#METABOLOMICS WORKBENCH ZHRCzhrc_20250617_041013 DATATRACK_ID:6048 STUDY_ID:ST004023 ANALYSIS_ID:AN006630 PROJECT_ID:PR002516
VERSION             	1
CREATED_ON             	July 6, 2025, 11:29 pm
#PROJECT
PR:PROJECT_TITLE                 	Untargeted Metabolomics Profiling of Human Plasma via LC-Q-TOF for sudden
PR:PROJECT_TITLE                 	sensorineural hearing loss (SSNHL) Biomarker Discovery
PR:PROJECT_TYPE                  	Untargeted Metabolomics
PR:PROJECT_SUMMARY               	This project focuses on untargeted metabolomic analysis of human plasma samples
PR:PROJECT_SUMMARY               	using advanced mass spectrometry techniques (LC-MS). The goal is to (1)
PR:PROJECT_SUMMARY               	characterize the metabolic profile of human plasma; (2) identify potential
PR:PROJECT_SUMMARY               	biomarkers or metabolic pathways associated with sudden sensorineural hearing
PR:PROJECT_SUMMARY               	loss (SSNHL); (3) provide foundational metabolomic data for further biomedical
PR:PROJECT_SUMMARY               	research. Raw data, analytical methods, and metabolite annotations are submitted
PR:PROJECT_SUMMARY               	for transparency and reproducibility. In this study, non-targeted metabolomic
PR:PROJECT_SUMMARY               	analysis revealed that the differentially expressed metabolites in plasma of
PR:PROJECT_SUMMARY               	SSNHL patients mainly focus on lipid and lipid-like molecules, amino acids and
PR:PROJECT_SUMMARY               	their derivatives, flavonoids, as well as organic oxygen compounds involved in
PR:PROJECT_SUMMARY               	central carbon metabolism and energy metabolic pathways such as tricarboxylic
PR:PROJECT_SUMMARY               	acid cycle intermediates.
PR:INSTITUTE                     	The First Affiliated Hospital of Kunming Medical University
PR:LAST_NAME                     	Zhang
PR:FIRST_NAME                    	Huangruici
PR:ADDRESS                       	No. 295 Xichang Road, Kunming, Yunnan, 650032, China
PR:EMAIL                         	1031784375@qq.com
PR:PHONE                         	15508893380
#STUDY
ST:STUDY_TITLE                   	Untargeted Metabolomics Profiling of Human Plasma via LC-Q-TOF for sudden
ST:STUDY_TITLE                   	sensorineural hearing loss (SSNHL) Biomarker Discovery
ST:STUDY_SUMMARY                 	1. Background: Sudden sensorineural hearing loss (SSNHL) is a common otological
ST:STUDY_SUMMARY                 	disorder with complex etiologies and unclear pathophysiology. This study aimed
ST:STUDY_SUMMARY                 	to detect plasma metabolites in SSNHL, identify potential biomarkers, and
ST:STUDY_SUMMARY                 	uncover underlying metabolic mechanisms. 2. Methods: Participants: 64 SSNHL
ST:STUDY_SUMMARY                 	patients and 53 normal controls. Analysis: Plasma samples were analyzed by
ST:STUDY_SUMMARY                 	liquid chromatography-mass spectrometry (LC-MS). Metabolite profiling and
ST:STUDY_SUMMARY                 	multivariate statistical analyses (OPLS-DA, PLS-DA) were performed to screen
ST:STUDY_SUMMARY                 	differentially expressed metabolites. 3. Key Findings: Differential Metabolites:
ST:STUDY_SUMMARY                 	130 metabolites were identified as significantly altered in SSNHL, primarily
ST:STUDY_SUMMARY                 	including: Lipids and lipid-like molecules (e.g., sphingosine), Amino acids and
ST:STUDY_SUMMARY                 	derivatives (e.g., anthranilic acid), Flavonoids (e.g., 6-hydroxyflavanone),
ST:STUDY_SUMMARY                 	Organic oxygen compounds involved in central carbon metabolism. Pathway
ST:STUDY_SUMMARY                 	Analysis: Enriched pathways included central carbon metabolism, protein
ST:STUDY_SUMMARY                 	digestion/absorption, aminoacyl-tRNA biosynthesis, and mineral absorption. 4.
ST:STUDY_SUMMARY                 	Conclusion: Plasma metabolite profiling shows promise as a non-invasive approach
ST:STUDY_SUMMARY                 	for SSNHL biomarker screening. Identified metabolites and pathways provide novel
ST:STUDY_SUMMARY                 	insights into SSNHL pathophysiology, potentially guiding future therapeutic
ST:STUDY_SUMMARY                 	strategies.
ST:INSTITUTE                     	The First Affiliated Hospital of Kunming Medical University
ST:LAST_NAME                     	Zhang
ST:FIRST_NAME                    	Huangruici
ST:ADDRESS                       	No. 295 Xichang Road, Kunming, Yunnan, 650032, China
ST:EMAIL                         	1031784375@qq.com
ST:PHONE                         	15508893380
#SUBJECT
SU:SUBJECT_TYPE                  	Human
SU:SUBJECT_SPECIES               	Homo sapiens
SU:TAXONOMY_ID                   	9606
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data
SUBJECT_SAMPLE_FACTORS           	TX-1	TX-1	Sample source:plasma | Treatment:case	RAW_FILE_NAME(Raw_File_Name)=TX-1.mzXML
SUBJECT_SAMPLE_FACTORS           	TX-2	TX-2	Sample source:plasma | Treatment:case	RAW_FILE_NAME(Raw_File_Name)=TX-2.mzXML
SUBJECT_SAMPLE_FACTORS           	TX-3	TX-3	Sample source:plasma | Treatment:case	RAW_FILE_NAME(Raw_File_Name)=TX-3.mzXML
SUBJECT_SAMPLE_FACTORS           	TX-4	TX-4	Sample source:plasma | Treatment:case	RAW_FILE_NAME(Raw_File_Name)=TX-4.mzXML
SUBJECT_SAMPLE_FACTORS           	TX-5	TX-5	Sample source:plasma | Treatment:case	RAW_FILE_NAME(Raw_File_Name)=TX-5.mzXML
SUBJECT_SAMPLE_FACTORS           	TX-6	TX-6	Sample source:plasma | Treatment:case	RAW_FILE_NAME(Raw_File_Name)=TX-6.mzXML
SUBJECT_SAMPLE_FACTORS           	TX-7	TX-7	Sample source:plasma | Treatment:case	RAW_FILE_NAME(Raw_File_Name)=TX-7.mzXML
SUBJECT_SAMPLE_FACTORS           	TX-8	TX-8	Sample source:plasma | Treatment:case	RAW_FILE_NAME(Raw_File_Name)=TX-8.mzXML
SUBJECT_SAMPLE_FACTORS           	TX-9	TX-9	Sample source:plasma | Treatment:case	RAW_FILE_NAME(Raw_File_Name)=TX-9.mzXML
SUBJECT_SAMPLE_FACTORS           	TX-10	TX-10	Sample source:plasma | Treatment:case	RAW_FILE_NAME(Raw_File_Name)=TX-10.mzXML
SUBJECT_SAMPLE_FACTORS           	TX-11	TX-11	Sample source:plasma | Treatment:case	RAW_FILE_NAME(Raw_File_Name)=TX-11.mzXML
SUBJECT_SAMPLE_FACTORS           	TX-12	TX-12	Sample source:plasma | Treatment:case	RAW_FILE_NAME(Raw_File_Name)=TX-12.mzXML
SUBJECT_SAMPLE_FACTORS           	TX-13	TX-13	Sample source:plasma | Treatment:case	RAW_FILE_NAME(Raw_File_Name)=TX-13.mzXML
SUBJECT_SAMPLE_FACTORS           	TX-14	TX-14	Sample source:plasma | Treatment:case	RAW_FILE_NAME(Raw_File_Name)=TX-14.mzXML
SUBJECT_SAMPLE_FACTORS           	TX-15	TX-15	Sample source:plasma | Treatment:case	RAW_FILE_NAME(Raw_File_Name)=TX-15.mzXML
SUBJECT_SAMPLE_FACTORS           	TX-16	TX-16	Sample source:plasma | Treatment:case	RAW_FILE_NAME(Raw_File_Name)=TX-16.mzXML
SUBJECT_SAMPLE_FACTORS           	TX-17	TX-17	Sample source:plasma | Treatment:case	RAW_FILE_NAME(Raw_File_Name)=TX-17.mzXML
SUBJECT_SAMPLE_FACTORS           	TX-18	TX-18	Sample source:plasma | Treatment:case	RAW_FILE_NAME(Raw_File_Name)=TX-18.mzXML
SUBJECT_SAMPLE_FACTORS           	TX-19	TX-19	Sample source:plasma | Treatment:case	RAW_FILE_NAME(Raw_File_Name)=TX-19.mzXML
SUBJECT_SAMPLE_FACTORS           	TX-20	TX-20	Sample source:plasma | Treatment:case	RAW_FILE_NAME(Raw_File_Name)=TX-20.mzXML
SUBJECT_SAMPLE_FACTORS           	TX-21	TX-21	Sample source:plasma | Treatment:case	RAW_FILE_NAME(Raw_File_Name)=TX-21.mzXML
SUBJECT_SAMPLE_FACTORS           	TX-22	TX-22	Sample source:plasma | Treatment:case	RAW_FILE_NAME(Raw_File_Name)=TX-22.mzXML
SUBJECT_SAMPLE_FACTORS           	TX-23	TX-23	Sample source:plasma | Treatment:case	RAW_FILE_NAME(Raw_File_Name)=TX-23.mzXML
SUBJECT_SAMPLE_FACTORS           	TX-24	TX-24	Sample source:plasma | Treatment:case	RAW_FILE_NAME(Raw_File_Name)=TX-24.mzXML
SUBJECT_SAMPLE_FACTORS           	TX-25	TX-25	Sample source:plasma | Treatment:case	RAW_FILE_NAME(Raw_File_Name)=TX-25.mzXML
SUBJECT_SAMPLE_FACTORS           	TX-26	TX-26	Sample source:plasma | Treatment:case	RAW_FILE_NAME(Raw_File_Name)=TX-26.mzXML
SUBJECT_SAMPLE_FACTORS           	TX-27	TX-27	Sample source:plasma | Treatment:case	RAW_FILE_NAME(Raw_File_Name)=TX-27.mzXML
SUBJECT_SAMPLE_FACTORS           	TX-28	TX-28	Sample source:plasma | Treatment:case	RAW_FILE_NAME(Raw_File_Name)=TX-28.mzXML
SUBJECT_SAMPLE_FACTORS           	TX-29	TX-29	Sample source:plasma | Treatment:case	RAW_FILE_NAME(Raw_File_Name)=TX-29.mzXML
SUBJECT_SAMPLE_FACTORS           	TX-30	TX-30	Sample source:plasma | Treatment:case	RAW_FILE_NAME(Raw_File_Name)=TX-30.mzXML
SUBJECT_SAMPLE_FACTORS           	TX-31	TX-31	Sample source:plasma | Treatment:case	RAW_FILE_NAME(Raw_File_Name)=TX-31.mzXML
SUBJECT_SAMPLE_FACTORS           	TX-32	TX-32	Sample source:plasma | Treatment:case	RAW_FILE_NAME(Raw_File_Name)=TX-32.mzXML
SUBJECT_SAMPLE_FACTORS           	TX-33	TX-33	Sample source:plasma | Treatment:case	RAW_FILE_NAME(Raw_File_Name)=TX-33.mzXML
SUBJECT_SAMPLE_FACTORS           	TX-34	TX-34	Sample source:plasma | Treatment:case	RAW_FILE_NAME(Raw_File_Name)=TX-34.mzXML
SUBJECT_SAMPLE_FACTORS           	TX-35	TX-35	Sample source:plasma | Treatment:case	RAW_FILE_NAME(Raw_File_Name)=TX-35.mzXML
SUBJECT_SAMPLE_FACTORS           	TX-36	TX-36	Sample source:plasma | Treatment:case	RAW_FILE_NAME(Raw_File_Name)=TX-36.mzXML
SUBJECT_SAMPLE_FACTORS           	TX-37	TX-37	Sample source:plasma | Treatment:case	RAW_FILE_NAME(Raw_File_Name)=TX-37.mzXML
SUBJECT_SAMPLE_FACTORS           	TX-38	TX-38	Sample source:plasma | Treatment:case	RAW_FILE_NAME(Raw_File_Name)=TX-38.mzXML
SUBJECT_SAMPLE_FACTORS           	TX-39	TX-39	Sample source:plasma | Treatment:case	RAW_FILE_NAME(Raw_File_Name)=TX-39.mzXML
SUBJECT_SAMPLE_FACTORS           	TX-40	TX-40	Sample source:plasma | Treatment:case	RAW_FILE_NAME(Raw_File_Name)=TX-40.mzXML
SUBJECT_SAMPLE_FACTORS           	TX-41	TX-41	Sample source:plasma | Treatment:case	RAW_FILE_NAME(Raw_File_Name)=TX-41.mzXML
SUBJECT_SAMPLE_FACTORS           	TX-42	TX-42	Sample source:plasma | Treatment:case	RAW_FILE_NAME(Raw_File_Name)=TX-42.mzXML
SUBJECT_SAMPLE_FACTORS           	TX-43	TX-43	Sample source:plasma | Treatment:case	RAW_FILE_NAME(Raw_File_Name)=TX-43.mzXML
SUBJECT_SAMPLE_FACTORS           	TX-44	TX-44	Sample source:plasma | Treatment:case	RAW_FILE_NAME(Raw_File_Name)=TX-44.mzXML
SUBJECT_SAMPLE_FACTORS           	TX-45	TX-45	Sample source:plasma | Treatment:case	RAW_FILE_NAME(Raw_File_Name)=TX-45.mzXML
SUBJECT_SAMPLE_FACTORS           	TX-46	TX-46	Sample source:plasma | Treatment:case	RAW_FILE_NAME(Raw_File_Name)=TX-46.mzXML
SUBJECT_SAMPLE_FACTORS           	TX-47	TX-47	Sample source:plasma | Treatment:case	RAW_FILE_NAME(Raw_File_Name)=TX-47.mzXML
SUBJECT_SAMPLE_FACTORS           	TX-48	TX-48	Sample source:plasma | Treatment:case	RAW_FILE_NAME(Raw_File_Name)=TX-48.mzXML
SUBJECT_SAMPLE_FACTORS           	TX-49	TX-49	Sample source:plasma | Treatment:case	RAW_FILE_NAME(Raw_File_Name)=TX-49.mzXML
SUBJECT_SAMPLE_FACTORS           	TX-50	TX-50	Sample source:plasma | Treatment:case	RAW_FILE_NAME(Raw_File_Name)=TX-50.mzXML
SUBJECT_SAMPLE_FACTORS           	TX-51	TX-51	Sample source:plasma | Treatment:case	RAW_FILE_NAME(Raw_File_Name)=TX-51.mzXML
SUBJECT_SAMPLE_FACTORS           	TX-52	TX-52	Sample source:plasma | Treatment:case	RAW_FILE_NAME(Raw_File_Name)=TX-52.mzXML
SUBJECT_SAMPLE_FACTORS           	TX-53	TX-53	Sample source:plasma | Treatment:case	RAW_FILE_NAME(Raw_File_Name)=TX-53.mzXML
SUBJECT_SAMPLE_FACTORS           	TX-54	TX-54	Sample source:plasma | Treatment:case	RAW_FILE_NAME(Raw_File_Name)=TX-54.mzXML
SUBJECT_SAMPLE_FACTORS           	TX-55	TX-55	Sample source:plasma | Treatment:case	RAW_FILE_NAME(Raw_File_Name)=TX-55.mzXML
SUBJECT_SAMPLE_FACTORS           	TX-56	TX-56	Sample source:plasma | Treatment:case	RAW_FILE_NAME(Raw_File_Name)=TX-56.mzXML
SUBJECT_SAMPLE_FACTORS           	TX-57	TX-57	Sample source:plasma | Treatment:case	RAW_FILE_NAME(Raw_File_Name)=TX-57.mzXML
SUBJECT_SAMPLE_FACTORS           	TX-58	TX-58	Sample source:plasma | Treatment:case	RAW_FILE_NAME(Raw_File_Name)=TX-58.mzXML
SUBJECT_SAMPLE_FACTORS           	TX-59	TX-59	Sample source:plasma | Treatment:case	RAW_FILE_NAME(Raw_File_Name)=TX-59.mzXML
SUBJECT_SAMPLE_FACTORS           	TX-60	TX-60	Sample source:plasma | Treatment:case	RAW_FILE_NAME(Raw_File_Name)=TX-60.mzXML
SUBJECT_SAMPLE_FACTORS           	TX-61	TX-61	Sample source:plasma | Treatment:case	RAW_FILE_NAME(Raw_File_Name)=TX-61.mzXML
SUBJECT_SAMPLE_FACTORS           	TX-62	TX-62	Sample source:plasma | Treatment:case	RAW_FILE_NAME(Raw_File_Name)=TX-62.mzXML
SUBJECT_SAMPLE_FACTORS           	TX-63	TX-63	Sample source:plasma | Treatment:case	RAW_FILE_NAME(Raw_File_Name)=TX-63.mzXML
SUBJECT_SAMPLE_FACTORS           	TX-64	TX-64	Sample source:plasma | Treatment:case	RAW_FILE_NAME(Raw_File_Name)=TX-64.mzXML
SUBJECT_SAMPLE_FACTORS           	CKX-1	CKX-1	Sample source:plasma | Treatment:Control	RAW_FILE_NAME(Raw_File_Name)=CKX-1.mzXML
SUBJECT_SAMPLE_FACTORS           	CKX-2	CKX-2	Sample source:plasma | Treatment:Control	RAW_FILE_NAME(Raw_File_Name)=CKX-2.mzXML
SUBJECT_SAMPLE_FACTORS           	CKX-3	CKX-3	Sample source:plasma | Treatment:Control	RAW_FILE_NAME(Raw_File_Name)=CKX-3.mzXML
SUBJECT_SAMPLE_FACTORS           	CKX-4	CKX-4	Sample source:plasma | Treatment:Control	RAW_FILE_NAME(Raw_File_Name)=CKX-4.mzXML
SUBJECT_SAMPLE_FACTORS           	CKX-5	CKX-5	Sample source:plasma | Treatment:Control	RAW_FILE_NAME(Raw_File_Name)=CKX-5.mzXML
SUBJECT_SAMPLE_FACTORS           	CKX-6	CKX-6	Sample source:plasma | Treatment:Control	RAW_FILE_NAME(Raw_File_Name)=CKX-6.mzXML
SUBJECT_SAMPLE_FACTORS           	CKX-7	CKX-7	Sample source:plasma | Treatment:Control	RAW_FILE_NAME(Raw_File_Name)=CKX-7.mzXML
SUBJECT_SAMPLE_FACTORS           	CKX-8	CKX-8	Sample source:plasma | Treatment:Control	RAW_FILE_NAME(Raw_File_Name)=CKX-8.mzXML
SUBJECT_SAMPLE_FACTORS           	CKX-9	CKX-9	Sample source:plasma | Treatment:Control	RAW_FILE_NAME(Raw_File_Name)=CKX-9.mzXML
SUBJECT_SAMPLE_FACTORS           	CKX-10	CKX-10	Sample source:plasma | Treatment:Control	RAW_FILE_NAME(Raw_File_Name)=CKX-10.mzXML
SUBJECT_SAMPLE_FACTORS           	CKX-11	CKX-11	Sample source:plasma | Treatment:Control	RAW_FILE_NAME(Raw_File_Name)=CKX-11.mzXML
SUBJECT_SAMPLE_FACTORS           	CKX-12	CKX-12	Sample source:plasma | Treatment:Control	RAW_FILE_NAME(Raw_File_Name)=CKX-12.mzXML
SUBJECT_SAMPLE_FACTORS           	CKX-13	CKX-13	Sample source:plasma | Treatment:Control	RAW_FILE_NAME(Raw_File_Name)=CKX-13.mzXML
SUBJECT_SAMPLE_FACTORS           	CKX-14	CKX-14	Sample source:plasma | Treatment:Control	RAW_FILE_NAME(Raw_File_Name)=CKX-14.mzXML
SUBJECT_SAMPLE_FACTORS           	CKX-15	CKX-15	Sample source:plasma | Treatment:Control	RAW_FILE_NAME(Raw_File_Name)=CKX-15.mzXML
SUBJECT_SAMPLE_FACTORS           	CKX-16	CKX-16	Sample source:plasma | Treatment:Control	RAW_FILE_NAME(Raw_File_Name)=CKX-16.mzXML
SUBJECT_SAMPLE_FACTORS           	CKX-17	CKX-17	Sample source:plasma | Treatment:Control	RAW_FILE_NAME(Raw_File_Name)=CKX-17.mzXML
SUBJECT_SAMPLE_FACTORS           	CKX-18	CKX-18	Sample source:plasma | Treatment:Control	RAW_FILE_NAME(Raw_File_Name)=CKX-18.mzXML
SUBJECT_SAMPLE_FACTORS           	CKX-19	CKX-19	Sample source:plasma | Treatment:Control	RAW_FILE_NAME(Raw_File_Name)=CKX-19.mzXML
SUBJECT_SAMPLE_FACTORS           	CKX-20	CKX-20	Sample source:plasma | Treatment:Control	RAW_FILE_NAME(Raw_File_Name)=CKX-20.mzXML
SUBJECT_SAMPLE_FACTORS           	CKX-21	CKX-21	Sample source:plasma | Treatment:Control	RAW_FILE_NAME(Raw_File_Name)=CKX-21.mzXML
SUBJECT_SAMPLE_FACTORS           	CKX-22	CKX-22	Sample source:plasma | Treatment:Control	RAW_FILE_NAME(Raw_File_Name)=CKX-22.mzXML
SUBJECT_SAMPLE_FACTORS           	CKX-23	CKX-23	Sample source:plasma | Treatment:Control	RAW_FILE_NAME(Raw_File_Name)=CKX-23.mzXML
SUBJECT_SAMPLE_FACTORS           	CKX-24	CKX-24	Sample source:plasma | Treatment:Control	RAW_FILE_NAME(Raw_File_Name)=CKX-24.mzXML
SUBJECT_SAMPLE_FACTORS           	CKX-25	CKX-25	Sample source:plasma | Treatment:Control	RAW_FILE_NAME(Raw_File_Name)=CKX-25.mzXML
SUBJECT_SAMPLE_FACTORS           	CKX-26	CKX-26	Sample source:plasma | Treatment:Control	RAW_FILE_NAME(Raw_File_Name)=CKX-26.mzXML
SUBJECT_SAMPLE_FACTORS           	CKX-27	CKX-27	Sample source:plasma | Treatment:Control	RAW_FILE_NAME(Raw_File_Name)=CKX-27.mzXML
SUBJECT_SAMPLE_FACTORS           	CKX-28	CKX-28	Sample source:plasma | Treatment:Control	RAW_FILE_NAME(Raw_File_Name)=CKX-28.mzXML
SUBJECT_SAMPLE_FACTORS           	CKX-29	CKX-29	Sample source:plasma | Treatment:Control	RAW_FILE_NAME(Raw_File_Name)=CKX-29.mzXML
SUBJECT_SAMPLE_FACTORS           	CKX-30	CKX-30	Sample source:plasma | Treatment:Control	RAW_FILE_NAME(Raw_File_Name)=CKX-30.mzXML
SUBJECT_SAMPLE_FACTORS           	CKX-31	CKX-31	Sample source:plasma | Treatment:Control	RAW_FILE_NAME(Raw_File_Name)=CKX-31.mzXML
SUBJECT_SAMPLE_FACTORS           	CKX-32	CKX-32	Sample source:plasma | Treatment:Control	RAW_FILE_NAME(Raw_File_Name)=CKX-32.mzXML
SUBJECT_SAMPLE_FACTORS           	CKX-33	CKX-33	Sample source:plasma | Treatment:Control	RAW_FILE_NAME(Raw_File_Name)=CKX-33.mzXML
SUBJECT_SAMPLE_FACTORS           	CKX-34	CKX-34	Sample source:plasma | Treatment:Control	RAW_FILE_NAME(Raw_File_Name)=CKX-34.mzXML
SUBJECT_SAMPLE_FACTORS           	CKX-35	CKX-35	Sample source:plasma | Treatment:Control	RAW_FILE_NAME(Raw_File_Name)=CKX-35.mzXML
SUBJECT_SAMPLE_FACTORS           	CKX-36	CKX-36	Sample source:plasma | Treatment:Control	RAW_FILE_NAME(Raw_File_Name)=CKX-36.mzXML
SUBJECT_SAMPLE_FACTORS           	CKX-37	CKX-37	Sample source:plasma | Treatment:Control	RAW_FILE_NAME(Raw_File_Name)=CKX-37.mzXML
SUBJECT_SAMPLE_FACTORS           	CKX-38	CKX-38	Sample source:plasma | Treatment:Control	RAW_FILE_NAME(Raw_File_Name)=CKX-38.mzXML
SUBJECT_SAMPLE_FACTORS           	CKX-39	CKX-39	Sample source:plasma | Treatment:Control	RAW_FILE_NAME(Raw_File_Name)=CKX-39.mzXML
SUBJECT_SAMPLE_FACTORS           	CKX-40	CKX-40	Sample source:plasma | Treatment:Control	RAW_FILE_NAME(Raw_File_Name)=CKX-40.mzXML
SUBJECT_SAMPLE_FACTORS           	CKX-41	CKX-41	Sample source:plasma | Treatment:Control	RAW_FILE_NAME(Raw_File_Name)=CKX-41.mzXML
SUBJECT_SAMPLE_FACTORS           	CKX-42	CKX-42	Sample source:plasma | Treatment:Control	RAW_FILE_NAME(Raw_File_Name)=CKX-42.mzXML
SUBJECT_SAMPLE_FACTORS           	CKX-43	CKX-43	Sample source:plasma | Treatment:Control	RAW_FILE_NAME(Raw_File_Name)=CKX-43.mzXML
SUBJECT_SAMPLE_FACTORS           	CKX-44	CKX-44	Sample source:plasma | Treatment:Control	RAW_FILE_NAME(Raw_File_Name)=CKX-44.mzXML
SUBJECT_SAMPLE_FACTORS           	CKX-45	CKX-45	Sample source:plasma | Treatment:Control	RAW_FILE_NAME(Raw_File_Name)=CKX-45.mzXML
SUBJECT_SAMPLE_FACTORS           	CKX-46	CKX-46	Sample source:plasma | Treatment:Control	RAW_FILE_NAME(Raw_File_Name)=CKX-46.mzXML
SUBJECT_SAMPLE_FACTORS           	CKX-47	CKX-47	Sample source:plasma | Treatment:Control	RAW_FILE_NAME(Raw_File_Name)=CKX-47.mzXML
SUBJECT_SAMPLE_FACTORS           	CKX-48	CKX-48	Sample source:plasma | Treatment:Control	RAW_FILE_NAME(Raw_File_Name)=CKX-48.mzXML
SUBJECT_SAMPLE_FACTORS           	CKX-49	CKX-49	Sample source:plasma | Treatment:Control	RAW_FILE_NAME(Raw_File_Name)=CKX-49.mzXML
SUBJECT_SAMPLE_FACTORS           	CKX-50	CKX-50	Sample source:plasma | Treatment:Control	RAW_FILE_NAME(Raw_File_Name)=CKX-50.mzXML
SUBJECT_SAMPLE_FACTORS           	CKX-51	CKX-51	Sample source:plasma | Treatment:Control	RAW_FILE_NAME(Raw_File_Name)=CKX-51.mzXML
SUBJECT_SAMPLE_FACTORS           	CKX-52	CKX-52	Sample source:plasma | Treatment:Control	RAW_FILE_NAME(Raw_File_Name)=CKX-52.mzXML
SUBJECT_SAMPLE_FACTORS           	CKX-53	CKX-53	Sample source:plasma | Treatment:Control	RAW_FILE_NAME(Raw_File_Name)=CKX-53.mzXML
#COLLECTION
CO:COLLECTION_SUMMARY            	Fasting blood samples from control (non-SSNHL) and case (SSNHL) individuals were
CO:COLLECTION_SUMMARY            	collected in 5 mL of vacuum blood collection tube containing EDTA anticoagulant,
CO:COLLECTION_SUMMARY            	centrifuged at 1500g at 4°C for 15 minutes, and 150 μL of plasma were taken
CO:COLLECTION_SUMMARY            	and stored at -80°C. After thawing at 4°C before detection, protein was
CO:COLLECTION_SUMMARY            	precipitated with cold methanol/acetonitrile/water (2:2:1, v/v) and metabolites
CO:COLLECTION_SUMMARY            	were extracted.
CO:COLLECTION_PROTOCOL_FILENAME  	ZHRC_Method.pdf
CO:SAMPLE_TYPE                   	Blood (plasma)
#TREATMENT
TR:TREATMENT_SUMMARY             	No treatment
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	Plasma samples were collected from 64 sudden sensorineural hearing loss (SSNHL)
SP:SAMPLEPREP_SUMMARY            	patients and 53 healthy controls into EDTA-anticoagulated tubes after an
SP:SAMPLEPREP_SUMMARY            	overnight fast. Samples were centrifuged at 1500 × g for 15 min at 4°C, and
SP:SAMPLEPREP_SUMMARY            	150 μL plasma aliquots were stored at –80°C. For metabolomic extraction, 100
SP:SAMPLEPREP_SUMMARY            	μL thawed plasma was mixed with 400 μL cold methanol/acetonitrile (1:1, v/v),
SP:SAMPLEPREP_SUMMARY            	vortexed, sonicated for 30 min at low temperature, and incubated at –20°C for
SP:SAMPLEPREP_SUMMARY            	10 min. The mixture was centrifuged at 14,000 × g for 20 min at 4°C, and the
SP:SAMPLEPREP_SUMMARY            	supernatant was vacuum-dried. The dried pellet was re-dissolved in 100 μL
SP:SAMPLEPREP_SUMMARY            	acetonitrile/water (1:1, v/v), centrifuged again at 14,000 × g for 15 min, and
SP:SAMPLEPREP_SUMMARY            	the supernatant was injected into the UHPLC-MS system. Quality control (QC)
SP:SAMPLEPREP_SUMMARY            	samples were prepared by pooling 10 μL of each plasma sample and inserted every
SP:SAMPLEPREP_SUMMARY            	5 samples to monitor analytical reproducibility, validated via TIC overlap, PCA
SP:SAMPLEPREP_SUMMARY            	clustering, and RSD ≤ 30% for >80% of ion peaks. Total sum normalization was
SP:SAMPLEPREP_SUMMARY            	applied to preprocess data for inter-sample comparability.
SP:SAMPLEPREP_PROTOCOL_FILENAME  	ZHRC_Method.pdf
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_SUMMARY        	Ultra-high performance liquid chromatography (UHPLC) combined with hydrophilic
CH:CHROMATOGRAPHY_SUMMARY        	interaction chromatography (HILIC) for plasma metabolite separation, using
CH:CHROMATOGRAPHY_SUMMARY        	gradient elution and hyphenated with high-resolution mass spectrometry.
CH:CHROMATOGRAPHY_TYPE           	HILIC
CH:INSTRUMENT_NAME               	Agilent 1290 Infinity
CH:COLUMN_NAME                   	Waters ACQUITY UPLC BEH Amide (100 x 2.1mm,1.7um)
CH:SOLVENT_A                     	100% Water; 25 mM ammonium acetate; 25 mM ammonium hydroxide
CH:SOLVENT_B                     	100% Acetonitrile
CH:FLOW_GRADIENT                 	0–1.5 min, 98% B; 1.5–12 min, B decreased to 2%; 12–14 min, hold at 2%;
CH:FLOW_GRADIENT                 	14–14.1 min, B increased to 98%; 14.1–17 min, hold at 98%.
CH:FLOW_RATE                     	0.3 mL/min
CH:COLUMN_TEMPERATURE            	25
CH:METHODS_FILENAME              	ZHRC_Method.pdf
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
AN:ANALYSIS_PROTOCOL_FILE        	ZHRC_Method.pdf
#MS
MS:INSTRUMENT_NAME               	Thermo Q Exactive HF-X Orbitrap
MS:INSTRUMENT_TYPE               	Orbitrap
MS:MS_TYPE                       	ESI
MS:ION_MODE                      	NEGATIVE
MS:MS_COMMENTS                   	High-resolution TOF-MS acquisition for negative ion mode; m/z 60-1000 Da (MS1),
MS:MS_COMMENTS                   	25-1000 Da (MS2); IDA mode with collision energy 35±15 eV; dynamic exclusion of
MS:MS_COMMENTS                   	isotopes (4 Da). Raw data converted to MzXML via ProteoWizard, peak
MS:MS_COMMENTS                   	alignment/extraction by XCMS.
MS:MS_RESULTS_FILE               	ST004023_AN006630_Results.txt	UNITS:Peak area	Has m/z:Yes	Has RT:Yes	RT units:Seconds
#END