#METABOLOMICS WORKBENCH cdb9006_20250608_085913 DATATRACK_ID:6012 STUDY_ID:ST003969 ANALYSIS_ID:AN006530 PROJECT_ID:PR002485
VERSION             	1
CREATED_ON             	June 13, 2025, 5:02 am
#PROJECT
PR:PROJECT_TITLE                 	Effect of Desiccation of Mycobacterium tuberculosis metabolism
PR:PROJECT_SUMMARY               	The goal of this project was to measure metabolite shifts in Mycobacterium
PR:PROJECT_SUMMARY               	tuberculosis (Mtb) in response to desiccation and rehydration. We characterized
PR:PROJECT_SUMMARY               	the metabolome of Mtb in log phase and compared it to cells that were either
PR:PROJECT_SUMMARY               	dried for 24 hours or held in a solution of 200 mM sodium chloride. Following
PR:PROJECT_SUMMARY               	this incubation cells were rehydrated into 200 mM sodium chloride. We performed
PR:PROJECT_SUMMARY               	targeted metabolomics on each of these conditions using LC-MS. Significant
PR:PROJECT_SUMMARY               	increases in oxidized nucleotides and intermediates of de novo nucleotide
PR:PROJECT_SUMMARY               	biosynthesis were discovered in the desiccated samples. These metaboite
PR:PROJECT_SUMMARY               	accumulations reversed with rehydration.
PR:INSTITUTE                     	Weill Cornell Medicine
PR:DEPARTMENT                    	Department of Medicine, Division of Infectious Diseases
PR:LABORATORY                    	Rhee Lab
PR:LAST_NAME                     	Brown
PR:FIRST_NAME                    	Christopher
PR:ADDRESS                       	413 E 69th St, New York, NY, 10021, USA
PR:EMAIL                         	cdb9006@med.cornell.edu
PR:PHONE                         	6469626225
PR:FUNDING_SOURCE                	P01AI159402 (PI: Rhee)
PR:PUBLICATIONS                  	https://doi.org/10.1101/2025.06.04.657859
#STUDY
ST:STUDY_TITLE                   	Effect of Desiccation of Mycobacterium tuberculosis metabolism
ST:STUDY_TYPE                    	Metabolomics
ST:STUDY_SUMMARY                 	Mycobacterium tuberculosis (Mtb) is an obligate human pathogen that depends on
ST:STUDY_SUMMARY                 	its ability to spread from host-to-host to survive as a species. Yet, knowledge
ST:STUDY_SUMMARY                 	of transmission-specific traits remains lacking. Here, we report the discovery
ST:STUDY_SUMMARY                 	of a specific adaptive response to desiccation, a stress intrinsically linked to
ST:STUDY_SUMMARY                 	the generation of the aerosol droplets within which Mtb transmits. We show that
ST:STUDY_SUMMARY                 	desiccation inflicts oxidative damage and activates Mtb’s DNA repair responses
ST:STUDY_SUMMARY                 	but that this repair is imperfect and results in mutations. We further show that
ST:STUDY_SUMMARY                 	activation of these DNA repair responses is accompanied by increased expression
ST:STUDY_SUMMARY                 	of the transcription-coupled repair factor, mfd, but that this expression serves
ST:STUDY_SUMMARY                 	to buffer the fitness cost of specific resistance-conferring mutations in rpoB,
ST:STUDY_SUMMARY                 	the target of the frontline drug rifampin, rather than to facilitate
ST:STUDY_SUMMARY                 	transcription-coupled DNA repair. Silencing mfd during aerosolization impairs
ST:STUDY_SUMMARY                 	survival of strains harboring the rifampin resistance allele S450L. This
ST:STUDY_SUMMARY                 	function is further supported by whole genome sequence data from over 50,000
ST:STUDY_SUMMARY                 	clinically circulating strains. These studies indicate that Mtb has evolved
ST:STUDY_SUMMARY                 	transmission-specific stress responses that have enabled it to leverage
ST:STUDY_SUMMARY                 	desiccation-induced DNA damage as a potential source of genetic diversification
ST:STUDY_SUMMARY                 	and drug resistance.
ST:INSTITUTE                     	Weill Cornell Medicine
ST:DEPARTMENT                    	Department of Medicine, Division of Infectious Diseases
ST:LABORATORY                    	Rhee Lab
ST:LAST_NAME                     	Brown
ST:FIRST_NAME                    	Christopher
ST:ADDRESS                       	413 E 69th St
ST:EMAIL                         	cdb9006@med.cornell.edu
ST:PHONE                         	16469626225
ST:NUM_GROUPS                    	5
ST:PUBLICATIONS                  	https://doi.org/10.1101/2025.06.04.657859
#SUBJECT
SU:SUBJECT_TYPE                  	Bacteria
SU:SUBJECT_SPECIES               	Mycobacterium tuberculosis (H37Rv)
SU:TAXONOMY_ID                   	83332
SU:GENOTYPE_STRAIN               	H37Rv
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data
SUBJECT_SAMPLE_FACTORS           	-	20220329_RNAseq_D01_FA_March22_Neg	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:Log phase biofilm growth on 7H10	Chromatography=Normal phase formic acid; Mode=Negative; Experimental replicate=Experiment 1; RAW_FILE_NAME(Raw data file names)=20220329_RNAseq_D01_FA_March22_Neg.d; mzXML file names=20220329_RNAseq_D01_FA_March22_Neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	20220329_RNAseq_D02_FA_March22_Neg	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:Log phase biofilm growth on 7H10	Chromatography=Normal phase formic acid; Mode=Negative; Experimental replicate=Experiment 1; RAW_FILE_NAME(Raw data file names)=20220329_RNAseq_D02_FA_March22_Neg.d; mzXML file names=20220329_RNAseq_D02_FA_March22_Neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	20220329_RNAseq_D03_FA_March22_Neg	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:Log phase biofilm growth on 7H10	Chromatography=Normal phase formic acid; Mode=Negative; Experimental replicate=Experiment 1; RAW_FILE_NAME(Raw data file names)=20220329_RNAseq_D03_FA_March22_Neg.d; mzXML file names=20220329_RNAseq_D03_FA_March22_Neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	20220329_RNAseq_D11_FA_March22_Neg	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:24 hours of desiccation from biofilm	Chromatography=Normal phase formic acid; Mode=Negative; Experimental replicate=Experiment 1; RAW_FILE_NAME(Raw data file names)=20220329_RNAseq_D11_FA_March22_Neg.d; mzXML file names=20220329_RNAseq_D11_FA_March22_Neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	20220329_RNAseq_D12_FA_March22_Neg	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:24 hours of desiccation from biofilm	Chromatography=Normal phase formic acid; Mode=Negative; Experimental replicate=Experiment 1; RAW_FILE_NAME(Raw data file names)=20220329_RNAseq_D12_FA_March22_Neg.d; mzXML file names=20220329_RNAseq_D12_FA_March22_Neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	20220329_RNAseq_D13_FA_March22_Neg	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:24 hours of desiccation from biofilm	Chromatography=Normal phase formic acid; Mode=Negative; Experimental replicate=Experiment 1; RAW_FILE_NAME(Raw data file names)=20220329_RNAseq_D13_FA_March22_Neg.d; mzXML file names=20220329_RNAseq_D13_FA_March22_Neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	20220329_RNAseq_RD1_FA_March22_Neg	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:Rehydration into 200 mM NaCl after 24 hours of desiccation	Chromatography=Normal phase formic acid; Mode=Negative; Experimental replicate=Experiment 1; RAW_FILE_NAME(Raw data file names)=20220329_RNAseq_RD1_FA_March22_Neg.d; mzXML file names=20220329_RNAseq_RD1_FA_March22_Neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	20220329_RNAseq_RD2_FA_March22_Neg	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:Rehydration into 200 mM NaCl after 24 hours of desiccation	Chromatography=Normal phase formic acid; Mode=Negative; Experimental replicate=Experiment 1; RAW_FILE_NAME(Raw data file names)=20220329_RNAseq_RD2_FA_March22_Neg.d; mzXML file names=20220329_RNAseq_RD2_FA_March22_Neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	20220329_RNAseq_RD3_FA_March22_Neg	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:Rehydration into 200 mM NaCl after 24 hours of desiccation	Chromatography=Normal phase formic acid; Mode=Negative; Experimental replicate=Experiment 1; RAW_FILE_NAME(Raw data file names)=20220329_RNAseq_RD3_FA_March22_Neg.d; mzXML file names=20220329_RNAseq_RD3_FA_March22_Neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	20220329_RNAseq_RW1_FA_March22_Neg	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:Rehydration into 200 mM NaCl after 24 hours in 200 mM NaCl	Chromatography=Normal phase formic acid; Mode=Negative; Experimental replicate=Experiment 1; RAW_FILE_NAME(Raw data file names)=20220329_RNAseq_RW1_FA_March22_Neg.d; mzXML file names=20220329_RNAseq_RW1_FA_March22_Neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	20220329_RNAseq_RW2_FA_March22_Neg	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:Rehydration into 200 mM NaCl after 24 hours in 200 mM NaCl	Chromatography=Normal phase formic acid; Mode=Negative; Experimental replicate=Experiment 1; RAW_FILE_NAME(Raw data file names)=20220329_RNAseq_RW2_FA_March22_Neg.d; mzXML file names=20220329_RNAseq_RW2_FA_March22_Neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	20220329_RNAseq_RW3_FA_March22_Neg	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:Rehydration into 200 mM NaCl after 24 hours in 200 mM NaCl	Chromatography=Normal phase formic acid; Mode=Negative; Experimental replicate=Experiment 1; RAW_FILE_NAME(Raw data file names)=20220329_RNAseq_RW3_FA_March22_Neg.d; mzXML file names=20220329_RNAseq_RW3_FA_March22_Neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	20220329_RNAseq_W11_FA_March22_Neg	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:24 hours of incubation in 200 mM NaCl	Chromatography=Normal phase formic acid; Mode=Negative; Experimental replicate=Experiment 1; RAW_FILE_NAME(Raw data file names)=20220329_RNAseq_W11_FA_March22_Neg.d; mzXML file names=20220329_RNAseq_W11_FA_March22_Neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	20220329_RNAseq_W12_FA_March22_Neg	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:24 hours of incubation in 200 mM NaCl	Chromatography=Normal phase formic acid; Mode=Negative; Experimental replicate=Experiment 1; RAW_FILE_NAME(Raw data file names)=20220329_RNAseq_W12_FA_March22_Neg.d; mzXML file names=20220329_RNAseq_W12_FA_March22_Neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	20220329_RNAseq_W13_FA_March22_Neg	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:24 hours of incubation in 200 mM NaCl	Chromatography=Normal phase formic acid; Mode=Negative; Experimental replicate=Experiment 1; RAW_FILE_NAME(Raw data file names)=20220329_RNAseq_W13_FA_March22_Neg.d; mzXML file names=20220329_RNAseq_W13_FA_March22_Neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	051823_NEG_RH70_D01	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:Log phase biofilm growth on 7H10	Chromatography=Normal phase formic acid; Mode=Negative; Experimental replicate=Experiment 2; RAW_FILE_NAME(Raw data file names)=051823_NEG_RH70_D01.d; mzXML file names=051823_NEG_RH70_D01.mzXML
SUBJECT_SAMPLE_FACTORS           	-	051823_NEG_RH70_D02	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:Log phase biofilm growth on 7H10	Chromatography=Normal phase formic acid; Mode=Negative; Experimental replicate=Experiment 2; RAW_FILE_NAME(Raw data file names)=051823_NEG_RH70_D02.d; mzXML file names=051823_NEG_RH70_D02.mzXML
SUBJECT_SAMPLE_FACTORS           	-	051823_NEG_RH70_D03	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:Log phase biofilm growth on 7H10	Chromatography=Normal phase formic acid; Mode=Negative; Experimental replicate=Experiment 2; RAW_FILE_NAME(Raw data file names)=051823_NEG_RH70_D03.d; mzXML file names=051823_NEG_RH70_D03.mzXML
SUBJECT_SAMPLE_FACTORS           	-	051823_NEG_RH70_D11	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:24 hours of desiccation from biofilm	Chromatography=Normal phase formic acid; Mode=Negative; Experimental replicate=Experiment 2; RAW_FILE_NAME(Raw data file names)=051823_NEG_RH70_D11.d; mzXML file names=051823_NEG_RH70_D11.mzXML
SUBJECT_SAMPLE_FACTORS           	-	051823_NEG_RH70_D12	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:24 hours of desiccation from biofilm	Chromatography=Normal phase formic acid; Mode=Negative; Experimental replicate=Experiment 2; RAW_FILE_NAME(Raw data file names)=051823_NEG_RH70_D12.d; mzXML file names=051823_NEG_RH70_D12.mzXML
SUBJECT_SAMPLE_FACTORS           	-	051823_NEG_RH70_D13	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:24 hours of desiccation from biofilm	Chromatography=Normal phase formic acid; Mode=Negative; Experimental replicate=Experiment 2; RAW_FILE_NAME(Raw data file names)=051823_NEG_RH70_D13.d; mzXML file names=051823_NEG_RH70_D13.mzXML
SUBJECT_SAMPLE_FACTORS           	-	051823_NEG_RH70_RD1	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:Rehydration into 200 mM NaCl after 24 hours of desiccation	Chromatography=Normal phase formic acid; Mode=Negative; Experimental replicate=Experiment 2; RAW_FILE_NAME(Raw data file names)=051823_NEG_RH70_RD1.d; mzXML file names=051823_NEG_RH70_RD1.mzXML
SUBJECT_SAMPLE_FACTORS           	-	051823_NEG_RH70_RD2	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:Rehydration into 200 mM NaCl after 24 hours of desiccation	Chromatography=Normal phase formic acid; Mode=Negative; Experimental replicate=Experiment 2; RAW_FILE_NAME(Raw data file names)=051823_NEG_RH70_RD2.d; mzXML file names=051823_NEG_RH70_RD2.mzXML
SUBJECT_SAMPLE_FACTORS           	-	051823_NEG_RH70_RD3	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:Rehydration into 200 mM NaCl after 24 hours of desiccation	Chromatography=Normal phase formic acid; Mode=Negative; Experimental replicate=Experiment 2; RAW_FILE_NAME(Raw data file names)=051823_NEG_RH70_RD3.d; mzXML file names=051823_NEG_RH70_RD3.mzXML
SUBJECT_SAMPLE_FACTORS           	-	051823_NEG_RH70_RW1	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:Rehydration into 200 mM NaCl after 24 hours in 200 mM NaCl	Chromatography=Normal phase formic acid; Mode=Negative; Experimental replicate=Experiment 2; RAW_FILE_NAME(Raw data file names)=051823_NEG_RH70_RW1.d; mzXML file names=051823_NEG_RH70_RW1.mzXML
SUBJECT_SAMPLE_FACTORS           	-	051823_NEG_RH70_RW2	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:Rehydration into 200 mM NaCl after 24 hours in 200 mM NaCl	Chromatography=Normal phase formic acid; Mode=Negative; Experimental replicate=Experiment 2; RAW_FILE_NAME(Raw data file names)=051823_NEG_RH70_RW2.d; mzXML file names=051823_NEG_RH70_RW2.mzXML
SUBJECT_SAMPLE_FACTORS           	-	051823_NEG_RH70_RW3	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:Rehydration into 200 mM NaCl after 24 hours in 200 mM NaCl	Chromatography=Normal phase formic acid; Mode=Negative; Experimental replicate=Experiment 2; RAW_FILE_NAME(Raw data file names)=051823_NEG_RH70_RW3.d; mzXML file names=051823_NEG_RH70_RW3.mzXML
SUBJECT_SAMPLE_FACTORS           	-	051823_NEG_RH70_W11	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:24 hours of incubation in 200 mM NaCl	Chromatography=Normal phase formic acid; Mode=Negative; Experimental replicate=Experiment 2; RAW_FILE_NAME(Raw data file names)=051823_NEG_RH70_W11.d; mzXML file names=051823_NEG_RH70_W11.mzXML
SUBJECT_SAMPLE_FACTORS           	-	051823_NEG_RH70_W12	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:24 hours of incubation in 200 mM NaCl	Chromatography=Normal phase formic acid; Mode=Negative; Experimental replicate=Experiment 2; RAW_FILE_NAME(Raw data file names)=051823_NEG_RH70_W12.d; mzXML file names=051823_NEG_RH70_W12.mzXML
SUBJECT_SAMPLE_FACTORS           	-	051823_NEG_RH70_W13	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:24 hours of incubation in 200 mM NaCl	Chromatography=Normal phase formic acid; Mode=Negative; Experimental replicate=Experiment 2; RAW_FILE_NAME(Raw data file names)=051823_NEG_RH70_W13.d; mzXML file names=051823_NEG_RH70_W13.mzXML
SUBJECT_SAMPLE_FACTORS           	-	20220329_RNAseq_D01_FA_March22_Pos	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:Log phase biofilm growth on 7H10	Chromatography=Normal phase formic acid; Mode=Positive; Experimental replicate=Experiment 1; RAW_FILE_NAME(Raw data file names)=20220329_RNAseq_D01_FA_March22_Pos.d; mzXML file names=20220329_RNAseq_D01_FA_March22_Pos.mzXML
SUBJECT_SAMPLE_FACTORS           	-	20220329_RNAseq_D02_FA_March22_Pos	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:Log phase biofilm growth on 7H10	Chromatography=Normal phase formic acid; Mode=Positive; Experimental replicate=Experiment 1; RAW_FILE_NAME(Raw data file names)=20220329_RNAseq_D02_FA_March22_Pos.d; mzXML file names=20220329_RNAseq_D02_FA_March22_Pos.mzXML
SUBJECT_SAMPLE_FACTORS           	-	20220329_RNAseq_D03_FA_March22_Pos	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:Log phase biofilm growth on 7H10	Chromatography=Normal phase formic acid; Mode=Positive; Experimental replicate=Experiment 1; RAW_FILE_NAME(Raw data file names)=20220329_RNAseq_D03_FA_March22_Pos.d; mzXML file names=20220329_RNAseq_D03_FA_March22_Pos.mzXML
SUBJECT_SAMPLE_FACTORS           	-	20220329_RNAseq_D11_FA_March22_Pos	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:24 hours of desiccation from biofilm	Chromatography=Normal phase formic acid; Mode=Positive; Experimental replicate=Experiment 1; RAW_FILE_NAME(Raw data file names)=20220329_RNAseq_D11_FA_March22_Pos.d; mzXML file names=20220329_RNAseq_D11_FA_March22_Pos.mzXML
SUBJECT_SAMPLE_FACTORS           	-	20220329_RNAseq_D12_FA_March22_Pos	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:24 hours of desiccation from biofilm	Chromatography=Normal phase formic acid; Mode=Positive; Experimental replicate=Experiment 1; RAW_FILE_NAME(Raw data file names)=20220329_RNAseq_D12_FA_March22_Pos.d; mzXML file names=20220329_RNAseq_D12_FA_March22_Pos.mzXML
SUBJECT_SAMPLE_FACTORS           	-	20220329_RNAseq_D13_FA_March22_Pos	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:24 hours of desiccation from biofilm	Chromatography=Normal phase formic acid; Mode=Positive; Experimental replicate=Experiment 1; RAW_FILE_NAME(Raw data file names)=20220329_RNAseq_D13_FA_March22_Pos.d; mzXML file names=20220329_RNAseq_D13_FA_March22_Pos.mzXML
SUBJECT_SAMPLE_FACTORS           	-	20220329_RNAseq_RD1_FA_March22_Pos	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:Rehydration into 200 mM NaCl after 24 hours of desiccation	Chromatography=Normal phase formic acid; Mode=Positive; Experimental replicate=Experiment 1; RAW_FILE_NAME(Raw data file names)=20220329_RNAseq_RD1_FA_March22_Pos.d; mzXML file names=20220329_RNAseq_RD1_FA_March22_Pos.mzXML
SUBJECT_SAMPLE_FACTORS           	-	20220329_RNAseq_RD2_FA_March22_Pos	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:Rehydration into 200 mM NaCl after 24 hours of desiccation	Chromatography=Normal phase formic acid; Mode=Positive; Experimental replicate=Experiment 1; RAW_FILE_NAME(Raw data file names)=20220329_RNAseq_RD2_FA_March22_Pos.d; mzXML file names=20220329_RNAseq_RD2_FA_March22_Pos.mzXML
SUBJECT_SAMPLE_FACTORS           	-	20220329_RNAseq_RD3_FA_March22_Pos	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:Rehydration into 200 mM NaCl after 24 hours of desiccation	Chromatography=Normal phase formic acid; Mode=Positive; Experimental replicate=Experiment 1; RAW_FILE_NAME(Raw data file names)=20220329_RNAseq_RD3_FA_March22_Pos.d; mzXML file names=20220329_RNAseq_RD3_FA_March22_Pos.mzXML
SUBJECT_SAMPLE_FACTORS           	-	20220329_RNAseq_RW1_FA_March22_Pos	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:Rehydration into 200 mM NaCl after 24 hours in 200 mM NaCl	Chromatography=Normal phase formic acid; Mode=Positive; Experimental replicate=Experiment 1; RAW_FILE_NAME(Raw data file names)=20220329_RNAseq_RW1_FA_March22_Pos.d; mzXML file names=20220329_RNAseq_RW1_FA_March22_Pos.mzXML
SUBJECT_SAMPLE_FACTORS           	-	20220329_RNAseq_RW2_FA_March22_Pos	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:Rehydration into 200 mM NaCl after 24 hours in 200 mM NaCl	Chromatography=Normal phase formic acid; Mode=Positive; Experimental replicate=Experiment 1; RAW_FILE_NAME(Raw data file names)=20220329_RNAseq_RW2_FA_March22_Pos.d; mzXML file names=20220329_RNAseq_RW2_FA_March22_Pos.mzXML
SUBJECT_SAMPLE_FACTORS           	-	20220329_RNAseq_RW3_FA_March22_Pos	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:Rehydration into 200 mM NaCl after 24 hours in 200 mM NaCl	Chromatography=Normal phase formic acid; Mode=Positive; Experimental replicate=Experiment 1; RAW_FILE_NAME(Raw data file names)=20220329_RNAseq_RW3_FA_March22_Pos.d; mzXML file names=20220329_RNAseq_RW3_FA_March22_Pos.mzXML
SUBJECT_SAMPLE_FACTORS           	-	20220329_RNAseq_W11_FA_March22_Pos	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:24 hours of incubation in 200 mM NaCl	Chromatography=Normal phase formic acid; Mode=Positive; Experimental replicate=Experiment 1; RAW_FILE_NAME(Raw data file names)=20220329_RNAseq_W11_FA_March22_Pos.d; mzXML file names=20220329_RNAseq_W11_FA_March22_Pos.mzXML
SUBJECT_SAMPLE_FACTORS           	-	20220329_RNAseq_W12_FA_March22_Pos	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:24 hours of incubation in 200 mM NaCl	Chromatography=Normal phase formic acid; Mode=Positive; Experimental replicate=Experiment 1; RAW_FILE_NAME(Raw data file names)=20220329_RNAseq_W12_FA_March22_Pos.d; mzXML file names=20220329_RNAseq_W12_FA_March22_Pos.mzXML
SUBJECT_SAMPLE_FACTORS           	-	20220329_RNAseq_W13_FA_March22_Pos	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:24 hours of incubation in 200 mM NaCl	Chromatography=Normal phase formic acid; Mode=Positive; Experimental replicate=Experiment 1; RAW_FILE_NAME(Raw data file names)=20220329_RNAseq_W13_FA_March22_Pos.d; mzXML file names=20220329_RNAseq_W13_FA_March22_Pos.mzXML
SUBJECT_SAMPLE_FACTORS           	-	051823_POS_RH70_D01	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:Log phase biofilm growth on 7H10	Chromatography=Normal phase formic acid; Mode=Positive; Experimental replicate=Experiment 2; RAW_FILE_NAME(Raw data file names)=051823_POS_RH70_D01.d; mzXML file names=051823_POS_RH70_D01.mzXML
SUBJECT_SAMPLE_FACTORS           	-	051823_POS_RH70_D02	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:Log phase biofilm growth on 7H10	Chromatography=Normal phase formic acid; Mode=Positive; Experimental replicate=Experiment 2; RAW_FILE_NAME(Raw data file names)=051823_POS_RH70_D02.d; mzXML file names=051823_POS_RH70_D02.mzXML
SUBJECT_SAMPLE_FACTORS           	-	051823_POS_RH70_D03	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:Log phase biofilm growth on 7H10	Chromatography=Normal phase formic acid; Mode=Positive; Experimental replicate=Experiment 2; RAW_FILE_NAME(Raw data file names)=051823_POS_RH70_D03.d; mzXML file names=051823_POS_RH70_D03.mzXML
SUBJECT_SAMPLE_FACTORS           	-	051823_POS_RH70_D11	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:24 hours of desiccation from biofilm	Chromatography=Normal phase formic acid; Mode=Positive; Experimental replicate=Experiment 2; RAW_FILE_NAME(Raw data file names)=051823_POS_RH70_D11.d; mzXML file names=051823_POS_RH70_D11.mzXML
SUBJECT_SAMPLE_FACTORS           	-	051823_POS_RH70_D12	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:24 hours of desiccation from biofilm	Chromatography=Normal phase formic acid; Mode=Positive; Experimental replicate=Experiment 2; RAW_FILE_NAME(Raw data file names)=051823_POS_RH70_D12.d; mzXML file names=051823_POS_RH70_D12.mzXML
SUBJECT_SAMPLE_FACTORS           	-	051823_POS_RH70_D13	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:24 hours of desiccation from biofilm	Chromatography=Normal phase formic acid; Mode=Positive; Experimental replicate=Experiment 2; RAW_FILE_NAME(Raw data file names)=051823_POS_RH70_D13.d; mzXML file names=051823_POS_RH70_D13.mzXML
SUBJECT_SAMPLE_FACTORS           	-	051823_POS_RH70_RD1	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:Rehydration into 200 mM NaCl after 24 hours of desiccation	Chromatography=Normal phase formic acid; Mode=Positive; Experimental replicate=Experiment 2; RAW_FILE_NAME(Raw data file names)=051823_POS_RH70_RD1.d; mzXML file names=051823_POS_RH70_RD1.mzXML
SUBJECT_SAMPLE_FACTORS           	-	051823_POS_RH70_RD2	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:Rehydration into 200 mM NaCl after 24 hours of desiccation	Chromatography=Normal phase formic acid; Mode=Positive; Experimental replicate=Experiment 2; RAW_FILE_NAME(Raw data file names)=051823_POS_RH70_RD2.d; mzXML file names=051823_POS_RH70_RD2.mzXML
SUBJECT_SAMPLE_FACTORS           	-	051823_POS_RH70_RD3	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:Rehydration into 200 mM NaCl after 24 hours of desiccation	Chromatography=Normal phase formic acid; Mode=Positive; Experimental replicate=Experiment 2; RAW_FILE_NAME(Raw data file names)=051823_POS_RH70_RD3.d; mzXML file names=051823_POS_RH70_RD3.mzXML
SUBJECT_SAMPLE_FACTORS           	-	051823_POS_RH70_RW1	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:Rehydration into 200 mM NaCl after 24 hours in 200 mM NaCl	Chromatography=Normal phase formic acid; Mode=Positive; Experimental replicate=Experiment 2; RAW_FILE_NAME(Raw data file names)=051823_POS_RH70_RW1.d; mzXML file names=051823_POS_RH70_RW1.mzXML
SUBJECT_SAMPLE_FACTORS           	-	051823_POS_RH70_RW2	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:Rehydration into 200 mM NaCl after 24 hours in 200 mM NaCl	Chromatography=Normal phase formic acid; Mode=Positive; Experimental replicate=Experiment 2; RAW_FILE_NAME(Raw data file names)=051823_POS_RH70_RW2.d; mzXML file names=051823_POS_RH70_RW2.mzXML
SUBJECT_SAMPLE_FACTORS           	-	051823_POS_RH70_RW3	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:Rehydration into 200 mM NaCl after 24 hours in 200 mM NaCl	Chromatography=Normal phase formic acid; Mode=Positive; Experimental replicate=Experiment 2; RAW_FILE_NAME(Raw data file names)=051823_POS_RH70_RW3.d; mzXML file names=051823_POS_RH70_RW3.mzXML
SUBJECT_SAMPLE_FACTORS           	-	051823_POS_RH70_W11	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:24 hours of incubation in 200 mM NaCl	Chromatography=Normal phase formic acid; Mode=Positive; Experimental replicate=Experiment 2; RAW_FILE_NAME(Raw data file names)=051823_POS_RH70_W11.d; mzXML file names=051823_POS_RH70_W11.mzXML
SUBJECT_SAMPLE_FACTORS           	-	051823_POS_RH70_W12	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:24 hours of incubation in 200 mM NaCl	Chromatography=Normal phase formic acid; Mode=Positive; Experimental replicate=Experiment 2; RAW_FILE_NAME(Raw data file names)=051823_POS_RH70_W12.d; mzXML file names=051823_POS_RH70_W12.mzXML
SUBJECT_SAMPLE_FACTORS           	-	051823_POS_RH70_W13	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:24 hours of incubation in 200 mM NaCl	Chromatography=Normal phase formic acid; Mode=Positive; Experimental replicate=Experiment 2; RAW_FILE_NAME(Raw data file names)=051823_POS_RH70_W13.d; mzXML file names=051823_POS_RH70_W13.mzXML
SUBJECT_SAMPLE_FACTORS           	-	20220422_RNAseq_D01_Hilic_January_Neg	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:Log phase biofilm growth on 7H10	Chromatography=Hilic; Mode=Negative; Experimental replicate=Experiment 1; RAW_FILE_NAME(Raw data file names)=20220422_RNAseq_D01_Hilic_January_Neg.d; mzXML file names=20220422_RNAseq_D01_Hilic_January_Neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	20220422_RNAseq_D02_Hilic_January_Neg	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:Log phase biofilm growth on 7H10	Chromatography=Hilic; Mode=Negative; Experimental replicate=Experiment 1; RAW_FILE_NAME(Raw data file names)=20220422_RNAseq_D02_Hilic_January_Neg.d; mzXML file names=20220422_RNAseq_D02_Hilic_January_Neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	20220422_RNAseq_D03_Hilic_January_Neg	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:Log phase biofilm growth on 7H10	Chromatography=Hilic; Mode=Negative; Experimental replicate=Experiment 1; RAW_FILE_NAME(Raw data file names)=20220422_RNAseq_D03_Hilic_January_Neg.d; mzXML file names=20220422_RNAseq_D03_Hilic_January_Neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	20220422_RNAseq_D11_Hilic_January_Neg	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:24 hours of desiccation from biofilm	Chromatography=Hilic; Mode=Negative; Experimental replicate=Experiment 1; RAW_FILE_NAME(Raw data file names)=20220422_RNAseq_D11_Hilic_January_Neg.d; mzXML file names=20220422_RNAseq_D11_Hilic_January_Neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	20220422_RNAseq_D12_Hilic_January_Neg	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:24 hours of desiccation from biofilm	Chromatography=Hilic; Mode=Negative; Experimental replicate=Experiment 1; RAW_FILE_NAME(Raw data file names)=20220422_RNAseq_D12_Hilic_January_Neg.d; mzXML file names=20220422_RNAseq_D12_Hilic_January_Neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	20220422_RNAseq_D13_Hilic_January_Neg	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:24 hours of desiccation from biofilm	Chromatography=Hilic; Mode=Negative; Experimental replicate=Experiment 1; RAW_FILE_NAME(Raw data file names)=20220422_RNAseq_D13_Hilic_January_Neg.d; mzXML file names=20220422_RNAseq_D13_Hilic_January_Neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	20220422_RNAseq_RD1_Hilic_January_Neg	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:Rehydration into 200 mM NaCl after 24 hours of desiccation	Chromatography=Hilic; Mode=Negative; Experimental replicate=Experiment 1; RAW_FILE_NAME(Raw data file names)=20220422_RNAseq_RD1_Hilic_January_Neg.d; mzXML file names=20220422_RNAseq_RD1_Hilic_January_Neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	20220422_RNAseq_RD2_Hilic_January_Neg	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:Rehydration into 200 mM NaCl after 24 hours of desiccation	Chromatography=Hilic; Mode=Negative; Experimental replicate=Experiment 1; RAW_FILE_NAME(Raw data file names)=20220422_RNAseq_RD2_Hilic_January_Neg.d; mzXML file names=20220422_RNAseq_RD2_Hilic_January_Neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	20220422_RNAseq_RD3_Hilic_January_Neg	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:Rehydration into 200 mM NaCl after 24 hours of desiccation	Chromatography=Hilic; Mode=Negative; Experimental replicate=Experiment 1; RAW_FILE_NAME(Raw data file names)=20220422_RNAseq_RD3_Hilic_January_Neg.d; mzXML file names=20220422_RNAseq_RD3_Hilic_January_Neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	20220422_RNAseq_RW1_Hilic_January_Neg	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:Rehydration into 200 mM NaCl after 24 hours in 200 mM NaCl	Chromatography=Hilic; Mode=Negative; Experimental replicate=Experiment 1; RAW_FILE_NAME(Raw data file names)=20220422_RNAseq_RW1_Hilic_January_Neg.d; mzXML file names=20220422_RNAseq_RW1_Hilic_January_Neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	20220422_RNAseq_RW2_Hilic_January_Neg	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:Rehydration into 200 mM NaCl after 24 hours in 200 mM NaCl	Chromatography=Hilic; Mode=Negative; Experimental replicate=Experiment 1; RAW_FILE_NAME(Raw data file names)=20220422_RNAseq_RW2_Hilic_January_Neg.d; mzXML file names=20220422_RNAseq_RW2_Hilic_January_Neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	20220422_RNAseq_RW3_Hilic_January_Neg	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:Rehydration into 200 mM NaCl after 24 hours in 200 mM NaCl	Chromatography=Hilic; Mode=Negative; Experimental replicate=Experiment 1; RAW_FILE_NAME(Raw data file names)=20220422_RNAseq_RW3_Hilic_January_Neg.d; mzXML file names=20220422_RNAseq_RW3_Hilic_January_Neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	20220422_RNAseq_W11_Hilic_January_Neg	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:24 hours of incubation in 200 mM NaCl	Chromatography=Hilic; Mode=Negative; Experimental replicate=Experiment 1; RAW_FILE_NAME(Raw data file names)=20220422_RNAseq_W11_Hilic_January_Neg.d; mzXML file names=20220422_RNAseq_W11_Hilic_January_Neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	20220422_RNAseq_W12_Hilic_January_Neg	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:24 hours of incubation in 200 mM NaCl	Chromatography=Hilic; Mode=Negative; Experimental replicate=Experiment 1; RAW_FILE_NAME(Raw data file names)=20220422_RNAseq_W12_Hilic_January_Neg.d; mzXML file names=20220422_RNAseq_W12_Hilic_January_Neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	20220422_RNAseq_W13_Hilic_January_Neg	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:24 hours of incubation in 200 mM NaCl	Chromatography=Hilic; Mode=Negative; Experimental replicate=Experiment 1; RAW_FILE_NAME(Raw data file names)=20220422_RNAseq_W13_Hilic_January_Neg.d; mzXML file names=20220422_RNAseq_W13_Hilic_January_Neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	20250113_Hilic_RH70_D01	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:Log phase biofilm growth on 7H10	Chromatography=Hilic; Mode=Negative; Experimental replicate=Experiment 2; RAW_FILE_NAME(Raw data file names)=20250113_Hilic_RH70_D01.d; mzXML file names=20250113_Hilic_RH70_D01.mzXML
SUBJECT_SAMPLE_FACTORS           	-	20250113_Hilic_RH70_D02	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:Log phase biofilm growth on 7H10	Chromatography=Hilic; Mode=Negative; Experimental replicate=Experiment 2; RAW_FILE_NAME(Raw data file names)=20250113_Hilic_RH70_D02.d; mzXML file names=20250113_Hilic_RH70_D02.mzXML
SUBJECT_SAMPLE_FACTORS           	-	20250113_Hilic_RH70_D03	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:Log phase biofilm growth on 7H10	Chromatography=Hilic; Mode=Negative; Experimental replicate=Experiment 2; RAW_FILE_NAME(Raw data file names)=20250113_Hilic_RH70_D03.d; mzXML file names=20250113_Hilic_RH70_D03.mzXML
SUBJECT_SAMPLE_FACTORS           	-	20250113_Hilic_RH70_D11	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:24 hours of desiccation from biofilm	Chromatography=Hilic; Mode=Negative; Experimental replicate=Experiment 2; RAW_FILE_NAME(Raw data file names)=20250113_Hilic_RH70_D11.d; mzXML file names=20250113_Hilic_RH70_D11.mzXML
SUBJECT_SAMPLE_FACTORS           	-	20250113_Hilic_RH70_D12	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:24 hours of desiccation from biofilm	Chromatography=Hilic; Mode=Negative; Experimental replicate=Experiment 2; RAW_FILE_NAME(Raw data file names)=20250113_Hilic_RH70_D12.d; mzXML file names=20250113_Hilic_RH70_D12.mzXML
SUBJECT_SAMPLE_FACTORS           	-	20250113_Hilic_RH70_D13	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:24 hours of desiccation from biofilm	Chromatography=Hilic; Mode=Negative; Experimental replicate=Experiment 2; RAW_FILE_NAME(Raw data file names)=20250113_Hilic_RH70_D13.d; mzXML file names=20250113_Hilic_RH70_D13.mzXML
SUBJECT_SAMPLE_FACTORS           	-	20250113_Hilic_RH70_RD1	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:Rehydration into 200 mM NaCl after 24 hours of desiccation	Chromatography=Hilic; Mode=Negative; Experimental replicate=Experiment 2; RAW_FILE_NAME(Raw data file names)=20250113_Hilic_RH70_RD1.d; mzXML file names=20250113_Hilic_RH70_RD1.mzXML
SUBJECT_SAMPLE_FACTORS           	-	20250113_Hilic_RH70_RD2	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:Rehydration into 200 mM NaCl after 24 hours of desiccation	Chromatography=Hilic; Mode=Negative; Experimental replicate=Experiment 2; RAW_FILE_NAME(Raw data file names)=20250113_Hilic_RH70_RD2.d; mzXML file names=20250113_Hilic_RH70_RD2.mzXML
SUBJECT_SAMPLE_FACTORS           	-	20250113_Hilic_RH70_RD3	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:Rehydration into 200 mM NaCl after 24 hours of desiccation	Chromatography=Hilic; Mode=Negative; Experimental replicate=Experiment 2; RAW_FILE_NAME(Raw data file names)=20250113_Hilic_RH70_RD3.d; mzXML file names=20250113_Hilic_RH70_RD3.mzXML
SUBJECT_SAMPLE_FACTORS           	-	20250113_Hilic_RH70_RW1	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:Rehydration into 200 mM NaCl after 24 hours in 200 mM NaCl	Chromatography=Hilic; Mode=Negative; Experimental replicate=Experiment 2; RAW_FILE_NAME(Raw data file names)=20250113_Hilic_RH70_RW1.d; mzXML file names=20250113_Hilic_RH70_RW1.mzXML
SUBJECT_SAMPLE_FACTORS           	-	20250113_Hilic_RH70_RW2	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:Rehydration into 200 mM NaCl after 24 hours in 200 mM NaCl	Chromatography=Hilic; Mode=Negative; Experimental replicate=Experiment 2; RAW_FILE_NAME(Raw data file names)=20250113_Hilic_RH70_RW2.d; mzXML file names=20250113_Hilic_RH70_RW2.mzXML
SUBJECT_SAMPLE_FACTORS           	-	20250113_Hilic_RH70_RW3	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:Rehydration into 200 mM NaCl after 24 hours in 200 mM NaCl	Chromatography=Hilic; Mode=Negative; Experimental replicate=Experiment 2; RAW_FILE_NAME(Raw data file names)=20250113_Hilic_RH70_RW3.d; mzXML file names=20250113_Hilic_RH70_RW3.mzXML
SUBJECT_SAMPLE_FACTORS           	-	20250113_Hilic_RH70_W11	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:24 hours of incubation in 200 mM NaCl	Chromatography=Hilic; Mode=Negative; Experimental replicate=Experiment 2; RAW_FILE_NAME(Raw data file names)=20250113_Hilic_RH70_W11.d; mzXML file names=20250113_Hilic_RH70_W11.mzXML
SUBJECT_SAMPLE_FACTORS           	-	20250113_Hilic_RH70_W12	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:24 hours of incubation in 200 mM NaCl	Chromatography=Hilic; Mode=Negative; Experimental replicate=Experiment 2; RAW_FILE_NAME(Raw data file names)=20250113_Hilic_RH70_W12.d; mzXML file names=20250113_Hilic_RH70_W12.mzXML
SUBJECT_SAMPLE_FACTORS           	-	20250113_Hilic_RH70_W13	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:24 hours of incubation in 200 mM NaCl	Chromatography=Hilic; Mode=Negative; Experimental replicate=Experiment 2; RAW_FILE_NAME(Raw data file names)=20250113_Hilic_RH70_W13.d; mzXML file names=20250113_Hilic_RH70_W13.mzXML
#COLLECTION
CO:COLLECTION_SUMMARY            	Laboratory strain H37Rv was grown as a biofilm on Middlebrook 7H10 agar
CO:COLLECTION_SUMMARY            	supplemented with glucose, sodium chloride, fatty-acid free albumin and
CO:COLLECTION_SUMMARY            	glycerol. Cells were plunged into a 40/40/20 mixture of methanol, acetonitrile
CO:COLLECTION_SUMMARY            	and mili-Q water followed by lysis. Stored at -80 C until analysis by LC-MS.
CO:SAMPLE_TYPE                   	Cultured cells
CO:STORAGE_CONDITIONS            	-80℃
#TREATMENT
TR:TREATMENT_SUMMARY             	We analyzed the effect of desiccation on Mycobacterium tuberculosis (Mtb)
TR:TREATMENT_SUMMARY             	metabolism. Laboratory strain H37Rv was grown as a biofilm on Middlebrook 7H10
TR:TREATMENT_SUMMARY             	agar supplemented with glucose, sodium chloride, fatty-acid free albumin and
TR:TREATMENT_SUMMARY             	glycerol. Biofilms were incubated for 24 hours at 37 °C in 70% relative
TR:TREATMENT_SUMMARY             	humidity over liquid reservoirs containing 200 mM NaCl (wet condition) or left
TR:TREATMENT_SUMMARY             	empty (dry condition). Following 24 hours, wet and dry conditions were
TR:TREATMENT_SUMMARY             	rehydrated in 200 mM NaCl and incubated a further 24 hours. Each experiment
TR:TREATMENT_SUMMARY             	included 3 biological replicates and was performed twice. LC-MS metabolomics
TR:TREATMENT_SUMMARY             	revealed significant changes to central and secondary carbon metabolism.
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	Cell-laden filters were plunged into 2 mL screw cap threaded tubes with O-ring
SP:SAMPLEPREP_SUMMARY            	seal (Sarstedt) containing 1mL of a 2:2:1 HPLC-grade Methanol:acetonitrile:water
SP:SAMPLEPREP_SUMMARY            	(Sigma-Aldrich) solution. Cells were lysed in an air-chilled Precellys
SP:SAMPLEPREP_SUMMARY            	homogenizer at 6000 rpm x 3 cycles x 30 seconds per cycle with 30 seconds rest
SP:SAMPLEPREP_SUMMARY            	in between to prevent sample overheating. Lysates were filtered through a 0.22
SP:SAMPLEPREP_SUMMARY            	µm column to ensure sterilization before removal from BSL3 laboratory. 100 µL
SP:SAMPLEPREP_SUMMARY            	aliquot of lysate was combined with 100 µL of ddH2O containing 0.2% formic acid
SP:SAMPLEPREP_SUMMARY            	and centrifuged. The supernatant was analyzed by LC-MS.
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_SUMMARY        	The metabolite admixture was chromatographically separated using hydrophilic
CH:CHROMATOGRAPHY_SUMMARY        	interaction chromatography (HILIC)on a Poroshell 120 Hilic-Z column (150 mm)
CH:CHROMATOGRAPHY_SUMMARY        	using a gradient mobile phase (flow rate 0.25 mL/min) composed of Solvent A
CH:CHROMATOGRAPHY_SUMMARY        	(10mM ammonium acetate in ddH2O with 5 µM Infinity Lab deactivator (Agilent))
CH:CHROMATOGRAPHY_SUMMARY        	and Solvent B (95:15 Acetonitrile:ddH2O with 10 mM ammonium acetate and 5 µM
CH:CHROMATOGRAPHY_SUMMARY        	Infinity Lab deactivator). Starting composition of solvents was 4% Solvent A and
CH:CHROMATOGRAPHY_SUMMARY        	96% Solvent B and rising to 35% Solvent A and 65% Solvent B over 24 minutes.
CH:CHROMATOGRAPHY_TYPE           	HILIC
CH:INSTRUMENT_NAME               	Agilent QTOF
CH:COLUMN_NAME                   	Agilent Infinity Lab Poroshell 120 Hilic-Z (150 x 2.1mm, 2.7um)
CH:SOLVENT_A                     	100% water; 10mM ammonium acetate; 5 µM Infinity Lab deactivator (Agilent)
CH:SOLVENT_B                     	85% Acetonitrile/15% water; 10 mM ammonium acetate; 5 µM Infinity Lab
CH:SOLVENT_B                     	deactivator
CH:FLOW_GRADIENT                 	96% B to 65% B linear gradient over 24 minutes
CH:FLOW_RATE                     	0.25 mL/min
CH:COLUMN_TEMPERATURE            	25 C
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
#MS
MS:INSTRUMENT_NAME               	Agilent Revident Q-TOF
MS:INSTRUMENT_TYPE               	QTOF
MS:MS_TYPE                       	ESI
MS:ION_MODE                      	NEGATIVE
MS:MS_COMMENTS                   	Following chromatography the sample underwent electrospray ionization on an
MS:MS_COMMENTS                   	Agilent Revident ESI-QTOF. Dry gas temperature was set to 300 °C, gas flow 3.5
MS:MS_COMMENTS                   	L/min, nebulizer pressure 35 psi, and capillary voltage of 3500 V. Ions were
MS:MS_COMMENTS                   	detected in negative mode (m/z range: 50 – 1700). Internal calibrants
MS:MS_COMMENTS                   	62.032683 and 983.035752 were infused continuously. Data are reported in units
MS:MS_COMMENTS                   	of "AUC per CFU (colony forming units} per mL"
#MS_METABOLITE_DATA
MS_METABOLITE_DATA:UNITS	AUC/CFU per mL
MS_METABOLITE_DATA_START
Samples	20220422_RNAseq_D01_Hilic_January_Neg	20220422_RNAseq_D02_Hilic_January_Neg	20220422_RNAseq_D03_Hilic_January_Neg	20220422_RNAseq_D11_Hilic_January_Neg	20220422_RNAseq_D12_Hilic_January_Neg	20220422_RNAseq_D13_Hilic_January_Neg	20220422_RNAseq_RD1_Hilic_January_Neg	20220422_RNAseq_RD2_Hilic_January_Neg	20220422_RNAseq_RD3_Hilic_January_Neg	20220422_RNAseq_W11_Hilic_January_Neg	20220422_RNAseq_W12_Hilic_January_Neg	20220422_RNAseq_W13_Hilic_January_Neg	20220422_RNAseq_RW1_Hilic_January_Neg	20220422_RNAseq_RW2_Hilic_January_Neg	20220422_RNAseq_RW3_Hilic_January_Neg	20250113_Hilic_RH70_D01	20250113_Hilic_RH70_D02	20250113_Hilic_RH70_D03	20250113_Hilic_RH70_D11	20250113_Hilic_RH70_D12	20250113_Hilic_RH70_D13	20250113_Hilic_RH70_RD1	20250113_Hilic_RH70_RD2	20250113_Hilic_RH70_RD3	20250113_Hilic_RH70_W11	20250113_Hilic_RH70_W12	20250113_Hilic_RH70_W13	20250113_Hilic_RH70_RW1	20250113_Hilic_RH70_RW2	20250113_Hilic_RH70_RW3	20220329_RNAseq_D01_FA_March22_Neg	20220329_RNAseq_D02_FA_March22_Neg	20220329_RNAseq_D03_FA_March22_Neg	20220329_RNAseq_D11_FA_March22_Neg	20220329_RNAseq_D12_FA_March22_Neg	20220329_RNAseq_D13_FA_March22_Neg	20220329_RNAseq_RD1_FA_March22_Neg	20220329_RNAseq_RD2_FA_March22_Neg	20220329_RNAseq_RD3_FA_March22_Neg	20220329_RNAseq_RW1_FA_March22_Neg	20220329_RNAseq_RW2_FA_March22_Neg	20220329_RNAseq_RW3_FA_March22_Neg	20220329_RNAseq_W11_FA_March22_Neg	20220329_RNAseq_W12_FA_March22_Neg	20220329_RNAseq_W13_FA_March22_Neg	051823_NEG_RH70_D01	051823_NEG_RH70_D02	051823_NEG_RH70_D03	051823_NEG_RH70_D11	051823_NEG_RH70_D12	051823_NEG_RH70_D13	051823_NEG_RH70_RD1	051823_NEG_RH70_RD2	051823_NEG_RH70_RD3	051823_NEG_RH70_RW1	051823_NEG_RH70_RW2	051823_NEG_RH70_RW3	051823_NEG_RH70_W11	051823_NEG_RH70_W12	051823_NEG_RH70_W13	20220329_RNAseq_D01_FA_March22_Pos	20220329_RNAseq_D02_FA_March22_Pos	20220329_RNAseq_D03_FA_March22_Pos	20220329_RNAseq_D11_FA_March22_Pos	20220329_RNAseq_D12_FA_March22_Pos	20220329_RNAseq_D13_FA_March22_Pos	20220329_RNAseq_RD1_FA_March22_Pos	20220329_RNAseq_RD2_FA_March22_Pos	20220329_RNAseq_RD3_FA_March22_Pos	20220329_RNAseq_RW1_FA_March22_Pos	20220329_RNAseq_RW2_FA_March22_Pos	20220329_RNAseq_RW3_FA_March22_Pos	20220329_RNAseq_W11_FA_March22_Pos	20220329_RNAseq_W12_FA_March22_Pos	20220329_RNAseq_W13_FA_March22_Pos			
Factors	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:Log phase biofilm growth on 7H10	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:Log phase biofilm growth on 7H10	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:Log phase biofilm growth on 7H10	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:24 hours of desiccation from biofilm	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:24 hours of desiccation from biofilm	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:24 hours of desiccation from biofilm	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:Rehydration into 200 mM NaCl after 24 hours of desiccation	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:Rehydration into 200 mM NaCl after 24 hours of desiccation	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:Rehydration into 200 mM NaCl after 24 hours of desiccation	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:24 hours of incubation in 200 mM NaCl	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:24 hours of incubation in 200 mM NaCl	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:24 hours of incubation in 200 mM NaCl	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:Rehydration into 200 mM NaCl after 24 hours in 200 mM NaCl	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:Rehydration into 200 mM NaCl after 24 hours in 200 mM NaCl	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:Rehydration into 200 mM NaCl after 24 hours in 200 mM NaCl	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:Log phase biofilm growth on 7H10	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:Log phase biofilm growth on 7H10	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:Log phase biofilm growth on 7H10	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:24 hours of desiccation from biofilm	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:24 hours of desiccation from biofilm	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:24 hours of desiccation from biofilm	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:Rehydration into 200 mM NaCl after 24 hours of desiccation	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:Rehydration into 200 mM NaCl after 24 hours of desiccation	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:Rehydration into 200 mM NaCl after 24 hours of desiccation	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:24 hours of incubation in 200 mM NaCl	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:24 hours of incubation in 200 mM NaCl	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:24 hours of incubation in 200 mM NaCl	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:Rehydration into 200 mM NaCl after 24 hours in 200 mM NaCl	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:Rehydration into 200 mM NaCl after 24 hours in 200 mM NaCl	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:Rehydration into 200 mM NaCl after 24 hours in 200 mM NaCl	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:Log phase biofilm growth on 7H10	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:Log phase biofilm growth on 7H10	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:Log phase biofilm growth on 7H10	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:24 hours of desiccation from biofilm	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:24 hours of desiccation from biofilm	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:24 hours of desiccation from biofilm	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:Rehydration into 200 mM NaCl after 24 hours of desiccation	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:Rehydration into 200 mM NaCl after 24 hours of desiccation	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:Rehydration into 200 mM NaCl after 24 hours of desiccation	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:Rehydration into 200 mM NaCl after 24 hours in 200 mM NaCl	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:Rehydration into 200 mM NaCl after 24 hours in 200 mM NaCl	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:Rehydration into 200 mM NaCl after 24 hours in 200 mM NaCl	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:24 hours of incubation in 200 mM NaCl	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:24 hours of incubation in 200 mM NaCl	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:24 hours of incubation in 200 mM NaCl	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:Log phase biofilm growth on 7H10	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:Log phase biofilm growth on 7H10	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:Log phase biofilm growth on 7H10	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:24 hours of desiccation from biofilm	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:24 hours of desiccation from biofilm	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:24 hours of desiccation from biofilm	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:Rehydration into 200 mM NaCl after 24 hours of desiccation	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:Rehydration into 200 mM NaCl after 24 hours of desiccation	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:Rehydration into 200 mM NaCl after 24 hours of desiccation	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:Rehydration into 200 mM NaCl after 24 hours in 200 mM NaCl	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:Rehydration into 200 mM NaCl after 24 hours in 200 mM NaCl	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:Rehydration into 200 mM NaCl after 24 hours in 200 mM NaCl	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:24 hours of incubation in 200 mM NaCl	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:24 hours of incubation in 200 mM NaCl	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:24 hours of incubation in 200 mM NaCl	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:Log phase biofilm growth on 7H10	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:Log phase biofilm growth on 7H10	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:Log phase biofilm growth on 7H10	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:24 hours of desiccation from biofilm	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:24 hours of desiccation from biofilm	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:24 hours of desiccation from biofilm	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:Rehydration into 200 mM NaCl after 24 hours of desiccation	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:Rehydration into 200 mM NaCl after 24 hours of desiccation	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:Rehydration into 200 mM NaCl after 24 hours of desiccation	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:Rehydration into 200 mM NaCl after 24 hours in 200 mM NaCl	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:Rehydration into 200 mM NaCl after 24 hours in 200 mM NaCl	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:Rehydration into 200 mM NaCl after 24 hours in 200 mM NaCl	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:24 hours of incubation in 200 mM NaCl	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:24 hours of incubation in 200 mM NaCl	Sample source:Mycobacterium tuberculosis H37Rv grown in vitro | Growth condition:24 hours of incubation in 200 mM NaCl
Mycothiol	0.499728	0.406662	0.392504	10.96322	8.329193	10.23237	5.78561	4.383788	5.120005	0.450226	0.386545	0.316096	0.546884	0.740083	0.524587	1.176952	1.184938	1.069919	33.71041	42.85179	38.95927	11.7755	12.40979	7.706443	0.7712	0.604815	0.583248	0.536914	0.425312	0.298424	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0			
8-Oxoguanine	0.0000198	0.000037	0.0000391	0.005045	0.003938	0.00347	0.000184	0	0	0	0	0	0.0000231	0	0	0.0000446	0.0000489	0.000045	0.009533	0.009151	0.002687	0	0.000326	0.000259	0	0	0	0	0	0.00000929	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0			
8-Oxo-2'-deoxyguanosine	0	0	0	0.015294	0.003805	0.001277	0.117497	0.137123	0.116311	0.001271	0.000907	0.001027	0.001688	0.002554	0.001395	0	0	0	0.064985	0.095188	0.306601	0.440232	0.308913	0.284311	0.001439	0.001438	0.00256	0.001343	0.001066	0.001399	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0			
Aspartate	0.032576	0.028334	0.026281	20.91533	13.16511	15.01345	2.264279	1.685796	1.958869	0.065954	0.053197	0.062786	0.109434	0.114661	0.10415	0.078794	0.081168	0.090474	59.0311	70.24052	100.1198	4.907692	4.711088	5.875245	0.070077	0.04933	0.064509	0.045184	0.048147	0.045179	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0			
Dihydroorotate	0.002088	0.002096	0.001712	0.090025	0.048009	0.066474	0.012161	0.013292	0.020412	0.001668	0.001727	0.001898	0.003136	0.004558	0.003137	0.003677	0.00372	0.005549	0.464739	0.582985	0.908298	0.047538	0.053063	0.036067	0.002053	0.002274	0.002814	0.001817	0.001805	0.001531	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0			
Orotate	0.001972	0.001944	0.001775	0.180786	0.058291	0.062199	0.031961	0.026406	0.02971	0.001145	0.001218	0.001094	0.001449	0.001722	0.001742	0.002283	0.002081	0.005531	2.137095	3.292214	6.068595	0.151119	0.188648	0.156459	0.001284	0.001204	0.000996	0.001272	0.001354	0.001492	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0			
UMP	0.017909	0.013092	0.010222	1.587458	0.86868	1.070357	0.891075	0.767547	0.905787	0.048594	0.041758	0.036469	0.084881	0.115454	0.066604	0.058086	0.078238	0.163098	7.80052	9.904638	6.22594	1.8678	1.867641	2.063755	0.055653	0.063554	0.06721	0.052464	0.046396	0.065708	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0			
MS_METABOLITE_DATA_END
#METABOLITES
METABOLITES_START
metabolite_name	mz	RT (min)
Mycothiol	485.145	6.83
8-Oxoguanine	166.036	2.83
8-Oxo-2'-deoxyguanosine	282.084	3.19
Aspartate	132.03	9.44
Dihydroorotate	157.025	3.48
Orotate	155.009	2.58
UMP	323.028	9.72
METABOLITES_END
#END