#METABOLOMICS WORKBENCH maloyan_20240819_120746 DATATRACK_ID:5121 STUDY_ID:ST003426 ANALYSIS_ID:AN005626 PROJECT_ID:PR002118
VERSION             	1
CREATED_ON             	August 22, 2024, 9:29 am
#PROJECT
PR:PROJECT_TITLE                 	Metabolomic and transcriptomic remodeling of bone marrow myeloid cells in
PR:PROJECT_TITLE                 	response to maternal obesity
PR:PROJECT_SUMMARY               	Maternal obesity puts the offspring at high risk of developing obesity and
PR:PROJECT_SUMMARY               	cardio-metabolic diseases in adulthood. Here, using a mouse model of maternal
PR:PROJECT_SUMMARY               	high-fat diet-induced obesity, we show that whole body fat content of the
PR:PROJECT_SUMMARY               	Off-HFD increases significantly from very early age when compared to the Off-RD.
PR:PROJECT_SUMMARY               	We have previously shown significant metabolic and immune perturbations in the
PR:PROJECT_SUMMARY               	bone marrow of newly weaned offspring of obese mothers. Therefore, we
PR:PROJECT_SUMMARY               	hypothesized that lipid metabolism is altered in the bone marrow Off-HFD in
PR:PROJECT_SUMMARY               	newly weaned offspring of obese mothers when compared to the Off-RD. To test
PR:PROJECT_SUMMARY               	this hypothesis, we investigated the lipidomic profile of bone marrow cells
PR:PROJECT_SUMMARY               	collected from three-week-old offspring of regular and high fat diet-fed
PR:PROJECT_SUMMARY               	mothers. Diacylgycerols (DAGs), triacylglycerols (TAGs), sphingolipids and
PR:PROJECT_SUMMARY               	phospholipids, including plasmalogen, and lysophospholipids were remarkably
PR:PROJECT_SUMMARY               	different between the groups, independent of fetal sex. Levels of cholesteryl
PR:PROJECT_SUMMARY               	esters were significantly decreased in offspring of obese mothers, suggesting
PR:PROJECT_SUMMARY               	reduced delivery of cholesterol to bone marrow cells. This was accompanied by
PR:PROJECT_SUMMARY               	age-dependent progression of mitochondrial dysfunction in bone marrow cells. We
PR:PROJECT_SUMMARY               	subsequently isolated CD11b+ myeloid cells from three-week-old mice and
PR:PROJECT_SUMMARY               	conducted metabolomics, lipidomics, and transcriptomics analyses. The lipidomic
PR:PROJECT_SUMMARY               	profiles of these bone marrow myeloid cells were largely similar to that seen in
PR:PROJECT_SUMMARY               	bone marrow cells and included increases in DAGs and phospholipids alongside
PR:PROJECT_SUMMARY               	decreased TAGs, except for long-chain TAGs, which were significantly increased.
PR:PROJECT_SUMMARY               	Our data also revealed significant sex-dependent changes in amino acids and
PR:PROJECT_SUMMARY               	metabolites related to energy metabolism. Transcriptomic analysis revealed
PR:PROJECT_SUMMARY               	altered expression of genes related to major immune pathways including
PR:PROJECT_SUMMARY               	macrophage alternative activation, B-cell receptor signaling, TGF signaling,
PR:PROJECT_SUMMARY               	and communication between the innate and adaptive immune systems. All told, this
PR:PROJECT_SUMMARY               	study revealed lipidomic, metabolomic, and gene expression abnormalities in bone
PR:PROJECT_SUMMARY               	marrow cells broadly, and in bone marrow myeloid cells particularly, in the
PR:PROJECT_SUMMARY               	newly-weaned offspring of obese mothers, which might at least partially explain
PR:PROJECT_SUMMARY               	the progression of metabolic and cardiovascular diseases in their adulthood.
PR:INSTITUTE                     	Oregon Health and Science University
PR:DEPARTMENT                    	Knight Cardiovascular Institute
PR:LABORATORY                    	Maloyan Laboratory
PR:LAST_NAME                     	Maloyan
PR:FIRST_NAME                    	Alina
PR:ADDRESS                       	3181 S.W. Sam Jackson Park Road, Portland, Oregon, 97239-3098, USA
PR:EMAIL                         	maloyan@ohsu.edu
PR:PHONE                         	513-238-3783
#STUDY
ST:STUDY_TITLE                   	Metabolomic and transcriptomic remodeling of bone marrow myeloid cells in
ST:STUDY_TITLE                   	response to maternal obesity
ST:STUDY_SUMMARY                 	Maternal obesity puts the offspring at high risk of developing obesity and
ST:STUDY_SUMMARY                 	cardio-metabolic diseases in adulthood. Here, using a mouse model of maternal
ST:STUDY_SUMMARY                 	high-fat diet-induced obesity, we show that whole body fat content of the
ST:STUDY_SUMMARY                 	Off-HFD increases significantly from very early age when compared to the Off-RD.
ST:STUDY_SUMMARY                 	We have previously shown significant metabolic and immune perturbations in the
ST:STUDY_SUMMARY                 	bone marrow of newly weaned offspring of obese mothers. Therefore, we
ST:STUDY_SUMMARY                 	hypothesized that lipid metabolism is altered in the bone marrow Off-HFD in
ST:STUDY_SUMMARY                 	newly weaned offspring of obese mothers when compared to the Off-RD. To test
ST:STUDY_SUMMARY                 	this hypothesis, we investigated the lipidomic profile of bone marrow cells
ST:STUDY_SUMMARY                 	collected from three-week-old offspring of regular and high fat diet-fed
ST:STUDY_SUMMARY                 	mothers. Diacylgycerols (DAGs), triacylglycerols (TAGs), sphingolipids and
ST:STUDY_SUMMARY                 	phospholipids, including plasmalogen, and lysophospholipids were remarkably
ST:STUDY_SUMMARY                 	different between the groups, independent of fetal sex. Levels of cholesteryl
ST:STUDY_SUMMARY                 	esters were significantly decreased in offspring of obese mothers, suggesting
ST:STUDY_SUMMARY                 	reduced delivery of cholesterol to bone marrow cells. This was accompanied by
ST:STUDY_SUMMARY                 	age-dependent progression of mitochondrial dysfunction in bone marrow cells. We
ST:STUDY_SUMMARY                 	subsequently isolated CD11b+ myeloid cells from three-week-old mice and
ST:STUDY_SUMMARY                 	conducted metabolomics, lipidomics, and transcriptomics analyses. The lipidomic
ST:STUDY_SUMMARY                 	profiles of these bone marrow myeloid cells were largely similar to that seen in
ST:STUDY_SUMMARY                 	bone marrow cells and included increases in DAGs and phospholipids alongside
ST:STUDY_SUMMARY                 	decreased TAGs, except for long-chain TAGs, which were significantly increased.
ST:STUDY_SUMMARY                 	Our data also revealed significant sex-dependent changes in amino acids and
ST:STUDY_SUMMARY                 	metabolites related to energy metabolism. Transcriptomic analysis revealed
ST:STUDY_SUMMARY                 	altered expression of genes related to major immune pathways including
ST:STUDY_SUMMARY                 	macrophage alternative activation, B-cell receptor signaling, TGF signaling,
ST:STUDY_SUMMARY                 	and communication between the innate and adaptive immune systems. All told, this
ST:STUDY_SUMMARY                 	study revealed lipidomic, metabolomic, and gene expression abnormalities in bone
ST:STUDY_SUMMARY                 	marrow cells broadly, and in bone marrow myeloid cells particularly, in the
ST:STUDY_SUMMARY                 	newly-weaned offspring of obese mothers, which might at least partially explain
ST:STUDY_SUMMARY                 	the progression of metabolic and cardiovascular diseases in their adulthood.
ST:INSTITUTE                     	Oregon Health and Science University
ST:LABORATORY                    	Maloyan Laboratory
ST:LAST_NAME                     	Maloyan
ST:FIRST_NAME                    	Alina
ST:ADDRESS                       	3181 S.W. Sam Jackson Park Road, Portland, Oregon, 97239-3098, USA
ST:EMAIL                         	maloyan@ohsu.edu
ST:PHONE                         	513-238-3783
#SUBJECT
SU:SUBJECT_TYPE                  	Mammal
SU:SUBJECT_SPECIES               	Mus musculus
SU:TAXONOMY_ID                   	10090
SU:AGE_OR_AGE_RANGE              	3 week old
SU:GENDER                        	Male and female
SU:ANIMAL_HOUSING                	regular
SU:ANIMAL_FEED                   	Fed by a regular or high fat diet
SU:ANIMAL_WATER                  	ad libitum
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data
SUBJECT_SAMPLE_FACTORS           	-	Maloyan_Asth_FOH2_l_20	Offspring group:Offspring of High-Fat-Diet | Sex:Female | Sample source:Bone marrow	RAW_FILE_NAME=Maloyan_Asth_FOH2_l_20
SUBJECT_SAMPLE_FACTORS           	-	Maloyan_Asth_FOH3_l_21	Offspring group:Offspring of High-Fat-Diet | Sex:Female | Sample source:Bone marrow	RAW_FILE_NAME=Maloyan_Asth_FOH3_l_21
SUBJECT_SAMPLE_FACTORS           	-	Maloyan_Asth_FOH4_l_22	Offspring group:Offspring of High-Fat-Diet | Sex:Female | Sample source:Bone marrow	RAW_FILE_NAME=Maloyan_Asth_FOH4_l_22
SUBJECT_SAMPLE_FACTORS           	-	Maloyan_Asth_FOH5_l_23	Offspring group:Offspring of High-Fat-Diet | Sex:Female | Sample source:Bone marrow	RAW_FILE_NAME=Maloyan_Asth_FOH5_l_23
SUBJECT_SAMPLE_FACTORS           	-	Maloyan_Asth_FOH6_l_24	Offspring group:Offspring of High-Fat-Diet | Sex:Female | Sample source:Bone marrow	RAW_FILE_NAME=Maloyan_Asth_FOH6_l_24
SUBJECT_SAMPLE_FACTORS           	-	Maloyan_Asth_FOR1_l_13	Offspring group:Offspring of Regular Diet | Sex:Female | Sample source:Bone marrow	RAW_FILE_NAME=Maloyan_Asth_FOR1_l_13
SUBJECT_SAMPLE_FACTORS           	-	Maloyan_Asth_FOR2_l_14	Offspring group:Offspring of Regular Diet | Sex:Female | Sample source:Bone marrow	RAW_FILE_NAME=Maloyan_Asth_FOR2_l_14
SUBJECT_SAMPLE_FACTORS           	-	Maloyan_Asth_FOR3_l_15	Offspring group:Offspring of Regular Diet | Sex:Female | Sample source:Bone marrow	RAW_FILE_NAME=Maloyan_Asth_FOR3_l_15
SUBJECT_SAMPLE_FACTORS           	-	Maloyan_Asth_FOR4_l_16	Offspring group:Offspring of Regular Diet | Sex:Female | Sample source:Bone marrow	RAW_FILE_NAME=Maloyan_Asth_FOR4_l_16
SUBJECT_SAMPLE_FACTORS           	-	Maloyan_Asth_FOR5_l_17	Offspring group:Offspring of Regular Diet | Sex:Female | Sample source:Bone marrow	RAW_FILE_NAME=Maloyan_Asth_FOR5_l_17
SUBJECT_SAMPLE_FACTORS           	-	Maloyan_Asth_FOR6_l_18	Offspring group:Offspring of Regular Diet | Sex:Female | Sample source:Bone marrow	RAW_FILE_NAME=Maloyan_Asth_FOR6_l_18
SUBJECT_SAMPLE_FACTORS           	-	Maloyan_Asth_MOH1_l_07	Offspring group:Offspring of High-Fat-Diet | Sex:Male | Sample source:Bone marrow	RAW_FILE_NAME=Maloyan_Asth_MOH1_l_07
SUBJECT_SAMPLE_FACTORS           	-	Maloyan_Asth_MOH2_l_08	Offspring group:Offspring of High-Fat-Diet | Sex:Male | Sample source:Bone marrow	RAW_FILE_NAME=Maloyan_Asth_MOH2_l_08
SUBJECT_SAMPLE_FACTORS           	-	Maloyan_Asth_MOH3_l_09	Offspring group:Offspring of High-Fat-Diet | Sex:Male | Sample source:Bone marrow	RAW_FILE_NAME=Maloyan_Asth_MOH3_l_09
SUBJECT_SAMPLE_FACTORS           	-	Maloyan_Asth_MOH4_l_10	Offspring group:Offspring of High-Fat-Diet | Sex:Male | Sample source:Bone marrow	RAW_FILE_NAME=Maloyan_Asth_MOH4_l_10
SUBJECT_SAMPLE_FACTORS           	-	Maloyan_Asth_MOH5_l_11	Offspring group:Offspring of High-Fat-Diet | Sex:Male | Sample source:Bone marrow	RAW_FILE_NAME=Maloyan_Asth_MOH5_l_11
SUBJECT_SAMPLE_FACTORS           	-	Maloyan_Asth_MOH6_l_12	Offspring group:Offspring of High-Fat-Diet | Sex:Male | Sample source:Bone marrow	RAW_FILE_NAME=Maloyan_Asth_MOH6_l_12
SUBJECT_SAMPLE_FACTORS           	-	Maloyan_Asth_MOR1_l_01	Offspring group:Offspring of Regular Diet | Sex:Male | Sample source:Bone marrow	RAW_FILE_NAME=Maloyan_Asth_MOR1_l_01
SUBJECT_SAMPLE_FACTORS           	-	Maloyan_Asth_MOR2_l_02	Offspring group:Offspring of Regular Diet | Sex:Male | Sample source:Bone marrow	RAW_FILE_NAME=Maloyan_Asth_MOR2_l_02
SUBJECT_SAMPLE_FACTORS           	-	Maloyan_Asth_MOR3_l_03	Offspring group:Offspring of Regular Diet | Sex:Male | Sample source:Bone marrow	RAW_FILE_NAME=Maloyan_Asth_MOR3_l_03
SUBJECT_SAMPLE_FACTORS           	-	Maloyan_Asth_MOR4_l_04	Offspring group:Offspring of Regular Diet | Sex:Male | Sample source:Bone marrow	RAW_FILE_NAME=Maloyan_Asth_MOR4_l_04
SUBJECT_SAMPLE_FACTORS           	-	Maloyan_Asth_MOR5_l_05	Offspring group:Offspring of Regular Diet | Sex:Male | Sample source:Bone marrow	RAW_FILE_NAME=Maloyan_Asth_MOR5_l_05
SUBJECT_SAMPLE_FACTORS           	-	Maloyan_Asth_MOR6_l_06	Offspring group:Offspring of Regular Diet | Sex:Male | Sample source:Bone marrow	RAW_FILE_NAME=Maloyan_Asth_MOR6_l_06
SUBJECT_SAMPLE_FACTORS           	1	Maloyan_Asth_FOH1_l_19	Offspring group:Offspring of High-Fat-Diet | Sex:Female | Sample source:Bone marrow	RAW_FILE_NAME=Maloyan_Asth_FOH1_l_19
#COLLECTION
CO:COLLECTION_SUMMARY            	Bone marrow cells were isolated as previously reported (20). Briefly, femurs
CO:COLLECTION_SUMMARY            	were flushed using a 25G5/8 needle with medium containing RPMI1640 + 2% FBS + 10
CO:COLLECTION_SUMMARY            	units/ml heparin + penicillin and streptomycin. Bits of bone were removed using
CO:COLLECTION_SUMMARY            	a sterile 4 mm nylon cell strainer (Falcon 352340). Cells were dissolved in 50
CO:COLLECTION_SUMMARY            	ml medium and centrifuged at 2000 rpm (900 x g) for ten minutes at 4 °C. The
CO:COLLECTION_SUMMARY            	resultant cell pellets were washed twice with 50 ml of serum-free RPMI (RPMI1640
CO:COLLECTION_SUMMARY            	+ 20 mM Hepes + penicillin and streptomycin, adjusted to pH 7.4 before
CO:COLLECTION_SUMMARY            	filtering), centrifuged at 2000 rpm at 4 °C for five minutes, and finally
CO:COLLECTION_SUMMARY            	resuspended in 25 ml of serum-free RPMI. The obtained bone marrow cells were
CO:COLLECTION_SUMMARY            	enriched for cells expressing CD11b using anti-CD11b magnetic beads (STEM CELL
CO:COLLECTION_SUMMARY            	Technologies, Vancouver, BC), applied according to the manufacturer’s
CO:COLLECTION_SUMMARY            	instructions. Isolated CD11b+ cells were sent for metabolomic, lipidomic, and
CO:COLLECTION_SUMMARY            	transcriptomic analyses.
CO:COLLECTION_PROTOCOL_FILENAME  	Lipidomics_and_metabolomics.pdf
CO:SAMPLE_TYPE                   	Bone marrow
#TREATMENT
TR:TREATMENT_SUMMARY             	To induce maternal obesity, high-fat diet (Teklad Cat#TD.06415) or its control,
TR:TREATMENT_SUMMARY             	a regular diet (RD, PicoLab® Laboratory Rodent Diet Cat # 5LOD) was given to
TR:TREATMENT_SUMMARY             	virgin female FVB/NJ mice from the six-weeks of age and throughout the entire
TR:TREATMENT_SUMMARY             	study. After eight weeks of dietary intervention, RD- and HFD-fed female mice
TR:TREATMENT_SUMMARY             	were bred to an age-matched RD-fed male. At weaning, male and female offspring
TR:TREATMENT_SUMMARY             	from each mother were randomly selected for the study. The offspring were fed a
TR:TREATMENT_SUMMARY             	regular diet only starting from weaning and throughout their life.
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	Lipids and metabolites were extracted from cells using MPLEx (Metabolite,
SP:SAMPLEPREP_SUMMARY            	Protein, Lipid Extraction). First, 1 mL cold (-20 °C) chloroform:methanol
SP:SAMPLEPREP_SUMMARY            	working mix (prepared 2:1, v/v) was added into a chloroform-compatible 2 mL
SP:SAMPLEPREP_SUMMARY            	Sorenson MulTI™ SafeSeal™ microcentrifuge tube (Sorenson Bioscience, Salt
SP:SAMPLEPREP_SUMMARY            	Lake City, UT) inside an ice-block. The sample and cold water were then added to
SP:SAMPLEPREP_SUMMARY            	make a final ratio of 8:4:3 chloroform:methanol:water/sample, vortexed, allowed
SP:SAMPLEPREP_SUMMARY            	to incubate in the ice block for 15 mins with 2000 rpm shaking, and finally
SP:SAMPLEPREP_SUMMARY            	sonicated in an ice water bath sonicator. Afterwards, the samples were
SP:SAMPLEPREP_SUMMARY            	centrifuged at 12,000 x g for 10 mins to separate the polar and non-polar phases
SP:SAMPLEPREP_SUMMARY            	and the protein interlayer. The upper polar phase and a portion of the lower
SP:SAMPLEPREP_SUMMARY            	non-polar phase were allotted to metabolite samples, with the remaining lower
SP:SAMPLEPREP_SUMMARY            	non-polar phase used for lipid samples. The whole process was repeated once on
SP:SAMPLEPREP_SUMMARY            	the protein interlayer to ensure complete removal of metabolites and lipids. The
SP:SAMPLEPREP_SUMMARY            	protein was then washed with 500 µl of cold 100% methanol and centrifuged for
SP:SAMPLEPREP_SUMMARY            	five minutes to pellet the protein, and the wash layer was added to the
SP:SAMPLEPREP_SUMMARY            	metabolites vial. Metabolite and lipid samples were dried in a speed vac, and
SP:SAMPLEPREP_SUMMARY            	500 µl 2:1 (v:v) cold chloroform:methanol was added to the lipids, which were
SP:SAMPLEPREP_SUMMARY            	ultimately stored along with the dry metabolites at -20 °C until analysis.
SP:SAMPLEPREP_PROTOCOL_FILENAME  	Lipidomics_and_metabolomics.pdf
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_TYPE           	Other
CH:INSTRUMENT_NAME               	Waters Acquity H-Class
CH:COLUMN_NAME                   	Waters ACQUITY UPLC CSH C18 (150 x 3mm, 1.7um)
CH:SOLVENT_A                     	40% acetonitrile/60% water; 10 mM ammonium acetate
CH:SOLVENT_B                     	10% acetonitrile/90% isopropanol; 10 mM ammonium acetate
CH:FLOW_GRADIENT                 	Time (min), %B: 0, 40; 2, 50; 3, 60; 12, 70; 15, 75; 17, 78; 19, 85; 22, 92; 25,
CH:FLOW_GRADIENT                 	99; 34, 99; and 34.5, 40
CH:FLOW_RATE                     	250 uL/min
CH:COLUMN_TEMPERATURE            	42
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
AN:ANALYSIS_PROTOCOL_FILE        	Lipidomics_and_metabolomics.pdf
#MS
MS:INSTRUMENT_NAME               	Thermo Velos Pro Orbitrap
MS:INSTRUMENT_TYPE               	Orbitrap
MS:MS_TYPE                       	ESI
MS:ION_MODE                      	NEGATIVE
MS:MS_COMMENTS                   	Lipid identifications were made using MS-DIAL v4.92 for peak detection,
MS:MS_COMMENTS                   	identification and alignment. The tandem mass spectra and corresponding fragment
MS:MS_COMMENTS                   	ions, mass measurement error, and aligned chromatographic features were manually
MS:MS_COMMENTS                   	examined to remove false positives. Relative quantification was performed by
MS:MS_COMMENTS                   	calculating peak areas on extracted ion chromatograms of precursor masses
#MS_METABOLITE_DATA
MS_METABOLITE_DATA:UNITS	median normalized log 2 transformed peak area
MS_METABOLITE_DATA_START
Samples	Maloyan_Asth_FOH1_l_19	Maloyan_Asth_FOH2_l_20	Maloyan_Asth_FOH3_l_21	Maloyan_Asth_FOH4_l_22	Maloyan_Asth_FOH5_l_23	Maloyan_Asth_FOH6_l_24	Maloyan_Asth_FOR1_l_13	Maloyan_Asth_FOR2_l_14	Maloyan_Asth_FOR3_l_15	Maloyan_Asth_FOR4_l_16	Maloyan_Asth_FOR5_l_17	Maloyan_Asth_FOR6_l_18	Maloyan_Asth_MOH1_l_07	Maloyan_Asth_MOH2_l_08	Maloyan_Asth_MOH3_l_09	Maloyan_Asth_MOH4_l_10	Maloyan_Asth_MOH5_l_11	Maloyan_Asth_MOH6_l_12	Maloyan_Asth_MOR1_l_01	Maloyan_Asth_MOR2_l_02	Maloyan_Asth_MOR3_l_03	Maloyan_Asth_MOR4_l_04	Maloyan_Asth_MOR5_l_05	Maloyan_Asth_MOR6_l_06	
Factors	Offspring group:Offspring of High-Fat-Diet | Sex:Female | Sample source:Bone marrow	Offspring group:Offspring of High-Fat-Diet | Sex:Female | Sample source:Bone marrow	Offspring group:Offspring of High-Fat-Diet | Sex:Female | Sample source:Bone marrow	Offspring group:Offspring of High-Fat-Diet | Sex:Female | Sample source:Bone marrow	Offspring group:Offspring of High-Fat-Diet | Sex:Female | Sample source:Bone marrow	Offspring group:Offspring of High-Fat-Diet | Sex:Female | Sample source:Bone marrow	Offspring group:Offspring of Regular Diet | Sex:Female | Sample source:Bone marrow	Offspring group:Offspring of Regular Diet | Sex:Female | Sample source:Bone marrow	Offspring group:Offspring of Regular Diet | Sex:Female | Sample source:Bone marrow	Offspring group:Offspring of Regular Diet | Sex:Female | Sample source:Bone marrow	Offspring group:Offspring of Regular Diet | Sex:Female | Sample source:Bone marrow	Offspring group:Offspring of Regular Diet | Sex:Female | Sample source:Bone marrow	Offspring group:Offspring of High-Fat-Diet | Sex:Male | Sample source:Bone marrow	Offspring group:Offspring of High-Fat-Diet | Sex:Male | Sample source:Bone marrow	Offspring group:Offspring of High-Fat-Diet | Sex:Male | Sample source:Bone marrow	Offspring group:Offspring of High-Fat-Diet | Sex:Male | Sample source:Bone marrow	Offspring group:Offspring of High-Fat-Diet | Sex:Male | Sample source:Bone marrow	Offspring group:Offspring of High-Fat-Diet | Sex:Male | Sample source:Bone marrow	Offspring group:Offspring of Regular Diet | Sex:Male | Sample source:Bone marrow	Offspring group:Offspring of Regular Diet | Sex:Male | Sample source:Bone marrow	Offspring group:Offspring of Regular Diet | Sex:Male | Sample source:Bone marrow	Offspring group:Offspring of Regular Diet | Sex:Male | Sample source:Bone marrow	Offspring group:Offspring of Regular Diet | Sex:Male | Sample source:Bone marrow	Offspring group:Offspring of Regular Diet | Sex:Male | Sample source:Bone marrow
Cer 42:1;2O|Cer 18:1;2O/24:0	22.257973	22.28803287	22.21142144	22.35407861	22.34026777	22.06144591	21.98646349	22.25422228	22.34879075	21.98134587	22.21716782	22.37863995	21.9178282	21.64115677	22.40351389	22.15085133	22.16077975	22.37652143	22.2614903	22.17122675	22.04665988	21.99546208	22.13821491	22.11279107	
Cer 42:2;2O|Cer 18:1;2O/24:1	23.98623853	23.8785695	23.77662707	24.1119468	24.06818677	23.66138879	23.28936418	23.6691156	23.66292689	23.71531411	23.61317034	23.9018364	23.80628695	23.03176618	24.02382467	23.62389819	23.86313822	24.02785556	23.5858475	23.58393861	23.3577556	23.1847442	23.34118268	23.6061909	
CL 80:9|CL 18:0_18:0_22:3_22:6	23.47222152	23.75044816	23.61429041	23.43119058	23.53919195	23.32542312	23.19585289	23.41346569	23.33308313	22.97724364	23.32565572	23.67300776	23.28324878	22.7415586	23.16884937	23.1087663	23.19704041	22.99465346	23.55951275	22.14038329	22.5904661	22.0511749	22.6301453	23.44138733	
PE 34:1|PE 16:0_18:1	23.37867158	23.3167007	23.47232265	23.41012494	23.43558788	23.31200841	23.1885048	22.97236944	23.04734332	23.07572608	23.31916416	23.19109825	23.26354364	23.25358418	23.23920119	23.28347678	23.34372945	23.22124852	23.20271278	23.22770242	23.42426995	23.46854822	23.17598799	23.20211232	
PE 34:2|PE 16:0_18:2	25.34297842	25.39203248	25.19046508	24.92553458	25.49079376	24.97067301	24.99221496	25.19319776	24.92229007	24.74029836	25.13084783	25.2422045	24.89405279	24.11491803	25.38842526	25.13106862	25.19660582	25.12255918	25.27174047	23.61156362	24.39818277	23.94544359	24.45149985	24.97703337	
PE 36:1|PE 18:0_18:1	24.34437905	24.22364051	24.69829724	24.34914427	24.46303003	24.20041612	24.28723587	24.00726322	24.27704532	24.48308835	24.12054116	24.3443149	24.22280289	23.62154429	24.18629781	24.25640032	24.25002313	24.21102204	24.60995994	24.46024143	24.31496667	24.35961779	24.19730915	24.11578679	
PE 36:2|PE 18:0_18:2	25.01529866	25.01019867	25.10621529	25.1766531	25.1470425	25.01310381	25.18068238	25.15858064	24.92117683	25.04909974	25.15710387	25.02512385	25.35814403	25.21979518	24.94545615	24.82890557	24.97454648	24.83688907	25.22570241	25.44751424	25.02445316	24.92017018	25.08222725	24.98655253	
PE 36:3|PE 18:1_18:2	23.81811438	24.05923746	23.41948633	22.97676209	24.13212852	23.64984407	23.37343844	23.50945983	23.43789213	23.14342335	23.64802066	23.62386143	23.36769631	22.73534203	23.91284974	23.87784733	23.78973459	23.63615501	23.67371395	22.53068361	22.70461646	23.0040385	23.26896164	23.47315284	
PE 36:4|PE 16:0_20:4	23.4038972	23.27361784	23.57857928	23.31232874	23.75077749	23.29176543	22.91648974	23.41890024	22.98243278	23.11736882	22.41623036	23.09146383	22.36063594	22.90904841	23.13958331	23.19528817	23.19373356	23.09236466	23.30138733	22.82938072	23.41058506	23.29009829	22.78916326	22.94329717	
PE 36:4|PE 18:2_18:2	19.94078926	19.8762768	19.64804383	20.04669724	20.3281597	19.95957944	20.37031752	20.22271962	20.10705611	20.12189424	19.93487498	19.94930768	19.89213451	19.81827661	19.99945343	19.94292778	20.0052942	19.9699608	20.17248814	20.35169402	20.28187191	20.4268491	20.39748503	20.23231755	
PE 38:2|PE 18:1_20:1	21.1645785	21.06021699	20.97604832	20.93972433	21.15009734	20.90131605	20.37398416	20.99151339	20.86862537	20.86861193	20.93716749	20.84640521	20.65134145	20.49604374	20.94042007	20.90363329	21.03078137	21.04308932	20.87621252	21.00806531	20.78193796	20.74125732	20.74759671	20.86096909	
PE 38:3|PE 18:0_20:3	22.41626214	22.27284255	22.81541711	22.62063051	22.44997501	22.52646866	22.63736073	22.492837	22.46248143	22.70461646	22.55894156	22.57456392	22.86836114	22.70461646	22.61713157	18.62060802	22.70461646	22.5213261	22.77244309	22.94695988	22.94003619	23.06688863	22.83770669	22.70093706	
PE 38:4|PE 18:0_20:4	25.837129	25.66304533	25.65247767	26.06611219	25.93701424	25.61410716	25.92553988	25.89956857	25.75710649	25.61985011	25.92506249	25.16815183	26.16206176	25.94283984	25.69267037	25.48024153	25.70184152	25.69776966	25.29506963	26.19937542	25.68035352	26.14844508	25.93615861	25.81997003	
PE 38:5|PE 18:1_20:4	25.05693439	25.02798188	25.05774181	24.49748294	25.2027748	24.74021439	24.36741465	24.72394115	24.50330742	24.15103347	24.77239711	24.78155031	24.04483455	23.82257351	25.15026008	24.94393124	25.08609598	24.98468737	24.75815324	23.01237129	23.88004167	23.62111329	23.62740214	24.22330474	
PE 38:6|PE 16:0_22:6	20.79507417	21.10366628	21.05483842	20.40147887	21.42677834	20.81583316	20.85492273	20.78045178	21.05458378	20.65799432	20.92284738	20.91423408	20.02760559	20.71887177	21.12685769	20.97256281	21.30319037	21.10233035	20.71224429	20.28525755	21.13060657	21.25356155	20.79924848	20.99386837	
PE 38:6|PE 18:2_20:4	20.51732362	20.5138238	20.58658695	20.82459076	20.8539396	20.12861224	20.04655312	20.65570748	20.6790107	20.57625122	20.57978136	20.56979311	20.4778117	20.73005055	20.68948773	20.64279751	20.75895456	20.63820915	20.60099998	20.83180625	20.82181025	20.9750594	19.82251785	20.58533333	
PE 40:4|PE 18:0_22:4	22.93649392	22.70461646	22.59871875	22.07082179	22.70461646	22.7516595	22.38656371	22.62045115	22.59445614	22.55982841	22.72675048	22.65424349	22.27006754	22.56180785	22.5866752	22.57781271	22.55646602	22.70461646	22.70423229	22.40834907	22.60313359	22.85306225	22.28616442	22.28619735	
PE 40:5|PE 18:0_22:5	21.78373524	21.8038736	20.93022104	21.49979133	21.73021103	20.83876799	21.76414664	21.78921598	21.81818304	21.38966862	21.82487238	21.26085666	21.41721978	21.57757264	21.17661305	21.19560557	21.31027544	21.58333236	21.49869219	21.53835999	21.12207804	22.48309261	21.76489681	21.68956838	
PE 40:6|PE 18:0_22:6	22.69395535	22.58193309	22.62332258	22.19442247	23.15428958	22.62941968	22.76426514	22.70461646	22.73069312	22.31843081	22.80204909	22.46257478	22.34072589	22.7506578	22.78400882	22.7858792	22.80549839	22.63699373	22.70461646	22.67692138	22.97436014	23.20636456	22.51463239	22.73434489	
PE O-34:0|PE O-18:0_16:0	22.35527453	22.49685802	22.52176223	22.70461646	22.64871147	22.51276089	22.68832869	22.72463049	22.70461646	22.78090658	22.68003052	22.70461646	22.70229901	22.63385279	22.48870865	22.37686058	22.44656674	22.45180672	22.10322986	22.55548069	22.20373164	22.2559021	22.70461646	22.57330946	
PE O-34:1|PE O-16:0_18:1	23.65743613	23.61857051	23.80967891	24.09216833	23.73484403	23.64944743	23.72754797	23.51149713	23.61292578	23.77355581	23.85500063	23.98856241	24.21754476	23.93792119	23.81510147	23.71534944	23.81693937	23.65919637	23.95984141	24.26238915	23.69777569	23.51208608	23.80729077	23.82230454	
PE O-34:1|PE O-18:1_16:0	21.27031622	21.13834302	20.71514455	20.47891434	21.45771599	20.79174531	20.54787573	21.471433	20.8965375	20.25281201	20.38151854	20.58575783	21.26541408	20.75357761	20.97673285	20.87599858	21.24481299	21.3008464	20.49223795	21.01714678	20.83869957	20.81184726	21.07673302	20.53884767	
PE O-34:2|PE O-16:0_18:2	22.71523261	22.79996046	22.70461646	23.07635086	22.97758975	22.80523923	23.23744289	23.11299013	22.95444554	22.88865596	23.05903949	22.99067308	23.54109011	22.88708611	22.96197793	22.93512487	22.85912673	22.69195374	23.1753017	23.55345829	22.84168224	23.06573225	22.76504257	22.78363036	
PE O-34:2|PE O-18:2_16:0	24.31757946	24.02635455	24.13027896	24.50738747	24.15278846	23.93840862	24.0037704	24.25970798	23.83943088	23.79278088	23.80182979	24.21056873	24.90927542	24.39099375	24.20610895	24.04884091	24.35332418	24.23573359	24.12034144	24.90617105	24.31205226	24.30422743	24.34424071	24.07257101	
PE O-34:3|PE O-16:1_18:2	24.18612668	23.99592375	23.99321435	24.21540704	24.32682227	23.91567571	24.24814008	24.53800779	23.87503145	23.52544867	23.84419984	24.18945124	24.99133425	24.27286778	24.06280239	23.9967441	24.19823127	24.03222613	24.52434238	24.91981165	24.43447202	24.60571566	24.49692525	24.30970705	
PE O-36:1|PE O-18:0_18:1	23.61482789	23.77281047	23.78547302	24.0180044	23.89264198	23.66064994	23.71805532	23.70471049	23.67295692	23.60659441	23.69340315	23.95872645	24.21625927	23.92044031	23.73643521	23.48571337	23.71759027	23.59800924	23.17272782	23.39691685	23.70028434	23.32206551	23.79852542	23.74561573	
PE O-36:2|PE O-18:0_18:2	23.66956554	23.09753021	23.19644345	23.57295662	23.29075503	23.11625423	23.2587043	23.19335345	23.02301941	23.12803394	23.39591845	24.53875575	23.60732631	23.2308758	23.26249164	24.24130585	23.16350056	23.92384012	24.55787789	23.75511244	24.22627154	23.23407119	24.48705774	23.02726313	
PE O-36:2|PE O-18:1_18:1	24.2759468	24.2467058	24.1470287	24.48117946	24.4854916	23.9716028	24.00856783	24.48975602	24.07449016	23.82484056	23.83409209	24.21697965	24.92561838	24.35478766	24.26158975	24.04327778	24.25083135	24.27640916	23.95035242	24.84494793	23.91380973	23.55113144	24.03365189	23.87001553	
PE O-36:3|PE O-18:1_18:2_A	22.76434749	22.70623704	22.94712048	23.12286039	22.86150129	22.80425037	23.08820308	22.89739602	22.78960625	22.78249331	23.0009402	23.01516557	23.60180344	22.98133012	23.01269291	22.97537866	22.96248943	22.76488947	23.20237066	23.45106671	22.8122012	22.67776389	23.02230813	22.9700153	
PE O-36:3|PE O-18:1_18:2_B	25.15636485	25.10378108	24.93113186	25.03596226	25.19579873	24.96115174	25.04004715	25.3563962	24.96967695	24.75935976	24.73044823	25.13069473	25.94970235	25.29375905	25.12131061	24.85251733	25.10655828	25.08721964	25.12789596	25.89180977	25.16255202	25.36972098	25.42907526	25.09452394	
PE O-36:4|PE O-16:0_20:4	24.43842566	24.28394547	24.28982511	24.41353341	24.5335496	24.14600211	24.41091314	24.58398296	24.17862754	23.96216857	24.28282854	24.49798213	25.10939706	24.47616065	24.46951338	24.35114089	24.5366083	24.29974001	24.71621578	25.22183127	24.53773318	24.66706619	24.53985417	24.40712502	
PE O-36:5|PE O-16:1_20:4	24.81358882	24.89059803	24.27745326	25.01515934	25.12049065	24.58743047	24.60169502	24.99713997	24.42878857	24.16259442	24.50392534	24.92465629	25.41313549	24.35099771	24.75060505	24.68231072	24.94822367	24.71356809	24.74941891	25.56010286	25.00400009	25.23825168	24.90723128	24.71816995	
PE O-36:5|PE O-18:3_18:2	21.47616685	21.43523024	21.1577485	21.379741	21.58060904	21.08063062	21.09745459	21.77062184	20.85797412	20.42327645	20.83664279	21.12652199	21.89682811	19.79311724	21.3662709	21.1251545	21.28618718	21.22775069	21.8426284	21.4519316	21.07120478	20.54933334	21.2309638	21.03962564	
PE O-38:1|PE O-20:0_18:1	20.70723584	20.45996375	20.93947677	20.95825007	21.13038881	20.77469872	20.93405116	21.00058111	20.8212763	20.75637621	21.01325677	20.9532027	20.93657599	20.68611193	21.10396182	20.81771055	20.98683415	20.80869582	20.44785656	20.59689162	20.42035657	19.88264832	20.79678237	20.86627444	
PE O-38:2|PE O-18:1_20:1	20.36962289	21.04002519	20.89315024	20.99472499	21.25296262	20.8194644	20.80604221	21.1469644	20.57239353	20.39829579	20.61551601	20.93751247	21.3244592	20.70864384	21.09738243	20.80350679	20.97945818	20.93490811	20.4172082	21.11021765	20.92184843	20.44887485	19.98058025	20.61386905	
PE O-38:2|PE O-20:0_18:2	21.14689085	21.09976288	21.14464833	21.36243312	21.45412418	21.18787311	21.57895491	21.43768281	20.69866107	21.19348642	21.10014696	20.90851288	21.74459681	21.37910061	21.18165696	21.11523119	20.91915701	20.75681861	20.61991862	21.11454084	21.17686242	20.89504552	21.48563965	21.46970032	
PE O-38:3|PE O-18:0_20:3	20.48550383	20.5862641	20.54026614	20.14171998	20.56785791	20.10564879	20.92771407	20.74633397	20.87502792	20.45466455	20.87763386	20.9231762	20.49367698	20.33509969	20.7837976	20.72195287	20.77789317	20.74327337	20.59490338	20.6581921	21.08328363	20.76438192	20.94251247	21.27594184	
PE O-38:3|PE O-20:1_18:2	22.16927769	22.06967548	21.70315693	22.24263459	22.27221623	21.73332208	21.95485827	22.28599515	21.85794773	21.50889319	21.45553634	21.94185069	22.70461646	22.00070097	21.98148303	21.63982855	22.07515475	21.97697806	21.5082275	21.78768417	21.85382149	21.64866634	22.12476808	21.89790745	
PE O-38:4|PE O-18:1_20:3	24.21513486	24.21908609	24.23967487	24.49388947	24.2742455	24.0842466	24.21707887	24.32988023	24.20436428	24.16972645	24.15689387	24.40617358	24.84504104	24.40508999	24.26662303	24.11415332	24.24439488	24.08028475	24.11407646	24.58774611	24.58119167	24.71696277	24.54803948	24.43829761	
PE O-38:5|PE O-18:1_20:4_B	25.39765837	25.35213786	25.37013145	25.87710716	25.51297645	25.1807897	25.38640278	25.54174427	25.09318608	25.18422411	25.03248585	25.39173416	25.93130058	25.74298401	25.3343112	24.99839282	25.35131694	25.25677872	25.35165067	26.1406895	25.4687514	25.53142964	25.49279294	25.13117886	
PE O-38:5|PE O-18:1_20:4_A	23.2182607	22.99753438	23.18000551	23.04478343	23.28804154	22.96266829	23.2686527	23.22204341	23.08407408	22.72817975	23.26131968	23.27838758	23.60035276	23.15717439	23.23112457	23.15481263	23.23455562	23.01351844	23.44287237	23.67065196	23.13456387	23.15665763	23.13795294	23.14976678	
PE O-38:6|PE O-18:2_20:4	24.17952307	24.09020785	23.88311412	24.23428303	24.36227174	23.40021349	23.85994881	23.81692821	23.63441088	23.41988506	23.72084646	24.01276357	24.64824082	24.13322622	24.06106243	24.02114892	24.38062768	23.71420145	24.28332155	24.74770166	24.25273115	24.26480503	24.08374738	23.98465106	
PE O-38:7|PE O-16:1_22:6	22.47561277	22.66961797	22.09253123	22.25931791	22.56896754	22.22107836	22.01929668	22.66250897	22.00605672	21.81729599	21.67090449	22.21345519	22.50148017	22.23674574	22.47534233	22.30417444	22.5934181	22.48071001	22.62568927	22.99596912	22.47175263	22.6315375	22.48465052	22.27944622	
PE O-42:6|PE O-22:2_20:4	21.22610481	21.27787617	21.03985843	21.29235719	21.45433993	20.82310311	20.9295429	21.3822729	20.99935903	20.70330056	20.78449443	21.136759	21.73766771	21.18975045	21.1181215	21.0660172	21.23878033	21.27225067	20.46935233	21.3019469	20.78342393	20.42308561	20.98625511	20.72782566	
PG 34:1|PG 16:0_18:1	22.62180307	22.73802771	22.45894475	22.4602228	22.61806544	22.45691678	22.35639793	22.53702787	22.51908057	22.10314296	22.60036023	22.46740629	22.49633214	22.30004288	22.70461646	22.73835128	22.25687959	22.4389806	22.09282896	22.51869455	22.37143711	22.3941614	22.37387982	22.44414068	
PG 36:2|PG 18:1_18:1	20.98770439	20.96595488	20.62479234	20.57915534	21.07899825	20.75257232	20.02807204	20.27159423	19.97701523	19.72092452	20.69802642	20.60846624	20.01583312	19.79037899	21.09229933	20.36164761	20.53598521	20.85388091	19.31239795	19.59659804	19.40359321	19.28585864	19.44762039	19.63861799	
PS 32:0	19.36871114	19.69753205	19.80824588	19.82391954	19.57237684	19.60699258	19.39007163	19.32135018	19.18737894	19.54920951	19.68055438	19.88228575	19.66368446	19.19126548	19.53359177	19.10284969	19.46659515	19.58484719	19.2702865	19.18389409	19.33685206	19.33511326	19.30539969	19.32230674	
PS 34:1	22.70461646	22.55725383	22.88358292	23.12044465	22.67704467	22.70461646	22.70461646	22.52165316	22.58379972	22.66747651	22.70461646	22.89076386	22.85358271	22.69620092	22.38398741	22.54387593	22.65221317	22.74156908	22.51257452	23.30628272	22.85912346	22.70461646	22.76934721	22.70461646	
PS 36:1|PS 18:0_18:1	23.25747323	22.21660429	22.3446234	22.00485344	22.65903655	22.57071224	22.23480971	22.46226771	22.57271102	21.82189427	22.26034244	22.19791305	21.84630396	21.42832513	22.25745504	22.70461646	22.48075721	23.01705359	22.18978546	21.02755961	21.50027215	21.05520846	22.07891341	22.17742541	
PS 36:2|PS 18:0_18:2	25.40730301	25.38224219	25.70775952	26.0887878	25.20955431	25.4898614	25.45879635	25.48071526	25.57380674	25.68330421	25.21654861	25.68006491	26.08921165	24.88596112	25.35357197	24.91778389	25.03961872	25.26450547	25.31822391	25.94648913	24.99887133	25.70902358	25.44102621	25.43669889	
PS 36:3	21.15313749	21.55543894	21.33510068	21.70791404	21.6067527	20.89451184	21.34099063	20.74355734	21.36874871	21.27605299	21.57649462	21.68570711	21.69430937	21.35258972	21.49286147	21.31568836	21.61103418	21.2537539	21.50625196	21.27495499	21.12377388	20.88169562	21.1536718	20.98922294	
PS 38:3	23.18701312	22.76863027	23.72088892	24.05387115	23.55822884	23.20002969	23.36792405	23.38740029	23.66720351	23.93344943	23.56099173	23.81692071	24.16193181	23.14978775	23.68575641	23.51129522	23.69916774	23.49123677	24.19969794	23.88515598	23.92774439	23.49622706	23.79450575	23.70378472	
PS 38:5	19.30756008	19.6965027	18.94195951	19.66806244	19.77158469	19.21142521	18.99067333	19.2636085	18.87627682	18.99115947	19.39445962	19.53708674	19.0503986	19.23243326	19.52918069	19.30390013	19.75959196	19.55872898	19.35252788	19.10266264	19.29466318	19.08364605	18.80797301	18.93353369	
PS 40:5_A	21.34022197	21.43797281	21.56681098	21.64791324	21.58485643	21.30087875	21.22615322	21.24408196	21.16889819	21.2243595	21.39448827	21.61919283	21.37468607	21.12740758	21.60256274	21.01368785	21.01482782	20.99790119	21.20698699	21.54361998	21.43885618	21.24604424	21.42906547	21.10346179	
PS 40:5_B	21.14821471	21.59171877	21.5495064	21.49407326	21.62222022	21.38600059	21.3572781	21.35158426	22.04537616	21.51312265	21.22677113	21.17112465	21.09264059	20.99680406	21.0501147	21.28661317	21.31834372	21.0442846	21.24693959	21.58185729	21.47932728	21.89821892	20.9135373	21.24867596	
PS 40:6	22.60451471	23.01999686	23.11010792	23.28854882	23.14887648	22.99780816	23.00119412	22.9107124	22.91494344	23.01796179	22.93721039	23.00612014	22.98511464	23.04563202	23.01895741	22.64653839	22.9457022	23.051811	22.88001202	22.99362012	23.20912422	23.18372138	22.89543247	23.01455091	
SM 33:1;2O|SM 17:0;2O/16:1	20.3647607	20.2018503	20.59495993	20.59436497	20.52417739	20.14834224	19.55257337	20.0667594	20.07178138	20.11129992	20.17749867	20.3876197	19.77911182	19.72123473	19.87964996	19.45168526	19.65975794	19.32265575	19.71426589	19.97045959	19.11227884	19.15314455	19.32774025	19.0387205	
SM 41:1;2O|SM 25:0;2O/16:1	22.22304225	22.1470337	22.65246581	21.85349303	22.1270557	21.9739654	21.87121337	22.14018164	22.44207155	22.17891924	22.24772789	21.64111987	22.05661303	21.84335499	22.24595127	21.74250351	22.10074479	22.10394945	22.18574126	22.70461646	21.70934047	21.48755903	21.81839624	22.02079034	
SM 41:2;2O|SM 14:2;2O/27:0	21.26816832	21.28800547	21.59804338	20.77927363	21.51334919	20.90971814	21.26023192	21.21119755	21.42282662	20.71445592	21.34966892	21.43112759	20.70311566	20.4935799	21.26102345	21.0271485	21.24155921	21.06983878	20.60319889	21.09965096	20.72068811	20.50346147	20.67024386	21.04285514	
MS_METABOLITE_DATA_END
#METABOLITES
METABOLITES_START
metabolite_name	KEGG	PUBCHEM
Cer 42:1;2O|Cer 18:1;2O/24:0	-	154573074 
Cer 42:2;2O|Cer 18:1;2O/24:1	-	154573075 
CL 80:9|CL 18:0_18:0_22:3_22:6	-	449005  
PE 34:1|PE 16:0_18:1	-	5283496 
PE 34:2|PE 16:0_18:2	-	9546747  
PE 36:1|PE 18:0_18:1	-	 9546734 
PE 36:2|PE 18:0_18:2	-	6437392
PE 36:3|PE 18:1_18:2	-	52924364
PE 36:4|PE 16:0_20:4	-	52924875
PE 36:4|PE 18:2_18:2	-	52924875
PE 38:2|PE 18:1_20:1	-	9546825
PE 38:3|PE 18:0_20:3	-	52924240 
PE 38:4|PE 18:0_20:4	-	5289133 
PE 38:5|PE 18:1_20:4	-	52925089 
PE 38:6|PE 16:0_22:6	-	52924893
PE 38:6|PE 18:2_20:4	-	52924893
PE 40:4|PE 18:0_22:4	-	52924901  
PE 40:5|PE 18:0_22:5	-	53479611 
PE 40:6|PE 18:0_22:6	-	9546798  
PE O-34:0|PE O-18:0_16:0	-	85332378  
PE O-34:1|PE O-16:0_18:1	-	 160713692 
PE O-34:1|PE O-18:1_16:0	-	 160713692 
PE O-34:2|PE O-16:0_18:2	-	6443157  
PE O-34:2|PE O-18:2_16:0	-	6443157  
PE O-34:3|PE O-16:1_18:2	-	160713694  
PE O-36:1|PE O-18:0_18:1	-	160713699  
PE O-36:2|PE O-18:0_18:2	-	 160713700  
PE O-36:2|PE O-18:1_18:1	-	 160713700  
PE O-36:3|PE O-18:1_18:2_A	-	 160713701 
PE O-36:3|PE O-18:1_18:2_B	-	 160713701 
PE O-36:4|PE O-16:0_20:4	-	160713702 
PE O-36:5|PE O-16:1_20:4	-	160713703  
PE O-36:5|PE O-18:3_18:2	-	160713703  
PE O-38:1|PE O-20:0_18:1	-	160713706 
PE O-38:2|PE O-18:1_20:1	-	160713707 
PE O-38:2|PE O-20:0_18:2	-	160713707 
PE O-38:3|PE O-18:0_20:3	-	 160713708 
PE O-38:3|PE O-20:1_18:2	-	 160713708 
PE O-38:4|PE O-18:1_20:3	-	160713709 
PE O-38:5|PE O-18:1_20:4_B	-	160713710  
PE O-38:5|PE O-18:1_20:4_A	-	160713710  
PE O-38:6|PE O-18:2_20:4	-	160713711  
PE O-38:7|PE O-16:1_22:6	-	85735795 
PE O-42:6|PE O-22:2_20:4	-	160713640 
PG 34:1|PG 16:0_18:1	-	52926330  
PG 36:2|PG 18:1_18:1	-	52926417  
PS 32:0	-	9547090 
PS 34:1	-	52926066
PS 36:1|PS 18:0_18:1	-	9547087 
PS 36:2|PS 18:0_18:2	-	9547091  
PS 36:3	-	52926065  
PS 38:3	-	52925334 
PS 38:5	-	52925988  
PS 40:5_A	-	53480355 
PS 40:5_B	-	53480355 
PS 40:6	-	24779546 
SM 33:1;2O|SM 17:0;2O/16:1	-	70679243 
SM 41:1;2O|SM 25:0;2O/16:1	-	154573108  
SM 41:2;2O|SM 14:2;2O/27:0	-	52931207  
METABOLITES_END
#END