#METABOLOMICS WORKBENCH maloyan_20240819_120746 DATATRACK_ID:5121 STUDY_ID:ST003426 ANALYSIS_ID:AN005625 PROJECT_ID:PR002118
VERSION             	1
CREATED_ON             	August 22, 2024, 9:29 am
#PROJECT
PR:PROJECT_TITLE                 	Metabolomic and transcriptomic remodeling of bone marrow myeloid cells in
PR:PROJECT_TITLE                 	response to maternal obesity
PR:PROJECT_SUMMARY               	Maternal obesity puts the offspring at high risk of developing obesity and
PR:PROJECT_SUMMARY               	cardio-metabolic diseases in adulthood. Here, using a mouse model of maternal
PR:PROJECT_SUMMARY               	high-fat diet-induced obesity, we show that whole body fat content of the
PR:PROJECT_SUMMARY               	Off-HFD increases significantly from very early age when compared to the Off-RD.
PR:PROJECT_SUMMARY               	We have previously shown significant metabolic and immune perturbations in the
PR:PROJECT_SUMMARY               	bone marrow of newly weaned offspring of obese mothers. Therefore, we
PR:PROJECT_SUMMARY               	hypothesized that lipid metabolism is altered in the bone marrow Off-HFD in
PR:PROJECT_SUMMARY               	newly weaned offspring of obese mothers when compared to the Off-RD. To test
PR:PROJECT_SUMMARY               	this hypothesis, we investigated the lipidomic profile of bone marrow cells
PR:PROJECT_SUMMARY               	collected from three-week-old offspring of regular and high fat diet-fed
PR:PROJECT_SUMMARY               	mothers. Diacylgycerols (DAGs), triacylglycerols (TAGs), sphingolipids and
PR:PROJECT_SUMMARY               	phospholipids, including plasmalogen, and lysophospholipids were remarkably
PR:PROJECT_SUMMARY               	different between the groups, independent of fetal sex. Levels of cholesteryl
PR:PROJECT_SUMMARY               	esters were significantly decreased in offspring of obese mothers, suggesting
PR:PROJECT_SUMMARY               	reduced delivery of cholesterol to bone marrow cells. This was accompanied by
PR:PROJECT_SUMMARY               	age-dependent progression of mitochondrial dysfunction in bone marrow cells. We
PR:PROJECT_SUMMARY               	subsequently isolated CD11b+ myeloid cells from three-week-old mice and
PR:PROJECT_SUMMARY               	conducted metabolomics, lipidomics, and transcriptomics analyses. The lipidomic
PR:PROJECT_SUMMARY               	profiles of these bone marrow myeloid cells were largely similar to that seen in
PR:PROJECT_SUMMARY               	bone marrow cells and included increases in DAGs and phospholipids alongside
PR:PROJECT_SUMMARY               	decreased TAGs, except for long-chain TAGs, which were significantly increased.
PR:PROJECT_SUMMARY               	Our data also revealed significant sex-dependent changes in amino acids and
PR:PROJECT_SUMMARY               	metabolites related to energy metabolism. Transcriptomic analysis revealed
PR:PROJECT_SUMMARY               	altered expression of genes related to major immune pathways including
PR:PROJECT_SUMMARY               	macrophage alternative activation, B-cell receptor signaling, TGF signaling,
PR:PROJECT_SUMMARY               	and communication between the innate and adaptive immune systems. All told, this
PR:PROJECT_SUMMARY               	study revealed lipidomic, metabolomic, and gene expression abnormalities in bone
PR:PROJECT_SUMMARY               	marrow cells broadly, and in bone marrow myeloid cells particularly, in the
PR:PROJECT_SUMMARY               	newly-weaned offspring of obese mothers, which might at least partially explain
PR:PROJECT_SUMMARY               	the progression of metabolic and cardiovascular diseases in their adulthood.
PR:INSTITUTE                     	Oregon Health and Science University
PR:DEPARTMENT                    	Knight Cardiovascular Institute
PR:LABORATORY                    	Maloyan Laboratory
PR:LAST_NAME                     	Maloyan
PR:FIRST_NAME                    	Alina
PR:ADDRESS                       	3181 S.W. Sam Jackson Park Road, Portland, Oregon, 97239-3098, USA
PR:EMAIL                         	maloyan@ohsu.edu
PR:PHONE                         	513-238-3783
#STUDY
ST:STUDY_TITLE                   	Metabolomic and transcriptomic remodeling of bone marrow myeloid cells in
ST:STUDY_TITLE                   	response to maternal obesity
ST:STUDY_SUMMARY                 	Maternal obesity puts the offspring at high risk of developing obesity and
ST:STUDY_SUMMARY                 	cardio-metabolic diseases in adulthood. Here, using a mouse model of maternal
ST:STUDY_SUMMARY                 	high-fat diet-induced obesity, we show that whole body fat content of the
ST:STUDY_SUMMARY                 	Off-HFD increases significantly from very early age when compared to the Off-RD.
ST:STUDY_SUMMARY                 	We have previously shown significant metabolic and immune perturbations in the
ST:STUDY_SUMMARY                 	bone marrow of newly weaned offspring of obese mothers. Therefore, we
ST:STUDY_SUMMARY                 	hypothesized that lipid metabolism is altered in the bone marrow Off-HFD in
ST:STUDY_SUMMARY                 	newly weaned offspring of obese mothers when compared to the Off-RD. To test
ST:STUDY_SUMMARY                 	this hypothesis, we investigated the lipidomic profile of bone marrow cells
ST:STUDY_SUMMARY                 	collected from three-week-old offspring of regular and high fat diet-fed
ST:STUDY_SUMMARY                 	mothers. Diacylgycerols (DAGs), triacylglycerols (TAGs), sphingolipids and
ST:STUDY_SUMMARY                 	phospholipids, including plasmalogen, and lysophospholipids were remarkably
ST:STUDY_SUMMARY                 	different between the groups, independent of fetal sex. Levels of cholesteryl
ST:STUDY_SUMMARY                 	esters were significantly decreased in offspring of obese mothers, suggesting
ST:STUDY_SUMMARY                 	reduced delivery of cholesterol to bone marrow cells. This was accompanied by
ST:STUDY_SUMMARY                 	age-dependent progression of mitochondrial dysfunction in bone marrow cells. We
ST:STUDY_SUMMARY                 	subsequently isolated CD11b+ myeloid cells from three-week-old mice and
ST:STUDY_SUMMARY                 	conducted metabolomics, lipidomics, and transcriptomics analyses. The lipidomic
ST:STUDY_SUMMARY                 	profiles of these bone marrow myeloid cells were largely similar to that seen in
ST:STUDY_SUMMARY                 	bone marrow cells and included increases in DAGs and phospholipids alongside
ST:STUDY_SUMMARY                 	decreased TAGs, except for long-chain TAGs, which were significantly increased.
ST:STUDY_SUMMARY                 	Our data also revealed significant sex-dependent changes in amino acids and
ST:STUDY_SUMMARY                 	metabolites related to energy metabolism. Transcriptomic analysis revealed
ST:STUDY_SUMMARY                 	altered expression of genes related to major immune pathways including
ST:STUDY_SUMMARY                 	macrophage alternative activation, B-cell receptor signaling, TGF signaling,
ST:STUDY_SUMMARY                 	and communication between the innate and adaptive immune systems. All told, this
ST:STUDY_SUMMARY                 	study revealed lipidomic, metabolomic, and gene expression abnormalities in bone
ST:STUDY_SUMMARY                 	marrow cells broadly, and in bone marrow myeloid cells particularly, in the
ST:STUDY_SUMMARY                 	newly-weaned offspring of obese mothers, which might at least partially explain
ST:STUDY_SUMMARY                 	the progression of metabolic and cardiovascular diseases in their adulthood.
ST:INSTITUTE                     	Oregon Health and Science University
ST:LABORATORY                    	Maloyan Laboratory
ST:LAST_NAME                     	Maloyan
ST:FIRST_NAME                    	Alina
ST:ADDRESS                       	3181 S.W. Sam Jackson Park Road, Portland, Oregon, 97239-3098, USA
ST:EMAIL                         	maloyan@ohsu.edu
ST:PHONE                         	513-238-3783
#SUBJECT
SU:SUBJECT_TYPE                  	Mammal
SU:SUBJECT_SPECIES               	Mus musculus
SU:TAXONOMY_ID                   	10090
SU:AGE_OR_AGE_RANGE              	3 week old
SU:GENDER                        	Male and female
SU:ANIMAL_HOUSING                	regular
SU:ANIMAL_FEED                   	Fed by a regular or high fat diet
SU:ANIMAL_WATER                  	ad libitum
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data
SUBJECT_SAMPLE_FACTORS           	-	Maloyan_Asth_FOH2_l_20	Offspring group:Offspring of High-Fat-Diet | Sex:Female | Sample source:Bone marrow	RAW_FILE_NAME=Maloyan_Asth_FOH2_l_20
SUBJECT_SAMPLE_FACTORS           	-	Maloyan_Asth_FOH3_l_21	Offspring group:Offspring of High-Fat-Diet | Sex:Female | Sample source:Bone marrow	RAW_FILE_NAME=Maloyan_Asth_FOH3_l_21
SUBJECT_SAMPLE_FACTORS           	-	Maloyan_Asth_FOH4_l_22	Offspring group:Offspring of High-Fat-Diet | Sex:Female | Sample source:Bone marrow	RAW_FILE_NAME=Maloyan_Asth_FOH4_l_22
SUBJECT_SAMPLE_FACTORS           	-	Maloyan_Asth_FOH5_l_23	Offspring group:Offspring of High-Fat-Diet | Sex:Female | Sample source:Bone marrow	RAW_FILE_NAME=Maloyan_Asth_FOH5_l_23
SUBJECT_SAMPLE_FACTORS           	-	Maloyan_Asth_FOH6_l_24	Offspring group:Offspring of High-Fat-Diet | Sex:Female | Sample source:Bone marrow	RAW_FILE_NAME=Maloyan_Asth_FOH6_l_24
SUBJECT_SAMPLE_FACTORS           	-	Maloyan_Asth_FOR1_l_13	Offspring group:Offspring of Regular Diet | Sex:Female | Sample source:Bone marrow	RAW_FILE_NAME=Maloyan_Asth_FOR1_l_13
SUBJECT_SAMPLE_FACTORS           	-	Maloyan_Asth_FOR2_l_14	Offspring group:Offspring of Regular Diet | Sex:Female | Sample source:Bone marrow	RAW_FILE_NAME=Maloyan_Asth_FOR2_l_14
SUBJECT_SAMPLE_FACTORS           	-	Maloyan_Asth_FOR3_l_15	Offspring group:Offspring of Regular Diet | Sex:Female | Sample source:Bone marrow	RAW_FILE_NAME=Maloyan_Asth_FOR3_l_15
SUBJECT_SAMPLE_FACTORS           	-	Maloyan_Asth_FOR4_l_16	Offspring group:Offspring of Regular Diet | Sex:Female | Sample source:Bone marrow	RAW_FILE_NAME=Maloyan_Asth_FOR4_l_16
SUBJECT_SAMPLE_FACTORS           	-	Maloyan_Asth_FOR5_l_17	Offspring group:Offspring of Regular Diet | Sex:Female | Sample source:Bone marrow	RAW_FILE_NAME=Maloyan_Asth_FOR5_l_17
SUBJECT_SAMPLE_FACTORS           	-	Maloyan_Asth_FOR6_l_18	Offspring group:Offspring of Regular Diet | Sex:Female | Sample source:Bone marrow	RAW_FILE_NAME=Maloyan_Asth_FOR6_l_18
SUBJECT_SAMPLE_FACTORS           	-	Maloyan_Asth_MOH1_l_07	Offspring group:Offspring of High-Fat-Diet | Sex:Male | Sample source:Bone marrow	RAW_FILE_NAME=Maloyan_Asth_MOH1_l_07
SUBJECT_SAMPLE_FACTORS           	-	Maloyan_Asth_MOH2_l_08	Offspring group:Offspring of High-Fat-Diet | Sex:Male | Sample source:Bone marrow	RAW_FILE_NAME=Maloyan_Asth_MOH2_l_08
SUBJECT_SAMPLE_FACTORS           	-	Maloyan_Asth_MOH3_l_09	Offspring group:Offspring of High-Fat-Diet | Sex:Male | Sample source:Bone marrow	RAW_FILE_NAME=Maloyan_Asth_MOH3_l_09
SUBJECT_SAMPLE_FACTORS           	-	Maloyan_Asth_MOH4_l_10	Offspring group:Offspring of High-Fat-Diet | Sex:Male | Sample source:Bone marrow	RAW_FILE_NAME=Maloyan_Asth_MOH4_l_10
SUBJECT_SAMPLE_FACTORS           	-	Maloyan_Asth_MOH5_l_11	Offspring group:Offspring of High-Fat-Diet | Sex:Male | Sample source:Bone marrow	RAW_FILE_NAME=Maloyan_Asth_MOH5_l_11
SUBJECT_SAMPLE_FACTORS           	-	Maloyan_Asth_MOH6_l_12	Offspring group:Offspring of High-Fat-Diet | Sex:Male | Sample source:Bone marrow	RAW_FILE_NAME=Maloyan_Asth_MOH6_l_12
SUBJECT_SAMPLE_FACTORS           	-	Maloyan_Asth_MOR1_l_01	Offspring group:Offspring of Regular Diet | Sex:Male | Sample source:Bone marrow	RAW_FILE_NAME=Maloyan_Asth_MOR1_l_01
SUBJECT_SAMPLE_FACTORS           	-	Maloyan_Asth_MOR2_l_02	Offspring group:Offspring of Regular Diet | Sex:Male | Sample source:Bone marrow	RAW_FILE_NAME=Maloyan_Asth_MOR2_l_02
SUBJECT_SAMPLE_FACTORS           	-	Maloyan_Asth_MOR3_l_03	Offspring group:Offspring of Regular Diet | Sex:Male | Sample source:Bone marrow	RAW_FILE_NAME=Maloyan_Asth_MOR3_l_03
SUBJECT_SAMPLE_FACTORS           	-	Maloyan_Asth_MOR4_l_04	Offspring group:Offspring of Regular Diet | Sex:Male | Sample source:Bone marrow	RAW_FILE_NAME=Maloyan_Asth_MOR4_l_04
SUBJECT_SAMPLE_FACTORS           	-	Maloyan_Asth_MOR5_l_05	Offspring group:Offspring of Regular Diet | Sex:Male | Sample source:Bone marrow	RAW_FILE_NAME=Maloyan_Asth_MOR5_l_05
SUBJECT_SAMPLE_FACTORS           	-	Maloyan_Asth_MOR6_l_06	Offspring group:Offspring of Regular Diet | Sex:Male | Sample source:Bone marrow	RAW_FILE_NAME=Maloyan_Asth_MOR6_l_06
SUBJECT_SAMPLE_FACTORS           	1	Maloyan_Asth_FOH1_l_19	Offspring group:Offspring of High-Fat-Diet | Sex:Female | Sample source:Bone marrow	RAW_FILE_NAME=Maloyan_Asth_FOH1_l_19
#COLLECTION
CO:COLLECTION_SUMMARY            	Bone marrow cells were isolated as previously reported (20). Briefly, femurs
CO:COLLECTION_SUMMARY            	were flushed using a 25G5/8 needle with medium containing RPMI1640 + 2% FBS + 10
CO:COLLECTION_SUMMARY            	units/ml heparin + penicillin and streptomycin. Bits of bone were removed using
CO:COLLECTION_SUMMARY            	a sterile 4 mm nylon cell strainer (Falcon 352340). Cells were dissolved in 50
CO:COLLECTION_SUMMARY            	ml medium and centrifuged at 2000 rpm (900 x g) for ten minutes at 4 °C. The
CO:COLLECTION_SUMMARY            	resultant cell pellets were washed twice with 50 ml of serum-free RPMI (RPMI1640
CO:COLLECTION_SUMMARY            	+ 20 mM Hepes + penicillin and streptomycin, adjusted to pH 7.4 before
CO:COLLECTION_SUMMARY            	filtering), centrifuged at 2000 rpm at 4 °C for five minutes, and finally
CO:COLLECTION_SUMMARY            	resuspended in 25 ml of serum-free RPMI. The obtained bone marrow cells were
CO:COLLECTION_SUMMARY            	enriched for cells expressing CD11b using anti-CD11b magnetic beads (STEM CELL
CO:COLLECTION_SUMMARY            	Technologies, Vancouver, BC), applied according to the manufacturer’s
CO:COLLECTION_SUMMARY            	instructions. Isolated CD11b+ cells were sent for metabolomic, lipidomic, and
CO:COLLECTION_SUMMARY            	transcriptomic analyses.
CO:COLLECTION_PROTOCOL_FILENAME  	Lipidomics_and_metabolomics.pdf
CO:SAMPLE_TYPE                   	Bone marrow
#TREATMENT
TR:TREATMENT_SUMMARY             	To induce maternal obesity, high-fat diet (Teklad Cat#TD.06415) or its control,
TR:TREATMENT_SUMMARY             	a regular diet (RD, PicoLab® Laboratory Rodent Diet Cat # 5LOD) was given to
TR:TREATMENT_SUMMARY             	virgin female FVB/NJ mice from the six-weeks of age and throughout the entire
TR:TREATMENT_SUMMARY             	study. After eight weeks of dietary intervention, RD- and HFD-fed female mice
TR:TREATMENT_SUMMARY             	were bred to an age-matched RD-fed male. At weaning, male and female offspring
TR:TREATMENT_SUMMARY             	from each mother were randomly selected for the study. The offspring were fed a
TR:TREATMENT_SUMMARY             	regular diet only starting from weaning and throughout their life.
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	Lipids and metabolites were extracted from cells using MPLEx (Metabolite,
SP:SAMPLEPREP_SUMMARY            	Protein, Lipid Extraction). First, 1 mL cold (-20 °C) chloroform:methanol
SP:SAMPLEPREP_SUMMARY            	working mix (prepared 2:1, v/v) was added into a chloroform-compatible 2 mL
SP:SAMPLEPREP_SUMMARY            	Sorenson MulTI™ SafeSeal™ microcentrifuge tube (Sorenson Bioscience, Salt
SP:SAMPLEPREP_SUMMARY            	Lake City, UT) inside an ice-block. The sample and cold water were then added to
SP:SAMPLEPREP_SUMMARY            	make a final ratio of 8:4:3 chloroform:methanol:water/sample, vortexed, allowed
SP:SAMPLEPREP_SUMMARY            	to incubate in the ice block for 15 mins with 2000 rpm shaking, and finally
SP:SAMPLEPREP_SUMMARY            	sonicated in an ice water bath sonicator. Afterwards, the samples were
SP:SAMPLEPREP_SUMMARY            	centrifuged at 12,000 x g for 10 mins to separate the polar and non-polar phases
SP:SAMPLEPREP_SUMMARY            	and the protein interlayer. The upper polar phase and a portion of the lower
SP:SAMPLEPREP_SUMMARY            	non-polar phase were allotted to metabolite samples, with the remaining lower
SP:SAMPLEPREP_SUMMARY            	non-polar phase used for lipid samples. The whole process was repeated once on
SP:SAMPLEPREP_SUMMARY            	the protein interlayer to ensure complete removal of metabolites and lipids. The
SP:SAMPLEPREP_SUMMARY            	protein was then washed with 500 µl of cold 100% methanol and centrifuged for
SP:SAMPLEPREP_SUMMARY            	five minutes to pellet the protein, and the wash layer was added to the
SP:SAMPLEPREP_SUMMARY            	metabolites vial. Metabolite and lipid samples were dried in a speed vac, and
SP:SAMPLEPREP_SUMMARY            	500 µl 2:1 (v:v) cold chloroform:methanol was added to the lipids, which were
SP:SAMPLEPREP_SUMMARY            	ultimately stored along with the dry metabolites at -20 °C until analysis.
SP:SAMPLEPREP_PROTOCOL_FILENAME  	Lipidomics_and_metabolomics.pdf
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_TYPE           	Other
CH:INSTRUMENT_NAME               	Waters Acquity H-Class
CH:COLUMN_NAME                   	Waters ACQUITY UPLC CSH C18 (150 x 3mm, 1.7um)
CH:SOLVENT_A                     	40% acetonitrile/60% water; 10 mM ammonium acetate
CH:SOLVENT_B                     	10% acetonitrile/90% isopropanol; 10 mM ammonium acetate
CH:FLOW_GRADIENT                 	Time (min), %B: 0, 40; 2, 50; 3, 60; 12, 70; 15, 75; 17, 78; 19, 85; 22, 92; 25,
CH:FLOW_GRADIENT                 	99; 34, 99; and 34.5, 40
CH:FLOW_RATE                     	250 uL/min
CH:COLUMN_TEMPERATURE            	42
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
AN:ANALYSIS_PROTOCOL_FILE        	Lipidomics_and_metabolomics.pdf
#MS
MS:INSTRUMENT_NAME               	Thermo Velos Pro Orbitrap
MS:INSTRUMENT_TYPE               	Orbitrap
MS:MS_TYPE                       	ESI
MS:ION_MODE                      	POSITIVE
MS:MS_COMMENTS                   	Lipid identifications were made using MS-DIAL v4.92 for peak detection,
MS:MS_COMMENTS                   	identification, and alignment. The tandem mass spectra and corresponding
MS:MS_COMMENTS                   	fragment ions, mass measurement error, and aligned chromatographic features were
MS:MS_COMMENTS                   	manually examined to remove false positives. Relative quantification was
MS:MS_COMMENTS                   	performed by calculating peak areas on extracted ion chromatograms of precursor
MS:MS_COMMENTS                   	masses
#MS_METABOLITE_DATA
MS_METABOLITE_DATA:UNITS	median normalized log 2 transformed peak areas
MS_METABOLITE_DATA_START
Samples	Maloyan_Asth_FOH1_l_19	Maloyan_Asth_FOH2_l_20	Maloyan_Asth_FOH3_l_21	Maloyan_Asth_FOH4_l_22	Maloyan_Asth_FOH5_l_23	Maloyan_Asth_FOH6_l_24	Maloyan_Asth_FOR1_l_13	Maloyan_Asth_FOR2_l_14	Maloyan_Asth_FOR3_l_15	Maloyan_Asth_FOR4_l_16	Maloyan_Asth_FOR5_l_17	Maloyan_Asth_FOR6_l_18	Maloyan_Asth_MOH1_l_07	Maloyan_Asth_MOH2_l_08	Maloyan_Asth_MOH3_l_09	Maloyan_Asth_MOH4_l_10	Maloyan_Asth_MOH5_l_11	Maloyan_Asth_MOH6_l_12	Maloyan_Asth_MOR1_l_01	Maloyan_Asth_MOR2_l_02	Maloyan_Asth_MOR3_l_03	Maloyan_Asth_MOR4_l_04	Maloyan_Asth_MOR5_l_05	Maloyan_Asth_MOR6_l_06	
Factors	Offspring group:Offspring of High-Fat-Diet | Sex:Female | Sample source:Bone marrow	Offspring group:Offspring of High-Fat-Diet | Sex:Female | Sample source:Bone marrow	Offspring group:Offspring of High-Fat-Diet | Sex:Female | Sample source:Bone marrow	Offspring group:Offspring of High-Fat-Diet | Sex:Female | Sample source:Bone marrow	Offspring group:Offspring of High-Fat-Diet | Sex:Female | Sample source:Bone marrow	Offspring group:Offspring of High-Fat-Diet | Sex:Female | Sample source:Bone marrow	Offspring group:Offspring of Regular Diet | Sex:Female | Sample source:Bone marrow	Offspring group:Offspring of Regular Diet | Sex:Female | Sample source:Bone marrow	Offspring group:Offspring of Regular Diet | Sex:Female | Sample source:Bone marrow	Offspring group:Offspring of Regular Diet | Sex:Female | Sample source:Bone marrow	Offspring group:Offspring of Regular Diet | Sex:Female | Sample source:Bone marrow	Offspring group:Offspring of Regular Diet | Sex:Female | Sample source:Bone marrow	Offspring group:Offspring of High-Fat-Diet | Sex:Male | Sample source:Bone marrow	Offspring group:Offspring of High-Fat-Diet | Sex:Male | Sample source:Bone marrow	Offspring group:Offspring of High-Fat-Diet | Sex:Male | Sample source:Bone marrow	Offspring group:Offspring of High-Fat-Diet | Sex:Male | Sample source:Bone marrow	Offspring group:Offspring of High-Fat-Diet | Sex:Male | Sample source:Bone marrow	Offspring group:Offspring of High-Fat-Diet | Sex:Male | Sample source:Bone marrow	Offspring group:Offspring of Regular Diet | Sex:Male | Sample source:Bone marrow	Offspring group:Offspring of Regular Diet | Sex:Male | Sample source:Bone marrow	Offspring group:Offspring of Regular Diet | Sex:Male | Sample source:Bone marrow	Offspring group:Offspring of Regular Diet | Sex:Male | Sample source:Bone marrow	Offspring group:Offspring of Regular Diet | Sex:Male | Sample source:Bone marrow	Offspring group:Offspring of Regular Diet | Sex:Male | Sample source:Bone marrow
Adenosine 5'-monophosphate	17.37005312	18.1338288	17.54990544	20.36326403	20.91873026	19.10358481	17.17402983	19.05717769	19.06276569	19.0888383	18.60539116	18.05055055	12.88873485	18.46932613	17.55920423	20.04398772	18.83035938	18.54052322	18.07085559	13.16924343	16.70317665	16.00618215	16.5569452	18.03548225	
CAR 16:0	24.15707041	24.11817581	23.52885025	23.86687454	23.49817141	23.46027625	23.85867933	24.10212168	23.67957453	23.86966194	24.40565141	24.5996388	24.54065485	23.56965602	24.23418885	23.98968599	24.01972171	23.53179985	24.05594264	22.71220098	23.39687218	23.16064059	23.90298041	24.23113267	
CAR 18:0	21.36113607	21.05709276	20.37138594	20.24218363	20.56429258	20.64871211	20.36277206	20.85889973	20.85701219	20.39000225	21.07887588	21.04215252	20.73989458	20.42817636	20.69215666	20.82427858	20.77675739	20.67381254	20.33996823	20.01448301	19.69082617	19.58195277	20.54868581	20.77793536	
CAR 18:1	22.73199898	22.10240158	21.14626703	21.51086682	21.51802359	21.41033565	21.84496605	22.13791992	22.09746234	21.66005479	22.19664866	22.43220971	22.33414163	21.50325107	22.29171081	22.39503975	22.34234787	22.14737696	22.04243474	21.15367433	21.19917403	21.01091375	22.15207222	22.28614593	
CE 18:1	22.1635292	22.13296269	23.99878317	22.91953423	23.22833545	22.55339762	23.05759244	22.84485406	23.29576487	22.60661307	21.99042056	22.65687089	21.53772442	21.29590884	21.48140772	21.47120908	21.72512587	21.58575862	21.26382986	21.08036975	21.43862375	21.41533893	21.46879999	21.72244004	
CE 18:2	21.97578676	21.97325353	24.29394119	22.94438593	23.59819733	22.56020995	23.10590462	22.9781159	23.47245243	22.63969019	21.99165856	22.74278671	21.01867063	20.89961297	21.30777528	21.22551109	21.3838528	21.45889992	21.20633692	20.80661564	21.18632001	21.21907983	21.23210731	21.52149551	
CE 20:3	20.46492236	20.28346205	22.08497109	20.77452828	21.36944403	20.57577338	20.88469699	20.97868806	21.29068868	20.55378883	20.17069143	20.66881582	19.81316423	19.45034338	19.86157146	20.01743301	20.04242555	20.04032156	19.84877699	19.38953239	19.84907471	19.74861825	19.82589745	20.13902562	
CE 20:4	21.55605333	21.37403584	24.12754799	22.57879474	23.37941314	22.11462657	22.80543897	22.59094071	23.08688847	22.22283351	21.48711576	22.46086185	20.54100988	20.39216693	20.83845412	20.78427184	20.88474934	21.00621308	20.96877558	20.38700446	20.60520744	20.66803631	20.42929347	20.80367942	
CE 20:5	18.86232475	18.93201864	21.89787924	20.08306191	21.08348165	19.42600586	20.35771341	20.18703241	20.81657365	19.65347471	18.9695656	20.01395789	17.99150565	17.75693751	18.0534708	18.12171579	18.17686262	18.17845065	18.18760007	17.78194766	17.93930259	18.27034752	18.27228059	18.37236789	
CE 22:6_LipPos	20.60268297	20.45536833	22.63267747	20.98882331	22.01573788	20.69199635	21.3478792	21.41619334	21.69803839	20.86182716	20.45754888	21.07176038	20.11298939	19.8368874	20.39514579	20.40055192	20.42762228	20.25359085	20.06458166	19.55764337	20.12874333	19.98061607	20.35142851	20.66258096	
Cer 33:3;2O|Cer 18:1;2O/15:2	17.18197577	16.07096792	17.45824943	18.43203189	16.45361901	17.14611091	17.43087846	17.16230252	16.67116352	17.9383217	16.65924659	17.7161247	18.27466105	17.68219286	16.52538092	16.96757341	16.75378866	17.09134196	17.25203261	18.09471508	18.35061856	18.84195194	18.05122933	17.57185944	
Cer 34:1;2O|Cer 18:1;2O/16:0	21.17235567	21.1636876	20.95010294	21.26434224	20.93364557	21.19653247	21.22107122	21.30267929	21.35953596	21.32040204	21.23425378	21.29387367	21.7262368	21.31129883	21.46010351	21.54385917	21.38402599	21.42676745	20.90985121	20.49784525	21.05495789	21.21647202	21.41133516	21.53259161	
Cer 40:1;2O|Cer 18:1;2O/22:0	19.99243267	19.87241002	19.57353278	19.76099718	19.75879062	19.6561512	19.85495371	20.09202266	20.0923795	19.97740979	19.94534034	20.16599853	20.26257532	19.68177967	20.14990627	20.18692664	20.05925107	20.12453744	19.50929644	18.67498152	19.73684161	19.48272144	20.05213041	20.27135598	
Cer 42:1;2O|Cer 18:1;2O/24:0_A	18.49137922	18.53886722	18.08662699	18.08370167	18.30636655	18.31618674	18.61543434	18.78143743	18.61240493	18.62691804	18.62171467	18.86525612	18.79896535	17.96589445	18.84929385	18.77867629	18.81145124	18.74834427	18.29895896	17.27973479	18.13936206	17.6660338	18.60135823	18.87638198	
Cer 42:1;2O|Cer 18:1;2O/24:0_B	20.47226455	20.33128561	20.09117022	20.01367557	20.28261268	20.24168918	20.28398367	20.6107689	20.4831623	20.20907017	20.43673862	20.47420694	20.46454583	20.11934938	20.67300124	20.7307091	20.54217693	20.61780474	20.16748627	19.21687957	19.89875714	19.85057392	20.42395206	20.68987927	
Cer 42:2;2O|Cer 18:1;2O/24:1	21.85651988	21.70666207	21.51239943	21.59838339	21.54000824	21.5952194	21.58118898	21.83064528	21.83608508	21.67387291	21.72809658	21.86111394	22.10115859	21.63357691	22.05585211	22.11815595	22.05305687	22.02473693	21.45884628	20.50814491	21.27946506	20.98368002	21.72652032	21.97503262	
Cer 42:3;2O|Cer 18:1;2O/24:2	19.936992	19.85334747	19.67272722	19.69552292	19.66385948	19.82981585	19.90390922	20.27758328	20.10139255	20.03989765	19.9448604	19.88652099	20.22928792	19.59775097	20.13646584	20.14057814	20.10962994	20.21572394	19.74013668	18.81719407	19.62863965	19.57095591	19.99198628	20.2981945	
DG 32:0|DG 16:0_16:0	21.79222148	21.71511032	21.57762246	21.90588579	21.86635303	21.9386489	21.91507366	21.90483084	21.83485795	21.74087124	21.93015124	22.00678196	22.27028687	21.87392158	22.24253534	22.22627528	22.01025492	22.17496454	22.12029162	21.74240336	22.08153076	22.1900139	22.15630884	22.03059148	
DG 34:0|DG 16:0_18:0	21.28072591	21.17831563	21.25780322	21.56553998	21.28105625	21.35274192	21.38516004	21.39238932	21.33362243	21.46054626	21.41405891	21.52022883	21.98886638	21.73265578	21.51957842	21.53783806	21.38488536	21.53844555	21.84651721	21.67773794	21.81143192	22.10190942	21.72049343	21.78577391	
DG 34:1|DG 16:0_18:1	21.98872021	21.99011353	21.84754028	21.87738233	21.970893	21.99543366	21.70693842	21.7158392	21.70616828	21.61405668	21.88483517	21.85700737	22.16835814	21.86301198	22.35609704	22.43396514	22.10233357	22.19549526	21.70674429	21.21919538	21.82709909	21.84058443	22.00668546	22.04304653	
DG 34:2|DG 16:0_18:2	21.21909728	21.37215496	21.1780277	21.36886149	21.35828412	21.32576609	21.38271874	21.34358527	21.34498183	21.28494606	21.32839362	21.37980165	21.70448027	21.38361812	21.86193495	21.80849539	21.51145706	21.58328771	21.34037573	20.66456813	21.44411328	21.42974623	21.61228217	21.72481833	
DG 35:2|DG 20:0_15:2	15.27343659	15.72874915	15.73235305	15.15440015	15.64341405	15.93116181	15.7814783	15.38100612	16.08710561	16.15167837	15.15850699	15.42407424	16.41482233	15.94901295	15.66400132	16.3530232	15.37853585	15.46310438	15.67141443	16.08619287	16.60104728	16.52966585	16.25262608	16.15344775	
DG 36:0|DG 18:0_18:0	19.61751038	19.75988974	19.83475562	20.57260562	19.62923741	19.8860624	20.2228968	20.1143873	20.07808789	20.61847815	20.10165756	20.67261377	21.14759138	20.9925855	19.89158779	19.84896493	20.05338359	19.86301411	21.09937026	21.08369965	21.0310424	21.44485796	20.68924414	20.91161468	
DG 36:1|DG 18:0_18:1	21.06684912	21.0147677	20.8121581	20.79643061	21.0271005	21.04123478	20.62731693	20.72577422	20.61512184	20.64230872	20.90243287	20.86381125	21.18372693	20.68219133	21.30332119	21.2966697	21.0422314	21.2971119	20.809932	19.98808008	20.6336443	20.62125824	20.9764513	20.9314385	
DG 36:2|DG 18:0_18:2	22.09688302	22.12388127	21.91980355	22.04289579	22.04689964	22.10845991	21.86948602	21.95847187	21.91539224	21.90864878	21.92655567	21.94134798	22.33132148	22.0694359	22.39543232	22.45217653	22.14256737	22.21532019	21.91364069	21.22304079	21.87769963	21.93090175	22.15831809	22.23064456	
DG 36:3|DG 18:1_18:2	21.61356682	21.57202551	21.16331457	21.58890232	21.58342503	21.80182057	21.54073681	21.61669166	21.61905538	21.5011752	21.37902378	21.59551604	21.80934467	21.41149266	21.94472736	22.03921392	21.54080731	21.44523734	21.18302685	20.59177404	21.28745359	21.43016436	21.65466418	21.6992923	
DG 38:4|DG 18:0_20:4	21.40674272	21.46672775	21.21139455	21.24701026	21.42184138	21.44310424	21.14392972	21.37548946	21.36937745	21.35710794	21.21165122	21.31572381	21.12680795	21.45114817	21.90521075	21.95946618	21.6779954	21.69173976	21.29944423	20.48861158	21.22595997	21.35253986	21.18788189	21.40882378	
LPC 15:0/0:0	21.45193603	21.75861688	21.28052872	20.96810408	21.63482793	21.58685767	21.35998852	21.52922786	21.44694375	21.45666809	21.62450226	21.46652389	21.28821669	21.38426066	22.07803093	22.11926081	21.99411147	21.84144347	21.37649134	19.94820281	20.99523286	20.95571925	21.18130697	21.61665282	
LPC 18:0/0:0	23.22724706	23.58791349	23.63820107	23.22513353	23.81727088	23.32244378	23.45744269	23.82929376	23.49728276	24.04864266	23.39333377	23.88526473	23.51389995	23.0175734	24.33760017	23.58670405	23.62808484	23.4396679	23.80749065	22.3125437	23.31281317	23.52727768	23.23491658	23.46623867	
LPC 18:1	22.90260265	22.71187164	23.03097624	22.21812107	22.83718174	22.70272292	22.41612143	22.44457544	22.7578106	22.54378458	22.77393365	22.67425467	22.32242648	22.23549427	23.25729256	23.20169167	22.98579399	23.03980498	22.55307301	20.26940984	21.86546107	21.20725775	22.1270528	22.64071788	
LPC 18:2	22.76508366	22.73042631	22.80002029	22.34141229	22.6184241	22.72374709	22.65702344	22.64071563	22.84136738	22.76175472	23.03915063	22.7296614	22.51432288	22.31833668	23.25779507	23.08103926	22.90727746	22.78212634	22.73471185	20.86115841	21.95321168	21.85528504	22.43050404	22.79450035	
LPC 19:0	17.17463924	17.53030608	17.32311704	16.97044237	17.76623681	17.32351345	17.04926867	17.46524361	17.76294024	17.41770295	17.29589351	17.3329468	17.21783671	16.77527778	17.49605007	17.32409501	17.26004418	17.64721316	17.23178661	16.29773189	16.58123063	16.79255714	16.54584847	17.28198599	
LPC 20:4	22.30532708	22.25723138	22.48386615	21.81197577	22.26344038	22.13876913	21.90963251	21.99583637	22.24240322	22.21298813	22.43291569	22.1898895	21.63654001	21.77816045	22.72893389	22.72301728	22.51462291	22.3039021	21.95311915	20.06941797	21.33266766	21.24309745	21.35654362	21.81956667	
LPC 22:6	19.35037617	19.52810672	20.99150722	19.27153255	20.48489139	19.50251209	19.8021882	19.68272156	20.32028852	19.69443217	19.69944648	19.78056691	18.67310372	19.02929534	20.17068619	20.0516986	19.86524524	19.66556742	19.44045331	17.1719286	18.90391133	18.7181626	18.79071721	19.05992203	
MG 18:0_A	16.55284175	16.37493975	17.11927109	17.47381886	16.38069589	17.19213687	17.06578311	16.64034466	16.9025122	17.17107878	16.77494222	17.1427955	18.14005433	17.9695401	17.03429013	17.1870188	16.63999441	16.75221747	17.22952124	17.48763696	17.77733346	18.42992955	18.06039021	17.64261164	
MG 18:0_B	18.70461769	18.22174122	18.73284749	19.66581248	18.15931294	18.92510448	18.86381968	18.76505902	18.41763447	19.59553666	19.5809481	19.74340984	19.85111153	19.31866154	18.7194758	18.38084055	18.09202291	18.25649197	18.55083149	19.25819383	19.73097624	20.25594906	19.09964163	19.26623164	
MG 21:0	21.48713831	18.93116007	20.80919182	21.36823716	19.88885137	20.05333331	20.50586787	20.14925725	19.12210905	21.97790122	20.3005911	20.30343428	20.71048024	19.83562776	18.68801548	19.7720829	19.09836096	18.90163685	19.64431537	20.54311966	21.26204055	20.97167471	20.49467895	20.01275076	
PC 34:0|PC 16:0_18:0	23.5127807	23.52166631	23.52969626	23.34089294	23.45062615	23.46828826	23.36556171	23.57277926	23.4703393	23.56403263	23.52169245	23.65388437	23.33838966	23.45629499	23.53801113	23.60202589	23.48669453	23.50974897	23.44833612	22.78187792	23.28783614	23.61250071	23.46246995	23.57289221	
PC 34:1|PC 16:0_18:1	26.67043614	26.59862455	26.5494665	26.66147282	26.39621735	26.5496463	26.38182028	26.49713367	26.63560357	26.80163366	26.493833	26.67199653	26.76179377	26.60880911	26.79461652	26.79393242	26.7986523	26.63186688	26.35429096	25.86317213	26.65153425	26.80146681	26.66743345	26.75801462	
PC 34:2|PC 16:0_18:2	26.6810426	26.6645402	26.26345986	26.64995166	26.54466217	26.66353809	26.71735794	27.01540278	26.86560923	26.8476831	26.77737986	26.7363987	26.91834685	26.74816512	26.86514056	27.00992129	26.89940709	26.64366358	26.57421906	26.06862538	26.73099507	26.89319829	27.01903954	26.9588361	
PC 34:3|PC 16:1_18:2	23.1432121	23.23109701	22.88762853	22.75051123	23.20911221	23.09066478	23.02762355	23.28149259	23.19690955	23.02387249	23.24046327	23.17751261	23.08932221	22.9795383	23.5063044	23.70501766	23.50949037	23.28300507	23.14497579	22.06873962	22.91489654	22.82878709	23.17469717	23.50397933	
PC 36:1|PC 16:0_20:1	25.0945906	25.1671196	25.53716364	25.07811728	25.28020273	25.12909767	25.02291223	25.13424885	25.22701127	25.1040744	25.02861211	25.26375103	25.05015903	24.9748071	25.17333682	25.28204472	25.14341165	25.11594357	24.95892973	24.2361304	24.86505763	24.96575639	25.02551619	25.28491176	
PC 36:2|PC 18:1_18:1	26.9152523	26.963359	26.58773311	26.87730055	26.74691512	26.93405194	26.8801382	27.03518123	26.97910647	27.00581208	26.91661834	26.84040043	27.08669117	27.01947949	27.10380802	27.13615011	27.00560312	26.95098265	26.84092196	26.21830397	26.88877937	26.96040895	27.08685951	27.19941324	
PC 36:3|PC 18:1_18:2	26.59860334	26.56692232	26.26898549	26.28564716	26.48904672	26.58184405	26.52979936	26.75383224	26.61182022	26.5648721	26.58638311	26.58775936	26.55544342	26.49927118	26.9325358	26.95332173	26.74618416	26.73649147	26.48844836	25.51663499	26.36610817	26.34752818	26.54527708	26.7616027	
PC 36:4|PC 16:0_20:4	25.61084323	25.68503426	25.44556801	25.40904858	25.48406648	25.61759613	25.41098971	25.71929881	25.65798058	25.65735862	25.48263234	25.65593098	25.3272032	25.74856202	25.71627165	25.7518121	25.80480241	25.62537138	25.31790862	24.65810865	25.59122823	25.94749587	25.36876325	25.69537257	
PC 36:4|PC 18:2_18:2	24.158712	24.35847935	23.8568555	24.03828437	24.25101741	24.33706387	24.58305604	24.75445879	24.50999206	24.47379789	24.40508732	24.22109346	24.36538342	24.28535753	24.63467549	24.65122121	24.52245878	24.38241712	24.286029	23.34867223	24.21276344	24.28503925	24.61232203	24.7435937	
PC 38:4|PC 18:0_20:4	25.37897654	25.42449561	25.25814708	25.25417907	25.27682152	25.38978956	25.24092048	25.4900622	25.48913733	25.4757087	25.23323471	25.3187409	25.0486598	25.44621687	25.36558653	25.51009718	25.51598687	25.32620059	25.0030703	24.31547927	25.21408592	25.44051308	25.09549102	25.23474965	
PC 38:4|PC 18:2_20:2	24.56262894	24.77292253	24.32082907	24.19922456	24.77356746	24.82951248	24.88475952	25.22343315	24.88191	24.75931157	24.82271816	24.68086058	24.44835578	24.23684201	25.03170397	25.09085191	24.87563835	24.88103428	24.61655473	23.33621574	24.36655389	24.27141368	24.71260255	24.99267544	
PC 38:5|PC 18:1_20:4	24.674047	24.57312174	24.60032564	24.41583927	24.53118451	24.64608798	24.35963361	24.60613558	24.63125543	24.52958047	24.43012055	24.56369792	24.42352746	24.60491072	24.73921258	24.92236143	24.88645902	24.70942648	24.28297437	23.67852078	24.4462827	24.63919562	24.19347077	24.41890649	
PC O-30:0|PC O-16:0_14:0	25.24892473	24.98904682	24.64255516	24.40720364	24.94174976	24.87966788	24.96324187	25.21666313	25.11598637	24.65175486	25.19130769	25.22037572	25.04739188	24.14083541	25.33325014	25.4794229	25.12450334	25.26345987	24.86311933	23.44163259	24.31353425	23.96941276	24.8386625	25.17315298	
PC O-31:0|PC O-16:0_15:0	23.12175434	23.05351851	22.87465533	23.22835241	22.62034785	23.11683532	23.32497074	23.36836322	23.43401072	23.37026814	23.30201112	23.59863529	23.87280875	23.17946021	23.57400825	23.32637734	23.4025948	23.24377136	23.36114367	22.80012106	22.87377842	22.78721877	23.33960276	23.41121434	
PC O-32:0|PC O-16:0_16:0	27.38037854	27.34581454	27.17750947	27.52566413	27.04413342	27.33739907	27.41708761	27.50581275	27.53810309	27.5220027	27.43218366	27.70008631	28.03146973	27.48412838	27.68077752	27.6383543	27.48290586	27.39581288	27.42804771	26.93025708	27.04227261	27.05387263	27.34110782	27.558491	
PC O-32:1|PC O-16:0_16:1	25.44824131	25.42168295	25.28530839	25.35885782	25.27910328	25.22215964	25.28570338	25.45455887	25.51513068	25.30406226	25.54258743	25.82411165	25.98283129	25.36801974	25.85021347	25.89308475	25.70085081	25.6228253	25.47275432	24.87062605	25.0218223	24.99958326	25.43228627	25.69424174	
PC O-33:0|PC O-17:0_16:0	22.40401722	22.31181837	22.1271105	22.31804128	22.11633077	22.39950255	22.48668336	22.52171926	22.53056094	22.31864429	22.49457133	22.65607011	22.79257671	22.21908846	22.73837306	22.58254565	22.64010921	22.49454391	22.37737667	21.66001883	21.5787738	21.63732931	22.27860458	22.46945242	
PC O-34:0|PC O-18:0_16:0	25.48154287	25.42621964	25.13496314	25.32644119	25.07322852	25.37989439	25.44724084	25.69309485	25.41847597	25.44409184	25.51777884	25.54483266	25.74225874	25.38289868	25.71218203	25.47276614	25.47949085	25.3767097	25.0944319	24.45957792	24.8685273	24.90111163	25.42994778	25.53826232	
PC O-34:2|PC O-16:0_18:2	26.73916642	26.69635016	26.5783428	26.89879216	26.44550625	26.74461214	26.98753301	27.01339245	27.01898545	27.00684099	26.94510764	27.15022223	27.51670011	26.92752197	27.22123978	26.99315688	26.89656997	26.72271028	27.00311324	26.56651857	26.80319546	26.85388826	27.18894876	27.28182644	
PC O-35:2|PC O-17:0_18:2	22.32265014	22.16995074	21.95808935	22.13767933	21.98394927	22.22881518	22.2841477	22.56363808	22.47524918	22.28101639	22.40270714	22.4402561	22.78010532	22.17792008	22.80609614	22.5053492	22.57019095	22.43329969	22.3816825	21.6245433	21.76533785	21.78430579	22.37903039	22.60777095	
PC O-36:1|PC O-18:0_18:1	25.13727782	25.03010756	24.71499706	24.91454723	24.71329325	24.86631844	24.9069397	25.1753302	24.98129616	24.81567134	25.12598556	25.13461163	25.35125535	24.98302453	25.36676712	25.20688271	25.19061671	25.06961418	24.58808195	23.88289867	24.39550286	24.34020226	24.96625586	25.13454432	
PC O-36:2|PC O-18:0_18:2	26.74920387	26.60925201	26.54219205	26.79132621	26.4512502	26.63275012	26.65145012	26.78816499	26.71913663	26.67931168	26.73466488	27.02574127	27.27423578	26.76208883	27.07229398	26.91081005	26.81207751	26.75194021	26.66878164	26.01558339	26.26209027	26.3310141	26.81895355	26.94358637	
PC O-36:3|PC O-18:1_18:2	26.48699675	26.34562332	26.17334846	26.60834582	26.08722992	26.29458288	26.35169267	26.51788134	26.49067463	26.55481036	26.38476243	26.6436308	27.1981928	26.50820992	26.84967667	26.73071229	26.67366121	26.5019571	26.64824911	26.06187252	26.19920408	26.26493789	26.6301633	26.74800578	
PC O-36:4|PC O-16:0_20:4	26.38623185	26.3298088	26.15654798	26.34910172	26.16821064	26.28661422	26.39465276	26.62758523	26.44298381	26.51172718	26.49231767	26.57809919	26.72137686	26.46890023	26.7312943	26.62990713	26.45321289	26.41694221	26.26870581	25.82659742	26.29291925	26.4204613	26.45443503	26.63198501	
PC O-36:4|PC O-18:2_18:2	23.33599994	23.14989741	22.99846377	23.44058987	22.91214922	23.30231921	23.45954468	23.64563866	23.33666839	23.28029527	23.27949179	23.44357719	23.98099552	23.45401157	23.57468822	23.51911485	23.27922549	23.2589228	23.64866911	22.91610767	22.96514685	23.14681855	23.52424827	23.48956527	
PC O-38:2|PC O-20:0_18:2	23.86931311	23.79781908	23.42177464	23.70368246	23.50440504	23.69487285	23.84939726	24.09040326	23.92283324	23.77823591	23.90034605	23.90911527	24.17415094	23.63524003	24.11722091	24.02991412	23.9623885	23.89606597	23.24833864	22.42308878	23.22586464	23.21737501	23.91149064	24.05276053	
SM 32:1;2O|SM 14:1;2O/18:0	20.44192896	20.61585782	20.99764437	20.2688286	20.87672205	20.40863581	20.29662986	20.61224689	20.51205386	20.26082744	20.49631833	20.68511399	20.25339211	19.78114738	20.56689956	20.43819334	20.33044657	20.09044348	20.06447345	18.92959131	19.87933558	19.95391234	19.95484434	20.28189226	
SM 33:1;2O|SM 15:1;2O/18:0	21.79076654	21.71396461	21.7985528	21.39901729	21.77999405	21.74488624	21.58583098	21.84605931	21.78957301	21.41077085	21.76282833	21.81022706	21.42382724	20.95527425	21.67974326	21.59584729	21.58064575	21.34763707	21.18501695	20.24697879	20.4402518	20.55285246	21.02697281	21.29145143	
SM 36:2;2O|SM 18:1;2O/18:1	19.08275258	18.94897128	20.79728462	19.51251474	20.03391439	19.25243185	19.64987998	19.66056435	19.78000174	19.48322914	18.96658593	19.54817898	18.99362629	18.84262435	19.06458878	19.03731806	19.03085054	18.96465834	18.53417847	17.75679544	18.70649416	18.91415232	18.83314407	19.04528257	
TG 24:0|TG 8:0_8:0_8:0	17.95359018	18.77749655	18.40381831	19.26043235	18.4875901	18.45516679	19.37407487	18.58955455	18.29335543	19.0754322	18.03374066	18.82791219	19.72697417	19.61537534	18.58592955	19.2759724	17.87589757	17.62183817	20.13561757	21.0701981	20.5096694	20.2945733	19.84633905	19.09095775	
TG 26:0|TG 8:0_8:0_10:0	18.47245296	19.17874486	18.8634452	19.68377639	18.73568541	18.90959175	19.58670003	18.90470447	18.76403658	19.49955337	18.44146116	19.07207977	20.01846908	19.99565226	18.85609853	19.5972662	18.29989198	18.08547363	20.51531854	21.29276816	20.85784491	20.68302048	20.29356324	19.4240118	
TG 36:0|TG 8:0_14:0_14:0	19.14540405	22.1681759	19.3147414	20.37260469	18.89780793	19.4542261	19.74531239	19.35656685	19.5716541	20.13930561	19.13657243	19.87837791	20.62716943	20.47346111	19.5213472	19.34295973	19.28372009	19.23087162	20.17100639	20.49156025	20.51336161	20.93321618	20.47692942	20.06107265	
TG 40:0|TG 10:0_14:0_16:0	18.15921604	20.74514701	18.45891745	19.3140232	18.01195475	18.32872014	19.06003714	18.84205641	18.68517203	19.30858613	18.50577797	18.81814909	19.43240351	19.70727883	18.55060659	18.52959778	18.31727898	18.13858321	19.30227826	19.80166366	19.73189887	20.12475949	19.61487313	19.17085676	
TG 40:1|TG 8:0_14:0_18:1	15.88792393	20.97778863	16.69238392	17.04694191	16.34320019	16.32075183	16.57877264	16.25141143	16.53689238	16.91474404	15.87178089	16.74098239	17.41296877	17.50636479	16.56866241	16.44056112	15.77593299	15.91233551	17.79186588	17.97715542	17.99859468	18.12459961	17.01073682	17.01893173	
TG 42:0|TG 12:0_14:0_16:0	18.10114963	20.46927764	18.4551799	19.30310383	18.24097233	18.51126939	19.13567642	18.61155025	18.63569405	18.99767498	18.23212731	18.85999741	19.48346589	19.64764523	18.65419508	18.65642177	18.22226042	18.18658003	19.67420097	20.48300165	20.05959266	20.16716541	19.47678258	18.9141016	
TG 42:1|TG 8:0_16:0_18:1	18.15014007	20.35666216	18.43730241	19.31387289	18.08882537	18.43909597	18.89132411	18.56701277	18.47304187	19.08916597	18.42281407	18.86548171	19.67357315	19.57006911	18.54112416	18.59476499	18.2715379	18.13369512	19.44166993	20.01615895	19.93991566	20.04899986	19.4228453	19.1734804	
TG 44:0|TG 14:0_14:0_16:0	18.53402704	20.51146264	19.16805719	19.65668901	19.03870921	19.16010261	19.76290173	19.26926706	19.04939918	19.66600692	18.8508788	19.50241321	20.11249036	20.28199654	19.28668888	19.28116155	18.89246641	18.49746559	20.71133189	21.70441637	21.05126576	20.73925072	20.17652635	19.63311247	
TG 44:1|TG 10:0_16:0_18:1	18.86239057	20.4330579	19.04459237	19.88302405	19.08308317	19.26860246	19.6630459	19.1747495	19.17707023	19.61064026	18.87995201	19.37798329	20.20521273	20.21613242	19.35465237	19.38195876	19.00770059	18.70665185	20.48275666	21.35640234	20.86225409	20.78824579	19.99391194	19.73164619	
TG 45:0|TG 14:0_15:0_16:0	18.79939372	19.61050661	19.25192435	19.8990918	19.14608026	19.21223529	19.99247718	19.49709259	19.26348447	19.82675744	19.09671265	19.70226747	20.50759999	20.73816091	19.58300336	19.6643727	19.06494152	18.78314359	20.96804097	21.9733919	21.3390933	21.19289305	20.4853168	19.98450482	
TG 45:1|TG 14:0_15:0_16:1	17.5109381	18.75011357	18.02706752	18.73791343	18.28135868	18.49868711	19.17165903	18.43844507	18.27629029	18.84802022	17.77803572	18.571047	19.31002834	19.61735487	18.73454373	18.93022727	17.95682239	17.47302324	20.33487363	21.66073034	20.66697526	20.22919157	19.45487632	18.78031423	
TG 46:0|TG 14:0_16:0_16:0	19.3118358	20.64888251	20.0297657	20.36160108	19.84533127	19.73791504	20.44356759	19.90883883	20.08142333	20.19185351	19.52809363	20.37208289	20.68871218	21.21237162	20.04815184	20.0421089	19.64023982	19.29506148	21.45030173	22.28666201	21.67609047	21.52429145	20.92590793	20.20977407	
TG 46:1|TG 12:0_16:0_18:1	19.57425036	20.87106277	20.25594923	20.5476992	20.18593797	20.21518215	20.73855735	20.20142881	19.98715139	20.46264781	19.92100899	20.45519017	21.07063689	21.16052717	20.34732535	20.46570559	19.94475454	19.50744072	21.45820149	22.52449283	21.9577326	21.62382982	21.10729765	20.54945357	
TG 47:0|TG 15:0_16:0_16:0	18.8508771	19.6804773	19.66294247	20.013122	19.39850731	19.40106475	20.04131545	19.44634553	19.47971675	20.10172053	18.90029458	19.94275417	20.48638706	20.83851258	19.74621879	19.68672561	19.12404999	18.7085631	21.21085048	22.17241047	21.42878549	21.28680558	20.61677984	20.05043947	
TG 47:1|TG 15:0_16:0_16:1	18.26214672	19.21230835	19.03341551	19.47616231	19.06537221	19.20670791	19.79603393	19.10932239	18.96016473	19.66371894	18.39925157	19.38672027	20.07971213	20.24547967	19.33093079	19.60707295	18.59093862	18.23533854	21.07802415	22.34766855	21.30489298	20.92784568	20.23333461	19.43259742	
TG 48:0|TG 16:0_16:0_16:0	20.7488873	21.21190893	21.13589928	20.98063628	20.95122751	20.85696359	21.14034524	20.94777525	20.96925482	21.04533477	20.8728939	21.16250674	21.28018387	21.62874673	20.87910017	21.09147358	20.74313771	20.40591215	21.73835863	22.52237894	21.98196694	21.98352412	21.47470723	21.35905501	
TG 48:1|TG 14:0_16:0_18:1	20.36339988	21.27101971	21.19714061	21.0728193	20.94734909	20.58198784	21.14663613	20.75633645	20.88370785	20.82481978	20.56123425	20.99742555	21.34405154	21.52088826	20.88527835	20.97638302	20.72296101	20.25470037	21.80997527	22.77895757	22.07903345	21.82745615	21.45571897	20.78742837	
TG 48:2|TG 14:0_16:1_18:1	20.12276262	20.7329396	20.8685193	20.6835517	20.73782195	20.38808661	20.86743239	20.64455849	20.510119	20.30319507	20.35879354	20.76280151	21.31842547	21.29434606	20.84554382	20.82765743	20.4084006	20.05767326	21.39138745	22.31279092	21.83081969	21.67187194	21.25456651	20.82119282	
TG 49:0|TG 15:0_16:0_18:0	18.10382488	18.8521594	18.72038198	19.06818485	18.45218571	18.57292519	19.04155803	18.74702776	18.5361148	19.04174298	18.33479698	19.04484695	19.25915115	19.91384299	18.55540412	18.77068823	18.18129859	17.72847579	20.16708961	20.8338047	20.27443685	20.10724527	19.62945199	18.93229937	
TG 49:1|TG 15:0_16:0_18:1	18.45304045	19.3903439	19.51012756	19.43545508	19.32525754	19.14768195	19.84752769	19.15675524	19.13027977	19.43955469	18.48910077	19.31357657	19.78985895	20.13186211	19.28812981	19.39735491	18.65254594	18.29038781	20.77814784	22.00787807	21.14171775	20.61581274	20.06442444	19.3272142	
TG 49:2|TG 16:0_16:1_17:1	17.83411304	18.65650521	18.60216578	18.72901502	18.68904636	18.64833242	19.23146149	18.56010217	18.41891488	18.89691528	18.13026025	18.5282295	19.29643265	19.40909187	18.79436367	18.99282454	18.04134195	17.83436403	20.0136586	21.41147645	20.47329593	20.09161369	19.3580647	18.8368242	
TG 50:0|TG 16:0_16:0_18:0	20.13379984	20.39406294	20.32059434	20.32089591	20.30083069	20.20737954	20.4947518	20.39449502	20.36762276	20.22110655	20.318224	20.39719132	20.6628957	20.92954493	19.86270334	19.91444604	19.94286871	19.81668589	21.02826303	21.26121422	21.15816436	21.23357045	20.78662457	20.4851043	
TG 50:1|TG 16:0_16:0_18:1	21.96796049	22.19374521	22.45627293	22.08158767	22.17309045	21.96406885	22.14769303	21.9711838	21.99593513	21.94349675	22.03270606	22.0803377	22.23138883	22.34502418	22.0706227	22.10785174	21.90102942	21.61180646	22.27789803	22.65896231	22.39117669	22.39713733	22.04922498	22.18458531	
TG 50:2|TG 16:0_16:1_18:1	21.86522374	22.06885826	22.3930882	22.12853595	22.1795258	21.88313842	22.27422639	22.04716964	22.16600463	21.87975054	21.92071927	22.01295148	22.411079	22.36041529	22.08824706	22.19151615	22.0348607	21.67201913	22.41043858	22.73900745	22.54942202	22.55057375	22.46665831	22.15330536	
TG 50:3|TG 16:0_16:1_18:2	20.22599464	20.31112779	20.89655897	20.20672912	20.80690246	19.96370573	20.43099347	20.35499351	20.51730295	19.94334543	20.22667529	20.22111503	20.64963396	20.5806673	20.66221989	20.65651905	20.38782288	20.19478586	20.84405204	21.19326655	20.87169032	20.71181657	20.61816093	20.36709827	
TG 52:0|TG 16:0_18:0_18:0	18.93252969	19.18499857	18.90745843	19.18626143	18.73139295	18.75063133	19.4873157	18.98025448	19.33299189	18.88241777	19.08235048	18.91402866	19.64840324	19.98915603	18.69112549	18.47336204	18.63337489	18.6746217	19.78964604	20.64463297	20.06193751	20.13344633	19.76536755	19.50848151	
TG 52:1|TG 16:0_18:0_18:1	21.33252517	21.26936171	21.48735007	21.48723224	21.25563103	21.35754313	21.49448601	21.13188593	21.39850134	21.2235111	21.46172579	21.37252615	21.70533766	21.79030976	21.08745283	21.04313428	21.0536251	21.00222301	21.55886828	21.62320809	21.54891772	21.87252421	21.58312556	21.18688088	
TG 52:2|TG 16:0_18:1_18:1	22.89682081	22.92681682	23.18209455	23.07312734	22.78656669	22.89213037	22.82649734	22.70122254	22.58226497	22.75310378	22.68936864	22.82224261	23.14288203	23.1935984	22.75642261	22.87231038	22.61774253	22.4482185	22.93103837	23.09604892	23.02632	23.17603251	22.87218829	23.07262117	
TG 52:3|TG 16:0_18:1_18:2	22.7681514	22.75110193	23.06310418	22.90881011	22.76704789	22.77860895	22.8275711	22.65461066	22.757887	22.57860149	22.57966227	22.51192581	23.05977436	22.93197113	22.73911089	22.70214396	22.74577932	22.47437066	22.75151734	22.84945315	22.95850152	23.08718434	23.09814105	22.87823258	
TG 52:4|TG 16:0_18:2_18:2	21.98662519	21.91019274	22.26312961	22.22513862	22.04780165	21.86710809	22.24365418	22.16241136	22.11685888	22.08997689	22.07207306	22.0872487	22.674609	22.64321261	22.18702784	22.13211511	21.8116697	21.84163576	22.22413751	22.5453777	22.49610362	22.75530542	22.54314948	22.4554651	
TG 54:2|TG 18:0_18:1_18:1	21.54911485	21.39384295	21.53944735	21.62583053	21.26782473	21.56065152	21.4513656	21.25387309	21.29802555	21.23319809	21.388855	21.38807713	21.8276192	21.83823795	21.18278637	21.13708478	21.09624339	21.08737557	21.53756753	21.4770492	21.59990245	21.90242233	21.63569176	21.33663131	
TG 54:3|TG 18:1_18:1_18:1	22.83408257	22.82686735	22.8976776	23.06654598	22.49968552	22.86934656	22.64948173	22.55051694	22.45987587	22.68329599	22.56789644	22.65457616	23.18139599	23.14091933	22.69576612	22.68510599	22.54323593	22.35070395	22.82346239	23.02553496	23.01387475	23.1561165	22.7979742	22.99312636	
TG 54:4|TG 18:1_18:1_18:2	22.87973028	22.76634574	22.95100535	23.07764268	22.57680856	22.89906811	22.80936714	22.5741296	22.67508789	22.65414601	22.46973205	22.57239154	23.22597754	22.98951962	22.77348502	22.73769003	22.71753123	22.37333802	22.72236847	22.89102023	22.97806925	23.13033683	23.15608282	22.99379238	
TG 54:5|TG 18:1_18:2_18:2	22.31222694	22.25675464	22.49485748	22.77729394	22.07195484	22.30885986	22.57941777	22.41026573	22.11767036	22.50951445	22.32903458	22.45845248	23.1074697	23.06995525	22.38112513	22.52474565	22.19619852	22.08980572	22.52929534	22.84868304	22.80854209	23.13660494	22.91669144	22.77332025	
TG 54:6|TG 18:2_18:2_18:2	21.35211576	21.55563105	21.78829141	22.30224164	21.39843476	21.71610999	22.1249648	21.98329335	21.91303221	22.16172997	21.69523765	21.87682833	22.7204917	22.52650484	21.69210036	21.7045877	21.59537732	21.42024172	22.07103438	22.58485458	22.33985035	22.95848436	22.43645361	22.29690257	
TG 56:3|TG 18:0_20:1_18:2	20.05770673	19.63305489	19.72275241	19.72222617	19.60208028	20.00679051	19.64113096	19.37937189	19.48573934	19.2749689	19.64753575	19.63531369	19.78246213	19.75333569	19.40981279	19.42731036	19.45442081	19.58981376	19.75145656	19.27544122	19.4669294	19.39708585	19.64787643	19.52071192	
TG 56:4|TG 18:0_18:1_20:3	21.01301364	20.6484061	20.66297821	20.34313188	20.50973136	20.71953718	20.1708784	20.38504237	20.38384569	20.06450754	20.51418819	20.33366235	20.39955084	20.35765008	20.42962483	20.6023407	20.47239495	20.39963149	20.34876107	19.71254066	20.15474001	20.07585377	20.29904903	20.65084307	
TG 56:7|TG 18:1_18:2_20:4	20.37352021	20.22658415	20.27311207	19.82454328	20.16247192	20.08594312	20.24862012	20.1400757	19.97386472	19.67157083	20.22231606	20.01201326	20.35053793	19.72550899	20.51893545	20.53407494	20.21468999	20.01765915	20.46995778	19.73409847	20.16987755	19.77788709	20.29589411	20.40193852	
TG 56:8|TG 16:0_18:2_22:6	19.08059348	18.98079542	18.92333762	18.7176973	19.11422139	18.78206088	19.30042887	19.08907028	19.28769296	18.46573835	19.05431226	18.69745686	19.212896	18.62758711	19.51243221	19.34185703	19.06826527	19.017591	19.55549168	18.98884347	19.18768582	18.82438413	19.23787773	19.56743316	
TG 58:6|TG 18:1_18:1_22:4	20.19190924	19.70656847	19.88295705	19.46335483	19.6788311	19.75498717	19.17416958	19.34246566	19.48133189	19.15834299	19.55539108	19.42409797	19.57739041	19.24238841	19.57504117	19.44507611	19.51272214	19.55880867	19.45288497	18.62930222	19.28637587	18.93103212	19.54316424	19.53974746	
MS_METABOLITE_DATA_END
#METABOLITES
METABOLITES_START
metabolite_name	KEGG	PUBCHEM
Adenosine 5'-monophosphate	C00020	6083
CAR 16:0	C02990	462
CAR 18:0	C02301	5282
CAR 18:1	C14641	6441392
CE 18:1	C15441	5283632  
CE 18:2	-	5287939
CE 20:3	-	14274976
CE 20:4	-	164281578 
CE 20:5	-	164483673
CE 22:6_LipPos	-	14274978
Cer 33:3;2O|Cer 18:1;2O/15:2	-	164278814
Cer 34:1;2O|Cer 18:1;2O/16:0	-	154573062
Cer 40:1;2O|Cer 18:1;2O/22:0	-	 154573070 
Cer 42:1;2O|Cer 18:1;2O/24:0_A	-	154573074 
Cer 42:1;2O|Cer 18:1;2O/24:0_B	-	154573074 
Cer 42:2;2O|Cer 18:1;2O/24:1	-	 154573075  
Cer 42:3;2O|Cer 18:1;2O/24:2	-	138144631  
DG 32:0|DG 16:0_16:0	-	45934309  
DG 34:0|DG 16:0_18:0	-	91870182  
DG 34:1|DG 16:0_18:1	-	5282283 
DG 34:2|DG 16:0_18:2	-	102427902 
DG 35:2|DG 20:0_15:2	-	5283472  
DG 36:0|DG 18:0_18:0	-	53477922 
DG 36:1|DG 18:0_18:1	-	91666382  
DG 36:2|DG 18:0_18:2	-	9543716  
DG 36:3|DG 18:1_18:2	-	9543722  
DG 38:4|DG 18:0_20:4	-	53478168
LPC 15:0/0:0	-	24779458 
LPC 18:0/0:0	-	24779513
LPC 18:1	-	16081932  
LPC 18:2	-	11005824  
LPC 19:0	-	13648279  
LPC 20:4	-	53480469 
LPC 22:6	-	10415542 
MG 18:0_A	-	24699  
MG 18:0_B	-	24699  
MG 21:0	-	131779650 
PC 34:0|PC 16:0_18:0	-	24778686  
PC 34:1|PC 16:0_18:1	-	 5497103  
PC 34:2|PC 16:0_18:2	-	5287971  
PC 34:3|PC 16:1_18:2	-	52923213  
PC 36:1|PC 16:0_20:1	-	24778825  
PC 36:2|PC 18:1_18:1	-	10350317  
PC 36:3|PC 18:1_18:2	-	24778937  
PC 36:4|PC 16:0_20:4	-	52922783
PC 36:4|PC 18:2_18:2	-	52922783
PC 38:4|PC 18:0_20:4	-	16219824  
PC 38:4|PC 18:2_20:2	-	16219824  
PC 38:5|PC 18:1_20:4	-	52923235 
PC O-30:0|PC O-16:0_14:0	-	164399195 
PC O-31:0|PC O-16:0_15:0	-	164334893  
PC O-32:0|PC O-16:0_16:0	-	252150170
PC O-32:1|PC O-16:0_16:1	-	164329719  
PC O-33:0|PC O-17:0_16:0	-	164333045  
PC O-34:0|PC O-18:0_16:0	-	11803170 
PC O-34:2|PC O-16:0_18:2	-	164457830 
PC O-35:2|PC O-17:0_18:2	-	164477664 
PC O-36:1|PC O-18:0_18:1	-	164286087  
PC O-36:2|PC O-18:0_18:2	-	164337328  
PC O-36:3|PC O-18:1_18:2	-	164257340
PC O-36:4|PC O-16:0_20:4	-	164195666 
PC O-36:4|PC O-18:2_18:2	-	164195666 
PC O-38:2|PC O-20:0_18:2	-	164306357  
SM 32:1;2O|SM 14:1;2O/18:0	-	164233492  
SM 33:1;2O|SM 15:1;2O/18:0	-	70679243
SM 36:2;2O|SM 18:1;2O/18:1	-	 154573096 
TG 24:0|TG 8:0_8:0_8:0	-	131758038  
TG 26:0|TG 8:0_8:0_10:0	-	433786476  
TG 36:0|TG 8:0_14:0_14:0	-	10851  
TG 40:0|TG 10:0_14:0_16:0	-	91865738 
TG 40:1|TG 8:0_14:0_18:1	-	56936435 
TG 42:0|TG 12:0_14:0_16:0	-	 11148 
TG 42:1|TG 8:0_16:0_18:1	-	56936439 
TG 44:0|TG 14:0_14:0_16:0	-	56936498 
TG 44:1|TG 10:0_16:0_18:1	-	56937112 
TG 45:0|TG 14:0_15:0_16:0	-	56936524 
TG 45:1|TG 14:0_15:0_16:1	-	56938362 
TG 46:0|TG 14:0_16:0_16:0	-	4099879
TG 46:1|TG 12:0_16:0_18:1	-	56936454 
TG 47:0|TG 15:0_16:0_16:0	-	56936694 
TG 47:1|TG 15:0_16:0_16:1	-	56936564
TG 48:0|TG 16:0_16:0_16:0	-	91865742  
TG 48:1|TG 14:0_16:0_18:1	-	9543986 
TG 48:2|TG 14:0_16:1_18:1	-	9543987
TG 49:0|TG 15:0_16:0_18:0	-	56936529  
TG 49:1|TG 15:0_16:0_18:1	-	9543991 
TG 49:2|TG 16:0_16:1_17:1	-	9543993
TG 50:0|TG 16:0_16:0_18:0	-	545588  
TG 50:1|TG 16:0_16:0_18:1	-	11308890  
TG 50:2|TG 16:0_16:1_18:1	-	9544010
TG 50:3|TG 16:0_16:1_18:2	-	25240357 
TG 52:0|TG 16:0_18:0_18:0	-	545690  
TG 52:1|TG 16:0_18:0_18:1	-	5365005
TG 52:2|TG 16:0_18:1_18:1	-	 56936476  
TG 52:3|TG 16:0_18:1_18:2	-	56938177  
TG 52:4|TG 16:0_18:2_18:2	-	25240364 
TG 54:2|TG 18:0_18:1_18:1	-	56938183
TG 54:3|TG 18:1_18:1_18:1	-	5497163 
TG 54:4|TG 18:1_18:1_18:2	-	9544255
TG 54:5|TG 18:1_18:2_18:2	-	9544294  
TG 54:6|TG 18:2_18:2_18:2	-	5322095  
TG 56:3|TG 18:0_20:1_18:2	-	9544447  
TG 56:4|TG 18:0_18:1_20:3	-	25240379
TG 56:7|TG 18:1_18:2_20:4	-	9544695 
TG 56:8|TG 16:0_18:2_22:6	-	56939941  
TG 58:6|TG 18:1_18:1_22:4	-	9544977 
METABOLITES_END
#END