#METABOLOMICS WORKBENCH gunjan27_20241126_112739 DATATRACK_ID:5408 STUDY_ID:ST003597 ANALYSIS_ID:AN005909 PROJECT_ID:PR002225
VERSION             	1
CREATED_ON             	November 26, 2024, 4:48 pm
#PROJECT
PR:PROJECT_TITLE                 	Metabolic regulation of RNA methylation by the m6A-reader IGF2BP3
PR:PROJECT_SUMMARY               	The interplay of RNA modifications – deposited by “writers”, removed by
PR:PROJECT_SUMMARY               	“erasers” and identified by RNA binding proteins known as “readers” –
PR:PROJECT_SUMMARY               	forms the basis of the epitranscriptomic gene regulation hypothesis. Recent
PR:PROJECT_SUMMARY               	studies have identified the oncofetal RNA-binding protein IGF2BP3 as a
PR:PROJECT_SUMMARY               	“reader” of the N6-methyladenosine (m6A) modification and crucial for
PR:PROJECT_SUMMARY               	regulating gene expression. Here, we report the novel finding that the
PR:PROJECT_SUMMARY               	“reader” IGF2BP3 changes cellular metabolism, resulting in an altered
PR:PROJECT_SUMMARY               	ability of the “writers” to modify the epitranscriptome. Specifically, loss
PR:PROJECT_SUMMARY               	of IGF2BP3 caused a reduction in glycolytic flux and one-carbon metabolism,
PR:PROJECT_SUMMARY               	leading to decreased production of S-adenosyl methionine, a key substrate for
PR:PROJECT_SUMMARY               	methylation reactions within the cell.
PR:INSTITUTE                     	University of California, Los Angeles
PR:LAST_NAME                     	Rao
PR:FIRST_NAME                    	Dinesh S
PR:ADDRESS                       	12-272, Factor Building, 650 Charles E Young Drive, UCLA, USA 90095
PR:EMAIL                         	gunjansharma@mednet.ucla.edu
PR:PHONE                         	3108252548
#STUDY
ST:STUDY_TITLE                   	Metabolic regulation of RNA methylation by the m6A-reader IGF2BP3
ST:STUDY_SUMMARY                 	The interplay of RNA modifications – deposited by “writers”, removed by
ST:STUDY_SUMMARY                 	“erasers” and identified by RNA binding proteins known as “readers” –
ST:STUDY_SUMMARY                 	forms the basis of the epitranscriptomic gene regulation hypothesis. Recent
ST:STUDY_SUMMARY                 	studies have identified the oncofetal RNA-binding protein IGF2BP3 as a
ST:STUDY_SUMMARY                 	“reader” of the N6-methyladenosine (m6A) modification and crucial for
ST:STUDY_SUMMARY                 	regulating gene expression. Here, we report the novel finding that the
ST:STUDY_SUMMARY                 	“reader” IGF2BP3 changes cellular metabolism, resulting in an altered
ST:STUDY_SUMMARY                 	ability of the “writers” to modify the epitranscriptome. Specifically, loss
ST:STUDY_SUMMARY                 	of IGF2BP3 caused a reduction in glycolytic flux and one-carbon metabolism,
ST:STUDY_SUMMARY                 	leading to decreased production of S-adenosyl methionine, a key substrate for
ST:STUDY_SUMMARY                 	methylation reactions within the cell.
ST:INSTITUTE                     	University of California, Los Angeles
ST:LAST_NAME                     	Rao
ST:FIRST_NAME                    	Dinesh S
ST:ADDRESS                       	12-272, Factor Building, 650 Charles E Young Drive, UCLA, USA 90095
ST:EMAIL                         	gunjansharma@mednet.ucla.edu
ST:PHONE                         	3108252548
#SUBJECT
SU:SUBJECT_TYPE                  	Cultured cells
SU:SUBJECT_SPECIES               	Homo sapiens
SU:TAXONOMY_ID                   	9606
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data
SUBJECT_SAMPLE_FACTORS           	IMP3NT-Ctrl1_SEM	GS-001	Sample source:Acute Lymphoblastic Leukemia | Genotype:WT | Treatment:Control	RAW_FILE_NAME=GS-001
SUBJECT_SAMPLE_FACTORS           	IMP3NT-Ctrl1_SEM	GS-002	Sample source:Acute Lymphoblastic Leukemia | Genotype:WT | Treatment:Control	RAW_FILE_NAME=GS-002
SUBJECT_SAMPLE_FACTORS           	IMP3NT-Ctrl1_SEM	GS-003	Sample source:Acute Lymphoblastic Leukemia | Genotype:WT | Treatment:Control	RAW_FILE_NAME=GS-003
SUBJECT_SAMPLE_FACTORS           	IMP3-KO1_SEM	GS-004	Sample source:Acute Lymphoblastic Leukemia | Genotype:KO | Treatment:Knockout	RAW_FILE_NAME=GS-004
SUBJECT_SAMPLE_FACTORS           	IMP3-KO1_SEM	GS-005	Sample source:Acute Lymphoblastic Leukemia | Genotype:KO | Treatment:Knockout	RAW_FILE_NAME=GS-005
SUBJECT_SAMPLE_FACTORS           	IMP3-KO1_SEM	GS-006	Sample source:Acute Lymphoblastic Leukemia | Genotype:KO | Treatment:Knockout	RAW_FILE_NAME=GS-006
SUBJECT_SAMPLE_FACTORS           	IMP3NT2-Ctrl2_SEM	GS-007	Sample source:Acute Lymphoblastic Leukemia | Genotype:WT | Treatment:Control	RAW_FILE_NAME=GS-007
SUBJECT_SAMPLE_FACTORS           	IMP3NT2-Ctrl2_SEM	GS-008	Sample source:Acute Lymphoblastic Leukemia | Genotype:WT | Treatment:Control	RAW_FILE_NAME=GS-008
SUBJECT_SAMPLE_FACTORS           	IMP3NT2-Ctrl2_SEM	GS-009	Sample source:Acute Lymphoblastic Leukemia | Genotype:WT | Treatment:Control	RAW_FILE_NAME=GS-009
SUBJECT_SAMPLE_FACTORS           	IMP3-KO2_SEM	GS-010	Sample source:Acute Lymphoblastic Leukemia | Genotype:KO | Treatment:Knockout	RAW_FILE_NAME=GS-010
SUBJECT_SAMPLE_FACTORS           	IMP3-KO2_SEM	GS-011	Sample source:Acute Lymphoblastic Leukemia | Genotype:KO | Treatment:Knockout	RAW_FILE_NAME=GS-011
SUBJECT_SAMPLE_FACTORS           	IMP3-KO2_SEM	GS-012	Sample source:Acute Lymphoblastic Leukemia | Genotype:KO | Treatment:Knockout	RAW_FILE_NAME=GS-012
#COLLECTION
CO:COLLECTION_SUMMARY            	5e6 cells were collected, rinsed with PBS, and immediately extracted in 1 ml
CO:COLLECTION_SUMMARY            	ice-cold 80% methanol (Optima* LC/MS, Fisher Scientific). As an internal
CO:COLLECTION_SUMMARY            	standard, 1 µM norvaline (Sigma-Aldrich) was added to each sample. Samples were
CO:COLLECTION_SUMMARY            	then vortexed every 5 min, three times and clarified at top speed for 5 min at 4
CO:COLLECTION_SUMMARY            	°C. The supernatants was transferred to a new microcentrifuge tube and dried
CO:COLLECTION_SUMMARY            	overnight using a refrigerated CentriVap vacuum concentrator (LabConco).
CO:COLLECTION_SUMMARY            	Extracted metabolite samples were stored at -80°C. The insoluble pellets were
CO:COLLECTION_SUMMARY            	resuspended in 0.5 M NaOH for protein estimation.
CO:SAMPLE_TYPE                   	Cultured cells
#TREATMENT
TR:TREATMENT_SUMMARY             	Cells of the control and IGF2BP3 knocked out cell lines were plated in
TR:TREATMENT_SUMMARY             	triplicates and cultured in their regular culture medium without glucose but
TR:TREATMENT_SUMMARY             	supplemented with 13C6 Glucose (10 mM, Cambridge Isotope Laboratories, Inc.) for
TR:TREATMENT_SUMMARY             	24 hours prior to metabolite extraction.
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	Dried metabolites were resuspended in 100 µl 50% ACN:water and 5 µl was loaded
SP:SAMPLEPREP_SUMMARY            	onto a Luna 3µm NH2 100A (150 × 2.0 mm) column (Phenomenex). The
SP:SAMPLEPREP_SUMMARY            	chromatographic separation was performed on a Vanquish Flex (Thermo Scientific)
SP:SAMPLEPREP_SUMMARY            	with mobile phases A (5 mM NH4AcO pH 9.9) and B (ACN) and a flow rate of 200
SP:SAMPLEPREP_SUMMARY            	μl/min. A linear gradient from 15% A to 95% A over 18 min was followed by 7 min
SP:SAMPLEPREP_SUMMARY            	isocratic flow at 95% A and re-equilibration to 15% A. Metabolites were detected
SP:SAMPLEPREP_SUMMARY            	with a Thermo Scientific Q Exactive mass spectrometer run with polarity
SP:SAMPLEPREP_SUMMARY            	switching in full scan mode with an m/z range of 70-975 and 70.000 resolution.
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_TYPE           	HILIC
CH:INSTRUMENT_NAME               	Thermo Vanquish
CH:COLUMN_NAME                   	Phenomenex Synergi Hydro RP 100 A (100 x 2mm,2.5um)
CH:SOLVENT_A                     	5 mM NH4AcO pH 9.9
CH:SOLVENT_B                     	100% Acetonitrile
CH:FLOW_GRADIENT                 	The chromatographic separation was performed on a Vanquish Flex (Thermo
CH:FLOW_GRADIENT                 	Scientific) with mobile phases A (5 mM NH4AcO pH 9.9) and B (ACN) and a flow
CH:FLOW_GRADIENT                 	rate of 200 μl/min. A linear gradient from 15% A to 95% A over 18 min was
CH:FLOW_GRADIENT                 	followed by 7 min isocratic flow at 95% A and re-equilibration to 15% A
CH:FLOW_RATE                     	0.2 mL/min
CH:COLUMN_TEMPERATURE            	27 ºC
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
#MS
MS:INSTRUMENT_NAME               	Thermo Q Exactive Orbitrap
MS:INSTRUMENT_TYPE               	Orbitrap
MS:MS_TYPE                       	ESI
MS:ION_MODE                      	NEGATIVE
MS:MS_COMMENTS                   	Metabolites were detected with a Thermo Scientific Q Exactive mass spectrometer
MS:MS_COMMENTS                   	run with polarity switching in full scan mode with an m/z range of 70-975 and
MS:MS_COMMENTS                   	70.000 resolution.
#MS_METABOLITE_DATA
MS_METABOLITE_DATA:UNITS	peak area
MS_METABOLITE_DATA_START
Samples	GS-001	GS-002	GS-003	GS-004	GS-005	GS-006	GS-007	GS-008	GS-009	GS-010	GS-011	GS-012
Factors	Sample source:Acute Lymphoblastic Leukemia | Genotype:WT | Treatment:Control	Sample source:Acute Lymphoblastic Leukemia | Genotype:WT | Treatment:Control	Sample source:Acute Lymphoblastic Leukemia | Genotype:WT | Treatment:Control	Sample source:Acute Lymphoblastic Leukemia | Genotype:KO | Treatment:Knockout	Sample source:Acute Lymphoblastic Leukemia | Genotype:KO | Treatment:Knockout	Sample source:Acute Lymphoblastic Leukemia | Genotype:KO | Treatment:Knockout	Sample source:Acute Lymphoblastic Leukemia | Genotype:WT | Treatment:Control	Sample source:Acute Lymphoblastic Leukemia | Genotype:WT | Treatment:Control	Sample source:Acute Lymphoblastic Leukemia | Genotype:WT | Treatment:Control	Sample source:Acute Lymphoblastic Leukemia | Genotype:KO | Treatment:Knockout	Sample source:Acute Lymphoblastic Leukemia | Genotype:KO | Treatment:Knockout	Sample source:Acute Lymphoblastic Leukemia | Genotype:KO | Treatment:Knockout
2-HG C12 PARENT	3125920.75	2472372.5	2980940	1368791.88	1538700	1360134.13	2199575.5	2021895	2203843.75	2000409.38	2248511	1830888.38
2-HG C13-label-2	910722.31	611311.56	740301.25	147229.64	120257.79	88592.73	260066.55	224528.5	244225.77	162559.3	202979.55	135287.11
2-HG C13-label-3	318488.41	268484.47	249794.89	10723.09	0	12014.46	42003.48	67556.66	66666.95	0	14858.26	23461.39
2-HG C13-label-4	401132.28	309361.47	357276.72	0	13498.17	0	99908.1	66323.71	18573.36	56931.32	53331.44	38192.15
2-HG C13-label-5	224433.39	223182.8	201015.14	0	0	0	80130.21	0	49210.03	0	0	0
3-Phosphoglycerate C12 PARENT	168885.55	89666.59	77212.98	68836.88	72089.19	114928.1	50355.54	110508.04	154112.05	135659.11	86060.82	89347.26
3-Phosphoglycerate C13-label-3	1690451.13	920349.19	785080.69	487089.5	1197324.5	1110236.13	1395686.63	1455024.13	1533577.5	1065940.25	1836923.38	1084089.13
4-Hydroxyphenyllactate C12 PARENT	516434.34	163551.66	289172.41	28911.22	65607.94	33629.84	120587.47	94909.02	22517.41	70637.55	247449.2	82357.32
5-Oxoproline C12 PARENT	23011674	28601770	25576896	13397623	11637139	10913659	14905417	13814421	11892175	10238161	13464855	10152931
5-Oxoproline C13-label-1	1164619.13	1627037.13	1699304.38	731356.06	825569.19	488946.59	839761.69	965906.5	738895	500789	875255.19	476317.16
5-Oxoproline C13-label-2	1626777.13	1111990.63	2132189.25	503369.41	451004.84	496622.22	842478.44	901890.94	644905.06	458992.78	655097.94	530161.06
5-Oxoproline C13-label-4	550985.31	436974.41	625391.56	115151.43	88293.52	48410.57	344578.25	338318.41	112287.88	134115.08	250302.13	148137.89
5-Oxoproline C13-label-5	279049.22	146828.75	437808.41	31216.7	24914.13	42792.17	91750.2	142157.98	40028.16	89062.93	85784.01	22359.81
6-Phosphogluconate C12 PARENT	0	0	0	0	0	0	0	0	0	0	0	0
6-Phosphogluconate C13-label-6	9785.94	169760.52	85416.59	12637.09	0	0	9942.22	0	0	0	0	0
ADP C12 PARENT	3449126	2145481	4289528.5	4335890	6463065.5	5751213.5	5135797.5	5236738.5	4669520	3311182.75	3776982	2674294
ADP C13-label-1	376263.38	187380.08	540048.69	519943.53	734539.19	607332.06	569452.56	517792.5	597740.88	369992.09	405495.53	269359.72
ADP C13-label-2	279674.59	140629.69	371857.22	291828.59	370156.22	334712.72	366301.5	354722.78	334111.13	181918.55	266977.38	178556.86
ADP C13-label-3	343117.44	174489.36	414710.41	243525.94	417873.47	349429.84	296879.09	391776.91	299728.16	231584.88	284058.69	171083.17
ADP C13-label-4	1462977	830063.94	1908585.63	875418.31	933645.25	977782.5	1403906.88	1384673.63	1181821	663275.75	823165.56	705428.19
ADP C13-label-5	19058096	10555628	23640802	10009481	13661411	12405240	19306388	18870934	16150325	11131839	12315913	8848487
ADP C13-label-6	5638342	3327604.25	6708906.5	1013560.5	1263563.88	1248297.38	3187747.75	3066142	2469514.25	1408945	1617884.38	1305606.5
ADP C13-label-7	2141881.5	1188179.63	2650000.75	334574.75	457093.5	406069.5	1132116.13	1044326.94	757987.69	418232.84	614611.19	383251.59
ADP C13-label-8	490193.59	243552.86	518353.28	52681.13	0	21206.61	172776.88	89653.91	74566.57	24318.02	13224.19	26660.51
ADP C13-label-9	15664.67	14377.95	48390.53	0	0	0	0	0	0	0	0	0
ATP C12 PARENT	13978060	5356485.5	10436406	6155525.5	12639752	10506608	10733081	9203677	10207828	6242912.5	6805700.5	4429178.5
ATP C13-label-1	1628878.63	695093.44	1156690.38	645172.38	1573029.13	1350593.25	1271789.38	1072479.63	1218052	804361.75	821459.19	480494.03
ATP C13-label-2	1403738.13	556932.88	957442.81	472310.75	822282.69	666741.69	901743.56	661743.25	928686.69	463200.03	472064.25	302343.53
ATP C13-label-3	1334462.13	559876.81	1086735.88	370596.78	786993.75	615634.69	708896.81	574640.88	690656.56	507700.13	414754.09	300997.72
ATP C13-label-4	5427014	2221660	3897015.75	1080404.13	1990961.63	1568007.5	3090946.25	2462120	2506588	1514892.13	1637817.38	1207498.63
ATP C13-label-5	79870568	31745366	61395196	13236188	25612178	22944446	40666552	33679716	36113332	21828856	22067502	15654120
ATP C13-label-6	22980750	8887143	18449166	1470098.13	2803722.75	2257183.25	7255116	5615974	5793984.5	3007828	3011339.75	2594087
ATP C13-label-7	9466164	3702577.75	6985518.5	566408.69	1079989.38	874677.81	2500020.75	1859150.88	1971858.5	1119635.63	1230162.63	927089.94
ATP C13-label-8	2141865.75	867411.56	1850003	47318.72	98062.52	85407.23	338460.09	354109.34	279931.59	115143.59	159100.67	113815.76
ATP C13-label-9	234093.67	102859.78	222957.58	0	0	0	0	0	0	0	0	0
Aconitate C12 PARENT	414796.66	481034.88	345280.09	168427.27	228158.36	170405.05	167161.88	105882.68	215152.77	113622.84	129734.34	179428.73
Aconitate C13-label-1	27243.23	32421	41561.35	20575.42	0	15361.82	0	0	0	0	0	0
Aconitate C13-label-2	259907.98	435267	319638.19	146448.58	102966.94	153367.33	165725.22	162007.48	123979.87	107851.31	61519.22	111613.22
Aconitate C13-label-3	109818.69	102603.54	96628.84	25397.56	14506.27	13030.69	0	10723.37	38814.33	0	63212.04	46723.56
Aconitate C13-label-4	130233.19	156686.23	146195.7	13646.69	0	0	60189.35	43620.88	36975.16	81016.64	66939.31	76689.11
Aconitate C13-label-5	118702.03	110503.01	73278.63	14139.13	0	0	44313.75	14836.96	26195.77	10229.26	14002.94	10284.36
Argininosuccinate C12 PARENT	198076.98	173061.84	188232.48	111347.48	87348.92	79032.54	127622.5	82463.34	91032.94	71956.63	105912.82	13011.21
Argininosuccinate C13-label-1	23211.55	7299.82	16833.73	0	8560.87	0	12341.16	0	0	0	0	0
Argininosuccinate C13-label-2	81060.88	56620.79	29442.23	12185.21	0	11184.33	0	18695.3	9389.12	10374.01	8222.18	0
Argininosuccinate C13-label-3	37805.65	17116.3	13536.39	0	0	0	9583.39	7363.93	8006.17	0	0	0
Asparagine C12 PARENT	3845960	3863525	3698776.75	2117227	2251708.25	2015586.63	2069609.88	1997178	1819017.38	1440723.38	1770708.63	1181337.63
Asparagine C13-label-1	271209.94	321132.72	265317.09	42111.32	46581.01	72064.65	219698.05	99064.5	67428.46	76421.3	116455.28	99486.85
Asparagine C13-label-2	883141.94	744585.5	710353.19	231051.27	210398.14	207232.55	329528.72	336708.59	181459.69	0	278840.5	259254.17
Asparagine C13-label-3	606972.44	584188.94	687758.31	125742.65	252788.08	155163.61	360742.66	352759.38	99876.63	172552.08	278930.88	194065.95
Asparagine C13-label-4	177669.2	237820.8	206385.98	0	84313.07	0	27227.21	45562.92	0	0	0	17601.49
Aspartate C12 PARENT	44551344	37576000	42879108	29493126	32353586	29074874	31415210	30243306	25508678	14211439	18906358	16502779
Aspartate C13-label-1	4916079.5	4239496	4441775.5	3142970	3462916	2979558.25	3187446.75	2777023.5	2191127.25	1174141.63	1825404.88	1406517
Aspartate C13-label-2	14119911	11384273	12717327	7886000.5	8172762.5	7166236	8445514	7807398	6612092.5	3854400	4830907.5	4222750.5
Aspartate C13-label-3	10689326	8475822	9757892	5427841	5447669	4845264	6565576	6319025.5	5383559	3292576.25	3998801	3440435
Aspartate C13-label-4	4375247.5	3645224.25	3993850.25	1898232.38	1928529.63	1662279	2779913	2513528.75	1765068.38	1270335.38	1552632.88	1310210.63
Aspartate Std	2763097	2514739	2753274	2126058	2629186	2290261.25	2196383.75	2427972	1898924.5	2312182.25	2761569	2186591.25
CDP C12 PARENT	443855.59	204756.25	590775.31	1054328.25	1794797.63	1701097	943117.44	851053.19	802139	606178.13	567108	529138
CDP C13-label-4	90374.63	10614.54	70848.35	0	13935.84	0	40995.59	35812.3	40443.67	25962.02	13500.95	10509.4
CDP C13-label-5	1267400	465300.97	1321851.88	229901.7	388729.41	293655.13	990132.31	878044	954862.5	549068.69	585423.06	481450.66
CDP C13-label-6	288090.38	72210.96	257087.58	13127.01	14177.7	30628.43	234909.88	162350.98	185181.58	36618.91	109088.3	73977.88
CDP C13-label-7	439871.16	148298.16	412820.25	15147.17	30060.71	63078.32	233281.14	213666.88	260051.42	135928.98	146384.2	144961.81
CDP C13-label-8	240444.52	41659.97	245847.39	0	17430.52	0	127687.77	144780.88	76038.89	76232.16	71384.8	53829.33
CDP-ethanolamine C12 PARENT	400832.13	352781.97	429385.66	1137347.63	1075901.63	1092354.13	403569.72	412868.91	394590.84	392990.66	511129	274433.09
CDP-ethanolamine C13-label-5	634282.5	643463.44	865606.75	207419.19	140753.86	152574.86	367014	363904.25	356810.66	335609.84	470165.53	241429.61
CDP-ethanolamine C13-label-6	160386.11	150852.39	221105.73	10375.1	11677.77	13457.83	72943.59	62616.84	53655.11	44513.2	71732.18	26128.91
CDP-ethanolamine C13-label-7	186947.5	229144.13	256834.45	12166.53	12261.51	12300.93	50061.39	80854.38	109718.88	76961.34	78567.29	43420.21
CDP-ethanolamine C13-label-8	67665.08	112919.2	106727.65	11125.15	0	0	30004.15	21848.15	44933.68	25569.84	28404.62	10292.13
CMP C12 PARENT	26330.58	18611.07	31795.53	237192.88	235731.58	301379.66	122631.13	126300.21	203801.45	170152.47	122959.3	43229.16
CMP C13-label-5	100425.56	48325.63	244638.31	16866.71	40150.24	26164.82	63357.75	181394.23	72216.99	48646.08	90741.4	119319.77
CTP C12 PARENT	1000415.44	437417.78	863679.5	1492473	1915376.5	2056445	1067379.88	912870.69	927732.69	655898.63	676035.25	431682.59
CTP C13-label-1	87376.48	38186.64	56451.27	120202.97	174654.67	221488.92	64326.12	24011.31	45403.86	32008.79	21752.14	18990.2
CTP C13-label-4	235112.83	60320.92	120216.5	0	0	12767.74	39079.83	38330.07	41803.36	11941.83	15795.72	0
CTP C13-label-5	2218029.25	1032121.5	2038105.63	259474	357611.53	461053.53	1015471.94	953423.5	903842.44	707772.56	686353.69	481970.63
CTP C13-label-6	666500.94	270281.5	528798.75	19036.51	42494.76	75438.99	207618.7	179642.58	168683.52	151833.66	119449.07	87141.38
CTP C13-label-7	798586.25	299846.28	590861.44	57276.27	123719.71	110112.44	288549.09	211279.38	228748.42	156114.67	209361.67	87799.32
CTP C13-label-8	386863.5	159895.89	300428.5	10842.68	15387.27	20272.59	98160.07	135068.88	124230.68	75979.49	99538.03	41596.38
Carbamoylaspartate C12 PARENT	1725782.63	1702162	1309542.38	1030559.31	1200079.38	978287.31	6063194	5149764.5	6535228	7764358	7548153.5	5288914.5
Carbamoylaspartate C13-label-1	40432.83	82166	64536.32	9616.78	3082.59	31989.46	363565	295763.41	319038.84	449983.28	417902.47	325870.91
Carbamoylaspartate C13-label-2	0	0	0	0	0	0	53258.37	40975.01	0	99277.91	112248.55	32400.41
Carbamoylaspartate C13-label-3	569208.75	547509.06	431171.63	527940.88	504229	376963.28	2193417	1897010.88	2608825	3220835.25	3013485	2054819.88
Carbamoylaspartate C13-label-4	0	0	0	0	0	0	70770.47	36647.76	53724.84	118678.94	31579.81	62461.11
Citrate C12 PARENT	63586740	57264596	61329012	33188010	35097356	29340448	37253616	30273274	32573522	25056986	28286296	21539906
Citrate C13-label-1	9289405	8499889	9089076	5200543.5	5095692.5	4221601.5	4988259.5	4375348	4622435.5	3308482.75	3776234	2907446.75
Citrate C13-label-2	67282888	55129332	71152552	26170976	27069120	25532650	41615100	35465376	34818176	24280314	27700134	22743064
Citrate C13-label-3	17170180	14376099	16778594	7044416.5	7098047.5	6330354	9644816	8340648	8084892.5	6081088	6696017.5	5375421.5
Citrate C13-label-4	26790666	21460810	25656754	8913851	8916099	7954353	16114599	13435983	12984575	9266797	10089387	8377765
Citrate C13-label-5	18806588	15499487	17470716	5268895.5	5565205.5	5167382.5	11417523	9406854	9314076	6938340.5	7700541.5	6247389.5
Citrate C13-label-6	6367918.5	4825869.5	6064538.5	1519860.88	1460975.38	1383747.88	4362018	3358231.75	3264516	2117703.5	2274706.75	1931750
Dihydroorotate C12 PARENT	44707.07	63091.16	55919.19	0	30209.54	0	359420	225907.45	118109.98	194533.8	362305.66	165723.7
Folate C12 PARENT	206647.02	245195.8	367146.88	314748.81	427319.16	422321.97	276300.88	230858.8	407079.34	312987.41	214046.2	268307.59
Fructose-1_6-bisphosphate C12 PARENT	43574.37	86377.66	124008.54	20935.88	16654.78	37396.62	62717.16	36247.19	26324.98	0	16440.35	14966.54
Fructose-1_6-bisphosphate C13-label-5	235389.94	385382.66	336373.59	126243.56	68353.23	49416.13	72048.93	71757.24	41448.89	14843.63	10531.49	15022.47
Fructose-1_6-bisphosphate C13-label-6	2652911.75	4246686	4588125.5	2085277	1131677	877731.81	1261586.63	1085798.63	857099	538124.69	661284.31	467458.16
GDP C12 PARENT	252064.67	109740.88	335673	224397.14	412682.09	385727.16	352052.75	311246.53	300984.13	227185.8	246327.7	165889.72
GDP C13-label-4	165284.55	82094.91	168022.11	58260.47	90983.26	71944.99	150815.98	110893.13	103172	49282.73	109584.81	45907.83
GDP C13-label-5	1985280	874497.75	2732563.75	1204510.63	1758310.13	1920509.38	2687821.25	2236714.75	2006837.88	1186253.88	1394649.63	1008432.69
GDP C13-label-6	696973.19	258806.7	801222.5	120852.32	231159.83	200461.38	456636.38	393974.59	276573.97	163685.5	142331.83	126504.95
GDP C13-label-7	314281.97	98871.55	325498.91	20615.92	36172.04	16107.02	147697.48	109162.87	77201.69	43118.39	24247.32	29319.03
GDP C13-label-8	32403.83	11467.18	55308.13	0	0	0	0	0	13585.55	0	0	0
GMP C12 PARENT	74537.05	9538.75	135168	83901.79	177353.05	175797.17	119519.32	172631.81	177982.14	100493.25	174605.73	45361.67
GMP C13-label-4	23072.14	0	39580.02	24685.17	18369.15	13026.25	59887.56	99088	105629.31	39702.78	50402.41	0
GMP C13-label-5	643787.56	281877.28	1000039.06	491827.38	684179.13	805935.69	1165891.13	1080914.88	1108295.38	479908.28	867328.31	604616.88
GMP C13-label-6	238559.77	38813.64	366749.78	33509.75	46853.18	54269.63	206633.75	137886.5	156812.56	40128.29	74576.32	68593.15
GMP C13-label-7	27984.57	10877.85	140124.53	0	0	0	31113.96	34198.33	0	11638.86	0	0
GMP C13-label-8	0	0	0	0	0	0	0	0	0	0	0	0
GTP C12 PARENT	5195705.5	1082282.63	3440158	1666455	3642432.75	3304036.75	3383859.25	2946756	3581279	2666584.75	2997578.75	1612632.63
GTP C13-label-1	662886.81	102651.1	421908.25	172454.75	557599.69	209039.81	522502.28	173799.14	439884.38	110120.05	130829.4	50381.2
GTP C13-label-2	433341.28	65922.99	380191.5	93132.57	265576	349258.78	352030.88	273646.94	290622.69	144868.13	204544.36	156720.14
GTP C13-label-3	655646.56	32006	423322.78	89400.85	272761.91	241988.05	262957.06	292321.16	395109.91	287316.41	347870.25	123939.98
GTP C13-label-4	2674879.75	600489.06	1702603.88	610483.38	1223158.75	1309864.63	1887783.88	1566461.88	1697854.5	969360.44	1053687.38	591317
GTP C13-label-5	40719756	9822527	27348698	6616208.5	15806284	13098568	24232280	19403746	25297002	16107423	15302635	10542123
GTP C13-label-6	13639617	3003921.25	8536328	695608.94	1695722.88	1704733.38	4569993.5	3144692.75	3926129.25	1969590.63	2105954.75	1770143.13
GTP C13-label-7	4852789.5	1321491	3597918.75	257602.44	751731.19	534261.81	1706726	1179584.38	1609210.38	905657.69	931792	554774.56
GTP C13-label-8	1350808.63	192588.3	829827.81	0	0	0	190956.73	49228.58	212741.86	0	42879.33	25988.95
GTP C13-label-9	112236.75	0	0	0	0	0	0	0	0	0	0	0
Gluconic acid C12 PARENT	1218941.13	1203043.5	874035.81	269240.09	362974.66	224806.11	607166.75	604694.06	754755.31	565735.44	725795	556072.06
Glucose C12 PARENT	0	158864.7	159852.52	350995.41	256807.63	0	241614	191209.83	311666	199969.8	290532.91	295091.28
Glucose C13-label-5	724841.69	1171284.63	1146762.13	1424776	1281265.75	1590281.88	1207809	1184110.63	1866762.63	1154285.5	1323442.63	1314085.88
Glucose C13-label-6	9033571	16424749	13845664	19173866	16930098	20630610	15665520	15716071	23005018	15053315	16469913	16730668
Glucuronic acid C12 PARENT	0	0	8509.57	0	0	10689.75	8938.08	8902.97	0	0	16187.98	0
Glucuronic acid C13-label-6	305951.03	254107.41	304683.28	70325.52	55822.34	32694.07	37887.68	42331.53	54790.09	18777.11	41542.52	16192.18
Glutamate C12 PARENT	392894688	366929760	349592928	246169984	253944512	236140848	317148928	251839024	274412512	222170752	274415552	186542288
Glutamate C13-label-1	34593300	32669386	31315290	21864854	22699062	20899570	26156258	21957142	21919606	17574626	21841978	15011637
Glutamate C13-label-2	145316608	135814688	128845656	71782128	72924992	67901800	97968512	77939704	80257864	60288260	76208976	53695940
Glutamate C13-label-3	49434908	45089876	41935760	21857132	22622264	20199330	32288310	25220422	25056190	19425830	24108694	16890676
Glutamate C13-label-4	60961156	56537428	53102700	24193296	23944246	22382298	41998756	33381074	32760480	25118606	29791758	21363682
Glutamate C13-label-5	37795072	35850348	32611904	13374661	13150403	11113308	25826978	19871538	19832806	15969320	19646538	13413069
Glutamate Std	1943089.5	2078899.63	2276012.75	2232647.75	2176246.75	2152630.75	2095476.38	1924080.88	1857900	1843456.38	2523053	2050598.5
Glutamine C12 PARENT	1046260480	994364096	1025578944	647747968	595262912	609259008	665514240	705332416	711983552	478530784	598019648	402877664
Glutamine C13-label-1	59490272	57977524	58688860	37319388	33508278	34825604	37798452	39933580	40413652	26637016	34690992	22685184
Glutamine C13-label-2	9968335	9812007	10571613	4116858.25	4041392	3945880.25	4336579.5	4650063.5	4090822	2581011.25	2659930.75	2370154
Glutamine C13-label-3	3075509.25	2819923.75	2995403.75	1243898.13	874556.06	1241077.88	1075963.13	1476758	873463.81	438106.28	944437.69	599520.56
Glutamine C13-label-4	4325520.5	3441355	3433566	1009370.44	1099416.13	1149092.38	1415616	1294939.38	1241423	817767.25	1044475.69	751492.25
Glutamine C13-label-5	2842924.75	2278500.75	2198404.5	684033.81	454987.25	397013.47	910263.31	1035426.06	492671.63	621669.56	674337.81	563286.81
Glycerol-3-phosphate C12 PARENT	756896.5	669001	411925.75	618261	773526.94	622442.13	538570.5	414205.78	953714.5	984931.81	879658.69	631700.88
Glycerol-3-phosphate C13-label-3	1607025	1128381.5	603141.31	577936.94	985351.69	903685.06	862972.19	756792.19	1444790.5	1763240.38	1569042.63	979112.25
Hexose phosphate C12 PARENT	466036.84	559736.5	444069.25	185159.39	65255.27	147842.2	296501.97	247283.75	332376.72	258762.17	240792.38	141350.3
Hexose phosphate C13-label-5	93316.13	25591.2	128662.35	51121.88	27286.96	45153.77	16405.47	44658.16	77007.98	17651.05	46629.48	24123.35
Hexose phosphate C13-label-6	1065567.38	1009163.63	1358915.63	831964.25	795872.31	878451	761522.31	645380.5	815131.81	715964.75	565905.5	579100.81
Inosine monophosphate C12 PARENT	0	11259.8	16784.47	142466.02	75237.49	133485.25	52824.27	86758.18	66368.32	33712.68	95853.6	19634.39
Inosine monophosphate C13-label-4	12829.95	0	11805.2	0	10757.8	12712.81	0	0	22721.86	0	25471.21	0
Inosine monophosphate C13-label-5	191190.31	193603.56	346701.03	328647.47	356758.47	305440.78	308668.75	352289.78	305123.72	290708.78	344793.91	196064
Inosine monophosphate C13-label-6	0	0	0	0	0	0	0	0	0	0	0	0
Inosine monophosphate C13-label-7	0	0	0	0	0	0	0	0	0	0	0	0
Inosine monophosphate C13-label-8	0	0	0	0	0	0	0	0	0	0	0	0
Inosine monophosphate C13-label-9	0	0	0	0	0	0	0	0	0	0	0	0
Inositol C12 PARENT	235729776	505939200	484320352	93612224	182762192	97202776	309038048	246942336	109214104	97045112	204821872	153725808
Lactate C12 PARENT	2267179.25	2589860.5	2689036.75	1867722	1993148.5	1502929.88	2194884.5	1485429	1906434.63	1168294.63	1906878	1071811.5
Lactate C13-label-1	62189.69	220006.75	214771.56	0	0	0	69227.02	0	0	0	0	0
Lactate C13-label-2	966914.19	920711.75	1209385.13	158711.8	117623.91	0	317982.69	229227.5	478636.25	101985.7	337520.5	138573.22
Lactate C13-label-3	18777432	22775638	25131248	5015226	4323531.5	5827696	7562240	6240209.5	9346229	4598810	6973332	5510252
Malate C12 PARENT	61132324	57204800	52380788	35193972	34374988	29190064	43658908	41738924	37766820	28656102	29486326	23625974
Malate C13-label-1	6270956.5	5419741.5	4879102	3065107.75	3074879.75	2658161.75	3699670.25	3225732.75	2983634.75	2094850.25	2477131	1949170.5
Malate C13-label-2	18272622	16585281	14736275	8388144	8301389.5	6953568	11628659	10526348	9190897	6484294.5	7030583.5	6416218
Malate C13-label-3	14003809	12352244	11409992	5414952.5	5339005.5	4796017.5	8765995	8003986	7235996.5	5507612.5	5742965.5	4611813.5
Malate C13-label-4	6249213.5	5282961.5	4896301.5	2216132	2132963	1776473.63	3810942.75	3402742.75	3118161.25	2443032.75	2427037.25	1797862
N-Acetylglucosamine-6-phosphate C12 PARENT	194118.7	198122.17	189058.11	66985.26	120760.04	52103.14	190474.75	106776.21	140683.08	103397.13	75222.8	40409.38
N-Acetylglucosamine-6-phosphate C13-label-6	261444.38	201705.42	298543.16	69845.5	120543.88	162915.86	128516.98	135814	125784.63	65898.92	109361.88	98112.49
N-Acetylglucosamine-6-phosphate C13-label-8	328321.81	290646.91	274335.38	81330.8	111344.26	82568.2	229076.2	230718.42	130780.38	128319.2	70164.57	102532.73
Orotate C12 PARENT	751132	141922.52	1209383.13	160291.11	361168.13	488035.47	1867898.88	1660757.13	972020.75	644195.94	1406579.38	547391.06
Orotate C13-label-1	15592.81	0	65391.63	0	0	31984.71	101328.75	108519.73	58824.14	41796.73	51363.99	19162.2
Orotate C13-label-2	26763.74	0	30638.39	0	10756.63	0	80669.63	50407.55	0	15037.02	41174.66	0
Orotate C13-label-3	232779.39	13953.61	428027.41	52740.93	121525.59	190729.63	652865.81	654600.81	341171.56	289397.25	564794.81	273888.69
Orotate C13-label-4	0	0	0	0	0	0	10341.95	11217.98	14446.59	0	10079.85	0
Orotidine C12 PARENT	5046108.5	4361573	4347856.5	1387136.63	1371215.5	1324441.25	1855847.63	1719172.88	1678985	1187684.38	1369661	955605.56
Orotidine C13-label-1	501646.25	467280.22	420831.09	165090.2	188335.7	148467.33	192680.25	169874.45	184919.52	109935.43	159262.5	86391.39
Orotidine C13-label-4	378931.41	293993.5	255029.5	18140.13	30269.13	48073.39	96895.59	77645.52	79880.32	50886.67	37229.49	26795.05
Orotidine C13-label-5	5001672	4152565.25	4106058	606803.75	650752.94	589010.38	1449420.38	1250434.88	1213768.88	757039.25	830501.69	721831.69
Orotidine C13-label-6	644373.06	584137.5	505397.5	51288.24	57304.91	52943.09	163868.27	122136.84	139102.66	56748.18	92844.85	65974.55
Orotidine C13-label-7	1373721.13	1087740.13	1099186.13	170188.38	161332.55	112771.15	277155.13	276999.66	272491.41	156501.14	200499.13	149523.89
Orotidine C13-label-8	1195533.63	903106.44	956824.94	134618.31	128307.04	118107.5	275812.38	253256.3	256688.13	148819.11	205075.89	121811.26
Orotidine C13-label-9	359306.84	312399.16	330132.84	0	0	0	0	0	0	0	0	0
Palmitate C12 PARENT	11226720	47067562	24184452	34397190	23337256	20034102	33886178	26624998	43735522	10670246	17341264	5052482
Pantothenate C12 PARENT	33445318	26562086	30492488	11142606	11822825	10463599	16789604	17858336	16557492	11092981	21405478	16083771
Phosphate C12 PARENT	123222752	154611696	143226480	155264080	147218496	149405120	145253808	166255104	172437584	153075120	127946072	123151752
Phosphoenolpyruvate C12 PARENT	24289.31	89515.38	37936.56	43653.22	0	22763.03	0	0	12618.75	40524.25	15192.65	10796.79
Phosphoenolpyruvate C13-label-3	495248.59	32659.28	15671.36	10534.85	473899.25	317261	538407.69	446624.97	466714.59	429549.97	646971.13	343887.84
Phosphoethanolamine C12 PARENT	1298162.63	1316054.38	1287246.13	2261179.75	2307060.75	2309383	785315.81	775652.5	1012559.19	1322971.38	1414283.63	680327.75
Phosphoribosyl pyrophosphate C12 PARENT	0	0	0	0	0	0	0	0	0	0	0	0
Phosphoribosyl pyrophosphate C13-label-4	56888.41	13646.86	58312.94	0	0	23175.56	36170.73	21750.01	28125.57	0	0	0
Phosphoribosyl pyrophosphate C13-label-5	897723	301576.47	1004867.56	161814.52	535026.06	360156.97	672649.75	510911.78	687180.69	312542.41	167528.27	300881.47
Phosphoserine C12 PARENT	465639.78	418254.91	427208.41	126696.38	118779.09	129110.62	21553.11	58140.93	60467.18	17770.39	0	0
Pyrophosphate C12 PARENT	120433.73	152749.73	533860.06	146840.44	215799.42	137340.23	236942.7	300511.34	248511.27	128691.85	153858.44	64968.26
Ribose-5-phosphate C12 PARENT	0	0	0	0	0	10419.89	19242.71	17616.11	0	0	0	0
Ribose-5-phosphate C13-label-5	571060.19	270728.72	339810.78	144947.34	659412.19	722108.31	616213.81	541403.19	699001.25	535874.44	494761.47	586387
Sedoheptulose-7-phosphate C12 PARENT	0	24641.69	0	66247.66	20302.64	66954.87	39611.07	64449.72	87547.27	21365.98	86042.3	49606.05
Sedoheptulose-7-phosphate C13-label-7	451497.66	456409.41	618244.19	198053.95	176698.95	172544.19	194490.63	226283.61	317889.22	347009.41	327246.22	336492.22
Serine C12 PARENT	59427180	66964724	63171644	41578976	40191240	39301788	42093852	48747084	41819824	29083658	36000220	25066842
Serine C13-label-1	9602255	11004005	10184220	3228520.75	3140786.25	2974398.75	4356538	4388088.5	3894409.75	1947713.13	2705598.25	2060156.13
Serine C13-label-2	4952674.5	5143512	5061756.5	1352398.63	1453867.13	1166082.5	1952625	1472557.5	1440766.13	951029.25	952518.5	849996.25
Serine C13-label-3	7540298.5	7974880	7868048	1468053.88	1428020.38	1520722.5	2038405.88	2363539.5	1658218.38	1000128	1018755.81	962101.5
Serine Std	2290170.75	2348124.25	1995210.38	1907510.38	1735964.5	1932889.5	1504896.38	1701491.63	1330540.13	1488711.38	1512846.63	1560912.88
Sorbitol C12 PARENT	87459104	80337864	56659924	59391340	48881088	40041236	22896586	56905244	30306746	38014872	19426234	22357788
Sorbitol C13-label-1	5623803.5	5069808.5	3829838	3762925.25	3261723.75	2777895	1550508.5	3710269	2089097.13	2569925.5	1212465.5	1515941.63
Sorbitol C13-label-6	621946.94	762761.69	770834.75	211180.98	321278.75	223485.39	669428.25	637357.81	543049.31	355881.72	651174	363458
Succinate C12 PARENT	2581550	2687250	2421455.75	1904278.13	1686553.38	2031119.13	2457521	2089261.13	3081847.25	2397056.75	2518129.25	1907035.38
Succinate C13-label-1	99379.44	155869.08	188294.75	95847.32	108663.23	77545.34	92834.7	97000.91	117441.98	122493.23	84276.7	46276.47
Succinate C13-label-2	610910.56	796036.06	527753.69	321027.09	256537.89	174004.44	423854.38	284953.03	448638.13	300312.88	291250.91	225610.17
Succinate C13-label-3	355596.13	362294.59	241509.3	113595.55	104862.63	96917.51	147811.86	224831.11	270249.03	149232.77	180628.8	106256.82
Succinate C13-label-4	172069.5	257439.33	174624.52	78810.5	44811	55669.05	116942.15	136542.78	147983.2	81897.65	124289.94	81822.57
Thiosulfate C12 PARENT	755124.19	693194.94	897129	802143.75	1173843	1318127.88	892651.81	1263040	1147395.63	895255.69	1177668.88	864861.81
UDP C12 PARENT	498910.81	176582.95	494128.69	1470709.63	2334979.75	2178486	1026664.38	988563.31	793128.31	635526.44	663407.75	485242.19
UDP C13-label-1	56425.36	0	71116.09	163826.05	202991.5	239214.75	71073.27	69049.73	65725.45	88355.1	30542.56	32300.17
UDP C13-label-2	31396.13	0	50808.68	24574.64	69985.77	57819.75	37725.58	17893.28	14642.96	20516.45	23032.8	0
UDP C13-label-3	77702.59	16786.64	47104.91	17863.27	20367.01	48376.61	75490.34	76000.24	36214.95	20130.49	16673.32	17851.29
UDP C13-label-4	332784.28	149551.36	402365.38	89571.19	174694.81	137138.5	362156.03	287559.63	270224.97	150477.42	156795.58	145704.25
UDP C13-label-5	4948759.5	1955940.5	4620840.5	1728897.63	2250318	2215677.25	4252573.5	4078413.25	3229588	2496451	2305388.25	1938781.88
UDP C13-label-6	1167217.25	407617.09	1195821.88	335300.72	458264.09	420611.34	887161.19	808910.94	613519.94	489835.72	533569.81	434098.63
UDP C13-label-7	1667121.5	536095.44	1419640.88	415061.66	621723.31	531994.75	1220707.38	1165907.63	897267.06	722311.44	688577.81	500154.66
UDP C13-label-8	861158	380965.53	783431.69	209254.61	292489.03	255280.98	711078.31	614988.06	433859.25	380530.91	374326.91	277254.63
UDP-N-acetylglucosamine C12 PARENT	570132.81	579665.25	743309.25	1012610.56	909700.19	892014.75	670470.94	726629.06	846293.31	664890.56	685752.69	585050.75
UDP-N-acetylglucosamine C13-label-1	70456.51	120835.28	49031.06	146166.77	179974.98	183606.36	122942.27	113930.34	185736.17	48835.35	75788.26	99252.99
UDP-N-acetylglucosamine C13-label-2	84817.6	122153.54	121144.7	198097.88	152633.94	141196.88	212222.13	147344.3	235704.44	136810.45	117170.9	115396.04
UDP-N-acetylglucosamine C13-label-4	254525.14	226292.02	180349.98	104180.34	33004.13	22993.5	121821.52	121874.15	193361.17	192370.61	174557.14	100890.75
UDP-N-acetylglucosamine C13-label-5	2718244.25	3258358	2677438.25	812404.75	831094.44	880734.25	1675780.88	1919211.63	2169395.25	1914899.5	1756245.63	1342362.63
UDP-N-acetylglucosamine C13-label-6	1569787.63	1799687.63	1797246.38	1963176.63	2329768	1906917.88	1314837.13	1800156	1880966.63	1438715.38	1275913.13	1013001.5
UDP-N-acetylglucosamine C13-label-7	1456791	1713743.5	1346276	681399.5	671804.81	830400.06	1031445	1195692.13	1215677.88	1150189	1033007	800957.81
UDP-N-acetylglucosamine C13-label-8	1498622.13	1663386	1466759.5	1918792	1890673	1975489.88	1468555.88	1829413.38	1462758.88	1238705.75	1098864	928846.56
UDP-N-acetylglucosamine C13-label-9	579236.5	700183.31	594097.06	290467.41	318838.34	374255.34	442501.28	553958.38	423900.5	312379.63	378136.97	302422.38
UDP-N-acetylglucosamine C13-label-10	1160037.13	1394096	1265824.88	387617.41	374090.66	320261.81	766099.69	832854	766972.56	585413.56	551964.44	530357.44
UDP-N-acetylglucosamine C13-label-11	5833125.5	6761128	6206831.5	1952581.88	2022300.5	1987253.38	3484690.25	4016332.75	4013291	2959027.75	2469686	2117927.75
UDP-N-acetylglucosamine C13-label-12	2934874	2753378.5	2649209.5	736934.25	713761.19	646716.94	1565573.63	1603816.13	1442185.5	1007573	966417.25	920388.56
UDP-N-acetylglucosamine C13-label-13	8040130.5	8936011	7507229.5	1830781.88	1906986.38	1676285.5	4104557.75	4349434.5	4222552	2862985.25	2629432	2396543.25
UDP-N-acetylglucosamine C13-label-14	2978183.25	3005975.75	3177713.25	627809.63	533331.5	673422.81	1633693.38	1661953.38	1553090.38	1071207	993341.31	857211.94
UDP-N-acetylglucosamine C13-label-15	2680443.75	2923460	2730916.75	571379.06	520948.75	426863.72	1521093.88	1673380.13	1318267	848612.5	908501.5	789317.25
UDP-N-acetylglucosamine C13-label-16	1556933.5	1488990.13	1483037	316773.47	200638.39	215616.27	694292	603932.19	690905.06	524442.13	365750	396628
UMP C12 PARENT	18924.45	32626.67	140907.08	386851.25	281694.97	360585.59	111316.25	280825.66	85061.74	88092.4	101675.59	67294.32
UMP C13-label-4	0	27408.04	0	0	0	0	24837.95	30538.17	24738.93	0	12278.64	0
UMP C13-label-5	488589.5	651503.19	1283262.88	387677.72	486985.5	433027.72	782435.06	1142057	796668.25	455499.28	612278.06	384793.72
UMP C13-label-6	56004.91	55243.23	292089.22	0	77826.84	24875.3	143671.69	114945.25	31860.52	29710.19	125553.75	56159.17
UMP C13-label-7	56236.16	58449.43	326700.34	104946.72	59797.24	109927.23	155100.7	222970.08	108621.44	135545.95	72794.54	105607.35
UMP C13-label-8	52998.71	75816.03	171494.88	52706.44	17495.46	0	80437.45	17459.38	89632.63	36932.8	34024.58	71301.26
UTP C12 PARENT	826982.56	537770.25	619676.19	1612658.13	2312967	2251369.25	1035177.5	907821.69	1052910.5	697779.31	618660.56	365824.91
UTP C13-label-1	20921.82	47325.42	41719.52	140984	199979.25	153312.39	94692.9	97346.09	53048.36	41316.98	19141.88	24216.3
UTP C13-label-2	136574.97	35904.57	41971.89	43381.95	62719.44	105968.48	51204.92	63536.8	56264.41	0	18645.19	30107.35
UTP C13-label-3	90224.12	13690.64	84354.68	0	45065.26	39037.25	39660	35930.96	67104.91	13688.97	37487.41	0
UTP C13-label-4	612101.5	301847.88	366195	122661.88	200701	245605.69	257968.08	307407.72	215632.92	197590.08	216474.77	114154.98
UTP C13-label-5	8885002	4285927.5	6540267.5	1700714	2461844.25	2092419.5	4027159	3777780	3998371.25	2834643.25	2478535	1797590.63
UTP C13-label-6	2215934.75	1107477.5	1534510.38	353857.66	541199.56	497685.72	770525.5	892360.69	829362	498073.41	479577.16	390258.91
UTP C13-label-7	2990411.25	1373272.13	1986556.13	448710.13	630756.5	572394.19	1118853	1093643	970930.75	812842.19	760413.56	449892.78
UTP C13-label-8	1688333.88	731154.06	868190	224442.8	297355.69	299527.78	662644.81	514882.22	521807.47	432391.38	378446	267695.5
Xanthine C12 PARENT	29921.41	70062.16	66277.74	216482.73	186655.89	198812.06	77822.85	58367.5	40828	28483.31	29636.29	29002.72
a-Ketoglutarate C12 PARENT	305460.09	3103467	2301340.75	968396.25	151968.28	116327.05	235867.67	297096.41	151281.92	137585.2	165025.31	102139.19
a-Ketoglutarate C13-label-2	0	1061783.83	709171.7	156675.08	0	0	0	0	0	0	0	0
a-Ketoglutarate C13-label-3	0	370746.91	248869.55	26432.04	0	0	0	0	0	0	0	0
a-Ketoglutarate C13-label-4	0	602750.5	290817.78	0	0	0	0	0	0	0	0	0
a-Ketoglutarate C13-label-5	0	349427.38	242707.67	0	0	0	0	0	0	0	0	0
MS_METABOLITE_DATA_END
#METABOLITES
METABOLITES_START
metabolite_name	KEGG.ID
2-HG C12 PARENT	C02630
2-HG C13-label-2	C02630
2-HG C13-label-3	C02630
2-HG C13-label-4	C02630
2-HG C13-label-5	C02630
3-Phosphoglycerate C12 PARENT	C00197
3-Phosphoglycerate C13-label-3	C00197
4-Hydroxyphenyllactate C12 PARENT	C03672
5-Oxoproline C12 PARENT	C01879
5-Oxoproline C13-label-1	C01879
5-Oxoproline C13-label-2	C01879
5-Oxoproline C13-label-4	C01879
5-Oxoproline C13-label-5	C01879
6-Phosphogluconate C12 PARENT	C00345
6-Phosphogluconate C13-label-6	C00345
ADP C12 PARENT	C00008
ADP C13-label-1	C00008
ADP C13-label-2	C00008
ADP C13-label-3	C00008
ADP C13-label-4	C00008
ADP C13-label-5	C00008
ADP C13-label-6	C00008
ADP C13-label-7	C00008
ADP C13-label-8	C00008
ADP C13-label-9	C00008
ATP C12 PARENT	C00002
ATP C13-label-1	C00002
ATP C13-label-2	C00002
ATP C13-label-3	C00002
ATP C13-label-4	C00002
ATP C13-label-5	C00002
ATP C13-label-6	C00002
ATP C13-label-7	C00002
ATP C13-label-8	C00002
ATP C13-label-9	C00002
Aconitate C12 PARENT	C00417
Aconitate C13-label-1	C00417
Aconitate C13-label-2	C00417
Aconitate C13-label-3	C00417
Aconitate C13-label-4	C00417
Aconitate C13-label-5	C00417
Argininosuccinate C12 PARENT	C03406
Argininosuccinate C13-label-1	C03406
Argininosuccinate C13-label-2	C03406
Argininosuccinate C13-label-3	C03406
Asparagine C12 PARENT	C00152
Asparagine C13-label-1	C00152
Asparagine C13-label-2	C00152
Asparagine C13-label-3	C00152
Asparagine C13-label-4	C00152
Aspartate C12 PARENT	C00049
Aspartate C13-label-1	C00049
Aspartate C13-label-2	C00049
Aspartate C13-label-3	C00049
Aspartate C13-label-4	C00049
Aspartate Std	C00049
CDP C12 PARENT	C00112
CDP C13-label-4	C00112
CDP C13-label-5	C00112
CDP C13-label-6	C00112
CDP C13-label-7	C00112
CDP C13-label-8	C00112
CDP-ethanolamine C12 PARENT	C00570
CDP-ethanolamine C13-label-5	C00570
CDP-ethanolamine C13-label-6	C00570
CDP-ethanolamine C13-label-7	C00570
CDP-ethanolamine C13-label-8	C00570
CMP C12 PARENT	C00055
CMP C13-label-5	C00055
CTP C12 PARENT	C00063
CTP C13-label-1	C00063
CTP C13-label-4	C00063
CTP C13-label-5	C00063
CTP C13-label-6	C00063
CTP C13-label-7	C00063
CTP C13-label-8	C00063
Carbamoylaspartate C12 PARENT	C00438
Carbamoylaspartate C13-label-1	C00438
Carbamoylaspartate C13-label-2	C00438
Carbamoylaspartate C13-label-3	C00438
Carbamoylaspartate C13-label-4	C00438
Citrate C12 PARENT	C00158
Citrate C13-label-1	C00158
Citrate C13-label-2	C00158
Citrate C13-label-3	C00158
Citrate C13-label-4	C00158
Citrate C13-label-5	C00158
Citrate C13-label-6	C00158
Dihydroorotate C12 PARENT	C00337
Folate C12 PARENT	C00504
Fructose-1_6-bisphosphate C12 PARENT	C00354
Fructose-1_6-bisphosphate C13-label-5	C00354
Fructose-1_6-bisphosphate C13-label-6	C00354
GDP C12 PARENT	C00035
GDP C13-label-4	C00035
GDP C13-label-5	C00035
GDP C13-label-6	C00035
GDP C13-label-7	C00035
GDP C13-label-8	C00035
GMP C12 PARENT	C00144
GMP C13-label-4	C00144
GMP C13-label-5	C00144
GMP C13-label-6	C00144
GMP C13-label-7	C00144
GMP C13-label-8	C00144
GTP C12 PARENT	C00044
GTP C13-label-1	C00044
GTP C13-label-2	C00044
GTP C13-label-3	C00044
GTP C13-label-4	C00044
GTP C13-label-5	C00044
GTP C13-label-6	C00044
GTP C13-label-7	C00044
GTP C13-label-8	C00044
GTP C13-label-9	C00044
Gluconic acid C12 PARENT	C00191
Glucose C12 PARENT	C00031
Glucose C13-label-5	C00031
Glucose C13-label-6	C00031
Glucuronic acid C12 PARENT	C00191
Glucuronic acid C13-label-6	C00191
Glutamate C12 PARENT	C00025
Glutamate C13-label-1	C00025
Glutamate C13-label-2	C00025
Glutamate C13-label-3	C00025
Glutamate C13-label-4	C00025
Glutamate C13-label-5	C00025
Glutamate Std	C00025
Glutamine C12 PARENT	C00064
Glutamine C13-label-1	C00064
Glutamine C13-label-2	C00064
Glutamine C13-label-3	C00064
Glutamine C13-label-4	C00064
Glutamine C13-label-5	C00064
Glycerol-3-phosphate C12 PARENT	C00093
Glycerol-3-phosphate C13-label-3	C00093
Hexose phosphate C12 PARENT	C00085
Hexose phosphate C13-label-5	C00085
Hexose phosphate C13-label-6	C00085
Inosine monophosphate C12 PARENT	C00130
Inosine monophosphate C13-label-4	C00130
Inosine monophosphate C13-label-5	C00130
Inosine monophosphate C13-label-6	C00130
Inosine monophosphate C13-label-7	C00130
Inosine monophosphate C13-label-8	C00130
Inosine monophosphate C13-label-9	C00130
Inositol C12 PARENT	C00137
Lactate C12 PARENT	C00186
Lactate C13-label-1	C00186
Lactate C13-label-2	C00186
Lactate C13-label-3	C00186
Malate C12 PARENT	C00149
Malate C13-label-1	C00149
Malate C13-label-2	C00149
Malate C13-label-3	C00149
Malate C13-label-4	C00149
N-Acetylglucosamine-6-phosphate C12 PARENT	C00357
N-Acetylglucosamine-6-phosphate C13-label-6	C00357
N-Acetylglucosamine-6-phosphate C13-label-8	C00357
Orotate C12 PARENT	C00295
Orotate C13-label-1	C00295
Orotate C13-label-2	C00295
Orotate C13-label-3	C00295
Orotate C13-label-4	C00295
Orotidine C12 PARENT	X00006
Orotidine C13-label-1	X00006
Orotidine C13-label-4	X00006
Orotidine C13-label-5	X00006
Orotidine C13-label-6	X00006
Orotidine C13-label-7	X00006
Orotidine C13-label-8	X00006
Orotidine C13-label-9	X00006
Palmitate C12 PARENT	C00249
Pantothenate C12 PARENT	C00864
Phosphate C12 PARENT	C00009
Phosphoenolpyruvate C12 PARENT	C00074
Phosphoenolpyruvate C13-label-3	C00075
Phosphoethanolamine C12 PARENT	C00076
Phosphoribosyl pyrophosphate C12 PARENT	C00119
Phosphoribosyl pyrophosphate C13-label-4	C00119
Phosphoribosyl pyrophosphate C13-label-5	C00119
Phosphoserine C12 PARENT	C01005
Pyrophosphate C12 PARENT	C01005
Ribose-5-phosphate C12 PARENT	C00117
Ribose-5-phosphate C13-label-5	C00117
Sedoheptulose-7-phosphate C12 PARENT	C05382
Sedoheptulose-7-phosphate C13-label-7	C05382
Serine C12 PARENT	C00065
Serine C13-label-1	C00065
Serine C13-label-2	C00065
Serine C13-label-3	C00065
Serine Std	C00065
Sorbitol C12 PARENT	C00794
Sorbitol C13-label-1	C00794
Sorbitol C13-label-6	C00794
Succinate C12 PARENT	C00042
Succinate C13-label-1	C00042
Succinate C13-label-2	C00042
Succinate C13-label-3	C00042
Succinate C13-label-4	C00042
Thiosulfate C12 PARENT	C00320
UDP C12 PARENT	C00015
UDP C13-label-1	C00015
UDP C13-label-2	C00015
UDP C13-label-3	C00015
UDP C13-label-4	C00015
UDP C13-label-5	C00015
UDP C13-label-6	C00015
UDP C13-label-7	C00015
UDP C13-label-8	C00015
UDP-N-acetylglucosamine C12 PARENT	C00043
UDP-N-acetylglucosamine C13-label-1	C00043
UDP-N-acetylglucosamine C13-label-2	C00043
UDP-N-acetylglucosamine C13-label-4	C00043
UDP-N-acetylglucosamine C13-label-5	C00043
UDP-N-acetylglucosamine C13-label-6	C00043
UDP-N-acetylglucosamine C13-label-7	C00043
UDP-N-acetylglucosamine C13-label-8	C00043
UDP-N-acetylglucosamine C13-label-9	C00043
UDP-N-acetylglucosamine C13-label-10	C00043
UDP-N-acetylglucosamine C13-label-11	C00043
UDP-N-acetylglucosamine C13-label-12	C00043
UDP-N-acetylglucosamine C13-label-13	C00043
UDP-N-acetylglucosamine C13-label-14	C00043
UDP-N-acetylglucosamine C13-label-15	C00043
UDP-N-acetylglucosamine C13-label-16	C00043
UMP C12 PARENT	C00105
UMP C13-label-4	C00105
UMP C13-label-5	C00105
UMP C13-label-6	C00105
UMP C13-label-7	C00105
UMP C13-label-8	C00105
UTP C12 PARENT	C00075
UTP C13-label-1	C00075
UTP C13-label-2	C00075
UTP C13-label-3	C00075
UTP C13-label-4	C00075
UTP C13-label-5	C00075
UTP C13-label-6	C00075
UTP C13-label-7	C00075
UTP C13-label-8	C00075
Xanthine C12 PARENT	C00385
a-Ketoglutarate C12 PARENT	C00026
a-Ketoglutarate C13-label-2	C00026
a-Ketoglutarate C13-label-3	C00026
a-Ketoglutarate C13-label-4	C00026
a-Ketoglutarate C13-label-5	C00026
METABOLITES_END
#END