#METABOLOMICS WORKBENCH Chris_Auger_20250503_050314 DATATRACK_ID:5881 STUDY_ID:ST003920 ANALYSIS_ID:AN006437 PROJECT_ID:PR002453
VERSION             	1
CREATED_ON             	May 16, 2025, 9:54 am
#PROJECT
PR:PROJECT_TITLE                 	Mitochondrial control of fuel switching through de novo carnitine synthesis
PR:PROJECT_SUMMARY               	Mitochondrial transport of trimethyllysine is the key first step in de novo
PR:PROJECT_SUMMARY               	biosynthesis of carnitine. In this study, we show that the uncharacterized
PR:PROJECT_SUMMARY               	transporter SLC25A45 mediates this step. Knockout of SLC25A45 leads to impaired
PR:PROJECT_SUMMARY               	fatty acid oxidation due to a decrease in carnitine. To demonstrate this,
PR:PROJECT_SUMMARY               	deuterated trimethyllysine (d9-TML) is traced in vitro (293 cells and primary
PR:PROJECT_SUMMARY               	hepatocytes) as well as subjected to mitochondrial uptake assays. MBP-SLC25A45
PR:PROJECT_SUMMARY               	in E. coli is used as a non-mammalian system to study trimethyllysine uptake. We
PR:PROJECT_SUMMARY               	further demonstrate a decrease in acylcarnitines via our lipidomic analysis,
PR:PROJECT_SUMMARY               	leading to decreased cold tolerance in vivo.
PR:INSTITUTE                     	BIDMC
PR:LAST_NAME                     	Auger
PR:FIRST_NAME                    	Christopher
PR:ADDRESS                       	3 Blackfan Street, Boston, MA, 02115, USA
PR:EMAIL                         	cauger1@bidmc.harvard.edu
PR:PHONE                         	8579282065
#STUDY
ST:STUDY_TITLE                   	Mitochondrial control of fuel switching through de novo carnitine synthesis
ST:STUDY_SUMMARY                 	Metabolic adaptation to fasting and cold exposure involves a dynamic fuel switch
ST:STUDY_SUMMARY                 	from glucose to fatty acid oxidation in peripheral organs, a process that
ST:STUDY_SUMMARY                 	depends on carnitine-mediated fatty acid oxidation in mitochondria. While
ST:STUDY_SUMMARY                 	dietary sources of animal origin (e.g., red meat) contribute to the carnitine
ST:STUDY_SUMMARY                 	pool, de novo synthesis from trimethyllysine (TML) is essential, particularly
ST:STUDY_SUMMARY                 	for those whose dietary sources are vegetables and fruits that contain
ST:STUDY_SUMMARY                 	negligible amounts of carnitine. However, the molecular pathway of de novo
ST:STUDY_SUMMARY                 	carnitine synthesis, in particular the mitochondrial transport step of TML,
ST:STUDY_SUMMARY                 	remains undefined. MBP-SLC25A45 in E. coli is used as a non-mammalian system to
ST:STUDY_SUMMARY                 	study trimethyllysine uptake. Here, we identify SLC25A45 as a previously
ST:STUDY_SUMMARY                 	uncharacterized mitochondrial TML carrier that is required for de novo carnitine
ST:STUDY_SUMMARY                 	biosynthesis. Genetic loss of SLC25A45 results in systemic carnitine and
ST:STUDY_SUMMARY                 	acylcarnitine deficiency, leading to impaired fatty acid oxidation and
ST:STUDY_SUMMARY                 	thermogenesis during cold adaptation. Unexpectedly, SLC25A45-deficient mice were
ST:STUDY_SUMMARY                 	somewhat resistant to the effects of a GLP1 receptor agonist (GLP-1RA) to lower
ST:STUDY_SUMMARY                 	food intake and maintained a high respiratory exchange ratio and low lipolysis
ST:STUDY_SUMMARY                 	following treatment with a GLP1 receptor agonist (GLP-1RA), rendering them
ST:STUDY_SUMMARY                 	resistant to GLP-1RA-induced adipose tissue loss. We further demonstrate a
ST:STUDY_SUMMARY                 	decrease in acylcarnitines via our lipidomic analysis, leading to decreased cold
ST:STUDY_SUMMARY                 	tolerance in vivo. Together, the present study uncovers mitochondrial metabolite
ST:STUDY_SUMMARY                 	transport as a key regulatory checkpoint in fuel switching during adaptation,
ST:STUDY_SUMMARY                 	with implications for systemic energy balance and response to GLP-1RA-mediated
ST:STUDY_SUMMARY                 	anti-obesity therapy.
ST:INSTITUTE                     	BIDMC
ST:LAST_NAME                     	Christopher
ST:FIRST_NAME                    	Auger
ST:ADDRESS                       	3 Blackfan Street, 02115, Boston, MA
ST:EMAIL                         	cauger1@bidmc.harvard.edu
ST:PHONE                         	8579282065
#SUBJECT
SU:SUBJECT_TYPE                  	Mammal
SU:SUBJECT_SPECIES               	Mus musculus
SU:TAXONOMY_ID                   	10090
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data
SUBJECT_SAMPLE_FACTORS           	E1	E1P	Sample source:Ing Mito | Sample type:Ing Mito | Genotype:Ctl | Treatment:10 uM D9-TML	Batch=B1; RAW_FILE_NAME(Raw_file_name)=E1_pos.raw
SUBJECT_SAMPLE_FACTORS           	E2	E2P	Sample source:Ing Mito | Sample type:Ing Mito | Genotype:Ctl | Treatment:10 uM D9-TML	Batch=B1; RAW_FILE_NAME(Raw_file_name)=E2_pos.raw
SUBJECT_SAMPLE_FACTORS           	E3	E3P	Sample source:Ing Mito | Sample type:Ing Mito | Genotype:Ctl | Treatment:10 uM D9-TML	Batch=B1; RAW_FILE_NAME(Raw_file_name)=E3_pos.raw
SUBJECT_SAMPLE_FACTORS           	E4	E4P	Sample source:Ing Mito | Sample type:Ing Mito | Genotype:Ctl | Treatment:10 uM D9-TML	Batch=B1; RAW_FILE_NAME(Raw_file_name)=E4_pos.raw
SUBJECT_SAMPLE_FACTORS           	C1	C1P	Sample source:Ing Mito | Sample type:Ing Mito | Genotype:Cre | Treatment:10 uM D9-TML	Batch=B1; RAW_FILE_NAME(Raw_file_name)=C1_pos.raw
SUBJECT_SAMPLE_FACTORS           	C2	C2P	Sample source:Ing Mito | Sample type:Ing Mito | Genotype:Cre | Treatment:10 uM D9-TML	Batch=B1; RAW_FILE_NAME(Raw_file_name)=C2_pos.raw
SUBJECT_SAMPLE_FACTORS           	C3	C3P	Sample source:Ing Mito | Sample type:Ing Mito | Genotype:Cre | Treatment:10 uM D9-TML	Batch=B1; RAW_FILE_NAME(Raw_file_name)=C3_pos.raw
SUBJECT_SAMPLE_FACTORS           	C4	C4P	Sample source:Ing Mito | Sample type:Ing Mito | Genotype:Cre | Treatment:10 uM D9-TML	Batch=B1; RAW_FILE_NAME(Raw_file_name)=C4_pos.raw
SUBJECT_SAMPLE_FACTORS           	KO1	2K1	Sample source:293 Mito | Sample type:293 Mito | Genotype:KO | Treatment:10 uM D9-TML	Batch=B1; RAW_FILE_NAME(Raw_file_name)=2K1_pos.raw
SUBJECT_SAMPLE_FACTORS           	KO2	2K2	Sample source:293 Mito | Sample type:293 Mito | Genotype:KO | Treatment:10 uM D9-TML	Batch=B1; RAW_FILE_NAME(Raw_file_name)=2K2_pos.raw
SUBJECT_SAMPLE_FACTORS           	KO3	2K3	Sample source:293 Mito | Sample type:293 Mito | Genotype:KO | Treatment:10 uM D9-TML	Batch=B1; RAW_FILE_NAME(Raw_file_name)=2K3_pos.raw
SUBJECT_SAMPLE_FACTORS           	KO4	2K4	Sample source:293 Mito | Sample type:293 Mito | Genotype:KO | Treatment:10 uM D9-TML	Batch=B1; RAW_FILE_NAME(Raw_file_name)=2K4_pos.raw
SUBJECT_SAMPLE_FACTORS           	Rescue1	2R1	Sample source:293 Mito | Sample type:293 Mito | Genotype:Rescue | Treatment:10 uM D9-TML	Batch=B1; RAW_FILE_NAME(Raw_file_name)=2R1_pos.raw
SUBJECT_SAMPLE_FACTORS           	Rescue2	2R2	Sample source:293 Mito | Sample type:293 Mito | Genotype:Rescue | Treatment:10 uM D9-TML	Batch=B1; RAW_FILE_NAME(Raw_file_name)=2R2_pos.raw
SUBJECT_SAMPLE_FACTORS           	Rescue3	2R3	Sample source:293 Mito | Sample type:293 Mito | Genotype:Rescue | Treatment:10 uM D9-TML	Batch=B1; RAW_FILE_NAME(Raw_file_name)=2R3_pos.raw
SUBJECT_SAMPLE_FACTORS           	Rescue4	2R4	Sample source:293 Mito | Sample type:293 Mito | Genotype:Rescue | Treatment:10 uM D9-TML	Batch=B1; RAW_FILE_NAME(Raw_file_name)=2R4_pos.raw
SUBJECT_SAMPLE_FACTORS           	WT1	S10	Sample source:Hepatocytes | Sample type:Hepatocytes | Genotype:Control | Treatment:10 uM D9-TML	Batch=B2; RAW_FILE_NAME(Raw_file_name)=S10_scanevent2.mzXML
SUBJECT_SAMPLE_FACTORS           	WT1	S13	Sample source:Hepatocytes | Sample type:Hepatocytes | Genotype:Control | Treatment:10 uM D9-TML	Batch=B2; RAW_FILE_NAME(Raw_file_name)=S13_scanevent2.mzXML
SUBJECT_SAMPLE_FACTORS           	WT1	S16	Sample source:Hepatocytes | Sample type:Hepatocytes | Genotype:Control | Treatment:10 uM D9-TML	Batch=B2; RAW_FILE_NAME(Raw_file_name)=S16_scanevent2.mzXML
SUBJECT_SAMPLE_FACTORS           	WT2	S11	Sample source:Hepatocytes | Sample type:Hepatocytes | Genotype:Control | Treatment:10 uM D9-TML	Batch=B2; RAW_FILE_NAME(Raw_file_name)=S11_scanevent2.mzXML
SUBJECT_SAMPLE_FACTORS           	WT2	S14	Sample source:Hepatocytes | Sample type:Hepatocytes | Genotype:Control | Treatment:10 uM D9-TML	Batch=B2; RAW_FILE_NAME(Raw_file_name)=S14_scanevent2.mzXML
SUBJECT_SAMPLE_FACTORS           	WT2	S17	Sample source:Hepatocytes | Sample type:Hepatocytes | Genotype:Control | Treatment:10 uM D9-TML	Batch=B2; RAW_FILE_NAME(Raw_file_name)=S17_scanevent2.mzXML
SUBJECT_SAMPLE_FACTORS           	WT3	S12	Sample source:Hepatocytes | Sample type:Hepatocytes | Genotype:Control | Treatment:10 uM D9-TML	Batch=B2; RAW_FILE_NAME(Raw_file_name)=S12_scanevent2.mzXML
SUBJECT_SAMPLE_FACTORS           	WT3	S15	Sample source:Hepatocytes | Sample type:Hepatocytes | Genotype:Control | Treatment:10 uM D9-TML	Batch=B2; RAW_FILE_NAME(Raw_file_name)=S15_scanevent2.mzXML
SUBJECT_SAMPLE_FACTORS           	WT3	S18	Sample source:Hepatocytes | Sample type:Hepatocytes | Genotype:Control | Treatment:10 uM D9-TML	Batch=B2; RAW_FILE_NAME(Raw_file_name)=S18_scanevent2.mzXML
SUBJECT_SAMPLE_FACTORS           	45KO1	S28	Sample source:Hepatocytes | Sample type:Hepatocytes | Genotype:KO | Treatment:10 uM D9-TML	Batch=B2; RAW_FILE_NAME(Raw_file_name)=S28_scanevent2.mzXML
SUBJECT_SAMPLE_FACTORS           	45KO1	S31	Sample source:Hepatocytes | Sample type:Hepatocytes | Genotype:KO | Treatment:10 uM D9-TML	Batch=B2; RAW_FILE_NAME(Raw_file_name)=S31_scanevent2.mzXML
SUBJECT_SAMPLE_FACTORS           	45KO1	S34	Sample source:Hepatocytes | Sample type:Hepatocytes | Genotype:KO | Treatment:10 uM D9-TML	Batch=B2; RAW_FILE_NAME(Raw_file_name)=S34_scanevent2.mzXML
SUBJECT_SAMPLE_FACTORS           	45KO2	S29	Sample source:Hepatocytes | Sample type:Hepatocytes | Genotype:KO | Treatment:10 uM D9-TML	Batch=B2; RAW_FILE_NAME(Raw_file_name)=S29_scanevent2.mzXML
SUBJECT_SAMPLE_FACTORS           	45KO2	S32	Sample source:Hepatocytes | Sample type:Hepatocytes | Genotype:KO | Treatment:10 uM D9-TML	Batch=B2; RAW_FILE_NAME(Raw_file_name)=S32_scanevent2.mzXML
SUBJECT_SAMPLE_FACTORS           	45KO2	S35	Sample source:Hepatocytes | Sample type:Hepatocytes | Genotype:KO | Treatment:10 uM D9-TML	Batch=B2; RAW_FILE_NAME(Raw_file_name)=S35_scanevent2.mzXML
SUBJECT_SAMPLE_FACTORS           	45KO3	S30	Sample source:Hepatocytes | Sample type:Hepatocytes | Genotype:KO | Treatment:10 uM D9-TML	Batch=B2; RAW_FILE_NAME(Raw_file_name)=S30_scanevent2.mzXML
SUBJECT_SAMPLE_FACTORS           	45KO3	S33	Sample source:Hepatocytes | Sample type:Hepatocytes | Genotype:KO | Treatment:10 uM D9-TML	Batch=B2; RAW_FILE_NAME(Raw_file_name)=S33_scanevent2.mzXML
SUBJECT_SAMPLE_FACTORS           	45KO3	S36	Sample source:Hepatocytes | Sample type:Hepatocytes | Genotype:KO | Treatment:10 uM D9-TML	Batch=B2; RAW_FILE_NAME(Raw_file_name)=S36_scanevent2.mzXML
SUBJECT_SAMPLE_FACTORS           	Emp1	pSamp41	Sample source:Ecoli | Sample type:Ecoli | Genotype:Control | Treatment:10 uM D9-TML	Batch=B3; RAW_FILE_NAME(Raw_file_name)=pSamp41.raw
SUBJECT_SAMPLE_FACTORS           	Emp2	pSamp42	Sample source:Ecoli | Sample type:Ecoli | Genotype:Control | Treatment:10 uM D9-TML	Batch=B3; RAW_FILE_NAME(Raw_file_name)=pSamp42.raw
SUBJECT_SAMPLE_FACTORS           	Emp3	pSamp43	Sample source:Ecoli | Sample type:Ecoli | Genotype:Control | Treatment:10 uM D9-TML	Batch=B3; RAW_FILE_NAME(Raw_file_name)=pSamp43.raw
SUBJECT_SAMPLE_FACTORS           	Emp4	pSamp44	Sample source:Ecoli | Sample type:Ecoli | Genotype:Control | Treatment:10 uM D9-TML	Batch=B3; RAW_FILE_NAME(Raw_file_name)=pSamp44.raw
SUBJECT_SAMPLE_FACTORS           	Emp1	pSamp49	Sample source:Ecoli | Sample type:Ecoli | Genotype:Control | Treatment:10 uM D9-TML	Batch=B3; RAW_FILE_NAME(Raw_file_name)=pSamp49.raw
SUBJECT_SAMPLE_FACTORS           	Emp2	pSamp50	Sample source:Ecoli | Sample type:Ecoli | Genotype:Control | Treatment:10 uM D9-TML	Batch=B3; RAW_FILE_NAME(Raw_file_name)=pSamp50.raw
SUBJECT_SAMPLE_FACTORS           	Emp3	pSamp51	Sample source:Ecoli | Sample type:Ecoli | Genotype:Control | Treatment:10 uM D9-TML	Batch=B3; RAW_FILE_NAME(Raw_file_name)=pSamp51.raw
SUBJECT_SAMPLE_FACTORS           	Emp4	pSamp52	Sample source:Ecoli | Sample type:Ecoli | Genotype:Control | Treatment:10 uM D9-TML	Batch=B3; RAW_FILE_NAME(Raw_file_name)=pSamp52.raw
SUBJECT_SAMPLE_FACTORS           	Emp1	pSamp57	Sample source:Ecoli | Sample type:Ecoli | Genotype:Control | Treatment:10 uM D9-TML	Batch=B3; RAW_FILE_NAME(Raw_file_name)=pSamp57.raw
SUBJECT_SAMPLE_FACTORS           	Emp2	pSamp58	Sample source:Ecoli | Sample type:Ecoli | Genotype:Control | Treatment:10 uM D9-TML	Batch=B3; RAW_FILE_NAME(Raw_file_name)=pSamp58.raw
SUBJECT_SAMPLE_FACTORS           	Emp3	pSamp59	Sample source:Ecoli | Sample type:Ecoli | Genotype:Control | Treatment:10 uM D9-TML	Batch=B3; RAW_FILE_NAME(Raw_file_name)=pSamp59.raw
SUBJECT_SAMPLE_FACTORS           	Emp4	pSamp60	Sample source:Ecoli | Sample type:Ecoli | Genotype:Control | Treatment:10 uM D9-TML	Batch=B3; RAW_FILE_NAME(Raw_file_name)=pSamp60.raw
SUBJECT_SAMPLE_FACTORS           	Emp1	pSamp65	Sample source:Ecoli | Sample type:Ecoli | Genotype:Control | Treatment:10 uM D9-TML	Batch=B3; RAW_FILE_NAME(Raw_file_name)=pSamp65.raw
SUBJECT_SAMPLE_FACTORS           	Emp2	pSamp66	Sample source:Ecoli | Sample type:Ecoli | Genotype:Control | Treatment:10 uM D9-TML	Batch=B3; RAW_FILE_NAME(Raw_file_name)=pSamp66.raw
SUBJECT_SAMPLE_FACTORS           	Emp3	pSamp67	Sample source:Ecoli | Sample type:Ecoli | Genotype:Control | Treatment:10 uM D9-TML	Batch=B3; RAW_FILE_NAME(Raw_file_name)=pSamp67.raw
SUBJECT_SAMPLE_FACTORS           	Emp4	pSamp68	Sample source:Ecoli | Sample type:Ecoli | Genotype:Control | Treatment:10 uM D9-TML	Batch=B3; RAW_FILE_NAME(Raw_file_name)=pSamp68.raw
SUBJECT_SAMPLE_FACTORS           	Emp1	pSamp73	Sample source:Ecoli | Sample type:Ecoli | Genotype:Control | Treatment:10 uM D9-TML	Batch=B3; RAW_FILE_NAME(Raw_file_name)=pSamp73.raw
SUBJECT_SAMPLE_FACTORS           	Emp2	pSamp74	Sample source:Ecoli | Sample type:Ecoli | Genotype:Control | Treatment:10 uM D9-TML	Batch=B3; RAW_FILE_NAME(Raw_file_name)=pSamp74.raw
SUBJECT_SAMPLE_FACTORS           	Emp3	pSamp75	Sample source:Ecoli | Sample type:Ecoli | Genotype:Control | Treatment:10 uM D9-TML	Batch=B3; RAW_FILE_NAME(Raw_file_name)=pSamp75.raw
SUBJECT_SAMPLE_FACTORS           	Emp4	pSamp76	Sample source:Ecoli | Sample type:Ecoli | Genotype:Control | Treatment:10 uM D9-TML	Batch=B3; RAW_FILE_NAME(Raw_file_name)=pSamp76.raw
SUBJECT_SAMPLE_FACTORS           	MBP451	pSamp45	Sample source:Ecoli | Sample type:Ecoli | Genotype:Overexpression | Treatment:10 uM D9-TML	Batch=B3; RAW_FILE_NAME(Raw_file_name)=pSamp45.raw
SUBJECT_SAMPLE_FACTORS           	MBP452	pSamp46	Sample source:Ecoli | Sample type:Ecoli | Genotype:Overexpression | Treatment:10 uM D9-TML	Batch=B3; RAW_FILE_NAME(Raw_file_name)=pSamp46.raw
SUBJECT_SAMPLE_FACTORS           	MBP453	pSamp47	Sample source:Ecoli | Sample type:Ecoli | Genotype:Overexpression | Treatment:10 uM D9-TML	Batch=B3; RAW_FILE_NAME(Raw_file_name)=pSamp47.raw
SUBJECT_SAMPLE_FACTORS           	MBP454	pSamp48	Sample source:Ecoli | Sample type:Ecoli | Genotype:Overexpression | Treatment:10 uM D9-TML	Batch=B3; RAW_FILE_NAME(Raw_file_name)=pSamp48.raw
SUBJECT_SAMPLE_FACTORS           	MBP451	pSamp53	Sample source:Ecoli | Sample type:Ecoli | Genotype:Overexpression | Treatment:10 uM D9-TML	Batch=B3; RAW_FILE_NAME(Raw_file_name)=pSamp53.raw
SUBJECT_SAMPLE_FACTORS           	MBP452	pSamp54	Sample source:Ecoli | Sample type:Ecoli | Genotype:Overexpression | Treatment:10 uM D9-TML	Batch=B3; RAW_FILE_NAME(Raw_file_name)=pSamp54.raw
SUBJECT_SAMPLE_FACTORS           	MBP453	pSamp55	Sample source:Ecoli | Sample type:Ecoli | Genotype:Overexpression | Treatment:10 uM D9-TML	Batch=B3; RAW_FILE_NAME(Raw_file_name)=pSamp55.raw
SUBJECT_SAMPLE_FACTORS           	MBP454	pSamp56	Sample source:Ecoli | Sample type:Ecoli | Genotype:Overexpression | Treatment:10 uM D9-TML	Batch=B3; RAW_FILE_NAME(Raw_file_name)=pSamp56.raw
SUBJECT_SAMPLE_FACTORS           	MBP451	pSamp61	Sample source:Ecoli | Sample type:Ecoli | Genotype:Overexpression | Treatment:10 uM D9-TML	Batch=B3; RAW_FILE_NAME(Raw_file_name)=pSamp61.raw
SUBJECT_SAMPLE_FACTORS           	MBP452	pSamp62	Sample source:Ecoli | Sample type:Ecoli | Genotype:Overexpression | Treatment:10 uM D9-TML	Batch=B3; RAW_FILE_NAME(Raw_file_name)=pSamp62.raw
SUBJECT_SAMPLE_FACTORS           	MBP453	pSamp63	Sample source:Ecoli | Sample type:Ecoli | Genotype:Overexpression | Treatment:10 uM D9-TML	Batch=B3; RAW_FILE_NAME(Raw_file_name)=pSamp63.raw
SUBJECT_SAMPLE_FACTORS           	MBP454	pSamp64	Sample source:Ecoli | Sample type:Ecoli | Genotype:Overexpression | Treatment:10 uM D9-TML	Batch=B3; RAW_FILE_NAME(Raw_file_name)=pSamp64.raw
SUBJECT_SAMPLE_FACTORS           	MBP451	pSamp69	Sample source:Ecoli | Sample type:Ecoli | Genotype:Overexpression | Treatment:10 uM D9-TML	Batch=B3; RAW_FILE_NAME(Raw_file_name)=pSamp69.raw
SUBJECT_SAMPLE_FACTORS           	MBP452	pSamp70	Sample source:Ecoli | Sample type:Ecoli | Genotype:Overexpression | Treatment:10 uM D9-TML	Batch=B3; RAW_FILE_NAME(Raw_file_name)=pSamp70.raw
SUBJECT_SAMPLE_FACTORS           	MBP453	pSamp71	Sample source:Ecoli | Sample type:Ecoli | Genotype:Overexpression | Treatment:10 uM D9-TML	Batch=B3; RAW_FILE_NAME(Raw_file_name)=pSamp71.raw
SUBJECT_SAMPLE_FACTORS           	MBP454	pSamp72	Sample source:Ecoli | Sample type:Ecoli | Genotype:Overexpression | Treatment:10 uM D9-TML	Batch=B3; RAW_FILE_NAME(Raw_file_name)=pSamp72.raw
SUBJECT_SAMPLE_FACTORS           	MBP451	pSamp77	Sample source:Ecoli | Sample type:Ecoli | Genotype:Overexpression | Treatment:10 uM D9-TML	Batch=B3; RAW_FILE_NAME(Raw_file_name)=pSamp77.raw
SUBJECT_SAMPLE_FACTORS           	MBP452	pSamp78	Sample source:Ecoli | Sample type:Ecoli | Genotype:Overexpression | Treatment:10 uM D9-TML	Batch=B3; RAW_FILE_NAME(Raw_file_name)=pSamp78.raw
SUBJECT_SAMPLE_FACTORS           	MBP453	pSamp79	Sample source:Ecoli | Sample type:Ecoli | Genotype:Overexpression | Treatment:10 uM D9-TML	Batch=B3; RAW_FILE_NAME(Raw_file_name)=pSamp79.raw
SUBJECT_SAMPLE_FACTORS           	MBP454	pSamp80	Sample source:Ecoli | Sample type:Ecoli | Genotype:Overexpression | Treatment:10 uM D9-TML	Batch=B3; RAW_FILE_NAME(Raw_file_name)=pSamp80.raw
SUBJECT_SAMPLE_FACTORS           	Emp1	S40	Sample source:Ecoli | Sample type:Ecoli | Genotype:Control | Treatment:10 uM D9-TML	Batch=B4; RAW_FILE_NAME(Raw_file_name)=S40_scanevent2.mzXML
SUBJECT_SAMPLE_FACTORS           	Emp2	S41	Sample source:Ecoli | Sample type:Ecoli | Genotype:Control | Treatment:10 uM D9-TML	Batch=B4; RAW_FILE_NAME(Raw_file_name)=S41_scanevent2.mzXML
SUBJECT_SAMPLE_FACTORS           	Emp3	S42	Sample source:Ecoli | Sample type:Ecoli | Genotype:Control | Treatment:10 uM D9-TML	Batch=B4; RAW_FILE_NAME(Raw_file_name)=S42_scanevent2.mzXML
SUBJECT_SAMPLE_FACTORS           	Emp4	S43	Sample source:Ecoli | Sample type:Ecoli | Genotype:Control | Treatment:10 uM D9-TML	Batch=B4; RAW_FILE_NAME(Raw_file_name)=S43_scanevent2.mzXML
SUBJECT_SAMPLE_FACTORS           	Emp5	S44	Sample source:Ecoli | Sample type:Ecoli | Genotype:Control | Treatment:10 uM D9-TML	Batch=B4; RAW_FILE_NAME(Raw_file_name)=S44_scanevent2.mzXML
SUBJECT_SAMPLE_FACTORS           	MBP451	S45	Sample source:Ecoli | Sample type:Ecoli | Genotype:Overexpression | Treatment:10 uM D9-TML	Batch=B4; RAW_FILE_NAME(Raw_file_name)=S45_scanevent2.mzXML
SUBJECT_SAMPLE_FACTORS           	MBP452	S46	Sample source:Ecoli | Sample type:Ecoli | Genotype:Overexpression | Treatment:10 uM D9-TML	Batch=B4; RAW_FILE_NAME(Raw_file_name)=S46_scanevent2.mzXML
SUBJECT_SAMPLE_FACTORS           	MBP453	S47	Sample source:Ecoli | Sample type:Ecoli | Genotype:Overexpression | Treatment:10 uM D9-TML	Batch=B4; RAW_FILE_NAME(Raw_file_name)=S47_scanevent2.mzXML
SUBJECT_SAMPLE_FACTORS           	MBP454	S48	Sample source:Ecoli | Sample type:Ecoli | Genotype:Overexpression | Treatment:10 uM D9-TML	Batch=B4; RAW_FILE_NAME(Raw_file_name)=S48_scanevent2.mzXML
SUBJECT_SAMPLE_FACTORS           	MBP455	S49	Sample source:Ecoli | Sample type:Ecoli | Genotype:Overexpression | Treatment:10 uM D9-TML	Batch=B4; RAW_FILE_NAME(Raw_file_name)=S49_scanevent2.mzXML
SUBJECT_SAMPLE_FACTORS           	Control1	S50	Sample source:Serum | Sample type:Serum | Genotype:Control | Treatment:None	Batch=B5; RAW_FILE_NAME(Raw_file_name)=S50_scanevent2.mzXML
SUBJECT_SAMPLE_FACTORS           	Control2	S51	Sample source:Serum | Sample type:Serum | Genotype:Control | Treatment:None	Batch=B5; RAW_FILE_NAME(Raw_file_name)=S51_scanevent2.mzXML
SUBJECT_SAMPLE_FACTORS           	Control3	S52	Sample source:Serum | Sample type:Serum | Genotype:Control | Treatment:None	Batch=B5; RAW_FILE_NAME(Raw_file_name)=S52_scanevent2.mzXML
SUBJECT_SAMPLE_FACTORS           	Control4	S53	Sample source:Serum | Sample type:Serum | Genotype:Control | Treatment:None	Batch=B5; RAW_FILE_NAME(Raw_file_name)=S53_scanevent2.mzXML
SUBJECT_SAMPLE_FACTORS           	Control5	S54	Sample source:Serum | Sample type:Serum | Genotype:Control | Treatment:None	Batch=B5; RAW_FILE_NAME(Raw_file_name)=S54_scanevent2.mzXML
SUBJECT_SAMPLE_FACTORS           	45KO1	S55	Sample source:Serum | Sample type:Serum | Genotype:KO | Treatment:None	Batch=B5; RAW_FILE_NAME(Raw_file_name)=S55_scanevent2.mzXML
SUBJECT_SAMPLE_FACTORS           	45KO2	S56	Sample source:Serum | Sample type:Serum | Genotype:KO | Treatment:None	Batch=B5; RAW_FILE_NAME(Raw_file_name)=S56_scanevent2.mzXML
SUBJECT_SAMPLE_FACTORS           	45KO3	S57	Sample source:Serum | Sample type:Serum | Genotype:KO | Treatment:None	Batch=B5; RAW_FILE_NAME(Raw_file_name)=S57_scanevent2.mzXML
SUBJECT_SAMPLE_FACTORS           	45KO4	S58	Sample source:Serum | Sample type:Serum | Genotype:KO | Treatment:None	Batch=B5; RAW_FILE_NAME(Raw_file_name)=S58_scanevent2.mzXML
SUBJECT_SAMPLE_FACTORS           	45KO5	S59	Sample source:Serum | Sample type:Serum | Genotype:KO | Treatment:None	Batch=B5; RAW_FILE_NAME(Raw_file_name)=S59_scanevent2.mzXML
SUBJECT_SAMPLE_FACTORS           	WT1	S60	Sample source:293 cells | Sample type:293 cells | Genotype:Control | Treatment:10 uM D9-TML	Batch=B6; RAW_FILE_NAME(Raw_file_name)=S60_scanevent2.mzXML
SUBJECT_SAMPLE_FACTORS           	WT2	S61	Sample source:293 cells | Sample type:293 cells | Genotype:Control | Treatment:10 uM D9-TML	Batch=B6; RAW_FILE_NAME(Raw_file_name)=S61_scanevent2.mzXML
SUBJECT_SAMPLE_FACTORS           	WT3	S62	Sample source:293 cells | Sample type:293 cells | Genotype:Control | Treatment:10 uM D9-TML	Batch=B6; RAW_FILE_NAME(Raw_file_name)=S62_scanevent2.mzXML
SUBJECT_SAMPLE_FACTORS           	WT4	S63	Sample source:293 cells | Sample type:293 cells | Genotype:Control | Treatment:10 uM D9-TML	Batch=B6; RAW_FILE_NAME(Raw_file_name)=S63_scanevent2.mzXML
SUBJECT_SAMPLE_FACTORS           	WT5	S64	Sample source:293 cells | Sample type:293 cells | Genotype:Control | Treatment:10 uM D9-TML	Batch=B6; RAW_FILE_NAME(Raw_file_name)=S64_scanevent2.mzXML
SUBJECT_SAMPLE_FACTORS           	cDNA1	S65	Sample source:293 cells | Sample type:293 cells | Genotype:Overexpression | Treatment:10 uM D9-TML	Batch=B6; RAW_FILE_NAME(Raw_file_name)=S65_scanevent2.mzXML
SUBJECT_SAMPLE_FACTORS           	cDNA2	S66	Sample source:293 cells | Sample type:293 cells | Genotype:Overexpression | Treatment:10 uM D9-TML	Batch=B6; RAW_FILE_NAME(Raw_file_name)=S66_scanevent2.mzXML
SUBJECT_SAMPLE_FACTORS           	cDNA3	S67	Sample source:293 cells | Sample type:293 cells | Genotype:Overexpression | Treatment:10 uM D9-TML	Batch=B6; RAW_FILE_NAME(Raw_file_name)=S67_scanevent2.mzXML
SUBJECT_SAMPLE_FACTORS           	cDNA4	S68	Sample source:293 cells | Sample type:293 cells | Genotype:Overexpression | Treatment:10 uM D9-TML	Batch=B6; RAW_FILE_NAME(Raw_file_name)=S68_scanevent2.mzXML
SUBJECT_SAMPLE_FACTORS           	cDNA5	S69	Sample source:293 cells | Sample type:293 cells | Genotype:Overexpression | Treatment:10 uM D9-TML	Batch=B6; RAW_FILE_NAME(Raw_file_name)=S69_scanevent2.mzXML
SUBJECT_SAMPLE_FACTORS           	Control1	plasma_ctl1	Sample source:Serum | Sample type:Serum | Genotype:Control | Treatment:72h 6C	Batch=B7; RAW_FILE_NAME(Raw_file_name)=plasma_ctl1_pos.raw
SUBJECT_SAMPLE_FACTORS           	Control2	plasma_ctl2	Sample source:Serum | Sample type:Serum | Genotype:Control | Treatment:72h 6C	Batch=B7; RAW_FILE_NAME(Raw_file_name)=plasma_ctl2_pos.raw
SUBJECT_SAMPLE_FACTORS           	Control3	plasma_ctl3	Sample source:Serum | Sample type:Serum | Genotype:Control | Treatment:72h 6C	Batch=B7; RAW_FILE_NAME(Raw_file_name)=plasma_ctl3_pos.raw
SUBJECT_SAMPLE_FACTORS           	Control4	plasma_ctl4	Sample source:Serum | Sample type:Serum | Genotype:Control | Treatment:72h 6C	Batch=B7; RAW_FILE_NAME(Raw_file_name)=plasma_ctl4_pos.raw
SUBJECT_SAMPLE_FACTORS           	Control5	plasma_ctl5	Sample source:Serum | Sample type:Serum | Genotype:Control | Treatment:72h 6C	Batch=B7; RAW_FILE_NAME(Raw_file_name)=plasma_ctl5_pos.raw
SUBJECT_SAMPLE_FACTORS           	Control6	plasma_ctl6	Sample source:Serum | Sample type:Serum | Genotype:Control | Treatment:72h 6C	Batch=B7; RAW_FILE_NAME(Raw_file_name)=plasma_ctl6_pos.raw
SUBJECT_SAMPLE_FACTORS           	Control7	plasma_ctl7	Sample source:Serum | Sample type:Serum | Genotype:Control | Treatment:72h 6C	Batch=B7; RAW_FILE_NAME(Raw_file_name)=plasma_ctl7_pos.raw
SUBJECT_SAMPLE_FACTORS           	45KO1	plasma_ko1	Sample source:Serum | Sample type:Serum | Genotype:KO | Treatment:72h 6C	Batch=B7; RAW_FILE_NAME(Raw_file_name)=plasma_ko1_pos.raw
SUBJECT_SAMPLE_FACTORS           	45KO2	plasma_ko2	Sample source:Serum | Sample type:Serum | Genotype:KO | Treatment:72h 6C	Batch=B7; RAW_FILE_NAME(Raw_file_name)=plasma_ko2_pos.raw
SUBJECT_SAMPLE_FACTORS           	45KO3	plasma_ko3	Sample source:Serum | Sample type:Serum | Genotype:KO | Treatment:72h 6C	Batch=B7; RAW_FILE_NAME(Raw_file_name)=plasma_ko3_pos.raw
SUBJECT_SAMPLE_FACTORS           	45KO4	plasma_ko4	Sample source:Serum | Sample type:Serum | Genotype:KO | Treatment:72h 6C	Batch=B7; RAW_FILE_NAME(Raw_file_name)=plasma_ko4_pos.raw
SUBJECT_SAMPLE_FACTORS           	45KO5	plasma_ko5	Sample source:Serum | Sample type:Serum | Genotype:KO | Treatment:72h 6C	Batch=B7; RAW_FILE_NAME(Raw_file_name)=plasma_ko5_pos.raw
SUBJECT_SAMPLE_FACTORS           	45KO6	plasma_ko6	Sample source:Serum | Sample type:Serum | Genotype:KO | Treatment:72h 6C	Batch=B7; RAW_FILE_NAME(Raw_file_name)=plasma_ko6_pos.raw
SUBJECT_SAMPLE_FACTORS           	45KO7	plasma_ko7	Sample source:Serum | Sample type:Serum | Genotype:KO | Treatment:72h 6C	Batch=B7; RAW_FILE_NAME(Raw_file_name)=plasma_ko7_pos.raw
SUBJECT_SAMPLE_FACTORS           	Control1	Pos_1	Sample source:Serum | Sample type:Serum | Genotype:Control | Treatment:72h 6C	Batch=B8; RAW_FILE_NAME(Raw_file_name)=Pos_1.raw
SUBJECT_SAMPLE_FACTORS           	Control2	Pos_2	Sample source:Serum | Sample type:Serum | Genotype:Control | Treatment:72h 6C	Batch=B8; RAW_FILE_NAME(Raw_file_name)=Pos_2.raw
SUBJECT_SAMPLE_FACTORS           	Control3	Pos_3	Sample source:Serum | Sample type:Serum | Genotype:Control | Treatment:72h 6C	Batch=B8; RAW_FILE_NAME(Raw_file_name)=Pos_3.raw
SUBJECT_SAMPLE_FACTORS           	Control4	Pos_4	Sample source:Serum | Sample type:Serum | Genotype:Control | Treatment:72h 6C	Batch=B8; RAW_FILE_NAME(Raw_file_name)=Pos_4.raw
SUBJECT_SAMPLE_FACTORS           	Control5	Pos_5	Sample source:Serum | Sample type:Serum | Genotype:Control | Treatment:72h 6C	Batch=B8; RAW_FILE_NAME(Raw_file_name)=Pos_5.raw
SUBJECT_SAMPLE_FACTORS           	Control6	Pos_6	Sample source:Serum | Sample type:Serum | Genotype:Control | Treatment:72h 6C	Batch=B8; RAW_FILE_NAME(Raw_file_name)=Pos_6.raw
SUBJECT_SAMPLE_FACTORS           	Control7	Pos_7	Sample source:Serum | Sample type:Serum | Genotype:Control | Treatment:72h 6C	Batch=B8; RAW_FILE_NAME(Raw_file_name)=Pos_7.raw
SUBJECT_SAMPLE_FACTORS           	45KO1	Pos_8	Sample source:Serum | Sample type:Serum | Genotype:KO | Treatment:72h 6C	Batch=B8; RAW_FILE_NAME(Raw_file_name)=Pos_8.raw
SUBJECT_SAMPLE_FACTORS           	45KO2	Pos_9	Sample source:Serum | Sample type:Serum | Genotype:KO | Treatment:72h 6C	Batch=B8; RAW_FILE_NAME(Raw_file_name)=Pos_9.raw
SUBJECT_SAMPLE_FACTORS           	45KO3	Pos_10	Sample source:Serum | Sample type:Serum | Genotype:KO | Treatment:72h 6C	Batch=B8; RAW_FILE_NAME(Raw_file_name)=Pos_10.raw
SUBJECT_SAMPLE_FACTORS           	45KO4	Pos_11	Sample source:Serum | Sample type:Serum | Genotype:KO | Treatment:72h 6C	Batch=B8; RAW_FILE_NAME(Raw_file_name)=Pos_11.raw
SUBJECT_SAMPLE_FACTORS           	45KO5	Pos_12	Sample source:Serum | Sample type:Serum | Genotype:KO | Treatment:72h 6C	Batch=B8; RAW_FILE_NAME(Raw_file_name)=Pos_12.raw
SUBJECT_SAMPLE_FACTORS           	45KO6	Pos_13	Sample source:Serum | Sample type:Serum | Genotype:KO | Treatment:72h 6C	Batch=B8; RAW_FILE_NAME(Raw_file_name)=Pos_13.raw
SUBJECT_SAMPLE_FACTORS           	45KO7	Pos_14	Sample source:Serum | Sample type:Serum | Genotype:KO | Treatment:72h 6C	Batch=B8; RAW_FILE_NAME(Raw_file_name)=Pos_14.raw
SUBJECT_SAMPLE_FACTORS           	LvControl1	C1	Sample source:Serum | Sample type:Serum | Genotype:Control | Treatment:72h 6C	Batch=B9; RAW_FILE_NAME(Raw_file_name)=C1.raw
SUBJECT_SAMPLE_FACTORS           	LvControl2	C2	Sample source:Serum | Sample type:Serum | Genotype:Control | Treatment:72h 6C	Batch=B9; RAW_FILE_NAME(Raw_file_name)=C2.raw
SUBJECT_SAMPLE_FACTORS           	LvControl3	C3	Sample source:Serum | Sample type:Serum | Genotype:Control | Treatment:72h 6C	Batch=B9; RAW_FILE_NAME(Raw_file_name)=C3.raw
SUBJECT_SAMPLE_FACTORS           	LvControl4	C4	Sample source:Serum | Sample type:Serum | Genotype:Control | Treatment:72h 6C	Batch=B9; RAW_FILE_NAME(Raw_file_name)=C4.raw
SUBJECT_SAMPLE_FACTORS           	LvControl5	C5	Sample source:Serum | Sample type:Serum | Genotype:Control | Treatment:72h 6C	Batch=B9; RAW_FILE_NAME(Raw_file_name)=C5.raw
SUBJECT_SAMPLE_FACTORS           	LvControl6	C6	Sample source:Serum | Sample type:Serum | Genotype:Control | Treatment:72h 6C	Batch=B9; RAW_FILE_NAME(Raw_file_name)=C6.raw
SUBJECT_SAMPLE_FACTORS           	Slc25a45AlbKO1	C7	Sample source:Serum | Sample type:Serum | Genotype:Slc25a45AlbKO | Treatment:72h 6C	Batch=B9; RAW_FILE_NAME(Raw_file_name)=C7.raw
SUBJECT_SAMPLE_FACTORS           	Slc25a45AlbKO2	C8	Sample source:Serum | Sample type:Serum | Genotype:Slc25a45AlbKO | Treatment:72h 6C	Batch=B9; RAW_FILE_NAME(Raw_file_name)=C8.raw
SUBJECT_SAMPLE_FACTORS           	Slc25a45AlbKO3	C9	Sample source:Serum | Sample type:Serum | Genotype:Slc25a45AlbKO | Treatment:72h 6C	Batch=B9; RAW_FILE_NAME(Raw_file_name)=C9.raw
SUBJECT_SAMPLE_FACTORS           	Slc25a45AlbKO4	C10	Sample source:Serum | Sample type:Serum | Genotype:Slc25a45AlbKO | Treatment:72h 6C	Batch=B9; RAW_FILE_NAME(Raw_file_name)=C10.raw
SUBJECT_SAMPLE_FACTORS           	Slc25a45AlbKO5	C11	Sample source:Serum | Sample type:Serum | Genotype:Slc25a45AlbKO | Treatment:72h 6C	Batch=B9; RAW_FILE_NAME(Raw_file_name)=C11.raw
SUBJECT_SAMPLE_FACTORS           	Slc25a45AlbKO6	C12	Sample source:Serum | Sample type:Serum | Genotype:Slc25a45AlbKO | Treatment:72h 6C	Batch=B9; RAW_FILE_NAME(Raw_file_name)=C12.raw
#COLLECTION
CO:COLLECTION_SUMMARY            	Serum was taken at termination of study. 10 uL was mixed with metabolomics
CO:COLLECTION_SUMMARY            	extraction solvent (40% MeOH, 40% acetonitrile). Cells were counted for whole
CO:COLLECTION_SUMMARY            	cell-tracing studies and normalized to protein prior to extraction with
CO:COLLECTION_SUMMARY            	metabolomics extraction solvent. Mitochondria were normalized to protein before
CO:COLLECTION_SUMMARY            	extraction with metabolomics extraction solvent
CO:SAMPLE_TYPE                   	Mouse blood serum, 293 cells, primary hepatocytes, e.coli, inguinal cell
CO:SAMPLE_TYPE                   	mitochondria and 293 cell mitochondria
CO:COLLECTION_METHOD             	10 uL of mouse serum mixed 90 uL extraction solvent - 23000g spin for 15 min;
CO:COLLECTION_METHOD             	Whole cells on ice with extraction solvent for 10 minutes - 23000g spin for 15
CO:COLLECTION_METHOD             	min; 100 ug of isolated mitochondria mixed with 80 uL of extraction solvent -
CO:COLLECTION_METHOD             	23000g spin for 15 min; For E. coli, twenty OD600*mL units of bacteria
CO:COLLECTION_METHOD             	transformed with empty vector or overexpressing MBP-hSLC25A45 were resuspended
CO:COLLECTION_METHOD             	in 1 mL of potassium HEPES buffer. For timecourse assays, 10 µM D9-TML was
CO:COLLECTION_METHOD             	added to the sample with shaking at room temperature and aliquots taken at 1, 5,
CO:COLLECTION_METHOD             	10, 20, and 60 minutes. Metabolites were extracted with ice-cold solvent
#TREATMENT
TR:TREATMENT_SUMMARY             	Human embryonic kidney 293 cells (45 KO, WT or 45 Rescue) were treated with 10
TR:TREATMENT_SUMMARY             	uM D9-TML for 4 h prior to metabolite extraction. Primary hepatocytes from
TR:TREATMENT_SUMMARY             	SLC25A45 KO mice or littermate controls were also used for D9-TML tracing for
TR:TREATMENT_SUMMARY             	various timepoints. Serum from SLC25A45 whole body KO, liver-specific KO
TR:TREATMENT_SUMMARY             	(Slc25a45AlbKO) or littermate controls was used at study termination. Select
TR:TREATMENT_SUMMARY             	mice were exposed to cold for 72h (at 6 degrees C) prior to termination.
TR:TREATMENT_SUMMARY             	Isolated mitochondria from inguinal adipocytes (empty vector or pMSCV-cre to
TR:TREATMENT_SUMMARY             	induce slc25a45 ko)or 293 cells were treated with 10 uM D9-TML for five minutes
TR:TREATMENT_SUMMARY             	at room temperature, then washed prior to extraction for metabolomics
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	For metabolomics, samples were extracted with 40% MeOH, 40% acetonitrile. For
SP:SAMPLEPREP_SUMMARY            	lipidomics, samples were extracted in butanol/methanol (1:1) with 5 mM ammonium
SP:SAMPLEPREP_SUMMARY            	formate.
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_SUMMARY        	Lipidomics
CH:CHROMATOGRAPHY_TYPE           	Reversed phase
CH:INSTRUMENT_NAME               	Thermo Vanquish
CH:COLUMN_NAME                   	Waters ACQUITY UPLC CSH C18 (100 x 2.1mm,1.7um)
CH:SOLVENT_A                     	Water:acetonitrile 40:60; 10 mM ammonium formate and 0.1% formic acid
CH:SOLVENT_B                     	Isopropranol:acetonitrile 90:10; 10 mM ammonium formate and 0.1% formic acid
CH:FLOW_GRADIENT                 	20% B from 0 to 3 min, 55% B at 7 min, 65% B at 15 min, 70% B at 21 min, 88% B
CH:FLOW_GRADIENT                 	at 23 min, 100% B at 24 min held until 26 min, and 20% B at 28 min and held
CH:FLOW_GRADIENT                 	until 30 min
CH:FLOW_RATE                     	0.35 mL/min
CH:COLUMN_TEMPERATURE            	4
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
#MS
MS:INSTRUMENT_NAME               	Thermo Orbitrap Exploris 480
MS:INSTRUMENT_TYPE               	Orbitrap
MS:MS_TYPE                       	ESI
MS:ION_MODE                      	POSITIVE
MS:MS_COMMENTS                   	The ion source was H-ESI. Spray voltage was 3500 V for positive ions, and 2500 V
MS:MS_COMMENTS                   	for negative ions. Sheath gas was set at 50 arbitrary unit (Arb), auxiliary gas
MS:MS_COMMENTS                   	15 Arb, sweep gas 1 Arb, ion transfer tube at 325 °C, and vaporizer 350 °C.
MS:MS_COMMENTS                   	The scan mode was data dependent (dd)-MS2, covering 150-1600 m/z in both
MS:MS_COMMENTS                   	positive and negative polarities. Precursor ion scan had resolution 60,000. For
MS:MS_COMMENTS                   	product ion scan, the resolution was 15,000, isolation width 1.0 m/z, and
MS:MS_COMMENTS                   	collision energy 25%. Thermo Scientific LipidSearch software version 5.0 was
MS:MS_COMMENTS                   	used for lipid identification and quantitation. First, the product search mode
MS:MS_COMMENTS                   	was used to identify lipids based on the exact mass of the precursor ions and
MS:MS_COMMENTS                   	the MS2 mass spectra of product ion scan. The precursor and product tolerance
MS:MS_COMMENTS                   	was 10 ppm. The absolute intensity threshold of precursor ions and the relative
MS:MS_COMMENTS                   	intensity threshold of product ions were set to 30000 and 1%, respectively.
MS:MS_COMMENTS                   	Next, the search results from all samples were aligned within a retention time
MS:MS_COMMENTS                   	tolerance of 0.25 min. The annotated lipids were then filtered to reduce false
MS:MS_COMMENTS                   	positives by only including the lipids with a total grade of A and B.
#MS_METABOLITE_DATA
MS_METABOLITE_DATA:UNITS	Peak area
MS_METABOLITE_DATA_START
Samples	Pos_1	Pos_2	Pos_3	Pos_4	Pos_5	Pos_6	Pos_7	Pos_8	Pos_9	Pos_10	Pos_11	Pos_12	Pos_13	Pos_14	C1	C2	C3	C4	C5	C6	C7	C8	C9	C10	C11	C12
Factors	Sample source:Serum | Sample type:Serum | Genotype:Control | Treatment:72h 6C	Sample source:Serum | Sample type:Serum | Genotype:Control | Treatment:72h 6C	Sample source:Serum | Sample type:Serum | Genotype:Control | Treatment:72h 6C	Sample source:Serum | Sample type:Serum | Genotype:Control | Treatment:72h 6C	Sample source:Serum | Sample type:Serum | Genotype:Control | Treatment:72h 6C	Sample source:Serum | Sample type:Serum | Genotype:Control | Treatment:72h 6C	Sample source:Serum | Sample type:Serum | Genotype:Control | Treatment:72h 6C	Sample source:Serum | Sample type:Serum | Genotype:KO | Treatment:72h 6C	Sample source:Serum | Sample type:Serum | Genotype:KO | Treatment:72h 6C	Sample source:Serum | Sample type:Serum | Genotype:KO | Treatment:72h 6C	Sample source:Serum | Sample type:Serum | Genotype:KO | Treatment:72h 6C	Sample source:Serum | Sample type:Serum | Genotype:KO | Treatment:72h 6C	Sample source:Serum | Sample type:Serum | Genotype:KO | Treatment:72h 6C	Sample source:Serum | Sample type:Serum | Genotype:KO | Treatment:72h 6C	Sample source:Serum | Sample type:Serum | Genotype:Control | Treatment:72h 6C	Sample source:Serum | Sample type:Serum | Genotype:Control | Treatment:72h 6C	Sample source:Serum | Sample type:Serum | Genotype:Control | Treatment:72h 6C	Sample source:Serum | Sample type:Serum | Genotype:Control | Treatment:72h 6C	Sample source:Serum | Sample type:Serum | Genotype:Control | Treatment:72h 6C	Sample source:Serum | Sample type:Serum | Genotype:Control | Treatment:72h 6C	Sample source:Serum | Sample type:Serum | Genotype:Slc25a45AlbKO | Treatment:72h 6C	Sample source:Serum | Sample type:Serum | Genotype:Slc25a45AlbKO | Treatment:72h 6C	Sample source:Serum | Sample type:Serum | Genotype:Slc25a45AlbKO | Treatment:72h 6C	Sample source:Serum | Sample type:Serum | Genotype:Slc25a45AlbKO | Treatment:72h 6C	Sample source:Serum | Sample type:Serum | Genotype:Slc25a45AlbKO | Treatment:72h 6C	Sample source:Serum | Sample type:Serum | Genotype:Slc25a45AlbKO | Treatment:72h 6C
AcCa12:1)	36001	87265	65176	35432	55627	46866	46265	24732	29475	31712	37147	25458	31310	26479	0	0	0	0	0	0	0	0	0	0	0	0
AcCa(14:0)	132100	290430	211360	282360	282570	243020	168300	63162	108180	169390	128740	66279	131940	79556	185970	371390	237440	154470	64764	229280	570900	134520	90884	350810	338820	181340
AcCa(14:1)	55666	376330	129410	181080	292290	185850	196290	58365	142770	134760	47931	78781	105540	107760	233910	182820	324880	179440	80614	284490	318440	173900	85726	380440	335650	237570
AcCa(14:2)	40027	62602	70639	74750	66479	45256	48131	22981	35400	46416	44085	23690	42684	43890	16941	49270	66830	8645.5	6446.6	42809	88166	32130	0	51296	42181	39533
AcCa(16:2)	26175	65851	53658	66147	64849	52828	39214	13934	18789	43484	28091	13826	29956	20748	39466	46451	54657	23049	5531.3	35630	120900	20775	11992	77957	57705	28697
AcCa(18:0)	130310	122110	134410	117710	118070	108540	100590	103030	99499	129320	90622	85061	123260	76371	251720	278870	289710	169560	183000	244160	331200	219750	171000	213140	247590	197540
AcCa(18:2)	83222	238210	232980	186250	123210	180020	150390	60601	123620	202540	132820	82517	156090	89639	0	0	0	0	0	0	0	0	0	0	0	0
DAP(O-14:0)	250130	268630	243910	279420	252530	261250	277830	248270	252280	240520	258800	243520	210680	211010	0	0	0	0	0	0	0	0	0	0	0	0
PI(50:3)	444240	479950	395330	475570	466170	452590	464140	350550	434140	384730	427380	439910	402730	433320	0	0	0	0	0	0	0	0	0	0	0	0
SPH(d16:0)	6860900	7014000	4404000	3074820	5863000	4734200	1905140	4453500	2089330	2008780	3055900	2657670	1801120	2404580	0	0	0	0	0	0	0	0	0	0	0	0
SPH(d16:1)	1067700	67092	516170	877920	974870	784470	473340	524790	405480	481510	366740	383970	440930	312770	0	0	0	0	0	0	0	0	0	0	0	0
SPH(d16:2)	223360	546980	236940	273570	644410	456260	153820	228290	130360	71666	187240	143640	180880	175110	0	0	0	0	0	0	0	0	0	0	0	0
LPC(17:1)	67570	45871	80505	45933	51224	35345	50741	98133	92064	64482	60047	76898	71990	54871	0	0	0	0	0	0	0	0	0	0	0	0
LPC(18:1)	3799800	3142400	3090620	4104800	4079190	2510060	5534000	10021200	7500400	6993700	4391100	2829860	6777900	4642000	41944600	43719000	49391000	28085400	27652000	37738400	39503000	37000100	28701700	42928000	35972100	33094100
LPC(20:0)	487690	158840	280350	132210	205570	119250	281980	560440	370290	326640	296670	449380	367660	263310	187990	233610	176170	177460	305940	142650	58232	271500	250590	100150	152080	206550
MePC(30:1)	1371370	960290	1249310	937070	1075990	1154930	1385410	1377630	1395390	1324980	1442850	1285040	1421750	1328520	0	0	0	0	0	0	0	0	0	0	0	0
MePC(41:6)	159470	51028	144120	59433	83658	60966	124340	159610	152700	141140	122260	158380	154630	109500	841730	922200	765210	736200	888510	661830	562670	752560	715330	500120	630800	710230
PC(40:5)	114740	29366	124170	89469	93894	56235	95107	213940	177590	194340	196330	110660	119800	109680	0	0	0	0	0	0	0	0	0	0	0	0
PC(42:7)	251380	100320	226540	124220	143610	117000	134690	227170	205930	233400	212520	216070	194570	171670	0	0	0	0	0	0	0	0	0	0	0	0
PC(31:0)	171500	120390	126720	122260	93455	71180	111550	259690	230700	223120	82529	101570	181990	169500	1612200	2151900	2347300	1280700	1056800	1954900	1624200	838220	449610	462120	998470	1500000
PC(38:2)	1807200	414480	865110	523250	674350	467070	768840	2242300	1630800	1190300	983790	1032400	1519600	832130	858410	961690	1083500	711700	798820	761360	705600	791320	830910	737390	565970	761160
PC(O-39:8)	366760	289940	373740	317510	278880	296020	311980	375420	395020	375290	317900	338600	418770	361100	0	0	0	0	0	0	0	0	0	0	0	0
PI(38:7)	130640	56067	132900	90419	89102	62268	91289	179290	154870	136380	104680	115120	129800	103170	1344000	1457300	1539300	1123100	1392100	1066200	1071000	1590500	1334100	1236100	1091000	1404600
SM(d39:1)	151430	96772	148750	110020	105190	88650	127570	170930	158250	178150	115790	154870	162930	105040	0	0	0	0	0	0	0	0	0	0	0	0
AEA(12:0)	0	0	0	0	0	0	0	0	0	0	0	0	0	0	10858	42447	28480	80370	31152	37218	21572	98153	43639	46634	132300	3192
AcCa(12:0)	0	0	0	0	0	0	0	0	0	0	0	0	0	0	88619	100900	104730	52440	14496	109100	211700	40943	11481	130250	110630	74256
AcCa(20:4)	0	0	0	0	0	0	0	0	0	0	0	0	0	0	78462	97674	87691	46700	27476	80818	161600	41309	27188	94924	91004	68829
AcCa(20:5)	0	0	0	0	0	0	0	0	0	0	0	0	0	0	46960	61908	39616	19002	3744	36735	64989	36596	24193	52166	42815	36232
AcCa(2:0)	0	0	0	0	0	0	0	0	0	0	0	0	0	0	13228000	16642000	13001000	14354000	12828000	13690000	16600000	12472000	6863100	12905000	15994000	16169000
AcCa(3:0)	0	0	0	0	0	0	0	0	0	0	0	0	0	0	373950	569740	392750	329690	530900	376450	563570	423240	250620	923070	544560	380320
AcCa(18:1)	0	0	0	0	0	0	0	0	0	0	0	0	0	0	714470	883720	777460	598470	203440	638110	1397000	415510	283110	830160	829120	536820
MS_METABOLITE_DATA_END
#METABOLITES
METABOLITES_START
metabolite_name
AcCa12:1)
AcCa(14:0)
AcCa(14:1)
AcCa(14:2)
AcCa(16:2)
AcCa(18:0)
AcCa(18:2)
DAP(O-14:0)
PI(50:3)
SPH(d16:0)
SPH(d16:1)
SPH(d16:2)
LPC(17:1)
LPC(18:1)
LPC(20:0)
MePC(30:1)
MePC(41:6)
PC(40:5)
PC(42:7)
PC(31:0)
PC(38:2)
PC(O-39:8)
PI(38:7)
SM(d39:1)
AEA(12:0)
AcCa(12:0)
AcCa(20:4)
AcCa(20:5)
AcCa(2:0)
AcCa(3:0)
AcCa(18:1)
METABOLITES_END
#END