult | Sample source:Brain | Species:Chinook	Tank Replicate=B2; Experimental Replicate=2; RAW_FILE_NAME=92_IN_B2_2_CH.mzML
SUBJECT_SAMPLE_FACTORS           	10	10_IN_B2_1_RT	Treatment:Insult | Sample source:Brain | Species:Rainbow Trout	Tank Replicate=B2; Experimental Replicate=1; RAW_FILE_NAME=10_IN_B2_1_RT.mzML
SUBJECT_SAMPLE_FACTORS           	204	204_IN_B2_1_CO	Treatment:Insult | Sample source:Brain | Species:Coho	Tank Replicate=B2; Experimental Replicate=1; RAW_FILE_NAME=204_IN_B2_1_CO.mzML
SUBJECT_SAMPLE_FACTORS           	20	20_IN_B2_1_CH	Treatment:Insult | Sample source:Brain | Species:Chinook	Tank Replicate=B2; Experimental Replicate=1; RAW_FILE_NAME=20_IN_B2_1_CH.mzML
SUBJECT_SAMPLE_FACTORS           	254	254_FC_B2_3_RT	Treatment:Fed Control | Sample source:Brain | Species:Rainbow Trout	Tank Replicate=B2; Experimental Replicate=3; RAW_FILE_NAME=254_FC_B2_3_RT.mzML
SUBJECT_SAMPLE_FACTORS           	251	251_FC_B2_3_CO	Treatment:Fed Control | Sample source:Brain | Species:Coho	Tank Replicate=B2; Experimental Replicate=3; RAW_FILE_NAME=251_FC_B2_3_CO.mzML
SUBJECT_SAMPLE_FACTORS           	180	180_FC_B2_3_CH	Treatment:Fed Control | Sample source:Brain | Species:Chinook	Tank Replicate=B2; Experimental Replicate=3; RAW_FILE_NAME=180_FC_B2_3_CH.mzML
SUBJECT_SAMPLE_FACTORS           	52	52_FC_B2_2_RT	Treatment:Fed Control | Sample source:Brain | Species:Rainbow Trout	Tank Replicate=B2; Experimental Replicate=2; RAW_FILE_NAME=52_FC_B2_2_RT.mzML
SUBJECT_SAMPLE_FACTORS           	208	208_FC_B2_2_CO	Treatment:Fed Control | Sample source:Brain | Species:Coho	Tank Replicate=B2; Experimental Replicate=2; RAW_FILE_NAME=208_FC_B2_2_CO.mzML
SUBJECT_SAMPLE_FACTORS           	130	130_FC_B2_2_CH	Treatment:Fed Control | Sample source:Brain | Species:Chinook	Tank Replicate=B2; Experimental Replicate=2; RAW_FILE_NAME=130_FC_B2_2_CH.mzML
SUBJECT_SAMPLE_FACTORS           	5	5_FC_B2_1_RT	Treatment:Fed Control | Sample source:Brain | Species:Rainbow Trout	Tank Replicate=B2; Experimental Replicate=1; RAW_FILE_NAME=5_FC_B2_1_RT.mzML
SUBJECT_SAMPLE_FACTORS           	268	268_FC_B2_1_CO	Treatment:Fed Control | Sample source:Brain | Species:Coho	Tank Replicate=B2; Experimental Replicate=1; RAW_FILE_NAME=268_FC_B2_1_CO.mzML
SUBJECT_SAMPLE_FACTORS           	115	115_FC_B2_1_CH	Treatment:Fed Control | Sample source:Brain | Species:Chinook	Tank Replicate=B2; Experimental Replicate=1; RAW_FILE_NAME=115_FC_B2_1_CH.mzML
SUBJECT_SAMPLE_FACTORS           	206	206_CE_B2_3_RT	Treatment:Coexposure | Sample source:Brain | Species:Rainbow Trout	Tank Replicate=B2; Experimental Replicate=3; RAW_FILE_NAME=206_CE_B2_3_RT.mzML
SUBJECT_SAMPLE_FACTORS           	105	105_CE_B2_3_CO	Treatment:Coexposure | Sample source:Brain | Species:Coho	Tank Replicate=B2; Experimental Replicate=3; RAW_FILE_NAME=105_CE_B2_3_CO.mzML
SUBJECT_SAMPLE_FACTORS           	37	37_CE_B2_3_CH	Treatment:Coexposure | Sample source:Brain | Species:Chinook	Tank Replicate=B2; Experimental Replicate=3; RAW_FILE_NAME=37_CE_B2_3_CH.mzML
SUBJECT_SAMPLE_FACTORS           	73	73_CE_B2_2_RT	Treatment:Coexposure | Sample source:Brain | Species:Rainbow Trout	Tank Replicate=B2; Experimental Replicate=2; RAW_FILE_NAME=73_CE_B2_2_RT.mzML
SUBJECT_SAMPLE_FACTORS           	74	74_CE_B2_2_CO	Treatment:Coexposure | Sample source:Brain | Species:Coho	Tank Replicate=B2; Experimental Replicate=2; RAW_FILE_NAME=74_CE_B2_2_CO.mzML
SUBJECT_SAMPLE_FACTORS           	21	21_CE_B2_2_CH	Treatment:Coexposure | Sample source:Brain | Species:Chinook	Tank Replicate=B2; Experimental Replicate=2; RAW_FILE_NAME=21_CE_B2_2_CH.mzML
SUBJECT_SAMPLE_FACTORS           	15	15_CE_B2_1_RT	Treatment:Coexposure | Sample source:Brain | Species:Rainbow Trout	Tank Replicate=B2; Experimental Replicate=1; RAW_FILE_NAME=15_CE_B2_1_RT.mzML
SUBJECT_SAMPLE_FACTORS           	41	41_CE_B2_1_CO	Treatment:Coexposure | Sample source:Brain | Species:Coho	Tank Replicate=B2; Experimental Replicate=1; RAW_FILE_NAME=41_CE_B2_1_CO.mzML
SUBJECT_SAMPLE_FACTORS           	89	89_CE_B2_1_CH	Treatment:Coexposure | Sample source:Brain | Species:Chinook	Tank Replicate=B2; Experimental Replicate=1; RAW_FILE_NAME=89_CE_B2_1_CH.mzML
SUBJECT_SAMPLE_FACTORS           	192	192_AQ_B2_3_RT	Treatment:Anthraquinone | Sample source:Brain | Species:Rainbow Trout	Tank Replicate=B2; Experimental Replicate=3; RAW_FILE_NAME=192_AQ_B2_3_RT.mzML
SUBJECT_SAMPLE_FACTORS           	131	131_AQ_B2_3_CO	Treatment:Anthraquinone | Sample source:Brain | Species:Coho	Tank Replicate=B2; Experimental Replicate=3; RAW_FILE_NAME=131_AQ_B2_3_CO.mzML
SUBJECT_SAMPLE_FACTORS           	148	148_AQ_B2_3_CH	Treatment:Anthraquinone | Sample source:Brain | Species:Chinook	Tank Replicate=B2; Experimental Replicate=3; RAW_FILE_NAME=148_AQ_B2_3_CH.mzML
SUBJECT_SAMPLE_FACTORS           	79	79_AQ_B2_2_RT	Treatment:Anthraquinone | Sample source:Brain | Species:Rainbow Trout	Tank Replicate=B2; Experimental Replicate=2; RAW_FILE_NAME=79_AQ_B2_2_RT.mzML
SUBJECT_SAMPLE_FACTORS           	56	56_AQ_B2_2_CO	Treatment:Anthraquinone | Sample source:Brain | Species:Coho	Tank Replicate=B2; Experimental Replicate=2; RAW_FILE_NAME=56_AQ_B2_2_CO.mzML
SUBJECT_SAMPLE_FACTORS           	235	235_AQ_B2_2_CH	Treatment:Anthraquinone | Sample source:Brain | Species:Chinook	Tank Replicate=B2; Experimental Replicate=2; RAW_FILE_NAME=235_AQ_B2_2_CH.mzML
SUBJECT_SAMPLE_FACTORS           	143	143_AQ_B2_1_RT	Treatment:Anthraquinone | Sample source:Brain | Species:Rainbow Trout	Tank Replicate=B2; Experimental Replicate=1; RAW_FILE_NAME=143_AQ_B2_1_RT.mzML
SUBJECT_SAMPLE_FACTORS           	65	65_AQ_B2_1_CO	Treatment:Anthraquinone | Sample source:Brain | Species:Coho	Tank Replicate=B2; Experimental Replicate=1; RAW_FILE_NAME=65_AQ_B2_1_CO.mzML
SUBJECT_SAMPLE_FACTORS           	117	117_AQ_B2_1_CH	Treatment:Anthraquinone | Sample source:Brain | Species:Chinook	Tank Replicate=B2; Experimental Replicate=1; RAW_FILE_NAME=117_AQ_B2_1_CH.mzML
SUBJECT_SAMPLE_FACTORS           	19	19_6Q_B2_3_RT	Treatment:6PPD-quinone | Sample source:Brain | Species:Rainbow Trout	Tank Replicate=B2; Experimental Replicate=3; RAW_FILE_NAME=19_6Q_B2_3_RT.mzML
SUBJECT_SAMPLE_FACTORS           	158	158_6Q_B2_3_CO	Treatment:6PPD-quinone | Sample source:Brain | Species:Coho	Tank Replicate=B2; Experimental Replicate=3; RAW_FILE_NAME=158_6Q_B2_3_CO.mzML
SUBJECT_SAMPLE_FACTORS           	38	38_6Q_B2_3_CH	Treatment:6PPD-quinone | Sample source:Brain | Species:Chinook	Tank Replicate=B2; Experimental Replicate=3; RAW_FILE_NAME=38_6Q_B2_3_CH.mzML
SUBJECT_SAMPLE_FACTORS           	127	127_6Q_B2_2_RT	Treatment:6PPD-quinone | Sample source:Brain | Species:Rainbow Trout	Tank Replicate=B2; Experimental Replicate=2; RAW_FILE_NAME=127_6Q_B2_2_RT.mzML
SUBJECT_SAMPLE_FACTORS           	240	240_6Q_B2_2_CO	Treatment:6PPD-quinone | Sample source:Brain | Species:Coho	Tank Replicate=B2; Experimental Replicate=2; RAW_FILE_NAME=240_6Q_B2_2_CO.mzML
SUBJECT_SAMPLE_FACTORS           	253	253_6Q_B2_1_RT	Treatment:6PPD-quinone | Sample source:Brain | Species:Rainbow Trout	Tank Replicate=B2; Experimental Replicate=1; RAW_FILE_NAME=253_6Q_B2_1_RT.mzML
SUBJECT_SAMPLE_FACTORS           	8	8_6Q_B2_1_CO	Treatment:6PPD-quinone | Sample source:Brain | Species:Coho	Tank Replicate=B2; Experimental Replicate=1; RAW_FILE_NAME=8_6Q_B2_1_CO.mzML
SUBJECT_SAMPLE_FACTORS           	133	133_6Q_B2_1_CH	Treatment:6PPD-quinone | Sample source:Brain | Species:Chinook	Tank Replicate=B2; Experimental Replicate=1; RAW_FILE_NAME=133_6Q_B2_1_CH.mzML
SUBJECT_SAMPLE_FACTORS           	71	71_UC_B1_3_RT	Treatment:Unfed Control | Sample source:Brain | Species:Rainbow Trout	Tank Replicate=B1; Experimental Replicate=3; RAW_FILE_NAME=71_UC_B1_3_RT.mzML
SUBJECT_SAMPLE_FACTORS           	196	196_UC_B1_3_CO	Treatment:Unfed Control | Sample source:Brain | Species:Coho	Tank Replicate=B1; Experimental Replicate=3; RAW_FILE_NAME=196_UC_B1_3_CO.mzML
SUBJECT_SAMPLE_FACTORS           	113	113_UC_B1_3_CH	Treatment:Unfed Control | Sample source:Brain | Species:Chinook	Tank Replicate=B1; Experimental Replicate=3; RAW_FILE_NAME=113_UC_B1_3_CH.mzML
SUBJECT_SAMPLE_FACTORS           	43	43_UC_B1_2_RT	Treatment:Unfed Control | Sample source:Brain | Species:Rainbow Trout	Tank Replicate=B1; Experimental Replicate=2; RAW_FILE_NAME=43_UC_B1_2_RT.mzML
SUBJECT_SAMPLE_FACTORS           	255	255_UC_B1_2_CO	Treatment:Unfed Control | Sample source:Brain | Species:Coho	Tank Replicate=B1; Experimental Replicate=2; RAW_FILE_NAME=255_UC_B1_2_CO.mzML
SUBJECT_SAMPLE_FACTORS           	267	267_UC_B1_2_CH	Treatment:Unfed Control | Sample source:Brain | Species:Chinook	Tank Replicate=B1; Experimental Replicate=2; RAW_FILE_NAME=267_UC_B1_2_CH.mzML
SUBJECT_SAMPLE_FACTORS           	85	85_UC_B1_1_RT	Treatment:Unfed Control | Sample source:Brain | Species:Rainbow Trout	Tank Replicate=B1; Experimental Replicate=1; RAW_FILE_NAME=85_UC_B1_1_RT.mzML
SUBJECT_SAMPLE_FACTORS           	7	7_UC_B1_1_CO	Treatment:Unfed Control | Sample source:Brain | Species:Coho	Tank Replicate=B1; Experimental Replicate=1; RAW_FILE_NAME=7_UC_B1_1_CO.mzML
SUBJECT_SAMPLE_FACTORS           	102	102_UC_B1_1_CH	Treatment:Unfed Control | Sample source:Brain | Species:Chinook	Tank Replicate=B1; Experimental Replicate=1; RAW_FILE_NAME=102_UC_B1_1_CH.mzML
SUBJECT_SAMPLE_FACTORS           	31	31_IN_B1_3_RT	Treatment:Insult | Sample source:Brain | Species:Rainbow Trout	Tank Replicate=B1; Experimental Replicate=3; RAW_FILE_NAME=31_IN_B1_3_RT.mzML
SUBJECT_SAMPLE_FACTORS           	140	140_IN_B1_3_CO	Treatment:Insult | Sample source:Brain | Species:Coho	Tank Replicate=B1; Experimental Replicate=3; RAW_FILE_NAME=140_IN_B1_3_CO.mzML
SUBJECT_SAMPLE_FACTORS           	27	27_IN_B1_3_CH	Treatment:Insult | Sample source:Brain | Species:Chinook	Tank Replicate=B1; Experimental Replicate=3; RAW_FILE_NAME=27_IN_B1_3_CH.mzML
SUBJECT_SAMPLE_FACTORS           	80	80_IN_B1_2_RT	Treatment:Insult | Sample source:Brain | Species:Rainbow Trout	Tank Replicate=B1; Experimental Replicate=2; RAW_FILE_NAME=80_IN_B1_2_RT.mzML
SUBJECT_SAMPLE_FACTORS           	87	87_IN_B1_2_CO	Treatment:Insult | Sample source:Brain | Species:Coho	Tank Replicate=B1; Experimental Replicate=2; RAW_FILE_NAME=87_IN_B1_2_CO.mzML
SUBJECT_SAMPLE_FACTORS           	179	179_IN_B1_2_CH	Treatment:Insult | Sample source:Brain | Species:Chinook	Tank Replicate=B1; Experimental Replicate=2; RAW_FILE_NAME=179_IN_B1_2_CH.mzML
SUBJECT_SAMPLE_FACTORS           	258	258_IN_B1_1_RT	Treatment:Insult | Sample source:Brain | Species:Rainbow Trout	Tank Replicate=B1; Experimental Replicate=1; RAW_FILE_NAME=258_IN_B1_1_RT.mzML
SUBJECT_SAMPLE_FACTORS           	91	91_IN_B1_1_CO	Treatment:Insult | Sample source:Brain | Species:Coho	Tank Replicate=B1; Experimental Replicate=1; RAW_FILE_NAME=91_IN_B1_1_CO.mzML
SUBJECT_SAMPLE_FACTORS           	107	107_IN_B1_1_CH	Treatment:Insult | Sample source:Brain | Species:Chinook	Tank Replicate=B1; Experimental Replicate=1; RAW_FILE_NAME=107_IN_B1_1_CH.mzML
SUBJECT_SAMPLE_FACTORS           	137	137_FC_B1_3_RT	Treatment:Fed Control | Sample source:Brain | Species:Rainbow Trout	Tank Replicate=B1; Experimental Replicate=3; RAW_FILE_NAME=137_FC_B1_3_RT.mzML
SUBJECT_SAMPLE_FACTORS           	83	83_FC_B1_3_CO	Treatment:Fed Control | Sample source:Brain | Species:Coho	Tank Replicate=B1; Experimental Replicate=3; RAW_FILE_NAME=83_FC_B1_3_CO.mzML
SUBJECT_SAMPLE_FACTORS           	61	61_FC_B1_3_CH	Treatment:Fed Control | Sample source:Brain | Species:Chinook	Tank Replicate=B1; Experimental Replicate=3; RAW_FILE_NAME=61_FC_B1_3_CH.mzML
SUBJECT_SAMPLE_FACTORS           	39	39_FC_B1_2_RT	Treatment:Fed Control | Sample source:Brain | Species:Rainbow Trout	Tank Replicate=B1; Experimental Replicate=2; RAW_FILE_NAME=39_FC_B1_2_RT.mzML
SUBJECT_SAMPLE_FACTORS           	84	84_FC_B1_2_CO	Treatment:Fed Control | Sample source:Brain | Species:Coho	Tank Replicate=B1; Experimental Replicate=2; RAW_FILE_NAME=84_FC_B1_2_CO.mzML
SUBJECT_SAMPLE_FACTORS           	207	207_FC_B1_2_CH	Treatment:Fed Control | Sample source:Brain | Species:Chinook	Tank Replicate=B1; Experimental Replicate=2; RAW_FILE_NAME=207_FC_B1_2_CH.mzML
SUBJECT_SAMPLE_FACTORS           	190	190_FC_B1_1_RT	Treatment:Fed Control | Sample source:Brain | Species:Rainbow Trout	Tank Replicate=B1; Experimental Replicate=1; RAW_FILE_NAME=190_FC_B1_1_RT.mzML
SUBJECT_SAMPLE_FACTORS           	248	248_FC_B1_1_CO	Treatment:Fed Control | Sample source:Brain | Species:Coho	Tank Replicate=B1; Experimental Replicate=1; RAW_FILE_NAME=248_FC_B1_1_CO.mzML
SUBJECT_SAMPLE_FACTORS           	116	116_FC_B1_1_CH	Treatment:Fed Control | Sample source:Brain | Species:Chinook	Tank Replicate=B1; Experimental Replicate=1; RAW_FILE_NAME=116_FC_B1_1_CH.mzML
SUBJECT_SAMPLE_FACTORS           	90	90_CE_B1_3_RT	Treatment:Coexposure | Sample source:Brain | Species:Rainbow Trout	Tank Replicate=B1; Experimental Replicate=3; RAW_FILE_NAME=90_CE_B1_3_RT.mzML
SUBJECT_SAMPLE_FACTORS           	266	266_CE_B1_3_CO	Treatment:Coexposure | Sample source:Brain | Species:Coho	Tank Replicate=B1; Experimental Replicate=3; RAW_FILE_NAME=266_CE_B1_3_CO.mzML
SUBJECT_SAMPLE_FACTORS           	239	239_CE_B1_3_CH	Treatment:Coexposure | Sample source:Brain | Species:Chinook	Tank Replicate=B1; Experimental Replicate=3; RAW_FILE_NAME=239_CE_B1_3_CH.mzML
SUBJECT_SAMPLE_FACTORS           	139	139_CE_B1_2_RT	Treatment:Coexposure | Sample source:Brain | Species:Rainbow Trout	Tank Replicate=B1; Experimental Replicate=2; RAW_FILE_NAME=139_CE_B1_2_RT.mzML
SUBJECT_SAMPLE_FACTORS           	160	160_CE_B1_2_CO	Treatment:Coexposure | Sample source:Brain | Species:Coho	Tank Replicate=B1; Experimental Replicate=2; RAW_FILE_NAME=160_CE_B1_2_CO.mzML
SUBJECT_SAMPLE_FACTORS           	222	222_CE_B1_2_CH	Treatment:Coexposure | Sample source:Brain | Species:Chinook	Tank Replicate=B1; Experimental Replicate=2; RAW_FILE_NAME=222_CE_B1_2_CH.mzML
SUBJECT_SAMPLE_FACTORS           	69	69_CE_B1_1_RT	Treatment:Coexposure | Sample source:Brain | Species:Rainbow Trout	Tank Replicate=B1; Experimental Replicate=1; RAW_FILE_NAME=69_CE_B1_1_RT.mzML
SUBJECT_SAMPLE_FACTORS           	144	144_CE_B1_1_CO	Treatment:Coexposure | Sample source:Brain | Species:Coho	Tank Replicate=B1; Experimental Replicate=1; RAW_FILE_NAME=144_CE_B1_1_CO.mzML
SUBJECT_SAMPLE_FACTORS           	54	54_CE_B1_1_CH	Treatment:Coexposure | Sample source:Brain | Species:Chinook	Tank Replicate=B1; Experimental Replicate=1; RAW_FILE_NAME=54_CE_B1_1_CH.mzML
SUBJECT_SAMPLE_FACTORS           	50	50_AQ_B1_3_RT	Treatment:Anthraquinone | Sample source:Brain | Species:Rainbow Trout	Tank Replicate=B1; Experimental Replicate=3; RAW_FILE_NAME=50_AQ_B1_3_RT.mzML
SUBJECT_SAMPLE_FACTORS           	134	134_AQ_B1_3_CO	Treatment:Anthraquinone | Sample source:Brain | Species:Coho	Tank Replicate=B1; Experimental Replicate=3; RAW_FILE_NAME=134_AQ_B1_3_CO.mzML
SUBJECT_SAMPLE_FACTORS           	185	185_AQ_B1_3_CH	Treatment:Anthraquinone | Sample source:Brain | Species:Chinook	Tank Replicate=B1; Experimental Replicate=3; RAW_FILE_NAME=185_AQ_B1_3_CH.mzML
SUBJECT_SAMPLE_FACTORS           	249	249_AQ_B1_2_RT	Treatment:Anthraquinone | Sample source:Brain | Species:Rainbow Trout	Tank Replicate=B1; Experimental Replicate=2; RAW_FILE_NAME=249_AQ_B1_2_RT.mzML
SUBJECT_SAMPLE_FACTORS           	97	97_AQ_B1_2_CO	Treatment:Anthraquinone | Sample source:Brain | Species:Coho	Tank Replicate=B1; Experimental Replicate=2; RAW_FILE_NAME=97_AQ_B1_2_CO.mzML
SUBJECT_SAMPLE_FACTORS           	101	101_AQ_B1_2_CH	Treatment:Anthraquinone | Sample source:Brain | Species:Chinook	Tank Replicate=B1; Experimental Replicate=2; RAW_FILE_NAME=101_AQ_B1_2_CH.mzML
SUBJECT_SAMPLE_FACTORS           	81	81_AQ_B1_1_RT	Treatment:Anthraquinone | Sample source:Brain | Species:Rainbow Trout	Tank Replicate=B1; Experimental Replicate=1; RAW_FILE_NAME=81_AQ_B1_1_RT.mzML
SUBJECT_SAMPLE_FACTORS           	155	155_AQ_B1_1_CO	Treatment:Anthraquinone | Sample source:Brain | Species:Coho	Tank Replicate=B1; Experimental Replicate=1; RAW_FILE_NAME=155_AQ_B1_1_CO.mzML
SUBJECT_SAMPLE_FACTORS           	51	51_AQ_B1_1_CH	Treatment:Anthraquinone | Sample source:Brain | Species:Chinook	Tank Replicate=B1; Experimental Replicate=1; RAW_FILE_NAME=51_AQ_B1_1_CH.mzML
SUBJECT_SAMPLE_FACTORS           	146	146_6Q_B1_3_RT	Treatment:6PPD-quinone | Sample source:Brain | Species:Rainbow Trout	Tank Replicate=B1; Experimental Replicate=3; RAW_FILE_NAME=146_6Q_B1_3_RT.mzML
SUBJECT_SAMPLE_FACTORS           	169	169_6Q_B1_3_CO	Treatment:6PPD-quinone | Sample source:Brain | Species:Coho	Tank Replicate=B1; Experimental Replicate=3; RAW_FILE_NAME=169_6Q_B1_3_CO.mzML
SUBJECT_SAMPLE_FACTORS           	119	119_6Q_B1_3_CH	Treatment:6PPD-quinone | Sample source:Brain | Species:Chinook	Tank Replicate=B1; Experimental Replicate=3; RAW_FILE_NAME=119_6Q_B1_3_CH.mzML
SUBJECT_SAMPLE_FACTORS           	188	188_6Q_B1_2_RT	Treatment:6PPD-quinone | Sample source:Brain | Species:Rainbow Trout	Tank Replicate=B1; Experimental Replicate=2; RAW_FILE_NAME=188_6Q_B1_2_RT.mzML
SUBJECT_SAMPLE_FACTORS           	261	261_6Q_B1_2_CO	Treatment:6PPD-quinone | Sample source:Brain | Species:Coho	Tank Replicate=B1; Experimental Replicate=2; RAW_FILE_NAME=261_6Q_B1_2_CO.mzML
SUBJECT_SAMPLE_FACTORS           	142	142_6Q_B1_2_CH	Treatment:6PPD-quinone | Sample source:Brain | Species:Chinook	Tank Replicate=B1; Experimental Replicate=2; RAW_FILE_NAME=142_6Q_B1_2_CH.mzML
SUBJECT_SAMPLE_FACTORS           	167	167_6Q_B1_1_RT	Treatment:6PPD-quinone | Sample source:Brain | Species:Rainbow Trout	Tank Replicate=B1; Experimental Replicate=1; RAW_FILE_NAME=167_6Q_B1_1_RT.mzML
SUBJECT_SAMPLE_FACTORS           	109	109_6Q_B1_1_CO	Treatment:6PPD-quinone | Sample source:Brain | Species:Coho	Tank Replicate=B1; Experimental Replicate=1; RAW_FILE_NAME=109_6Q_B1_1_CO.mzML
SUBJECT_SAMPLE_FACTORS           	126	126_6Q_B1_1_CH	Treatment:6PPD-quinone | Sample source:Brain | Species:Chinook	Tank Replicate=B1; Experimental Replicate=1; RAW_FILE_NAME=126_6Q_B1_1_CH.mzML
SUBJECT_SAMPLE_FACTORS           	212	212_IN_B5_1_CO	Treatment:Insult | Sample source:Brain | Species:Coho	Tank Replicate=B5; Experimental Replicate=1; RAW_FILE_NAME=212_IN_B5_1_CO.mzML
SUBJECT_SAMPLE_FACTORS           	213	213_CE_B5_2_CH	Treatment:Coexposure | Sample source:Brain | Species:Chinook	Tank Replicate=B5; Experimental Replicate=2; RAW_FILE_NAME=213_CE_B5_2_CH.mzML
SUBJECT_SAMPLE_FACTORS           	214	214_FC_B5_1_CO	Treatment:Fed Control | Sample source:Brain | Species:Coho	Tank Replicate=B5; Experimental Replicate=1; RAW_FILE_NAME=214_FC_B5_1_CO.mzML
SUBJECT_SAMPLE_FACTORS           	215	215_AQ_B4_2_RT	Treatment:Anthraquinone | Sample source:Brain | Species:Rainbow Trout	Tank Replicate=B4; Experimental Replicate=2; RAW_FILE_NAME=215_AQ_B4_2_RT.mzML
SUBJECT_SAMPLE_FACTORS           	216	216_AQ_B3_1_RT	Treatment:Anthraquinone | Sample source:Brain | Species:Rainbow Trout	Tank Replicate=B3; Experimental Replicate=1; RAW_FILE_NAME=216_AQ_B3_1_RT.mzML
SUBJECT_SAMPLE_FACTORS           	217	217_6Q_B3_2_RT	Treatment:6PPD-quinone | Sample source:Brain | Species:Rainbow Trout	Tank Replicate=B3; Experimental Replicate=2; RAW_FILE_NAME=217_6Q_B3_2_RT.mzML
SUBJECT_SAMPLE_FACTORS           	218	218_AQ_B3_2_CO	Treatment:Anthraquinone | Sample source:Brain | Species:Coho	Tank Replicate=B3; Experimental Replicate=2; RAW_FILE_NAME=218_AQ_B3_2_CO.mzML
SUBJECT_SAMPLE_FACTORS           	219	219_AQ_B3_1_CH	Treatment:Anthraquinone | Sample source:Brain | Species:Chinook	Tank Replicate=B3; Experimental Replicate=1; RAW_FILE_NAME=219_AQ_B3_1_CH.mzML
SUBJECT_SAMPLE_FACTORS           	221	221_AQ_B5_3_CO	Treatment:Anthraquinone | Sample source:Brain | Species:Coho	Tank Replicate=B5; Experimental Replicate=3; RAW_FILE_NAME=221_AQ_B5_3_CO.mzML
SUBJECT_SAMPLE_FACTORS           	210	210_AQ_B5_3_RT	Treatment:Anthraquinone | Sample source:Brain | Species:Rainbow Trout	Tank Replicate=B5; Experimental Replicate=3; RAW_FILE_NAME=210_AQ_B5_3_RT.mzML
#COLLECTION
CO:COLLECTION_SUMMARY            	Fish were sacrificed with MS222 and livers and brains were dissected, weighed,
CO:COLLECTION_SUMMARY            	and flash frozen in aluminum foil packets using liquid nitrogen and stored at
CO:COLLECTION_SUMMARY            	-80℃
CO:SAMPLE_TYPE                   	Brain, Liver
CO:STORAGE_CONDITIONS            	-80℃
#TREATMENT
TR:TREATMENT_SUMMARY             	Experimental exposures were conducted using a 2/3 static renewal system every
TR:TREATMENT_SUMMARY             	24-hours for a duration of 120-hours. Phase II experimental design included a
TR:TREATMENT_SUMMARY             	120-hour fasted control tank, a 120-hour fed control tank, and four chemical
TR:TREATMENT_SUMMARY             	treatment groups: a 120-hour 1 µg/L 6PPD-q exposure tank, a 120-hour 10 µg/L
TR:TREATMENT_SUMMARY             	AQ exposure tank, a 120-hour both 1 µg/L 6PPD-q and 10 µg/L AQ co-exposure
TR:TREATMENT_SUMMARY             	tank, and a 120-hour 10 µg/L AQ exposure tank combined with the 24-hour
TR:TREATMENT_SUMMARY             	exposure of 1 µg/L 6PPD-q. Each tank included five fish replicates and each
TR:TREATMENT_SUMMARY             	condition was repeated to collect three biological replicates (n = 3)
TR:TREATMENT_COMPOUND            	6PPD-quinone, Anthraquinone
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	Liver and brain tissues were allocated into 10 mg samples using a razor and dry
SP:SAMPLEPREP_SUMMARY            	ice. Samples were aliquoted in Eppendorf vials with 40 µL of 1.4 mm ceramic
SP:SAMPLEPREP_SUMMARY            	beads. Chilled methanol:water, 80:20, was added to the vials (300 µL). Samples
SP:SAMPLEPREP_SUMMARY            	were spiked with 3 µL of isotope labeled metabolite Mix 2 QReSS Kit (Cambridge
SP:SAMPLEPREP_SUMMARY            	Isotope Labs) to account for extraction variability among tissue samples.
SP:SAMPLEPREP_SUMMARY            	Samples were extracted in a Precellys homogenizer (3x15s; 5,500 rpm).
SP:SAMPLEPREP_SUMMARY            	Homogenized tissue (200 µL) was transferred to a clean Eppendorf vial with a
SP:SAMPLEPREP_SUMMARY            	corresponding randomly assigned ID number. Samples were centrifuged at 10,000g
SP:SAMPLEPREP_SUMMARY            	for 3 min at 4 °C. Samples were stored overnight at – 24 °C to precipitated
SP:SAMPLEPREP_SUMMARY            	proteins. Samples were centrifuged again at 13,000g for 15 min at 4 °C.
SP:SAMPLEPREP_SUMMARY            	Supernatant (150 µL) was recovered in a new Eppendorf tube and stored at 4 °C
SP:SAMPLEPREP_SUMMARY            	before LCMS analysis. Samples were spiked with 3 µL of isotope labeled
SP:SAMPLEPREP_SUMMARY            	metabolite Mix 1 QReSS Kit (Cambridge Isotope Labs) to account for instrumental
SP:SAMPLEPREP_SUMMARY            	variation between runs.
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_TYPE           	Reversed phase
CH:INSTRUMENT_NAME               	Sciex ExionLC AD
CH:COLUMN_NAME                   	GL Sciences Intersil Ph-3 (150 x 2.1 mm, 2µm)
CH:SOLVENT_A                     	100% Water; 0.1% Formic acid
CH:SOLVENT_B                     	100% Acetonitrile; 0.1% Formic acid
CH:FLOW_GRADIENT                 	In %B, at minute 0, 5% B, and held at 5% B until minute 3. From 3-12 minutes a
CH:FLOW_GRADIENT                 	gradient up to 98% B and held at 98% until 17 minutes. From 17 to 17.5 drop to
CH:FLOW_GRADIENT                 	5% B and held at 5% until 23 minutes.
CH:FLOW_RATE                     	0.3 mL/min
CH:COLUMN_TEMPERATURE            	40℃
CH:INJECTION_TEMPERATURE         	15
CH:INTERNAL_STANDARD             	QReSS Kit
CH:SAMPLE_INJECTION              	2 uL
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
#MS
MS:INSTRUMENT_NAME               	ABI Sciex 7600 ZenoTOF
MS:INSTRUMENT_TYPE               	QTOF
MS:MS_TYPE                       	ESI
MS:ION_MODE                      	NEGATIVE
MS:MS_COMMENTS                   	MSDIAL peak alignment with in-house IROA library annotations and MSDIAL public
MS:MS_COMMENTS                   	library.
MS:MS_RESULTS_FILE               	ST003294_AN005398_Results.txt	UNITS:abundance	Has m/z:Yes	Has RT:Yes	RT units:Minutes
#END