#METABOLOMICS WORKBENCH imssr_20250602_213922 DATATRACK_ID:5984 STUDY_ID:ST003966 ANALYSIS_ID:AN006524 PROJECT_ID:PR002483
VERSION                          	1
CREATED_ON                       	06-17-2025
#PROJECT
PR:PROJECT_TITLE                 	Metabolomics of CAR-T cells and their products in leukemia patients
PR:PROJECT_TYPE                  	Untargeted metabolomics
PR:PROJECT_SUMMARY               	Although most patients with relapsed/refractory B-cell acute lymphoblastic
PR:PROJECT_SUMMARY               	leukemia (B-ALL) receiving CD19-targeted chimeric antigen receptor (CAR) T cell
PR:PROJECT_SUMMARY               	therapy achieve remission, loss of CAR T cell functionality and subsequent
PR:PROJECT_SUMMARY               	relapse remains an unmet therapeutic need. We applied an integrative approach to
PR:PROJECT_SUMMARY               	study the immunometabolism of pre- and post-infusion CD19-CAR T cells of
PR:PROJECT_SUMMARY               	patients with relapsed/refractory B-ALL. Pre-infusion CAR T cells of long-term
PR:PROJECT_SUMMARY               	responders (LTR) had increased oxidative phosphorylation, fatty acid oxidation,
PR:PROJECT_SUMMARY               	and pentose phosphate pathway activities, higher mitochondrial mass, tighter
PR:PROJECT_SUMMARY               	cristae, and lower mTOR expression compared to products of short-term
PR:PROJECT_SUMMARY               	responders. Post-infusion CAR T cells in bone marrow (BM) of LTR had high
PR:PROJECT_SUMMARY               	immunometabolic plasticity and mTOR-pS6 expression supported by the BM
PR:PROJECT_SUMMARY               	microenvironment. Transient inhibition of mTOR during manufacture induced
PR:PROJECT_SUMMARY               	metabolic reprogramming and enhanced anti-tumor activity of CAR T cells. Our
PR:PROJECT_SUMMARY               	findings provide insight into immunometabolic determinants of long-term response
PR:PROJECT_SUMMARY               	and suggest a therapeutic strategy to improve long-term remission.
PR:INSTITUTE                     	Translational Genomics Research Institute
PR:LAST_NAME                     	Pirrotte
PR:FIRST_NAME                    	Patrick
PR:ADDRESS                       	445 N 5TH STREET, Phoenix, Arizona
PR:EMAIL                         	ims@tgen.org
PR:PHONE                         	6023438454
PR:FUNDING_SOURCE                	NIH K12 grant no. 5K12CA001727–29, The Hyundai Hope on Wheels Young
PR:FUNDING_SOURCE                	Investigator Award, NCI grant no.P30CA033572
PR:PROJECT_COMMENTS              	Study 1 of 4
PR:DOI                           	http://dx.doi.org/10.21228/M8PJ91
PR:CONTRIBUTORS                  	Lior Goldberg, Eric R. Haas, Jiaqi Wu, Bryan Garcia, Ryan Urak, Vibhuti Vyas,
PR:CONTRIBUTORS                  	Ruby Espinosa, Tamara Munoz, Shirley Bierkatz, Jamie R. Wagner, Jinny Paul, Mary
PR:CONTRIBUTORS                  	C. Clark, Dat Ngo, Ibrahim Aldoss, Stephen J. Forman & Xiuli Wang
#STUDY
ST:STUDY_TITLE                   	Immunometabolic determinants of long-term response in bone marrow of the
ST:STUDY_TITLE                   	leukemia patients receiving CD19 CAR T cell therapy
ST:STUDY_TYPE                    	Untargeted metabolomics
ST:STUDY_SUMMARY                 	To study relationship between immunometabolism and CAR T cell anti-tumor
ST:STUDY_SUMMARY                 	activity in patients, we examined bone marrow samples 28th day post CAR-T cell
ST:STUDY_SUMMARY                 	treatment. By looking into patients' bone marrow metabolome using untargeted
ST:STUDY_SUMMARY                 	metabolomics allowed us to investigate the microenvironment of both leukemia and
ST:STUDY_SUMMARY                 	post-infusion CAR-T cells. We identified that bone marrow metabolome was
ST:STUDY_SUMMARY                 	significantly different between long-term and short-term responders where bone
ST:STUDY_SUMMARY                 	marrow of long-term responders had significantly higher amino acid pool, lactic
ST:STUDY_SUMMARY                 	acid and inosine compared to short-term responders further the CAR-T cells in
ST:STUDY_SUMMARY                 	bone marrow also had acquired distinct metabolic state post infusion. The study
ST:STUDY_SUMMARY                 	identified metabolic composition of microenvironment has significant role in
ST:STUDY_SUMMARY                 	efficacy of CAR-T therapy.
ST:INSTITUTE                     	Translational Genomics Research Institute
ST:LAST_NAME                     	Pirrotte
ST:FIRST_NAME                    	Patrick
ST:ADDRESS                       	445 N 5th St, Phoenix, Arizona
ST:EMAIL                         	ims@tgen.org
ST:PHONE                         	16023438454
ST:SUBMIT_DATE                   	2025-06-02
#SUBJECT
SU:SUBJECT_TYPE                  	Human
SU:SUBJECT_SPECIES               	Homo sapiens
SU:TAXONOMY_ID                   	9606
SU:GENDER                        	Male and female
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data
SUBJECT_SAMPLE_FACTORS           	188SBM2LTR	188SBM2LTR_HILICneg	Sample source:Bone marrow serum | Outcome:Long-term responders	RAW_FILE_NAME(File name)=160_0005_01A_R_188SBM2LTRxHILICneg_30AUG24_WAT_WAT_LUM_0000_18AB_15MIN_KP_KP_01.raw
SUBJECT_SAMPLE_FACTORS           	188SBM2LTR	188SBM2LTR_HILICpos	Sample source:Bone marrow serum | Outcome:Long-term responders	RAW_FILE_NAME(File name)=160_0005_01A_R_188SBM2LTRxHILICpos_30AUG24_WAT_WAT_LUM_0000_18AB_15MIN_KP_KP_01.raw
SUBJECT_SAMPLE_FACTORS           	188SBM2LTR	188SBM2LTR_RPneg	Sample source:Bone marrow serum | Outcome:Long-term responders	RAW_FILE_NAME(File name)=160_0005_01B_R_188SBM2LTRxRPneg_03SEP24_WAT_WAT_LUM_0000_18AC_15MIN_KP_KP_01.raw
SUBJECT_SAMPLE_FACTORS           	188SBM2LTR	188SBM2LTR_RPpos	Sample source:Bone marrow serum | Outcome:Long-term responders	RAW_FILE_NAME(File name)=160_0005_01B_R_188SBM2LTRxRPpos_03SEP24_WAT_WAT_LUM_0000_18AC_15MIN_KP_KP_01.raw
SUBJECT_SAMPLE_FACTORS           	245SBM3LTR	245SBM3LTR_HILICneg	Sample source:Bone marrow serum | Outcome:Long-term responders	RAW_FILE_NAME(File name)=160_0005_01A_R_245SBM3LTRxHILICneg_30AUG24_WAT_WAT_LUM_0000_18AB_15MIN_KP_KP_01.raw
SUBJECT_SAMPLE_FACTORS           	245SBM3LTR	245SBM3LTR_HILICpos	Sample source:Bone marrow serum | Outcome:Long-term responders	RAW_FILE_NAME(File name)=160_0005_01A_R_245SBM3LTRxHILICpos_30AUG24_WAT_WAT_LUM_0000_18AB_15MIN_KP_KP_01.raw
SUBJECT_SAMPLE_FACTORS           	245SBM3LTR	245SBM3LTR_RPneg	Sample source:Bone marrow serum | Outcome:Long-term responders	RAW_FILE_NAME(File name)=160_0005_01B_R_245SBM3LTRxRPneg_03SEP24_WAT_WAT_LUM_0000_18AC_15MIN_KP_KP_01.raw
SUBJECT_SAMPLE_FACTORS           	245SBM3LTR	245SBM3LTR_RPpos	Sample source:Bone marrow serum | Outcome:Long-term responders	RAW_FILE_NAME(File name)=160_0005_01B_R_245SBM3LTRxRPpos_03SEP24_WAT_WAT_LUM_0000_18AC_15MIN_KP_KP_01.raw
SUBJECT_SAMPLE_FACTORS           	413SBM4LTR	413SBM4LTR_HILICneg	Sample source:Bone marrow serum | Outcome:Long-term responders	RAW_FILE_NAME(File name)=160_0005_01A_R_413SBM4LTRxHILICneg_30AUG24_WAT_WAT_LUM_0000_18AB_15MIN_KP_KP_01.raw
SUBJECT_SAMPLE_FACTORS           	413SBM4LTR	413SBM4LTR_HILICpos	Sample source:Bone marrow serum | Outcome:Long-term responders	RAW_FILE_NAME(File name)=160_0005_01A_R_413SBM4LTRxHILICpos_30AUG24_WAT_WAT_LUM_0000_18AB_15MIN_KP_KP_01.raw
SUBJECT_SAMPLE_FACTORS           	413SBM4LTR	413SBM4LTR_RPneg	Sample source:Bone marrow serum | Outcome:Long-term responders	RAW_FILE_NAME(File name)=160_0005_01B_R_413SBM4LTRxRPneg_03SEP24_WAT_WAT_LUM_0000_18AC_15MIN_KP_KP_01.raw
SUBJECT_SAMPLE_FACTORS           	413SBM4LTR	413SBM4LTR_RPpos	Sample source:Bone marrow serum | Outcome:Long-term responders	RAW_FILE_NAME(File name)=160_0005_01B_R_413SBM4LTRxRPpos_03SEP24_WAT_WAT_LUM_0000_18AC_15MIN_KP_KP_01.raw
SUBJECT_SAMPLE_FACTORS           	495SBM5LTR	495SBM5LTR_HILICneg	Sample source:Bone marrow serum | Outcome:Long-term responders	RAW_FILE_NAME(File name)=160_0005_01A_R_495SBM5LTRxHILICneg_30AUG24_WAT_WAT_LUM_0000_18AB_15MIN_KP_KP_01.raw
SUBJECT_SAMPLE_FACTORS           	495SBM5LTR	495SBM5LTR_HILICpos	Sample source:Bone marrow serum | Outcome:Long-term responders	RAW_FILE_NAME(File name)=160_0005_01A_R_495SBM5LTRxHILICpos_30AUG24_WAT_WAT_LUM_0000_18AB_15MIN_KP_KP_02.raw
SUBJECT_SAMPLE_FACTORS           	495SBM5LTR	495SBM5LTR_RPneg	Sample source:Bone marrow serum | Outcome:Long-term responders	RAW_FILE_NAME(File name)=160_0005_01B_R_495SBM5LTRxRPneg_03SEP24_WAT_WAT_LUM_0000_18AC_15MIN_KP_KP_01.raw
SUBJECT_SAMPLE_FACTORS           	495SBM5LTR	495SBM5LTR_RPpos	Sample source:Bone marrow serum | Outcome:Long-term responders	RAW_FILE_NAME(File name)=160_0005_01B_R_495SBM5LTRxRPpos_03SEP24_WAT_WAT_LUM_0000_18AC_15MIN_KP_KP_01.raw
SUBJECT_SAMPLE_FACTORS           	505SBM6LTR	505SBM6LTR_HILICneg	Sample source:Bone marrow serum | Outcome:Long-term responders	RAW_FILE_NAME(File name)=160_0005_01A_R_505SBM6LTRxHILICneg_30AUG24_WAT_WAT_LUM_0000_18AB_15MIN_KP_KP_01.raw
SUBJECT_SAMPLE_FACTORS           	505SBM6LTR	505SBM6LTR_HILICpos	Sample source:Bone marrow serum | Outcome:Long-term responders	RAW_FILE_NAME(File name)=160_0005_01A_R_505SBM6LTRxHILICpos_30AUG24_WAT_WAT_LUM_0000_18AB_15MIN_KP_KP_01.raw
SUBJECT_SAMPLE_FACTORS           	505SBM6LTR	505SBM6LTR_RPneg	Sample source:Bone marrow serum | Outcome:Long-term responders	RAW_FILE_NAME(File name)=160_0005_01B_R_505SBM6LTRxRPneg_03SEP24_WAT_WAT_LUM_0000_18AC_15MIN_KP_KP_01.raw
SUBJECT_SAMPLE_FACTORS           	505SBM6LTR	505SBM6LTR_RPpos	Sample source:Bone marrow serum | Outcome:Long-term responders	RAW_FILE_NAME(File name)=160_0005_01B_R_505SBM6LTRxRPpos_03SEP24_WAT_WAT_LUM_0000_18AC_15MIN_KP_KP_01.raw
SUBJECT_SAMPLE_FACTORS           	518SBM7LTR	518SBM7LTR_HILICneg	Sample source:Bone marrow serum | Outcome:Long-term responders	RAW_FILE_NAME(File name)=160_0005_01A_R_518SBM7LTRxHILICneg_30AUG24_WAT_WAT_LUM_0000_18AB_15MIN_KP_KP_01.raw
SUBJECT_SAMPLE_FACTORS           	518SBM7LTR	518SBM7LTR_HILICpos	Sample source:Bone marrow serum | Outcome:Long-term responders	RAW_FILE_NAME(File name)=160_0005_01A_R_518SBM7LTRxHILICpos_30AUG24_WAT_WAT_LUM_0000_18AB_15MIN_KP_KP_02.raw
SUBJECT_SAMPLE_FACTORS           	518SBM7LTR	518SBM7LTR_RPneg	Sample source:Bone marrow serum | Outcome:Long-term responders	RAW_FILE_NAME(File name)=160_0005_01B_R_518SBM7LTRxRPneg_03SEP24_WAT_WAT_LUM_0000_18AC_15MIN_KP_KP_01.raw
SUBJECT_SAMPLE_FACTORS           	518SBM7LTR	518SBM7LTR_RPpos	Sample source:Bone marrow serum | Outcome:Long-term responders	RAW_FILE_NAME(File name)=160_0005_01B_R_518SBM7LTRxRPpos_03SEP24_WAT_WAT_LUM_0000_18AC_15MIN_KP_KP_01.raw
SUBJECT_SAMPLE_FACTORS           	544SBM8LTR	544SBM8LTR_HILICneg	Sample source:Bone marrow serum | Outcome:Long-term responders	RAW_FILE_NAME(File name)=160_0005_01A_R_544SBM8LTRxHILICneg_30AUG24_WAT_WAT_LUM_0000_18AB_15MIN_KP_KP_01.raw
SUBJECT_SAMPLE_FACTORS           	544SBM8LTR	544SBM8LTR_HILICpos	Sample source:Bone marrow serum | Outcome:Long-term responders	RAW_FILE_NAME(File name)=160_0005_01A_R_544SBM8LTRxHILICpos_30AUG24_WAT_WAT_LUM_0000_18AB_15MIN_KP_KP_01.raw
SUBJECT_SAMPLE_FACTORS           	544SBM8LTR	544SBM8LTR_RPneg	Sample source:Bone marrow serum | Outcome:Long-term responders	RAW_FILE_NAME(File name)=160_0005_01B_R_544SBM8LTRxRPneg_03SEP24_WAT_WAT_LUM_0000_18AC_15MIN_KP_KP_01.raw
SUBJECT_SAMPLE_FACTORS           	544SBM8LTR	544SBM8LTR_RPpos	Sample source:Bone marrow serum | Outcome:Long-term responders	RAW_FILE_NAME(File name)=160_0005_01B_R_544SBM8LTRxRPpos_03SEP24_WAT_WAT_LUM_0000_18AC_15MIN_KP_KP_01.raw
SUBJECT_SAMPLE_FACTORS           	552SBM1LTR	552SBM1LTR_HILICneg	Sample source:Bone marrow serum | Outcome:Long-term responders	RAW_FILE_NAME(File name)=160_0005_01A_R_552SBM1LTRxHILICneg_30AUG24_WAT_WAT_LUM_0000_18AB_15MIN_KP_KP_01.raw
SUBJECT_SAMPLE_FACTORS           	552SBM1LTR	552SBM1LTR_HILICpos	Sample source:Bone marrow serum | Outcome:Long-term responders	RAW_FILE_NAME(File name)=160_0005_01A_R_552SBM1LTRxHILICpos_30AUG24_WAT_WAT_LUM_0000_18AB_15MIN_KP_KP_01.raw
SUBJECT_SAMPLE_FACTORS           	552SBM1LTR	552SBM1LTR_RPneg	Sample source:Bone marrow serum | Outcome:Long-term responders	RAW_FILE_NAME(File name)=160_0005_01B_R_552SBM1LTRxRPneg_03SEP24_WAT_WAT_LUM_0000_18AC_15MIN_KP_KP_01.raw
SUBJECT_SAMPLE_FACTORS           	552SBM1LTR	552SBM1LTR_RPpos	Sample source:Bone marrow serum | Outcome:Long-term responders	RAW_FILE_NAME(File name)=160_0005_01B_R_552SBM1LTRxRPpos_03SEP24_WAT_WAT_LUM_0000_18AC_15MIN_KP_KP_01.raw
SUBJECT_SAMPLE_FACTORS           	209SBM10STR	209SBM10STR_HILICneg	Sample source:Bone marrow serum | Outcome:Short term responders	RAW_FILE_NAME(File name)=160_0005_01A_R_209SBM10STRxHILICneg_30AUG24_WAT_WAT_LUM_0000_18AB_15MIN_KP_KP_01.raw
SUBJECT_SAMPLE_FACTORS           	209SBM10STR	209SBM10STR_HILICpos	Sample source:Bone marrow serum | Outcome:Short term responders	RAW_FILE_NAME(File name)=160_0005_01A_R_209SBM10STRxHILICpos_30AUG24_WAT_WAT_LUM_0000_18AB_15MIN_KP_KP_01.raw
SUBJECT_SAMPLE_FACTORS           	209SBM10STR	209SBM10STR_RPneg	Sample source:Bone marrow serum | Outcome:Short term responders	RAW_FILE_NAME(File name)=160_0005_01B_R_209SBM10STRxRPneg_03SEP24_WAT_WAT_LUM_0000_18AC_15MIN_KP_KP_01.raw
SUBJECT_SAMPLE_FACTORS           	209SBM10STR	209SBM10STR_RPpos	Sample source:Bone marrow serum | Outcome:Short term responders	RAW_FILE_NAME(File name)=160_0005_01B_R_209SBM10STRxRPpos_03SEP24_WAT_WAT_LUM_0000_18AC_15MIN_KP_KP_01.raw
SUBJECT_SAMPLE_FACTORS           	293SBM9STR	293SBM9STR_HILICneg	Sample source:Bone marrow serum | Outcome:Short term responders	RAW_FILE_NAME(File name)=160_0005_01A_R_293SBM9STRxHILICneg_30AUG24_WAT_WAT_LUM_0000_18AB_15MIN_KP_KP_01.raw
SUBJECT_SAMPLE_FACTORS           	293SBM9STR	293SBM9STR_HILICpos	Sample source:Bone marrow serum | Outcome:Short term responders	RAW_FILE_NAME(File name)=160_0005_01A_R_293SBM9STRxHILICpos_30AUG24_WAT_WAT_LUM_0000_18AB_15MIN_KP_KP_01.raw
SUBJECT_SAMPLE_FACTORS           	293SBM9STR	293SBM9STR_RPneg	Sample source:Bone marrow serum | Outcome:Short term responders	RAW_FILE_NAME(File name)=160_0005_01B_R_293SBM9STRxRPneg_03SEP24_WAT_WAT_LUM_0000_18AC_15MIN_KP_KP_01.raw
SUBJECT_SAMPLE_FACTORS           	293SBM9STR	293SBM9STR_RPpos	Sample source:Bone marrow serum | Outcome:Short term responders	RAW_FILE_NAME(File name)=160_0005_01B_R_293SBM9STRxRPpos_03SEP24_WAT_WAT_LUM_0000_18AC_15MIN_KP_KP_01.raw
SUBJECT_SAMPLE_FACTORS           	379SBM11STR	379SBM11STR_HILICneg	Sample source:Bone marrow serum | Outcome:Short term responders	RAW_FILE_NAME(File name)=160_0005_01A_R_379SBM11STRxHILICneg_30AUG24_WAT_WAT_LUM_0000_18AB_15MIN_KP_KP_01.raw
SUBJECT_SAMPLE_FACTORS           	379SBM11STR	379SBM11STR_HILICpos	Sample source:Bone marrow serum | Outcome:Short term responders	RAW_FILE_NAME(File name)=160_0005_01A_R_379SBM11STRxHILICpos_30AUG24_WAT_WAT_LUM_0000_18AB_15MIN_KP_KP_01.raw
SUBJECT_SAMPLE_FACTORS           	379SBM11STR	379SBM11STR_RPneg	Sample source:Bone marrow serum | Outcome:Short term responders	RAW_FILE_NAME(File name)=160_0005_01B_R_379SBM11STRxRPneg_03SEP24_WAT_WAT_LUM_0000_18AC_15MIN_KP_KP_01.raw
SUBJECT_SAMPLE_FACTORS           	379SBM11STR	379SBM11STR_RPpos	Sample source:Bone marrow serum | Outcome:Short term responders	RAW_FILE_NAME(File name)=160_0005_01B_R_379SBM11STRxRPpos_03SEP24_WAT_WAT_LUM_0000_18AC_15MIN_KP_KP_01.raw
SUBJECT_SAMPLE_FACTORS           	397SBM12STR	397SBM12STR_HILICneg	Sample source:Bone marrow serum | Outcome:Short term responders	RAW_FILE_NAME(File name)=160_0005_01A_R_397SBM12STRxHILICneg_30AUG24_WAT_WAT_LUM_0000_18AB_15MIN_KP_KP_01.raw
SUBJECT_SAMPLE_FACTORS           	397SBM12STR	397SBM12STR_HILICpos	Sample source:Bone marrow serum | Outcome:Short term responders	RAW_FILE_NAME(File name)=160_0005_01A_R_397SBM12STRxHILICpos_30AUG24_WAT_WAT_LUM_0000_18AB_15MIN_KP_KP_01.raw
SUBJECT_SAMPLE_FACTORS           	397SBM12STR	397SBM12STR_RPneg	Sample source:Bone marrow serum | Outcome:Short term responders	RAW_FILE_NAME(File name)=160_0005_01B_R_397SBM12STRxRPneg_03SEP24_WAT_WAT_LUM_0000_18AC_15MIN_KP_KP_01.raw
SUBJECT_SAMPLE_FACTORS           	397SBM12STR	397SBM12STR_RPpos	Sample source:Bone marrow serum | Outcome:Short term responders	RAW_FILE_NAME(File name)=160_0005_01B_R_397SBM12STRxRPpos_03SEP24_WAT_WAT_LUM_0000_18AC_15MIN_KP_KP_01.raw
SUBJECT_SAMPLE_FACTORS           	431SBM13STR	431SBM13STR_HILICneg	Sample source:Bone marrow serum | Outcome:Short term responders	RAW_FILE_NAME(File name)=160_0005_01A_R_431SBM13STRxHILICneg_30AUG24_WAT_WAT_LUM_0000_18AB_15MIN_KP_KP_01.raw
SUBJECT_SAMPLE_FACTORS           	431SBM13STR	431SBM13STR_HILICpos	Sample source:Bone marrow serum | Outcome:Short term responders	RAW_FILE_NAME(File name)=160_0005_01A_R_431SBM13STRxHILICpos_30AUG24_WAT_WAT_LUM_0000_18AB_15MIN_KP_KP_02.raw
SUBJECT_SAMPLE_FACTORS           	431SBM13STR	431SBM13STR_RPneg	Sample source:Bone marrow serum | Outcome:Short term responders	RAW_FILE_NAME(File name)=160_0005_01B_R_431SBM13STRxRPneg_03SEP24_WAT_WAT_LUM_0000_18AC_15MIN_KP_KP_01.raw
SUBJECT_SAMPLE_FACTORS           	431SBM13STR	431SBM13STR_RPpos	Sample source:Bone marrow serum | Outcome:Short term responders	RAW_FILE_NAME(File name)=160_0005_01B_R_431SBM13STRxRPpos_03SEP24_WAT_WAT_LUM_0000_18AC_15MIN_KP_KP_01.raw
SUBJECT_SAMPLE_FACTORS           	466SBM14STR	466SBM14STR_HILICneg	Sample source:Bone marrow serum | Outcome:Short term responders	RAW_FILE_NAME(File name)=160_0005_01A_R_466SBM14STRxHILICneg_30AUG24_WAT_WAT_LUM_0000_18AB_15MIN_KP_KP_01.raw
SUBJECT_SAMPLE_FACTORS           	466SBM14STR	466SBM14STR_HILICpos	Sample source:Bone marrow serum | Outcome:Short term responders	RAW_FILE_NAME(File name)=160_0005_01A_R_466SBM14STRxHILICpos_30AUG24_WAT_WAT_LUM_0000_18AB_15MIN_KP_KP_01.raw
SUBJECT_SAMPLE_FACTORS           	466SBM14STR	466SBM14STR_RPneg	Sample source:Bone marrow serum | Outcome:Short term responders	RAW_FILE_NAME(File name)=160_0005_01B_R_466SBM14STRxRPneg_03SEP24_WAT_WAT_LUM_0000_18AC_15MIN_KP_KP_01.raw
SUBJECT_SAMPLE_FACTORS           	466SBM14STR	466SBM14STR_RPpos	Sample source:Bone marrow serum | Outcome:Short term responders	RAW_FILE_NAME(File name)=160_0005_01B_R_466SBM14STRxRPpos_03SEP24_WAT_WAT_LUM_0000_18AC_15MIN_KP_KP_01.raw
SUBJECT_SAMPLE_FACTORS           	543SBM15STR	543SBM15STR_HILICneg	Sample source:Bone marrow serum | Outcome:Short term responders	RAW_FILE_NAME(File name)=160_0005_01A_R_543SBM15STRxHILICneg_30AUG24_WAT_WAT_LUM_0000_18AB_15MIN_KP_KP_01.raw
SUBJECT_SAMPLE_FACTORS           	543SBM15STR	543SBM15STR_HILICpos	Sample source:Bone marrow serum | Outcome:Short term responders	RAW_FILE_NAME(File name)=160_0005_01A_R_543SBM15STRxHILICpos_30AUG24_WAT_WAT_LUM_0000_18AB_15MIN_KP_KP_02.raw
SUBJECT_SAMPLE_FACTORS           	543SBM15STR	543SBM15STR_RPneg	Sample source:Bone marrow serum | Outcome:Short term responders	RAW_FILE_NAME(File name)=160_0005_01B_R_543SBM15STRxRPneg_03SEP24_WAT_WAT_LUM_0000_18AC_15MIN_KP_KP_01.raw
SUBJECT_SAMPLE_FACTORS           	543SBM15STR	543SBM15STR_RPpos	Sample source:Bone marrow serum | Outcome:Short term responders	RAW_FILE_NAME(File name)=160_0005_01B_R_543SBM15STRxRPpos_03SEP24_WAT_WAT_LUM_0000_18AC_15MIN_KP_KP_01.raw
SUBJECT_SAMPLE_FACTORS           	549SBM16STR	549SBM16STR_HILICneg	Sample source:Bone marrow serum | Outcome:Short term responders	RAW_FILE_NAME(File name)=160_0005_01A_R_549SBM16STRxHILICneg_30AUG24_WAT_WAT_LUM_0000_18AB_15MIN_KP_KP_01.raw
SUBJECT_SAMPLE_FACTORS           	549SBM16STR	549SBM16STR_HILICpos	Sample source:Bone marrow serum | Outcome:Short term responders	RAW_FILE_NAME(File name)=160_0005_01A_R_549SBM16STRxHILICpos_30AUG24_WAT_WAT_LUM_0000_18AB_15MIN_KP_KP_01.raw
SUBJECT_SAMPLE_FACTORS           	549SBM16STR	549SBM16STR_RPneg	Sample source:Bone marrow serum | Outcome:Short term responders	RAW_FILE_NAME(File name)=160_0005_01B_R_549SBM16STRxRPneg_03SEP24_WAT_WAT_LUM_0000_18AC_15MIN_KP_KP_01.raw
SUBJECT_SAMPLE_FACTORS           	549SBM16STR	549SBM16STR_RPpos	Sample source:Bone marrow serum | Outcome:Short term responders	RAW_FILE_NAME(File name)=160_0005_01B_R_549SBM16STRxRPpos_03SEP24_WAT_WAT_LUM_0000_18AC_15MIN_KP_KP_01.raw
#COLLECTION
CO:COLLECTION_SUMMARY            	Bone marrow serum (post-infusion CAR T cells in bone marrow) was collected 28day
CO:COLLECTION_SUMMARY            	post CAR-T cell infusion in leukemia patients
CO:SAMPLE_TYPE                   	Serum of bone marrow
CO:STORAGE_CONDITIONS            	-80℃
#TREATMENT
TR:TREATMENT_SUMMARY             	The patients were treated with CAR-T cells and after 28th day post infusion they
TR:TREATMENT_SUMMARY             	achieved the complete remission.
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	For bonemarrow, 3-fold excess acetonitrile: methanol (3:1) was added for protein
SP:SAMPLEPREP_SUMMARY            	removal. The resultant metabolite extracts from bone marrow were recovered by
SP:SAMPLEPREP_SUMMARY            	centrifuging at 13,226 xg at 4°C for 15 min. For HILIC chromatography, the
SP:SAMPLEPREP_SUMMARY            	extract was directly used LC-MS analysis. For reversed phase chromatography, the
SP:SAMPLEPREP_SUMMARY            	extract was dried under vacuum centrifugation and the dried extract was
SP:SAMPLEPREP_SUMMARY            	reconstituted in 20% Acetonitrile, 80% water and used used for LC-MS analysis.
SP:PROCESSING_STORAGE_CONDITIONS 	-80℃
SP:EXTRACT_STORAGE               	-80℃
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_SUMMARY        	HILIC
CH:INSTRUMENT_NAME               	Thermo Vanquish
CH:COLUMN_NAME                   	Waters ACQUITY UPLC BEH Amide (150 x 2.1mm,1.7um)
CH:COLUMN_TEMPERATURE            	45
CH:FLOW_GRADIENT                 	99% B for 1 min, 99–85% B for 3 min, 85–75% B for 3 min, 75–30% B
CH:FLOW_GRADIENT                 	for 3 min, 30% B for 1 min, 30-99%B for 1 min and 99%B for 4 min
CH:FLOW_RATE                     	0.35 ml/min
CH:SOLVENT_A                     	95% water/5% acetonitrile; 10mM ammonium acetate; 0.1% acetic acid
CH:SOLVENT_B                     	95% acetonitrilie/5% water; 10mM ammonium acetate; 0.1% acetic acid
CH:CHROMATOGRAPHY_TYPE           	HILIC
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
#MS
MS:INSTRUMENT_NAME               	Thermo Orbitrap Fusion Lumos Tribrid
MS:INSTRUMENT_TYPE               	Orbitrap
MS:MS_TYPE                       	ESI
MS:MS_COMMENTS                   	Xcalibur Compound Discoverer 3.2
MS:ION_MODE                      	NEGATIVE
MS:MS_RESULTS_FILE               	ST003966_AN006524_Results.txt	UNITS:Peak area	Has m/z:Yes	Has RT:Yes	RT units:Minutes
#END