#METABOLOMICS WORKBENCH FeiTeng2025_20250516_232907 DATATRACK_ID:5920 STUDY_ID:ST003921 ANALYSIS_ID:AN006438 PROJECT_ID:PR002454 VERSION 1 CREATED_ON May 19, 2025, 9:44 am #PROJECT PR:PROJECT_TITLE Metabolic fingerprinting enables rapid, label-free histopathology in gastric PR:PROJECT_TITLE cancer diagnosis and prognostic prediction PR:PROJECT_SUMMARY Nanoparticle-enhanced-LDI-MS-based metabolic profiling of PR:PROJECT_SUMMARY formalin-fixed-paraffin-embedded tissues identifies diagnostic fingerprints for PR:PROJECT_SUMMARY gastric cancer, correlating with pentose phosphate pathway dysregulation PR:INSTITUTE Fudan University PR:DEPARTMENT Minhang Hospital PR:LAST_NAME Teng PR:FIRST_NAME Fei PR:ADDRESS 170 Xinsong Road PR:EMAIL prof_tengfei@fudan.edu.cn PR:PHONE 18817618785 #STUDY ST:STUDY_TITLE Metabolic fingerprinting enables rapid, label-free histopathology in gastric ST:STUDY_TITLE cancer diagnosis and prognostic prediction ST:STUDY_SUMMARY Histopathological evaluation is a cornerstone of cancer identification but often ST:STUDY_SUMMARY involves time-consuming labeling processes (~days per sample) and ST:STUDY_SUMMARY experience-dependent interpretation. Herein, we introduce a rapid (~40 minutes ST:STUDY_SUMMARY per sample) label-free method based on nanoparticle-enhanced LDI metabolic ST:STUDY_SUMMARY fingerprinting for gastric cancer (GC) assessment. Untargeted metabolomics of ST:STUDY_SUMMARY 284 paired tissues distinguishes malignant from benign tissues (AUC of 0.979), ST:STUDY_SUMMARY identifies tumor subtypes (AUC of 0.963), and assesses prognosis (p < 0.05) ST:STUDY_SUMMARY without specialized pathologists. This approach marks a substantial advancement ST:STUDY_SUMMARY in histopathological evaluation and shows great potential for large-scale ST:STUDY_SUMMARY clinical applications. ST:INSTITUTE Fudan University ST:DEPARTMENT Minhang Hospital ST:LAST_NAME Teng ST:FIRST_NAME Fei ST:ADDRESS 170 Xinsong Road ST:EMAIL prof_tengfei@fudan.edu.cn ST:PHONE 18817618785 #SUBJECT SU:SUBJECT_TYPE Human SU:SUBJECT_SPECIES Homo sapiens SU:TAXONOMY_ID 9606 SU:GENDER Male and female #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data SUBJECT_SAMPLE_FACTORS - ANT_1 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_2 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_3 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_4 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_5 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_6 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_7 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_8 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_9 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_10 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_11 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_12 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_13 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_14 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_15 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_16 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_17 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_18 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_19 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_20 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_21 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_22 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_23 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_24 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_25 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_26 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_27 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_28 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_29 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_30 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_31 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_32 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_33 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_34 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_35 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_36 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_37 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_38 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_39 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_40 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_41 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_42 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_43 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_44 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_45 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_46 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_47 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_48 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_49 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_50 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_51 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_52 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_53 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_54 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_55 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_56 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_57 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_58 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_59 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_60 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_61 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_62 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_63 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_64 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_65 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_66 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_67 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_68 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_69 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_70 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_71 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_72 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_73 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_74 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_75 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_76 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_77 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_78 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_79 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_80 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_81 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_82 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_83 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_84 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_85 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_86 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_87 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_88 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_89 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_90 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_91 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_92 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_93 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_94 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_95 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_96 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_97 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_98 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_99 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_100 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_101 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_102 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_103 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_104 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_105 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_106 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_107 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_108 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_109 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_110 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_111 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_112 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_113 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_114 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_115 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_116 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_117 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_118 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_119 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_120 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_121 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_122 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_123 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_124 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_125 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_126 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_127 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_128 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_129 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_130 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_131 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_132 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_133 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_134 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_135 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_136 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_137 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_138 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_139 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_140 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_141 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_142 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_1 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_2 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_3 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_4 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_5 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_6 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_7 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_8 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_9 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_10 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_11 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_12 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_13 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_14 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_15 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_16 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_17 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_18 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_19 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_20 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_21 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_22 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_23 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_24 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_25 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_26 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_27 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_28 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_29 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_30 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_31 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_32 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_33 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_34 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_35 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_36 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_37 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_38 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_39 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_40 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_41 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_42 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_43 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_44 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_45 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_46 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_47 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_48 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_49 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_50 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_51 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_52 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_53 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_54 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_55 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_56 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_57 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_58 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_59 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_60 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_61 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_62 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_63 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_64 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_65 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_66 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_67 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_68 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_69 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_70 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_71 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_72 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_73 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_74 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_75 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_76 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_77 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_78 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_79 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_80 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_81 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_82 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_83 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_84 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_85 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_86 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_87 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_88 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_89 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_90 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_91 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_92 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_93 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_94 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_95 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_96 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_97 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_98 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_99 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_100 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_101 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_102 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_103 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_104 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_105 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_106 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_107 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_108 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_109 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_110 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_111 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_112 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_113 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_114 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_115 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_116 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_117 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_118 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_119 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_120 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_121 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_122 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_123 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_124 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_125 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_126 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_127 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_128 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_129 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_130 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_131 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_132 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_133 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_134 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_135 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_136 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_137 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_138 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_139 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_140 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_141 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_142 Diagnosis:Malignant | Sample source:FFPE tissue #COLLECTION CO:COLLECTION_SUMMARY Formalin-fixed paraffin-embedded (FFPE) gastric cancer tissues and paired CO:COLLECTION_SUMMARY adjacent normal (benign) tissues (>2 cm from tumor margins) were collected under CO:COLLECTION_SUMMARY standardized surgical protocols. Tissue cores (1 mm diameter, 20 μm thickness) CO:COLLECTION_SUMMARY were microdissected using a tissue microarray platform, validated by CO:COLLECTION_SUMMARY histopathological review to ensure purity. CO:SAMPLE_TYPE gastric cancer tissue #TREATMENT TR:TREATMENT_SUMMARY None #SAMPLEPREP SP:SAMPLEPREP_SUMMARY （1）Tissue samples were dissolved in 80% methanol to release metabolites. (2) SP:SAMPLEPREP_SUMMARY Samples were heated at 70°C for 20 minutes to remove paraffin wax, a step that SP:SAMPLEPREP_SUMMARY can be performed in batches to enhance throughput. (3) Samples were placed in an SP:SAMPLEPREP_SUMMARY ice bath for 10 minutes to solidify the wax. (4) Centrifugation was performed at SP:SAMPLEPREP_SUMMARY 10,000 rpm for 10 minutes at 4°C to separate the wax. (5) The supernatant SP:SAMPLEPREP_SUMMARY containing the extracted metabolites was carefully collected. (6) The SP:SAMPLEPREP_SUMMARY supernatant was mixed with a matrix and spotted onto an LDI plate. (7) The plate SP:SAMPLEPREP_SUMMARY was inserted into the mass spectrometer for metabolic fingerprint acquisition. #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE None (Direct infusion) CH:INSTRUMENT_NAME none CH:COLUMN_NAME none CH:SOLVENT_A none CH:SOLVENT_B none CH:FLOW_GRADIENT none CH:FLOW_RATE none CH:COLUMN_TEMPERATURE none #ANALYSIS AN:ANALYSIS_TYPE MS #MS MS:INSTRUMENT_NAME Bruker Autoflex speed TOF/TOF MS:INSTRUMENT_TYPE TOF MS:MS_TYPE MALDI MS:ION_MODE POSITIVE MS:MS_COMMENTS Metabolites extracted from FFPE tissue samples were directly detected without MS:MS_COMMENTS the need for further sample pretreatment, following a standardized profiling MS:MS_COMMENTS workflow. In a typical metabolic analysis process based on NPELDI MS, 1 µL of MS:MS_COMMENTS the FFPE tissue metabolite solution was deposited onto the microarray chip. MS:MS_COMMENTS Subsequently, ferric nanoparticle solution (1 µL) with a concentration of 1 mg MS:MS_COMMENTS mL-1 was distributed onto the dried FFPE tissue metabolites. Mass spectra of MS:MS_COMMENTS metabolites were recorded using a Bruker Autoflex MALDI TOF/TOF mass MS:MS_COMMENTS spectrometer (Bruker Daltonics, Bremen, Germany), with Nd:YAG lasers of 355 nm. MS:MS_COMMENTS During detection, the pulse laser was optimized at a frequency of 1000 Hz (2000 MS:MS_COMMENTS shots), a delay time of 200 ns, and an acceleration voltage of 20 kV. Biomarker MS:MS_COMMENTS identification was performed on Solarix 7.0T (Fourier Transform Ion Cyclotron MS:MS_COMMENTS Resonance Mass Spectrometry, FT-ICR-MS). The related molecular formulas were MS:MS_COMMENTS confirmed in the Bruker Compass Data Analysis software (version 5.0) based on MS:MS_COMMENTS accurate mass (< 10 ppm). The Human Metabolome Database (HMDB) was utilized for MS:MS_COMMENTS searching candidate metabolites. All MALDI-MS data were acquired using the MS:MS_COMMENTS reflectron mode. MS:MS_RESULTS_FILE ST003921_AN006438_Results.txt UNITS:Peak intensity Has m/z:Yes Has RT:No RT units:No RT data #END #METABOLOMICS WORKBENCH FeiTeng2025_20250516_232907 DATATRACK_ID:5920 STUDY_ID:ST003921 ANALYSIS_ID:AN006438 PROJECT_ID:PR002454 VERSION 1 CREATED_ON May 19, 2025, 9:44 am #PROJECT PR:PROJECT_TITLE Metabolic fingerprinting enables rapid, label-free histopathology in gastric PR:PROJECT_TITLE cancer diagnosis and prognostic prediction PR:PROJECT_SUMMARY Nanoparticle-enhanced-LDI-MS-based metabolic profiling of PR:PROJECT_SUMMARY formalin-fixed-paraffin-embedded tissues identifies diagnostic fingerprints for PR:PROJECT_SUMMARY gastric cancer, correlating with pentose phosphate pathway dysregulation PR:INSTITUTE Fudan University PR:DEPARTMENT Minhang Hospital PR:LAST_NAME Teng PR:FIRST_NAME Fei PR:ADDRESS 170 Xinsong Road PR:EMAIL prof_tengfei@fudan.edu.cn PR:PHONE 18817618785 #STUDY ST:STUDY_TITLE Metabolic fingerprinting enables rapid, label-free histopathology in gastric ST:STUDY_TITLE cancer diagnosis and prognostic prediction ST:STUDY_SUMMARY Histopathological evaluation is a cornerstone of cancer identification but often ST:STUDY_SUMMARY involves time-consuming labeling processes (~days per sample) and ST:STUDY_SUMMARY experience-dependent interpretation. Herein, we introduce a rapid (~40 minutes ST:STUDY_SUMMARY per sample) label-free method based on nanoparticle-enhanced LDI metabolic ST:STUDY_SUMMARY fingerprinting for gastric cancer (GC) assessment. Untargeted metabolomics of ST:STUDY_SUMMARY 284 paired tissues distinguishes malignant from benign tissues (AUC of 0.979), ST:STUDY_SUMMARY identifies tumor subtypes (AUC of 0.963), and assesses prognosis (p < 0.05) ST:STUDY_SUMMARY without specialized pathologists. This approach marks a substantial advancement ST:STUDY_SUMMARY in histopathological evaluation and shows great potential for large-scale ST:STUDY_SUMMARY clinical applications. ST:INSTITUTE Fudan University ST:DEPARTMENT Minhang Hospital ST:LAST_NAME Teng ST:FIRST_NAME Fei ST:ADDRESS 170 Xinsong Road ST:EMAIL prof_tengfei@fudan.edu.cn ST:PHONE 18817618785 #SUBJECT SU:SUBJECT_TYPE Human SU:SUBJECT_SPECIES Homo sapiens SU:TAXONOMY_ID 9606 SU:GENDER Male and female #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data SUBJECT_SAMPLE_FACTORS - ANT_1 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_2 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_3 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_4 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_5 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_6 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_7 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_8 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_9 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_10 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_11 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_12 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_13 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_14 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_15 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_16 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_17 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_18 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_19 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_20 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_21 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_22 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_23 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_24 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_25 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_26 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_27 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_28 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_29 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_30 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_31 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_32 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_33 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_34 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_35 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_36 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_37 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_38 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_39 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_40 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_41 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_42 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_43 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_44 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_45 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_46 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_47 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_48 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_49 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_50 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_51 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_52 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_53 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_54 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_55 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_56 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_57 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_58 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_59 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_60 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_61 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_62 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_63 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_64 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_65 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_66 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_67 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_68 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_69 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_70 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_71 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_72 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_73 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_74 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_75 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_76 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_77 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_78 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_79 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_80 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_81 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_82 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_83 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_84 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_85 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_86 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_87 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_88 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_89 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_90 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_91 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_92 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_93 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_94 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_95 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_96 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_97 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_98 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_99 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_100 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_101 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_102 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_103 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_104 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_105 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_106 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_107 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_108 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_109 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_110 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_111 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_112 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_113 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_114 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_115 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_116 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_117 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_118 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_119 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_120 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_121 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_122 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_123 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_124 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_125 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_126 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_127 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_128 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_129 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_130 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_131 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_132 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_133 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_134 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_135 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_136 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_137 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_138 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_139 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_140 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_141 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - ANT_142 Diagnosis:Benign | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_1 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_2 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_3 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_4 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_5 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_6 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_7 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_8 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_9 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_10 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_11 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_12 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_13 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_14 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_15 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_16 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_17 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_18 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_19 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_20 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_21 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_22 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_23 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_24 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_25 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_26 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_27 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_28 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_29 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_30 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_31 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_32 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_33 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_34 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_35 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_36 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_37 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_38 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_39 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_40 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_41 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_42 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_43 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_44 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_45 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_46 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_47 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_48 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_49 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_50 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_51 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_52 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_53 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_54 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_55 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_56 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_57 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_58 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_59 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_60 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_61 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_62 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_63 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_64 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_65 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_66 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_67 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_68 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_69 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_70 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_71 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_72 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_73 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_74 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_75 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_76 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_77 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_78 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_79 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_80 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_81 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_82 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_83 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_84 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_85 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_86 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_87 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_88 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_89 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_90 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_91 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_92 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_93 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_94 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_95 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_96 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_97 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_98 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_99 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_100 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_101 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_102 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_103 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_104 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_105 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_106 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_107 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_108 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_109 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_110 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_111 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_112 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_113 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_114 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_115 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_116 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_117 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_118 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_119 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_120 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_121 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_122 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_123 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_124 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_125 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_126 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_127 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_128 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_129 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_130 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_131 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_132 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_133 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_134 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_135 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_136 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_137 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_138 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_139 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_140 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_141 Diagnosis:Malignant | Sample source:FFPE tissue SUBJECT_SAMPLE_FACTORS - GCT_142 Diagnosis:Malignant | Sample source:FFPE tissue #COLLECTION CO:COLLECTION_SUMMARY Formalin-fixed paraffin-embedded (FFPE) gastric cancer tissues and paired CO:COLLECTION_SUMMARY adjacent normal (benign) tissues (>2 cm from tumor margins) were collected under CO:COLLECTION_SUMMARY standardized surgical protocols. Tissue cores (1 mm diameter, 20 μm thickness) CO:COLLECTION_SUMMARY were microdissected using a tissue microarray platform, validated by CO:COLLECTION_SUMMARY histopathological review to ensure purity. CO:SAMPLE_TYPE gastric cancer tissue #TREATMENT TR:TREATMENT_SUMMARY None #SAMPLEPREP SP:SAMPLEPREP_SUMMARY （1）Tissue samples were dissolved in 80% methanol to release metabolites. (2) SP:SAMPLEPREP_SUMMARY Samples were heated at 70°C for 20 minutes to remove paraffin wax, a step that SP:SAMPLEPREP_SUMMARY can be performed in batches to enhance throughput. (3) Samples were placed in an SP:SAMPLEPREP_SUMMARY ice bath for 10 minutes to solidify the wax. (4) Centrifugation was performed at SP:SAMPLEPREP_SUMMARY 10,000 rpm for 10 minutes at 4°C to separate the wax. (5) The supernatant SP:SAMPLEPREP_SUMMARY containing the extracted metabolites was carefully collected. (6) The SP:SAMPLEPREP_SUMMARY supernatant was mixed with a matrix and spotted onto an LDI plate. (7) The plate SP:SAMPLEPREP_SUMMARY was inserted into the mass spectrometer for metabolic fingerprint acquisition. #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE None (Direct infusion) CH:INSTRUMENT_NAME none CH:COLUMN_NAME none CH:SOLVENT_A none CH:SOLVENT_B none CH:FLOW_GRADIENT none CH:FLOW_RATE none CH:COLUMN_TEMPERATURE none #ANALYSIS AN:ANALYSIS_TYPE MS #MS MS:INSTRUMENT_NAME Bruker Autoflex speed TOF/TOF MS:INSTRUMENT_TYPE TOF MS:MS_TYPE MALDI MS:ION_MODE POSITIVE MS:MS_COMMENTS Metabolites extracted from FFPE tissue samples were directly detected without MS:MS_COMMENTS the need for further sample pretreatment, following a standardized profiling MS:MS_COMMENTS workflow. In a typical metabolic analysis process based on NPELDI MS, 1 µL of MS:MS_COMMENTS the FFPE tissue metabolite solution was deposited onto the microarray chip. MS:MS_COMMENTS Subsequently, ferric nanoparticle solution (1 µL) with a concentration of 1 mg MS:MS_COMMENTS mL-1 was distributed onto the dried FFPE tissue metabolites. Mass spectra of MS:MS_COMMENTS metabolites were recorded using a Bruker Autoflex MALDI TOF/TOF mass MS:MS_COMMENTS spectrometer (Bruker Daltonics, Bremen, Germany), with Nd:YAG lasers of 355 nm. MS:MS_COMMENTS During detection, the pulse laser was optimized at a frequency of 1000 Hz (2000 MS:MS_COMMENTS shots), a delay time of 200 ns, and an acceleration voltage of 20 kV. Biomarker MS:MS_COMMENTS identification was performed on Solarix 7.0T (Fourier Transform Ion Cyclotron MS:MS_COMMENTS Resonance Mass Spectrometry, FT-ICR-MS). The related molecular formulas were MS:MS_COMMENTS confirmed in the Bruker Compass Data Analysis software (version 5.0) based on MS:MS_COMMENTS accurate mass (< 10 ppm). The Human Metabolome Database (HMDB) was utilized for MS:MS_COMMENTS searching candidate metabolites. All MALDI-MS data were acquired using the MS:MS_COMMENTS reflectron mode. MS:MS_RESULTS_FILE ST003921_AN006438_Results.txt UNITS:Peak intensity Has m/z:Yes Has RT:No RT units:No RT data #END