#METABOLOMICS WORKBENCH louisen_20250606_034156 DATATRACK_ID:6002 STUDY_ID:ST003982 ANALYSIS_ID:AN006558 PROJECT_ID:PR002492
VERSION             	1
CREATED_ON             	June 16, 2025, 7:06 pm
#PROJECT
PR:PROJECT_TITLE                 	Unraveling mitochondrial pyruvate dysfunction to mitigate hyperlactatemia and
PR:PROJECT_TITLE                 	lethality in sepsis
PR:PROJECT_SUMMARY               	Sepsis, killing 11 million people yearly, is associated with increased lactate
PR:PROJECT_SUMMARY               	production - a metabolite mechanistically linked to mortality - complicating
PR:PROJECT_SUMMARY               	glucose administration in sepsis. To understand the mechanism behind
PR:PROJECT_SUMMARY               	hyperlactatemia, we applied the cecal ligation and puncture (CLP) model and
PR:PROJECT_SUMMARY               	studied all pyruvate processing routes in liver mitochondria during acute
PR:PROJECT_SUMMARY               	sepsis. Our data suggest that mitochondrial pyruvate-driven respiration is
PR:PROJECT_SUMMARY               	nearly nonexistent in sepsis, not due to insufficient pyruvate uptake or
PR:PROJECT_SUMMARY               	carboxylation but due to a dysfunctional pyruvate dehydrogenase complex (PDC).
PR:PROJECT_SUMMARY               	Septic mitochondria compensate by glutamate-mediated TCA anaplerosis,
PR:PROJECT_SUMMARY               	simultaneously converting some pyruvate into alanine via enhanced mitochondrial
PR:PROJECT_SUMMARY               	glutamic pyruvate transaminase (GPT2) activity. Notably, PDC dysfunction is not
PR:PROJECT_SUMMARY               	caused by PDC inactivation per se but by a shortage of its cofactor, thiamine
PR:PROJECT_SUMMARY               	pyrophosphate (TPP). TPP supplementation restores pyruvate oxidation both ex
PR:PROJECT_SUMMARY               	vivo and in vivo and protects mice from sepsis. TPP also allows safe glucose
PR:PROJECT_SUMMARY               	administration in mice, leading to a novel, robust TPP-plus-glucose therapy.
PR:INSTITUTE                     	Ghent University
PR:DEPARTMENT                    	Inflammation research center - VIB
PR:LABORATORY                    	Mouse Genetics in Inflammation
PR:LAST_NAME                     	Libert
PR:FIRST_NAME                    	Claude
PR:ADDRESS                       	Technologiepark-Zwijnaarde 71, 9052 Ghent, Belgium
PR:EMAIL                         	claude.libert@irc.vib-ugent.be
PR:PHONE                         	09/331.37.00
#STUDY
ST:STUDY_TITLE                   	Mitochondrial pyruvate metabolism is disturbed and contributes to
ST:STUDY_TITLE                   	hyperlactatemia and lethality in sepsis - Liver, Plasma, Skeletal muscle
ST:STUDY_SUMMARY                 	To determine the source of lactate production in sepsis, lactate dehydrogenase A
ST:STUDY_SUMMARY                 	(LDHA) activity was examined in several organs of sham and cecal ligation and
ST:STUDY_SUMMARY                 	puncture (CLP) mice using 13C3 pyruvate tracer metabolomics assay. Septic heart
ST:STUDY_SUMMARY                 	and skeletal muscle did not show an enhanced 13C labeling of lactate, indicating
ST:STUDY_SUMMARY                 	that LDHA activity is not increased in these tissues and that they are unlikely
ST:STUDY_SUMMARY                 	to be major sources of elevated lactate production in sepsis. Conversely, septic
ST:STUDY_SUMMARY                 	liver, kidney, spleen and lung were characterized by a notably higher
ST:STUDY_SUMMARY                 	13C-labeling for lactate, indicating elevated local lactate production that may
ST:STUDY_SUMMARY                 	contribute to systemic hyperlactatemia.
ST:INSTITUTE                     	Ghent University
ST:LAST_NAME                     	Libert
ST:FIRST_NAME                    	Claude
ST:ADDRESS                       	Technologiepark-Zwijnaarde 71, 9052 Ghent, Belgium
ST:EMAIL                         	claude.libert@irc.vib-ugent.be
ST:PHONE                         	09/331.37.00
#SUBJECT
SU:SUBJECT_TYPE                  	Mammal
SU:SUBJECT_SPECIES               	Mus musculus
SU:TAXONOMY_ID                   	10090
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data
SUBJECT_SAMPLE_FACTORS           	-	MCF001167_LN01	Sample source:Plasma | Treatment condition:Sham | Tracer condition:13C pyruvate	RAW_FILE_NAME(Raw file name)=MCF001167_LN01.raw
SUBJECT_SAMPLE_FACTORS           	-	MCF001167_LN02	Sample source:Liver | Treatment condition:Sham | Tracer condition:13C pyruvate	RAW_FILE_NAME(Raw file name)=MCF001167_LN02.raw
SUBJECT_SAMPLE_FACTORS           	-	MCF001167_LN03	Sample source:Skeletal muscle | Treatment condition:Sham | Tracer condition:13C pyruvate	RAW_FILE_NAME(Raw file name)=MCF001167_LN03.raw
SUBJECT_SAMPLE_FACTORS           	-	MCF001167_LN04	Sample source:Plasma | Treatment condition:CLP | Tracer condition:13C pyruvate	RAW_FILE_NAME(Raw file name)=MCF001167_LN04.raw
SUBJECT_SAMPLE_FACTORS           	-	MCF001167_LN05	Sample source:Liver | Treatment condition:CLP | Tracer condition:13C pyruvate	RAW_FILE_NAME(Raw file name)=MCF001167_LN05.raw
SUBJECT_SAMPLE_FACTORS           	-	MCF001167_LN06	Sample source:Skeletal muscle | Treatment condition:CLP | Tracer condition:13C pyruvate	RAW_FILE_NAME(Raw file name)=MCF001167_LN06.raw
SUBJECT_SAMPLE_FACTORS           	-	MCF001167_LN07	Sample source:Plasma | Treatment condition:Sham | Tracer condition:13C pyruvate	RAW_FILE_NAME(Raw file name)=MCF001167_LN07.raw
SUBJECT_SAMPLE_FACTORS           	-	MCF001167_LN08	Sample source:Liver | Treatment condition:Sham | Tracer condition:13C pyruvate	RAW_FILE_NAME(Raw file name)=MCF001167_LN08.raw
SUBJECT_SAMPLE_FACTORS           	-	MCF001167_LN09	Sample source:Skeletal muscle | Treatment condition:Sham | Tracer condition:13C pyruvate	RAW_FILE_NAME(Raw file name)=MCF001167_LN09.raw
SUBJECT_SAMPLE_FACTORS           	-	MCF001167_LN10	Sample source:Plasma | Treatment condition:CLP | Tracer condition:13C pyruvate	RAW_FILE_NAME(Raw file name)=MCF001167_LN10.raw
SUBJECT_SAMPLE_FACTORS           	-	MCF001167_LN11	Sample source:Liver | Treatment condition:CLP | Tracer condition:13C pyruvate	RAW_FILE_NAME(Raw file name)=MCF001167_LN11.raw
SUBJECT_SAMPLE_FACTORS           	-	MCF001167_LN12	Sample source:Skeletal muscle | Treatment condition:CLP | Tracer condition:13C pyruvate	RAW_FILE_NAME(Raw file name)=MCF001167_LN12.raw
SUBJECT_SAMPLE_FACTORS           	-	MCF001167_LN13	Sample source:Plasma | Treatment condition:Sham | Tracer condition:13C pyruvate	RAW_FILE_NAME(Raw file name)=MCF001167_LN13.raw
SUBJECT_SAMPLE_FACTORS           	-	MCF001167_LN14	Sample source:Liver | Treatment condition:Sham | Tracer condition:13C pyruvate	RAW_FILE_NAME(Raw file name)=MCF001167_LN14.raw
SUBJECT_SAMPLE_FACTORS           	-	MCF001167_LN15	Sample source:Skeletal muscle | Treatment condition:Sham | Tracer condition:13C pyruvate	RAW_FILE_NAME(Raw file name)=MCF001167_LN15.raw
SUBJECT_SAMPLE_FACTORS           	-	MCF001167_LN16	Sample source:Plasma | Treatment condition:Sham | Tracer condition:13C pyruvate	RAW_FILE_NAME(Raw file name)=MCF001167_LN16.raw
SUBJECT_SAMPLE_FACTORS           	-	MCF001167_LN17	Sample source:Liver | Treatment condition:Sham | Tracer condition:13C pyruvate	RAW_FILE_NAME(Raw file name)=MCF001167_LN17.raw
SUBJECT_SAMPLE_FACTORS           	-	MCF001167_LN18	Sample source:Skeletal muscle | Treatment condition:Sham | Tracer condition:13C pyruvate	RAW_FILE_NAME(Raw file name)=MCF001167_LN18.raw
SUBJECT_SAMPLE_FACTORS           	-	MCF001167_LN19	Sample source:Plasma | Treatment condition:CLP | Tracer condition:13C pyruvate	RAW_FILE_NAME(Raw file name)=MCF001167_LN19.raw
SUBJECT_SAMPLE_FACTORS           	-	MCF001167_LN20	Sample source:Liver | Treatment condition:CLP | Tracer condition:13C pyruvate	RAW_FILE_NAME(Raw file name)=MCF001167_LN20.raw
SUBJECT_SAMPLE_FACTORS           	-	MCF001167_LN21	Sample source:Skeletal muscle | Treatment condition:CLP | Tracer condition:13C pyruvate	RAW_FILE_NAME(Raw file name)=MCF001167_LN21.raw
SUBJECT_SAMPLE_FACTORS           	-	MCF001167_LN22	Sample source:Plasma | Treatment condition:Sham | Tracer condition:13C pyruvate	RAW_FILE_NAME(Raw file name)=MCF001167_LN22.raw
SUBJECT_SAMPLE_FACTORS           	-	MCF001167_LN23	Sample source:Liver | Treatment condition:Sham | Tracer condition:13C pyruvate	RAW_FILE_NAME(Raw file name)=MCF001167_LN23.raw
SUBJECT_SAMPLE_FACTORS           	-	MCF001167_LN24	Sample source:Skeletal muscle | Treatment condition:Sham | Tracer condition:13C pyruvate	RAW_FILE_NAME(Raw file name)=MCF001167_LN24.raw
SUBJECT_SAMPLE_FACTORS           	-	MCF001167_LN25	Sample source:Plasma | Treatment condition:CLP | Tracer condition:13C pyruvate	RAW_FILE_NAME(Raw file name)=MCF001167_LN25.raw
SUBJECT_SAMPLE_FACTORS           	-	MCF001167_LN26	Sample source:Liver | Treatment condition:CLP | Tracer condition:13C pyruvate	RAW_FILE_NAME(Raw file name)=MCF001167_LN26.raw
SUBJECT_SAMPLE_FACTORS           	-	MCF001167_LN27	Sample source:Skeletal muscle | Treatment condition:CLP | Tracer condition:13C pyruvate	RAW_FILE_NAME(Raw file name)=MCF001167_LN27.raw
SUBJECT_SAMPLE_FACTORS           	-	MCF001167_LN28	Sample source:Plasma | Treatment condition:CLP | Tracer condition:13C pyruvate	RAW_FILE_NAME(Raw file name)=MCF001167_LN28.raw
SUBJECT_SAMPLE_FACTORS           	-	MCF001167_LN29	Sample source:Liver | Treatment condition:CLP | Tracer condition:13C pyruvate	RAW_FILE_NAME(Raw file name)=MCF001167_LN29.raw
SUBJECT_SAMPLE_FACTORS           	-	MCF001167_LN30	Sample source:Skeletal muscle | Treatment condition:CLP | Tracer condition:13C pyruvate	RAW_FILE_NAME(Raw file name)=MCF001167_LN30.raw
SUBJECT_SAMPLE_FACTORS           	-	MCF001167_LN31	Sample source:Plasma | Treatment condition:CLP | Tracer condition:PBS	RAW_FILE_NAME(Raw file name)=MCF001167_LN31.raw
SUBJECT_SAMPLE_FACTORS           	-	MCF001167_LN32	Sample source:Liver | Treatment condition:CLP | Tracer condition:PBS	RAW_FILE_NAME(Raw file name)=MCF001167_LN32.raw
SUBJECT_SAMPLE_FACTORS           	-	MCF001167_LN33	Sample source:Skeletal muscle | Treatment condition:CLP | Tracer condition:PBS	RAW_FILE_NAME(Raw file name)=MCF001167_LN33.raw
SUBJECT_SAMPLE_FACTORS           	-	MCF001167_LN34	Sample source:Plasma | Treatment condition:Sham | Tracer condition:PBS	RAW_FILE_NAME(Raw file name)=MCF001167_LN34.raw
SUBJECT_SAMPLE_FACTORS           	-	MCF001167_LN35	Sample source:Liver | Treatment condition:Sham | Tracer condition:PBS	RAW_FILE_NAME(Raw file name)=MCF001167_LN35.raw
SUBJECT_SAMPLE_FACTORS           	-	MCF001167_LN36	Sample source:Skeletal muscle | Treatment condition:Sham | Tracer condition:PBS	RAW_FILE_NAME(Raw file name)=MCF001167_LN36.raw
#COLLECTION
CO:COLLECTION_SUMMARY            	Mice were subjected to the cecal ligation and puncture (CLP) (n=5) to induce
CO:COLLECTION_SUMMARY            	polymicrobial sepsis and the sham (control) procedure (n=5). After 24h, mice
CO:COLLECTION_SUMMARY            	were injected with 13C3, 99% sodium pyruvate (2 g/kg; Buchem B.V. CLM-2440-0.5)
CO:COLLECTION_SUMMARY            	or PBS and 90 minutes after injection organs of interest (liver, skeletal muscle
CO:COLLECTION_SUMMARY            	and plasma) were isolated and snapfrozen in liquid nitrogen and stored at -80°C
CO:COLLECTION_SUMMARY            	for further analysis.
CO:SAMPLE_TYPE                   	Liver, Plasma, Skeletal muscle
CO:ADDITIVES                     	Additional sample source/type: muscle and plasma
#TREATMENT
TR:TREATMENT_SUMMARY             	Mice were subjected to the cecal ligation and puncture (CLP) (n=5), as described
TR:TREATMENT_SUMMARY             	by Rittirsch et al. (Nature Protocols: 2009;4(1):31-6. doi:
TR:TREATMENT_SUMMARY             	10.1038/nprot.2008.214), to induce polymicrobial sepsis and the sham (control,
TR:TREATMENT_SUMMARY             	n=5) procedure. After 24h, mice were injected with 13C3, 99% sodium pyruvate (2
TR:TREATMENT_SUMMARY             	g/kg; Buchem B.V. CLM-2440-0.5) or PBS and 90 minutes after injection organs of
TR:TREATMENT_SUMMARY             	interest (liver, skeletal muscle and plasma) were isolated and snapfrozen in
TR:TREATMENT_SUMMARY             	liquid nitrogen and stored at -80°C for further analysis.
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	Liver, skeletal muscle and plasma were isolated 90 minutes after 13C pyruvate or
SP:SAMPLEPREP_SUMMARY            	PBS injection, snapfrozen in liquid nitrogen and stored at -80°C until further
SP:SAMPLEPREP_SUMMARY            	analysis by the VIB metabolomics core (KU Leuven, Belgium).
SP:PROCESSING_STORAGE_CONDITIONS 	-80℃
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_TYPE           	HILIC
CH:INSTRUMENT_NAME               	Thermo Dionex Ultimate 3000
CH:COLUMN_NAME                   	Agilent InfinityLab Poroshell 120 HILIC-Z (150 x 2.1mm, 2.7um)
CH:SOLVENT_A                     	100% acetonitrile
CH:SOLVENT_B                     	100% water; 10 mM sodium acetate (pH 9.3)
CH:FLOW_GRADIENT                 	Start: 90% A / 10% B; 2–12 min: linear increase to 60% B; 12–15 min: held at
CH:FLOW_GRADIENT                 	60% B; 15–25 min: decreased back to 10% B
CH:FLOW_RATE                     	0.25 mL/min
CH:COLUMN_TEMPERATURE            	25°C
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
#MS
MS:INSTRUMENT_NAME               	Thermo Q Exactive Orbitrap
MS:INSTRUMENT_TYPE               	Orbitrap
MS:MS_TYPE                       	ESI
MS:ION_MODE                      	NEGATIVE
MS:MS_COMMENTS                   	- Mass spectrometry was performed in full scan mode (range 70–1050 m/z) using
MS:MS_COMMENTS                   	negative electrospray ionization. The spray voltage was 2.8 kV; capillary
MS:MS_COMMENTS                   	temperature 320°C; sheath gas 45; auxiliary gas 10; AGC target 3.0E+06;
MS:MS_COMMENTS                   	resolution 70,000. Data were acquired using Xcalibur software (Thermo
MS:MS_COMMENTS                   	Scientific). - Raw intensities were extracted for each metabolite and corrected
MS:MS_COMMENTS                   	for natural ¹³C abundance to calculate fractional contribution and
MS:MS_COMMENTS                   	isotopologue enrichment. Detection limits were estimated using blanks. Data
MS:MS_COMMENTS                   	processing included isotopologue deconvolution and signal normalization. -
MS:MS_COMMENTS                   	Xcalibur (Thermo Scientific) for data acquisition
#MS_METABOLITE_DATA
MS_METABOLITE_DATA:UNITS	peak intensity
MS_METABOLITE_DATA_START
Samples	MCF001167_LN01	MCF001167_LN04	MCF001167_LN07	MCF001167_LN10	MCF001167_LN13	MCF001167_LN16	MCF001167_LN19	MCF001167_LN22	MCF001167_LN25	MCF001167_LN28	MCF001167_LN31	MCF001167_LN34	MCF001167_LN02	MCF001167_LN05	MCF001167_LN08	MCF001167_LN11	MCF001167_LN14	MCF001167_LN17	MCF001167_LN20	MCF001167_LN23	MCF001167_LN26	MCF001167_LN29	MCF001167_LN32	MCF001167_LN35	MCF001167_LN03	MCF001167_LN06	MCF001167_LN09	MCF001167_LN12	MCF001167_LN15	MCF001167_LN18	MCF001167_LN21	MCF001167_LN24	MCF001167_LN27	MCF001167_LN30	MCF001167_LN33	MCF001167_LN36
Factors	Sample source:Plasma | Treatment condition:Sham | Tracer condition:13C pyruvate	Sample source:Plasma | Treatment condition:CLP | Tracer condition:13C pyruvate	Sample source:Plasma | Treatment condition:Sham | Tracer condition:13C pyruvate	Sample source:Plasma | Treatment condition:CLP | Tracer condition:13C pyruvate	Sample source:Plasma | Treatment condition:Sham | Tracer condition:13C pyruvate	Sample source:Plasma | Treatment condition:Sham | Tracer condition:13C pyruvate	Sample source:Plasma | Treatment condition:CLP | Tracer condition:13C pyruvate	Sample source:Plasma | Treatment condition:Sham | Tracer condition:13C pyruvate	Sample source:Plasma | Treatment condition:CLP | Tracer condition:13C pyruvate	Sample source:Plasma | Treatment condition:CLP | Tracer condition:13C pyruvate	Sample source:Plasma | Treatment condition:CLP | Tracer condition:PBS	Sample source:Plasma | Treatment condition:Sham | Tracer condition:PBS	Sample source:Liver | Treatment condition:Sham | Tracer condition:13C pyruvate	Sample source:Liver | Treatment condition:CLP | Tracer condition:13C pyruvate	Sample source:Liver | Treatment condition:Sham | Tracer condition:13C pyruvate	Sample source:Liver | Treatment condition:CLP | Tracer condition:13C pyruvate	Sample source:Liver | Treatment condition:Sham | Tracer condition:13C pyruvate	Sample source:Liver | Treatment condition:Sham | Tracer condition:13C pyruvate	Sample source:Liver | Treatment condition:CLP | Tracer condition:13C pyruvate	Sample source:Liver | Treatment condition:Sham | Tracer condition:13C pyruvate	Sample source:Liver | Treatment condition:CLP | Tracer condition:13C pyruvate	Sample source:Liver | Treatment condition:CLP | Tracer condition:13C pyruvate	Sample source:Liver | Treatment condition:CLP | Tracer condition:PBS	Sample source:Liver | Treatment condition:Sham | Tracer condition:PBS	Sample source:Skeletal muscle | Treatment condition:Sham | Tracer condition:13C pyruvate	Sample source:Skeletal muscle | Treatment condition:CLP | Tracer condition:13C pyruvate	Sample source:Skeletal muscle | Treatment condition:Sham | Tracer condition:13C pyruvate	Sample source:Skeletal muscle | Treatment condition:CLP | Tracer condition:13C pyruvate	Sample source:Skeletal muscle | Treatment condition:Sham | Tracer condition:13C pyruvate	Sample source:Skeletal muscle | Treatment condition:Sham | Tracer condition:13C pyruvate	Sample source:Skeletal muscle | Treatment condition:CLP | Tracer condition:13C pyruvate	Sample source:Skeletal muscle | Treatment condition:Sham | Tracer condition:13C pyruvate	Sample source:Skeletal muscle | Treatment condition:CLP | Tracer condition:13C pyruvate	Sample source:Skeletal muscle | Treatment condition:CLP | Tracer condition:13C pyruvate	Sample source:Skeletal muscle | Treatment condition:CLP | Tracer condition:PBS	Sample source:Skeletal muscle | Treatment condition:Sham | Tracer condition:PBS
L-Lactic acid_PARENT	522E+10	415E+10	747E+10	706E+10	596E+10	581E+10	397E+10	454E+10	588E+10	690E+10	408E+10	595E+10	324E+11	249E+11	507E+11	397E+11	345E+11	389E+11	227E+11	247E+11	268E+11	499E+11	360E+11	501E+11	570E+11	383E+11	710E+11	363E+11	620E+11	670E+11	474E+11	655E+11	637E+11	543E+11	556E+11	746E+11
L-Lactic acid_C13-label-1	403E+09	710E+09	569E+09	103E+10	561E+09	531E+09	647E+09	461E+09	841E+09	911E+09	141E+09	221E+09	215E+10	476E+10	315E+10	549E+10	238E+10	356E+10	386E+10	219E+10	418E+10	606E+10	126E+10	188E+10	311E+10	316E+10	426E+10	327E+10	405E+10	503E+10	305E+10	363E+10	312E+10	387E+10	195E+10	269E+10
L-Lactic acid_C13-label-2	185E+09	457E+09	273E+09	612E+09	309E+09	288E+09	382E+09	288E+09	454E+09	574E+09	000E+00	101E+08	753E+09	274E+10	995E+09	282E+10	902E+09	168E+10	195E+10	110E+10	203E+10	323E+10	131E+08	878E+08	133E+10	158E+10	205E+10	164E+10	210E+10	302E+10	113E+10	161E+10	667E+09	188E+10	000E+00	777E+08
L-Lactic acid_C13-label-3	328E+09	596E+09	481E+09	970E+09	458E+09	461E+09	479E+09	464E+09	737E+09	966E+09	000E+00	528E+07	121E+10	271E+10	147E+10	334E+10	106E+10	182E+10	181E+10	146E+10	308E+10	410E+10	000E+00	451E+08	308E+10	370E+10	388E+10	391E+10	434E+10	552E+10	255E+10	511E+10	114E+10	467E+10	000E+00	388E+08
Pyruvic acid_PARENT	584E+08	363E+08	184E+09	449E+08	205E+09	156E+09	860E+08	134E+09	401E+08	236E+09	908E+08	245E+09	628E+09	456E+09	860E+09	498E+09	596E+09	900E+09	902E+09	819E+09	853E+09	226E+10	938E+09	111E+10	417E+10	134E+10	656E+10	767E+09	416E+10	587E+10	939E+09	406E+10	367E+10	337E+10	120E+10	382E+10
Pyruvic acid_C13-label-1	442E+07	449E+07	134E+08	552E+07	182E+08	134E+08	131E+08	129E+08	475E+07	299E+08	262E+07	835E+07	283E+08	492E+08	387E+08	415E+08	280E+08	528E+08	887E+08	451E+08	968E+08	182E+09	263E+08	341E+08	227E+09	110E+09	391E+09	672E+08	267E+09	432E+09	590E+08	223E+09	184E+09	244E+09	380E+08	133E+09
Pyruvic acid_C13-label-2	182E+07	278E+07	623E+07	343E+07	101E+08	721E+07	776E+07	802E+07	260E+07	192E+08	500E+04	351E+06	581E+07	235E+08	785E+07	175E+08	695E+07	187E+08	332E+08	149E+08	337E+08	710E+08	000E+00	551E+06	965E+08	545E+08	188E+09	334E+08	139E+09	260E+09	217E+08	101E+09	432E+08	123E+09	000E+00	367E+07
Pyruvic acid_C13-label-3	353E+07	427E+07	113E+08	644E+07	154E+08	119E+08	106E+08	134E+08	487E+07	335E+08	000E+00	000E+00	717E+07	206E+08	102E+08	223E+08	653E+07	160E+08	235E+08	119E+08	343E+08	916E+08	000E+00	000E+00	227E+09	118E+09	346E+09	793E+08	286E+09	473E+09	469E+08	313E+09	681E+08	297E+09	000E+00	223E+07
Citric acid_PARENT	960E+09	319E+09	375E+09	427E+09	405E+09	342E+09	248E+09	324E+09	299E+09	661E+09	285E+09	292E+09	943E+09	175E+10	120E+10	830E+09	967E+09	683E+09	250E+10	859E+09	154E+10	264E+10	602E+09	735E+09	242E+09	512E+09	575E+09	541E+09	149E+10	141E+10	887E+09	887E+09	462E+09	102E+10	745E+09	101E+10
Citric acid_C13-label-1	907E+08	445E+08	507E+08	104E+09	632E+08	524E+08	456E+08	499E+08	651E+08	196E+09	479E+08	355E+08	125E+09	666E+09	194E+09	255E+09	168E+09	117E+09	852E+09	166E+09	594E+09	960E+09	836E+08	734E+08	319E+08	132E+09	107E+09	172E+09	296E+09	295E+09	211E+09	159E+09	118E+09	309E+09	754E+08	961E+08
Citric acid_C13-label-2	135E+08	162E+08	158E+08	683E+08	223E+08	176E+08	188E+08	158E+08	406E+08	136E+09	241E+08	127E+08	336E+08	319E+09	603E+08	121E+09	531E+08	357E+08	364E+09	555E+08	354E+09	484E+09	217E+08	958E+07	130E+08	714E+08	507E+08	109E+09	148E+09	148E+09	102E+09	834E+08	554E+08	182E+09	166E+08	149E+08
Citric acid_C13-label-3	441E+07	654E+07	519E+07	245E+08	779E+07	612E+07	681E+07	578E+07	141E+08	720E+08	121E+08	623E+07	635E+07	112E+09	135E+08	368E+08	161E+08	898E+07	128E+09	174E+08	150E+09	195E+09	913E+07	398E+07	385E+07	224E+08	116E+08	336E+08	451E+08	452E+08	293E+08	202E+08	152E+08	701E+08	654E+07	567E+07
Citric acid_C13-label-4	104E+07	147E+07	109E+07	657E+07	144E+07	109E+07	122E+07	106E+07	330E+07	209E+08	169E+07	605E+06	758E+06	173E+08	200E+07	609E+07	295E+07	108E+07	175E+08	178E+07	281E+08	377E+08	877E+06	346E+06	550E+06	427E+07	182E+07	627E+07	107E+08	102E+08	514E+07	340E+07	186E+07	152E+08	787E+06	707E+06
Citric acid_C13-label-5	432E+06	348E+06	370E+06	194E+07	467E+06	352E+06	258E+06	325E+06	102E+07	713E+07	628E+06	000E+00	000E+00	219E+07	000E+00	127E+07	961E+06	000E+00	198E+07	408E+06	512E+07	711E+07	000E+00	000E+00	000E+00	923E+06	000E+00	152E+07	375E+07	394E+07	124E+07	793E+06	000E+00	418E+07	000E+00	000E+00
Citric acid_C13-label-6	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	217E+07	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	161E+07	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	137E+07	124E+07	000E+00	000E+00	000E+00	793E+06	000E+00	000E+00
Fumaric acid_PARENT	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	428E+06	000E+00	000E+00	000E+00	000E+00	122E+09	174E+09	294E+09	167E+09	205E+09	234E+09	284E+09	204E+09	222E+09	311E+09	282E+09	263E+09	000E+00	255E+08	495E+08	185E+08	303E+08	410E+08	312E+08	382E+08	289E+08	505E+08	207E+08	452E+08
Fumaric acid_C13-label-1	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	865E+07	962E+07	181E+08	968E+07	136E+08	160E+08	159E+08	152E+08	119E+08	149E+08	121E+08	117E+08	000E+00	160E+07	290E+07	129E+07	204E+07	247E+07	176E+07	224E+07	165E+07	260E+07	172E+07	281E+07
Fumaric acid_C13-label-2	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	113E+07	103E+07	248E+07	119E+07	176E+07	258E+07	234E+07	276E+07	987E+06	107E+07	124E+06	241E+06	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00
Fumaric acid_C13-label-3	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	793E+06	968E+06	180E+07	107E+07	123E+07	198E+07	197E+07	226E+07	659E+06	955E+06	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00
Fumaric acid_C13-label-4	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00
L-Malic acid_PARENT	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	603E+09	636E+09	485E+09	537E+09	563E+09	584E+09	701E+09	544E+09	598E+09	955E+09	795E+09	360E+09	196E+08	864E+08	224E+09	140E+09	448E+09	392E+09	187E+09	302E+09	164E+09	237E+09	758E+09	317E+09
L-Malic acid_C13-label-1	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	642E+08	179E+09	567E+08	134E+09	682E+08	735E+08	183E+09	796E+08	184E+09	254E+09	343E+08	169E+08	278E+07	217E+08	384E+08	381E+08	612E+08	551E+08	363E+08	558E+08	409E+08	747E+08	346E+08	161E+08
L-Malic acid_C13-label-2	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	142E+08	724E+08	129E+08	511E+08	199E+08	217E+08	660E+08	285E+08	817E+08	103E+09	282E+06	344E+06	102E+07	853E+07	150E+08	164E+08	236E+08	192E+08	130E+08	264E+08	175E+08	327E+08	964E+06	745E+06
L-Malic acid_C13-label-3	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	347E+07	204E+08	312E+07	130E+08	528E+07	620E+07	183E+08	896E+07	269E+08	295E+08	000E+00	000E+00	208E+06	225E+07	261E+07	447E+07	417E+07	424E+07	320E+07	526E+07	430E+07	104E+08	000E+00	000E+00
L-Malic acid_C13-label-4	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	530E+06	284E+07	521E+06	187E+07	483E+06	669E+06	233E+07	776E+06	426E+07	445E+07	000E+00	000E+00	263E+05	391E+06	269E+06	715E+06	693E+06	618E+06	669E+06	541E+06	557E+06	170E+07	000E+00	000E+00
Oxoglutaric acid_PARENT	280E+08	296E+08	284E+08	308E+08	364E+08	296E+08	166E+08	320E+08	218E+08	142E+08	171E+08	217E+08	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	151E+08	179E+08	000E+00	415E+08	000E+00	000E+00	101E+08	000E+00	931E+07	245E+08	111E+08	000E+00
Oxoglutaric acid_C13-label-1	240E+07	283E+07	332E+07	671E+07	397E+07	336E+07	304E+07	372E+07	413E+07	266E+07	842E+06	124E+07	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	227E+07	424E+07	000E+00	108E+08	780E+06	975E+06	188E+07	000E+00	217E+07	666E+07	609E+06	000E+00
Oxoglutaric acid_C13-label-2	641E+06	106E+07	131E+07	470E+07	125E+07	124E+07	167E+07	135E+07	294E+07	176E+07	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	118E+07	258E+07	000E+00	680E+07	256E+06	304E+06	105E+07	000E+00	117E+07	428E+07	000E+00	000E+00
Oxoglutaric acid_C13-label-3	164E+06	332E+06	298E+06	137E+07	299E+06	307E+06	519E+06	369E+06	807E+06	637E+06	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	276E+06	729E+06	000E+00	185E+07	000E+00	000E+00	354E+06	000E+00	248E+06	135E+07	000E+00	000E+00
Oxoglutaric acid_C13-label-4	407E+05	678E+05	855E+05	373E+06	620E+05	735E+05	121E+06	819E+05	186E+06	159E+06	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	888E+05	170E+06	000E+00	467E+06	000E+00	000E+00	933E+05	000E+00	457E+05	357E+06	000E+00	000E+00
Oxoglutaric acid_C13-label-5	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00
Succinic acid_PARENT	726E+08	443E+08	205E+08	143E+09	196E+08	170E+08	379E+08	121E+08	797E+08	285E+08	127E+08	125E+08	638E+09	783E+09	629E+09	920E+09	718E+09	707E+09	103E+10	646E+09	904E+09	918E+09	868E+09	613E+09	705E+08	518E+08	172E+09	395E+08	118E+09	198E+09	601E+08	159E+09	969E+08	117E+09	119E+09	168E+09
Succinic acid_C13-label-1	542E+07	649E+07	214E+07	312E+08	166E+07	157E+07	668E+07	104E+07	160E+08	538E+07	533E+06	560E+06	701E+08	212E+09	742E+08	220E+09	904E+08	914E+08	243E+09	959E+08	255E+09	233E+09	363E+08	284E+08	105E+08	113E+08	290E+08	103E+08	149E+08	266E+08	102E+08	291E+08	239E+08	361E+08	478E+07	766E+07
Succinic acid_C13-label-2	133E+07	341E+07	751E+06	195E+08	515E+06	500E+06	371E+07	265E+06	104E+08	333E+07	000E+00	000E+00	175E+08	949E+08	188E+08	905E+08	291E+08	296E+08	100E+09	377E+08	129E+09	103E+09	000E+00	000E+00	434E+07	454E+07	121E+08	466E+07	639E+07	971E+07	363E+07	151E+08	107E+08	168E+08	000E+00	000E+00
Succinic acid_C13-label-3	152E+06	941E+06	109E+06	414E+07	700E+05	459E+05	646E+06	375E+05	200E+07	613E+06	000E+00	000E+00	430E+07	230E+08	448E+07	206E+08	832E+07	837E+07	241E+08	118E+08	366E+08	248E+08	000E+00	000E+00	784E+06	104E+07	196E+07	115E+07	888E+06	196E+07	739E+06	264E+07	278E+07	494E+07	000E+00	000E+00
Succinic acid_C13-label-4	437E+05	145E+06	282E+04	121E+07	000E+00	000E+00	135E+06	000E+00	498E+06	132E+06	000E+00	000E+00	515E+06	323E+07	628E+06	289E+07	513E+06	749E+06	328E+07	926E+06	609E+07	371E+07	000E+00	000E+00	836E+05	108E+06	121E+06	143E+06	720E+05	185E+06	886E+05	202E+06	261E+06	738E+06	000E+00	000E+00
Oxalacetic acid_PARENT	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	493E+06	165E+07	105E+07	522E+06	740E+06	500E+06	253E+07	707E+06	145E+07	269E+07	277E+06	433E+06	000E+00	000E+00	115E+06	165E+06	551E+06	572E+06	237E+06	305E+06	133E+06	317E+06	287E+06	324E+06
Oxalacetic acid_C13-label-1	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	458E+06	000E+00	000E+00	000E+00	000E+00	789E+06	000E+00	499E+06	799E+06	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	232E+05	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00
Oxalacetic acid_C13-label-2	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00
Oxalacetic acid_C13-label-3	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00
Oxalacetic acid_C13-label-4	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00
L-Alanine_PARENT	101E+08	161E+08	797E+07	188E+08	617E+07	668E+07	819E+07	504E+07	225E+08	903E+07	742E+07	117E+08	183E+10	155E+10	213E+10	201E+10	138E+10	189E+10	148E+10	137E+10	157E+10	157E+10	183E+10	222E+10	131E+10	187E+10	182E+10	176E+10	167E+10	196E+10	200E+10	186E+10	200E+10	193E+10	186E+10	222E+10
L-Alanine_C13-label-1	529E+06	152E+07	495E+06	187E+07	433E+06	446E+06	874E+06	376E+06	225E+07	605E+06	239E+06	386E+06	108E+09	177E+09	119E+09	210E+09	808E+08	145E+09	157E+09	984E+08	175E+09	127E+09	609E+08	784E+08	524E+08	889E+08	751E+08	817E+08	709E+08	855E+08	884E+08	779E+08	935E+08	841E+08	605E+08	743E+08
L-Alanine_C13-label-2	148E+06	834E+06	179E+06	102E+07	178E+06	182E+06	435E+06	183E+06	106E+07	298E+06	000E+00	000E+00	332E+08	901E+08	332E+08	102E+09	252E+08	614E+08	711E+08	429E+08	770E+08	575E+08	481E+06	302E+07	870E+07	221E+08	143E+08	176E+08	148E+08	194E+08	169E+08	164E+08	191E+08	180E+08	435E+06	122E+07
L-Alanine_C13-label-3	332E+06	130E+07	386E+06	176E+07	332E+06	378E+06	803E+06	391E+06	200E+07	672E+06	000E+00	000E+00	541E+08	106E+09	487E+08	144E+09	313E+08	664E+08	916E+08	587E+08	133E+09	899E+08	000E+00	000E+00	165E+08	408E+08	247E+08	368E+08	240E+08	316E+08	361E+08	388E+08	366E+08	396E+08	000E+00	000E+00
L-Asparagine_PARENT	322E+07	624E+07	428E+07	732E+06	287E+07	373E+07	289E+07	272E+07	107E+07	157E+07	399E+07	582E+07	716E+08	532E+08	632E+08	562E+08	714E+08	611E+08	881E+08	471E+08	350E+08	394E+08	491E+08	556E+08	156E+08	591E+08	385E+08	615E+08	221E+08	279E+08	665E+08	272E+08	711E+08	509E+08	739E+08	534E+08
L-Asparagine_C13-label-1	508E+05	229E+06	176E+06	117E+05	111E+06	159E+06	133E+06	119E+06	326E+05	447E+05	155E+06	232E+06	244E+07	177E+07	218E+07	191E+07	245E+07	193E+07	316E+07	153E+07	106E+07	116E+07	154E+07	182E+07	545E+06	213E+07	136E+07	221E+07	680E+06	940E+06	241E+07	940E+06	252E+07	176E+07	264E+07	173E+07
L-Asparagine_C13-label-2	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00
L-Asparagine_C13-label-3	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00
L-Asparagine_C13-label-4	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00
L-Glutamic acid_PARENT	337E+08	596E+08	176E+08	345E+08	123E+08	150E+08	165E+08	961E+07	354E+08	120E+08	193E+08	110E+08	108E+10	726E+09	573E+09	602E+09	119E+10	108E+10	134E+10	122E+10	146E+10	587E+09	195E+10	752E+09	728E+08	137E+09	675E+08	126E+09	163E+09	102E+09	232E+09	136E+09	950E+08	116E+09	196E+09	156E+09
L-Glutamic acid_C13-label-1	281E+07	572E+07	185E+07	756E+07	113E+07	152E+07	289E+07	870E+06	693E+07	204E+07	986E+06	615E+06	106E+09	155E+09	573E+08	119E+09	125E+09	115E+09	262E+09	142E+09	325E+09	117E+09	110E+09	448E+08	108E+08	312E+08	108E+08	312E+08	242E+08	160E+08	429E+08	237E+08	219E+08	299E+08	107E+08	930E+07
L-Glutamic acid_C13-label-2	793E+06	214E+07	681E+06	524E+07	278E+06	537E+06	157E+07	247E+06	488E+07	132E+07	000E+00	469E+04	283E+08	778E+08	159E+08	596E+08	365E+08	341E+08	122E+09	508E+08	212E+09	617E+08	132E+07	176E+07	547E+07	189E+08	521E+07	198E+08	110E+08	699E+07	243E+08	143E+08	124E+08	189E+08	182E+06	397E+06
L-Glutamic acid_C13-label-3	203E+06	708E+06	162E+06	159E+07	550E+05	114E+06	487E+06	489E+05	135E+07	453E+06	000E+00	000E+00	788E+07	251E+08	433E+07	180E+08	110E+08	100E+08	382E+08	158E+08	670E+08	214E+08	000E+00	000E+00	124E+07	563E+07	904E+06	563E+07	248E+07	157E+07	845E+07	244E+07	274E+07	615E+07	000E+00	000E+00
L-Glutamic acid_C13-label-4	484E+05	157E+06	215E+05	403E+06	000E+00	885E+04	943E+05	406E+04	286E+06	104E+06	000E+00	000E+00	205E+07	304E+07	122E+07	246E+07	200E+07	211E+07	448E+07	290E+07	920E+07	260E+07	000E+00	000E+00	333E+06	137E+07	166E+06	137E+07	501E+06	307E+06	238E+07	510E+06	521E+06	148E+07	000E+00	000E+00
L-Glutamic acid_C13-label-5	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	129E+07	952E+06	673E+06	692E+06	983E+06	111E+07	155E+07	159E+07	223E+07	827E+06	000E+00	000E+00	134E+06	480E+06	177E+05	430E+06	245E+06	165E+06	951E+06	118E+06	476E+05	512E+06	000E+00	000E+00
L-Glutamine_PARENT	512E+08	608E+08	825E+08	400E+08	830E+08	818E+08	640E+08	752E+08	621E+08	426E+08	114E+09	111E+09	226E+10	156E+10	269E+10	178E+10	306E+10	251E+10	263E+10	293E+10	138E+10	168E+10	408E+10	385E+10	472E+09	108E+10	861E+09	116E+10	690E+09	747E+09	986E+09	916E+09	114E+10	112E+10	175E+10	142E+10
L-Glutamine_C13-label-1	550E+07	922E+07	992E+07	762E+07	954E+07	101E+08	128E+08	928E+07	114E+08	770E+07	604E+07	644E+07	220E+09	311E+09	274E+09	334E+09	322E+09	267E+09	495E+09	331E+09	271E+09	319E+09	227E+09	231E+09	594E+08	191E+09	112E+09	193E+09	855E+08	991E+08	175E+09	126E+09	168E+09	152E+09	976E+08	875E+08
L-Glutamine_C13-label-2	191E+07	512E+07	378E+07	479E+07	334E+07	379E+07	737E+07	356E+07	787E+07	493E+07	000E+00	268E+06	647E+08	169E+09	838E+08	174E+09	973E+08	855E+08	254E+09	120E+09	180E+09	181E+09	257E+07	992E+07	260E+08	141E+09	502E+08	145E+09	354E+08	445E+08	121E+09	638E+08	122E+09	109E+09	999E+06	483E+07
L-Glutamine_C13-label-3	662E+06	213E+07	128E+07	190E+07	120E+07	133E+07	277E+07	134E+07	244E+07	202E+07	000E+00	000E+00	188E+08	599E+08	246E+08	594E+08	306E+08	263E+08	875E+08	407E+08	618E+08	673E+08	000E+00	119E+07	960E+07	675E+08	196E+08	672E+08	141E+08	179E+08	538E+08	269E+08	351E+08	587E+08	000E+00	627E+06
L-Glutamine_C13-label-4	228E+06	603E+06	469E+06	519E+06	390E+06	444E+06	758E+06	480E+06	537E+06	542E+06	000E+00	000E+00	578E+07	977E+07	784E+07	963E+07	753E+07	664E+07	153E+08	107E+08	892E+07	954E+07	000E+00	000E+00	377E+07	245E+08	754E+07	241E+08	481E+07	606E+07	186E+08	105E+08	808E+07	221E+08	000E+00	000E+00
L-Glutamine_C13-label-5	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	297E+07	353E+07	386E+07	311E+07	389E+07	312E+07	574E+07	616E+07	194E+07	338E+07	000E+00	000E+00	170E+07	109E+08	367E+07	103E+08	236E+07	306E+07	781E+07	570E+07	140E+07	106E+08	000E+00	000E+00
L-Aspartic acid_PARENT	254E+08	965E+08	100E+08	363E+08	477E+07	696E+07	117E+08	368E+07	321E+08	738E+07	861E+07	120E+08	466E+09	264E+09	260E+09	233E+09	442E+09	421E+09	398E+09	470E+09	443E+09	151E+09	673E+09	247E+09	151E+08	287E+08	156E+08	236E+08	948E+07	879E+07	483E+08	301E+08	291E+08	205E+08	145E+09	496E+08
L-Aspartic acid_C13-label-1	173E+07	673E+07	793E+06	572E+07	272E+06	484E+06	147E+07	222E+06	462E+07	986E+06	358E+06	450E+06	347E+08	455E+08	183E+08	359E+08	348E+08	339E+08	675E+08	487E+08	996E+08	187E+08	292E+08	106E+08	170E+07	452E+07	209E+07	356E+07	921E+06	890E+06	660E+07	459E+07	573E+07	311E+07	605E+07	217E+07
L-Aspartic acid_C13-label-2	285E+06	155E+07	141E+06	278E+07	171E+05	824E+05	504E+06	177E+05	205E+07	309E+06	000E+00	000E+00	553E+07	155E+08	261E+07	117E+08	671E+07	648E+07	209E+08	133E+08	405E+08	548E+07	386E+06	272E+06	459E+06	143E+07	716E+06	118E+07	281E+06	256E+06	183E+07	192E+07	219E+07	102E+07	000E+00	000E+00
L-Aspartic acid_C13-label-3	830E+05	613E+06	455E+05	767E+06	111E+05	202E+05	188E+06	106E+05	734E+06	123E+06	000E+00	000E+00	185E+07	467E+07	943E+06	298E+07	225E+07	244E+07	656E+07	498E+07	129E+08	178E+07	000E+00	000E+00	767E+05	349E+06	111E+06	253E+06	753E+04	863E+04	485E+06	370E+06	425E+06	248E+06	000E+00	000E+00
L-Aspartic acid_C13-label-4	000E+00	832E+05	000E+00	171E+06	000E+00	000E+00	000E+00	000E+00	125E+06	195E+05	000E+00	000E+00	242E+06	574E+06	524E+05	310E+06	193E+06	145E+06	723E+06	337E+06	180E+07	208E+06	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00	000E+00
MS_METABOLITE_DATA_END
#METABOLITES
METABOLITES_START
metabolite_name
L-Lactic acid_PARENT
L-Lactic acid_C13-label-1
L-Lactic acid_C13-label-2
L-Lactic acid_C13-label-3
Pyruvic acid_PARENT
Pyruvic acid_C13-label-1
Pyruvic acid_C13-label-2
Pyruvic acid_C13-label-3
Citric acid_PARENT
Citric acid_C13-label-1
Citric acid_C13-label-2
Citric acid_C13-label-3
Citric acid_C13-label-4
Citric acid_C13-label-5
Citric acid_C13-label-6
Fumaric acid_PARENT
Fumaric acid_C13-label-1
Fumaric acid_C13-label-2
Fumaric acid_C13-label-3
Fumaric acid_C13-label-4
L-Malic acid_PARENT
L-Malic acid_C13-label-1
L-Malic acid_C13-label-2
L-Malic acid_C13-label-3
L-Malic acid_C13-label-4
Oxoglutaric acid_PARENT
Oxoglutaric acid_C13-label-1
Oxoglutaric acid_C13-label-2
Oxoglutaric acid_C13-label-3
Oxoglutaric acid_C13-label-4
Oxoglutaric acid_C13-label-5
Succinic acid_PARENT
Succinic acid_C13-label-1
Succinic acid_C13-label-2
Succinic acid_C13-label-3
Succinic acid_C13-label-4
Oxalacetic acid_PARENT
Oxalacetic acid_C13-label-1
Oxalacetic acid_C13-label-2
Oxalacetic acid_C13-label-3
Oxalacetic acid_C13-label-4
L-Alanine_PARENT
L-Alanine_C13-label-1
L-Alanine_C13-label-2
L-Alanine_C13-label-3
L-Asparagine_PARENT
L-Asparagine_C13-label-1
L-Asparagine_C13-label-2
L-Asparagine_C13-label-3
L-Asparagine_C13-label-4
L-Glutamic acid_PARENT
L-Glutamic acid_C13-label-1
L-Glutamic acid_C13-label-2
L-Glutamic acid_C13-label-3
L-Glutamic acid_C13-label-4
L-Glutamic acid_C13-label-5
L-Glutamine_PARENT
L-Glutamine_C13-label-1
L-Glutamine_C13-label-2
L-Glutamine_C13-label-3
L-Glutamine_C13-label-4
L-Glutamine_C13-label-5
L-Aspartic acid_PARENT
L-Aspartic acid_C13-label-1
L-Aspartic acid_C13-label-2
L-Aspartic acid_C13-label-3
L-Aspartic acid_C13-label-4
METABOLITES_END
#END