#METABOLOMICS WORKBENCH SongJiawei_20241022_082845 DATATRACK_ID:5301 STUDY_ID:ST003534 ANALYSIS_ID:AN005805 PROJECT_ID:PR002168
VERSION             	1
CREATED_ON             	October 24, 2024, 8:40 pm
#PROJECT
PR:PROJECT_TITLE                 	Arachidonic acid fuels inflammation by unlocking 5-LOX in macrophages after
PR:PROJECT_TITLE                 	myocardial infarction
PR:PROJECT_SUMMARY               	An overactive inflammatory response and immune cell infiltration in myocardial
PR:PROJECT_SUMMARY               	infarction (MI) impairs tissue repair. Arachidonic acid (AA) metabolites act as
PR:PROJECT_SUMMARY               	important sources of inflammatory mediators in cardiac tissue. We investigated
PR:PROJECT_SUMMARY               	the mechanisms underlying the AA cascade-mediated inflammatory response after
PR:PROJECT_SUMMARY               	MI. Metabolomics analyses revealed that the 5-lipoxygenase (5-LOX)-dependent AA
PR:PROJECT_SUMMARY               	metabolic pathway derivative leukotriene B4 (LTB4) accumulated in large
PR:PROJECT_SUMMARY               	quantities after MI. Elevated levels of AA in vivo after MI facilitated the
PR:PROJECT_SUMMARY               	recruitment of protein phosphatase 5 (PP5) into the nucleus by binding PP5 to
PR:PROJECT_SUMMARY               	its nuclear transporter receptor karyopherin subunit alpha 1. On entering the
PR:PROJECT_SUMMARY               	nucleus, PP5 directly dephosphorylated 5-LOX at Thr218, promoting the production
PR:PROJECT_SUMMARY               	of the proinflammatory mediator LTB4 by macrophages. Transgenic mice were
PR:PROJECT_SUMMARY               	created with a population of cardiac resident macrophages with a mutation at
PR:PROJECT_SUMMARY               	5-LOX Thr218. Single-cell transcriptomics showed that these cells overexpressed
PR:PROJECT_SUMMARY               	CXC-chemokine ligand 13 and actively recruited B cells to the heart,
PR:PROJECT_SUMMARY               	exacerbating tissue inflammation. In a UK Biobank cohort, the PP5 E76D mutation
PR:PROJECT_SUMMARY               	was found to be associated with complications after acute MI. Overall, our
PR:PROJECT_SUMMARY               	results have elucidated a conserved role for 5-LOX-phosphorylation-regulated
PR:PROJECT_SUMMARY               	LTB4 levels in MI and identified PP5 as a potential therapeutic target for the
PR:PROJECT_SUMMARY               	treatment of MI.
PR:INSTITUTE                     	Tianjin Medical University
PR:LAST_NAME                     	Song
PR:FIRST_NAME                    	Jiawei
PR:ADDRESS                       	Tianjin Medical University, Tianjin, China., Tianjin, Tianjin, 300070, China
PR:EMAIL                         	sjw808@tmu.edu.cn
PR:PHONE                         	15309216256
#STUDY
ST:STUDY_TITLE                   	Metabolomic analysis of altered arachidonic acid metabolism in mouse heart
ST:STUDY_TITLE                   	tissue after myocardial infarction
ST:STUDY_SUMMARY                 	We performed myocardial infarction surgery on wild-type (WT) mice and collected
ST:STUDY_SUMMARY                 	cardiac tissues from the sham group and 1, 7, 14 and 28 days after surgery for
ST:STUDY_SUMMARY                 	LC-MS analysis. The results showed that AA-derived LTB4 was produced in large
ST:STUDY_SUMMARY                 	quantities 2 weeks after myocardial infarction and remained at a high level for
ST:STUDY_SUMMARY                 	a long time.
ST:INSTITUTE                     	Tianjin Medical University
ST:LAST_NAME                     	Song
ST:FIRST_NAME                    	Jiawei
ST:ADDRESS                       	Tianjin Medical University, Tianjin, China., Tianjin, Tianjin, 300070, China
ST:EMAIL                         	sjw808@tmu.edu.cn
ST:PHONE                         	15309216256
#SUBJECT
SU:SUBJECT_TYPE                  	Mammal
SU:SUBJECT_SPECIES               	Mus musculus
SU:TAXONOMY_ID                   	10090
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data
SUBJECT_SAMPLE_FACTORS           	-	MI0d_01	treatment:post-MI day0 | Sample source:heart	RAW_FILE_NAME(raw data file names)=Data_Mice_MI0d_01
SUBJECT_SAMPLE_FACTORS           	-	MI0d_02	treatment:post-MI day0 | Sample source:heart	RAW_FILE_NAME(raw data file names)=Data_Mice_MI0d_02
SUBJECT_SAMPLE_FACTORS           	-	MI0d_03	treatment:post-MI day0 | Sample source:heart	RAW_FILE_NAME(raw data file names)=Data_Mice_MI0d_03
SUBJECT_SAMPLE_FACTORS           	-	MI0d_04	treatment:post-MI day0 | Sample source:heart	RAW_FILE_NAME(raw data file names)=Data_Mice_MI0d_04
SUBJECT_SAMPLE_FACTORS           	-	MI0d_05	treatment:post-MI day0 | Sample source:heart	RAW_FILE_NAME(raw data file names)=Data_Mice_MI0d_05
SUBJECT_SAMPLE_FACTORS           	-	MI0d_06	treatment:post-MI day0 | Sample source:heart	RAW_FILE_NAME(raw data file names)=Data_Mice_MI0d_06
SUBJECT_SAMPLE_FACTORS           	-	MI0d_07	treatment:post-MI day0 | Sample source:heart	RAW_FILE_NAME(raw data file names)=Data_Mice_MI0d_07
SUBJECT_SAMPLE_FACTORS           	-	MI1d_01	treatment:post-MI day1 | Sample source:heart	RAW_FILE_NAME(raw data file names)=Data_Mice_MI1d_01
SUBJECT_SAMPLE_FACTORS           	-	MI1d_02	treatment:post-MI day1 | Sample source:heart	RAW_FILE_NAME(raw data file names)=Data_Mice_MI1d_02
SUBJECT_SAMPLE_FACTORS           	-	MI1d_03	treatment:post-MI day1 | Sample source:heart	RAW_FILE_NAME(raw data file names)=Data_Mice_MI1d_03
SUBJECT_SAMPLE_FACTORS           	-	MI1d_04	treatment:post-MI day1 | Sample source:heart	RAW_FILE_NAME(raw data file names)=Data_Mice_MI1d_04
SUBJECT_SAMPLE_FACTORS           	-	MI1d_05	treatment:post-MI day1 | Sample source:heart	RAW_FILE_NAME(raw data file names)=Data_Mice_MI1d_05
SUBJECT_SAMPLE_FACTORS           	-	MI1d_06	treatment:post-MI day1 | Sample source:heart	RAW_FILE_NAME(raw data file names)=Data_Mice_MI1d_06
SUBJECT_SAMPLE_FACTORS           	-	MI1d_07	treatment:post-MI day1 | Sample source:heart	RAW_FILE_NAME(raw data file names)=Data_Mice_MI1d_07
SUBJECT_SAMPLE_FACTORS           	-	MI7d_01	treatment:post-MI day7 | Sample source:heart	RAW_FILE_NAME(raw data file names)=Data_Mice_MI7d_01
SUBJECT_SAMPLE_FACTORS           	-	MI7d_02	treatment:post-MI day7 | Sample source:heart	RAW_FILE_NAME(raw data file names)=Data_Mice_MI7d_02
SUBJECT_SAMPLE_FACTORS           	-	MI7d_03	treatment:post-MI day7 | Sample source:heart	RAW_FILE_NAME(raw data file names)=Data_Mice_MI7d_03
SUBJECT_SAMPLE_FACTORS           	-	MI7d_04	treatment:post-MI day7 | Sample source:heart	RAW_FILE_NAME(raw data file names)=Data_Mice_MI7d_04
SUBJECT_SAMPLE_FACTORS           	-	MI7d_05	treatment:post-MI day7 | Sample source:heart	RAW_FILE_NAME(raw data file names)=Data_Mice_MI7d_05
SUBJECT_SAMPLE_FACTORS           	-	MI7d_06	treatment:post-MI day7 | Sample source:heart	RAW_FILE_NAME(raw data file names)=Data_Mice_MI7d_06
SUBJECT_SAMPLE_FACTORS           	-	MI7d_07	treatment:post-MI day7 | Sample source:heart	RAW_FILE_NAME(raw data file names)=Data_Mice_MI7d_07
SUBJECT_SAMPLE_FACTORS           	-	MI14d_01	treatment:post-MI day14 | Sample source:heart	RAW_FILE_NAME(raw data file names)=Data_Mice_MI14d_01
SUBJECT_SAMPLE_FACTORS           	-	MI14d_02	treatment:post-MI day14 | Sample source:heart	RAW_FILE_NAME(raw data file names)=Data_Mice_MI14d_02
SUBJECT_SAMPLE_FACTORS           	-	MI14d_03	treatment:post-MI day14 | Sample source:heart	RAW_FILE_NAME(raw data file names)=Data_Mice_MI14d_03
SUBJECT_SAMPLE_FACTORS           	-	MI14d_04	treatment:post-MI day14 | Sample source:heart	RAW_FILE_NAME(raw data file names)=Data_Mice_MI14d_04
SUBJECT_SAMPLE_FACTORS           	-	MI14d_05	treatment:post-MI day14 | Sample source:heart	RAW_FILE_NAME(raw data file names)=Data_Mice_MI14d_05
SUBJECT_SAMPLE_FACTORS           	-	MI14d_06	treatment:post-MI day14 | Sample source:heart	RAW_FILE_NAME(raw data file names)=Data_Mice_MI14d_06
SUBJECT_SAMPLE_FACTORS           	-	MI14d_07	treatment:post-MI day14 | Sample source:heart	RAW_FILE_NAME(raw data file names)=Data_Mice_MI14d_07
SUBJECT_SAMPLE_FACTORS           	-	MI28d_01	treatment:post-MI day28 | Sample source:heart	RAW_FILE_NAME(raw data file names)=Data_Mice_MI28d_01
SUBJECT_SAMPLE_FACTORS           	-	MI28d_02	treatment:post-MI day28 | Sample source:heart	RAW_FILE_NAME(raw data file names)=Data_Mice_MI28d_02
SUBJECT_SAMPLE_FACTORS           	-	MI28d_03	treatment:post-MI day28 | Sample source:heart	RAW_FILE_NAME(raw data file names)=Data_Mice_MI28d_03
SUBJECT_SAMPLE_FACTORS           	-	MI28d_04	treatment:post-MI day28 | Sample source:heart	RAW_FILE_NAME(raw data file names)=Data_Mice_MI28d_04
SUBJECT_SAMPLE_FACTORS           	-	MI28d_05	treatment:post-MI day28 | Sample source:heart	RAW_FILE_NAME(raw data file names)=Data_Mice_MI28d_05
SUBJECT_SAMPLE_FACTORS           	-	MI28d_06	treatment:post-MI day28 | Sample source:heart	RAW_FILE_NAME(raw data file names)=Data_Mice_MI28d_06
SUBJECT_SAMPLE_FACTORS           	-	MI28d_07	treatment:post-MI day28 | Sample source:heart	RAW_FILE_NAME(raw data file names)=Data_Mice_MI28d_07
SUBJECT_SAMPLE_FACTORS           	-	MI0d_01_arachidonic acid	treatment:post-MI day0 | Sample source:heart	RAW_FILE_NAME(raw data file names)=Data_arachidonic acid_Mice_MI0d_01
SUBJECT_SAMPLE_FACTORS           	-	MI0d_02_arachidonic acid	treatment:post-MI day0 | Sample source:heart	RAW_FILE_NAME(raw data file names)=Data_arachidonic acid_Mice_MI0d_02
SUBJECT_SAMPLE_FACTORS           	-	MI0d_03_arachidonic acid	treatment:post-MI day0 | Sample source:heart	RAW_FILE_NAME(raw data file names)=Data_arachidonic acid_Mice_MI0d_03
SUBJECT_SAMPLE_FACTORS           	-	MI0d_04_arachidonic acid	treatment:post-MI day0 | Sample source:heart	RAW_FILE_NAME(raw data file names)=Data_arachidonic acid_Mice_MI0d_04
SUBJECT_SAMPLE_FACTORS           	-	MI0d_05_arachidonic acid	treatment:post-MI day0 | Sample source:heart	RAW_FILE_NAME(raw data file names)=Data_arachidonic acid_Mice_MI0d_05
SUBJECT_SAMPLE_FACTORS           	-	MI0d_06_arachidonic acid	treatment:post-MI day0 | Sample source:heart	RAW_FILE_NAME(raw data file names)=Data_arachidonic acid_Mice_MI0d_06
SUBJECT_SAMPLE_FACTORS           	-	MI0d_07_arachidonic acid	treatment:post-MI day0 | Sample source:heart	RAW_FILE_NAME(raw data file names)=Data_arachidonic acid_Mice_MI0d_07
SUBJECT_SAMPLE_FACTORS           	-	MI1d_01_arachidonic acid	treatment:post-MI day1 | Sample source:heart	RAW_FILE_NAME(raw data file names)=Data_arachidonic acid_Mice_MI1d_01
SUBJECT_SAMPLE_FACTORS           	-	MI1d_02_arachidonic acid	treatment:post-MI day1 | Sample source:heart	RAW_FILE_NAME(raw data file names)=Data_arachidonic acid_Mice_MI1d_02
SUBJECT_SAMPLE_FACTORS           	-	MI1d_03_arachidonic acid	treatment:post-MI day1 | Sample source:heart	RAW_FILE_NAME(raw data file names)=Data_arachidonic acid_Mice_MI1d_03
SUBJECT_SAMPLE_FACTORS           	-	MI1d_04_arachidonic acid	treatment:post-MI day1 | Sample source:heart	RAW_FILE_NAME(raw data file names)=Data_arachidonic acid_Mice_MI1d_04
SUBJECT_SAMPLE_FACTORS           	-	MI1d_05_arachidonic acid	treatment:post-MI day1 | Sample source:heart	RAW_FILE_NAME(raw data file names)=Data_arachidonic acid_Mice_MI1d_05
SUBJECT_SAMPLE_FACTORS           	-	MI1d_06_arachidonic acid	treatment:post-MI day1 | Sample source:heart	RAW_FILE_NAME(raw data file names)=Data_arachidonic acid_Mice_MI1d_06
SUBJECT_SAMPLE_FACTORS           	-	MI1d_07_arachidonic acid	treatment:post-MI day1 | Sample source:heart	RAW_FILE_NAME(raw data file names)=Data_arachidonic acid_Mice_MI1d_07
SUBJECT_SAMPLE_FACTORS           	-	MI7d_01_arachidonic acid	treatment:post-MI day7 | Sample source:heart	RAW_FILE_NAME(raw data file names)=Data_arachidonic acid_Mice_MI7d_01
SUBJECT_SAMPLE_FACTORS           	-	MI7d_02_arachidonic acid	treatment:post-MI day7 | Sample source:heart	RAW_FILE_NAME(raw data file names)=Data_arachidonic acid_Mice_MI7d_02
SUBJECT_SAMPLE_FACTORS           	-	MI7d_03_arachidonic acid	treatment:post-MI day7 | Sample source:heart	RAW_FILE_NAME(raw data file names)=Data_arachidonic acid_Mice_MI7d_03
SUBJECT_SAMPLE_FACTORS           	-	MI7d_04_arachidonic acid	treatment:post-MI day7 | Sample source:heart	RAW_FILE_NAME(raw data file names)=Data_arachidonic acid_Mice_MI7d_04
SUBJECT_SAMPLE_FACTORS           	-	MI7d_05_arachidonic acid	treatment:post-MI day7 | Sample source:heart	RAW_FILE_NAME(raw data file names)=Data_arachidonic acid_Mice_MI7d_05
SUBJECT_SAMPLE_FACTORS           	-	MI7d_06_arachidonic acid	treatment:post-MI day7 | Sample source:heart	RAW_FILE_NAME(raw data file names)=Data_arachidonic acid_Mice_MI7d_06
SUBJECT_SAMPLE_FACTORS           	-	MI7d_07_arachidonic acid	treatment:post-MI day7 | Sample source:heart	RAW_FILE_NAME(raw data file names)=Data_arachidonic acid_Mice_MI7d_07
SUBJECT_SAMPLE_FACTORS           	-	MI14d_01_arachidonic acid	treatment:post-MI day14 | Sample source:heart	RAW_FILE_NAME(raw data file names)=Data_arachidonic acid_Mice_MI14d_01
SUBJECT_SAMPLE_FACTORS           	-	MI14d_02_arachidonic acid	treatment:post-MI day14 | Sample source:heart	RAW_FILE_NAME(raw data file names)=Data_arachidonic acid_Mice_MI14d_02
SUBJECT_SAMPLE_FACTORS           	-	MI14d_03_arachidonic acid	treatment:post-MI day14 | Sample source:heart	RAW_FILE_NAME(raw data file names)=Data_arachidonic acid_Mice_MI14d_03
SUBJECT_SAMPLE_FACTORS           	-	MI14d_04_arachidonic acid	treatment:post-MI day14 | Sample source:heart	RAW_FILE_NAME(raw data file names)=Data_arachidonic acid_Mice_MI14d_04
SUBJECT_SAMPLE_FACTORS           	-	MI14d_05_arachidonic acid	treatment:post-MI day14 | Sample source:heart	RAW_FILE_NAME(raw data file names)=Data_arachidonic acid_Mice_MI14d_05
SUBJECT_SAMPLE_FACTORS           	-	MI14d_06_arachidonic acid	treatment:post-MI day14 | Sample source:heart	RAW_FILE_NAME(raw data file names)=Data_arachidonic acid_Mice_MI14d_06
SUBJECT_SAMPLE_FACTORS           	-	MI14d_07_arachidonic acid	treatment:post-MI day14 | Sample source:heart	RAW_FILE_NAME(raw data file names)=Data_arachidonic acid_Mice_MI14d_07
SUBJECT_SAMPLE_FACTORS           	-	MI28d_01_arachidonic acid	treatment:post-MI day28 | Sample source:heart	RAW_FILE_NAME(raw data file names)=Data_arachidonic acid_Mice_MI28d_01
SUBJECT_SAMPLE_FACTORS           	-	MI28d_02_arachidonic acid	treatment:post-MI day28 | Sample source:heart	RAW_FILE_NAME(raw data file names)=Data_arachidonic acid_Mice_MI28d_02
SUBJECT_SAMPLE_FACTORS           	-	MI28d_03_arachidonic acid	treatment:post-MI day28 | Sample source:heart	RAW_FILE_NAME(raw data file names)=Data_arachidonic acid_Mice_MI28d_03
SUBJECT_SAMPLE_FACTORS           	-	MI28d_04_arachidonic acid	treatment:post-MI day28 | Sample source:heart	RAW_FILE_NAME(raw data file names)=Data_arachidonic acid_Mice_MI28d_04
SUBJECT_SAMPLE_FACTORS           	-	MI28d_05_arachidonic acid	treatment:post-MI day28 | Sample source:heart	RAW_FILE_NAME(raw data file names)=Data_arachidonic acid_Mice_MI28d_05
SUBJECT_SAMPLE_FACTORS           	-	MI28d_06_arachidonic acid	treatment:post-MI day28 | Sample source:heart	RAW_FILE_NAME(raw data file names)=Data_arachidonic acid_Mice_MI28d_06
SUBJECT_SAMPLE_FACTORS           	-	MI28d_07_arachidonic acid	treatment:post-MI day28 | Sample source:heart	RAW_FILE_NAME(raw data file names)=Data_arachidonic acid_Mice_MI28d_07
#COLLECTION
CO:COLLECTION_SUMMARY            	Myocardial infarction (MI) was induced in 8-week-old male or female C57BL/6J
CO:COLLECTION_SUMMARY            	mice by permanent left anterior descending coronary artery ligation. Mice were
CO:COLLECTION_SUMMARY            	then killed by intraperitoneal injection of avertin (30 μl g-1), and cardiac
CO:COLLECTION_SUMMARY            	tissue was isolated by saline perfusion through the left ventricle after removal
CO:COLLECTION_SUMMARY            	of the right auricle.
CO:SAMPLE_TYPE                   	Heart
#TREATMENT
TR:TREATMENT_SUMMARY             	Myocardial infarction (MI) was induced in 8-week-old male or female C57BL/6J
TR:TREATMENT_SUMMARY             	mice by permanent left anterior descending coronary artery ligation. Mice were
TR:TREATMENT_SUMMARY             	then killed by intraperitoneal injection of avertin (30 μl g-1) at 1, 7, 14,
TR:TREATMENT_SUMMARY             	and 28 days after infarction, respectively. Cardiac tissue was isolated by
TR:TREATMENT_SUMMARY             	saline perfusion of the left ventricle after excision of the right auricle.
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	A 50-mg sample of mouse heart tissue was extracted using a centrifuge tube with
SP:SAMPLEPREP_SUMMARY            	low adsorption properties after adding 1 µl of internal standard mixture.
SP:SAMPLEPREP_SUMMARY            	Arachidonic acid (AA) metabolites were extracted with methanol and ethyl acetate
SP:SAMPLEPREP_SUMMARY            	under low light conditions. The extracted samples were evaporated to dryness in
SP:SAMPLEPREP_SUMMARY            	a gentle stream of nitrogen, after which the samples were dissolved in 30%
SP:SAMPLEPREP_SUMMARY            	acetonitrile.
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_SUMMARY        	Chromatographic separation was performed on an UPLC BEH C18 column (1.7 µm, 50
CH:CHROMATOGRAPHY_SUMMARY        	× 2.1 mm i.d).
CH:CHROMATOGRAPHY_TYPE           	Reversed phase
CH:INSTRUMENT_NAME               	Waters Acquity
CH:COLUMN_NAME                   	Waters ACQUITY UPLC BEH C18 (50 x 2.1mm,1.7um)
CH:SOLVENT_A                     	100% water
CH:SOLVENT_B                     	100% acetonitrile
CH:FLOW_GRADIENT                 	0-1min, 25% B; 1-8min, 25% B-95% B; 8-8.5min, 95% B; 8.5-8.51min, 95% B-25% B;
CH:FLOW_GRADIENT                 	8.51-9min, 25%B
CH:FLOW_RATE                     	0.6mL/min
CH:COLUMN_TEMPERATURE            	40
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
#MS
MS:INSTRUMENT_NAME               	Waters Xevo-TQ-S
MS:INSTRUMENT_TYPE               	Other
MS:MS_TYPE                       	ESI
MS:ION_MODE                      	NEGATIVE
MS:MS_COMMENTS                   	AA metabolites were analyzed using a QTRAP 5500 Hybrid Triple Quadrupole Linear
MS:MS_COMMENTS                   	Ion Trap Mass Spectrometer equipped with a Turbo Ion Spray Electrospray Ion
MS:MS_COMMENTS                   	Source. Raw data were processed using MassLynxV4.2. Metabolite levels were
MS:MS_COMMENTS                   	calculated as the ratio of their concentrations relative to a standard sample.
MS:MS_COMMENTS                   	Quantifying the targeted metabolites by area under the curve used expected
MS:MS_COMMENTS                   	retention time.
#MS_METABOLITE_DATA
MS_METABOLITE_DATA:UNITS	ng/mL
MS_METABOLITE_DATA_START
Samples	MI0d_01	MI0d_02	MI0d_03	MI0d_04	MI0d_05	MI0d_06	MI0d_07	MI1d_01	MI1d_02	MI1d_03	MI1d_04	MI1d_05	MI1d_06	MI1d_07	MI7d_01	MI7d_02	MI7d_03	MI7d_04	MI7d_05	MI7d_06	MI7d_07	MI14d_01	MI14d_02	MI14d_03	MI14d_04	MI14d_05	MI14d_06	MI14d_07	MI28d_01	MI28d_02	MI28d_03	MI28d_04	MI28d_05	MI28d_06	MI28d_07	MI0d_01_arachidonic acid	MI0d_02_arachidonic acid	MI0d_03_arachidonic acid	MI0d_04_arachidonic acid	MI0d_05_arachidonic acid	MI0d_06_arachidonic acid	MI0d_07_arachidonic acid	MI1d_01_arachidonic acid	MI1d_02_arachidonic acid	MI1d_03_arachidonic acid	MI1d_04_arachidonic acid	MI1d_05_arachidonic acid	MI1d_06_arachidonic acid	MI1d_07_arachidonic acid	MI7d_01_arachidonic acid	MI7d_02_arachidonic acid	MI7d_03_arachidonic acid	MI7d_04_arachidonic acid	MI7d_05_arachidonic acid	MI7d_06_arachidonic acid	MI7d_07_arachidonic acid	MI14d_01_arachidonic acid	MI14d_02_arachidonic acid	MI14d_03_arachidonic acid	MI14d_04_arachidonic acid	MI14d_05_arachidonic acid	MI14d_06_arachidonic acid	MI14d_07_arachidonic acid	MI28d_01_arachidonic acid	MI28d_02_arachidonic acid	MI28d_03_arachidonic acid	MI28d_04_arachidonic acid	MI28d_05_arachidonic acid	MI28d_06_arachidonic acid	MI28d_07_arachidonic acid
Factors	treatment:post-MI day0 | Sample source:heart	treatment:post-MI day0 | Sample source:heart	treatment:post-MI day0 | Sample source:heart	treatment:post-MI day0 | Sample source:heart	treatment:post-MI day0 | Sample source:heart	treatment:post-MI day0 | Sample source:heart	treatment:post-MI day0 | Sample source:heart	treatment:post-MI day1 | Sample source:heart	treatment:post-MI day1 | Sample source:heart	treatment:post-MI day1 | Sample source:heart	treatment:post-MI day1 | Sample source:heart	treatment:post-MI day1 | Sample source:heart	treatment:post-MI day1 | Sample source:heart	treatment:post-MI day1 | Sample source:heart	treatment:post-MI day7 | Sample source:heart	treatment:post-MI day7 | Sample source:heart	treatment:post-MI day7 | Sample source:heart	treatment:post-MI day7 | Sample source:heart	treatment:post-MI day7 | Sample source:heart	treatment:post-MI day7 | Sample source:heart	treatment:post-MI day7 | Sample source:heart	treatment:post-MI day14 | Sample source:heart	treatment:post-MI day14 | Sample source:heart	treatment:post-MI day14 | Sample source:heart	treatment:post-MI day14 | Sample source:heart	treatment:post-MI day14 | Sample source:heart	treatment:post-MI day14 | Sample source:heart	treatment:post-MI day14 | Sample source:heart	treatment:post-MI day28 | Sample source:heart	treatment:post-MI day28 | Sample source:heart	treatment:post-MI day28 | Sample source:heart	treatment:post-MI day28 | Sample source:heart	treatment:post-MI day28 | Sample source:heart	treatment:post-MI day28 | Sample source:heart	treatment:post-MI day28 | Sample source:heart	treatment:post-MI day0 | Sample source:heart	treatment:post-MI day0 | Sample source:heart	treatment:post-MI day0 | Sample source:heart	treatment:post-MI day0 | Sample source:heart	treatment:post-MI day0 | Sample source:heart	treatment:post-MI day0 | Sample source:heart	treatment:post-MI day0 | Sample source:heart	treatment:post-MI day1 | Sample source:heart	treatment:post-MI day1 | Sample source:heart	treatment:post-MI day1 | Sample source:heart	treatment:post-MI day1 | Sample source:heart	treatment:post-MI day1 | Sample source:heart	treatment:post-MI day1 | Sample source:heart	treatment:post-MI day1 | Sample source:heart	treatment:post-MI day7 | Sample source:heart	treatment:post-MI day7 | Sample source:heart	treatment:post-MI day7 | Sample source:heart	treatment:post-MI day7 | Sample source:heart	treatment:post-MI day7 | Sample source:heart	treatment:post-MI day7 | Sample source:heart	treatment:post-MI day7 | Sample source:heart	treatment:post-MI day14 | Sample source:heart	treatment:post-MI day14 | Sample source:heart	treatment:post-MI day14 | Sample source:heart	treatment:post-MI day14 | Sample source:heart	treatment:post-MI day14 | Sample source:heart	treatment:post-MI day14 | Sample source:heart	treatment:post-MI day14 | Sample source:heart	treatment:post-MI day28 | Sample source:heart	treatment:post-MI day28 | Sample source:heart	treatment:post-MI day28 | Sample source:heart	treatment:post-MI day28 | Sample source:heart	treatment:post-MI day28 | Sample source:heart	treatment:post-MI day28 | Sample source:heart	treatment:post-MI day28 | Sample source:heart
8_9-EEQ	145.234	83.161	113.588	123.774	99.370	85.952	41.989	220.713	69.605	91.523	52.949	62.434	52.294	49.005	32.664	83.297	33.804	25.037	34.230	12.621	65.022	6.549	34.163	159.237	57.060	78.701	28.048	19.462	4.771	152.362	6.733	0.938	35.275	62.218	52.711																																			
15-deoxy-PGJ2	3.254	30.335	4.483	10.998	3.143	37.399	15.695	0.000	8.436	2.990	4.760	21.619	17.972	5.500	0.000	6.461	0.000	0.322	3.212	33.545	28.292	0.000	10.764	5.456	33.181	0.095	1.842	0.000	0.000	44.590	26.048	6.260	47.339	8.479	17.995																																			
18-HEPE	870.120	721.662	1252.477	1581.373	877.135	1536.366	459.795	1952.456	801.807	643.954	944.256	1226.566	499.911	1009.476	1046.917	758.594	65.170	432.848	906.211	1378.857	1055.031	976.436	1689.754	925.696	761.505	684.178	1076.822	204.205	927.203	2247.890	290.000	887.586	608.895	1049.883	1009.424																																			
15-HEPE	162.171	173.696	374.382	217.092	242.431	453.841	110.151	412.323	347.112	173.551	318.398	402.414	179.682	302.583	154.881	314.104	6.879	333.096	114.164	192.668	286.924	328.327	313.534	257.341	141.618	327.825	203.002	297.598	621.243	443.935	248.861	167.383	253.489	132.773	301.781																																			
11_12-EEQ	1313.840	911.850	2125.000	1210.942	2461.015	2672.443	506.023	4940.393	2436.035	4498.212	1464.030	3418.184	2609.447	3616.828	3791.005	2716.004	1521.735	3272.157	3476.315	1158.139	1375.787	2259.625	3190.400	1449.484	3455.926	2107.376	2036.647	880.688	2220.318	6537.439	3771.380	3594.647	2150.149	1481.279	2312.368																																			
9-HEPE	93.051	8.987	86.849	125.048	114.269	103.704	15.874	36.478	68.297	80.593	78.782	112.153	1.199	13.026	37.682	57.053	3.213	167.776	53.409	139.371	129.067	107.379	70.285	9.430	53.486	51.194	66.000	69.331	101.087	101.514	55.852	27.436	68.202	122.479	65.360																																			
15-oxo-ETE	4691.746	3515.858	4409.667	9723.034	4233.238	4718.953	5199.409	5248.982	4372.881	3437.193	3138.092	4867.501	6541.571	5851.772	3123.783	3080.172	387.345	4316.229	3766.541	4469.615	4728.191	4162.276	4027.411	3544.375	3876.422	5475.088	2323.243	4538.596	2576.381	6224.821	2906.903	3379.755	2277.925	4593.228	6847.647																																			
5-HEPE	24.635	24.385	92.649	48.700	96.844	139.473	27.908	133.511	5.091	24.809	13.060	0.450	46.361	35.606	48.790	80.655	72.365	95.359	25.862	44.948	85.974	31.394	20.040	131.718	149.492	120.746	29.303	47.622	155.233	73.304	79.493	21.071	16.468	87.937	95.739																																			
5-oxo-ETE	2149.816	1772.946	3207.909	5825.071	3031.749	3899.932	3013.808	3937.123	4304.253	4997.113	2268.246	4426.602	3206.956	3368.933	2899.478	2303.327	186.748	2667.754	2709.821	3715.731	2105.097	3056.951	3897.865	7179.393	3253.134	5771.941	3221.978	3291.667	2447.254	8805.430	2958.774	3364.347	2703.251	4329.993	5682.320																																			
8-HEPE	102.815	67.499	67.340	108.548	77.573	184.632	77.065	238.826	15.277	158.635	195.825	35.309	147.754	95.359	100.919	90.187	4.726	80.420	114.214	105.528	74.806	194.880	162.034	272.123	76.157	158.934	89.064	151.110	152.009	239.340	51.606	85.083	122.955	112.888	109.530																																			
11-HEPE	104.654	57.812	165.240	256.567	116.143	143.692	181.336	197.933	118.575	147.661	82.635	140.637	117.869	122.083	151.867	229.799	22.534	69.341	148.058	219.534	197.922	177.603	100.694	247.595	103.569	93.050	162.831	98.687	216.604	160.347	211.142	95.673	186.847	112.966	71.100																																			
12-HEPE	1251.421	1535.505	2431.783	1523.539	2499.563	3330.592	508.178	4245.738	2386.681	4422.849	2036.566	3600.783	2193.398	2927.091	4027.498	2316.531	2068.757	2546.160	3775.104	1570.539	1209.703	2914.187	4852.487	2189.400	3865.183	2891.906	2681.647	959.452	2341.559	8093.965	2203.465	3270.309	3032.676	1358.488	1583.736																																			
14_15-EEQ	85.366	29.085	68.767	19.734	17.131	65.871	113.748	136.500	62.257	23.552	23.300	31.019	48.403	86.865	69.681	1.006	0.000	16.342	49.696	33.718	25.504	60.746	45.284	18.975	45.769	118.487	19.045	59.010	26.755	0.000	82.590	17.446	1.081	12.345	117.946																																			
17_18-EEQ	5.874	7.114	0.618	0.000	0.000	0.000	7.882	51.818	0.819	0.000	0.000	0.000	10.623	7.003	2.537	0.000	0.000	8.757	26.817	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	17.283	0.000	0.000	1.922	0.000																																			
5_6-EET	207.603	162.542	286.351	400.454	285.061	154.037	266.035	321.317	376.660	435.241	256.183	382.980	198.874	363.853	220.979	114.484	0.000	135.915	93.962	289.613	41.154	265.978	512.846	97.769	219.809	392.590	108.001	242.762	75.366	829.552	385.015	198.644	115.956	137.793	387.515																																			
5-HETE	5297.175	2738.458	6296.160	9085.187	4768.263	9125.578	3665.369	7255.569	6424.281	6311.074	4440.861	5339.141	7364.726	5595.993	4596.611	5847.837	508.426	8386.588	4869.138	5375.465	6326.637	7173.782	6262.965	8131.649	8573.297	5273.586	4285.287	5271.724	5082.116	6466.706	6197.038	5158.273	4480.049	7785.077	8127.192																																			
19-HETE	379.664	592.792	852.399	867.447	912.164	1171.411	597.015	841.277	644.982	667.004	470.537	558.867	836.341	522.012	296.136	667.668	0.840	732.373	580.967	833.983	774.745	891.778	998.554	1383.718	908.724	989.982	473.145	828.941	801.490	237.037	704.460	483.827	827.988	764.209	1004.726																																			
18-HETE	68.490	63.619	10.343	44.927	8.067	72.864	9.349	27.458	52.171	57.592	3.131	48.345	5.664	10.000	2.334	13.471	0.000	37.222	72.944	113.770	16.860	104.160	110.893	41.356	49.537	97.924	20.435	4.935	17.212	57.217	9.641	1.746	19.141	65.535	46.619																																			
17-HETE	4451.260	4201.710	6711.877	8445.565	6684.075	11483.770	4012.893	6117.746	5512.959	6510.618	5515.374	6461.075	6430.997	5666.648	4749.180	6017.194	166.272	7017.764	5728.115	7839.886	7016.298	7198.960	11084.273	12124.587	9188.020	8460.219	5655.751	7709.362	6799.662	5911.077	6213.258	5521.948	9514.882	6829.804	9341.381																																			
16-HETE	206.346	180.281	323.323	450.488	256.197	265.269	261.911	343.441	352.678	343.242	215.236	361.599	250.974	97.113	199.798	216.244	0.000	331.169	275.087	567.276	260.367	421.984	646.822	484.135	308.311	223.256	175.782	202.096	229.565	405.542	122.183	151.788	353.241	363.514	369.496																																			
15-HETE	4005.880	4711.441	4390.870	6626.638	4624.870	7571.286	4900.844	4656.946	4913.964	3724.701	4384.823	5218.829	5482.363	4574.256	3679.760	4613.758	661.849	6040.556	4923.860	5335.035	5518.149	6540.933	7315.345	9741.545	7113.833	5536.395	4401.866	5848.883	3999.140	6173.547	4861.459	3313.291	5995.337	3754.606	6428.196																																			
11-HETE	12540.113	8635.332	13576.727	19620.401	11511.209	16161.398	8460.521	14705.734	14951.028	12464.310	12251.957	13032.161	16843.266	14952.611	12937.869	14686.035	1834.033	19979.005	12983.496	12420.981	14735.260	13717.035	13416.393	15751.548	16289.991	13647.548	9215.629	13582.063	9724.916	14690.050	18996.953	12029.458	11466.440	14404.948	16425.313																																			
8-HETE	5299.183	3489.807	6783.652	7291.716	5850.375	7462.883	2323.031	10154.899	6877.786	8126.715	4983.304	7634.305	9339.842	7891.829	7938.331	6604.278	3183.804	10201.984	8135.018	4511.348	6561.556	6814.177	10758.639	6932.520	11311.731	7384.512	5448.667	4845.752	4986.712	10892.105	9900.372	7638.672	5878.702	5362.510	6821.206																																			
9-HETE	1272.589	322.302	1265.931	1860.833	1177.595	1623.088	855.084	1619.596	1291.497	1339.815	758.072	1304.202	1747.594	1152.641	1027.624	980.369	9.632	1399.593	1367.799	1217.726	1355.554	1497.700	1462.620	1640.640	1776.115	1421.370	1172.203	960.852	1213.100	1155.396	1479.563	1158.839	1063.688	1525.757	1375.673																																			
8_9-EET	732.423	1038.249	681.181	717.997	714.545	1000.590	896.201	660.130	608.571	445.963	754.051	667.321	895.375	761.529	691.783	829.839	979.445	858.457	776.135	763.435	913.098	892.379	787.591	693.000	848.526	612.689	593.583	832.118	867.486	428.251	505.710	589.772	1247.076	609.760	718.125																																			
14_15-EET	621.409	638.168	541.676	853.233	463.111	698.071	722.550	118.913	170.198	140.337	93.289	151.235	124.641	45.116	206.601	139.710	178.098	213.017	324.783	344.728	364.705	276.898	232.493	249.620	143.561	310.229	102.460	267.259	38.941	81.777	76.755	56.302	33.749	36.895	74.921																																			
11_12-EET	459.527	480.319	446.280	497.616	384.284	479.337	368.414	156.513	176.673	228.010	27.845	81.326	57.465	90.557	125.831	146.235	214.668	148.863	249.500	263.973	208.185	247.655	253.676	58.981	111.634	324.694	99.467	144.654	98.447	150.764	39.466	35.154	51.305	61.184	397.107																																			
12-HETE	19688.173	25547.963	43297.431	26784.430	43320.686	59333.130	13202.723	52331.313	40470.840	59117.928	36754.195	62611.769	51011.401	52388.748	59708.696	43010.891	47160.461	59965.106	65393.749	33417.648	30101.487	54685.349	117396.267	72451.518	81348.907	61376.095	48899.495	24731.142	30141.892	100102.423	54143.122	47040.207	59110.033	25224.339	41740.237																																			
PGB3	41.097	25.233	43.610	75.526	51.841	50.047	164.920	46.659	5.009	29.646	93.310	39.925	51.559	43.590	10.572	13.921	1.096	39.563	43.827	94.042	82.157	88.218	105.578	73.989	77.466	37.818	161.912	85.655	44.711	79.974	93.030	25.380	38.863	169.977	108.192																																			
PGJ2	0.013	0.009	0.009	0.036	0.015	0.026		0.021	0.021		0.005		0.026	0.012	0.033	0.011	0.013	0.010	0.038	0.032	0.004	0.014	0.025	0.002	0.021	0.041	0.013		0.010	0.016	0.025	0.018	0.003	0.006	0.023																																			
PGB2	0.004	0.001	0.002	0.002	0.003	0.009	0.003	0.002	0.001		0.002	0.000	0.003	0.003	0.001	0.001	0.000	0.001	0.000	0.002	0.003	0.009		0.006		0.005	0.002	0.004	0.003	0.001	0.004	0.001	0.000	0.002	0.009																																			
5_6-diHETE	0.282	0.040	0.014	0.672	0.373	0.263	0.248	0.018	0.332	0.404	0.071	0.000	0.197	0.159	0.031	0.189	0.106	0.190	0.248	0.372	0.078	0.459	0.254	0.101	0.164	0.133	0.242	0.120	0.371	0.000	0.202	0.515	0.017	0.272	0.046																																			
17_18-DiHETE	0.007	0.009	0.005	0.008	0.013	0.001	0.006	0.011	0.007	0.005	0.012	0.031	0.018	0.014	0.003	0.008	0.013	0.006	0.011	0.006	0.017	0.010	0.008	0.007	0.008	0.001	0.011	0.016	0.006	0.008	0.001	0.013	0.004	0.004	0.006																																			
14_15-DiHETE	0.005	0.001	0.007	0.038	0.005	0.016	0.006	0.028	0.030	0.033	0.002	0.031	0.005	0.057	0.002	0.024	0.007	0.029	0.035	0.008	0.019	0.034	0.003	0.007	0.011	0.010	0.008	0.008	0.025	0.033	0.007	0.033	0.028	0.037	0.026																																			
LTB4	2.034	2.016	2.089	2.046	1.865	2.134	1.953	11.986	14.328	11.191	11.877	11.364	11.025	10.896	4.697	5.069	5.155	5.496	7.318	6.275	6.217	5.504	4.471	5.090	5.327	4.502	4.567	4.942	1.841	2.386	2.188	1.493	1.787	1.548	1.521																																			
5_6-DHET	360.356	211.651	431.174	358.122	310.190	624.892	547.840	349.050	281.201	260.937	271.905	429.831	229.702	152.212	150.971	304.661	175.167	542.464	292.620	234.332	293.181	1106.958	1145.889	1570.643	795.171	571.698	456.264	449.378	611.456	580.418	479.301	449.653	803.807	692.988	843.458																																			
8_9-DHET	1051.937	553.058	1049.021	771.858	1106.403	1944.180	1512.074	606.097	900.432	775.812	890.753	1028.778	1051.280	953.923	706.612	1063.255	34.514	1294.834	904.609	1251.985	1098.977	2244.910	2103.247	2523.860	1870.804	1165.383	1255.920	1532.404	1388.684	2085.220	1139.444	1295.839	2054.237	1905.582	2207.391																																			
14_15-DHET	3761.991	2676.643	6965.074	4563.240	4428.492	5659.750	4456.284	5139.056	3722.575	4664.212	4131.687	7398.035	4943.597	4059.160	2440.388	5030.741	68.965	2778.410	6812.396	8635.370	9749.204	7486.749	7591.773	5888.574	8107.463	5378.802	4736.699	3973.460	5757.164	14346.418	5286.623	5389.525	2162.411	5572.378	6729.607																																			
11_12-DHET	15596.606	12204.236	26339.835	16758.952	16813.141	25346.002	19595.501	21804.252	17682.152	23814.741	16539.958	31121.587	21381.295	15166.003	11611.996	19651.460	1122.848	11467.257	28144.742	33093.314	35327.124	31138.379	37102.709	26772.811	33739.283	23368.440	29537.677	17682.750	22116.912	49104.336	23405.441	19618.535	13009.211	24642.451	27337.054																																			
LTB4_D4	22788.111	22606.441	21597.027	19457.111	21794.449	18207.166	22015.932	19860.848	17253.717	22103.725	18645.807	18582.594	19840.736	19023.098	22207.002	20989.998	22337.492	18517.748	15256.333	16198.901	17732.631	13444.258	15941.419	13745.511	15552.785	16477.891	16597.398	15655.517	18864.832	11185.082	14406.593	19337.582	17707.924	19424.832	18331.719																																			
4-HDoHE	6.196	2.404	6.418	9.273	6.880	9.368	3.501	7.572	7.190	7.054	6.156	6.352	7.698	7.694	6.181	5.038	0.182	6.414	6.087	6.506	5.898	4.384	4.170	4.071	4.019	4.979	3.970	5.756	5.107	9.392	5.210	5.300	4.893	7.360	6.796																																			
8-HDoHE	2.323	0.810	2.372	3.054	2.541	2.949	0.952	2.385	1.903	2.579	1.690	2.218	2.649	2.992	1.991	1.900	0.059	2.341	2.356	1.879	2.089	1.457	1.261	1.606	1.268	2.078	1.451	2.316	1.734	2.896	2.679	2.027	1.902	2.576	2.332																																			
20-HDoHE	267.777	96.642	210.324	154.633	291.371	216.843	190.931	89.280	171.328	296.018	143.794	291.434	152.729	242.221	173.321	144.026	9.200	153.142	173.418	182.016	197.446	184.352	156.058	378.486	213.648	172.473	42.031	192.054	208.699	260.356	182.426	124.572	262.003	91.294	287.911																																			
16-HDoHE	2.979	3.147	3.903	4.824	3.496	6.500	2.541	3.432	3.734	2.785	3.896	3.841	4.192	4.099	3.390	2.584	0.063	3.339	3.725	4.843	4.021	3.222	3.258	4.934	2.964	3.397	3.121	3.210	3.332	3.912	3.016	2.402	3.741	2.985	4.145																																			
17-HDoHE	3.397	2.903	3.549	4.900	5.339	6.467	2.826	2.939	3.646	2.895	3.952	4.152	4.071	3.504	3.610	2.393	0.082	3.645	4.099	4.558	3.843	2.698	3.370	4.590	2.806	4.410	2.748	3.433	3.738	4.496	3.508	2.109	4.775	2.932	4.553																																			
13-HDoHE	1.866	1.533	2.006	2.435	2.427	3.454	1.502	1.749	2.051	1.790	2.410	2.551	2.780	2.009	2.105	1.799	0.121	1.827	2.213	2.514	1.784	1.328	1.955	2.555	1.524	2.345	1.383	2.483	1.692	3.212	0.993	1.253	2.818	1.220	1.672																																			
14-HDoHE	1.755	0.982	2.138	2.340	2.622	2.524	0.665	3.691	2.834	2.979	1.979	3.643	2.881	3.943	3.555	1.802	1.178	2.821	3.568	1.644	1.838	1.682	3.028	1.808	2.490	2.046	1.604	2.207	1.696	5.766	3.444	1.936	1.956	1.556	2.414																																			
10-HDoHE	0.122	0.033	0.150	0.221	0.144	0.285	0.013	0.154	0.103	0.146	0.158	0.051	0.042	0.165	0.089	0.057	0.007	0.197	0.114	0.049	0.150	0.065	0.043	0.151	0.121	0.126	0.108	0.197	0.148	0.342	0.152	0.064	0.209	0.142	0.164																																			
11-HDoHE	2.684	1.065	2.666	3.308	2.417	3.351	1.080	2.304	2.578	2.302	1.860	2.185	2.983	2.588	2.060	1.725	0.073	2.865	1.887	2.702	3.167	1.980	1.673	2.231	1.585	1.999	1.553	2.181	1.937	2.729	2.062	2.255	1.743	3.003	2.506																																			
7-HDoHE	0.969	0.521	1.029	1.554	1.243	1.551	0.611	1.130	1.232	1.239	0.814	0.976	1.207	1.397	1.093	0.916	0.050	1.085	0.937	1.024	1.163	0.551	0.650	0.903	0.517	0.778	0.814	0.976	1.069	1.326	1.328	0.825	0.828	1.305	1.206																																			
19_20-EDP	0.130	0.144	0.180	0.320	0.197	0.224	0.210	0.215	0.300	0.211	0.320	0.245	0.206	0.251	0.182	0.170	0.032	0.192	0.084	0.153	0.168	0.218	0.085	0.243	0.086	0.177	0.099	0.182	0.096	0.366	0.249	0.102	0.074	0.135	0.144																																			
16_17-EDP	14.479	4.598	18.963	118.827	3.866	30.031	1.352	42.679	2.654	12.446	24.993	55.671	12.317	6.107	4.919	5.527	0.000	11.190	95.791	63.221	1.323	3.038	0.000	18.181	0.000	31.511	18.760	11.958	19.573	21.143	40.626	11.118	50.119	28.913	20.245																																			
11_12-DHET_D11	1.764	0.241	2.736	2.169	1.511	0.000	2.431	1.028	2.238	0.931	0.225	1.812	3.367	9.429	1.002	2.474	3.468	0.000	0.056	0.116	1.061	0.771	4.998	0.000	0.499	2.741	1.068	0.000	5.749	11.290	15.511	11.998	6.034	1.130	3.480																																			
PGD3	2.821	1.748	2.081	3.396	4.430	0.000	20.738	3.277	2.491	2.408	10.261	8.399	2.962	1.557	0.000	0.000	0.000	1.172	6.182	0.000	2.595	2.240	11.615	0.000	0.000	0.000	11.811	1.886	0.000	0.000	0.000	0.000	0.000	0.000	0.841																																			
PGE3	13.117	15.588	65.566	47.294	49.727	83.847	30.594	50.900	33.458	35.469	26.499	53.187	34.848	85.094	8.090	30.953	5.564	25.514	51.566	80.392	43.215	7.441	66.721	51.477	49.037	78.550	24.903	102.988	23.431	110.104	26.597	23.565	34.675	37.895	56.803																																			
PGD2	9.479	41.741	29.347	12.434	34.521	11.493	6.507	9.488	7.282	24.038	7.059	9.136	1.471	52.159	12.622	21.886	2.281	2.884	16.366	11.089	14.695	1.564	59.170	10.510	2.359	57.771	0.000	5.649	19.300	2.372	0.976	22.084	10.936	12.153	16.743																																			
LXA4	1.921	1.972	9.125	5.299	3.223	0.611	0.132	2.730	5.185	7.815	1.827	0.937	3.361	1.001	1.863	3.135	0.932	2.064	2.395	9.570	1.907	1.519	2.354	1.791	4.426	0.828	7.230	10.020	6.483	0.192	4.930	0.921	5.317	7.849	2.288																																			
PGF3a	32.284	27.280	81.734	56.408	19.483	41.602	206.842	6.039	40.793	57.573	59.833	36.928	38.465	41.063	56.981	77.110	28.431	42.120	68.235	73.310	17.202	43.224	18.470	143.805	71.377	131.875	6.508	0.000	24.923	52.042	80.441	48.086	22.648	51.452	19.872																																			
PGE2	194.839	217.341	384.622	212.824	179.738	188.694	164.625	266.525	313.471	249.233	362.530	271.946	247.387	514.084	63.012	146.227	53.975	340.855	211.403	147.299	99.656	58.840	288.174	176.955	234.349	565.297	241.443	265.567	157.169	1008.601	440.417	345.269	249.505	624.251	446.256																																			
PGF2a	11628.638	12543.006	12895.119	11509.522	12840.085	10663.593	10942.376	11794.960	11486.471	13505.273	9010.866	12362.218	12305.888	9415.176	12354.611	13052.010	12313.214	12650.575	9454.892	10282.303	8314.381	7726.943	9731.007	7679.880	10140.486	9155.827	10532.944	9372.955	9989.011	2457.402	7274.753	10231.209	9843.948	8518.536	10854.904																																			
maresin-1	0.008	0.004	0.012	0.006	0.025	0.025	0.011	0.020	0.025	0.002	0.016	0.026	0.006	0.020	0.002	0.029		0.018	0.025	0.055	0.029	0.005	0.023	0.027	0.005	0.062	0.017	0.025	0.009	0.031	0.012	0.024	0.029	0.009	0.003																																			
TXB3	0.185	0.954	0.000	11.935	5.619	3.926	4.481	2.590	0.797	0.223	0.265	3.667	0.432	1.758	2.189	2.835	0.000	0.000	13.238	0.583	1.348	1.411	5.735	6.524	1.196	1.447	0.579	5.241	1.242	20.473	0.000	0.000	0.000	6.107	1.767																																			
TXB2	0.198	0.061	0.396	0.084	0.202	0.146	0.111	0.244	0.142	0.313	0.373	0.351	0.131	0.596	1.000	0.163	0.133	0.258	0.259	0.374	0.173	0.417	0.361	0.199	0.745	1.069	0.129	0.035	0.032	0.982	1.182	0.534	0.149	0.251	0.244																																			
6-keto-PGF1a	27.075	29.037	85.804	34.492	45.726	59.833	21.325	48.292	47.414	54.662	80.261	35.389	43.529	65.842	71.435	82.011	37.019	61.542	60.667	95.508	53.167	55.546	68.693	86.287	90.629	66.289	41.027	72.133	12.237	103.758	183.426	71.424	69.104	57.501	70.200																																			
resolvin-D2	0.003	0.011	0.009	0.004	0.011	0.012	0.007	0.002	0.008	0.012	0.004	0.005	0.002	0.006	0.006	0.011	0.002	0.031	0.009	0.008	0.003	0.000	0.004	0.002	0.004	0.002	0.003	0.002	0.013	0.029	0.019	0.001	0.020	0.009	0.002																																			
resolvin-D1	4.721	17.851	0.000	41.234	1.828	3.000	19.609	7.411	0.000	41.240	9.473	5.826	7.887	0.000	11.716	7.399	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	1.855	0.000	0.000	0.000	1.686	30.415	0.000	0.000	0.319	0.000	14.467																																			
9,10-DiHOME	228143.359	251238.516	238090.781	287547.906	294068.844	241662.234	270759.344	199572.172	180594.609	278332.438	188472.875	253214.000	180318.078	231065.953	128793.984	233430.719	12788.190	205967.234	212123.875	259261.609	187000.422	191219.953	204895.984	181083.141	203644.359	213378.828	196437.188	246095.563	341506.969	125626.477	198098.719	226512.109	247403.828	278836.188	256429.375																																			
9,10-EpOME	6536.833	6798.370	9442.864	15566.561	16330.345	11456.669	3021.110	15824.825	6986.457	16252.532	7265.095	8065.303	7295.003	12986.736	6765.080	5107.007	1486.310	4614.579	6496.776	10277.922	5227.574	5910.321	5225.042	4040.724	6732.797	17591.258	5200.639	5230.169	11148.266	13709.180	5327.918	11052.243	13467.660	9404.865	12677.221																																			
12,13-DiHOME	40231.977	52270.488	42037.344	58137.980	61847.922	49766.844	49867.145	35669.965	32523.285	59094.449	35487.539	46811.289	31699.725	45446.668	26075.713	44739.895	4884.681	38642.445	38922.574	50820.266	32811.898	35398.660	38058.457	32509.795	39804.902	43475.281	37389.539	45330.844	67173.609	23394.713	40825.695	45777.652	45063.176	56083.898	47975.371																																			
12,13-EpOME	3709.741	6543.717	4949.856	16418.934	7786.644	3927.740	1069.502	8748.056	6713.585	12859.715	4970.212	4277.222	2381.556	6633.581	3053.802	2127.796	331.418	2390.689	3780.287	8496.235	3276.266	2575.883	5037.434	1771.399	2961.417	6905.572	1964.290	4924.234	7376.139	4307.253	1841.169	3535.008	3711.006	3548.366	5090.918																																			
10(s),17(s)-DiHOME	771.105	325.060	1056.370	1060.927	1593.368	1263.408	421.826	712.568	894.362	844.492	520.151	436.524	410.576	565.542	492.740	888.404		853.871	440.052	928.470	802.345	240.612	355.695	172.801	347.897	653.837	335.484	541.069	273.957	252.542	791.142	398.644	367.142	683.153	673.935																																			
ARA																																				60.154	84.054	42.152	83.450	57.156	68.310	65.156	135.591	157.678	132.236	151.215	138.202	142.426	172.970	100.361	102.435	78.354	119.486	71.416	106.742	96.660	85.772	93.550	82.547	72.063	55.879	79.770	64.420	60.454	43.726	56.753	68.972	47.967	104.317	81.580
MS_METABOLITE_DATA_END
#METABOLITES
METABOLITES_START
metabolite_name	Pubchem ID	Kegg ID
8_9-EEQ
15-deoxy-PGJ2
18-HEPE	16061132
15-HEPE	16061131
11_12-EEQ
9-HEPE	16061129
15-oxo-ETE	5280701
5-HEPE	6439678
5-oxo-ETE	5283159
8-HEPE	16061128
11-HEPE	16061130
12-HEPE	10041593
14_15-EEQ	472955894
17_18-EEQ	16061089
5_6-EET	5283202	C14768
5-HETE	5280733	C04805
19-HETE	20839325
18-HETE	6442778
17-HETE	6442740
16-HETE	6442739
15-HETE	9966861	C21482
11-HETE	14123410
8-HETE	11976122
9-HETE	14123398
8_9-EET	5283203	C14769
14_15-EET	5283205	C14771
11_12-EET	5283204	C14770
12-HETE	13786989
PGB3	5283113
PGJ2	5280884	C05957
PGB2	5280881	C05954
5_6-diHETE	14429105
17_18-DiHETE	16061120
14_15-DiHETE	16061119
LTB4	5280492	C02165
5_6-DHET	5283142	C14772
8_9-DHET	5283144	C14773
14_15-DHET	5283147	C14775
11_12-DHET	5283146	C14774
LTB4_D4
4-HDoHE	14429109
8-HDoHE	11976798
20-HDoHE	16061143
16-HDoHE	11595378
17-HDoHE	6439179
13-HDoHE	11559259
14-HDoHE	11566378
10-HDoHE	11537494
11-HDoHE	11631564
7-HDoHE	16061142
19_20-EDP	11631565
16_17-EDP	14392758
11_12-DHET_D11
PGD3	5282260	C13802
PGE3	5280937	C06439
PGD2	448457	C00696
LXA4	5280914	C06314
PGF3a	5283217
PGE2	5280360	C00584
PGF2a	5280363	C00639
maresin-1	102518309
TXB3	5283140
TXB2	5283137	C05963
6-keto-PGF1a	5280888	C05961
resolvin-D2	11383310
resolvin-D1	44251266
9,10-DiHOME	9966640	C14828
9,10-EpOME	6246154
12,13-DiHOME	10236635
12,13-EpOME	5356421
10(s),17(s)-DiHOME
ARA	444899	C00219
METABOLITES_END
#END