#METABOLOMICS WORKBENCH Sethjparker_20250619_134606 DATATRACK_ID:6064 STUDY_ID:ST004018 ANALYSIS_ID:AN006624 PROJECT_ID:PR002501
VERSION             	1
CREATED_ON             	July 2, 2025, 11:16 am
#PROJECT
PR:PROJECT_TITLE                 	Metabolomics analysis of SNAT2-deficient cells: implications for the discovery
PR:PROJECT_TITLE                 	of selective transporter inhibitors
PR:PROJECT_TYPE                  	Manuscript
PR:PROJECT_SUMMARY               	Amino acid uptake by the solute carrier family of transporter proteins is
PR:PROJECT_SUMMARY               	critical to support cell metabolism, and inhibition of transporter activity
PR:PROJECT_SUMMARY               	represents a tractable strategy to restrict nutrient availability to cancer
PR:PROJECT_SUMMARY               	cells. A small molecule inhibitor of the sodium-coupled neutral amino acid
PR:PROJECT_SUMMARY               	transporter 2 (SNAT2),
PR:PROJECT_SUMMARY               	3-(N-methyl(4-methylphenyl)sulfonamido)-N-(2-trifluoromethylbenzyl)thiophene-2-carboxamide
PR:PROJECT_SUMMARY               	(MMTC/57E), was recently identified and was shown to inhibit cell proliferation
PR:PROJECT_SUMMARY               	when combined with glucose transport inhibitors in breast and pancreatic cancer
PR:PROJECT_SUMMARY               	cell lines. In this study, we use mass spectrometry-based metabolomics and
PR:PROJECT_SUMMARY               	establish cell-based assays for the SNAT2 transporter. We show that SNAT2
PR:PROJECT_SUMMARY               	knockout cells have significant defects in amino acid availability. Using our
PR:PROJECT_SUMMARY               	established assays, we fail to observe that MMTC/57E inhibits SNAT2 activity
PR:PROJECT_SUMMARY               	likely due to its poor solubility.
PR:INSTITUTE                     	University of British Columbia
PR:DEPARTMENT                    	Biochemistry & Molecular Biology
PR:LABORATORY                    	Parker laboratory
PR:LAST_NAME                     	Parker
PR:FIRST_NAME                    	Seth
PR:ADDRESS                       	950 W 28th Ave, Vancouver, British Columbia, V6H 0B3, Canada
PR:EMAIL                         	seth.parker@bcchr.ca
PR:PHONE                         	6048753121
#STUDY
ST:STUDY_TITLE                   	Evaluation of SNAT1-dependent MeAIB uptake using GCMS
ST:STUDY_SUMMARY                 	HY15549 cell lines were engineered to stably express a human SNAT1 cDNA or an
ST:STUDY_SUMMARY                 	empty vector (EV, controls) by lentiviral transduction. Either EV or +SNAT1 cell
ST:STUDY_SUMMARY                 	lines were plated and evaluated for MeAIB uptake by culturing cells with a
ST:STUDY_SUMMARY                 	gradient from 0.125-4 mM for 30-minutes in PBS for 30-minutes prior to GCMS
ST:STUDY_SUMMARY                 	analysis. SNAT1 exhibited saturable uptake kinetics with an apparent Km of ~2.91
ST:STUDY_SUMMARY                 	mM.
ST:INSTITUTE                     	University of British Columbia
ST:DEPARTMENT                    	Biochemistry & Molecular Biology
ST:LABORATORY                    	Parker laboratory
ST:LAST_NAME                     	Parker
ST:FIRST_NAME                    	Seth
ST:ADDRESS                       	950 W 28th Ave, Vancouver, British Columbia, V6H 0B3, Canada
ST:EMAIL                         	seth.parker@bcchr.ca
ST:PHONE                         	6048753121
#SUBJECT
SU:SUBJECT_TYPE                  	Mammal
SU:SUBJECT_SPECIES               	Mus musculus
SU:TAXONOMY_ID                   	10090
SU:GENDER                        	Female
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data
SUBJECT_SAMPLE_FACTORS           	1	HYiSN2_EV_dox_1a	Factor:EV | Doxycycline (y/n):y | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):0.15625	Replicate=1; Cell number (x1e6)=1.18675; RAW_FILE_NAME(Raw file name)=HYiSN2_EV_dox_1a.CDF
SUBJECT_SAMPLE_FACTORS           	2	HYiSN2_EV_dox_1b	Factor:EV | Doxycycline (y/n):y | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):0.15625	Replicate=2; Cell number (x1e6)=0.67175; RAW_FILE_NAME(Raw file name)=HYiSN2_EV_dox_1b.CDF
SUBJECT_SAMPLE_FACTORS           	3	HYiSN2_EV_dox_2a	Factor:EV | Doxycycline (y/n):y | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):0.3125	Replicate=1; Cell number (x1e6)=1.18675; RAW_FILE_NAME(Raw file name)=HYiSN2_EV_dox_2a.CDF
SUBJECT_SAMPLE_FACTORS           	4	HYiSN2_EV_dox_2b	Factor:EV | Doxycycline (y/n):y | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):0.3125	Replicate=2; Cell number (x1e6)=0.67175; RAW_FILE_NAME(Raw file name)=HYiSN2_EV_dox_2b.CDF
SUBJECT_SAMPLE_FACTORS           	5	HYiSN2_EV_dox_2c	Factor:EV | Doxycycline (y/n):y | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):0.3125	Replicate=3; Cell number (x1e6)=0.69675; RAW_FILE_NAME(Raw file name)=HYiSN2_EV_dox_2c.CDF
SUBJECT_SAMPLE_FACTORS           	6	HYiSN2_EV_dox_3a	Factor:EV | Doxycycline (y/n):y | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):0.625	Replicate=1; Cell number (x1e6)=1.18675; RAW_FILE_NAME(Raw file name)=HYiSN2_EV_dox_3a.CDF
SUBJECT_SAMPLE_FACTORS           	7	HYiSN2_EV_dox_3b	Factor:EV | Doxycycline (y/n):y | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):0.625	Replicate=2; Cell number (x1e6)=0.67175; RAW_FILE_NAME(Raw file name)=HYiSN2_EV_dox_3b.CDF
SUBJECT_SAMPLE_FACTORS           	8	HYiSN2_EV_dox_3c	Factor:EV | Doxycycline (y/n):y | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):0.625	Replicate=3; Cell number (x1e6)=0.69675; RAW_FILE_NAME(Raw file name)=HYiSN2_EV_dox_3c.CDF
SUBJECT_SAMPLE_FACTORS           	9	HYiSN2_EV_dox_4a	Factor:EV | Doxycycline (y/n):y | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):1.25	Replicate=1; Cell number (x1e6)=1.18675; RAW_FILE_NAME(Raw file name)=HYiSN2_EV_dox_4a.CDF
SUBJECT_SAMPLE_FACTORS           	10	HYiSN2_EV_dox_4b	Factor:EV | Doxycycline (y/n):y | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):1.25	Replicate=2; Cell number (x1e6)=0.67175; RAW_FILE_NAME(Raw file name)=HYiSN2_EV_dox_4b.CDF
SUBJECT_SAMPLE_FACTORS           	11	HYiSN2_EV_dox_4c	Factor:EV | Doxycycline (y/n):y | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):1.25	Replicate=3; Cell number (x1e6)=0.69675; RAW_FILE_NAME(Raw file name)=HYiSN2_EV_dox_4c.CDF
SUBJECT_SAMPLE_FACTORS           	12	HYiSN2_EV_dox_5a	Factor:EV | Doxycycline (y/n):y | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):2.5	Replicate=1; Cell number (x1e6)=1.18675; RAW_FILE_NAME(Raw file name)=HYiSN2_EV_dox_5a.CDF
SUBJECT_SAMPLE_FACTORS           	13	HYiSN2_EV_dox_5b	Factor:EV | Doxycycline (y/n):y | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):2.5	Replicate=2; Cell number (x1e6)=0.67175; RAW_FILE_NAME(Raw file name)=HYiSN2_EV_dox_5b.CDF
SUBJECT_SAMPLE_FACTORS           	14	HYiSN2_EV_dox_5c	Factor:EV | Doxycycline (y/n):y | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):2.5	Replicate=3; Cell number (x1e6)=0.69675; RAW_FILE_NAME(Raw file name)=HYiSN2_EV_dox_5c.CDF
SUBJECT_SAMPLE_FACTORS           	15	HYiSN2_EV_dox_6a	Factor:EV | Doxycycline (y/n):y | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):5	Replicate=1; Cell number (x1e6)=1.18675; RAW_FILE_NAME(Raw file name)=HYiSN2_EV_dox_6a.CDF
SUBJECT_SAMPLE_FACTORS           	16	HYiSN2_EV_dox_6b	Factor:EV | Doxycycline (y/n):y | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):5	Replicate=2; Cell number (x1e6)=0.67175; RAW_FILE_NAME(Raw file name)=HYiSN2_EV_dox_6b.CDF
SUBJECT_SAMPLE_FACTORS           	17	HYiSN2_EV_dox_6c	Factor:EV | Doxycycline (y/n):y | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):5	Replicate=3; Cell number (x1e6)=0.69675; RAW_FILE_NAME(Raw file name)=HYiSN2_EV_dox_6c.CDF
SUBJECT_SAMPLE_FACTORS           	18	HYiSN2_EV_nodox_1a	Factor:EV | Doxycycline (y/n):n | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):0.15625	Replicate=1; Cell number (x1e6)=1.1685; RAW_FILE_NAME(Raw file name)=HYiSN2_EV_nodox_1a.CDF
SUBJECT_SAMPLE_FACTORS           	19	HYiSN2_EV_nodox_1b	Factor:EV | Doxycycline (y/n):n | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):0.15625	Replicate=2; Cell number (x1e6)=0.55225; RAW_FILE_NAME(Raw file name)=HYiSN2_EV_nodox_1b.CDF
SUBJECT_SAMPLE_FACTORS           	20	HYiSN2_EV_nodox_1c	Factor:EV | Doxycycline (y/n):n | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):0.15625	Replicate=3; Cell number (x1e6)=0.6175; RAW_FILE_NAME(Raw file name)=HYiSN2_EV_nodox_1c.CDF
SUBJECT_SAMPLE_FACTORS           	21	HYiSN2_EV_nodox_2a	Factor:EV | Doxycycline (y/n):n | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):0.3125	Replicate=1; Cell number (x1e6)=1.1685; RAW_FILE_NAME(Raw file name)=HYiSN2_EV_nodox_2a.CDF
SUBJECT_SAMPLE_FACTORS           	22	HYiSN2_EV_nodox_2b	Factor:EV | Doxycycline (y/n):n | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):0.3125	Replicate=2; Cell number (x1e6)=0.55225; RAW_FILE_NAME(Raw file name)=HYiSN2_EV_nodox_2b.CDF
SUBJECT_SAMPLE_FACTORS           	23	HYiSN2_EV_nodox_2c	Factor:EV | Doxycycline (y/n):n | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):0.3125	Replicate=3; Cell number (x1e6)=0.6175; RAW_FILE_NAME(Raw file name)=HYiSN2_EV_nodox_2c.CDF
SUBJECT_SAMPLE_FACTORS           	24	HYiSN2_EV_nodox_3a	Factor:EV | Doxycycline (y/n):n | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):0.625	Replicate=1; Cell number (x1e6)=1.1685; RAW_FILE_NAME(Raw file name)=HYiSN2_EV_nodox_3a.CDF
SUBJECT_SAMPLE_FACTORS           	25	HYiSN2_EV_nodox_3b	Factor:EV | Doxycycline (y/n):n | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):0.625	Replicate=2; Cell number (x1e6)=0.55225; RAW_FILE_NAME(Raw file name)=HYiSN2_EV_nodox_3b.CDF
SUBJECT_SAMPLE_FACTORS           	26	HYiSN2_EV_nodox_3c	Factor:EV | Doxycycline (y/n):n | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):0.625	Replicate=3; Cell number (x1e6)=0.6175; RAW_FILE_NAME(Raw file name)=HYiSN2_EV_nodox_3c.CDF
SUBJECT_SAMPLE_FACTORS           	27	HYiSN2_EV_nodox_4a	Factor:EV | Doxycycline (y/n):n | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):1.25	Replicate=1; Cell number (x1e6)=1.1685; RAW_FILE_NAME(Raw file name)=HYiSN2_EV_nodox_4a.CDF
SUBJECT_SAMPLE_FACTORS           	28	HYiSN2_EV_nodox_4b	Factor:EV | Doxycycline (y/n):n | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):1.25	Replicate=2; Cell number (x1e6)=0.55225; RAW_FILE_NAME(Raw file name)=HYiSN2_EV_nodox_4b.CDF
SUBJECT_SAMPLE_FACTORS           	29	HYiSN2_EV_nodox_4c	Factor:EV | Doxycycline (y/n):n | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):1.25	Replicate=3; Cell number (x1e6)=0.6175; RAW_FILE_NAME(Raw file name)=HYiSN2_EV_nodox_4c.CDF
SUBJECT_SAMPLE_FACTORS           	30	HYiSN2_EV_nodox_5a	Factor:EV | Doxycycline (y/n):n | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):2.5	Replicate=1; Cell number (x1e6)=1.1685; RAW_FILE_NAME(Raw file name)=HYiSN2_EV_nodox_5a.CDF
SUBJECT_SAMPLE_FACTORS           	31	HYiSN2_EV_nodox_5b	Factor:EV | Doxycycline (y/n):n | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):2.5	Replicate=2; Cell number (x1e6)=0.55225; RAW_FILE_NAME(Raw file name)=HYiSN2_EV_nodox_5b.CDF
SUBJECT_SAMPLE_FACTORS           	32	HYiSN2_EV_nodox_5c	Factor:EV | Doxycycline (y/n):n | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):2.5	Replicate=3; Cell number (x1e6)=0.6175; RAW_FILE_NAME(Raw file name)=HYiSN2_EV_nodox_5c.CDF
SUBJECT_SAMPLE_FACTORS           	33	HYiSN2_EV_nodox_6a	Factor:EV | Doxycycline (y/n):n | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):5	Replicate=1; Cell number (x1e6)=1.1685; RAW_FILE_NAME(Raw file name)=HYiSN2_EV_nodox_6a.CDF
SUBJECT_SAMPLE_FACTORS           	34	HYiSN2_EV_nodox_6b	Factor:EV | Doxycycline (y/n):n | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):5	Replicate=2; Cell number (x1e6)=0.55225; RAW_FILE_NAME(Raw file name)=HYiSN2_EV_nodox_6b.CDF
SUBJECT_SAMPLE_FACTORS           	35	HYiSN2_EV_nodox_6c	Factor:EV | Doxycycline (y/n):n | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):5	Replicate=3; Cell number (x1e6)=0.6175; RAW_FILE_NAME(Raw file name)=HYiSN2_EV_nodox_6c.CDF
SUBJECT_SAMPLE_FACTORS           	36	HYiSN2_SN1_dox_1a	Factor:SNAT1 | Doxycycline (y/n):y | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):0.15625	Replicate=1; Cell number (x1e6)=1.5236; RAW_FILE_NAME(Raw file name)=HYiSN2_SN1_dox_1a.CDF
SUBJECT_SAMPLE_FACTORS           	37	HYiSN2_SN1_dox_1b	Factor:SNAT1 | Doxycycline (y/n):y | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):0.15625	Replicate=2; Cell number (x1e6)=0.8685; RAW_FILE_NAME(Raw file name)=HYiSN2_SN1_dox_1b.CDF
SUBJECT_SAMPLE_FACTORS           	38	HYiSN2_SN1_dox_1c	Factor:SNAT1 | Doxycycline (y/n):y | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):0.15625	Replicate=3; Cell number (x1e6)=0.63125; RAW_FILE_NAME(Raw file name)=HYiSN2_SN1_dox_1c.CDF
SUBJECT_SAMPLE_FACTORS           	39	HYiSN2_SN1_dox_2a	Factor:SNAT1 | Doxycycline (y/n):y | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):0.3125	Replicate=1; Cell number (x1e6)=1.5236; RAW_FILE_NAME(Raw file name)=HYiSN2_SN1_dox_2a.CDF
SUBJECT_SAMPLE_FACTORS           	40	HYiSN2_SN1_dox_2b	Factor:SNAT1 | Doxycycline (y/n):y | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):0.3125	Replicate=2; Cell number (x1e6)=0.8685; RAW_FILE_NAME(Raw file name)=HYiSN2_SN1_dox_2b.CDF
SUBJECT_SAMPLE_FACTORS           	41	HYiSN2_SN1_dox_2c	Factor:SNAT1 | Doxycycline (y/n):y | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):0.3125	Replicate=3; Cell number (x1e6)=0.63125; RAW_FILE_NAME(Raw file name)=HYiSN2_SN1_dox_2c.CDF
SUBJECT_SAMPLE_FACTORS           	42	HYiSN2_SN1_dox_3a	Factor:SNAT1 | Doxycycline (y/n):y | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):0.625	Replicate=1; Cell number (x1e6)=1.5236; RAW_FILE_NAME(Raw file name)=HYiSN2_SN1_dox_3a.CDF
SUBJECT_SAMPLE_FACTORS           	43	HYiSN2_SN1_dox_3b	Factor:SNAT1 | Doxycycline (y/n):y | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):0.625	Replicate=2; Cell number (x1e6)=0.8685; RAW_FILE_NAME(Raw file name)=HYiSN2_SN1_dox_3b.CDF
SUBJECT_SAMPLE_FACTORS           	44	HYiSN2_SN1_dox_3c	Factor:SNAT1 | Doxycycline (y/n):y | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):0.625	Replicate=3; Cell number (x1e6)=0.63125; RAW_FILE_NAME(Raw file name)=HYiSN2_SN1_dox_3c.CDF
SUBJECT_SAMPLE_FACTORS           	45	HYiSN2_SN1_dox_4a	Factor:SNAT1 | Doxycycline (y/n):y | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):1.25	Replicate=1; Cell number (x1e6)=1.5236; RAW_FILE_NAME(Raw file name)=HYiSN2_SN1_dox_4a.CDF
SUBJECT_SAMPLE_FACTORS           	46	HYiSN2_SN1_dox_4b	Factor:SNAT1 | Doxycycline (y/n):y | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):1.25	Replicate=2; Cell number (x1e6)=0.8685; RAW_FILE_NAME(Raw file name)=HYiSN2_SN1_dox_4b.CDF
SUBJECT_SAMPLE_FACTORS           	47	HYiSN2_SN1_dox_4c	Factor:SNAT1 | Doxycycline (y/n):y | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):1.25	Replicate=3; Cell number (x1e6)=0.63125; RAW_FILE_NAME(Raw file name)=HYiSN2_SN1_dox_4c.CDF
SUBJECT_SAMPLE_FACTORS           	48	HYiSN2_SN1_dox_5a	Factor:SNAT1 | Doxycycline (y/n):y | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):2.5	Replicate=1; Cell number (x1e6)=1.5236; RAW_FILE_NAME(Raw file name)=HYiSN2_SN1_dox_5a.CDF
SUBJECT_SAMPLE_FACTORS           	49	HYiSN2_SN1_dox_5b	Factor:SNAT1 | Doxycycline (y/n):y | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):2.5	Replicate=2; Cell number (x1e6)=0.8685; RAW_FILE_NAME(Raw file name)=HYiSN2_SN1_dox_5b.CDF
SUBJECT_SAMPLE_FACTORS           	50	HYiSN2_SN1_dox_5c	Factor:SNAT1 | Doxycycline (y/n):y | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):2.5	Replicate=3; Cell number (x1e6)=0.63125; RAW_FILE_NAME(Raw file name)=HYiSN2_SN1_dox_5c.CDF
SUBJECT_SAMPLE_FACTORS           	51	HYiSN2_SN1_dox_6a	Factor:SNAT1 | Doxycycline (y/n):y | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):5	Replicate=1; Cell number (x1e6)=1.5236; RAW_FILE_NAME(Raw file name)=HYiSN2_SN1_dox_6a.CDF
SUBJECT_SAMPLE_FACTORS           	52	HYiSN2_SN1_dox_6b	Factor:SNAT1 | Doxycycline (y/n):y | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):5	Replicate=2; Cell number (x1e6)=0.8685; RAW_FILE_NAME(Raw file name)=HYiSN2_SN1_dox_6b.CDF
SUBJECT_SAMPLE_FACTORS           	53	HYiSN2_SN1_dox_6c	Factor:SNAT1 | Doxycycline (y/n):y | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):5	Replicate=3; Cell number (x1e6)=0.63125; RAW_FILE_NAME(Raw file name)=HYiSN2_SN1_dox_6c.CDF
SUBJECT_SAMPLE_FACTORS           	54	HYiSN2_SN1_nodox_1a	Factor:SNAT1 | Doxycycline (y/n):n | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):0.15625	Replicate=1; Cell number (x1e6)=2.007; RAW_FILE_NAME(Raw file name)=HYiSN2_SN1_nodox_1a.CDF
SUBJECT_SAMPLE_FACTORS           	55	HYiSN2_SN1_nodox_1b	Factor:SNAT1 | Doxycycline (y/n):n | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):0.15625	Replicate=2; Cell number (x1e6)=0.60675; RAW_FILE_NAME(Raw file name)=HYiSN2_SN1_nodox_1b.CDF
SUBJECT_SAMPLE_FACTORS           	56	HYiSN2_SN1_nodox_1c	Factor:SNAT1 | Doxycycline (y/n):n | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):0.15625	Replicate=3; Cell number (x1e6)=0.58775; RAW_FILE_NAME(Raw file name)=HYiSN2_SN1_nodox_1c.CDF
SUBJECT_SAMPLE_FACTORS           	57	HYiSN2_SN1_nodox_2a	Factor:SNAT1 | Doxycycline (y/n):n | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):0.3125	Replicate=1; Cell number (x1e6)=2.007; RAW_FILE_NAME(Raw file name)=HYiSN2_SN1_nodox_2a.CDF
SUBJECT_SAMPLE_FACTORS           	58	HYiSN2_SN1_nodox_2b	Factor:SNAT1 | Doxycycline (y/n):n | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):0.3125	Replicate=2; Cell number (x1e6)=0.60675; RAW_FILE_NAME(Raw file name)=HYiSN2_SN1_nodox_2b.CDF
SUBJECT_SAMPLE_FACTORS           	59	HYiSN2_SN1_nodox_2c	Factor:SNAT1 | Doxycycline (y/n):n | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):0.3125	Replicate=3; Cell number (x1e6)=0.58775; RAW_FILE_NAME(Raw file name)=HYiSN2_SN1_nodox_2c.CDF
SUBJECT_SAMPLE_FACTORS           	60	HYiSN2_SN1_nodox_3a	Factor:SNAT1 | Doxycycline (y/n):n | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):0.625	Replicate=1; Cell number (x1e6)=2.007; RAW_FILE_NAME(Raw file name)=HYiSN2_SN1_nodox_3a.CDF
SUBJECT_SAMPLE_FACTORS           	61	HYiSN2_SN1_nodox_3b	Factor:SNAT1 | Doxycycline (y/n):n | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):0.625	Replicate=2; Cell number (x1e6)=0.60675; RAW_FILE_NAME(Raw file name)=HYiSN2_SN1_nodox_3b.CDF
SUBJECT_SAMPLE_FACTORS           	62	HYiSN2_SN1_nodox_3c	Factor:SNAT1 | Doxycycline (y/n):n | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):0.625	Replicate=3; Cell number (x1e6)=0.58775; RAW_FILE_NAME(Raw file name)=HYiSN2_SN1_nodox_3c.CDF
SUBJECT_SAMPLE_FACTORS           	63	HYiSN2_SN1_nodox_4a	Factor:SNAT1 | Doxycycline (y/n):n | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):1.25	Replicate=1; Cell number (x1e6)=2.007; RAW_FILE_NAME(Raw file name)=HYiSN2_SN1_nodox_4a.CDF
SUBJECT_SAMPLE_FACTORS           	64	HYiSN2_SN1_nodox_4b	Factor:SNAT1 | Doxycycline (y/n):n | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):1.25	Replicate=2; Cell number (x1e6)=0.60675; RAW_FILE_NAME(Raw file name)=HYiSN2_SN1_nodox_4b.CDF
SUBJECT_SAMPLE_FACTORS           	65	HYiSN2_SN1_nodox_4c	Factor:SNAT1 | Doxycycline (y/n):n | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):1.25	Replicate=3; Cell number (x1e6)=0.58775; RAW_FILE_NAME(Raw file name)=HYiSN2_SN1_nodox_4c.CDF
SUBJECT_SAMPLE_FACTORS           	66	HYiSN2_SN1_nodox_5a	Factor:SNAT1 | Doxycycline (y/n):n | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):2.5	Replicate=1; Cell number (x1e6)=2.007; RAW_FILE_NAME(Raw file name)=HYiSN2_SN1_nodox_5a.CDF
SUBJECT_SAMPLE_FACTORS           	67	HYiSN2_SN1_nodox_5b	Factor:SNAT1 | Doxycycline (y/n):n | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):2.5	Replicate=2; Cell number (x1e6)=0.60675; RAW_FILE_NAME(Raw file name)=HYiSN2_SN1_nodox_5b.CDF
SUBJECT_SAMPLE_FACTORS           	68	HYiSN2_SN1_nodox_5c	Factor:SNAT1 | Doxycycline (y/n):n | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):2.5	Replicate=3; Cell number (x1e6)=0.58775; RAW_FILE_NAME(Raw file name)=HYiSN2_SN1_nodox_5c.CDF
SUBJECT_SAMPLE_FACTORS           	69	HYiSN2_SN1_nodox_6a	Factor:SNAT1 | Doxycycline (y/n):n | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):5	Replicate=1; Cell number (x1e6)=2.007; RAW_FILE_NAME(Raw file name)=HYiSN2_SN1_nodox_6a.CDF
SUBJECT_SAMPLE_FACTORS           	70	HYiSN2_SN1_nodox_6b	Factor:SNAT1 | Doxycycline (y/n):n | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):5	Replicate=2; Cell number (x1e6)=0.60675; RAW_FILE_NAME(Raw file name)=HYiSN2_SN1_nodox_6b.CDF
SUBJECT_SAMPLE_FACTORS           	71	HYiSN2_SN1_nodox_6c	Factor:SNAT1 | Doxycycline (y/n):n | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):5	Replicate=3; Cell number (x1e6)=0.58775; RAW_FILE_NAME(Raw file name)=HYiSN2_SN1_nodox_6c.CDF
#COLLECTION
CO:COLLECTION_SUMMARY            	Cells were washed twice with room temperature (20°C) PBS before adding 2 mL of
CO:COLLECTION_SUMMARY            	PBS containing 0.16-5 mM of MeAIB. After a 30-minute incubation at 20°C, the
CO:COLLECTION_SUMMARY            	transport buffer was aspirated and the cells were washed three times with ice
CO:COLLECTION_SUMMARY            	cold 0.9% NaCl. To extract metabolites, 1 mL of ice cold 80% methanol containing
CO:COLLECTION_SUMMARY            	2.5 nmol of 13C3-alanine was added to each well. The plates were shaken at ~500
CO:COLLECTION_SUMMARY            	rpm for 15 minutes at room temperature and 900 µL was transferred to a new tube
CO:COLLECTION_SUMMARY            	and dried using a SpeedVac for GCMS analysis.
CO:SAMPLE_TYPE                   	Tumor cells
#TREATMENT
TR:TREATMENT_SUMMARY             	No treatment.
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	Dried samples were derivatized for GCMS analysis. 25 µL of methoxyamine
SP:SAMPLEPREP_SUMMARY            	hydrochloride (20 mg/mL, prepared in pyridine) was added to each tube, which was
SP:SAMPLEPREP_SUMMARY            	vortexed and incubated at 37°C for 60 minutes. Following the incubation, 25 µL
SP:SAMPLEPREP_SUMMARY            	of MTBSTFA + 1% TBDMSCl was added to each tube, which was vortexed and
SP:SAMPLEPREP_SUMMARY            	centrifuged at 21,300 x g for 15-minutes at 20°C. 40 µL of the resulting
SP:SAMPLEPREP_SUMMARY            	supernatant was transferred to a plastic sample vial for GCMS analysis.
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_SUMMARY        	Agilent DB35-MS (30m x 0.25mm, 0.25µm) was interfaced with an inert column (5m x
CH:CHROMATOGRAPHY_SUMMARY        	0.15mm, 0 µm) to the MSD. Initial oven temperature maintained at 75°C for 3
CH:CHROMATOGRAPHY_SUMMARY        	minutes, ramped to 255°C at 7.5°C/min, held for 10 minutes, ramped to 320°C at 
CH:CHROMATOGRAPHY_SUMMARY        	10°C/min, held for 5 minutes. The inlet was maintained at 270°C in splitless mode;
CH:CHROMATOGRAPHY_SUMMARY        	an injection volume of 1 µL was used for all samples.
CH:CHROMATOGRAPHY_TYPE           	GC
CH:INSTRUMENT_NAME               	Agilent 8890
CH:COLUMN_NAME                   	Agilent DB-35MS (30m x 0.25mm, 0.25µm) interfaced with an inert column (5m x 0.15mm, 0 µm)
CH:SOLVENT_A                     	UHP Helium
CH:SOLVENT_B                     	None
CH:FLOW_GRADIENT                 	100% Helium
CH:FLOW_RATE                     	1 mL/min
CH:COLUMN_TEMPERATURE            	75°C
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
#MS
MS:INSTRUMENT_NAME               	Agilent 5977B
MS:INSTRUMENT_TYPE               	Single quadrupole
MS:MS_TYPE                       	EI
MS:ION_MODE                      	POSITIVE
MS:MS_COMMENTS                   	The MS was operated in full-scan mode from 100 to 650 m/z with a threshold of
MS:MS_COMMENTS                   	150, a scan speed of 781 u/sec, a frequency of 1.4 scans/sec, a cycle time of
MS:MS_COMMENTS                   	723 ms, and a step size of 0.1 m/z. The MS Source and MS Quad temperatures were
MS:MS_COMMENTS                   	set to 230°C and 150°C, respectively. A gain factor of 1 was used.
#MS_METABOLITE_DATA
MS_METABOLITE_DATA:UNITS	ion counts
MS_METABOLITE_DATA_START
Samples	HYiSN2_EV_dox_1b	HYiSN2_EV_dox_2b	HYiSN2_EV_dox_3b	HYiSN2_EV_dox_4b	HYiSN2_EV_dox_5b	HYiSN2_EV_dox_6b	HYiSN2_EV_nodox_1b	HYiSN2_EV_nodox_2b	HYiSN2_EV_nodox_3b	HYiSN2_EV_nodox_4b	HYiSN2_EV_nodox_5b	HYiSN2_EV_nodox_6b	HYiSN2_SN1_dox_1b	HYiSN2_SN1_dox_2b	HYiSN2_SN1_dox_3b	HYiSN2_SN1_dox_4b	HYiSN2_SN1_dox_5b	HYiSN2_SN1_dox_6b	HYiSN2_SN1_nodox_1b	HYiSN2_SN1_nodox_2b	HYiSN2_SN1_nodox_3b	HYiSN2_SN1_nodox_4b	HYiSN2_SN1_nodox_5b	HYiSN2_SN1_nodox_6b	HYiSN2_EV_dox_1a	HYiSN2_EV_dox_2a	HYiSN2_EV_dox_3a	HYiSN2_EV_dox_4a	HYiSN2_EV_dox_5a	HYiSN2_EV_dox_6a	HYiSN2_EV_nodox_1a	HYiSN2_EV_nodox_2a	HYiSN2_EV_nodox_3a	HYiSN2_EV_nodox_4a	HYiSN2_EV_nodox_5a	HYiSN2_EV_nodox_6a	HYiSN2_SN1_dox_1a	HYiSN2_SN1_dox_2a	HYiSN2_SN1_dox_3a	HYiSN2_SN1_dox_4a	HYiSN2_SN1_dox_5a	HYiSN2_SN1_dox_6a	HYiSN2_SN1_nodox_1a	HYiSN2_SN1_nodox_2a	HYiSN2_SN1_nodox_3a	HYiSN2_SN1_nodox_4a	HYiSN2_SN1_nodox_5a	HYiSN2_SN1_nodox_6a	HYiSN2_EV_dox_2c	HYiSN2_EV_dox_3c	HYiSN2_EV_dox_4c	HYiSN2_EV_dox_5c	HYiSN2_EV_dox_6c	HYiSN2_EV_nodox_1c	HYiSN2_EV_nodox_2c	HYiSN2_EV_nodox_3c	HYiSN2_EV_nodox_4c	HYiSN2_EV_nodox_5c	HYiSN2_EV_nodox_6c	HYiSN2_SN1_dox_1c	HYiSN2_SN1_dox_2c	HYiSN2_SN1_dox_3c	HYiSN2_SN1_dox_4c	HYiSN2_SN1_dox_5c	HYiSN2_SN1_dox_6c	HYiSN2_SN1_nodox_1c	HYiSN2_SN1_nodox_2c	HYiSN2_SN1_nodox_3c	HYiSN2_SN1_nodox_4c	HYiSN2_SN1_nodox_5c	HYiSN2_SN1_nodox_6c
Factors	Factor:EV | Doxycycline (y/n):y | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):0.15625	Factor:EV | Doxycycline (y/n):y | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):0.3125	Factor:EV | Doxycycline (y/n):y | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):0.625	Factor:EV | Doxycycline (y/n):y | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):1.25	Factor:EV | Doxycycline (y/n):y | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):2.5	Factor:EV | Doxycycline (y/n):y | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):5	Factor:EV | Doxycycline (y/n):n | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):0.15625	Factor:EV | Doxycycline (y/n):n | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):0.3125	Factor:EV | Doxycycline (y/n):n | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):0.625	Factor:EV | Doxycycline (y/n):n | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):1.25	Factor:EV | Doxycycline (y/n):n | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):2.5	Factor:EV | Doxycycline (y/n):n | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):5	Factor:SNAT1 | Doxycycline (y/n):y | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):0.15625	Factor:SNAT1 | Doxycycline (y/n):y | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):0.3125	Factor:SNAT1 | Doxycycline (y/n):y | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):0.625	Factor:SNAT1 | Doxycycline (y/n):y | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):1.25	Factor:SNAT1 | Doxycycline (y/n):y | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):2.5	Factor:SNAT1 | Doxycycline (y/n):y | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):5	Factor:SNAT1 | Doxycycline (y/n):n | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):0.15625	Factor:SNAT1 | Doxycycline (y/n):n | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):0.3125	Factor:SNAT1 | Doxycycline (y/n):n | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):0.625	Factor:SNAT1 | Doxycycline (y/n):n | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):1.25	Factor:SNAT1 | Doxycycline (y/n):n | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):2.5	Factor:SNAT1 | Doxycycline (y/n):n | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):5	Factor:EV | Doxycycline (y/n):y | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):0.15625	Factor:EV | Doxycycline (y/n):y | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):0.3125	Factor:EV | Doxycycline (y/n):y | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):0.625	Factor:EV | Doxycycline (y/n):y | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):1.25	Factor:EV | Doxycycline (y/n):y | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):2.5	Factor:EV | Doxycycline (y/n):y | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):5	Factor:EV | Doxycycline (y/n):n | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):0.15625	Factor:EV | Doxycycline (y/n):n | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):0.3125	Factor:EV | Doxycycline (y/n):n | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):0.625	Factor:EV | Doxycycline (y/n):n | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):1.25	Factor:EV | Doxycycline (y/n):n | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):2.5	Factor:EV | Doxycycline (y/n):n | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):5	Factor:SNAT1 | Doxycycline (y/n):y | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):0.15625	Factor:SNAT1 | Doxycycline (y/n):y | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):0.3125	Factor:SNAT1 | Doxycycline (y/n):y | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):0.625	Factor:SNAT1 | Doxycycline (y/n):y | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):1.25	Factor:SNAT1 | Doxycycline (y/n):y | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):2.5	Factor:SNAT1 | Doxycycline (y/n):y | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):5	Factor:SNAT1 | Doxycycline (y/n):n | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):0.15625	Factor:SNAT1 | Doxycycline (y/n):n | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):0.3125	Factor:SNAT1 | Doxycycline (y/n):n | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):0.625	Factor:SNAT1 | Doxycycline (y/n):n | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):1.25	Factor:SNAT1 | Doxycycline (y/n):n | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):2.5	Factor:SNAT1 | Doxycycline (y/n):n | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):5	Factor:EV | Doxycycline (y/n):y | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):0.3125	Factor:EV | Doxycycline (y/n):y | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):0.625	Factor:EV | Doxycycline (y/n):y | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):1.25	Factor:EV | Doxycycline (y/n):y | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):2.5	Factor:EV | Doxycycline (y/n):y | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):5	Factor:EV | Doxycycline (y/n):n | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):0.15625	Factor:EV | Doxycycline (y/n):n | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):0.3125	Factor:EV | Doxycycline (y/n):n | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):0.625	Factor:EV | Doxycycline (y/n):n | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):1.25	Factor:EV | Doxycycline (y/n):n | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):2.5	Factor:EV | Doxycycline (y/n):n | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):5	Factor:SNAT1 | Doxycycline (y/n):y | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):0.15625	Factor:SNAT1 | Doxycycline (y/n):y | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):0.3125	Factor:SNAT1 | Doxycycline (y/n):y | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):0.625	Factor:SNAT1 | Doxycycline (y/n):y | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):1.25	Factor:SNAT1 | Doxycycline (y/n):y | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):2.5	Factor:SNAT1 | Doxycycline (y/n):y | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):5	Factor:SNAT1 | Doxycycline (y/n):n | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):0.15625	Factor:SNAT1 | Doxycycline (y/n):n | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):0.3125	Factor:SNAT1 | Doxycycline (y/n):n | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):0.625	Factor:SNAT1 | Doxycycline (y/n):n | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):1.25	Factor:SNAT1 | Doxycycline (y/n):n | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):2.5	Factor:SNAT1 | Doxycycline (y/n):n | Sample source:HY15549 Pancreatic Cancer Cells | MeAIB concentration (mM):5
MeAIB_174	3185117	5256097	8771511	11103519	12829914	14662661	297479	460208	723012	925090	1261669	1979006	4283285	8788737	17468131	29153888	42755761	45853025	3414202	8016326	14038877	23540039	37438733	40417145	3253177	5673405	8102327	14235524	17288038	16987933	258676	324614	478319	566645	1030666	1462406	4722742	8606907	18833341	30312553	42694242	53027033	2351048	5414611	10854016	18988888	34282795	41130952	6182478	9413412	11019237	14387271	14954320	289373	467421	635203	649765	1146937	2038290	5229688	10752242	18925548	26778518	37235598	38908481	3242759	8637096	15472333	22188416	29563609	39428768
Ala_232	17196294	17145251	13924233	18446409	19151208	19314771	10943546	9599352	11842356	8497097	7839577	7815166	10537188	8535516	11166446	11942936	13799729	13049182	12248962	11490710	12935050	9565748	10211091	8441061	11596194	13830363	18602324	31591086	37902733	41337807	7960541	8559875	7574765	15447267	8833965	4840929	10725887	8233569	10277664	14371165	14725364	13981147	5275213	5693553	5667025	5143969	6101823	9140229	9590216	11815935	16051493	17724582	20908823	4393544	4441793	7450477	5988626	7104765	8548531	8368582	10157384	11927714	11047597	12017152	9014709	6383582	7474132	8404625	8491802	5015949	5184288
Ala_260	5754421	6793573	10543318	12738685	15652604	14823129	3020134	2899340	3350361	3340210	2831065	2956305	4691419	6119088	9085307	9586058	11298769	10087260	4509189	4575220	4014884	4193304	4881461	3730900	9015519	11151124	15016551	27136050	33152698	34079632	2957936	2820443	2884213	3091522	2904695	2149260	7341566	5402365	7213055	10919554	10173850	10254999	3388597	3521455	3547852	3260683	4114847	3324722	7713959	10287275	14334810	15568683	16571675	3025364	3243208	2466127	2249055	2843346	2949963	5822781	6314915	9026549	8030899	8907757	6732225	3353382	3895502	4033175	3879219	2340405	2884690
Ala_264	2649335.428	2536040.801	2896249.455	2696779.615	2964603.198	2617764.581	2418825.321	2264094.606	2538568.53	2485116.24	2139718.927	2206586.052	2288474.188	2087832.826	2521172.693	2687930.663	3108291.352	2551068.054	2599096.54	2475194.02	2302535.974	2318477.782	2854678.393	2041921.57	2111434.55	2089720.638	1835022.532	2396113.215	2509659.239	2136792.926	2096289.243	1975720.322	1987511.178	1930964.641	1959507.247	1443013.164	1791342.104	1577490.58	1730411.895	1913105.861	1873005.785	1835644.821	1696670.518	1777982.63	1583051.562	1490784.268	1871020.931	1495459.956	2584176.265	2563588.93	2716446.495	2517456.041	2669696.843	2504698.856	2613377.006	1982519.495	1756961.766	2270411.781	2341090.637	2433922.458	2493128.442	2600548.767	2466289.083	2614426.68	1779327.068	2234023.088	2541815.055	2462656.655	2371366.575	1409859.972	1747833.671
MS_METABOLITE_DATA_END
#METABOLITES
METABOLITES_START
metabolite_name	m/z	rt
MeAIB_174	174	13.55
Ala_232	232	18.1
Ala_260	260	18.1
Ala_264	264	18.1
METABOLITES_END
#END