#METABOLOMICS WORKBENCH purdylab_20250622_094016 DATATRACK_ID:6075 STUDY_ID:ST004012 ANALYSIS_ID:AN006617 PROJECT_ID:PR002512 VERSION 1 CREATED_ON June 30, 2025, 6:33 pm #PROJECT PR:PROJECT_TITLE Measuring PC lipids in HCMV lytic infection. PR:PROJECT_SUMMARY Human cytomegalovirus (HCMV) is a common herpesvirus that establishes a lifelong PR:PROJECT_SUMMARY and persistent infection in its human host. HCMV infection in most people does PR:PROJECT_SUMMARY not cause overt disease. However, in immunocompromised individuals, severe PR:PROJECT_SUMMARY CMV-associated disease can lead to permanent disabilities and even death. PR:PROJECT_SUMMARY Additionally, congenital CMV is the leading infectious cause of birth defects. PR:PROJECT_SUMMARY Viruses have evolved to hijack host metabolic pathways to facilitate their PR:PROJECT_SUMMARY replication cycle. We previously reported HCMV infection increases PR:PROJECT_SUMMARY phosphatidylcholine (PC) lipid levels, including PCs with VLCFAs. To expand upon PR:PROJECT_SUMMARY the previously reported PC phenotype in HCMV infection, we determined the PC PR:PROJECT_SUMMARY lipidome of several infected cell types grown under various growth conditions. PR:PROJECT_SUMMARY Additionally, we determined which host pathways HCMV reprograms to induce PC PR:PROJECT_SUMMARY lipid synthesis and describe when during infection PC lipids changes occur. PR:INSTITUTE University of Arizona PR:DEPARTMENT Immunobiology PR:LABORATORY John G. Purdy, PhD PR:LAST_NAME Kline PR:FIRST_NAME Ian PR:ADDRESS 1657 E Helen St, Tucson, AZ 85721 PR:EMAIL ikline@arizona.edu PR:PHONE 5209092596 PR:FUNDING_SOURCE National Institute of Health (NIH) National Institute of Allergy and Infectious PR:FUNDING_SOURCE Disease (NIAID) R01AI162671, R01AI155539, F32AI178919, and National Institute of PR:FUNDING_SOURCE Aging (NIA) T32AG058503 award. #STUDY ST:STUDY_TITLE Measuring PC lipids in HCMV lytic infection. ST:STUDY_TYPE Experimental design: isotopic MS labeling and MS/MS abundance data. ST:STUDY_SUMMARY The levels of PC lipids are reported to increase in HCMV-infected cells. These ST:STUDY_SUMMARY studies were performed using growth conditions previously used in the HCMV field ST:STUDY_SUMMARY but are limited in their conclusions regarding our understanding of host and ST:STUDY_SUMMARY virus factors that influence the PC lipid phenotype observed in HCMV infection. ST:STUDY_SUMMARY Therefore, we tested several growth conditions including, cell type confluency, ST:STUDY_SUMMARY presence of serum, MOI, etc., that could impact the increase in PC lipids ST:STUDY_SUMMARY observed in HCMV infection. We find that HCMV promotes the levels of most PC ST:STUDY_SUMMARY lipids, including PC-VLCFAs. ST:INSTITUTE University of Arizona ST:DEPARTMENT Immunobiology ST:LABORATORY John G. Purdy, PhD ST:LAST_NAME Kline ST:FIRST_NAME Ian ST:ADDRESS 1657 E Helen St, Tucson, AZ 85721 ST:EMAIL ikline@arizona.edu ST:PHONE 5209092596 #SUBJECT SU:SUBJECT_TYPE Cultured cells SU:SUBJECT_SPECIES Homo sapiens SU:TAXONOMY_ID 9606 SU:GENDER Male and female SU:CELL_STRAIN_DETAILS HFF, MRC5, MRC9, ARPE19 SU:CELL_PASSAGE_NUMBER <30 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data SUBJECT_SAMPLE_FACTORS rep_1_heatmap_pos_HFF_uninfected_72_a 20190315_pos_HFF_mock_a Sample source:Primary human fibroblast cells (HFF) | Infection status:Uninfected Time (hpi)=72; RAW_FILE_NAME(.mzxML file name)=20190315_pos_HFF_mock_a SUBJECT_SAMPLE_FACTORS rep_1_heatmap_pos_HFF_uninfected_72_b 20190315_pos_HFF_mock_b Sample source:Primary human fibroblast cells (HFF) | Infection status:Uninfected Time (hpi)=72; RAW_FILE_NAME(.mzxML file name)=20190315_pos_HFF_mock_b SUBJECT_SAMPLE_FACTORS rep_1_heatmap_pos_HFF_HCMV_72_a 20190315_pos_HFF_TB40e_a Sample source:Primary human fibroblast cells (HFF) | Infection status:HCMV Time (hpi)=72; RAW_FILE_NAME(.mzxML file name)=20190315_pos_HFF_TB40e_a SUBJECT_SAMPLE_FACTORS rep_1_heatmap_pos_HFF_HCMV_72_b 20190315_pos_HFF_TB40e_b Sample source:Primary human fibroblast cells (HFF) | Infection status:HCMV Time (hpi)=72; RAW_FILE_NAME(.mzxML file name)=20190315_pos_HFF_TB40e_b SUBJECT_SAMPLE_FACTORS rep_1_heatmap_pos_MRC5_uninfected_72_a 20190315_pos_MRC5_mock_a Sample source:Primary human fibroblast cells (MRC5) | Infection status:Uninfected Time (hpi)=72; RAW_FILE_NAME(.mzxML file name)=20190315_pos_MRC5_mock_a SUBJECT_SAMPLE_FACTORS rep_1_heatmap_pos_MRC5_uninfected_72_b 20190315_pos_MRC5_mock_b Sample source:Primary human fibroblast cells (MRC5) | Infection status:Uninfected Time (hpi)=72; RAW_FILE_NAME(.mzxML file name)=20190315_pos_MRC5_mock_b SUBJECT_SAMPLE_FACTORS rep_1_heatmap_pos_MRC5_HCMV_72_a 20190315_pos_MRC5_TB40e_a Sample source:Primary human fibroblast cells (MRC5) | Infection status:HCMV Time (hpi)=72; RAW_FILE_NAME(.mzxML file name)=20190315_pos_MRC5_TB40e_a SUBJECT_SAMPLE_FACTORS rep_1_heatmap_pos_MRC5_HCMV_72_b 20190315_pos_MRC5_TB40e_b Sample source:Primary human fibroblast cells (MRC5) | Infection status:HCMV Time (hpi)=72; RAW_FILE_NAME(.mzxML file name)=20190315_pos_MRC5_TB40e_b SUBJECT_SAMPLE_FACTORS rep_1_heatmap_pos_MRC9_uninfected_72_a 20190315_pos_MRC9_mock_a Sample source:Primary human fibroblast cells (MRC9) | Infection status:Uninfected Time (hpi)=72; RAW_FILE_NAME(.mzxML file name)=20190315_pos_MRC9_mock_a SUBJECT_SAMPLE_FACTORS rep_1_heatmap_pos_MRC9_uninfected_72_b 20190315_pos_MRC9_mock_b Sample source:Primary human fibroblast cells (MRC9) | Infection status:Uninfected Time (hpi)=72; RAW_FILE_NAME(.mzxML file name)=20190315_pos_MRC9_mock_b SUBJECT_SAMPLE_FACTORS rep_1_heatmap_pos_MRC9_HCMV_72_a 20190315_pos_MRC9_TB40e_a Sample source:Primary human fibroblast cells (MRC9) | Infection status:HCMV Time (hpi)=72; RAW_FILE_NAME(.mzxML file name)=20190315_pos_MRC9_TB40e_a SUBJECT_SAMPLE_FACTORS rep_1_heatmap_pos_MRC9_HCMV_72_b 20190315_pos_MRC9_TB40e_b Sample source:Primary human fibroblast cells (MRC9) | Infection status:HCMV Time (hpi)=72; RAW_FILE_NAME(.mzxML file name)=20190315_pos_MRC9_TB40e_b SUBJECT_SAMPLE_FACTORS rep_1_heatmap_pos_ARPE19_uninfected_72_a 20180908_pos_1_mock_arpe-19 Sample source:Primary human epithelial cells (ARPE19) | Infection status:Unifected Time (hpi)=72; RAW_FILE_NAME(.mzxML file name)=20180908_pos_1_mock_arpe-19 SUBJECT_SAMPLE_FACTORS rep_1_heatmap_pos_ARPE19_uninfected_72_b 20180908_pos_2_mock_arpe-19 Sample source:Primary human epithelial cells (ARPE19) | Infection status:Unifected Time (hpi)=72; RAW_FILE_NAME(.mzxML file name)=20180908_pos_2_mock_arpe-19 SUBJECT_SAMPLE_FACTORS rep_1_heatmap_pos_ARPE19_HCMVfibro_72_a 20180908_pos_3_tb40Efibro_arpe-19 Sample source:Primary human epithelial cells (ARPE19) | Infection status:HCMV Time (hpi)=72; RAW_FILE_NAME(.mzxML file name)=20180908_pos_3_tb40Efibro_arpe-19 SUBJECT_SAMPLE_FACTORS rep_1_heatmap_pos_ARPE19_HCMVfibro_72_b 20180908_pos_4_tb40Efibro_arpe-19 Sample source:Primary human epithelial cells (ARPE19) | Infection status:HCMV Time (hpi)=72; RAW_FILE_NAME(.mzxML file name)=20180908_pos_4_tb40Efibro_arpe-19 SUBJECT_SAMPLE_FACTORS rep_1_heatmap_pos_ARPE19_HCMVepi_72_a 20180908_pos_5_tb40Eepi_arpe-19 Sample source:Primary human epithelial cells (ARPE19) | Infection status:HCMV Time (hpi)=72; RAW_FILE_NAME(.mzxML file name)=20180908_pos_5_tb40Eepi_arpe-19 SUBJECT_SAMPLE_FACTORS rep_1_heatmap_pos_ARPE19_HCMVepi_72_b 20180908_pos_6_tb40Eepi_arpe-19 Sample source:Primary human epithelial cells (ARPE19) | Infection status:HCMV Time (hpi)=72; RAW_FILE_NAME(.mzxML file name)=20180908_pos_6_tb40Eepi_arpe-19 SUBJECT_SAMPLE_FACTORS rep_1_20190320_pos_endo_72_mock_a 20190320_pos_1_mock Sample source:Primary human endothelial cells (HUVEC) | Infection status:Unifected Time (hpi)=72; RAW_FILE_NAME(.mzxML file name)=20190320_pos_1_mock SUBJECT_SAMPLE_FACTORS rep_1_20190320_pos_endo_72_mock_a 20190320_pos_2_mock Sample source:Primary human endothelial cells (HUVEC) | Infection status:Unifected Time (hpi)=72; RAW_FILE_NAME(.mzxML file name)=20190320_pos_2_mock SUBJECT_SAMPLE_FACTORS rep_1_20190320_pos_endo_72_TB40E_a 20190320_pos_3_wt_UL136myc Sample source:Primary human endothelial cells (HUVEC) | Infection status:HCMV Time (hpi)=72; RAW_FILE_NAME(.mzxML file name)=20190320_pos_3_wt_UL136myc SUBJECT_SAMPLE_FACTORS rep_1_20190320_pos_endo_72_TB40E_b 20190320_pos_4_wt_UL136myc Sample source:Primary human endothelial cells (HUVEC) | Infection status:HCMV Time (hpi)=72; RAW_FILE_NAME(.mzxML file name)=20190320_pos_4_wt_UL136myc SUBJECT_SAMPLE_FACTORS rep_2_20190813_pos_endo_72_mock_2_a 20190813_pos_endo_m_rep2_a Sample source:Primary human endothelial cells (HUVEC) | Infection status:Unifected Time (hpi)=72; RAW_FILE_NAME(.mzxML file name)=20190813_pos_endo_m_rep2_a SUBJECT_SAMPLE_FACTORS rep_2_20190813_pos_endo_72_mock_2_b 20190813_pos_endo_m_rep2_b Sample source:Primary human endothelial cells (HUVEC) | Infection status:Unifected Time (hpi)=72; RAW_FILE_NAME(.mzxML file name)=20190813_pos_endo_m_rep2_b SUBJECT_SAMPLE_FACTORS rep_2_20190813_pos_endo_72_TB40E_2_a 20190813_pos_endo_tb40e_rep2_a Sample source:Primary human endothelial cells (HUVEC) | Infection status:HCMV Time (hpi)=72; RAW_FILE_NAME(.mzxML file name)=20190813_pos_endo_tb40e_rep2_a SUBJECT_SAMPLE_FACTORS rep_2_20190813_pos_endo_72_TB40E_2_b 20190813_pos_endo_tb40e_rep2_b Sample source:Primary human endothelial cells (HUVEC) | Infection status:HCMV Time (hpi)=72; RAW_FILE_NAME(.mzxML file name)=20190813_pos_endo_tb40e_rep2_b SUBJECT_SAMPLE_FACTORS rep_3_20190813_pos_endo_72_mock_3_a 20190813_pos_endo_m_rep3_a Sample source:Primary human endothelial cells (HUVEC) | Infection status:Unifected Time (hpi)=72; RAW_FILE_NAME(.mzxML file name)=20190813_pos_endo_m_rep3_a SUBJECT_SAMPLE_FACTORS rep_3_20190813_pos_endo_72_mock_3_b 20190813_pos_endo_m_rep3_b Sample source:Primary human endothelial cells (HUVEC) | Infection status:Unifected Time (hpi)=72; RAW_FILE_NAME(.mzxML file name)=20190813_pos_endo_m_rep3_b SUBJECT_SAMPLE_FACTORS rep_3_20190813_pos_endo_72_TB40E_3_a 20190813_pos_endo_tb40e_rep3_a Sample source:Primary human endothelial cells (HUVEC) | Infection status:HCMV Time (hpi)=72; RAW_FILE_NAME(.mzxML file name)=20190813_pos_endo_tb40e_rep3_a SUBJECT_SAMPLE_FACTORS rep_3_20190813_pos_endo_72_TB40E_3_b 20190813_pos_endo_tb40e_rep3_b Sample source:Primary human endothelial cells (HUVEC) | Infection status:HCMV Time (hpi)=72; RAW_FILE_NAME(.mzxML file name)=20190813_pos_endo_tb40e_rep3_b SUBJECT_SAMPLE_FACTORS rep_1_heatmap_pos_HFF_uninfected_IK_72_a 20230603_IK_pos_mock_choline_72_a Sample source:Primary human fibroblast cells (HFF) | Infection status:Uninfected Time (hpi)=72; RAW_FILE_NAME(.mzxML file name)=20230603_IK_pos_mock_choline_72_a SUBJECT_SAMPLE_FACTORS rep_1_heatmap_pos_HFF_uninfected_IK_72_b 20230603_IK_pos_mock_choline_72_b Sample source:Primary human fibroblast cells (HFF) | Infection status:Uninfected Time (hpi)=72; RAW_FILE_NAME(.mzxML file name)=20230603_IK_pos_mock_choline_72_b SUBJECT_SAMPLE_FACTORS rep_1_heatmap_pos_HFF_HCMV_IK_72_a 20230603_IK_pos_TB40E_choline_72_a Sample source:Primary human fibroblast cells (HFF) | Infection status:HCMV Time (hpi)=72; RAW_FILE_NAME(.mzxML file name)=20230603_IK_pos_TB40E_choline_72_a SUBJECT_SAMPLE_FACTORS rep_1_heatmap_pos_HFF_HCMV_IK_72_b 20230603_IK_pos_TB40E_choline_72_b Sample source:Primary human fibroblast cells (HFF) | Infection status:HCMV Time (hpi)=72; RAW_FILE_NAME(.mzxML file name)=20230603_IK_pos_TB40E_choline_72_b SUBJECT_SAMPLE_FACTORS rep_1_heatmap_pos_HFF_uninfected_RM_72_a 20230603_RM_pos_mock_choline_72_a Sample source:Primary human fibroblast cells (HFF) | Infection status:Uninfected Time (hpi)=72; RAW_FILE_NAME(.mzxML file name)=20230603_RM_pos_mock_choline_72_a SUBJECT_SAMPLE_FACTORS rep_1_heatmap_pos_HFF_uninfected_RM_72_b 20230603_RM_pos_mock_choline_72_b Sample source:Primary human fibroblast cells (HFF) | Infection status:Uninfected Time (hpi)=72; RAW_FILE_NAME(.mzxML file name)=20230603_RM_pos_mock_choline_72_b SUBJECT_SAMPLE_FACTORS rep_1_heatmap_pos_HFF_HCMV_RM_72_a 20230603_RM_pos_TB40E_choline_72_a Sample source:Primary human fibroblast cells (HFF) | Infection status:HCMV Time (hpi)=72; RAW_FILE_NAME(.mzxML file name)=20230603_RM_pos_TB40E_choline_72_a SUBJECT_SAMPLE_FACTORS rep_1_heatmap_pos_HFF_HCMV_RM_72_b 20230603_RM_pos_TB40E_choline_72_b Sample source:Primary human fibroblast cells (HFF) | Infection status:HCMV Time (hpi)=72; RAW_FILE_NAME(.mzxML file name)=20230603_RM_pos_TB40E_choline_72_b SUBJECT_SAMPLE_FACTORS rep_2_heatmap_pos_HFF_uninfected_IK_72_a 20230606_IK_pos_mock_choline_72_a Sample source:Primary human fibroblast cells (HFF) | Infection status:Uninfected Time (hpi)=72; RAW_FILE_NAME(.mzxML file name)=20230606_IK_pos_mock_choline_72_a SUBJECT_SAMPLE_FACTORS rep_2_heatmap_pos_HFF_uninfected_IK_72_b 20230606_IK_pos_mock_choline_72_b Sample source:Primary human fibroblast cells (HFF) | Infection status:Uninfected Time (hpi)=72; RAW_FILE_NAME(.mzxML file name)=20230606_IK_pos_mock_choline_72_b SUBJECT_SAMPLE_FACTORS rep_2_heatmap_pos_HFF_HCMV_IK_72_a 20230606_IK_pos_TB40E_choline_72_a Sample source:Primary human fibroblast cells (HFF) | Infection status:HCMV Time (hpi)=72; RAW_FILE_NAME(.mzxML file name)=20230606_IK_pos_TB40E_choline_72_a SUBJECT_SAMPLE_FACTORS rep_2_heatmap_pos_HFF_HCMV_IK_72_b 20230606_IK_pos_TB40E_choline_72_b Sample source:Primary human fibroblast cells (HFF) | Infection status:HCMV Time (hpi)=72; RAW_FILE_NAME(.mzxML file name)=20230606_IK_pos_TB40E_choline_72_b SUBJECT_SAMPLE_FACTORS rep_2_heatmap_pos_HFF_uninfected_RM_72_a 20230606_RM_pos_mock_choline_72_a Sample source:Primary human fibroblast cells (HFF) | Infection status:Uninfected Time (hpi)=72; RAW_FILE_NAME(.mzxML file name)=20230606_RM_pos_mock_choline_72_a SUBJECT_SAMPLE_FACTORS rep_2_heatmap_pos_HFF_uninfected_RM_72_b 20230606_RM_pos_mock_choline_72_b Sample source:Primary human fibroblast cells (HFF) | Infection status:Uninfected Time (hpi)=72; RAW_FILE_NAME(.mzxML file name)=20230606_RM_pos_mock_choline_72_b SUBJECT_SAMPLE_FACTORS rep_2_heatmap_pos_HFF_HCMV_RM_72_a 20230606_RM_pos_TB40E_choline_72_a Sample source:Primary human fibroblast cells (HFF) | Infection status:HCMV Time (hpi)=72; RAW_FILE_NAME(.mzxML file name)=20230606_RM_pos_TB40E_choline_72_a SUBJECT_SAMPLE_FACTORS rep_2_heatmap_pos_HFF_HCMV_RM_72_b 20230606_RM_pos_TB40E_choline_72_b Sample source:Primary human fibroblast cells (HFF) | Infection status:HCMV Time (hpi)=72; RAW_FILE_NAME(.mzxML file name)=20230606_RM_pos_TB40E_choline_72_b SUBJECT_SAMPLE_FACTORS rep_3_heatmap_pos_HFF_uninfected_IK_72_a 20230610_IK_pos_mock_choline_72_a Sample source:Primary human fibroblast cells (HFF) | Infection status:Uninfected Time (hpi)=72; RAW_FILE_NAME(.mzxML file name)=20230610_IK_pos_mock_choline_72_a SUBJECT_SAMPLE_FACTORS rep_3_heatmap_pos_HFF_uninfected_IK_72_b 20230610_IK_pos_mock_choline_72_b Sample source:Primary human fibroblast cells (HFF) | Infection status:Uninfected Time (hpi)=72; RAW_FILE_NAME(.mzxML file name)=20230610_IK_pos_mock_choline_72_b SUBJECT_SAMPLE_FACTORS rep_3_heatmap_pos_HFF_HCMV_IK_72_a 20230610_IK_pos_TB40E_choline_72_a Sample source:Primary human fibroblast cells (HFF) | Infection status:HCMV Time (hpi)=72; RAW_FILE_NAME(.mzxML file name)=20230610_IK_pos_TB40E_choline_72_a SUBJECT_SAMPLE_FACTORS rep_3_heatmap_pos_HFF_HCMV_IK_72_b 20230610_IK_pos_TB40E_choline_72_b Sample source:Primary human fibroblast cells (HFF) | Infection status:HCMV Time (hpi)=72; RAW_FILE_NAME(.mzxML file name)=20230610_IK_pos_TB40E_choline_72_b SUBJECT_SAMPLE_FACTORS rep_3_heatmap_pos_HFF_uninfected_RM_72_a 20230610_RM_pos_mock_choline_72_a Sample source:Primary human fibroblast cells (HFF) | Infection status:Uninfected Time (hpi)=72; RAW_FILE_NAME(.mzxML file name)=20230610_RM_pos_mock_choline_72_a SUBJECT_SAMPLE_FACTORS rep_3_heatmap_pos_HFF_uninfected_RM_72_b 20230610_RM_pos_mock_choline_72_b Sample source:Primary human fibroblast cells (HFF) | Infection status:Uninfected Time (hpi)=72; RAW_FILE_NAME(.mzxML file name)=20230610_RM_pos_mock_choline_72_b SUBJECT_SAMPLE_FACTORS rep_3_heatmap_pos_HFF_HCMV_RM_72_a 20230610_RM_pos_TB40E_choline_72_a Sample source:Primary human fibroblast cells (HFF) | Infection status:HCMV Time (hpi)=72; RAW_FILE_NAME(.mzxML file name)=20230610_RM_pos_TB40E_choline_72_a SUBJECT_SAMPLE_FACTORS rep_3_heatmap_pos_HFF_HCMV_RM_72_b 20230610_RM_pos_TB40E_choline_72_b Sample source:Primary human fibroblast cells (HFF) | Infection status:HCMV Time (hpi)=72; RAW_FILE_NAME(.mzxML file name)=20230610_RM_pos_TB40E_choline_72_b SUBJECT_SAMPLE_FACTORS rep_1_timecourse_pos_uninfected_72_a 20191114_pos_mock_NT_72hpi_a Sample source:Primary human fibroblast cells (HFF) | Infection status:Uninfected Time (hpi)=72; RAW_FILE_NAME(.mzxML file name)=20191114_pos_mock_NT_72hpi_a SUBJECT_SAMPLE_FACTORS rep_1_timecourse_pos_uninfected_72_b 20191114_pos_mock_NT_72hpi_b Sample source:Primary human fibroblast cells (HFF) | Infection status:Uninfected Time (hpi)=72; RAW_FILE_NAME(.mzxML file name)=20191114_pos_mock_NT_72hpi_b SUBJECT_SAMPLE_FACTORS rep_1_timecourse_pos_HCMV_72_a 20191114_pos_TB40E_NT_72hpi_a Sample source:Primary human fibroblast cells (HFF) | Infection status:HCMV Time (hpi)=72; RAW_FILE_NAME(.mzxML file name)=20191114_pos_TB40E_NT_72hpi_a SUBJECT_SAMPLE_FACTORS rep_1_timecourse_pos_HCMV_72_b 20191114_pos_TB40E_NT_72hpi_b Sample source:Primary human fibroblast cells (HFF) | Infection status:HCMV Time (hpi)=72; RAW_FILE_NAME(.mzxML file name)=20191114_pos_TB40E_NT_72hpi_b SUBJECT_SAMPLE_FACTORS rep_2_timecourse_pos_uninfected_72_a 20191122_pos_mock_NT_72hpi_a Sample source:Primary human fibroblast cells (HFF) | Infection status:Uninfected Time (hpi)=72; RAW_FILE_NAME(.mzxML file name)=20191122_pos_mock_NT_72hpi_a SUBJECT_SAMPLE_FACTORS rep_2_timecourse_pos_uninfected_72_b 20191122_pos_mock_NT_72hpi_b Sample source:Primary human fibroblast cells (HFF) | Infection status:Uninfected Time (hpi)=72; RAW_FILE_NAME(.mzxML file name)=20191122_pos_mock_NT_72hpi_b SUBJECT_SAMPLE_FACTORS rep_2_timecourse_pos_HCMV_72_a 20191122_pos_TB40E_NT_72hpi_a Sample source:Primary human fibroblast cells (HFF) | Infection status:HCMV Time (hpi)=72; RAW_FILE_NAME(.mzxML file name)=20191122_pos_TB40E_NT_72hpi_a SUBJECT_SAMPLE_FACTORS rep_2_timecourse_pos_HCMV_72_b 20191122_pos_TB40E_NT_72hpi_b Sample source:Primary human fibroblast cells (HFF) | Infection status:HCMV Time (hpi)=72; RAW_FILE_NAME(.mzxML file name)=20191122_pos_TB40E_NT_72hpi_b SUBJECT_SAMPLE_FACTORS rep_3_timecourse_pos_uninfected_72_a 20191124_pos_mock_NT_72hpi_a Sample source:Primary human fibroblast cells (HFF) | Infection status:Uninfected Time (hpi)=72; RAW_FILE_NAME(.mzxML file name)=20191124_pos_mock_NT_72hpi_a SUBJECT_SAMPLE_FACTORS rep_3_timecourse_pos_uninfected_72_b 20191124_pos_mock_NT_72hpi_b Sample source:Primary human fibroblast cells (HFF) | Infection status:Uninfected Time (hpi)=72; RAW_FILE_NAME(.mzxML file name)=20191124_pos_mock_NT_72hpi_b SUBJECT_SAMPLE_FACTORS rep_3_timecourse_pos_HCMV_72_a 20191124_pos_TB40E_NT_72hpi_a Sample source:Primary human fibroblast cells (HFF) | Infection status:HCMV Time (hpi)=72; RAW_FILE_NAME(.mzxML file name)=20191124_pos_TB40E_NT_72hpi_a SUBJECT_SAMPLE_FACTORS rep_3_timecourse_pos_HCMV_72_b 20191124_pos_TB40E_NT_72hpi_b Sample source:Primary human fibroblast cells (HFF) | Infection status:HCMV Time (hpi)=72; RAW_FILE_NAME(.mzxML file name)=20191124_pos_TB40E_NT_72hpi_b #COLLECTION CO:COLLECTION_SUMMARY General Notes: -Work over ice when possible (during scraping and between vortex CO:COLLECTION_SUMMARY steps etc.). -Chloroform leeches plastics. Avoid contact with plastic caps, CO:COLLECTION_SUMMARY tubes and gloves around glass vial tops. -Work in manageable batch numbers. A CO:COLLECTION_SUMMARY batch of 8-16 samples at a time is common. -Clean syringes using chloroform CO:COLLECTION_SUMMARY before and after collection. Use a unique syringe for each sample (the same CO:COLLECTION_SUMMARY syringe can be used if A/B technical replicates are used. Recommend a quick CO:COLLECTION_SUMMARY flush of the syringe using 500 μL chloroform before moving from A to B). Begin by CO:COLLECTION_SUMMARY counting cell numbers for each sample using a dedicated well meant for MS CO:COLLECTION_SUMMARY normalization based on cell count. In a 6-well plate, wash cells 2x with cold CO:COLLECTION_SUMMARY PBS. Add 1mL of cold 50% methanol to each well and scrape cells into glass CO:COLLECTION_SUMMARY vials. Add 500 μL of chloroform. Vortex on low setting (careful to avoid CO:COLLECTION_SUMMARY splashing chloroform onto plastic caps and liners). Centrifuge @1000g for 5 CO:COLLECTION_SUMMARY mins. There should be a clear phase separation of methanol and cell debris on CO:COLLECTION_SUMMARY top, while the lower phase contains chloroform and lipids. Use a syringe to CO:COLLECTION_SUMMARY carefully extract the lower phase without transferring cell debris from the top CO:COLLECTION_SUMMARY layer. Transfer to clean vial and place on ice. Once all lipids have been CO:COLLECTION_SUMMARY extracted, add 500 μL of chloroform and repeat the process again once more. In CO:COLLECTION_SUMMARY total each sample should contain ~1 mL of chloroform and lipids from two CO:COLLECTION_SUMMARY extractions. Carefully, dry lipids under nitrogen gas. Avoid direct high CO:COLLECTION_SUMMARY pressure air flow onto chloroform:lipid solution as it can splash high up in CO:COLLECTION_SUMMARY vial walls. Store at -80℃ for up to 30 days. Samples may become unstable and CO:COLLECTION_SUMMARY degrade over time. CO:SAMPLE_TYPE Cultured cells CO:COLLECTION_LOCATION Fume hood required when working with chloroform. CO:COLLECTION_FREQUENCY Perform extraction twice on each sample. CO:COLLECTION_DURATION 1-2 hours dependent upon batch size CO:VOLUMEORAMOUNT_COLLECTED ~1 mL chloroform:lipid solution CO:STORAGE_CONDITIONS -80℃ CO:COLLECTION_VIALS Glass vials, no plastic! (plastic cap is okay) CO:STORAGE_VIALS Glass vials, no plastic! (plastic cap is okay) CO:COLLECTION_TUBE_TEMP 25 CO:ADDITIVES UHPLC 100% chloroform #TREATMENT TR:TREATMENT_SUMMARY Cells were grown to full confluence and held for 3-days in Dulbecco's modified TR:TREATMENT_SUMMARY eagle medium (DMEM) with 10% fetal bovine serum (FBS). 24 hours prior to TR:TREATMENT_SUMMARY infection, cells were starved of serum in DMEM lacking FBS. Cells were TR:TREATMENT_SUMMARY HCMV-infected or mock-infected in serum-free DMEM for 1 hour. 1 hpi, cells were TR:TREATMENT_SUMMARY maintained in serum-free growth medium. Medium was replaced at 48 hpi. #SAMPLEPREP SP:SAMPLEPREP_SUMMARY Before starting, calculate the volume of 1:1:1 choloroform:methanol:isopropanol SP:SAMPLEPREP_SUMMARY resuspension buffer needed for each sample. Volume of 1:1:1 is dependent on SP:SAMPLEPREP_SUMMARY normalize cell count from experiment. Use 200 μL of 1:1:1 for every 2E5 cells. SP:SAMPLEPREP_SUMMARY When ready to start resuspension, remove dried lipids from -80C storage and SP:SAMPLEPREP_SUMMARY resuspend in volume of 1:1:1 solution calculated for each sample. No cell SP:SAMPLEPREP_SUMMARY conditions use 200 μL. Use gentle vortex to allow for dried lipids from vial wall SP:SAMPLEPREP_SUMMARY to get into solution. Prepare "blank" vials of 1:1:1 for buffer background SP:SAMPLEPREP_SUMMARY analysis. Store lipids in autosampler between 4-7℃. #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE Reversed phase CH:INSTRUMENT_NAME Thermo Vanquish CH:COLUMN_NAME Phenomenex Kinetex C18 (100 x 2.1 mm, 2.6 μm) CH:SOLVENT_A 40:60 water-methanol, plus 10mM ammonium formate and 0.1% formic acid CH:SOLVENT_B 10:90 methanol-isopropanol, plus 10mM ammonium formate and 0.1% formic acid CH:FLOW_GRADIENT 75% solvent A–25% solvent B for 2 min, 35% solvent A–65% solvent B for 2min CH:FLOW_GRADIENT at a curve value of 4, a hold at 35% solvent A–65% solvent B for 1min, 0% CH:FLOW_GRADIENT solvent A–100% solvent B for 11min at a curve value of 4, and a hold at 0% CH:FLOW_GRADIENT solvent A–100% solvent B for 4 min. CH:FLOW_RATE 0.25 mL/min CH:COLUMN_TEMPERATURE 60℃ #ANALYSIS AN:ANALYSIS_TYPE MS #MS MS:INSTRUMENT_NAME Thermo Orbitrap Exploris 240 MS:INSTRUMENT_TYPE Orbitrap MS:MS_TYPE ESI MS:ION_MODE POSITIVE MS:MS_COMMENTS PCs were identified in positive mode using parent lipid m/z, RT and choline head MS:MS_COMMENTS fragment of 184.073 m/z. Confirmation of PCs was performed by FA tail analysis MS:MS_COMMENTS in negative mode. #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS Peak Area MS_METABOLITE_DATA_START Samples 20190315_pos_HFF_mock_a 20190315_pos_HFF_mock_b 20190315_pos_HFF_TB40e_a 20190315_pos_HFF_TB40e_b 20190315_pos_MRC5_mock_a 20190315_pos_MRC5_mock_b 20190315_pos_MRC5_TB40e_a 20190315_pos_MRC5_TB40e_b 20190315_pos_MRC9_mock_a 20190315_pos_MRC9_mock_b 20190315_pos_MRC9_TB40e_a 20190315_pos_MRC9_TB40e_b 20180908_pos_1_mock_arpe-19 20180908_pos_2_mock_arpe-19 20180908_pos_3_tb40Efibro_arpe-19 20180908_pos_4_tb40Efibro_arpe-19 20180908_pos_5_tb40Eepi_arpe-19 20180908_pos_6_tb40Eepi_arpe-19 20190320_pos_1_mock 20190320_pos_2_mock 20190320_pos_3_wt_UL136myc 20190320_pos_4_wt_UL136myc 20190813_pos_endo_m_rep2_b 20190813_pos_endo_m_rep2_b 20190813_pos_endo_tb40e_rep2_a 20190813_pos_endo_tb40e_rep2_b 20190813_pos_endo_m_rep3_a 20190813_pos_endo_m_rep3_b 20190813_pos_endo_tb40e_rep3_a 20190813_pos_endo_tb40e_rep3_b 20230603_IK_pos_mock_choline_72_a 20230603_IK_pos_mock_choline_72_b 20230603_IK_pos_TB40E_choline_72_a 20230603_IK_pos_TB40E_choline_72_b 20230603_RM_pos_mock_choline_72_a 20230603_RM_pos_mock_choline_72_b 20230603_RM_pos_TB40E_choline_72_a 20230603_RM_pos_TB40E_choline_72_b 20230606_IK_pos_mock_choline_72_a 20230606_IK_pos_mock_choline_72_b 20230606_IK_pos_TB40E_choline_72_a 20230606_IK_pos_TB40E_choline_72_b 20230606_RM_pos_mock_choline_72_a 20230606_RM_pos_mock_choline_72_b 20230606_RM_pos_TB40E_choline_72_a 20230606_RM_pos_TB40E_choline_72_b 20230610_IK_pos_mock_choline_72_a 20230610_IK_pos_mock_choline_72_b 20230610_IK_pos_TB40E_choline_72_a 20230610_IK_pos_TB40E_choline_72_b 20230610_RM_pos_mock_choline_72_a 20230610_RM_pos_mock_choline_72_b 20230610_RM_pos_TB40E_choline_72_a 20230610_RM_pos_TB40E_choline_72_b 20191114_pos_mock_NT_72hpi_a 20191114_pos_mock_NT_72hpi_b 20191114_pos_TB40E_NT_72hpi_a 20191114_pos_TB40E_NT_72hpi_b 20191122_pos_mock_NT_72hpi_a 20191122_pos_mock_NT_72hpi_b 20191122_pos_TB40E_NT_72hpi_a 20191122_pos_TB40E_NT_72hpi_b 20191124_pos_mock_NT_72hpi_a 20191124_pos_mock_NT_72hpi_b 20191124_pos_TB40E_NT_72hpi_a 20191124_pos_TB40E_NT_72hpi_b Factors Sample source:Primary human fibroblast cells (HFF) | Infection status:Uninfected Sample source:Primary human fibroblast cells (HFF) | Infection status:Uninfected Sample source:Primary human fibroblast cells (HFF) | Infection status:HCMV Sample source:Primary human fibroblast cells (HFF) | Infection status:HCMV Sample source:Primary human fibroblast cells (MRC5) | Infection status:Uninfected Sample source:Primary human fibroblast cells (MRC5) | Infection status:Uninfected Sample source:Primary human fibroblast cells (MRC5) | Infection status:HCMV Sample source:Primary human fibroblast cells (MRC5) | Infection status:HCMV Sample source:Primary human fibroblast cells (MRC9) | Infection status:Uninfected Sample source:Primary human fibroblast cells (MRC9) | Infection status:Uninfected Sample source:Primary human fibroblast cells (MRC9) | Infection status:HCMV Sample source:Primary human fibroblast cells (MRC9) | Infection status:HCMV Sample source:Primary human epithelial cells (ARPE19) | Infection status:Unifected Sample source:Primary human epithelial cells (ARPE19) | Infection status:Unifected Sample source:Primary human epithelial cells (ARPE19) | Infection status:HCMV Sample source:Primary human epithelial cells (ARPE19) | Infection status:HCMV Sample source:Primary human epithelial cells (ARPE19) | Infection status:HCMV Sample source:Primary human epithelial cells (ARPE19) | Infection status:HCMV Sample source:Primary human endothelial cells (HUVEC) | Infection status:Unifected Sample source:Primary human endothelial cells (HUVEC) | Infection status:Unifected Sample source:Primary human endothelial cells (HUVEC) | Infection status:HCMV Sample source:Primary human endothelial cells (HUVEC) | Infection status:HCMV Sample source:Primary human endothelial cells (HUVEC) | Infection status:Unifected Sample source:Primary human endothelial cells (HUVEC) | Infection status:Unifected Sample source:Primary human endothelial cells (HUVEC) | Infection status:HCMV Sample source:Primary human endothelial cells (HUVEC) | Infection status:HCMV Sample source:Primary human endothelial cells (HUVEC) | Infection status:Unifected Sample source:Primary human endothelial cells (HUVEC) | Infection status:Unifected Sample source:Primary human endothelial cells (HUVEC) | Infection status:HCMV Sample source:Primary human endothelial cells (HUVEC) | Infection status:HCMV Sample source:Primary human fibroblast cells (HFF) | Infection status:Uninfected Sample source:Primary human fibroblast cells (HFF) | Infection status:Uninfected Sample source:Primary human fibroblast cells (HFF) | Infection status:HCMV Sample source:Primary human fibroblast cells (HFF) | Infection status:HCMV Sample source:Primary human fibroblast cells (HFF) | Infection status:Uninfected Sample source:Primary human fibroblast cells (HFF) | Infection status:Uninfected Sample source:Primary human fibroblast cells (HFF) | Infection status:HCMV Sample source:Primary human fibroblast cells (HFF) | Infection status:HCMV Sample source:Primary human fibroblast cells (HFF) | Infection status:Uninfected Sample source:Primary human fibroblast cells (HFF) | Infection status:Uninfected Sample source:Primary human fibroblast cells (HFF) | Infection status:HCMV Sample source:Primary human fibroblast cells (HFF) | Infection status:HCMV Sample source:Primary human fibroblast cells (HFF) | Infection status:Uninfected Sample source:Primary human fibroblast cells (HFF) | Infection status:Uninfected Sample source:Primary human fibroblast cells (HFF) | Infection status:HCMV Sample source:Primary human fibroblast cells (HFF) | Infection status:HCMV Sample source:Primary human fibroblast cells (HFF) | Infection status:Uninfected Sample source:Primary human fibroblast cells (HFF) | Infection status:Uninfected Sample source:Primary human fibroblast cells (HFF) | Infection status:HCMV Sample source:Primary human fibroblast cells (HFF) | Infection status:HCMV Sample source:Primary human fibroblast cells (HFF) | Infection status:Uninfected Sample source:Primary human fibroblast cells (HFF) | Infection status:Uninfected Sample source:Primary human fibroblast cells (HFF) | Infection status:HCMV Sample source:Primary human fibroblast cells (HFF) | Infection status:HCMV Sample source:Primary human fibroblast cells (HFF) | Infection status:Uninfected Sample source:Primary human fibroblast cells (HFF) | Infection status:Uninfected Sample source:Primary human fibroblast cells (HFF) | Infection status:HCMV Sample source:Primary human fibroblast cells (HFF) | Infection status:HCMV Sample source:Primary human fibroblast cells (HFF) | Infection status:Uninfected Sample source:Primary human fibroblast cells (HFF) | Infection status:Uninfected Sample source:Primary human fibroblast cells (HFF) | Infection status:HCMV Sample source:Primary human fibroblast cells (HFF) | Infection status:HCMV Sample source:Primary human fibroblast cells (HFF) | Infection status:Uninfected Sample source:Primary human fibroblast cells (HFF) | Infection status:Uninfected Sample source:Primary human fibroblast cells (HFF) | Infection status:HCMV Sample source:Primary human fibroblast cells (HFF) | Infection status:HCMV PC 28:0 35955484 41815184 159416816 159066608 85874376 81972296 304382048 292764864 28822122 29794090 219635328 219747712 20146346 24236914 63061184 48821328 45284000 53468540 32990758 33177254 116951176 147483424 31095194 31811920 245198400 154646544 54393012 47059088 223868592 230298736 12964104 11817860 272342400 291922848 16572143 16323844 120667496 137205952 22098696 6021329.5 130260120 32804384 25418762 32305834 140207376 230671984 17410058 16989934 260735920 283577280 34966184 27471366 138410352 160757664 21996710 25341800 61728440 58460110 3897861 4219333 27601214 26569186 9196729 9133067 33594012 32961578 PC 28:1 1791470 1782519 5661407 5052867 17289478 14602887 50190764 50857600 4189352 4965945 19215486 21025874 203137 193415 717124 740145 624449 712526 2698054 2465403 7076386 10224333 1500375 1581972 15350372 9757857 2145783 1979151 40899476 32627126 393661 441013 5194812 5586934 816781 627211 2948367 3699594 747386.56 194894.52 3281408.25 820817.81 911508 1466135 4118859 7213817 449078 514779 5676300 6438770 1175973 1031902 4163714 4814911 755386 711919 2014695 1907993 230631 234404 799019 725731 646062 687303 1159835 1289343 PC 30:0 401060192 453309856 696460672 635894080 491934336 464821728 710551360 785689024 297402240 287369984 574207040 587095424 369573792 341753728 604102592 689189056 551107136 510359776 233180272 250646160 392132192 500431136 267835712 267690176 728580032 598599424 461311968 346777088 807273472 829534144 340828832 325427392 922937344 927244480 381013248 414135712 513751200 563592512 430109344 131286360 410174752 119282304 493447168 544595200 431422560 621270784 338607648 366107360 709204288 726315712 552383808 549891648 444849408 507538976 311120900 334316800 481985900 381050900 68488936 80496296 240114992 232361136 125609480 145219296 291423936 356110496 PC 32:0 717584064 786868736 514565472 514553952 670349888 623690176 427894560 425265824 508824576 473597280 300976512 331562240 693572160 738484736 694056256 637508096 502349920 403997728 274650368 317977440 264502144 300680160 435226240 421291872 494798368 407985280 711717952 622726208 697198656 636618176 810218816 803061312 916024000 925854016 983981568 1023878464 473587808 525151648 799463616 267557840 307043104 81998024 886572032 1056249664 317930400 477719392 642930560 650480320 531500064 514309376 1046383424 1069720064 328771360 379094016 523309000 511552300 498714900 337090200 171348192 244913232 285304064 289768224 301001184 323163168 333251648 381056160 PC 32:1 1382513280 1393524864 1971924480 1845670272 2416142336 2490595328 2671684864 2769019648 1831875200 1808202880 2028856704 2066470016 900048448 799238656 1838135168 1682798208 1519647232 1606455168 719767232 802686784 1460991360 1645864960 1010434112 1080442240 2755278848 2103131776 1556626560 1402062208 3314575360 2919602176 905094464 913469120 2683508224 2860929536 1047017472 1149880448 1609107072 1793560576 1171735040 404959328 1665963904 500401696 1279271296 1458128256 1814064768 2424811776 915125568 964958912 2479858688 2628647680 1353199232 1376834176 1622448512 1707837312 1036859000 1233676000 1695375000 1608147000 305789856 386194208 851678400 843775552 596834240 629220544 1196471424 1212854272 PC 32:2 179965200 183875840 212983600 187067024 782566592 730017728 738909504 763203392 401972512 397007360 417406624 418181376 64967328 69461384 152728912 156372272 106335912 116050392 131903544 152484144 238071600 274858208 171739536 154096000 405111712 300762848 251911984 229168144 529006336 498032128 69288232 77011784 259770224 291620128 85547336 97310328 113444616 136719008 100211560 35972584 123633216 36450296 114273080 140430544 143401264 250606768 77141624 89291104 251392336 266333696 138094384 145812336 119571040 148813008 105721200 100300900 154449400 137940000 23847498 31049426 70757992 59582384 46360736 48489188 120490968 125849688 PC 34:0 119717568 131855968 59421140 61064244 140954784 139629040 72167832 74458984 73851608 74674568 43313144 46278124 124602216 138769088 171135424 141393216 128973608 134387600 68177000 69780584 63586460 79945408 116997728 115742784 84686944 64852916 220722320 189685552 126872056 101669920 153883904 151775312 137772288 133555400 179361360 208841648 66112576 73254200 137408880 45384884 32165592 8339034 155312784 185923024 32133222 47871644 91924480 119074432 59653508 58081164 187947344 190600320 34163668 39582028 46858680 52707600 41179680 41179680 23193138 30858118 26258750 17839410 41028028 29895146 45517888 39536208 PC 34:1 1936433024 1787084160 2466488320 2717298688 2478959872 2525678848 2662124288 2968771328 2362292736 2297463040 2585455360 2590034944 1906209408 1754377216 4032020224 3880824832 3189473536 3108535296 998424128 1030540864 1490824832 1683553920 1499702400 1635400320 2531414272 2023668096 1910073984 1754889088 3320602880 3185028352 2894772992 2853198848 5351859712 5670318080 3175753728 3313478400 3926492416 4249953280 3141428992 1160731136 3584316160 1186507904 3603473664 3695642880 3880829696 4966727168 2577956608 2799986688 5035897856 4874519040 3641170688 3664720640 3782504192 4001532672 1731044000 1715775000 2323905000 2139411000 723902272 795946752 1489640576 1639628416 1021523648 1033309440 1732650624 2008369536 PC 34:2 825456192 811497408 872269632 779563712 1378481920 1365716096 1567923200 1610862976 916317504 857267904 1008598272 966071232 431518592 464953472 871532032 658897280 733015744 541652992 438769632 514979840 635649152 781797056 657204160 695439424 960000448 856927936 672741440 655455552 1204080256 1167299584 1115460096 1107264640 2280708352 2518642944 1186596736 1313071616 1254331392 1438535040 1452184576 714717568 1218622976 530413056 1439527552 1506449024 1303162752 1881760256 993161472 1161332096 1927770112 1964159488 1467222912 1557530240 1110348672 1238710784 650451500 716859700 649146800 646089000 167047136 235246160 350122432 353913472 321584736 321436224 480432800 548820416 PC 34:3 106526680 115697320 81907520 83166712 278471488 299040352 239039872 262401552 250511312 251914000 184320592 176612656 46632320 48663780 83337704 76508152 61955616 68055784 48698108 50768416 59728020 80886072 83441720 84276224 99321256 84542856 69901072 65260460 148069696 135573232 86478520 79669752 173814032 168788080 101269512 111221304 95037720 108212064 90112936 28396486 67845728 19531650 94597976 108555136 77188416 106231320 66801300 70041160 108471872 118307352 107893408 115164864 80806440 89593560 61715310 67301090 60294420 38989050 24644862 39376916 42097280 43440908 55095504 60404620 74433944 76580168 PC 34:4 22303386 23093840 21508410 21806302 76139680 74359400 52520128 54794400 48131316 49208716 37588924 36395308 14314664 15387667 25462152 26776186 17611942 16938828 5270442 6411406 10289211 12483131 9225951 10571832 12789968 9617715 15806385 13321221 26806914 23090766 7668098 7764808 39822692 40761332 9311484 10607351 17575640 20399226 8331270.5 3151971.25 15089512 3802815.75 10052224 11713717 17362198 29418582 6735448 7360168 26512738 26807490 13870553 13262944 18088422 21716610 9376321 9908476 12008180 11664860 3700929 4757050 11145884 10874725 6355590 4783142 15684595 18844114 PC 36:1 741318848 767103808 985307712 896331840 801449472 780067520 1091680768 1148295552 579073280 670802432 921774784 808515072 326205440 404898848 1021722368 1049918272 1177935232 1338993152 225931696 287369504 584025920 690843200 334451424 356062400 841884928 591936192 584139264 526683552 1154548864 1081065856 1092885632 1147792768 2365116416 2428500736 1250993664 1357101440 1391812736 1463090816 1462136448 508625312 1125148032 309926688 1460524416 1714896512 1129945984 1570251264 931425792 1096436096 1642290816 1811487104 1420379904 1565582720 1036481792 1101521536 350813900 396303000 566838700 435310100 106687808 131846944 342401504 284896448 247535504 237816624 570664768 645416576 PC 36:2 1058589184 1123595264 1165292544 1163617408 1174509568 1153768576 1481528448 1490087936 835737408 906837056 1102842880 1116016512 527405600 720998080 932241664 1114339968 1152338944 1027267904 605349952 672319168 794136384 910162112 985038784 1013068992 1199779200 964603840 970695424 939760960 1566612480 1521936896 1620724608 1629228672 2988470016 3085282560 1823696256 2011353728 1995498496 2138525696 2006460416 683307840 1695606272 577734464 2082035072 2248956928 1893931008 2477578240 1564723200 1711534720 2523256832 2718766080 2157798912 2148697856 1635181440 1803602560 835913800 910281200 967650500 858519700 294774688 354473184 557586752 552278848 477610016 551536320 810467648 845807808 PC 36:3 253824752 262552880 252984576 239143168 394709664 375572992 399872736 374260576 272134624 260355568 311650976 284048128 139624896 175734448 316275616 313524256 317276992 266319232 132422592 153706256 162580416 205640400 236977424 243135120 281850912 197465584 187050192 201380016 363702432 333622880 215091792 229396880 425260128 454369952 241018304 269276096 239850112 274142080 269565632 99769600 216448944 60140144 282609152 338094816 228213888 327202720 196336752 227989712 324867936 350599296 312785024 315560480 188582224 219797632 188208700 182540900 192273800 177497500 57403540 75778368 116830936 106218880 98489440 38133284 163156048 167141712 PC 36:4 206462384 209260224 201338640 192436224 273202784 277187968 179060944 171591536 331681888 301994656 233672832 222768192 151025520 189131472 440872704 344571872 283008096 207537648 40636492 45117844 74512200 94629920 67810024 66842388 93365128 87195352 116908768 119505152 218036480 214186768 409591968 413506656 832255296 829968192 467325440 515323104 447189152 464364928 347672288 158016320 338708448 135771888 379143168 388537888 362715392 460753632 242638080 293793536 453452032 452729056 407585920 415384064 392064256 418017952 96091660 104141400 138257300 132253300 53221820 80585944 150536336 146686192 82395192 78348984 164523072 196661520 PC 36:5 74663648 81285304 59694868 59145040 142289344 141951408 91871656 90393016 145081296 156509680 88895712 93717888 46526964 59424992 159997664 129169768 79471960 72262144 9130949 10587713 13204195 16942750 13216936 13447325 11703789 12123684 33390930 27703434 37764884 33848028 62193644 64269492 131334432 121575520 80622608 82801112 70911144 72042184 51491424 18943426 44439636 10769104 60972420 70064040 49945344 69129080 35865044 43516200 63468460 61174500 61991296 67304632 56019316 60741084 28821390 30594850 36081160 37253970 15679197 23714072 39510720 35174640 24500984 25643866 51119328 54503616 PC 38:1 16365665 19163512 27944346 26066544 10762539 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