#METABOLOMICS WORKBENCH chaoyingzhang_20250508_195604 DATATRACK_ID:5898 STUDY_ID:ST003914 ANALYSIS_ID:AN006427 PROJECT_ID:PR002449
VERSION             	1
CREATED_ON             	May 14, 2025, 12:21 pm
#PROJECT
PR:PROJECT_TITLE                 	Metabolomic profiling of glycogenolysis-derived G1P promotes pentose phosphate
PR:PROJECT_TITLE                 	pathway in CD8+ memory T cell
PR:PROJECT_SUMMARY               	Glucose-6-phosphate (G6P) is a key metabolic molecule that regulates reactive
PR:PROJECT_SUMMARY               	oxygen species (ROS) homeostasis by initiating the pentose phosphate pathway
PR:PROJECT_SUMMARY               	(PPP) to generate NADPH that converts H2O2 to water by providing hydrogen. While
PR:PROJECT_SUMMARY               	both glucose phosphorylation and glycogenolysis result in G6P production, here
PR:PROJECT_SUMMARY               	we show that G6P derived from glycogenolysis, rather than glucose
PR:PROJECT_SUMMARY               	phosphorylation, flows to PPP for ROS clearance in CD8+ memory T (Tm) cells and
PR:PROJECT_SUMMARY               	inflammatory macrophages. Mechanistically, glycogenolysis-produced G1P
PR:PROJECT_SUMMARY               	allosterically induces G6P dehydrogenase (G6PD) binding to glycogen, which
PR:PROJECT_SUMMARY               	together undergo liquid-liquid phase separation (LLPS) and recruit PPP enzymes,
PR:PROJECT_SUMMARY               	resulting in a compartmentalized reaction cascade. Based on mechanistic
PR:PROJECT_SUMMARY               	elucidation, we demonstrated that G1P can act as an antitumor immunotherapeutic
PR:PROJECT_SUMMARY               	agent by modulating memory fitness and maintenance of tumor-reactive CD8+ T
PR:PROJECT_SUMMARY               	cells in mice. These findings revealed an unusual function of glycogen
PR:PROJECT_SUMMARY               	metabolism, which is of paramount importance in the regulation of PPP and redox
PR:PROJECT_SUMMARY               	homeostasis in cells.
PR:INSTITUTE                     	Peking Union Medical College
PR:LAST_NAME                     	Zhang
PR:FIRST_NAME                    	Chaoying
PR:ADDRESS                       	No. 5, Dongdan santiao, Dongcheng District, Beijing, Beijing, Beijing, 100005,
PR:ADDRESS                       	China
PR:EMAIL                         	zhouyaboqq@gmail.com
PR:PHONE                         	860169156464
#STUDY
ST:STUDY_TITLE                   	G1P promotes pentose phosphate pathway in CD8+ memory T cells via glycogen-G6PD
ST:STUDY_TITLE                   	phase separation and compartmentalization
ST:STUDY_SUMMARY                 	Glucose-6-phosphate (G6P) is a key metabolic molecule that regulates reactive
ST:STUDY_SUMMARY                 	oxygen species (ROS) homeostasis by initiating the pentose phosphate pathway
ST:STUDY_SUMMARY                 	(PPP) to generate NADPH that converts H2O2 to water by providing hydrogen. While
ST:STUDY_SUMMARY                 	both glucose phosphorylation and glycogenolysis result in G6P production, here
ST:STUDY_SUMMARY                 	we show that G6P derived from glycogenolysis, rather than glucose
ST:STUDY_SUMMARY                 	phosphorylation, flows to PPP for ROS clearance in CD8+ memory T (Tm) cells and
ST:STUDY_SUMMARY                 	inflammatory macrophages. Mechanistically, glycogenolysis-produced G1P
ST:STUDY_SUMMARY                 	allosterically induces G6P dehydrogenase (G6PD) binding to glycogen, which
ST:STUDY_SUMMARY                 	together undergo liquid-liquid phase separation (LLPS) and recruit PPP enzymes,
ST:STUDY_SUMMARY                 	resulting in a compartmentalized reaction cascade. Based on mechanistic
ST:STUDY_SUMMARY                 	elucidation, we demonstrated that G1P can act as an antitumor immunotherapeutic
ST:STUDY_SUMMARY                 	agent by modulating memory fitness and maintenance of tumor-reactive CD8+ T
ST:STUDY_SUMMARY                 	cells in mice. These findings revealed an unusual function of glycogen
ST:STUDY_SUMMARY                 	metabolism, which is of paramount importance in the regulation of PPP and redox
ST:STUDY_SUMMARY                 	homeostasis in cells.
ST:INSTITUTE                     	Peking Union Medical College
ST:LAST_NAME                     	Zhang
ST:FIRST_NAME                    	Chaoying
ST:ADDRESS                       	No. 5, Dongdan santiao, Dongcheng District, Beijing, Beijing, Beijing, 100005,
ST:ADDRESS                       	China
ST:EMAIL                         	zhouyaboqq@gmail.com
ST:PHONE                         	860169156464
#SUBJECT
SU:SUBJECT_TYPE                  	Mammal
SU:SUBJECT_SPECIES               	Mus musculus
SU:TAXONOMY_ID                   	10090
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data
SUBJECT_SAMPLE_FACTORS           	-	Fig_1A_HKi_1	Experiment title:Experiment 1 | Genotype:Wild Type | Treatment condition:HK inhibitor Lonidamine (50 μM) | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.1A_HKi_1.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_1A_HKi_2	Experiment title:Experiment 1 | Genotype:Wild Type | Treatment condition:HK inhibitor Lonidamine (50 μM) | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.1A_HKi_2.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_1A_HKi_3	Experiment title:Experiment 1 | Genotype:Wild Type | Treatment condition:HK inhibitor Lonidamine (50 μM) | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.1A_HKi_3.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_1A_VEC_1	Experiment title:Experiment 1 | Genotype:Wild Type | Treatment condition:control treatment | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.1A_VEC_1.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_1A_VEC_2	Experiment title:Experiment 1 | Genotype:Wild Type | Treatment condition:control treatment | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.1A_VEC_2.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_1A_VEC_3	Experiment title:Experiment 1 | Genotype:Wild Type | Treatment condition:control treatment | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.1A_VEC_3.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_1b_2DG_1	Experiment title:Experiment 1 | Genotype:Wild Type | Treatment condition:2-deoxy-D-glucose (2-DG, 1 mM) | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.1b.2DG.1.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_1b_2DG_2	Experiment title:Experiment 1 | Genotype:Wild Type | Treatment condition:2-deoxy-D-glucose (2-DG, 1 mM) | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.1b.2DG.2.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_1b_2DG_3	Experiment title:Experiment 1 | Genotype:Wild Type | Treatment condition:2-deoxy-D-glucose (2-DG, 1 mM) | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.1b.2DG.3.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_1b_vec_1	Experiment title:Experiment 1 | Genotype:Wild Type | Treatment condition:control treatment | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.1b.vec.1.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_1b_vec_2	Experiment title:Experiment 1 | Genotype:Wild Type | Treatment condition:control treatment | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.1b.vec.2.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_1b_vec_3	Experiment title:Experiment 1 | Genotype:Wild Type | Treatment condition:control treatment | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.1b.vec.3.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig1e_0h_1	Experiment title:Experiment 2 | Genotype:Wild Type | Treatment condition:13C_glucose treatment for 0h | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig1e.0h_1.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig1e_0h_2	Experiment title:Experiment 2 | Genotype:Wild Type | Treatment condition:13C_glucose treatment for 0h | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig1e.0h_2.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig1e_0h_3	Experiment title:Experiment 2 | Genotype:Wild Type | Treatment condition:13C_glucose treatment for 0h | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig1e.0h_3.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig1e_2h_1	Experiment title:Experiment 2 | Genotype:Wild Type | Treatment condition:13C_glucose treatment for 2h | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig1e.2h_1.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig1e_2h_2	Experiment title:Experiment 2 | Genotype:Wild Type | Treatment condition:13C_glucose treatment for 2h | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig1e.2h_2.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig1e_2h_3	Experiment title:Experiment 2 | Genotype:Wild Type | Treatment condition:13C_glucose treatment for 2h | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig1e.2h_3.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig1e_4h_1	Experiment title:Experiment 2 | Genotype:Wild Type | Treatment condition:13C_glucose treatment for 4h | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig1e.4h_1.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig1e_4h_2	Experiment title:Experiment 2 | Genotype:Wild Type | Treatment condition:13C_glucose treatment for 4h | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig1e.4h_2.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig1e_4h_3	Experiment title:Experiment 2 | Genotype:Wild Type | Treatment condition:13C_glucose treatment for 4h | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig1e.4h_3.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_1f_sgPYGB2H_1	Experiment title:Experiment 3 | Genotype:sgPygb | Treatment condition:13C_glucose treatment for 2h | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.1f.sgPYGB2H_1.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_1f_sgPYGB2H_2	Experiment title:Experiment 3 | Genotype:sgPygb | Treatment condition:13C_glucose treatment for 2h | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.1f.sgPYGB2H_2.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_1f_sgPYGB2H_3	Experiment title:Experiment 3 | Genotype:sgPygb | Treatment condition:13C_glucose treatment for 2h | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.1f.sgPYGB2H_3.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_1f_SGCON_2h1	Experiment title:Experiment 3 | Genotype:Wild Type | Treatment condition:13C_glucose treatment for 2h | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.1f_SGCON_2h1.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_1f_SGCON_2h2	Experiment title:Experiment 3 | Genotype:Wild Type | Treatment condition:13C_glucose treatment for 2h | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.1f_SGCON_2h2.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_1f_SGCON_2h3	Experiment title:Experiment 3 | Genotype:Wild Type | Treatment condition:13C_glucose treatment for 2h | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.1f_SGCON_2h3.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_1f_SGCON_4h1	Experiment title:Experiment 3 | Genotype:Wild Type | Treatment condition:13C_glucose treatment for 4h | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.1f_SGCON_4h1.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_1f_SGCON_4h2	Experiment title:Experiment 3 | Genotype:Wild Type | Treatment condition:13C_glucose treatment for 4h | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.1f_SGCON_4h2.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_1f_SGCON_4h3	Experiment title:Experiment 3 | Genotype:Wild Type | Treatment condition:13C_glucose treatment for 4h | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.1f_SGCON_4h3.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_1f_SGpygb_4h_1	Experiment title:Experiment 3 | Genotype:sgPygb | Treatment condition:13C_glucose treatment for 4h | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.1f_SGpygb_4h_1.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_1f_SGpygb_4h_2	Experiment title:Experiment 3 | Genotype:sgPygb | Treatment condition:13C_glucose treatment for 4h | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.1f_SGpygb_4h_2.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_1f_SGpygb_4h_3	Experiment title:Experiment 3 | Genotype:sgPygb | Treatment condition:13C_glucose treatment for 4h | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.1f_SGpygb_4h_3.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_1G_SGCTRL_1	Experiment title:Experiment 4 | Genotype:Wild Type | Treatment condition:control sgRNA | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.1G.SGCTRL_1.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_1G_SGCTRL_2	Experiment title:Experiment 4 | Genotype:Wild Type | Treatment condition:control sgRNA | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.1G.SGCTRL_2.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_1G_SGCTRL_3	Experiment title:Experiment 4 | Genotype:Wild Type | Treatment condition:control sgRNA | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.1G.SGCTRL_3.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_1G_SGpygb_1	Experiment title:Experiment 4 | Genotype:sgPygb | Treatment condition:PYGB sgRNA | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.1G.SGpygb_1.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_1G_SGpygb_2	Experiment title:Experiment 4 | Genotype:sgPygb | Treatment condition:PYGB sgRNA | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.1G.SGpygb_2.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_1G_SGpygb_3	Experiment title:Experiment 4 | Genotype:sgPygb | Treatment condition:PYGB sgRNA | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.1G.SGpygb_3.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_1G_SGpygb_G1P_1	Experiment title:Experiment 4 | Genotype:sgPygb | Treatment condition:PYGB sgRNA and G1P LNP treatment | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.1G.SGpygb_G1P_1.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_1G_SGpygb_G1P_2	Experiment title:Experiment 4 | Genotype:sgPygb | Treatment condition:PYGB sgRNA and G1P LNP treatment | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.1G.SGpygb_G1P_2.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_1G_SGpygb_G1P_3	Experiment title:Experiment 4 | Genotype:sgPygb | Treatment condition:PYGB sgRNA and G1P LNP treatment | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.1G.SGpygb_G1P_3.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_1o_Blank NP1	Experiment title:Experiment 5 | Genotype:Wild Type | Treatment condition:control NP | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.1o_Blank NP1.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_1o_Blank NP2	Experiment title:Experiment 5 | Genotype:Wild Type | Treatment condition:control NP | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.1o_Blank NP2.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_1o_Blank NP3	Experiment title:Experiment 5 | Genotype:Wild Type | Treatment condition:control NP | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.1o_Blank NP3.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_1o_G1P NP1	Experiment title:Experiment 5 | Genotype:Wild Type | Treatment condition:G1P LNP treatment | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.1o_G1P NP1.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_1o_G1P NP2	Experiment title:Experiment 5 | Genotype:Wild Type | Treatment condition:G1P LNP treatment | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.1o_G1P NP2.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_1o_G1P NP3	Experiment title:Experiment 5 | Genotype:Wild Type | Treatment condition:G1P LNP treatment | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.1o_G1P NP3.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_4i_ctrl_1	Experiment title:Experiment 6 | Genotype:Wild Type | Treatment condition:control treatment | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.4i.ctrl_1.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_4i_ctrl_2	Experiment title:Experiment 6 | Genotype:Wild Type | Treatment condition:control treatment | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.4i.ctrl_2.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_4i_ctrl_3	Experiment title:Experiment 6 | Genotype:Wild Type | Treatment condition:control treatment | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.4i.ctrl_3.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_4i_Hex_1	Experiment title:Experiment 6 | Genotype:Wild Type | Treatment condition:HEX treatment | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.4i.Hex_1.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_4i_Hex_2	Experiment title:Experiment 6 | Genotype:Wild Type | Treatment condition:HEX treatment | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.4i.Hex_2.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_4i_Hex_3	Experiment title:Experiment 6 | Genotype:Wild Type | Treatment condition:HEX treatment | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.4i.Hex_3.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig5F_glycogen	Experiment title:Experiment 7 | Genotype:Wild Type | Treatment condition:Cellular glycogen isolation | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig5F.glycogen.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig5F_sup	Experiment title:Experiment 7 | Genotype:Wild Type | Treatment condition:Cytoplasmic separation | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig5F.sup.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_5h_DMSO_1	Experiment title:Experiment 8 | Genotype:Wild Type | Treatment condition:control treatment | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.5h.DMSO_1.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_5h_DMSO_2	Experiment title:Experiment 8 | Genotype:Wild Type | Treatment condition:control treatment | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.5h.DMSO_2.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_5h_DMSO_3	Experiment title:Experiment 8 | Genotype:Wild Type | Treatment condition:control treatment | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.5h.DMSO_3.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_5h_GPI_1	Experiment title:Experiment 8 | Genotype:Wild Type | Treatment condition:GPI treatment | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.5h.GPI_1.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_5h_GPI_2	Experiment title:Experiment 8 | Genotype:Wild Type | Treatment condition:GPI treatment | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.5h.GPI_2.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_5h_GPI_3	Experiment title:Experiment 8 | Genotype:Wild Type | Treatment condition:GPI treatment | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.5h.GPI_3.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_6L_blank_1	Experiment title:Experiment 9 | Genotype:Wild Type | Treatment condition:control NP | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.6L.blank_1.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_6L_blank_2	Experiment title:Experiment 9 | Genotype:Wild Type | Treatment condition:control NP | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.6L.blank_2.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_6L_blank_3	Experiment title:Experiment 9 | Genotype:Wild Type | Treatment condition:control NP | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.6L.blank_3.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_6L_G1P_1	Experiment title:Experiment 9 | Genotype:Wild Type | Treatment condition:G1P LNP treatment | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.6L.G1P_1.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_6L_G1P_2	Experiment title:Experiment 9 | Genotype:Wild Type | Treatment condition:G1P LNP treatment | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.6L.G1P_2.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_6L_G1P_3	Experiment title:Experiment 9 | Genotype:Wild Type | Treatment condition:G1P LNP treatment | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.6L.G1P_3.raw
SUBJECT_SAMPLE_FACTORS           	-	FigS1A_1	Experiment title:Experiment 10 | Genotype:Wild Type | Treatment condition:Cellular glycogen isolation | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=FigS1A_1.raw
SUBJECT_SAMPLE_FACTORS           	-	FigS1A_2	Experiment title:Experiment 10 | Genotype:Wild Type | Treatment condition:Cellular glycogen isolation | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=FigS1A_2.raw
SUBJECT_SAMPLE_FACTORS           	-	FigS1A_3	Experiment title:Experiment 10 | Genotype:Wild Type | Treatment condition:Cellular glycogen isolation | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=FigS1A_3.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_S1c_GPI_2h1	Experiment title:Experiment 11 | Genotype:Wild Type | Treatment condition:GPI treatment and treat 13C glucose for 2h | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.S1c.GPI_2h1.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_S1c_GPI_2h2	Experiment title:Experiment 11 | Genotype:Wild Type | Treatment condition:GPI treatment and treat 13C glucose for 2h | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.S1c.GPI_2h2.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_S1c_GPI_2h3	Experiment title:Experiment 11 | Genotype:Wild Type | Treatment condition:GPI treatment and treat 13C glucose for 2h | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.S1c.GPI_2h3.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_S1c_GPI_4h1	Experiment title:Experiment 11 | Genotype:Wild Type | Treatment condition:GPI treatment and treat 13C glucose for 4h | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.S1c.GPI_4h1.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_S1c_GPI_4h2	Experiment title:Experiment 11 | Genotype:Wild Type | Treatment condition:GPI treatment and treat 13C glucose for 4h | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.S1c.GPI_4h2.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_S1c_GPI_4h3	Experiment title:Experiment 11 | Genotype:Wild Type | Treatment condition:GPI treatment and treat 13C glucose for 4h | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.S1c.GPI_4h3.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_S1c_vec_2h1	Experiment title:Experiment 11 | Genotype:Wild Type | Treatment condition:control treatment and treat 13C glucose for 2h | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.S1c.vec_2h1.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_S1c_vec_2h2	Experiment title:Experiment 11 | Genotype:Wild Type | Treatment condition:control treatment and treat 13C glucose for 2h | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.S1c.vec_2h2.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_S1c_vec_2h3	Experiment title:Experiment 11 | Genotype:Wild Type | Treatment condition:control treatment and treat 13C glucose for 2h | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.S1c.vec_2h3.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_S1c_vec_4h1	Experiment title:Experiment 11 | Genotype:Wild Type | Treatment condition:control treatment and treat 13C glucose for 4h | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.S1c.vec_4h1.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_S1c_vec_4h2	Experiment title:Experiment 11 | Genotype:Wild Type | Treatment condition:control treatment and treat 13C glucose for 4h | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.S1c.vec_4h2.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_S1c_vec_4h3	Experiment title:Experiment 11 | Genotype:Wild Type | Treatment condition:control treatment and treat 13C glucose for 4h | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.S1c.vec_4h3.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_S1E_GYSKO1_2h1	Experiment title:Experiment 12 | Genotype:sgGys1 | Treatment condition:GYS1 sgRNA and and treat 13C glucose for 2h | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.S1E.GYSKO1_2h1.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_S1E_GYSKO1_2h2	Experiment title:Experiment 12 | Genotype:sgGys1 | Treatment condition:GYS1 sgRNA and and treat 13C glucose for 2h | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.S1E.GYSKO1_2h2.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_S1E_GYSKO1_2h3	Experiment title:Experiment 12 | Genotype:sgGys1 | Treatment condition:GYS1 sgRNA and and treat 13C glucose for 2h | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.S1E.GYSKO1_2h3.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_S1E_SGCON_2h1	Experiment title:Experiment 12 | Genotype:Wild Type | Treatment condition:control sgRNA and and treat 13C glucose for 2h | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.S1E.SGCON_2h1.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_S1E_SGCON_2h2	Experiment title:Experiment 12 | Genotype:Wild Type | Treatment condition:control sgRNA and and treat 13C glucose for 2h | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.S1E.SGCON_2h2.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_S1E_SGCON_2h3	Experiment title:Experiment 12 | Genotype:Wild Type | Treatment condition:control sgRNA and and treat 13C glucose for 2h | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.S1E.SGCON_2h3.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_S1E_SGCON_4h1	Experiment title:Experiment 12 | Genotype:Wild Type | Treatment condition:control sgRNA and and treat 13C glucose for 4h | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.S1E.SGCON_4h1.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_S1E_SGCON_4h2	Experiment title:Experiment 12 | Genotype:Wild Type | Treatment condition:control sgRNA and and treat 13C glucose for 4h | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.S1E.SGCON_4h2.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_S1E_SGCON_4h3	Experiment title:Experiment 12 | Genotype:Wild Type | Treatment condition:control sgRNA and and treat 13C glucose for 4h | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.S1E.SGCON_4h3.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_S1E_SGgys1_4h1	Experiment title:Experiment 12 | Genotype:sgGys1 | Treatment condition:GYS1 sgRNA and and treat 13C glucose for 4h | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.S1E.SGgys1_4h1.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_S1E_SGgys1_4h2	Experiment title:Experiment 12 | Genotype:sgGys1 | Treatment condition:GYS1 sgRNA and and treat 13C glucose for 4h | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.S1E.SGgys1_4h2.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_S1E_SGgys1_4h3	Experiment title:Experiment 12 | Genotype:sgGys1 | Treatment condition:GYS1 sgRNA and and treat 13C glucose for 4h | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.S1E.SGgys1_4h3.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_S1G_SGCTRL_1	Experiment title:Experiment 13 | Genotype:Wild Type | Treatment condition:control sgRNA | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.S1G.SGCTRL_1.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_S1G_SGCTRL_2	Experiment title:Experiment 13 | Genotype:Wild Type | Treatment condition:control sgRNA | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.S1G.SGCTRL_2.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_S1G_SGCTRL_3	Experiment title:Experiment 13 | Genotype:Wild Type | Treatment condition:control sgRNA | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.S1G.SGCTRL_3.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_S1G_SGGYS1_1	Experiment title:Experiment 13 | Genotype:sgGys1 | Treatment condition:SG gys1 sgRNA | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.S1G.SGGYS1_1.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_S1G_SGGYS1_2	Experiment title:Experiment 13 | Genotype:sgGys1 | Treatment condition:SG gys1 sgRNA | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.S1G.SGGYS1_2.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_S1G_SGGYS1_3	Experiment title:Experiment 13 | Genotype:sgGys1 | Treatment condition:SG gys1 sgRNA | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.S1G.SGGYS1_3.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_S1H_SGCTRL_1	Experiment title:Experiment 13 | Genotype:Wild Type | Treatment condition:control sgRNA | Cell type:BMDM | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.S1H.SGCTRL_1.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_S1H_SGCTRL_2	Experiment title:Experiment 13 | Genotype:Wild Type | Treatment condition:control sgRNA | Cell type:BMDM | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.S1H.SGCTRL_2.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_S1H_SGCTRL_3	Experiment title:Experiment 13 | Genotype:Wild Type | Treatment condition:control sgRNA | Cell type:BMDM | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.S1H.SGCTRL_3.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_S1H_SGGYS1_1	Experiment title:Experiment 13 | Genotype:sgGys1 | Treatment condition:SG gys1 sgRNA | Cell type:BMDM | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.S1H.SGGYS1_1.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_S1H_SGGYS1_2	Experiment title:Experiment 13 | Genotype:sgGys1 | Treatment condition:SG gys1 sgRNA | Cell type:BMDM | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.S1H.SGGYS1_2.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_S1H_SGGYS1_3	Experiment title:Experiment 13 | Genotype:sgGys1 | Treatment condition:SG gys1 sgRNA | Cell type:BMDM | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.S1H.SGGYS1_3.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_S1i_dmso_1	Experiment title:Experiment 14 | Genotype:Wild Type | Treatment condition:control treatment | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.S1i.dmso_1.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_S1i_dmso_2	Experiment title:Experiment 14 | Genotype:Wild Type | Treatment condition:control treatment | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.S1i.dmso_2.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_S1i_dmso_3	Experiment title:Experiment 14 | Genotype:Wild Type | Treatment condition:control treatment | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.S1i.dmso_3.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_S1i_Gpi_1	Experiment title:Experiment 14 | Genotype:Wild Type | Treatment condition:GPI treatment | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.S1i.Gpi_1.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_S1i_Gpi_2	Experiment title:Experiment 14 | Genotype:Wild Type | Treatment condition:GPI treatment | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.S1i.Gpi_2.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_S1i_Gpi_3	Experiment title:Experiment 14 | Genotype:Wild Type | Treatment condition:GPI treatment | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.S1i.Gpi_3.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_S1i_Gpi_g1p_1	Experiment title:Experiment 14 | Genotype:Wild Type | Treatment condition:GPI and G1P treatment | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.S1i.Gpi_g1p_1.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_S1i_Gpi_g1p_2	Experiment title:Experiment 14 | Genotype:Wild Type | Treatment condition:GPI and G1P treatment | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.S1i.Gpi_g1p_2.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_S1i_Gpi_g1p_3	Experiment title:Experiment 14 | Genotype:Wild Type | Treatment condition:GPI and G1P treatment | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.S1i.Gpi_g1p_3.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_S1K_SGNC_1	Experiment title:Experiment 15 | Genotype:Wild Type | Treatment condition:control sgRNA | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.S1K.SGNC_1.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_S1K_SGNC_2	Experiment title:Experiment 15 | Genotype:Wild Type | Treatment condition:control sgRNA | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.S1K.SGNC_2.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_S1K_SGNC_3	Experiment title:Experiment 15 | Genotype:Wild Type | Treatment condition:control sgRNA | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.S1K.SGNC_3.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_S1K_SGPGM1_1	Experiment title:Experiment 15 | Genotype:sgPGM1 | Treatment condition:PGM1 sgRNA | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.S1K.SGPGM1_1.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_S1K_SGPGM1_2	Experiment title:Experiment 15 | Genotype:sgPGM1 | Treatment condition:PGM1 sgRNA | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.S1K.SGPGM1_2.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_S1K_SGPGM1_3	Experiment title:Experiment 15 | Genotype:sgPGM1 | Treatment condition:PGM1 sgRNA | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.S1K.SGPGM1_3.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_S1K_SGPGM1_G6P_1	Experiment title:Experiment 15 | Genotype:sgPGM1 | Treatment condition:PGM1 sgRNA and G6P LNP treatment | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.S1K.SGPGM1_G6P_1.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_S1K_SGPGM1_G6P_2	Experiment title:Experiment 15 | Genotype:sgPGM1 | Treatment condition:PGM1 sgRNA and G6P LNP treatment | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.S1K.SGPGM1_G6P_2.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_S1K_SGPGM1_G6P_3	Experiment title:Experiment 15 | Genotype:sgPGM1 | Treatment condition:PGM1 sgRNA and G6P LNP treatment | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.S1K.SGPGM1_G6P_3.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_S1L_PGM1SG_1	Experiment title:Experiment 15 | Genotype:sgPGM1 | Treatment condition:PGM1 sgRNA | Cell type:BMDM | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.S1L.PGM1SG_1.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_S1L_PGM1SG_2	Experiment title:Experiment 15 | Genotype:sgPGM1 | Treatment condition:PGM1 sgRNA | Cell type:BMDM | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.S1L.PGM1SG_2.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_S1L_PGM1SG_3	Experiment title:Experiment 15 | Genotype:sgPGM1 | Treatment condition:PGM1 sgRNA | Cell type:BMDM | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.S1L.PGM1SG_3.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_S1L_PGM1SG_G6P_1	Experiment title:Experiment 15 | Genotype:sgPGM1 | Treatment condition:PGM1 sgRNA and G6P LNP treatment | Cell type:BMDM | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.S1L.PGM1SG_G6P_1.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_S1L_PGM1SG_G6P_2	Experiment title:Experiment 15 | Genotype:sgPGM1 | Treatment condition:PGM1 sgRNA and G6P LNP treatment | Cell type:BMDM | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.S1L.PGM1SG_G6P_2.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_S1L_PGM1SG_G6P_3	Experiment title:Experiment 15 | Genotype:sgPGM1 | Treatment condition:PGM1 sgRNA and G6P LNP treatment | Cell type:BMDM | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.S1L.PGM1SG_G6P_3.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_S1L_SGNC_1	Experiment title:Experiment 15 | Genotype:Wild Type | Treatment condition:control sgRNA | Cell type:BMDM | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.S1L.SGNC_1.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_S1L_SGNC_2	Experiment title:Experiment 15 | Genotype:Wild Type | Treatment condition:control sgRNA | Cell type:BMDM | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.S1L.SGNC_2.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_S1L_SGNC_3	Experiment title:Experiment 15 | Genotype:Wild Type | Treatment condition:control sgRNA | Cell type:BMDM | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.S1L.SGNC_3.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig1_S1O_GPI_1	Experiment title:Experiment 16 | Genotype:Wild Type | Treatment condition:control treatment | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig1.S1O.GPI_1.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig1_S1O_GPI_2	Experiment title:Experiment 16 | Genotype:Wild Type | Treatment condition:control treatment | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig1.S1O.GPI_2.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig1_S1O_GPI_3	Experiment title:Experiment 16 | Genotype:Wild Type | Treatment condition:control treatment | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig1.S1O.GPI_3.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig1_S1O_VEC_1	Experiment title:Experiment 16 | Genotype:Wild Type | Treatment condition:GPI treatment | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig1.S1O.VEC_1.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig1_S1O_VEC_2	Experiment title:Experiment 16 | Genotype:Wild Type | Treatment condition:GPI treatment | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig1.S1O.VEC_2.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig1_S1O_VEC_3	Experiment title:Experiment 16 | Genotype:Wild Type | Treatment condition:GPI treatment | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig1.S1O.VEC_3.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig1_S1S_BLANK NP_1	Experiment title:Experiment 17 | Genotype:Wild Type | Treatment condition:control NP | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig1.S1S.BLANK NP_1.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig1_S1S_BLANK NP_2	Experiment title:Experiment 17 | Genotype:Wild Type | Treatment condition:control NP | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig1.S1S.BLANK NP_2.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig1_S1S_BLANK NP_3	Experiment title:Experiment 17 | Genotype:Wild Type | Treatment condition:control NP | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig1.S1S.BLANK NP_3.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig1_S1S_G1P NP_1	Experiment title:Experiment 17 | Genotype:Wild Type | Treatment condition:G1P LNP treatment | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig1.S1S.G1P NP_1.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig1_S1S_G1P NP_2	Experiment title:Experiment 17 | Genotype:Wild Type | Treatment condition:G1P LNP treatment | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig1.S1S.G1P NP_2.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig1_S1S_G1P NP_3	Experiment title:Experiment 17 | Genotype:Wild Type | Treatment condition:G1P LNP treatment | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig1.S1S.G1P NP_3.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_S4i_D421A_1	Experiment title:Experiment 18 | Genotype:D421A | Treatment condition:G6PD D421 KI | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.S4i.D421A_1.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_S4i_D421A_2	Experiment title:Experiment 18 | Genotype:D421A | Treatment condition:G6PD D421 KI | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.S4i.D421A_2.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_S4i_D421A_3	Experiment title:Experiment 18 | Genotype:D421A | Treatment condition:G6PD D421 KI | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.S4i.D421A_3.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_S4i_WT_1	Experiment title:Experiment 18 | Genotype:Wild Type | Treatment condition:G6PD WT KI | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.S4i.WT_1.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_S4i_WT_2	Experiment title:Experiment 18 | Genotype:Wild Type | Treatment condition:G6PD WT KI | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.S4i.WT_2.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_S4i_WT_3	Experiment title:Experiment 18 | Genotype:Wild Type | Treatment condition:G6PD WT KI | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.S4i.WT_3.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_S4m_DMSO_1	Experiment title:Experiment 19 | Genotype:Wild Type | Treatment condition:control treatment | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.S4m.DMSO_1.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_S4m_DMSO_2	Experiment title:Experiment 19 | Genotype:Wild Type | Treatment condition:control treatment | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.S4m.DMSO_2.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_S4m_DMSO_3	Experiment title:Experiment 19 | Genotype:Wild Type | Treatment condition:control treatment | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.S4m.DMSO_3.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_S4m_GPI_1	Experiment title:Experiment 19 | Genotype:Wild Type | Treatment condition:GPI treatment | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.S4m.GPI_1.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_S4m_GPI_2	Experiment title:Experiment 19 | Genotype:Wild Type | Treatment condition:GPI treatment | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.S4m.GPI_2.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_S4m_GPI_3	Experiment title:Experiment 19 | Genotype:Wild Type | Treatment condition:GPI treatment | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.S4m.GPI_3.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_S5K_DMSO_1	Experiment title:Experiment 20 | Genotype:Wild Type | Treatment condition:control treatment | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.S5K.DMSO_1.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_S5K_DMSO_2	Experiment title:Experiment 20 | Genotype:Wild Type | Treatment condition:control treatment | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.S5K.DMSO_2.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_S5K_DMSO_3	Experiment title:Experiment 20 | Genotype:Wild Type | Treatment condition:control treatment | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.S5K.DMSO_3.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_S5K_GPI_1	Experiment title:Experiment 20 | Genotype:Wild Type | Treatment condition:GPI treatment | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.S5K.GPI_1.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_S5K_GPI_2	Experiment title:Experiment 20 | Genotype:Wild Type | Treatment condition:GPI treatment | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.S5K.GPI_2.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_S5K_GPI_3	Experiment title:Experiment 20 | Genotype:Wild Type | Treatment condition:GPI treatment | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.S5K.GPI_3.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_S5l_ctrl_1	Experiment title:Experiment 21 | Genotype:Wild Type | Treatment condition:control treatment | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.S5l.ctrl_1.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_S5l_ctrl_2	Experiment title:Experiment 21 | Genotype:Wild Type | Treatment condition:control treatment | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.S5l.ctrl_2.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_S5l_ctrl_3	Experiment title:Experiment 21 | Genotype:Wild Type | Treatment condition:control treatment | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.S5l.ctrl_3.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_S5l_Hex_1	Experiment title:Experiment 21 | Genotype:Wild Type | Treatment condition:HEX treatment | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.S5l.Hex_1.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_S5l_Hex_2	Experiment title:Experiment 21 | Genotype:Wild Type | Treatment condition:HEX treatment | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.S5l.Hex_2.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_S5l_Hex_3	Experiment title:Experiment 21 | Genotype:Wild Type | Treatment condition:HEX treatment | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.S5l.Hex_3.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_s5m_ctrl_1	Experiment title:Experiment 21 | Genotype:Wild Type | Treatment condition:control treatment | Cell type:BMDM | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.s5m.ctrl_1.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_s5m_ctrl_2	Experiment title:Experiment 21 | Genotype:Wild Type | Treatment condition:control treatment | Cell type:BMDM | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.s5m.ctrl_2.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_s5m_ctrl_3	Experiment title:Experiment 21 | Genotype:Wild Type | Treatment condition:control treatment | Cell type:BMDM | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.s5m.ctrl_3.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_s5m_hex_1	Experiment title:Experiment 21 | Genotype:Wild Type | Treatment condition:HEX treatment | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.s5m.hex_1.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_s5m_hex_2	Experiment title:Experiment 21 | Genotype:Wild Type | Treatment condition:HEX treatment | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.s5m.hex_2.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_s5m_hex_3	Experiment title:Experiment 21 | Genotype:Wild Type | Treatment condition:HEX treatment | Cell type:CD8 T cell | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.s5m.hex_3.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_S6S_BLANK_1	Experiment title:Experiment 9 | Genotype:Wild Type | Treatment condition:control NP | Cell type:tumor-associated Macrophages | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.S6S.BLANK_1.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_S6S_BLANK_2	Experiment title:Experiment 9 | Genotype:Wild Type | Treatment condition:control NP | Cell type:tumor-associated Macrophages | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.S6S.BLANK_2.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_S6S_BLANK_3	Experiment title:Experiment 9 | Genotype:Wild Type | Treatment condition:control NP | Cell type:tumor-associated Macrophages | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.S6S.BLANK_3.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_S6S_G1P_1	Experiment title:Experiment 9 | Genotype:Wild Type | Treatment condition:G1P LNP treatment | Cell type:tumor-associated Macrophages | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.S6S.G1P_1.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_S6S_G1P_2	Experiment title:Experiment 9 | Genotype:Wild Type | Treatment condition:G1P LNP treatment | Cell type:tumor-associated Macrophages | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.S6S.G1P_2.raw
SUBJECT_SAMPLE_FACTORS           	-	Fig_S6S_G1P_3	Experiment title:Experiment 9 | Genotype:Wild Type | Treatment condition:G1P LNP treatment | Cell type:tumor-associated Macrophages | Sample source:Immune cell	RAW_FILE_NAME(Raw file name)=Fig.S6S.G1P_3.raw
#COLLECTION
CO:COLLECTION_SUMMARY            	Murine CD8⁺ T cells were isolated from the spleens of C57BL/6J mice using
CO:COLLECTION_SUMMARY            	negative selection with magnetic-activated cell sorting (MACS; Mouse CD8⁺ T
CO:COLLECTION_SUMMARY            	Cell Isolation Kit, Miltenyi Biotec, Germany). For the generation of stable gene
CO:COLLECTION_SUMMARY            	knockouts in CD8⁺ T cells or interferon-gamma (IFN-γ)-stimulated immortalized
CO:COLLECTION_SUMMARY            	bone marrow-derived macrophages (iBMDMs), single-guide RNAs (sgRNAs) were
CO:COLLECTION_SUMMARY            	synthesized using the HiScribe® Quick T7 High Yield RNA Synthesis Kit (New
CO:COLLECTION_SUMMARY            	England Biolabs, #E2050, USA). A total of 1 × 10⁶ T cells or
CO:COLLECTION_SUMMARY            	IFN-γ-stimulated iBMDMs were resuspended in 20 µL of P3 Primary Cell
CO:COLLECTION_SUMMARY            	Nucleofector Solution (Lonza, for the 4D-Nucleofector system), and
CO:COLLECTION_SUMMARY            	co-electroporated with 0.5 µg of sgRNA and recombinant Cas9 protein. Isolated
CO:COLLECTION_SUMMARY            	CD8⁺ T cells were activated using anti-CD3/CD28 magnetic beads (Thermo Fisher
CO:COLLECTION_SUMMARY            	Scientific, #11453) according to the manufacturer’s instructions. Briefly, 1
CO:COLLECTION_SUMMARY            	× 10⁶ purified splenic OT-I CD8⁺ T cells were cultured in 1 mL RPMI 1640
CO:COLLECTION_SUMMARY            	medium containing 25 µL of pre-washed anti-CD3/CD28 beads and 10 ng/mL
CO:COLLECTION_SUMMARY            	interleukin-2 (IL-2; PeproTech, #212-12) for three days. Following activation,
CO:COLLECTION_SUMMARY            	cells were harvested, washed three times with RPMI 1640 medium, and further
CO:COLLECTION_SUMMARY            	cultured at 2 × 10⁵ cells/mL in the presence of 10 ng/mL interleukin-15
CO:COLLECTION_SUMMARY            	(IL-15; PeproTech, #210-15) in 12-well culture plates (Corning, #3336) for an
CO:COLLECTION_SUMMARY            	additional four days to induce memory CD8⁺ T cell (T_m) differentiation. Fresh
CO:COLLECTION_SUMMARY            	medium supplemented with cytokines was replaced daily during the induction
CO:COLLECTION_SUMMARY            	process. Mouse iBMDMs were cultured in Dulbecco’s Modified Eagle Medium (DMEM;
CO:COLLECTION_SUMMARY            	Gibco, USA) supplemented with 10% fetal bovine serum (FBS). For gene knockout
CO:COLLECTION_SUMMARY            	experiments, 1 × 10⁶ IFN-γ-stimulated iBMDMs were subjected to nucleofection
CO:COLLECTION_SUMMARY            	as described above, using sgRNAs and Cas9 protein.
CO:SAMPLE_TYPE                   	Immune cell
CO:STORAGE_CONDITIONS            	On ice
#TREATMENT
TR:TREATMENT_SUMMARY             	Experiment 1: CD8⁺ memory T (T_m) cells were treated with hexokinase (HK)
TR:TREATMENT_SUMMARY             	inhibitor lonidamine (50 μM) or 2-deoxy-D-glucose (2-DG, 1 mM) for 24 hours.
TR:TREATMENT_SUMMARY             	Ribose-5-phosphate (R5P) and sedoheptulose-7-phosphate (S7P) were detected.
TR:TREATMENT_SUMMARY             	Experiment 2: CD8⁺ T_m cells were cultured in uniformly labeled 13C₆-glucose
TR:TREATMENT_SUMMARY             	for 7 days and then switched to unlabeled 12C-glucose for 2 or 4 hours. m+5
TR:TREATMENT_SUMMARY             	labeled R5P was detected. Experiment 3: Control (sgCtrl) or glycogen
TR:TREATMENT_SUMMARY             	phosphorylase brain isoform (sgPygb) CD8⁺ T_m cells cultured in 13C₆-glucose
TR:TREATMENT_SUMMARY             	were switched to 12C-glucose for 2 or 4 hours. m+5 labeled R5P was detected.
TR:TREATMENT_SUMMARY             	Experiment 4: sgCtrl or sgPygb CD8⁺ T_m cells were treated with
TR:TREATMENT_SUMMARY             	glucose-1-phosphate-loaded nanoparticles (G1P-NPs, 5 μM) for 24 hours. R5P and
TR:TREATMENT_SUMMARY             	S7P levels were detected. Experiment 5: CD8⁺ T_m cells pretreated with G1P-NPs
TR:TREATMENT_SUMMARY             	(5 μM) for 24 hours were cultured in 13C₆-glucose for 4 hours. m+5 R5P was
TR:TREATMENT_SUMMARY             	detected. Experiment 6: CD8⁺ T_m cells were treated with or without 5%
TR:TREATMENT_SUMMARY             	1,6-hexanediol (1,6-HD) for 10 minutes and then cultured in 13C₆-glucose for 4
TR:TREATMENT_SUMMARY             	hours. m+5 R5P was detected. Experiment 7: Glycogen was isolated and dissolved
TR:TREATMENT_SUMMARY             	from CD8⁺ T_m cells. R5P from cytosol and glycogen fractions was measured.
TR:TREATMENT_SUMMARY             	Experiment 8: CD8⁺ T_m cells pretreated with glucose-6-phosphate isomerase
TR:TREATMENT_SUMMARY             	inhibitor (GPI, 50 μM) for 12 hours were electroporated with
TR:TREATMENT_SUMMARY             	13C₆-glucose-6-phosphate (13C₆-G6P) and cultured for 4 hours. m+5 R5P was
TR:TREATMENT_SUMMARY             	analyzed. Experiment 9: C57BL/6 mice were subcutaneously inoculated with
TR:TREATMENT_SUMMARY             	1×10⁶ Lewis lung carcinoma expressing ovalbumin (LLC-OVA) cells. Eight days
TR:TREATMENT_SUMMARY             	later, mice were transferred with or without OT-I T cells and injected
TR:TREATMENT_SUMMARY             	intraperitoneally with or without CD8α-targeted G1P-NPs (50 μg/kg) every two
TR:TREATMENT_SUMMARY             	days. Mice were fasted overnight and then infused with 13C₆-glucose (12.5
TR:TREATMENT_SUMMARY             	mg/kg/min) via the tail vein for 4 hours. m+5 R5P was detected in ovalbumin
TR:TREATMENT_SUMMARY             	(OVA)-specific CD8⁺ T_m cells from lymph nodes (LNs) and tumor-infiltrating
TR:TREATMENT_SUMMARY             	macrophages. Experiment 10: CD8⁺ T_m cells were induced by interleukin-15
TR:TREATMENT_SUMMARY             	(IL-15) and cultured in 13C₆-glucose for 7 days, followed by hydrochloric acid
TR:TREATMENT_SUMMARY             	treatment. The released m+6 labeled glucose was measured. Experiment 11: CD8⁺
TR:TREATMENT_SUMMARY             	T_m cells cultured in 13C₆-glucose were pretreated with GPI (50 μM) for 12
TR:TREATMENT_SUMMARY             	hours and then switched to 12C-glucose for 2 or 4 hours. m+5 labeled R5P was
TR:TREATMENT_SUMMARY             	detected. Experiment 12: sgCtrl or glycogen synthase 1 (sgGys1) CD8⁺ T_m cells
TR:TREATMENT_SUMMARY             	cultured in 13C₆-glucose were switched to 12C-glucose for 2 or 4 hours. m+5
TR:TREATMENT_SUMMARY             	labeled R5P was detected. Experiment 13: sgCtrl or sgGys1 CD8⁺ T_m cells or
TR:TREATMENT_SUMMARY             	immortalized bone marrow-derived macrophages (iBMDM) were cultured in
TR:TREATMENT_SUMMARY             	13C₆-glucose for 4 hours. m+5 labeled R5P was detected. Experiment 14: CD8⁺
TR:TREATMENT_SUMMARY             	T_m cells pretreated with GPI (50 μM) for 12 hours were treated with G1P-NPs (5
TR:TREATMENT_SUMMARY             	μM) for 24 hours. R5P and S7P levels were measured. Experiment 15: sgCtrl or
TR:TREATMENT_SUMMARY             	phosphoglucomutase 1 (sgPgm1) CD8⁺ T_m cells or iBMDMs were treated with
TR:TREATMENT_SUMMARY             	glucose-6-phosphate-loaded nanoparticles (G6P-NPs, 5 μM) for 24 hours. R5P and
TR:TREATMENT_SUMMARY             	S7P levels were measured. Experiment 16: CD8⁺ T_m cells cultured in
TR:TREATMENT_SUMMARY             	13C₆-glucose for 7 days were electroporated with a
TR:TREATMENT_SUMMARY             	pcDNA-mouse-glucose-6-phosphate dehydrogenase-Flag (pcDNA-m-G6PD-Flag) plasmid.
TR:TREATMENT_SUMMARY             	Cells were then treated with or without GPI (50 μM) for 12 hours and switched
TR:TREATMENT_SUMMARY             	to 12C-glucose medium for 2 hours. After washing with phosphate-buffered saline
TR:TREATMENT_SUMMARY             	(PBS), cells were crosslinked by ultraviolet C (UVC, 254 nm) at 0.3 J/cm² and
TR:TREATMENT_SUMMARY             	lysed. Immunoprecipitation was performed using anti-Flag magnetic beads. m+6 and
TR:TREATMENT_SUMMARY             	m+0 G1P bound to beads were detected. Experiment 17: iBMDMs pretreated with
TR:TREATMENT_SUMMARY             	G1P-NPs (5 μM) for 24 hours were cultured in 13C₆-glucose for 4 hours. m+5
TR:TREATMENT_SUMMARY             	labeled R5P was detected. Experiment 18: Wild-type G6PD (G6PDWT) or knock-in
TR:TREATMENT_SUMMARY             	G6PDD421A CD8⁺ T_m cells were cultured in 13C₆-glucose for 4 hours. m+5
TR:TREATMENT_SUMMARY             	labeled R5P was detected. Experiment 19: CD8⁺ T_m cells pretreated with GPI
TR:TREATMENT_SUMMARY             	(50 μM) for 12 hours were cultured in 13C₆-glucose for 2 hours. m+6 labeled
TR:TREATMENT_SUMMARY             	uridine diphosphate glucose (UDPG) was detected. Experiment 20: iBMDMs
TR:TREATMENT_SUMMARY             	pretreated with GPI (50 μM) for 12 hours were electroporated with 13C₆-G6P
TR:TREATMENT_SUMMARY             	for 4 hours. m+5 R5P was analyzed. Experiment 21: CD8⁺ T_m cells or iBMDMs
TR:TREATMENT_SUMMARY             	pretreated with 5% 1,6-HD for 10 minutes were electroporated with 13C₆-G6P for
TR:TREATMENT_SUMMARY             	4 hours. m+5 R5P was analyzed.
TR:TREATMENT_PROTOCOL_FILENAME   	BoHuang_Lab_C13_Extraction_and_Analysis_Protocol.pdf
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	For metabolite analysis, cells were washed twice with ice-cold
SP:SAMPLEPREP_SUMMARY            	phosphate-buffered saline (PBS) and extracted with ice-cold 80% methanol. The
SP:SAMPLEPREP_SUMMARY            	cell suspension was subjected to three freeze-thaw cycles using liquid nitrogen
SP:SAMPLEPREP_SUMMARY            	to ensure complete lysis. After centrifugation at 12,000 × g for 10 minutes at
SP:SAMPLEPREP_SUMMARY            	4 °C, the supernatant containing metabolites was transferred to a new tube
SP:SAMPLEPREP_SUMMARY            	and stored at −80 °C until further analysis.
SP:SAMPLEPREP_PROTOCOL_FILENAME  	BoHuang_Lab_C13_Extraction_and_Analysis_Protocol.pdf
SP:PROCESSING_STORAGE_CONDITIONS 	On ice
SP:EXTRACTION_METHOD             	Cells were washed twice with ice-cold PBS and metabolites were extracted with
SP:EXTRACTION_METHOD             	ice-cold 80% methanol.
SP:EXTRACT_STORAGE               	-80℃
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_SUMMARY        	Solvent A: 20 mM ammonium acetate and 15 mM ammonium hydroxide in water with 3%
CH:CHROMATOGRAPHY_SUMMARY        	acetonitrile, pH 9.0; Solvent B: acetonitrile
CH:CHROMATOGRAPHY_TYPE           	HILIC
CH:INSTRUMENT_NAME               	Thermo Vanquish
CH:COLUMN_NAME                   	Waters Xbridge Amide (100 × 2.1mm, 3.5um)
CH:SOLVENT_A                     	100% water/3% acetonitrile; 20 mM ammonium acetate; 15 mM ammonium hydroxide
CH:SOLVENT_B                     	100% acetonitrile
CH:FLOW_GRADIENT                 	0 min, 85% B; 1.5 min, 85% B, 5.5 min, 30% B; 8 min, 30% B, 10 min, 85% B, and
CH:FLOW_GRADIENT                 	12 min, 85% B
CH:FLOW_RATE                     	0.2 mL/min
CH:COLUMN_TEMPERATURE            	35
CH:METHODS_FILENAME              	BoHuang_Lab_C13_Extraction_and_Analysis_Protocol.pdf
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
#MS
MS:INSTRUMENT_NAME               	Thermo Q Exactive Orbitrap
MS:INSTRUMENT_TYPE               	Orbitrap
MS:MS_TYPE                       	ESI
MS:ION_MODE                      	NEGATIVE
MS:MS_COMMENTS                   	The Q Exactive mass spectrometer was operated in full MS scan mode using
MS:MS_COMMENTS                   	negative ionization (acquisition range: m/z 50–650) with a resolution of
MS:MS_COMMENTS                   	35,000. Three independent biological replicates were analyzed for each
MS:MS_COMMENTS                   	condition, and sample injection order was randomized to minimize analytical bias
MS:MS_COMMENTS                   	due to instrument drift. Data were quantified by integrating the area under the
MS:MS_COMMENTS                   	curve of each compound using the Xcalibur Qual browser. Each metabolite’s
MS:MS_COMMENTS                   	accurate mass ion and subsequent isotopic ions were extracted (EIC) using a 20
MS:MS_COMMENTS                   	ppm window.
#MS_METABOLITE_DATA
MS_METABOLITE_DATA:UNITS	Peak area
MS_METABOLITE_DATA_START
Samples	Fig_1A_HKi_1	Fig_1A_HKi_2	Fig_1A_HKi_3	Fig_1A_VEC_1	Fig_1A_VEC_2	Fig_1A_VEC_3	Fig_1b_2DG_1	Fig_1b_2DG_2	Fig_1b_2DG_3	Fig_1b_vec_1	Fig_1b_vec_2	Fig_1b_vec_3	Fig1e_0h_1	Fig1e_0h_2	Fig1e_0h_3	Fig1e_2h_1	Fig1e_2h_2	Fig1e_2h_3	Fig1e_4h_1	Fig1e_4h_2	Fig1e_4h_3	Fig_1f_sgPYGB2H_1	Fig_1f_sgPYGB2H_2	Fig_1f_sgPYGB2H_3	Fig_1f_SGCON_2h1	Fig_1f_SGCON_2h2	Fig_1f_SGCON_2h3	Fig_1f_SGCON_4h1	Fig_1f_SGCON_4h2	Fig_1f_SGCON_4h3	Fig_1f_SGpygb_4h_1	Fig_1f_SGpygb_4h_2	Fig_1f_SGpygb_4h_3	Fig_1G_SGCTRL_1	Fig_1G_SGCTRL_2	Fig_1G_SGCTRL_3	Fig_1G_SGpygb_1	Fig_1G_SGpygb_2	Fig_1G_SGpygb_3	Fig_1G_SGpygb_G1P_1	Fig_1G_SGpygb_G1P_2	Fig_1G_SGpygb_G1P_3	Fig_1o_Blank NP1	Fig_1o_Blank NP2	Fig_1o_Blank NP3	Fig_1o_G1P NP1	Fig_1o_G1P NP2	Fig_1o_G1P NP3	Fig_4i_ctrl_1	Fig_4i_ctrl_2	Fig_4i_ctrl_3	Fig_4i_Hex_1	Fig_4i_Hex_2	Fig_4i_Hex_3	Fig5F_glycogen	Fig5F_sup	Fig_5h_DMSO_1	Fig_5h_DMSO_2	Fig_5h_DMSO_3	Fig_5h_GPI_1	Fig_5h_GPI_2	Fig_5h_GPI_3	Fig_6L_blank_1	Fig_6L_blank_2	Fig_6L_blank_3	Fig_6L_G1P_1	Fig_6L_G1P_2	Fig_6L_G1P_3	FigS1A_1	FigS1A_2	FigS1A_3	Fig_S1c_GPI_2h1	Fig_S1c_GPI_2h2	Fig_S1c_GPI_2h3	Fig_S1c_GPI_4h1	Fig_S1c_GPI_4h2	Fig_S1c_GPI_4h3	Fig_S1c_vec_2h1	Fig_S1c_vec_2h2	Fig_S1c_vec_2h3	Fig_S1c_vec_4h1	Fig_S1c_vec_4h2	Fig_S1c_vec_4h3	Fig_S1E_GYSKO1_2h1	Fig_S1E_GYSKO1_2h2	Fig_S1E_GYSKO1_2h3	Fig_S1E_SGCON_2h1	Fig_S1E_SGCON_2h2	Fig_S1E_SGCON_2h3	Fig_S1E_SGCON_4h1	Fig_S1E_SGCON_4h2	Fig_S1E_SGCON_4h3	Fig_S1E_SGgys1_4h1	Fig_S1E_SGgys1_4h2	Fig_S1E_SGgys1_4h3	Fig_S1G_SGCTRL_1	Fig_S1G_SGCTRL_2	Fig_S1G_SGCTRL_3	Fig_S1G_SGGYS1_1	Fig_S1G_SGGYS1_2	Fig_S1G_SGGYS1_3	Fig_S1H_SGCTRL_1	Fig_S1H_SGCTRL_2	Fig_S1H_SGCTRL_3	Fig_S1H_SGGYS1_1	Fig_S1H_SGGYS1_2	Fig_S1H_SGGYS1_3	Fig_S1i_dmso_1	Fig_S1i_dmso_2	Fig_S1i_dmso_3	Fig_S1i_Gpi_1	Fig_S1i_Gpi_2	Fig_S1i_Gpi_3	Fig_S1i_Gpi_g1p_1	Fig_S1i_Gpi_g1p_2	Fig_S1i_Gpi_g1p_3	Fig_S1K_SGNC_1	Fig_S1K_SGNC_2	Fig_S1K_SGNC_3	Fig_S1K_SGPGM1_1	Fig_S1K_SGPGM1_2	Fig_S1K_SGPGM1_3	Fig_S1K_SGPGM1_G6P_1	Fig_S1K_SGPGM1_G6P_2	Fig_S1K_SGPGM1_G6P_3	Fig_S1L_PGM1SG_1	Fig_S1L_PGM1SG_2	Fig_S1L_PGM1SG_3	Fig_S1L_PGM1SG_G6P_1	Fig_S1L_PGM1SG_G6P_2	Fig_S1L_PGM1SG_G6P_3	Fig_S1L_SGNC_1	Fig_S1L_SGNC_2	Fig_S1L_SGNC_3	Fig1_S1O_GPI_1	Fig1_S1O_GPI_2	Fig1_S1O_GPI_3	Fig1_S1O_VEC_1	Fig1_S1O_VEC_2	Fig1_S1O_VEC_3	Fig1_S1S_BLANK NP_1	Fig1_S1S_BLANK NP_2	Fig1_S1S_BLANK NP_3	Fig1_S1S_G1P NP_1	Fig1_S1S_G1P NP_2	Fig1_S1S_G1P NP_3	Fig_S4i_D421A_1	Fig_S4i_D421A_2	Fig_S4i_D421A_3	Fig_S4i_WT_1	Fig_S4i_WT_2	Fig_S4i_WT_3	Fig_S4m_DMSO_1	Fig_S4m_DMSO_2	Fig_S4m_DMSO_3	Fig_S4m_GPI_1	Fig_S4m_GPI_2	Fig_S4m_GPI_3	Fig_S5K_DMSO_1	Fig_S5K_DMSO_2	Fig_S5K_DMSO_3	Fig_S5K_GPI_1	Fig_S5K_GPI_2	Fig_S5K_GPI_3	Fig_S5l_ctrl_1	Fig_S5l_ctrl_2	Fig_S5l_ctrl_3	Fig_S5l_Hex_1	Fig_S5l_Hex_2	Fig_S5l_Hex_3	Fig_s5m_ctrl_1	Fig_s5m_ctrl_2	Fig_s5m_ctrl_3	Fig_s5m_hex_1	Fig_s5m_hex_2	Fig_s5m_hex_3	Fig_S6S_BLANK_1	Fig_S6S_BLANK_2	Fig_S6S_BLANK_3	Fig_S6S_G1P_1	Fig_S6S_G1P_2	Fig_S6S_G1P_3	
Factors	Experiment title:Experiment 1 | Genotype:Wild Type | Treatment condition:HK inhibitor Lonidamine (50 μM) | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 1 | Genotype:Wild Type | Treatment condition:HK inhibitor Lonidamine (50 μM) | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 1 | Genotype:Wild Type | Treatment condition:HK inhibitor Lonidamine (50 μM) | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 1 | Genotype:Wild Type | Treatment condition:control treatment | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 1 | Genotype:Wild Type | Treatment condition:control treatment | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 1 | Genotype:Wild Type | Treatment condition:control treatment | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 1 | Genotype:Wild Type | Treatment condition:2-deoxy-D-glucose (2-DG, 1 mM) | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 1 | Genotype:Wild Type | Treatment condition:2-deoxy-D-glucose (2-DG, 1 mM) | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 1 | Genotype:Wild Type | Treatment condition:2-deoxy-D-glucose (2-DG, 1 mM) | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 1 | Genotype:Wild Type | Treatment condition:control treatment | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 1 | Genotype:Wild Type | Treatment condition:control treatment | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 1 | Genotype:Wild Type | Treatment condition:control treatment | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 2 | Genotype:Wild Type | Treatment condition:13C_glucose treatment for 0h | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 2 | Genotype:Wild Type | Treatment condition:13C_glucose treatment for 0h | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 2 | Genotype:Wild Type | Treatment condition:13C_glucose treatment for 0h | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 2 | Genotype:Wild Type | Treatment condition:13C_glucose treatment for 2h | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 2 | Genotype:Wild Type | Treatment condition:13C_glucose treatment for 2h | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 2 | Genotype:Wild Type | Treatment condition:13C_glucose treatment for 2h | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 2 | Genotype:Wild Type | Treatment condition:13C_glucose treatment for 4h | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 2 | Genotype:Wild Type | Treatment condition:13C_glucose treatment for 4h | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 2 | Genotype:Wild Type | Treatment condition:13C_glucose treatment for 4h | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 3 | Genotype:sgPygb | Treatment condition:13C_glucose treatment for 2h | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 3 | Genotype:sgPygb | Treatment condition:13C_glucose treatment for 2h | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 3 | Genotype:sgPygb | Treatment condition:13C_glucose treatment for 2h | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 3 | Genotype:Wild Type | Treatment condition:13C_glucose treatment for 2h | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 3 | Genotype:Wild Type | Treatment condition:13C_glucose treatment for 2h | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 3 | Genotype:Wild Type | Treatment condition:13C_glucose treatment for 2h | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 3 | Genotype:Wild Type | Treatment condition:13C_glucose treatment for 4h | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 3 | Genotype:Wild Type | Treatment condition:13C_glucose treatment for 4h | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 3 | Genotype:Wild Type | Treatment condition:13C_glucose treatment for 4h | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 3 | Genotype:sgPygb | Treatment condition:13C_glucose treatment for 4h | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 3 | Genotype:sgPygb | Treatment condition:13C_glucose treatment for 4h | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 3 | Genotype:sgPygb | Treatment condition:13C_glucose treatment for 4h | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 4 | Genotype:Wild Type | Treatment condition:control sgRNA | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 4 | Genotype:Wild Type | Treatment condition:control sgRNA | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 4 | Genotype:Wild Type | Treatment condition:control sgRNA | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 4 | Genotype:sgPygb | Treatment condition:PYGB sgRNA | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 4 | Genotype:sgPygb | Treatment condition:PYGB sgRNA | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 4 | Genotype:sgPygb | Treatment condition:PYGB sgRNA | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 4 | Genotype:sgPygb | Treatment condition:PYGB sgRNA and G1P LNP treatment | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 4 | Genotype:sgPygb | Treatment condition:PYGB sgRNA and G1P LNP treatment | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 4 | Genotype:sgPygb | Treatment condition:PYGB sgRNA and G1P LNP treatment | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 5 | Genotype:Wild Type | Treatment condition:control NP | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 5 | Genotype:Wild Type | Treatment condition:control NP | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 5 | Genotype:Wild Type | Treatment condition:control NP | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 5 | Genotype:Wild Type | Treatment condition:G1P LNP treatment | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 5 | Genotype:Wild Type | Treatment condition:G1P LNP treatment | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 5 | Genotype:Wild Type | Treatment condition:G1P LNP treatment | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 6 | Genotype:Wild Type | Treatment condition:control treatment | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 6 | Genotype:Wild Type | Treatment condition:control treatment | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 6 | Genotype:Wild Type | Treatment condition:control treatment | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 6 | Genotype:Wild Type | Treatment condition:HEX treatment | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 6 | Genotype:Wild Type | Treatment condition:HEX treatment | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 6 | Genotype:Wild Type | Treatment condition:HEX treatment | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 7 | Genotype:Wild Type | Treatment condition:Cellular glycogen isolation | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 7 | Genotype:Wild Type | Treatment condition:Cytoplasmic separation | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 8 | Genotype:Wild Type | Treatment condition:control treatment | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 8 | Genotype:Wild Type | Treatment condition:control treatment | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 8 | Genotype:Wild Type | Treatment condition:control treatment | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 8 | Genotype:Wild Type | Treatment condition:GPI treatment | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 8 | Genotype:Wild Type | Treatment condition:GPI treatment | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 8 | Genotype:Wild Type | Treatment condition:GPI treatment | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 9 | Genotype:Wild Type | Treatment condition:control NP | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 9 | Genotype:Wild Type | Treatment condition:control NP | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 9 | Genotype:Wild Type | Treatment condition:control NP | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 9 | Genotype:Wild Type | Treatment condition:G1P LNP treatment | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 9 | Genotype:Wild Type | Treatment condition:G1P LNP treatment | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 9 | Genotype:Wild Type | Treatment condition:G1P LNP treatment | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 10 | Genotype:Wild Type | Treatment condition:Cellular glycogen isolation | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 10 | Genotype:Wild Type | Treatment condition:Cellular glycogen isolation | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 10 | Genotype:Wild Type | Treatment condition:Cellular glycogen isolation | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 11 | Genotype:Wild Type | Treatment condition:GPI treatment and treat 13C glucose for 2h | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 11 | Genotype:Wild Type | Treatment condition:GPI treatment and treat 13C glucose for 2h | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 11 | Genotype:Wild Type | Treatment condition:GPI treatment and treat 13C glucose for 2h | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 11 | Genotype:Wild Type | Treatment condition:GPI treatment and treat 13C glucose for 4h | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 11 | Genotype:Wild Type | Treatment condition:GPI treatment and treat 13C glucose for 4h | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 11 | Genotype:Wild Type | Treatment condition:GPI treatment and treat 13C glucose for 4h | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 11 | Genotype:Wild Type | Treatment condition:control treatment and treat 13C glucose for 2h | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 11 | Genotype:Wild Type | Treatment condition:control treatment and treat 13C glucose for 2h | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 11 | Genotype:Wild Type | Treatment condition:control treatment and treat 13C glucose for 2h | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 11 | Genotype:Wild Type | Treatment condition:control treatment and treat 13C glucose for 4h | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 11 | Genotype:Wild Type | Treatment condition:control treatment and treat 13C glucose for 4h | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 11 | Genotype:Wild Type | Treatment condition:control treatment and treat 13C glucose for 4h | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 12 | Genotype:sgGys1 | Treatment condition:GYS1 sgRNA and and treat 13C glucose for 2h | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 12 | Genotype:sgGys1 | Treatment condition:GYS1 sgRNA and and treat 13C glucose for 2h | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 12 | Genotype:sgGys1 | Treatment condition:GYS1 sgRNA and and treat 13C glucose for 2h | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 12 | Genotype:Wild Type | Treatment condition:control sgRNA and and treat 13C glucose for 2h | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 12 | Genotype:Wild Type | Treatment condition:control sgRNA and and treat 13C glucose for 2h | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 12 | Genotype:Wild Type | Treatment condition:control sgRNA and and treat 13C glucose for 2h | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 12 | Genotype:Wild Type | Treatment condition:control sgRNA and and treat 13C glucose for 4h | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 12 | Genotype:Wild Type | Treatment condition:control sgRNA and and treat 13C glucose for 4h | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 12 | Genotype:Wild Type | Treatment condition:control sgRNA and and treat 13C glucose for 4h | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 12 | Genotype:sgGys1 | Treatment condition:GYS1 sgRNA and and treat 13C glucose for 4h | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 12 | Genotype:sgGys1 | Treatment condition:GYS1 sgRNA and and treat 13C glucose for 4h | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 12 | Genotype:sgGys1 | Treatment condition:GYS1 sgRNA and and treat 13C glucose for 4h | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 13 | Genotype:Wild Type | Treatment condition:control sgRNA | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 13 | Genotype:Wild Type | Treatment condition:control sgRNA | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 13 | Genotype:Wild Type | Treatment condition:control sgRNA | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 13 | Genotype:sgGys1 | Treatment condition:SG gys1 sgRNA | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 13 | Genotype:sgGys1 | Treatment condition:SG gys1 sgRNA | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 13 | Genotype:sgGys1 | Treatment condition:SG gys1 sgRNA | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 13 | Genotype:Wild Type | Treatment condition:control sgRNA | Cell type:BMDM | Sample source:Immune cell	Experiment title:Experiment 13 | Genotype:Wild Type | Treatment condition:control sgRNA | Cell type:BMDM | Sample source:Immune cell	Experiment title:Experiment 13 | Genotype:Wild Type | Treatment condition:control sgRNA | Cell type:BMDM | Sample source:Immune cell	Experiment title:Experiment 13 | Genotype:sgGys1 | Treatment condition:SG gys1 sgRNA | Cell type:BMDM | Sample source:Immune cell	Experiment title:Experiment 13 | Genotype:sgGys1 | Treatment condition:SG gys1 sgRNA | Cell type:BMDM | Sample source:Immune cell	Experiment title:Experiment 13 | Genotype:sgGys1 | Treatment condition:SG gys1 sgRNA | Cell type:BMDM | Sample source:Immune cell	Experiment title:Experiment 14 | Genotype:Wild Type | Treatment condition:control treatment | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 14 | Genotype:Wild Type | Treatment condition:control treatment | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 14 | Genotype:Wild Type | Treatment condition:control treatment | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 14 | Genotype:Wild Type | Treatment condition:GPI treatment | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 14 | Genotype:Wild Type | Treatment condition:GPI treatment | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 14 | Genotype:Wild Type | Treatment condition:GPI treatment | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 14 | Genotype:Wild Type | Treatment condition:GPI and G1P treatment | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 14 | Genotype:Wild Type | Treatment condition:GPI and G1P treatment | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 14 | Genotype:Wild Type | Treatment condition:GPI and G1P treatment | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 15 | Genotype:Wild Type | Treatment condition:control sgRNA | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 15 | Genotype:Wild Type | Treatment condition:control sgRNA | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 15 | Genotype:Wild Type | Treatment condition:control sgRNA | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 15 | Genotype:sgPGM1 | Treatment condition:PGM1 sgRNA | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 15 | Genotype:sgPGM1 | Treatment condition:PGM1 sgRNA | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 15 | Genotype:sgPGM1 | Treatment condition:PGM1 sgRNA | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 15 | Genotype:sgPGM1 | Treatment condition:PGM1 sgRNA and G6P LNP treatment | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 15 | Genotype:sgPGM1 | Treatment condition:PGM1 sgRNA and G6P LNP treatment | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 15 | Genotype:sgPGM1 | Treatment condition:PGM1 sgRNA and G6P LNP treatment | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 15 | Genotype:sgPGM1 | Treatment condition:PGM1 sgRNA | Cell type:BMDM | Sample source:Immune cell	Experiment title:Experiment 15 | Genotype:sgPGM1 | Treatment condition:PGM1 sgRNA | Cell type:BMDM | Sample source:Immune cell	Experiment title:Experiment 15 | Genotype:sgPGM1 | Treatment condition:PGM1 sgRNA | Cell type:BMDM | Sample source:Immune cell	Experiment title:Experiment 15 | Genotype:sgPGM1 | Treatment condition:PGM1 sgRNA and G6P LNP treatment | Cell type:BMDM | Sample source:Immune cell	Experiment title:Experiment 15 | Genotype:sgPGM1 | Treatment condition:PGM1 sgRNA and G6P LNP treatment | Cell type:BMDM | Sample source:Immune cell	Experiment title:Experiment 15 | Genotype:sgPGM1 | Treatment condition:PGM1 sgRNA and G6P LNP treatment | Cell type:BMDM | Sample source:Immune cell	Experiment title:Experiment 15 | Genotype:Wild Type | Treatment condition:control sgRNA | Cell type:BMDM | Sample source:Immune cell	Experiment title:Experiment 15 | Genotype:Wild Type | Treatment condition:control sgRNA | Cell type:BMDM | Sample source:Immune cell	Experiment title:Experiment 15 | Genotype:Wild Type | Treatment condition:control sgRNA | Cell type:BMDM | Sample source:Immune cell	Experiment title:Experiment 16 | Genotype:Wild Type | Treatment condition:control treatment | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 16 | Genotype:Wild Type | Treatment condition:control treatment | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 16 | Genotype:Wild Type | Treatment condition:control treatment | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 16 | Genotype:Wild Type | Treatment condition:GPI treatment | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 16 | Genotype:Wild Type | Treatment condition:GPI treatment | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 16 | Genotype:Wild Type | Treatment condition:GPI treatment | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 17 | Genotype:Wild Type | Treatment condition:control NP | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 17 | Genotype:Wild Type | Treatment condition:control NP | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 17 | Genotype:Wild Type | Treatment condition:control NP | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 17 | Genotype:Wild Type | Treatment condition:G1P LNP treatment | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 17 | Genotype:Wild Type | Treatment condition:G1P LNP treatment | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 17 | Genotype:Wild Type | Treatment condition:G1P LNP treatment | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 18 | Genotype:D421A | Treatment condition:G6PD D421 KI | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 18 | Genotype:D421A | Treatment condition:G6PD D421 KI | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 18 | Genotype:D421A | Treatment condition:G6PD D421 KI | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 18 | Genotype:Wild Type | Treatment condition:G6PD WT KI | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 18 | Genotype:Wild Type | Treatment condition:G6PD WT KI | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 18 | Genotype:Wild Type | Treatment condition:G6PD WT KI | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 19 | Genotype:Wild Type | Treatment condition:control treatment | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 19 | Genotype:Wild Type | Treatment condition:control treatment | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 19 | Genotype:Wild Type | Treatment condition:control treatment | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 19 | Genotype:Wild Type | Treatment condition:GPI treatment | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 19 | Genotype:Wild Type | Treatment condition:GPI treatment | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 19 | Genotype:Wild Type | Treatment condition:GPI treatment | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 20 | Genotype:Wild Type | Treatment condition:control treatment | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 20 | Genotype:Wild Type | Treatment condition:control treatment | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 20 | Genotype:Wild Type | Treatment condition:control treatment | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 20 | Genotype:Wild Type | Treatment condition:GPI treatment | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 20 | Genotype:Wild Type | Treatment condition:GPI treatment | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 20 | Genotype:Wild Type | Treatment condition:GPI treatment | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 21 | Genotype:Wild Type | Treatment condition:control treatment | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 21 | Genotype:Wild Type | Treatment condition:control treatment | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 21 | Genotype:Wild Type | Treatment condition:control treatment | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 21 | Genotype:Wild Type | Treatment condition:HEX treatment | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 21 | Genotype:Wild Type | Treatment condition:HEX treatment | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 21 | Genotype:Wild Type | Treatment condition:HEX treatment | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 21 | Genotype:Wild Type | Treatment condition:control treatment | Cell type:BMDM | Sample source:Immune cell	Experiment title:Experiment 21 | Genotype:Wild Type | Treatment condition:control treatment | Cell type:BMDM | Sample source:Immune cell	Experiment title:Experiment 21 | Genotype:Wild Type | Treatment condition:control treatment | Cell type:BMDM | Sample source:Immune cell	Experiment title:Experiment 21 | Genotype:Wild Type | Treatment condition:HEX treatment | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 21 | Genotype:Wild Type | Treatment condition:HEX treatment | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 21 | Genotype:Wild Type | Treatment condition:HEX treatment | Cell type:CD8 T cell | Sample source:Immune cell	Experiment title:Experiment 9 | Genotype:Wild Type | Treatment condition:control NP | Cell type:tumor-associated Macrophages | Sample source:Immune cell	Experiment title:Experiment 9 | Genotype:Wild Type | Treatment condition:control NP | Cell type:tumor-associated Macrophages | Sample source:Immune cell	Experiment title:Experiment 9 | Genotype:Wild Type | Treatment condition:control NP | Cell type:tumor-associated Macrophages | Sample source:Immune cell	Experiment title:Experiment 9 | Genotype:Wild Type | Treatment condition:G1P LNP treatment | Cell type:tumor-associated Macrophages | Sample source:Immune cell	Experiment title:Experiment 9 | Genotype:Wild Type | Treatment condition:G1P LNP treatment | Cell type:tumor-associated Macrophages | Sample source:Immune cell	Experiment title:Experiment 9 | Genotype:Wild Type | Treatment condition:G1P LNP treatment | Cell type:tumor-associated Macrophages | Sample source:Immune cell
ribose-5-phosphate M+0	2449023	3551253	3814118	1161484	2280524	1248977	28749251	27299923	34489153	8174440	11474817	18135193	235067	293889	347376	157291	291587	596784	532594	745765	578748	304133	322931	341865	446547	429526	499019	631778	639432	675243	541954	511730	552059	18079332	22587484	17394549	9249345	7716585	2693956	14839981	11588258	14209311	2120166	2658791	590130	10756728	9541608	1500657	51403655	60118166	12622656	5361154	6680897	8422395	980921	263647	46221823	49078799	41130933	12142738	7163157	21045615	11891441	1784281	2299219	4337757	5179609	4146668	0	0	0	288445	302564	324562	510353	478964	521456	420082	403256	468974	590130	602145	634578	211080	248419	227968	338269	325037	357628	499312	449864	507116	263882	252308	267630	7900401	1079799	8835035	2591990	1823300	2513403	6797633	17858495	13594304	1222979	25945714	42262551	3542513	4090132	4258317	989811	907171	870173	2815889	2265441	3315418	24371225	25138899	22827140	4824918	4476984	1229050	1155178	701998	4870378	12415455	18177804	20192480	14188787	3220187	20752955	45882254	29153837	29838822	0	0	0	0	0	0	1267525	3859253	3579264	28339059	23865761	14103179	29507947	3431099	2115303	2559807	6717447	4920474	0	0	0	0	0	0	23410773	94452334	27513238	2976280	11298873	1775934	78979625	95082128	99867376	13659342	1297099	3016235	24944223	48418705	48822786	2793092	2001109	7618435	4811497	5641651	5280454	4367332	4961878	9837893	
ribose-5-phosphate M+5	0	0	0	0	0	0	0	0	0	0	0	0	1358008	1246446	1417748	3385530	2912029	3916317	4542677	5786864	7062649	470562	433819	499789	4243409	3908843	4283610	5104068	5594000	4717950	680764	636629	641576	0	0	0	0	0	0	0	0	0	639127	946486	179456	7725110	7461667	771967	56567465	44986974	20999053	81070	212226	265271	0	0	30544929	25867581	32286955	1744649	1090429	4780357	12068230	2185543	6440954	66435	89538	54649	0	0	0	443576	405632	465896	639127	602563	598674	3983880	3654874	4025631	5099494	5214632	4452015	227772	258684	250512	3298579	3059019	3615219	4427765	4852407	4074410	395625	359878	430638	3554954	223018	2774496	338794	110284	231346	1805549	5762025	3793328	97110	3263896	5804089	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	196290	998246	766198	16309502	10407997	7176738	3060491	115123	71233	669041	1923509	2115463	0	0	0	0	0	0	27386068	161919863	52011839	90673	299493	0	74771582	54880429	68893940	2315532	210436	524868	22128323	31883018	33851033	114965	139768	119182	22078	60587	37169	53470	70724	167856	
sedoheptulose 7-phosphate	2908898	3104188	1010849	660668	774451	982029	5031577	4537220	10875319	4417839	1939931	2968721	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	3027115	5707978	6337644	2152489	1875917	574060	3250426	1696264	2758907	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	5356527	8637406	1363768	236974	390797	309054	6492643	3748022	4081566	5485793	11518909	7112498	1068790	3167805	5490069	1833208	249262	917109	6077160	3588887	5367581	4295862	13516587	7695605	73624784	51830326	43084848	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	
uridine diphosphate glucose M+6	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	360962	464781	351114	380012	302702	315719	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	
glucose 1-phosphate M+0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1234813	919949	961341	1082159	981077	846448	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	
glucose 1-phosphate M+6	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	80860	115981	173906	9108271	8465295	9976143	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	
glucose M+0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	23181346	28225573	30585379	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	
glucose M+1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	34533	50649	92089	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	
glucose M+2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	375266	359150	442031	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	
glucose M+3	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	660745	623909	658443	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	
glucose M+4	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1107381	1339908	1701362	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	
glucose M+5	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	11554988	13744427	11564197	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	
glucose M+6	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	191731303	202581803	184495334	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	
MS_METABOLITE_DATA_END
#METABOLITES
METABOLITES_START
metabolite_name	Pubchem ID	Kegg ID	m/z	rention time
ribose-5-phosphate M+0	77982	C00199	229.0107	5.2
ribose-5-phosphate M+5	77982	C00199	234.0257	5.2
sedoheptulose 7-phosphate	165007	C05382	289.0319	7.4
uridine diphosphate glucose M+6	8629	C00029	571.0646	7.2
glucose 1-phosphate M+0	65533	C00103	259.0213	7.4
glucose 1-phosphate M+6	65533	C00103	265.0393	7.4
glucose M+0	5793	C00031	179.055	4.9
glucose M+1	5793	C00031	180.058	4.9
glucose M+2	5793	C00031	181.061	4.9
glucose M+3	5793	C00031	182.064	4.9
glucose M+4	5793	C00031	183.067	4.9
glucose M+5	5793	C00031	184.07	4.9
glucose M+6	5793	C00031	185.073	4.9
METABOLITES_END
#END