#METABOLOMICS WORKBENCH Yuanye_Chi_20250513_192220 DATATRACK_ID:5912 STUDY_ID:ST003952 ANALYSIS_ID:AN006496 PROJECT_ID:PR001844
VERSION             	1
CREATED_ON             	June 4, 2025, 10:47 am
#PROJECT
PR:PROJECT_TITLE                 	Molecular Phenotypes of Null Alleles in Cells
PR:PROJECT_SUMMARY               	The 2020 NHGRI Strategic Vision laid out a set of “bold predictions for human
PR:PROJECT_SUMMARY               	genomics by 2030” including elucidating the biological function(s) of each
PR:PROJECT_SUMMARY               	human gene. The Molecular Phenotypes of Null Alleles in Cells (MorPhiC) seeks to
PR:PROJECT_SUMMARY               	address this element of the strategic vision. Through the comprehensive
PR:PROJECT_SUMMARY               	generation of null alleles for every human gene and then cataloging the
PR:PROJECT_SUMMARY               	resulting molecular and cellular phenotypes, the mechanisms that relate gene
PR:PROJECT_SUMMARY               	function to observed phenotypes can be determined. Furthermore, the resulting
PR:PROJECT_SUMMARY               	catalog of knockouts and phenotypes will be made available for broader use by
PR:PROJECT_SUMMARY               	the biomedical community. Although multiple approaches can be leveraged to
PR:PROJECT_SUMMARY               	measure molecular and cellular phenotypes resulting from gene knockouts,
PR:PROJECT_SUMMARY               	metabolomics and lipidomics (i.e., biochemical phenotyping) provides an avenue
PR:PROJECT_SUMMARY               	to understand the link between gene function and phenotypes at a molecular
PR:PROJECT_SUMMARY               	level. This project consists of studies performed to biochemical phenotype of
PR:PROJECT_SUMMARY               	cell lines and other samples generated as part of MorPhiC. Resources: 1.
PR:PROJECT_SUMMARY               	https://www.nih.gov/news-events/news-releases/nih-initiative-systematically-investigate-establish-function-every-human-gene
PR:PROJECT_SUMMARY               	2.
PR:PROJECT_SUMMARY               	https://www.genome.gov/research-funding/Funded-Programs-Projects/Molecular-Phenotypes-of-Null-Alleles-in-Cells
PR:INSTITUTE                     	The Jackson Laboratory for Genomic Medicine
PR:LABORATORY                    	Shuzhao Li Lab
PR:LAST_NAME                     	Chi
PR:FIRST_NAME                    	Yuanye
PR:ADDRESS                       	10 Discovery Dr, Farmington, CT
PR:EMAIL                         	yuanye.chi@jax.org
PR:PHONE                         	3395456866
#STUDY
ST:STUDY_TITLE                   	Comprehensive Untargeted LC-MS Metabolomics Analysis of STS/HSD3B1 Knockout
ST:STUDY_TITLE                   	iPSCs (supernatant)
ST:STUDY_SUMMARY                 	Human induced pluripotent stem cells (hIPSCs) are a key tool for biomedical
ST:STUDY_SUMMARY                 	research. Using CRISPR-cas9, null mutations were generated in KOLF2.2J hIPSCs
ST:STUDY_SUMMARY                 	and subsequently differentiated into primitive syncytium. In this study, null
ST:STUDY_SUMMARY                 	allele mutations were generated in STS and HSD3B1, genes encoding key enzymes in
ST:STUDY_SUMMARY                 	the steroidogenesis pathway. Cells were cultured on media with and without
ST:STUDY_SUMMARY                 	DHEA-S, a stimulator of steroidogenesis. Informed from previous studies (see
ST:STUDY_SUMMARY                 	PR001844), dansyl hydrazine and dansyl chloride (DnHz and DnCl respectively)
ST:STUDY_SUMMARY                 	were employed via an in-house method to enhance detection of specific sub
ST:STUDY_SUMMARY                 	metabolomes (e.g., estrogens and other steroids) in supernatant derived from the
ST:STUDY_SUMMARY                 	described cells. Derivatized samples were analyzed using a Thermo Scientific Q
ST:STUDY_SUMMARY                 	Exactive HF-X Mass Spectrometer coupled to a Thermo Vanquish Duo UHPLC Systems,
ST:STUDY_SUMMARY                 	equipped with an HES-II ionization source. The analysis uses a RP positive
ST:STUDY_SUMMARY                 	method optimized for derivatized samples. By comparing the steroid profiles of
ST:STUDY_SUMMARY                 	these samples, phenotypic differences can be explained at a molecular level.
ST:STUDY_SUMMARY                 	This is particularly useful for non-transcriptional factors that can otherwise
ST:STUDY_SUMMARY                 	be difficult to characterize with transcriptomics approaches. This study was
ST:STUDY_SUMMARY                 	funded, in part, through UM1HG012651 which established the JAX MorPhiC Center, a
ST:STUDY_SUMMARY                 	MorPhiC Phase 1 Data Production Research and Development Center at the Jackson
ST:STUDY_SUMMARY                 	Laboratory for Genomic Medicine. This study was funded, in part, through
ST:STUDY_SUMMARY                 	UM1HG012651 which established the JAX MorPhiC Center, a MorPhiC Phase 1 Data
ST:STUDY_SUMMARY                 	Production Research and Development Center at the Jackson Laboratory for Genomic
ST:STUDY_SUMMARY                 	Medicine.
ST:INSTITUTE                     	The Jackson Laboratory for Genomic Medicine
ST:LAST_NAME                     	Chi
ST:FIRST_NAME                    	Yuanye
ST:ADDRESS                       	10 Discovery Dr, Farmington, CT
ST:EMAIL                         	yuanye.chi@jax.org
ST:PHONE                         	3395456866
#SUBJECT
SU:SUBJECT_TYPE                  	Cultured cells
SU:SUBJECT_SPECIES               	Homo sapiens
SU:TAXONOMY_ID                   	9606
#FACTORS
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data
SUBJECT_SAMPLE_FACTORS           	-	Solvent_Blank_1_SZ_12042024_01	Sample source:Solvent_Blank | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=Solvent_Blank_1_SZ_12042024_01.mzML
SUBJECT_SAMPLE_FACTORS           	-	Process_Blank_12C_DnCl_1_SZ_12042024_02	Sample source:Process_Blank | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=Process_Blank_12C_DnCl_1_SZ_12042024_02.mzML
SUBJECT_SAMPLE_FACTORS           	-	Process_Blank_12C13C_DnCl_SZ_12042024_03	Sample source:Process_Blank | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=Process_Blank_12C13C_DnCl_SZ_12042024_03.mzML
SUBJECT_SAMPLE_FACTORS           	-	Pool_no_DnCl_1_12042024_04	Sample source:Pooled_Underivatized | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=Pool_no_DnCl_1_12042024_04.mzML
SUBJECT_SAMPLE_FACTORS           	-	Standard_12C_DnCl_1_SZ_12042024_05	Sample source:Standard_12C | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=Standard_12C_DnCl_1_SZ_12042024_05.mzML
SUBJECT_SAMPLE_FACTORS           	-	Pool_12C_DnCl_1_SZ_12042024_06	Sample source:Pool_12C | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=Pool_12C_DnCl_1_SZ_12042024_06.mzML
SUBJECT_SAMPLE_FACTORS           	-	Pool_12C13C_DnCl_1_SZ_12042024_07	Sample source:Pool_12C13C | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=Pool_12C13C_DnCl_1_SZ_12042024_07.mzML
SUBJECT_SAMPLE_FACTORS           	-	Qstd_1_SZ_12042024_08	Sample source:Qstd | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=Qstd_1_SZ_12042024_08.mzML
SUBJECT_SAMPLE_FACTORS           	-	WT_Basal_1_12C_DnCl_SZ_12042024_09	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=WT_Basal_1_12C_DnCl_SZ_12042024_09.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_KO_A02_DHEAS_R2_12C_DnCl_SZ_12042024_10	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_KO_A02_DHEAS_R2_12C_DnCl_SZ_12042024_10.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_KO_B03_DHEAS_R1_12C_DnCl_SZ_12042024_11	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_KO_B03_DHEAS_R1_12C_DnCl_SZ_12042024_11.mzML
SUBJECT_SAMPLE_FACTORS           	-	HSD3B1_PTC_D03_Basal_R1_12C_DnCl_SZ_12042024_12	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=HSD3B1_PTC_D03_Basal_R1_12C_DnCl_SZ_12042024_12.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_KO_A02_Basal_R1_12C_DnCl_SZ_12042024_13	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_KO_A02_Basal_R1_12C_DnCl_SZ_12042024_13.mzML
SUBJECT_SAMPLE_FACTORS           	-	WT_DHEAS_1_12C_DnCl_SZ_12042024_14	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=WT_DHEAS_1_12C_DnCl_SZ_12042024_14.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_KO_A01_Basal_R1_12C_DnCl_SZ_12042024_15	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_KO_A01_Basal_R1_12C_DnCl_SZ_12042024_15.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_KO_A01_DHEAS_R2_12C_DnCl_SZ_12042024_16	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_KO_A01_DHEAS_R2_12C_DnCl_SZ_12042024_16.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_CE_A06_Basal_R1_12C_DnCl_SZ_12042024_17	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_CE_A06_Basal_R1_12C_DnCl_SZ_12042024_17.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_PTC_C12_Basal_R2_12C_DnCl_SZ_12042024_18	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_PTC_C12_Basal_R2_12C_DnCl_SZ_12042024_18.mzML
SUBJECT_SAMPLE_FACTORS           	-	Pool_12C_DnCl_2_SZ_12042024_19	Sample source:Pool_12C | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=Pool_12C_DnCl_2_SZ_12042024_19.mzML
SUBJECT_SAMPLE_FACTORS           	-	Pool_12C13C_DnCl_2_SZ_12042024_20	Sample source:Pool_12C13C | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=Pool_12C13C_DnCl_2_SZ_12042024_20.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_CE_B08_DHEAS_R2_12C_DnCl_SZ_12042024_21	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_CE_B08_DHEAS_R2_12C_DnCl_SZ_12042024_21.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_PTC_F10_DHEAS_R3_12C_DnCl_SZ_12042024_22	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_PTC_F10_DHEAS_R3_12C_DnCl_SZ_12042024_22.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_PTC_F10_Basal_R1_12C_DnCl_SZ_12042024_23	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_PTC_F10_Basal_R1_12C_DnCl_SZ_12042024_23.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_PTC_D10_DHEAS_R2_12C_DnCl_SZ_12042024_24	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_PTC_D10_DHEAS_R2_12C_DnCl_SZ_12042024_24.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_KO_B03_Basal_R1_12C_DnCl_SZ_12042024_25	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_KO_B03_Basal_R1_12C_DnCl_SZ_12042024_25.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_CE_C05_DHEAS_R2_12C_DnCl_SZ_12042024_26	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_CE_C05_DHEAS_R2_12C_DnCl_SZ_12042024_26.mzML
SUBJECT_SAMPLE_FACTORS           	-	HSD3B1_PTC_C07_DHEAS_R1_12C_DnCl_SZ_12042024_27	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=HSD3B1_PTC_C07_DHEAS_R1_12C_DnCl_SZ_12042024_27.mzML
SUBJECT_SAMPLE_FACTORS           	-	HSD3B1_PTC_C12_Basal_R3_12C_DnCl_SZ_12042024_28	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=HSD3B1_PTC_C12_Basal_R3_12C_DnCl_SZ_12042024_28.mzML
SUBJECT_SAMPLE_FACTORS           	-	HSD3B1_PTC_C07_Basal_R1_12C_DnCl_SZ_12042024_29	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=HSD3B1_PTC_C07_Basal_R1_12C_DnCl_SZ_12042024_29.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_CE_B08_Basal_R1_12C_DnCl_SZ_12042024_30	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_CE_B08_Basal_R1_12C_DnCl_SZ_12042024_30.mzML
SUBJECT_SAMPLE_FACTORS           	-	Pool_12C_DnCl_3_SZ_12042024_31	Sample source:Pool_12C | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=Pool_12C_DnCl_3_SZ_12042024_31.mzML
SUBJECT_SAMPLE_FACTORS           	-	Pool_12C13C_DnCl_3_SZ_12042024_32	Sample source:Pool_12C13C | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=Pool_12C13C_DnCl_3_SZ_12042024_32.mzML
SUBJECT_SAMPLE_FACTORS           	-	HSD3B1_PTC_C12_DHEAS_R3_12C_DnCl_SZ_12042024_33	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=HSD3B1_PTC_C12_DHEAS_R3_12C_DnCl_SZ_12042024_33.mzML
SUBJECT_SAMPLE_FACTORS           	-	HSD3B1_PTC_D03_DHEAS_R2_12C_DnCl_SZ_12042024_34	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=HSD3B1_PTC_D03_DHEAS_R2_12C_DnCl_SZ_12042024_34.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_CE_A06_DHEAS_R1_12C_DnCl_SZ_12042024_35	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_CE_A06_DHEAS_R1_12C_DnCl_SZ_12042024_35.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_CE_C05_Basal_R3_12C_DnCl_SZ_12042024_36	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_CE_C05_Basal_R3_12C_DnCl_SZ_12042024_36.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_PTC_D10_Basal_R1_12C_DnCl_SZ_12042024_37	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_PTC_D10_Basal_R1_12C_DnCl_SZ_12042024_37.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_PTC_C12_DHEAS_R3_12C_DnCl_SZ_12042024_38	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_PTC_C12_DHEAS_R3_12C_DnCl_SZ_12042024_38.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_CE_A06_DHEAS_R2_12C_DnCl_SZ_12042024_39	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_CE_A06_DHEAS_R2_12C_DnCl_SZ_12042024_39.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_PTC_F10_DHEAS_R2_12C_DnCl_SZ_12042024_40	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_PTC_F10_DHEAS_R2_12C_DnCl_SZ_12042024_40.mzML
SUBJECT_SAMPLE_FACTORS           	-	HSD3B1_PTC_C07_DHEAS_R2_12C_DnCl_SZ_12042024_41	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=HSD3B1_PTC_C07_DHEAS_R2_12C_DnCl_SZ_12042024_41.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_PTC_C12_Basal_R1_12C_DnCl_SZ_12042024_42	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_PTC_C12_Basal_R1_12C_DnCl_SZ_12042024_42.mzML
SUBJECT_SAMPLE_FACTORS           	-	Pool_12C_DnCl_4_SZ_12042024_43	Sample source:Pool_12C | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=Pool_12C_DnCl_4_SZ_12042024_43.mzML
SUBJECT_SAMPLE_FACTORS           	-	Pool_12C13C_DnCl_4_SZ_12042024_44	Sample source:Pool_12C13C | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=Pool_12C13C_DnCl_4_SZ_12042024_44.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_PTC_C12_DHEAS_R2_12C_DnCl_SZ_12042024_45	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_PTC_C12_DHEAS_R2_12C_DnCl_SZ_12042024_45.mzML
SUBJECT_SAMPLE_FACTORS           	-	WT_Basal_2_12C_DnCl_SZ_12042024_46	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=WT_Basal_2_12C_DnCl_SZ_12042024_46.mzML
SUBJECT_SAMPLE_FACTORS           	-	HSD3B1_PTC_C12_DHEAS_R2_12C_DnCl_SZ_12042024_47	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=HSD3B1_PTC_C12_DHEAS_R2_12C_DnCl_SZ_12042024_47.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_CE_A06_Basal_R3_12C_DnCl_SZ_12042024_48	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_CE_A06_Basal_R3_12C_DnCl_SZ_12042024_48.mzML
SUBJECT_SAMPLE_FACTORS           	-	WT_DHEAS_3_12C_DnCl_SZ_12042024_49	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=WT_DHEAS_3_12C_DnCl_SZ_12042024_49.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_PTC_D10_DHEAS_R1_12C_DnCl_SZ_12042024_50	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_PTC_D10_DHEAS_R1_12C_DnCl_SZ_12042024_50.mzML
SUBJECT_SAMPLE_FACTORS           	-	HSD3B1_PTC_D03_Basal_R2_12C_DnCl_SZ_12042024_51	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=HSD3B1_PTC_D03_Basal_R2_12C_DnCl_SZ_12042024_51.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_CE_C05_Basal_R1_12C_DnCl_SZ_12042024_52	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_CE_C05_Basal_R1_12C_DnCl_SZ_12042024_52.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_CE_B08_Basal_R3_12C_DnCl_SZ_12042024_53	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_CE_B08_Basal_R3_12C_DnCl_SZ_12042024_53.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_CE_B08_DHEAS_R3_12C_DnCl_SZ_12042024_54	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_CE_B08_DHEAS_R3_12C_DnCl_SZ_12042024_54.mzML
SUBJECT_SAMPLE_FACTORS           	-	Pool_12C_DnCl_5_SZ_12042024_55	Sample source:Pool_12C | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=Pool_12C_DnCl_5_SZ_12042024_55.mzML
SUBJECT_SAMPLE_FACTORS           	-	Pool_12C13C_DnCl_5_SZ_12042024_56	Sample source:Pool_12C13C | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=Pool_12C13C_DnCl_5_SZ_12042024_56.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_PTC_D10_Basal_R3_12C_DnCl_SZ_12042024_57	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_PTC_D10_Basal_R3_12C_DnCl_SZ_12042024_57.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_PTC_F10_Basal_R2_12C_DnCl_SZ_12042024_58	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_PTC_F10_Basal_R2_12C_DnCl_SZ_12042024_58.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_KO_B03_DHEAS_R2_12C_DnCl_SZ_12042024_59	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_KO_B03_DHEAS_R2_12C_DnCl_SZ_12042024_59.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_KO_A01_DHEAS_R3_12C_DnCl_SZ_12042024_60	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_KO_A01_DHEAS_R3_12C_DnCl_SZ_12042024_60.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_KO_B03_Basal_R3_12C_DnCl_SZ_12042024_61	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_KO_B03_Basal_R3_12C_DnCl_SZ_12042024_61.mzML
SUBJECT_SAMPLE_FACTORS           	-	HSD3B1_PTC_C07_Basal_R3_12C_DnCl_SZ_12042024_62	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=HSD3B1_PTC_C07_Basal_R3_12C_DnCl_SZ_12042024_62.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_KO_A01_Basal_R3_12C_DnCl_SZ_12042024_63	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_KO_A01_Basal_R3_12C_DnCl_SZ_12042024_63.mzML
SUBJECT_SAMPLE_FACTORS           	-	HSD3B1_PTC_C12_Basal_R2_12C_DnCl_SZ_12042024_64	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=HSD3B1_PTC_C12_Basal_R2_12C_DnCl_SZ_12042024_64.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_CE_C05_DHEAS_R3_12C_DnCl_SZ_12042024_65	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_CE_C05_DHEAS_R3_12C_DnCl_SZ_12042024_65.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_KO_A02_Basal_R2_12C_DnCl_SZ_12042024_66	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_KO_A02_Basal_R2_12C_DnCl_SZ_12042024_66.mzML
SUBJECT_SAMPLE_FACTORS           	-	Pool_12C_DnCl_6_SZ_12042024_67	Sample source:Pool_12C | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=Pool_12C_DnCl_6_SZ_12042024_67.mzML
SUBJECT_SAMPLE_FACTORS           	-	Pool_12C13C_DnCl_6_SZ_12042024_68	Sample source:Pool_12C13C | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=Pool_12C13C_DnCl_6_SZ_12042024_68.mzML
SUBJECT_SAMPLE_FACTORS           	-	HSD3B1_PTC_D03_DHEAS_R1_12C_DnCl_SZ_12042024_69	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=HSD3B1_PTC_D03_DHEAS_R1_12C_DnCl_SZ_12042024_69.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_KO_A02_DHEAS_R1_12C_DnCl_SZ_12042024_70	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_KO_A02_DHEAS_R1_12C_DnCl_SZ_12042024_70.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_PTC_F10_DHEAS_R1_12C_DnCl_SZ_12042024_71	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_PTC_F10_DHEAS_R1_12C_DnCl_SZ_12042024_71.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_PTC_D10_DHEAS_R3_12C_DnCl_SZ_12042024_72	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_PTC_D10_DHEAS_R3_12C_DnCl_SZ_12042024_72.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_KO_A02_Basal_R3_12C_DnCl_SZ_12042024_73	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_KO_A02_Basal_R3_12C_DnCl_SZ_12042024_73.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_PTC_D10_Basal_R2_12C_DnCl_SZ_12042024_74	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_PTC_D10_Basal_R2_12C_DnCl_SZ_12042024_74.mzML
SUBJECT_SAMPLE_FACTORS           	-	HSD3B1_PTC_D03_Basal_R3_12C_DnCl_SZ_12042024_75	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=HSD3B1_PTC_D03_Basal_R3_12C_DnCl_SZ_12042024_75.mzML
SUBJECT_SAMPLE_FACTORS           	-	WT_Basal_3_12C_DnCl_SZ_12042024_76	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=WT_Basal_3_12C_DnCl_SZ_12042024_76.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_PTC_C12_Basal_R3_12C_DnCl_SZ_12042024_77	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_PTC_C12_Basal_R3_12C_DnCl_SZ_12042024_77.mzML
SUBJECT_SAMPLE_FACTORS           	-	HSD3B1_PTC_C07_DHEAS_R3_12C_DnCl_SZ_12042024_78	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=HSD3B1_PTC_C07_DHEAS_R3_12C_DnCl_SZ_12042024_78.mzML
SUBJECT_SAMPLE_FACTORS           	-	Pool_12C_DnCl_7_SZ_12042024_79	Sample source:Pool_12C | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=Pool_12C_DnCl_7_SZ_12042024_79.mzML
SUBJECT_SAMPLE_FACTORS           	-	Pool_12C13C_DnCl_7_SZ_12042024_80	Sample source:Pool_12C13C | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=Pool_12C13C_DnCl_7_SZ_12042024_80.mzML
SUBJECT_SAMPLE_FACTORS           	-	WT_DHEAS_2_12C_DnCl_SZ_12042024_81	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=WT_DHEAS_2_12C_DnCl_SZ_12042024_81.mzML
SUBJECT_SAMPLE_FACTORS           	-	HSD3B1_PTC_C07_Basal_R2_12C_DnCl_SZ_12042024_82	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=HSD3B1_PTC_C07_Basal_R2_12C_DnCl_SZ_12042024_82.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_KO_A01_DHEAS_R1_12C_DnCl_SZ_12042024_83	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_KO_A01_DHEAS_R1_12C_DnCl_SZ_12042024_83.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_PTC_C12_DHEAS_R1_12C_DnCl_SZ_12042024_84	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_PTC_C12_DHEAS_R1_12C_DnCl_SZ_12042024_84.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_KO_B03_Basal_R2_12C_DnCl_SZ_12042024_85	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_KO_B03_Basal_R2_12C_DnCl_SZ_12042024_85.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_KO_A02_DHEAS_R3_12C_DnCl_SZ_12042024_86	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_KO_A02_DHEAS_R3_12C_DnCl_SZ_12042024_86.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_CE_A06_DHEAS_R3_12C_DnCl_SZ_12042024_87	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_CE_A06_DHEAS_R3_12C_DnCl_SZ_12042024_87.mzML
SUBJECT_SAMPLE_FACTORS           	-	HSD3B1_PTC_C12_Basal_R1_12C_DnCl_SZ_12042024_88	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=HSD3B1_PTC_C12_Basal_R1_12C_DnCl_SZ_12042024_88.mzML
SUBJECT_SAMPLE_FACTORS           	-	HSD3B1_PTC_D03_DHEAS_R3_12C_DnCl_SZ_12042024_89	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=HSD3B1_PTC_D03_DHEAS_R3_12C_DnCl_SZ_12042024_89.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_KO_A01_Basal_R2_12C_DnCl_SZ_12042024_90	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_KO_A01_Basal_R2_12C_DnCl_SZ_12042024_90.mzML
SUBJECT_SAMPLE_FACTORS           	-	Pool_12C_DnCl_8_SZ_12042024_91	Sample source:Pool_12C | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=Pool_12C_DnCl_8_SZ_12042024_91.mzML
SUBJECT_SAMPLE_FACTORS           	-	Pool_12C13C_DnCl_8_SZ_12042024_92	Sample source:Pool_12C13C | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=Pool_12C13C_DnCl_8_SZ_12042024_92.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_CE_C05_Basal_R2_12C_DnCl_SZ_12042024_93	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_CE_C05_Basal_R2_12C_DnCl_SZ_12042024_93.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_KO_B03_DHEAS_R3_12C_DnCl_SZ_12042024_94	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_KO_B03_DHEAS_R3_12C_DnCl_SZ_12042024_94.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_CE_B08_Basal_R2_12C_DnCl_SZ_12042024_95	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_CE_B08_Basal_R2_12C_DnCl_SZ_12042024_95.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_PTC_F10_Basal_R3_12C_DnCl_SZ_12042024_96	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_PTC_F10_Basal_R3_12C_DnCl_SZ_12042024_96.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_CE_C05_DHEAS_R1_12C_DnCl_SZ_12042024_97	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_CE_C05_DHEAS_R1_12C_DnCl_SZ_12042024_97.mzML
SUBJECT_SAMPLE_FACTORS           	-	HSD3B1_PTC_C12_DHEAS_R1_12C_DnCl_SZ_12042024_98	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=HSD3B1_PTC_C12_DHEAS_R1_12C_DnCl_SZ_12042024_98.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_CE_B08_DHEAS_R1_12C_DnCl_SZ_12042024_99	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_CE_B08_DHEAS_R1_12C_DnCl_SZ_12042024_99.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_CE_A06_Basal_R2_12C_DnCl_SZ_12042024_100	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_CE_A06_Basal_R2_12C_DnCl_SZ_12042024_100.mzML
SUBJECT_SAMPLE_FACTORS           	-	Pool_12C_DnCl_9_SZ_12042024_101	Sample source:Pool_12C | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=Pool_12C_DnCl_9_SZ_12042024_101.mzML
SUBJECT_SAMPLE_FACTORS           	-	Pool_12C13C_DnCl_9_SZ_12042024_102	Sample source:Pool_12C13C | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=Pool_12C13C_DnCl_9_SZ_12042024_102.mzML
SUBJECT_SAMPLE_FACTORS           	-	Qstd_2_SZ_12042024_103	Sample source:Qstd | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=Qstd_2_SZ_12042024_103.mzML
SUBJECT_SAMPLE_FACTORS           	-	Standard_12C_DnCl_2_SZ_12042024_104	Sample source:Standard_12C | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=Standard_12C_DnCl_2_SZ_12042024_104.mzML
SUBJECT_SAMPLE_FACTORS           	-	Pool_no_DnCl_2_SZ_12042024_105	Sample source:Pooled_Underivatized | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=Pool_no_DnCl_2_SZ_12042024_105.mzML
SUBJECT_SAMPLE_FACTORS           	-	Process_Blank_12C13C_DnCl_2_SZ_12042024_106	Sample source:Process_Blank | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=Process_Blank_12C13C_DnCl_2_SZ_12042024_106.mzML
SUBJECT_SAMPLE_FACTORS           	-	Process_Blank_12C_DnCl_2_SZ_12042024_107	Sample source:Process_Blank | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=Process_Blank_12C_DnCl_2_SZ_12042024_107.mzML
SUBJECT_SAMPLE_FACTORS           	-	Solvent_Blank_2_SZ_12042024_108	Sample source:Solvent_Blank | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=Solvent_Blank_2_SZ_12042024_108.mzML
SUBJECT_SAMPLE_FACTORS           	-	Solvent_Blank_1_SZ_12062024_01	Sample source:Solvent_Blank | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=Solvent_Blank_1_SZ_12062024_01.mzML
SUBJECT_SAMPLE_FACTORS           	-	Process_Blank_12C_DnHz_1_SZ_12062024_02	Sample source:Process_Blank | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=Process_Blank_12C_DnHz_1_SZ_12062024_02.mzML
SUBJECT_SAMPLE_FACTORS           	-	Process_Blank_12C13C_DnHz_SZ_12062024_03	Sample source:Process_Blank | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=Process_Blank_12C13C_DnHz_SZ_12062024_03.mzML
SUBJECT_SAMPLE_FACTORS           	-	Pool_no_DnHz_1_12062024_04	Sample source:Pooled_Underivatized | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=Pool_no_DnHz_1_12062024_04.mzML
SUBJECT_SAMPLE_FACTORS           	-	Standard_12C_DnHz_1_SZ_12062024_05	Sample source:Standard_12C | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=Standard_12C_DnHz_1_SZ_12062024_05.mzML
SUBJECT_SAMPLE_FACTORS           	-	Pool_12C_DnHz_1_SZ_12062024_06	Sample source:Pool_12C | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=Pool_12C_DnHz_1_SZ_12062024_06.mzML
SUBJECT_SAMPLE_FACTORS           	-	Pool_12C13C_DnHz_1_SZ_12062024_07	Sample source:Pool_12C13C | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=Pool_12C13C_DnHz_1_SZ_12062024_07.mzML
SUBJECT_SAMPLE_FACTORS           	-	Qstd_1_SZ_12062024_08	Sample source:Qstd | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=Qstd_1_SZ_12062024_08.mzML
SUBJECT_SAMPLE_FACTORS           	-	HSD3B1_PTC_D03_Basal_R2_12C_DnHz_SZ_12062024_09	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=HSD3B1_PTC_D03_Basal_R2_12C_DnHz_SZ_12062024_09.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_KO_B03_Basal_R3_12C_DnHz_SZ_12062024_10	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_KO_B03_Basal_R3_12C_DnHz_SZ_12062024_10.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_KO_A01_Basal_R1_12C_DnHz_SZ_12062024_11	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_KO_A01_Basal_R1_12C_DnHz_SZ_12062024_11.mzML
SUBJECT_SAMPLE_FACTORS           	-	HSD3B1_PTC_C07_Basal_R1_12C_DnHz_SZ_12062024_12	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=HSD3B1_PTC_C07_Basal_R1_12C_DnHz_SZ_12062024_12.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_PTC_C12_DHEAS_R1_12C_DnHz_SZ_12062024_13	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_PTC_C12_DHEAS_R1_12C_DnHz_SZ_12062024_13.mzML
SUBJECT_SAMPLE_FACTORS           	-	HSD3B1_PTC_C12_Basal_R1_12C_DnHz_SZ_12062024_14	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=HSD3B1_PTC_C12_Basal_R1_12C_DnHz_SZ_12062024_14.mzML
SUBJECT_SAMPLE_FACTORS           	-	WT_Basal_2_12C_DnHz_SZ_12062024_15	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=WT_Basal_2_12C_DnHz_SZ_12062024_15.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_KO_A02_Basal_R2_12C_DnHz_SZ_12062024_16	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_KO_A02_Basal_R2_12C_DnHz_SZ_12062024_16.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_PTC_F10_DHEAS_R2_12C_DnHz_SZ_12062024_17	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_PTC_F10_DHEAS_R2_12C_DnHz_SZ_12062024_17.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_KO_A02_DHEAS_R3_12C_DnHz_SZ_12062024_18	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_KO_A02_DHEAS_R3_12C_DnHz_SZ_12062024_18.mzML
SUBJECT_SAMPLE_FACTORS           	-	Pool_12C_DnHz_2_SZ_12062024_19	Sample source:Pool_12C | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=Pool_12C_DnHz_2_SZ_12062024_19.mzML
SUBJECT_SAMPLE_FACTORS           	-	Pool_12C13C_DnHz_2_SZ_12062024_20	Sample source:Pool_12C13C | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=Pool_12C13C_DnHz_2_SZ_12062024_20.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_CE_A06_Basal_R3_12C_DnHz_SZ_12062024_21	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_CE_A06_Basal_R3_12C_DnHz_SZ_12062024_21.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_CE_B08_DHEAS_R3_12C_DnHz_SZ_12062024_22	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_CE_B08_DHEAS_R3_12C_DnHz_SZ_12062024_22.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_PTC_D10_DHEAS_R2_12C_DnHz_SZ_12062024_23	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_PTC_D10_DHEAS_R2_12C_DnHz_SZ_12062024_23.mzML
SUBJECT_SAMPLE_FACTORS           	-	HSD3B1_PTC_C07_DHEAS_R1_12C_DnHz_SZ_12062024_24	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=HSD3B1_PTC_C07_DHEAS_R1_12C_DnHz_SZ_12062024_24.mzML
SUBJECT_SAMPLE_FACTORS           	-	HSD3B1_PTC_C12_DHEAS_R2_12C_DnHz_SZ_12062024_25	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=HSD3B1_PTC_C12_DHEAS_R2_12C_DnHz_SZ_12062024_25.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_CE_C05_Basal_R2_12C_DnHz_SZ_12062024_26	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_CE_C05_Basal_R2_12C_DnHz_SZ_12062024_26.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_CE_B08_Basal_R3_12C_DnHz_SZ_12062024_27	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_CE_B08_Basal_R3_12C_DnHz_SZ_12062024_27.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_PTC_D10_Basal_R3_12C_DnHz_SZ_12062024_28	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_PTC_D10_Basal_R3_12C_DnHz_SZ_12062024_28.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_KO_B03_DHEAS_R1_12C_DnHz_SZ_12062024_29	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_KO_B03_DHEAS_R1_12C_DnHz_SZ_12062024_29.mzML
SUBJECT_SAMPLE_FACTORS           	-	WT_DHEAS_2_12C_DnHz_SZ_12062024_30	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=WT_DHEAS_2_12C_DnHz_SZ_12062024_30.mzML
SUBJECT_SAMPLE_FACTORS           	-	Pool_12C_DnHz_3_SZ_12062024_31	Sample source:Pool_12C | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=Pool_12C_DnHz_3_SZ_12062024_31.mzML
SUBJECT_SAMPLE_FACTORS           	-	Pool_12C13C_DnHz_3_SZ_12062024_32	Sample source:Pool_12C13C | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=Pool_12C13C_DnHz_3_SZ_12062024_32.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_CE_A06_DHEAS_R2_12C_DnHz_SZ_12062024_33	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_CE_A06_DHEAS_R2_12C_DnHz_SZ_12062024_33.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_KO_A01_DHEAS_R2_12C_DnHz_SZ_12062024_34	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_KO_A01_DHEAS_R2_12C_DnHz_SZ_12062024_34.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_PTC_C12_Basal_R3_12C_DnHz_SZ_12062024_35	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_PTC_C12_Basal_R3_12C_DnHz_SZ_12062024_35.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_PTC_F10_Basal_R1_12C_DnHz_SZ_12062024_36	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_PTC_F10_Basal_R1_12C_DnHz_SZ_12062024_36.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_CE_C05_DHEAS_R3_12C_DnHz_SZ_12062024_37	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_CE_C05_DHEAS_R3_12C_DnHz_SZ_12062024_37.mzML
SUBJECT_SAMPLE_FACTORS           	-	HSD3B1_PTC_D03_DHEAS_R3_12C_DnHz_SZ_12062024_38	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=HSD3B1_PTC_D03_DHEAS_R3_12C_DnHz_SZ_12062024_38.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_PTC_C12_DHEAS_R2_12C_DnHz_SZ_12062024_39	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_PTC_C12_DHEAS_R2_12C_DnHz_SZ_12062024_39.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_KO_A01_Basal_R2_12C_DnHz_SZ_12062024_40	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_KO_A01_Basal_R2_12C_DnHz_SZ_12062024_40.mzML
SUBJECT_SAMPLE_FACTORS           	-	HSD3B1_PTC_C12_DHEAS_R1_12C_DnHz_SZ_12062024_41	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=HSD3B1_PTC_C12_DHEAS_R1_12C_DnHz_SZ_12062024_41.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_PTC_F10_Basal_R3_12C_DnHz_SZ_12062024_42	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_PTC_F10_Basal_R3_12C_DnHz_SZ_12062024_42.mzML
SUBJECT_SAMPLE_FACTORS           	-	Pool_12C_DnHz_4_SZ_12062024_43	Sample source:Pool_12C | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=Pool_12C_DnHz_4_SZ_12062024_43.mzML
SUBJECT_SAMPLE_FACTORS           	-	Pool_12C13C_DnHz_4_SZ_12062024_44	Sample source:Pool_12C13C | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=Pool_12C13C_DnHz_4_SZ_12062024_44.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_KO_B03_DHEAS_R2_12C_DnHz_SZ_12062024_45	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_KO_B03_DHEAS_R2_12C_DnHz_SZ_12062024_45.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_CE_C05_Basal_R3_12C_DnHz_SZ_12062024_46	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_CE_C05_Basal_R3_12C_DnHz_SZ_12062024_46.mzML
SUBJECT_SAMPLE_FACTORS           	-	HSD3B1_PTC_D03_Basal_R3_12C_DnHz_SZ_12062024_47	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=HSD3B1_PTC_D03_Basal_R3_12C_DnHz_SZ_12062024_47.mzML
SUBJECT_SAMPLE_FACTORS           	-	HSD3B1_PTC_C12_Basal_R3_12C_DnHz_SZ_12062024_48	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=HSD3B1_PTC_C12_Basal_R3_12C_DnHz_SZ_12062024_48.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_CE_A06_DHEAS_R3_12C_DnHz_SZ_12062024_49	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_CE_A06_DHEAS_R3_12C_DnHz_SZ_12062024_49.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_KO_A02_DHEAS_R2_12C_DnHz_SZ_12062024_50	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_KO_A02_DHEAS_R2_12C_DnHz_SZ_12062024_50.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_KO_B03_Basal_R2_12C_DnHz_SZ_12062024_51	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_KO_B03_Basal_R2_12C_DnHz_SZ_12062024_51.mzML
SUBJECT_SAMPLE_FACTORS           	-	WT_Basal_3_12C_DnHz_SZ_12062024_52	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=WT_Basal_3_12C_DnHz_SZ_12062024_52.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_KO_A02_Basal_R1_12C_DnHz_SZ_12062024_53	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_KO_A02_Basal_R1_12C_DnHz_SZ_12062024_53.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_CE_C05_DHEAS_R1_12C_DnHz_SZ_12062024_54	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_CE_C05_DHEAS_R1_12C_DnHz_SZ_12062024_54.mzML
SUBJECT_SAMPLE_FACTORS           	-	Pool_12C_DnHz_5_SZ_12062024_55	Sample source:Pool_12C | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=Pool_12C_DnHz_5_SZ_12062024_55.mzML
SUBJECT_SAMPLE_FACTORS           	-	Pool_12C13C_DnHz_5_SZ_12062024_56	Sample source:Pool_12C13C | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=Pool_12C13C_DnHz_5_SZ_12062024_56.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_CE_B08_DHEAS_R2_12C_DnHz_SZ_12062024_57	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_CE_B08_DHEAS_R2_12C_DnHz_SZ_12062024_57.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_PTC_C12_Basal_R1_12C_DnHz_SZ_12062024_58	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_PTC_C12_Basal_R1_12C_DnHz_SZ_12062024_58.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_PTC_D10_DHEAS_R3_12C_DnHz_SZ_12062024_59	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_PTC_D10_DHEAS_R3_12C_DnHz_SZ_12062024_59.mzML
SUBJECT_SAMPLE_FACTORS           	-	HSD3B1_PTC_C07_DHEAS_R3_12C_DnHz_SZ_12062024_60	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=HSD3B1_PTC_C07_DHEAS_R3_12C_DnHz_SZ_12062024_60.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_KO_A01_DHEAS_R1_12C_DnHz_SZ_12062024_61	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_KO_A01_DHEAS_R1_12C_DnHz_SZ_12062024_61.mzML
SUBJECT_SAMPLE_FACTORS           	-	HSD3B1_PTC_C07_Basal_R2_12C_DnHz_SZ_12062024_62	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=HSD3B1_PTC_C07_Basal_R2_12C_DnHz_SZ_12062024_62.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_CE_A06_Basal_R2_12C_DnHz_SZ_12062024_63	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_CE_A06_Basal_R2_12C_DnHz_SZ_12062024_63.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_PTC_D10_Basal_R2_12C_DnHz_SZ_12062024_64	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_PTC_D10_Basal_R2_12C_DnHz_SZ_12062024_64.mzML
SUBJECT_SAMPLE_FACTORS           	-	WT_DHEAS_3_12C_DnHz_SZ_12062024_65	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=WT_DHEAS_3_12C_DnHz_SZ_12062024_65.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_CE_B08_Basal_R2_12C_DnHz_SZ_12062024_66	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_CE_B08_Basal_R2_12C_DnHz_SZ_12062024_66.mzML
SUBJECT_SAMPLE_FACTORS           	-	Pool_12C_DnHz_6_SZ_12062024_67	Sample source:Pool_12C | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=Pool_12C_DnHz_6_SZ_12062024_67.mzML
SUBJECT_SAMPLE_FACTORS           	-	Pool_12C13C_DnHz_6_SZ_12062024_68	Sample source:Pool_12C13C | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=Pool_12C13C_DnHz_6_SZ_12062024_68.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_PTC_F10_DHEAS_R3_12C_DnHz_SZ_12062024_69	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_PTC_F10_DHEAS_R3_12C_DnHz_SZ_12062024_69.mzML
SUBJECT_SAMPLE_FACTORS           	-	HSD3B1_PTC_D03_DHEAS_R1_12C_DnHz_SZ_12062024_70	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=HSD3B1_PTC_D03_DHEAS_R1_12C_DnHz_SZ_12062024_70.mzML
SUBJECT_SAMPLE_FACTORS           	-	WT_Basal_1_12C_DnHz_SZ_12062024_71	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=WT_Basal_1_12C_DnHz_SZ_12062024_71.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_KO_A01_Basal_R3_12C_DnHz_SZ_12062024_72	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_KO_A01_Basal_R3_12C_DnHz_SZ_12062024_72.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_KO_A02_DHEAS_R1_12C_DnHz_SZ_12062024_73	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_KO_A02_DHEAS_R1_12C_DnHz_SZ_12062024_73.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_KO_A02_Basal_R3_12C_DnHz_SZ_12062024_74	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_KO_A02_Basal_R3_12C_DnHz_SZ_12062024_74.mzML
SUBJECT_SAMPLE_FACTORS           	-	HSD3B1_PTC_C12_Basal_R2_12C_DnHz_SZ_12062024_75	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=HSD3B1_PTC_C12_Basal_R2_12C_DnHz_SZ_12062024_75.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_PTC_C12_Basal_R2_12C_DnHz_SZ_12062024_76	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_PTC_C12_Basal_R2_12C_DnHz_SZ_12062024_76.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_CE_C05_Basal_R1_12C_DnHz_SZ_12062024_77	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_CE_C05_Basal_R1_12C_DnHz_SZ_12062024_77.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_CE_A06_Basal_R1_12C_DnHz_SZ_12062024_78	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_CE_A06_Basal_R1_12C_DnHz_SZ_12062024_78.mzML
SUBJECT_SAMPLE_FACTORS           	-	Pool_12C_DnHz_7_SZ_12062024_79	Sample source:Pool_12C | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=Pool_12C_DnHz_7_SZ_12062024_79.mzML
SUBJECT_SAMPLE_FACTORS           	-	Pool_12C13C_DnHz_7_SZ_12062024_80	Sample source:Pool_12C13C | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=Pool_12C13C_DnHz_7_SZ_12062024_80.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_CE_C05_DHEAS_R2_12C_DnHz_SZ_12062024_81	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_CE_C05_DHEAS_R2_12C_DnHz_SZ_12062024_81.mzML
SUBJECT_SAMPLE_FACTORS           	-	WT_DHEAS_1_12C_DnHz_SZ_12062024_82	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=WT_DHEAS_1_12C_DnHz_SZ_12062024_82.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_PTC_D10_DHEAS_R1_12C_DnHz_SZ_12062024_83	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_PTC_D10_DHEAS_R1_12C_DnHz_SZ_12062024_83.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_PTC_C12_DHEAS_R3_12C_DnHz_SZ_12062024_84	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_PTC_C12_DHEAS_R3_12C_DnHz_SZ_12062024_84.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_KO_B03_Basal_R1_12C_DnHz_SZ_12062024_85	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_KO_B03_Basal_R1_12C_DnHz_SZ_12062024_85.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_CE_A06_DHEAS_R1_12C_DnHz_SZ_12062024_86	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_CE_A06_DHEAS_R1_12C_DnHz_SZ_12062024_86.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_PTC_D10_Basal_R1_12C_DnHz_SZ_12062024_87	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_PTC_D10_Basal_R1_12C_DnHz_SZ_12062024_87.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_PTC_F10_DHEAS_R1_12C_DnHz_SZ_12062024_88	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_PTC_F10_DHEAS_R1_12C_DnHz_SZ_12062024_88.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_KO_B03_DHEAS_R3_12C_DnHz_SZ_12062024_89	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_KO_B03_DHEAS_R3_12C_DnHz_SZ_12062024_89.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_CE_B08_DHEAS_R1_12C_DnHz_SZ_12062024_90	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_CE_B08_DHEAS_R1_12C_DnHz_SZ_12062024_90.mzML
SUBJECT_SAMPLE_FACTORS           	-	Pool_12C_DnHz_8_SZ_12062024_91	Sample source:Pool_12C | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=Pool_12C_DnHz_8_SZ_12062024_91.mzML
SUBJECT_SAMPLE_FACTORS           	-	Pool_12C13C_DnHz_8_SZ_12062024_92	Sample source:Pool_12C13C | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=Pool_12C13C_DnHz_8_SZ_12062024_92.mzML
SUBJECT_SAMPLE_FACTORS           	-	HSD3B1_PTC_C07_DHEAS_R2_12C_DnHz_SZ_12062024_93	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=HSD3B1_PTC_C07_DHEAS_R2_12C_DnHz_SZ_12062024_93.mzML
SUBJECT_SAMPLE_FACTORS           	-	HSD3B1_PTC_C12_DHEAS_R3_12C_DnHz_SZ_12062024_94	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=HSD3B1_PTC_C12_DHEAS_R3_12C_DnHz_SZ_12062024_94.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_KO_A01_DHEAS_R3_12C_DnHz_SZ_12062024_95	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_KO_A01_DHEAS_R3_12C_DnHz_SZ_12062024_95.mzML
SUBJECT_SAMPLE_FACTORS           	-	HSD3B1_PTC_D03_Basal_R1_12C_DnHz_SZ_12062024_96	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=HSD3B1_PTC_D03_Basal_R1_12C_DnHz_SZ_12062024_96.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_CE_B08_Basal_R1_12C_DnHz_SZ_12062024_97	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_CE_B08_Basal_R1_12C_DnHz_SZ_12062024_97.mzML
SUBJECT_SAMPLE_FACTORS           	-	HSD3B1_PTC_D03_DHEAS_R2_12C_DnHz_SZ_12062024_98	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=HSD3B1_PTC_D03_DHEAS_R2_12C_DnHz_SZ_12062024_98.mzML
SUBJECT_SAMPLE_FACTORS           	-	STS_PTC_F10_Basal_R2_12C_DnHz_SZ_12062024_99	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=STS_PTC_F10_Basal_R2_12C_DnHz_SZ_12062024_99.mzML
SUBJECT_SAMPLE_FACTORS           	-	HSD3B1_PTC_C07_Basal_R3_12C_DnHz_SZ_12062024_100	Sample source:Stem Cells Culture | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=HSD3B1_PTC_C07_Basal_R3_12C_DnHz_SZ_12062024_100.mzML
SUBJECT_SAMPLE_FACTORS           	-	Pool_12C_DnHz_9_SZ_12062024_101	Sample source:Pool_12C | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=Pool_12C_DnHz_9_SZ_12062024_101.mzML
SUBJECT_SAMPLE_FACTORS           	-	Pool_12C13C_DnHz_9_SZ_12062024_102	Sample source:Pool_12C13C | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=Pool_12C13C_DnHz_9_SZ_12062024_102.mzML
SUBJECT_SAMPLE_FACTORS           	-	Qstd_2_SZ_12062024_103	Sample source:Qstd | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=Qstd_2_SZ_12062024_103.mzML
SUBJECT_SAMPLE_FACTORS           	-	Standard_12C_DnHz_2_SZ_12062024_104	Sample source:Standard_12C | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=Standard_12C_DnHz_2_SZ_12062024_104.mzML
SUBJECT_SAMPLE_FACTORS           	-	Pool_no_DnHz_2_SZ_12062024_105	Sample source:Pooled_Underivatized | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=Pool_no_DnHz_2_SZ_12062024_105.mzML
SUBJECT_SAMPLE_FACTORS           	-	Process_Blank_12C13C_DnHz_2_SZ_12062024_106	Sample source:Process_Blank | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=Process_Blank_12C13C_DnHz_2_SZ_12062024_106.mzML
SUBJECT_SAMPLE_FACTORS           	-	Process_Blank_12C_DnHz_2_SZ_12062024_107	Sample source:Process_Blank | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=Process_Blank_12C_DnHz_2_SZ_12062024_107.mzML
SUBJECT_SAMPLE_FACTORS           	-	Solvent_Blank_2_SZ_12062024_108	Sample source:Solvent_Blank | Harvest:Supernatant	RAW_FILE_NAME(Raw file name)=Solvent_Blank_2_SZ_12062024_108.mzML
#COLLECTION
CO:COLLECTION_SUMMARY            	For the media collection, 500ul of media was directly aspirated into 1.5 ml
CO:COLLECTION_SUMMARY            	Eppendorf tube from each well and flash frozen with liquid nitrogen, stored
CO:COLLECTION_SUMMARY            	immediately at -80 degree C.
CO:SAMPLE_TYPE                   	iPSC cells
#TREATMENT
TR:TREATMENT_SUMMARY             	KOLF2.2J cells were passaged as single cells with Accutase and seeded onto
TR:TREATMENT_SUMMARY             	SynthemaxII coated 12-well plates at 10k cells per well. 24 h after cell
TR:TREATMENT_SUMMARY             	passage, StemFlex media was changed to trophectoderm induction media (TE media).
TR:TREATMENT_SUMMARY             	Basal TE media consists of DMEM/F12, supplemented with 20% KnockOut Serum
TR:TREATMENT_SUMMARY             	(ThermoFisher, #10828028), 2 mM L-Glutamine (Gibco™, #25030081), 1x MEM
TR:TREATMENT_SUMMARY             	non-essential amnio acid (GibcoTM, #11140050), 0.1 mM β-Mercaptoethanol (Sigma,
TR:TREATMENT_SUMMARY             	#3148). In addition, 100 ng/ml BMP4 (R&D, #314-BP- 050/CF), and 20 μM SU5402
TR:TREATMENT_SUMMARY             	(Millipore Sigma, # 57263) were added at day 0 and onwards to induce trophoblast
TR:TREATMENT_SUMMARY             	lineage (BS condition). For the induction of primitive syncytium, 1 mM A-83
TR:TREATMENT_SUMMARY             	(TOCRIS, #2939) was added at the end of Day 2. Media was refreshed every 2 days
TR:TREATMENT_SUMMARY             	until the end of differentiation (Day 6). On day 6, TE media was replaced by the
TR:TREATMENT_SUMMARY             	StemFlex media with or without addition of nutrients 24 hours prior to
TR:TREATMENT_SUMMARY             	collection.
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	See from protocol file.
SP:SAMPLEPREP_PROTOCOL_FILENAME  	OnePhaseSupernatant_Protocol_SZ_05132025.docx;
SP:SAMPLEPREP_PROTOCOL_FILENAME  	OnePhaseCellPellet_Protocol_SZ_05132025.docx;
SP:SAMPLEPREP_PROTOCOL_FILENAME  	DnHz_Protocol_one_phase_extraction_SZ_05132025.docx;
SP:SAMPLEPREP_PROTOCOL_FILENAME  	DnCl_Protocol_one_phase_extraction_SZ_05132025.docx
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_SUMMARY        	See protocol file
CH:CHROMATOGRAPHY_TYPE           	Reversed phase
CH:INSTRUMENT_NAME               	Thermo Vanquish Duo UHPLC
CH:COLUMN_NAME                   	Waters ACQUITY UPLC CSH C18 (100 x 2.1mm,1.7um)
CH:SOLVENT_A                     	95% water/5% acetonitrile; 0.1% Formic acid
CH:SOLVENT_B                     	100% acetonitrile; 0.1% Formic acid (v/v)
CH:FLOW_GRADIENT                 	0.0min: 100%B, 3.0min: 100%B, 16.0min: 65%B, 18.0min: 100%B, 21.0min: 100%B,
CH:FLOW_GRADIENT                 	21.5min: 0%B, 26.0min: 0%B.
CH:FLOW_RATE                     	0.4 mL/min
CH:COLUMN_TEMPERATURE            	45
CH:METHODS_FILENAME              	DnCl_DnHZ_LC_MS_Protocol_SZ_05132025.docx
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
AN:ANALYSIS_PROTOCOL_FILE        	DnCl_DnHZ_LC_MS_Protocol_SZ_05132025.docx.docx
#MS
MS:INSTRUMENT_NAME               	Thermo Q Exactive HF-X Orbitrap
MS:INSTRUMENT_TYPE               	Orbitrap
MS:MS_TYPE                       	ESI
MS:ION_MODE                      	POSITIVE
MS:MS_COMMENTS                   	See from Protocol file.
MS:MS_RESULTS_FILE               	ST003952_AN006496_Results.txt	UNITS:peak intensity	Has m/z:Yes	Has RT:Yes	RT units:Seconds
#END