#METABOLOMICS WORKBENCH bmarie_20250708_004630 DATATRACK_ID:6144 STUDY_ID:ST004041 ANALYSIS_ID:AN006680 PROJECT_ID:PR002533
VERSION             	1
CREATED_ON             	July 8, 2025, 10:51 pm
#PROJECT
PR:PROJECT_TITLE                 	Spatio-temporal disparities in phytoplankton dynamics and metabolite production
PR:PROJECT_TITLE                 	depending on weather conditions
PR:PROJECT_TYPE                  	Environmental metabolomics
PR:PROJECT_SUMMARY               	Various studies suggest that global change is causing an increase in
PR:PROJECT_SUMMARY               	phytoplankton biomass, cyanobacteria prevalence and cyanotoxin production.
PR:PROJECT_SUMMARY               	However, there are conflicting reports regarding the response of cyanobacteria
PR:PROJECT_SUMMARY               	blooms to global warming and meteorological events, probably because of the lack
PR:PROJECT_SUMMARY               	of global approaches. Metabolomics approaches in natural system hold great
PR:PROJECT_SUMMARY               	promise in investigating the factors leading to variations in phytoplankton
PR:PROJECT_SUMMARY               	successions and subsequent cyanotoxin production. However, eco-metabolomics
PR:PROJECT_SUMMARY               	studies are still scarce in literature and suffer to adequately unravel the
PR:PROJECT_SUMMARY               	biologically relevant variables under environmental changes. In this study, we
PR:PROJECT_SUMMARY               	investigate the temporal and spatial dynamics of phytoplankton community and the
PR:PROJECT_SUMMARY               	production of their primary and secondary untargeted metabolites in response to
PR:PROJECT_SUMMARY               	local meteorological events. Thus, we collected water samples in two points of
PR:PROJECT_SUMMARY               	the Aydat Lake (France): near the inflowing waters from Veyre River and at the
PR:PROJECT_SUMMARY               	middle of the lake during the 2021 summer. Untargeted intracellular metabolites
PR:PROJECT_SUMMARY               	were measured using ultra-high-performance liquid chromatography coupled with a
PR:PROJECT_SUMMARY               	high-resolution mass spectrometer, as well as phytoplankton biovolume and
PR:PROJECT_SUMMARY               	diversity and physicochemical lake’s parameters. Primarily, our results show
PR:PROJECT_SUMMARY               	the increase of the biovolume of diazotrophic cyanobacteria at the end of the
PR:PROJECT_SUMMARY               	drought and after rain events at both sites. During the drought, we observe a
PR:PROJECT_SUMMARY               	strong increase of intracellular lipid contents, probably in response to sudden
PR:PROJECT_SUMMARY               	nitrogen and phosphorus limitation. Differently, during the wet periods, we
PR:PROJECT_SUMMARY               	observe an increase of the phytoplankton glycerophospholipid content, especially
PR:PROJECT_SUMMARY               	at the middle of the lake, whereas significantly higher abundance of secondary
PR:PROJECT_SUMMARY               	metabolites was monitored at site near the wetland area. Some of these
PR:PROJECT_SUMMARY               	metabolites correspond to Cyanopepetids, strongly correlated with the biovolume
PR:PROJECT_SUMMARY               	of Dolichospermum, present at both sites. Therefore, these results suggest
PR:PROJECT_SUMMARY               	adaptative responses to cope with the phytoplankton growing stimulation related
PR:PROJECT_SUMMARY               	with the increase of the nutritive ion influx following the rain events. Our
PR:PROJECT_SUMMARY               	results highlight the existence of local metabolomic niches, as we report
PR:PROJECT_SUMMARY               	significant difference in the intra-cellular content in metabolites between the
PR:PROJECT_SUMMARY               	2 sampling sites, separated by only 200m, while phytoplankton communities were
PR:PROJECT_SUMMARY               	similar.
PR:INSTITUTE                     	Muséum Nationale d'Histoire Naturelle
PR:LAST_NAME                     	Marie
PR:FIRST_NAME                    	Benjamin
PR:ADDRESS                       	12 rue Buffon, Paris, Île-de-france, 75005, France
PR:EMAIL                         	bmarie@mnhn.fr
PR:PHONE                         	01-40-79-32-12
#STUDY
ST:STUDY_TITLE                   	Spatio-temporal disparities in phytoplankton dynamics and metabolite production
ST:STUDY_TITLE                   	depending on weather conditions
ST:STUDY_TYPE                    	Environmental metabolomics
ST:STUDY_SUMMARY                 	Various studies suggest that global change is causing an increase in
ST:STUDY_SUMMARY                 	phytoplankton biomass, cyanobacteria prevalence and cyanotoxin production.
ST:STUDY_SUMMARY                 	However, there are conflicting reports regarding the response of cyanobacteria
ST:STUDY_SUMMARY                 	blooms to global warming and meteorological events, probably because of the lack
ST:STUDY_SUMMARY                 	of global approaches. Metabolomics approaches in natural system hold great
ST:STUDY_SUMMARY                 	promise in investigating the factors leading to variations in phytoplankton
ST:STUDY_SUMMARY                 	successions and subsequent cyanotoxin production. However, eco-metabolomics
ST:STUDY_SUMMARY                 	studies are still scarce in literature and suffer to adequately unravel the
ST:STUDY_SUMMARY                 	biologically relevant variables under environmental changes. In this study, we
ST:STUDY_SUMMARY                 	investigate the temporal and spatial dynamics of phytoplankton community and the
ST:STUDY_SUMMARY                 	production of their primary and secondary untargeted metabolites in response to
ST:STUDY_SUMMARY                 	local meteorological events. Thus, we collected water samples in two points of
ST:STUDY_SUMMARY                 	the Aydat Lake (France): near the inflowing waters from Veyre River and at the
ST:STUDY_SUMMARY                 	middle of the lake during the 2021 summer. Untargeted intracellular metabolites
ST:STUDY_SUMMARY                 	were measured using ultra-high-performance liquid chromatography coupled with a
ST:STUDY_SUMMARY                 	high-resolution mass spectrometer, as well as phytoplankton biovolume and
ST:STUDY_SUMMARY                 	diversity and physicochemical lake’s parameters. Primarily, our results show
ST:STUDY_SUMMARY                 	the increase of the biovolume of diazotrophic cyanobacteria at the end of the
ST:STUDY_SUMMARY                 	drought and after rain events at both sites. During the drought, we observe a
ST:STUDY_SUMMARY                 	strong increase of intracellular lipid contents, probably in response to sudden
ST:STUDY_SUMMARY                 	nitrogen and phosphorus limitation. Differently, during the wet periods, we
ST:STUDY_SUMMARY                 	observe an increase of the phytoplankton glycerophospholipid content, especially
ST:STUDY_SUMMARY                 	at the middle of the lake, whereas significantly higher abundance of secondary
ST:STUDY_SUMMARY                 	metabolites was monitored at site near the wetland area. Some of these
ST:STUDY_SUMMARY                 	metabolites correspond to Cyanopepetides, strongly correlated with the biovolume
ST:STUDY_SUMMARY                 	of Dolichospermum, present at both sites. Therefore, these results suggest
ST:STUDY_SUMMARY                 	adaptative responses to cope with the phytoplankton growing stimulation related
ST:STUDY_SUMMARY                 	with the increase of the nutritive ion influx following the rain events. Our
ST:STUDY_SUMMARY                 	results highlight the existence of local metabolomic niches, as we report
ST:STUDY_SUMMARY                 	significant difference in the intra-cellular content in metabolites between the
ST:STUDY_SUMMARY                 	2 sampling sites, separated by only 200m, while phytoplankton communities were
ST:STUDY_SUMMARY                 	similar.
ST:INSTITUTE                     	Muséum Nationale d'Histoire Naturelle
ST:DEPARTMENT                    	Aviv
ST:LABORATORY                    	UMR 7245 MCAM
ST:LAST_NAME                     	Marie
ST:FIRST_NAME                    	Benjamin
ST:ADDRESS                       	12 rue Buffon, Paris, Île-de-france, 75005, France
ST:EMAIL                         	bmarie@mnhn.fr
ST:PHONE                         	01-40-79-32-12
#SUBJECT
SU:SUBJECT_TYPE                  	Water sample
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data
SUBJECT_SAMPLE_FACTORS           	-	AL0_1_1_1_1409	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:0 | date:23/7	RAW_FILE_NAME(Raw file name)=20220321_AL0_1_1_1_1409.mzXML
SUBJECT_SAMPLE_FACTORS           	-	AL0_2_2_1_1410	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:0 | date:23/7	RAW_FILE_NAME(Raw file name)=20220321_AL0_2_2_1_1410.mzXML
SUBJECT_SAMPLE_FACTORS           	-	AL0_3_3_1_1411	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:0 | date:23/7	RAW_FILE_NAME(Raw file name)=20220321_AL0_3_3_1_1411.mzXML
SUBJECT_SAMPLE_FACTORS           	-	AL0_4_4_1_1412	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:0 | date:23/7	RAW_FILE_NAME(Raw file name)=20220321_AL0_4_4_1_1412.mzXML
SUBJECT_SAMPLE_FACTORS           	-	AL15_1_5_1_1415	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:1.5 | date:23/7	RAW_FILE_NAME(Raw file name)=20220321_AL15_1_5_1_1415.mzXML
SUBJECT_SAMPLE_FACTORS           	-	AL15_2_6_1_1416	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:1.5 | date:23/7	RAW_FILE_NAME(Raw file name)=20220321_AL15_2_6_1_1416.mzXML
SUBJECT_SAMPLE_FACTORS           	-	AL15_3_7_1_1417	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:1.5 | date:23/7	RAW_FILE_NAME(Raw file name)=20220321_AL15_3_7_1_1417.mzXML
SUBJECT_SAMPLE_FACTORS           	-	AL15_4_8_1_1418	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:1.5 | date:23/7	RAW_FILE_NAME(Raw file name)=20220321_AL15_4_8_1_1418.mzXML
SUBJECT_SAMPLE_FACTORS           	-	AZ0_1_9_1_1421	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:0 | date:23/7	RAW_FILE_NAME(Raw file name)=20220321_AZ0_1_9_1_1421.mzXML
SUBJECT_SAMPLE_FACTORS           	-	AZ0_2_10_1_1422	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:0 | date:23/7	RAW_FILE_NAME(Raw file name)=20220321_AZ0_2_10_1_1422.mzXML
SUBJECT_SAMPLE_FACTORS           	-	AZ0_3_11_1_1423	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:0 | date:23/7	RAW_FILE_NAME(Raw file name)=20220321_AZ0_3_11_1_1423.mzXML
SUBJECT_SAMPLE_FACTORS           	-	AZ0_4_12_1_1424	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:0 | date:23/7	RAW_FILE_NAME(Raw file name)=20220321_AZ0_4_12_1_1424.mzXML
SUBJECT_SAMPLE_FACTORS           	-	AZ15_1_13_1_1427	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:1.5 | date:23/7	RAW_FILE_NAME(Raw file name)=20220321_AZ15_1_13_1_1427.mzXML
SUBJECT_SAMPLE_FACTORS           	-	AZ15_2_14_1_1428	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:1.5 | date:23/7	RAW_FILE_NAME(Raw file name)=20220321_AZ15_2_14_1_1428.mzXML
SUBJECT_SAMPLE_FACTORS           	-	AZ15_3_15_1_1429	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:1.5 | date:23/7	RAW_FILE_NAME(Raw file name)=20220321_AZ15_3_15_1_1429.mzXML
SUBJECT_SAMPLE_FACTORS           	-	AZ15_4_16_1_1430	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:1.5 | date:23/7	RAW_FILE_NAME(Raw file name)=20220321_AZ15_4_16_1_1430.mzXML
SUBJECT_SAMPLE_FACTORS           	-	BL0_1_17_1_1433	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:0 | date:26/7	RAW_FILE_NAME(Raw file name)=20220321_BL0_1_17_1_1433.mzXML
SUBJECT_SAMPLE_FACTORS           	-	BL0_2_18_1_1434	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:0 | date:26/7	RAW_FILE_NAME(Raw file name)=20220321_BL0_2_18_1_1434.mzXML
SUBJECT_SAMPLE_FACTORS           	-	BL0_3_19_1_1435	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:0 | date:26/7	RAW_FILE_NAME(Raw file name)=20220321_BL0_3_19_1_1435.mzXML
SUBJECT_SAMPLE_FACTORS           	-	BL0_4_20_1_1436	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:0 | date:26/7	RAW_FILE_NAME(Raw file name)=20220321_BL0_4_20_1_1436.mzXML
SUBJECT_SAMPLE_FACTORS           	-	BL15_1_21_1_1439	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:1.5 | date:26/7	RAW_FILE_NAME(Raw file name)=20220321_BL15_1_21_1_1439.mzXML
SUBJECT_SAMPLE_FACTORS           	-	BL15_2_22_1_1440	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:1.5 | date:26/7	RAW_FILE_NAME(Raw file name)=20220321_BL15_2_22_1_1440.mzXML
SUBJECT_SAMPLE_FACTORS           	-	BL15_3_23_1_1441	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:1.5 | date:26/7	RAW_FILE_NAME(Raw file name)=20220321_BL15_3_23_1_1441.mzXML
SUBJECT_SAMPLE_FACTORS           	-	BL15_4_24_1_1442	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:1.5 | date:26/7	RAW_FILE_NAME(Raw file name)=20220321_BL15_4_24_1_1442.mzXML
SUBJECT_SAMPLE_FACTORS           	-	BZ0_1_25_1_1445	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:0 | date:26/7	RAW_FILE_NAME(Raw file name)=20220321_BZ0_1_25_1_1445.mzXML
SUBJECT_SAMPLE_FACTORS           	-	BZ0_2_26_1_1446	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:0 | date:26/7	RAW_FILE_NAME(Raw file name)=20220321_BZ0_2_26_1_1446.mzXML
SUBJECT_SAMPLE_FACTORS           	-	BZ0_3_27_1_1447	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:0 | date:26/7	RAW_FILE_NAME(Raw file name)=20220321_BZ0_3_27_1_1447.mzXML
SUBJECT_SAMPLE_FACTORS           	-	BZ0_4_28_1_1448	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:0 | date:26/7	RAW_FILE_NAME(Raw file name)=20220321_BZ0_4_28_1_1448.mzXML
SUBJECT_SAMPLE_FACTORS           	-	BZ15_1_29_1_1451	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:1.5 | date:26/7	RAW_FILE_NAME(Raw file name)=20220321_BZ15_1_29_1_1451.mzXML
SUBJECT_SAMPLE_FACTORS           	-	BZ15_2_30_1_1452	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:1.5 | date:26/7	RAW_FILE_NAME(Raw file name)=20220321_BZ15_2_30_1_1452.mzXML
SUBJECT_SAMPLE_FACTORS           	-	BZ15_3_31_1_1453	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:1.5 | date:26/7	RAW_FILE_NAME(Raw file name)=20220321_BZ15_3_31_1_1453.mzXML
SUBJECT_SAMPLE_FACTORS           	-	BZ15_4_32_1_1454	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:1.5 | date:26/7	RAW_FILE_NAME(Raw file name)=20220321_BZ15_4_32_1_1454.mzXML
SUBJECT_SAMPLE_FACTORS           	-	CL0_1_1_1_1467	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:0 | date:28/7	RAW_FILE_NAME(Raw file name)=20220321_CL0_1_1_1_1467.mzXML
SUBJECT_SAMPLE_FACTORS           	-	CL0_2_2_1_1468	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:0 | date:28/7	RAW_FILE_NAME(Raw file name)=20220321_CL0_2_2_1_1468.mzXML
SUBJECT_SAMPLE_FACTORS           	-	CL0_3_3_1_1469	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:0 | date:28/7	RAW_FILE_NAME(Raw file name)=20220321_CL0_3_3_1_1469.mzXML
SUBJECT_SAMPLE_FACTORS           	-	CL0_4_4_1_1470	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:0 | date:28/7	RAW_FILE_NAME(Raw file name)=20220321_CL0_4_4_1_1470.mzXML
SUBJECT_SAMPLE_FACTORS           	-	CL15_1_5_1_1472	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:1.5 | date:28/7	RAW_FILE_NAME(Raw file name)=20220321_CL15_1_5_1_1472.mzXML
SUBJECT_SAMPLE_FACTORS           	-	CL15_2_6_1_1473	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:1.5 | date:28/7	RAW_FILE_NAME(Raw file name)=20220321_CL15_2_6_1_1473.mzXML
SUBJECT_SAMPLE_FACTORS           	-	CL15_3_7_1_1474	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:1.5 | date:28/7	RAW_FILE_NAME(Raw file name)=20220321_CL15_3_7_1_1474.mzXML
SUBJECT_SAMPLE_FACTORS           	-	CL15_4_8_1_1475	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:1.5 | date:28/7	RAW_FILE_NAME(Raw file name)=20220321_CL15_4_8_1_1475.mzXML
SUBJECT_SAMPLE_FACTORS           	-	CZ0_1_49_1_1527	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:0 | date:28/7	RAW_FILE_NAME(Raw file name)=20220321_CZ0_1_49_1_1527.mzXML
SUBJECT_SAMPLE_FACTORS           	-	CZ0_2_50_1_1528	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:0 | date:28/7	RAW_FILE_NAME(Raw file name)=20220321_CZ0_2_50_1_1528.mzXML
SUBJECT_SAMPLE_FACTORS           	-	CZ0_3_51_1_1529	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:0 | date:28/7	RAW_FILE_NAME(Raw file name)=20220321_CZ0_3_51_1_1529.mzXML
SUBJECT_SAMPLE_FACTORS           	-	CZ0_4_52_1_1530	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:0 | date:28/7	RAW_FILE_NAME(Raw file name)=20220321_CZ0_4_52_1_1530.mzXML
SUBJECT_SAMPLE_FACTORS           	-	CZ15_1_53_1_1532	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:1.5 | date:28/7	RAW_FILE_NAME(Raw file name)=20220321_CZ15_1_53_1_1532.mzXML
SUBJECT_SAMPLE_FACTORS           	-	CZ15_2_54_1_1533	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:1.5 | date:28/7	RAW_FILE_NAME(Raw file name)=20220321_CZ15_2_54_1_1533.mzXML
SUBJECT_SAMPLE_FACTORS           	-	CZ15_3_55_1_1534	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:1.5 | date:28/7	RAW_FILE_NAME(Raw file name)=20220321_CZ15_3_55_1_1534.mzXML
SUBJECT_SAMPLE_FACTORS           	-	CZ15_4_56_1_1535	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:1.5 | date:28/7	RAW_FILE_NAME(Raw file name)=20220321_CZ15_4_56_1_1535.mzXML
SUBJECT_SAMPLE_FACTORS           	-	DL0_1_9_1_1477	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:0 | date:2/8	RAW_FILE_NAME(Raw file name)=20220321_DL0_1_9_1_1477.mzXML
SUBJECT_SAMPLE_FACTORS           	-	DL0_2_10_1_1478	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:0 | date:2/8	RAW_FILE_NAME(Raw file name)=20220321_DL0_2_10_1_1478.mzXML
SUBJECT_SAMPLE_FACTORS           	-	DL0_3_11_1_1479	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:0 | date:2/8	RAW_FILE_NAME(Raw file name)=20220321_DL0_3_11_1_1479.mzXML
SUBJECT_SAMPLE_FACTORS           	-	DL0_4_12_1_1480	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:0 | date:2/8	RAW_FILE_NAME(Raw file name)=20220321_DL0_4_12_1_1480.mzXML
SUBJECT_SAMPLE_FACTORS           	-	DL15_1_13_1_1482	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:1.5 | date:2/8	RAW_FILE_NAME(Raw file name)=20220321_DL15_1_13_1_1482.mzXML
SUBJECT_SAMPLE_FACTORS           	-	DL15_2_14_1_1483	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:1.5 | date:2/8	RAW_FILE_NAME(Raw file name)=20220321_DL15_2_14_1_1483.mzXML
SUBJECT_SAMPLE_FACTORS           	-	DL15_3_15_1_1484	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:1.5 | date:2/8	RAW_FILE_NAME(Raw file name)=20220321_DL15_3_15_1_1484.mzXML
SUBJECT_SAMPLE_FACTORS           	-	DL15_4_16_1_1485	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:1.5 | date:2/8	RAW_FILE_NAME(Raw file name)=20220321_DL15_4_16_1_1485.mzXML
SUBJECT_SAMPLE_FACTORS           	-	DZ0_1_57_1_1537	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:0 | date:2/8	RAW_FILE_NAME(Raw file name)=20220321_DZ0_1_57_1_1537.mzXML
SUBJECT_SAMPLE_FACTORS           	-	DZ0_2_58_1_1538	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:0 | date:2/8	RAW_FILE_NAME(Raw file name)=20220321_DZ0_2_58_1_1538.mzXML
SUBJECT_SAMPLE_FACTORS           	-	DZ0_3_59_1_1539	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:0 | date:2/8	RAW_FILE_NAME(Raw file name)=20220321_DZ0_3_59_1_1539.mzXML
SUBJECT_SAMPLE_FACTORS           	-	DZ0_4_60_1_1540	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:0 | date:2/8	RAW_FILE_NAME(Raw file name)=20220321_DZ0_4_60_1_1540.mzXML
SUBJECT_SAMPLE_FACTORS           	-	DZ15_1_61_1_1542	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:1.5 | date:2/8	RAW_FILE_NAME(Raw file name)=20220321_DZ15_1_61_1_1542.mzXML
SUBJECT_SAMPLE_FACTORS           	-	DZ15_2_62_1_1543	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:1.5 | date:2/8	RAW_FILE_NAME(Raw file name)=20220321_DZ15_2_62_1_1543.mzXML
SUBJECT_SAMPLE_FACTORS           	-	DZ15_3_63_1_1544	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:1.5 | date:2/8	RAW_FILE_NAME(Raw file name)=20220321_DZ15_3_63_1_1544.mzXML
SUBJECT_SAMPLE_FACTORS           	-	DZ15_4_64_1_1545	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:1.5 | date:2/8	RAW_FILE_NAME(Raw file name)=20220321_DZ15_4_64_1_1545.mzXML
SUBJECT_SAMPLE_FACTORS           	-	EL0_1_17_1_1487	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:0 | date:5/8	RAW_FILE_NAME(Raw file name)=20220321_EL0_1_17_1_1487.mzXML
SUBJECT_SAMPLE_FACTORS           	-	EL0_2_18_1_1488	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:0 | date:5/8	RAW_FILE_NAME(Raw file name)=20220321_EL0_2_18_1_1488.mzXML
SUBJECT_SAMPLE_FACTORS           	-	EL0_3_19_1_1489	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:0 | date:5/8	RAW_FILE_NAME(Raw file name)=20220321_EL0_3_19_1_1489.mzXML
SUBJECT_SAMPLE_FACTORS           	-	EL0_4_20_1_1490	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:0 | date:5/8	RAW_FILE_NAME(Raw file name)=20220321_EL0_4_20_1_1490.mzXML
SUBJECT_SAMPLE_FACTORS           	-	EL15_1_21_1_1492	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:1.5 | date:5/8	RAW_FILE_NAME(Raw file name)=20220321_EL15_1_21_1_1492.mzXML
SUBJECT_SAMPLE_FACTORS           	-	EL15_2_22_1_1493	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:1.5 | date:5/8	RAW_FILE_NAME(Raw file name)=20220321_EL15_2_22_1_1493.mzXML
SUBJECT_SAMPLE_FACTORS           	-	EL15_3_23_1_1494	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:1.5 | date:5/8	RAW_FILE_NAME(Raw file name)=20220321_EL15_3_23_1_1494.mzXML
SUBJECT_SAMPLE_FACTORS           	-	EL15_4_24_1_1495	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:1.5 | date:5/8	RAW_FILE_NAME(Raw file name)=20220321_EL15_4_24_1_1495.mzXML
SUBJECT_SAMPLE_FACTORS           	-	EZ0_1_65_1_1547	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:0 | date:5/8	RAW_FILE_NAME(Raw file name)=20220321_EZ0_1_65_1_1547.mzXML
SUBJECT_SAMPLE_FACTORS           	-	EZ0_2_66_1_1548	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:0 | date:5/8	RAW_FILE_NAME(Raw file name)=20220321_EZ0_2_66_1_1548.mzXML
SUBJECT_SAMPLE_FACTORS           	-	EZ0_3_67_1_1549	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:0 | date:5/8	RAW_FILE_NAME(Raw file name)=20220321_EZ0_3_67_1_1549.mzXML
SUBJECT_SAMPLE_FACTORS           	-	EZ0_4_68_1_1550	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:0 | date:5/8	RAW_FILE_NAME(Raw file name)=20220321_EZ0_4_68_1_1550.mzXML
SUBJECT_SAMPLE_FACTORS           	-	EZ15_1_69_1_1552	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:1.5 | date:5/8	RAW_FILE_NAME(Raw file name)=20220321_EZ15_1_69_1_1552.mzXML
SUBJECT_SAMPLE_FACTORS           	-	EZ15_2_70_1_1553	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:1.5 | date:5/8	RAW_FILE_NAME(Raw file name)=20220321_EZ15_2_70_1_1553.mzXML
SUBJECT_SAMPLE_FACTORS           	-	EZ15_3_71_1_1554	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:1.5 | date:5/8	RAW_FILE_NAME(Raw file name)=20220321_EZ15_3_71_1_1554.mzXML
SUBJECT_SAMPLE_FACTORS           	-	EZ15_4_72_1_1555	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:1.5 | date:5/8	RAW_FILE_NAME(Raw file name)=20220321_EZ15_4_72_1_1555.mzXML
SUBJECT_SAMPLE_FACTORS           	-	FL0_1_25_1_1497	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:0 | date:9/8	RAW_FILE_NAME(Raw file name)=20220321_FL0_1_25_1_1497.mzXML
SUBJECT_SAMPLE_FACTORS           	-	FL0_2_26_1_1498	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:0 | date:9/8	RAW_FILE_NAME(Raw file name)=20220321_FL0_2_26_1_1498.mzXML
SUBJECT_SAMPLE_FACTORS           	-	FL0_3_27_1_1499	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:0 | date:9/8	RAW_FILE_NAME(Raw file name)=20220321_FL0_3_27_1_1499.mzXML
SUBJECT_SAMPLE_FACTORS           	-	FL0_4_28_1_1500	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:0 | date:9/8	RAW_FILE_NAME(Raw file name)=20220321_FL0_4_28_1_1500.mzXML
SUBJECT_SAMPLE_FACTORS           	-	FL15_1_29_1_1502	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:1.5 | date:9/8	RAW_FILE_NAME(Raw file name)=20220321_FL15_1_29_1_1502.mzXML
SUBJECT_SAMPLE_FACTORS           	-	FL15_2_30_1_1503	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:1.5 | date:9/8	RAW_FILE_NAME(Raw file name)=20220321_FL15_2_30_1_1503.mzXML
SUBJECT_SAMPLE_FACTORS           	-	FL15_3_31_1_1504	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:1.5 | date:9/8	RAW_FILE_NAME(Raw file name)=20220321_FL15_3_31_1_1504.mzXML
SUBJECT_SAMPLE_FACTORS           	-	FL15_4_32_1_1505	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:1.5 | date:9/8	RAW_FILE_NAME(Raw file name)=20220321_FL15_4_32_1_1505.mzXML
SUBJECT_SAMPLE_FACTORS           	-	FZ0_1_73_1_1557	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:0 | date:9/8	RAW_FILE_NAME(Raw file name)=20220321_FZ0_1_73_1_1557.mzXML
SUBJECT_SAMPLE_FACTORS           	-	FZ0_2_74_1_1558	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:0 | date:9/8	RAW_FILE_NAME(Raw file name)=20220321_FZ0_2_74_1_1558.mzXML
SUBJECT_SAMPLE_FACTORS           	-	FZ0_3_75_1_1559	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:0 | date:9/8	RAW_FILE_NAME(Raw file name)=20220321_FZ0_3_75_1_1559.mzXML
SUBJECT_SAMPLE_FACTORS           	-	FZ0_4_76_1_1560	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:0 | date:9/8	RAW_FILE_NAME(Raw file name)=20220321_FZ0_4_76_1_1560.mzXML
SUBJECT_SAMPLE_FACTORS           	-	FZ15_1_77_1_1562	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:1.5 | date:9/8	RAW_FILE_NAME(Raw file name)=20220321_FZ15_1_77_1_1562.mzXML
SUBJECT_SAMPLE_FACTORS           	-	FZ15_2_78_1_1563	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:1.5 | date:9/8	RAW_FILE_NAME(Raw file name)=20220321_FZ15_2_78_1_1563.mzXML
SUBJECT_SAMPLE_FACTORS           	-	FZ15_3_79_1_1564	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:1.5 | date:9/8	RAW_FILE_NAME(Raw file name)=20220321_FZ15_3_79_1_1564.mzXML
SUBJECT_SAMPLE_FACTORS           	-	FZ15_4_80_1_1565	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:1.5 | date:9/8	RAW_FILE_NAME(Raw file name)=20220321_FZ15_4_80_1_1565.mzXML
SUBJECT_SAMPLE_FACTORS           	-	GL0_1_33_1_1507	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:0 | date:16/8	RAW_FILE_NAME(Raw file name)=20220321_GL0_1_33_1_1507.mzXML
SUBJECT_SAMPLE_FACTORS           	-	GL0_2_34_1_1508	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:0 | date:16/8	RAW_FILE_NAME(Raw file name)=20220321_GL0_2_34_1_1508.mzXML
SUBJECT_SAMPLE_FACTORS           	-	GL0_3_35_1_1509	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:0 | date:16/8	RAW_FILE_NAME(Raw file name)=20220321_GL0_3_35_1_1509.mzXML
SUBJECT_SAMPLE_FACTORS           	-	GL0_4_36_1_1510	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:0 | date:16/8	RAW_FILE_NAME(Raw file name)=20220321_GL0_4_36_1_1510.mzXML
SUBJECT_SAMPLE_FACTORS           	-	GL15_1_37_1_1512	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:1.5 | date:16/8	RAW_FILE_NAME(Raw file name)=20220321_GL15_1_37_1_1512.mzXML
SUBJECT_SAMPLE_FACTORS           	-	GL15_2_38_1_1513	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:1.5 | date:16/8	RAW_FILE_NAME(Raw file name)=20220321_GL15_2_38_1_1513.mzXML
SUBJECT_SAMPLE_FACTORS           	-	GL15_3_39_1_1514	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:1.5 | date:16/8	RAW_FILE_NAME(Raw file name)=20220321_GL15_3_39_1_1514.mzXML
SUBJECT_SAMPLE_FACTORS           	-	GL15_4_40_1_1515	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:1.5 | date:16/8	RAW_FILE_NAME(Raw file name)=20220321_GL15_4_40_1_1515.mzXML
SUBJECT_SAMPLE_FACTORS           	-	GZ0_1_81_1_1567	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:0 | date:16/8	RAW_FILE_NAME(Raw file name)=20220321_GZ0_1_81_1_1567.mzXML
SUBJECT_SAMPLE_FACTORS           	-	GZ0_2_82_1_1568	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:0 | date:16/8	RAW_FILE_NAME(Raw file name)=20220321_GZ0_2_82_1_1568.mzXML
SUBJECT_SAMPLE_FACTORS           	-	GZ0_3_83_1_1569	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:0 | date:16/8	RAW_FILE_NAME(Raw file name)=20220321_GZ0_3_83_1_1569.mzXML
SUBJECT_SAMPLE_FACTORS           	-	GZ0_4_84_1_1570	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:0 | date:16/8	RAW_FILE_NAME(Raw file name)=20220321_GZ0_4_84_1_1570.mzXML
SUBJECT_SAMPLE_FACTORS           	-	GZ15_1_85_1_1572	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:1.5 | date:16/8	RAW_FILE_NAME(Raw file name)=20220321_GZ15_1_85_1_1572.mzXML
SUBJECT_SAMPLE_FACTORS           	-	GZ15_2_86_1_1573	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:1.5 | date:16/8	RAW_FILE_NAME(Raw file name)=20220321_GZ15_2_86_1_1573.mzXML
SUBJECT_SAMPLE_FACTORS           	-	GZ15_3_87_1_1574	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:1.5 | date:16/8	RAW_FILE_NAME(Raw file name)=20220321_GZ15_3_87_1_1574.mzXML
SUBJECT_SAMPLE_FACTORS           	-	GZ15_4_88_1_1575	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:1.5 | date:16/8	RAW_FILE_NAME(Raw file name)=20220321_GZ15_4_88_1_1575.mzXML
SUBJECT_SAMPLE_FACTORS           	-	HL0_1_41_1_1517	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:0 | date:23/8	RAW_FILE_NAME(Raw file name)=20220321_HL0_1_41_1_1517.mzXML
SUBJECT_SAMPLE_FACTORS           	-	HL0_2_42_1_1518	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:0 | date:23/8	RAW_FILE_NAME(Raw file name)=20220321_HL0_2_42_1_1518.mzXML
SUBJECT_SAMPLE_FACTORS           	-	HL0_3_43_1_1519	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:0 | date:23/8	RAW_FILE_NAME(Raw file name)=20220321_HL0_3_43_1_1519.mzXML
SUBJECT_SAMPLE_FACTORS           	-	HL0_4_44_1_1520	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:0 | date:23/8	RAW_FILE_NAME(Raw file name)=20220321_HL0_4_44_1_1520.mzXML
SUBJECT_SAMPLE_FACTORS           	-	HL15_1_45_1_1522	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:1.5 | date:23/8	RAW_FILE_NAME(Raw file name)=20220321_HL15_1_45_1_1522.mzXML
SUBJECT_SAMPLE_FACTORS           	-	HL15_2_46_1_1523	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:1.5 | date:23/8	RAW_FILE_NAME(Raw file name)=20220321_HL15_2_46_1_1523.mzXML
SUBJECT_SAMPLE_FACTORS           	-	HL15_3_47_1_1524	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:1.5 | date:23/8	RAW_FILE_NAME(Raw file name)=20220321_HL15_3_47_1_1524.mzXML
SUBJECT_SAMPLE_FACTORS           	-	HL15_4_48_1_1525	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:1.5 | date:23/8	RAW_FILE_NAME(Raw file name)=20220321_HL15_4_48_1_1525.mzXML
SUBJECT_SAMPLE_FACTORS           	-	HZ0_1_89_1_1577	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:0 | date:23/8	RAW_FILE_NAME(Raw file name)=20220321_HZ0_1_89_1_1577.mzXML
SUBJECT_SAMPLE_FACTORS           	-	HZ0_2_90_1_1578	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:0 | date:23/8	RAW_FILE_NAME(Raw file name)=20220321_HZ0_2_90_1_1578.mzXML
SUBJECT_SAMPLE_FACTORS           	-	HZ0_3_91_1_1579	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:0 | date:23/8	RAW_FILE_NAME(Raw file name)=20220321_HZ0_3_91_1_1579.mzXML
SUBJECT_SAMPLE_FACTORS           	-	HZ0_4_92_1_1580	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:0 | date:23/8	RAW_FILE_NAME(Raw file name)=20220321_HZ0_4_92_1_1580.mzXML
SUBJECT_SAMPLE_FACTORS           	-	HZ15_1_93_1_1582	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:1.5 | date:23/8	RAW_FILE_NAME(Raw file name)=20220321_HZ15_1_93_1_1582.mzXML
SUBJECT_SAMPLE_FACTORS           	-	HZ15_2_94_1_1583	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:1.5 | date:23/8	RAW_FILE_NAME(Raw file name)=20220321_HZ15_2_94_1_1583.mzXML
SUBJECT_SAMPLE_FACTORS           	-	HZ15_3_95_1_1584	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:1.5 | date:23/8	RAW_FILE_NAME(Raw file name)=20220321_HZ15_3_95_1_1584.mzXML
SUBJECT_SAMPLE_FACTORS           	-	HZ15_4_96_1_1585	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:1.5 | date:23/8	RAW_FILE_NAME(Raw file name)=20220321_HZ15_4_96_1_1585.mzXML
SUBJECT_SAMPLE_FACTORS           	-	IL0_1_1_1_1587	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:0 | date:30/8	RAW_FILE_NAME(Raw file name)=20220321_IL0_1_1_1_1587.mzXML
SUBJECT_SAMPLE_FACTORS           	-	IL0_2_2_1_1588	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:0 | date:30/8	RAW_FILE_NAME(Raw file name)=20220321_IL0_2_2_1_1588.mzXML
SUBJECT_SAMPLE_FACTORS           	-	IL0_3_3_1_1589	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:0 | date:30/8	RAW_FILE_NAME(Raw file name)=20220321_IL0_3_3_1_1589.mzXML
SUBJECT_SAMPLE_FACTORS           	-	IL0_4_4_1_1590	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:0 | date:30/8	RAW_FILE_NAME(Raw file name)=20220321_IL0_4_4_1_1590.mzXML
SUBJECT_SAMPLE_FACTORS           	-	IL15_1_5_1_1592	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:1.5 | date:30/8	RAW_FILE_NAME(Raw file name)=20220321_IL15_1_5_1_1592.mzXML
SUBJECT_SAMPLE_FACTORS           	-	IL15_2_6_1_1593	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:1.5 | date:30/8	RAW_FILE_NAME(Raw file name)=20220321_IL15_2_6_1_1593.mzXML
SUBJECT_SAMPLE_FACTORS           	-	IL15_3_7_1_1594	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:1.5 | date:30/8	RAW_FILE_NAME(Raw file name)=20220321_IL15_3_7_1_1594.mzXML
SUBJECT_SAMPLE_FACTORS           	-	IL15_4_8_1_1595	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:1.5 | date:30/8	RAW_FILE_NAME(Raw file name)=20220321_IL15_4_8_1_1595.mzXML
SUBJECT_SAMPLE_FACTORS           	-	IZ0_1_1_1_2114	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:0 | date:30/8	RAW_FILE_NAME(Raw file name)=20220407_IZ0_1_1_1_2114.mzXML
SUBJECT_SAMPLE_FACTORS           	-	IZ0_2_2_1_2115	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:0 | date:30/8	RAW_FILE_NAME(Raw file name)=20220407_IZ0_2_2_1_2115.mzXML
SUBJECT_SAMPLE_FACTORS           	-	IZ0_3_3_1_2116	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:0 | date:30/8	RAW_FILE_NAME(Raw file name)=20220407_IZ0_3_3_1_2116.mzXML
SUBJECT_SAMPLE_FACTORS           	-	IZ0_4_4_1_2117	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:0 | date:30/8	RAW_FILE_NAME(Raw file name)=20220407_IZ0_4_4_1_2117.mzXML
SUBJECT_SAMPLE_FACTORS           	-	IZ15_1_5_1_2119	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:1.5 | date:30/8	RAW_FILE_NAME(Raw file name)=20220407_IZ15_1_5_1_2119.mzXML
SUBJECT_SAMPLE_FACTORS           	-	IZ15_2_6_1_2120	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:1.5 | date:30/8	RAW_FILE_NAME(Raw file name)=20220407_IZ15_2_6_1_2120.mzXML
SUBJECT_SAMPLE_FACTORS           	-	IZ15_3_7_1_2121	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:1.5 | date:30/8	RAW_FILE_NAME(Raw file name)=20220407_IZ15_3_7_1_2121.mzXML
SUBJECT_SAMPLE_FACTORS           	-	IZ15_4_8_1_2122	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:1.5 | date:30/8	RAW_FILE_NAME(Raw file name)=20220407_IZ15_4_8_1_2122.mzXML
SUBJECT_SAMPLE_FACTORS           	-	JL0_1_9_1_1597	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:0 | date:6/9	RAW_FILE_NAME(Raw file name)=20220321_JL0_1_9_1_1597.mzXML
SUBJECT_SAMPLE_FACTORS           	-	JL0_2_10_1_1598	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:0 | date:6/9	RAW_FILE_NAME(Raw file name)=20220321_JL0_2_10_1_1598.mzXML
SUBJECT_SAMPLE_FACTORS           	-	JL0_3_11_1_1599	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:0 | date:6/9	RAW_FILE_NAME(Raw file name)=20220321_JL0_3_11_1_1599.mzXML
SUBJECT_SAMPLE_FACTORS           	-	JL0_4_12_1_1600	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:0 | date:6/9	RAW_FILE_NAME(Raw file name)=20220321_JL0_4_12_1_1600.mzXML
SUBJECT_SAMPLE_FACTORS           	-	JL15_1_13_1_1602	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:1.5 | date:6/9	RAW_FILE_NAME(Raw file name)=20220321_JL15_1_13_1_1602.mzXML
SUBJECT_SAMPLE_FACTORS           	-	JL15_2_14_1_1603	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:1.5 | date:6/9	RAW_FILE_NAME(Raw file name)=20220321_JL15_2_14_1_1603.mzXML
SUBJECT_SAMPLE_FACTORS           	-	JL15_3_15_1_1604	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:1.5 | date:6/9	RAW_FILE_NAME(Raw file name)=20220321_JL15_3_15_1_1604.mzXML
SUBJECT_SAMPLE_FACTORS           	-	JL15_4_16_1_1605	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:1.5 | date:6/9	RAW_FILE_NAME(Raw file name)=20220321_JL15_4_16_1_1605.mzXML
SUBJECT_SAMPLE_FACTORS           	-	JZ0_1_9_1_2124	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:0 | date:6/9	RAW_FILE_NAME(Raw file name)=20220407_JZ0_1_9_1_2124.mzXML
SUBJECT_SAMPLE_FACTORS           	-	JZ0_2_10_1_2125	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:0 | date:6/9	RAW_FILE_NAME(Raw file name)=20220407_JZ0_2_10_1_2125.mzXML
SUBJECT_SAMPLE_FACTORS           	-	JZ0_3_11_1_2126	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:0 | date:6/9	RAW_FILE_NAME(Raw file name)=20220407_JZ0_3_11_1_2126.mzXML
SUBJECT_SAMPLE_FACTORS           	-	JZ0_4_12_1_2127	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:0 | date:6/9	RAW_FILE_NAME(Raw file name)=20220407_JZ0_4_12_1_2127.mzXML
SUBJECT_SAMPLE_FACTORS           	-	JZ15_1_13_1_2129	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:1.5 | date:6/9	RAW_FILE_NAME(Raw file name)=20220407_JZ15_1_13_1_2129.mzXML
SUBJECT_SAMPLE_FACTORS           	-	JZ15_2_14_1_2130	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:1.5 | date:6/9	RAW_FILE_NAME(Raw file name)=20220407_JZ15_2_14_1_2130.mzXML
SUBJECT_SAMPLE_FACTORS           	-	JZ15_3_15_1_2131	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:1.5 | date:6/9	RAW_FILE_NAME(Raw file name)=20220407_JZ15_3_15_1_2131.mzXML
SUBJECT_SAMPLE_FACTORS           	-	JZ15_4_16_1_2132	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:1.5 | date:6/9	RAW_FILE_NAME(Raw file name)=20220407_JZ15_4_16_1_2132.mzXML
SUBJECT_SAMPLE_FACTORS           	-	KL0_1_17_1_1607	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:0 | date:13/9	RAW_FILE_NAME(Raw file name)=20220321_KL0_1_17_1_1607.mzXML
SUBJECT_SAMPLE_FACTORS           	-	KL0_2_18_1_1608	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:0 | date:13/9	RAW_FILE_NAME(Raw file name)=20220321_KL0_2_18_1_1608.mzXML
SUBJECT_SAMPLE_FACTORS           	-	KL0_3_19_1_1609	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:0 | date:13/9	RAW_FILE_NAME(Raw file name)=20220321_KL0_3_19_1_1609.mzXML
SUBJECT_SAMPLE_FACTORS           	-	KL0_4_20_1_1610	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:0 | date:13/9	RAW_FILE_NAME(Raw file name)=20220321_KL0_4_20_1_1610.mzXML
SUBJECT_SAMPLE_FACTORS           	-	KL15_1_21_1_1612	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:1.5 | date:13/9	RAW_FILE_NAME(Raw file name)=20220321_KL15_1_21_1_1612.mzXML
SUBJECT_SAMPLE_FACTORS           	-	KL15_2_22_1_1613	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:1.5 | date:13/9	RAW_FILE_NAME(Raw file name)=20220321_KL15_2_22_1_1613.mzXML
SUBJECT_SAMPLE_FACTORS           	-	KL15_3_23_1_1614	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:1.5 | date:13/9	RAW_FILE_NAME(Raw file name)=20220321_KL15_3_23_1_1614.mzXML
SUBJECT_SAMPLE_FACTORS           	-	KL15_4_24_1_1615	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:1.5 | date:13/9	RAW_FILE_NAME(Raw file name)=20220321_KL15_4_24_1_1615.mzXML
SUBJECT_SAMPLE_FACTORS           	-	KZ0_1_17_1_2134	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:0 | date:13/9	RAW_FILE_NAME(Raw file name)=20220407_KZ0_1_17_1_2134.mzXML
SUBJECT_SAMPLE_FACTORS           	-	KZ0_2_18_1_2135	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:0 | date:13/9	RAW_FILE_NAME(Raw file name)=20220407_KZ0_2_18_1_2135.mzXML
SUBJECT_SAMPLE_FACTORS           	-	KZ0_3_19_1_2136	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:0 | date:13/9	RAW_FILE_NAME(Raw file name)=20220407_KZ0_3_19_1_2136.mzXML
SUBJECT_SAMPLE_FACTORS           	-	KZ0_4_20_1_2137	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:0 | date:13/9	RAW_FILE_NAME(Raw file name)=20220407_KZ0_4_20_1_2137.mzXML
SUBJECT_SAMPLE_FACTORS           	-	KZ15_1_21_1_2139	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:1.5 | date:13/9	RAW_FILE_NAME(Raw file name)=20220407_KZ15_1_21_1_2139.mzXML
SUBJECT_SAMPLE_FACTORS           	-	KZ15_2_22_1_2140	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:1.5 | date:13/9	RAW_FILE_NAME(Raw file name)=20220407_KZ15_2_22_1_2140.mzXML
SUBJECT_SAMPLE_FACTORS           	-	KZ15_3_23_1_2141	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:1.5 | date:13/9	RAW_FILE_NAME(Raw file name)=20220407_KZ15_3_23_1_2141.mzXML
SUBJECT_SAMPLE_FACTORS           	-	KZ15_4_24_1_2142	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:1.5 | date:13/9	RAW_FILE_NAME(Raw file name)=20220407_KZ15_4_24_1_2142.mzXML
SUBJECT_SAMPLE_FACTORS           	-	LL0_1_25_1_1617	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:0 | date:17/9	RAW_FILE_NAME(Raw file name)=20220321_LL0_1_25_1_1617.mzXML
SUBJECT_SAMPLE_FACTORS           	-	LL0_2_26_1_1618	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:0 | date:17/9	RAW_FILE_NAME(Raw file name)=20220321_LL0_2_26_1_1618.mzXML
SUBJECT_SAMPLE_FACTORS           	-	LL0_3_27_1_1619	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:0 | date:17/9	RAW_FILE_NAME(Raw file name)=20220321_LL0_3_27_1_1619.mzXML
SUBJECT_SAMPLE_FACTORS           	-	LL0_4_28_1_1620	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:0 | date:17/9	RAW_FILE_NAME(Raw file name)=20220321_LL0_4_28_1_1620.mzXML
SUBJECT_SAMPLE_FACTORS           	-	LL15_1_29_1_1622	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:1.5 | date:17/9	RAW_FILE_NAME(Raw file name)=20220321_LL15_1_29_1_1622.mzXML
SUBJECT_SAMPLE_FACTORS           	-	LL15_2_30_1_1623	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:1.5 | date:17/9	RAW_FILE_NAME(Raw file name)=20220321_LL15_2_30_1_1623.mzXML
SUBJECT_SAMPLE_FACTORS           	-	LL15_3_31_1_1624	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:1.5 | date:17/9	RAW_FILE_NAME(Raw file name)=20220321_LL15_3_31_1_1624.mzXML
SUBJECT_SAMPLE_FACTORS           	-	LL15_4_32_1_1625	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:1.5 | date:17/9	RAW_FILE_NAME(Raw file name)=20220321_LL15_4_32_1_1625.mzXML
SUBJECT_SAMPLE_FACTORS           	-	LZ0_1_25_1_2144	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:0 | date:17/9	RAW_FILE_NAME(Raw file name)=20220407_LZ0_1_25_1_2144.mzXML
SUBJECT_SAMPLE_FACTORS           	-	LZ0_2_26_1_2145	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:0 | date:17/9	RAW_FILE_NAME(Raw file name)=20220407_LZ0_2_26_1_2145.mzXML
SUBJECT_SAMPLE_FACTORS           	-	LZ0_3_27_1_2146	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:0 | date:17/9	RAW_FILE_NAME(Raw file name)=20220407_LZ0_3_27_1_2146.mzXML
SUBJECT_SAMPLE_FACTORS           	-	LZ0_4_28_1_2147	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:0 | date:17/9	RAW_FILE_NAME(Raw file name)=20220407_LZ0_4_28_1_2147.mzXML
SUBJECT_SAMPLE_FACTORS           	-	LZ15_1_29_1_2149	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:1.5 | date:17/9	RAW_FILE_NAME(Raw file name)=20220407_LZ15_1_29_1_2149.mzXML
SUBJECT_SAMPLE_FACTORS           	-	LZ15_2_30_1_2150	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:1.5 | date:17/9	RAW_FILE_NAME(Raw file name)=20220407_LZ15_2_30_1_2150.mzXML
SUBJECT_SAMPLE_FACTORS           	-	LZ15_3_31_1_2151	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:1.5 | date:17/9	RAW_FILE_NAME(Raw file name)=20220407_LZ15_3_31_1_2151.mzXML
SUBJECT_SAMPLE_FACTORS           	-	LZ15_4_32_1_2152	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:1.5 | date:17/9	RAW_FILE_NAME(Raw file name)=20220407_LZ15_4_32_1_2152.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ML0_1_33_1_1627	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:0 | date:23/9	RAW_FILE_NAME(Raw file name)=20220321_ML0_1_33_1_1627.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ML0_2_34_1_1628	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:0 | date:23/9	RAW_FILE_NAME(Raw file name)=20220321_ML0_2_34_1_1628.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ML0_3_35_1_1629	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:0 | date:23/9	RAW_FILE_NAME(Raw file name)=20220321_ML0_3_35_1_1629.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ML0_4_36_1_1630	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:0 | date:23/9	RAW_FILE_NAME(Raw file name)=20220321_ML0_4_36_1_1630.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ML15_1_37_1_1632	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:1.5 | date:23/9	RAW_FILE_NAME(Raw file name)=20220321_ML15_1_37_1_1632.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ML15_2_38_1_1633	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:1.5 | date:23/9	RAW_FILE_NAME(Raw file name)=20220321_ML15_2_38_1_1633.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ML15_3_39_1_1634	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:1.5 | date:23/9	RAW_FILE_NAME(Raw file name)=20220321_ML15_3_39_1_1634.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ML15_4_40_1_1635	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:1.5 | date:23/9	RAW_FILE_NAME(Raw file name)=20220321_ML15_4_40_1_1635.mzXML
SUBJECT_SAMPLE_FACTORS           	-	MZ0_1_33_1_2154	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:0 | date:23/9	RAW_FILE_NAME(Raw file name)=20220407_MZ0_1_33_1_2154.mzXML
SUBJECT_SAMPLE_FACTORS           	-	MZ0_2_34_1_2155	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:0 | date:23/9	RAW_FILE_NAME(Raw file name)=20220407_MZ0_2_34_1_2155.mzXML
SUBJECT_SAMPLE_FACTORS           	-	MZ0_3_35_1_2156	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:0 | date:23/9	RAW_FILE_NAME(Raw file name)=20220407_MZ0_3_35_1_2156.mzXML
SUBJECT_SAMPLE_FACTORS           	-	MZ0_4_36_1_2157	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:0 | date:23/9	RAW_FILE_NAME(Raw file name)=20220407_MZ0_4_36_1_2157.mzXML
SUBJECT_SAMPLE_FACTORS           	-	MZ15_1_37_1_2159	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:1.5 | date:23/9	RAW_FILE_NAME(Raw file name)=20220407_MZ15_1_37_1_2159.mzXML
SUBJECT_SAMPLE_FACTORS           	-	MZ15_2_38_1_2160	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:1.5 | date:23/9	RAW_FILE_NAME(Raw file name)=20220407_MZ15_2_38_1_2160.mzXML
SUBJECT_SAMPLE_FACTORS           	-	MZ15_3_39_1_2161	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:1.5 | date:23/9	RAW_FILE_NAME(Raw file name)=20220407_MZ15_3_39_1_2161.mzXML
SUBJECT_SAMPLE_FACTORS           	-	MZ15_4_40_1_2162	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:1.5 | date:23/9	RAW_FILE_NAME(Raw file name)=20220407_MZ15_4_40_1_2162.mzXML
SUBJECT_SAMPLE_FACTORS           	-	NL0_1_41_1_1637	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:0 | date:27/9	RAW_FILE_NAME(Raw file name)=20220321_NL0_1_41_1_1637.mzXML
SUBJECT_SAMPLE_FACTORS           	-	NL0_2_42_1_1638	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:0 | date:27/9	RAW_FILE_NAME(Raw file name)=20220321_NL0_2_42_1_1638.mzXML
SUBJECT_SAMPLE_FACTORS           	-	NL0_3_43_1_1639	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:0 | date:27/9	RAW_FILE_NAME(Raw file name)=20220321_NL0_3_43_1_1639.mzXML
SUBJECT_SAMPLE_FACTORS           	-	NL0_4_44_1_1640	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:0 | date:27/9	RAW_FILE_NAME(Raw file name)=20220321_NL0_4_44_1_1640.mzXML
SUBJECT_SAMPLE_FACTORS           	-	NL15_1_45_1_1642	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:1.5 | date:27/9	RAW_FILE_NAME(Raw file name)=20220321_NL15_1_45_1_1642.mzXML
SUBJECT_SAMPLE_FACTORS           	-	NL15_2_46_1_1643	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:1.5 | date:27/9	RAW_FILE_NAME(Raw file name)=20220321_NL15_2_46_1_1643.mzXML
SUBJECT_SAMPLE_FACTORS           	-	NL15_3_47_1_1644	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:1.5 | date:27/9	RAW_FILE_NAME(Raw file name)=20220321_NL15_3_47_1_1644.mzXML
SUBJECT_SAMPLE_FACTORS           	-	NL15_4_48_1_1645	Sample source:Filtered lake water | Site (Z inflow L middle lake):L | profondeur:1.5 | date:27/9	RAW_FILE_NAME(Raw file name)=20220321_NL15_4_48_1_1645.mzXML
SUBJECT_SAMPLE_FACTORS           	-	NZ0_1_41_1_2164	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:0 | date:27/9	RAW_FILE_NAME(Raw file name)=20220407_NZ0_1_41_1_2164.mzXML
SUBJECT_SAMPLE_FACTORS           	-	NZ0_2_42_1_2165	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:0 | date:27/9	RAW_FILE_NAME(Raw file name)=20220407_NZ0_2_42_1_2165.mzXML
SUBJECT_SAMPLE_FACTORS           	-	NZ0_3_43_1_2166	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:0 | date:27/9	RAW_FILE_NAME(Raw file name)=20220407_NZ0_3_43_1_2166.mzXML
SUBJECT_SAMPLE_FACTORS           	-	NZ0_4_44_1_2167	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:0 | date:27/9	RAW_FILE_NAME(Raw file name)=20220407_NZ0_4_44_1_2167.mzXML
SUBJECT_SAMPLE_FACTORS           	-	NZ15_1_45_1_2169	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:1.5 | date:27/9	RAW_FILE_NAME(Raw file name)=20220407_NZ15_1_45_1_2169.mzXML
SUBJECT_SAMPLE_FACTORS           	-	NZ15_2_46_1_2170	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:1.5 | date:27/9	RAW_FILE_NAME(Raw file name)=20220407_NZ15_2_46_1_2170.mzXML
SUBJECT_SAMPLE_FACTORS           	-	NZ15_3_47_1_2171	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:1.5 | date:27/9	RAW_FILE_NAME(Raw file name)=20220407_NZ15_3_47_1_2171.mzXML
SUBJECT_SAMPLE_FACTORS           	-	NZ15_4_48_1_2172	Sample source:Filtered lake water | Site (Z inflow L middle lake):Z | profondeur:1.5 | date:27/9	RAW_FILE_NAME(Raw file name)=20220407_NZ15_4_48_1_2172.mzXML
#COLLECTION
CO:COLLECTION_SUMMARY            	Lake Aydat (45.6°N; 2.9°E) is in the French Massif Central, around 15 km
CO:COLLECTION_SUMMARY            	southwest of Clermont Ferrand, at 837 m above sea level. The monitoring of Lake
CO:COLLECTION_SUMMARY            	Aydat has been conducted from August 05th, 2021, to September 27th, 2021. Weekly
CO:COLLECTION_SUMMARY            	and sporadic surface lake water samples are taken both prior to and following
CO:COLLECTION_SUMMARY            	precipitation events. At each depth and location, approximately 10 liters of
CO:COLLECTION_SUMMARY            	lake water collected with a Van Dorn bottle are then pre-filtered through a
CO:COLLECTION_SUMMARY            	250-micrometer Nylon filter to remove larger particles and kept phytoplankton,
CO:COLLECTION_SUMMARY            	and then transferred into 15-liter containers. The lake water is immediately
CO:COLLECTION_SUMMARY            	filtered in the laboratory using a 150-μm Nylon membrane to avoid the presence
CO:COLLECTION_SUMMARY            	of zooplankton in the lake samples. For each depth and location, one liter of
CO:COLLECTION_SUMMARY            	lake water is filtered on 10-μm nylon membranes using ultra-fast filtration and
CO:COLLECTION_SUMMARY            	then are immediately transferred into 15 mL sterile falcon tubes and quickly
CO:COLLECTION_SUMMARY            	flash-frozen in liquid nitrogen to preserve the samples for metabolomics
CO:COLLECTION_SUMMARY            	analysis.
CO:SAMPLE_TYPE                   	Algae
CO:COLLECTION_LOCATION           	Aydat lake (France)
CO:COLLECTION_FREQUENCY          	Weekly
CO:COLLECTION_DURATION           	2 month
CO:VOLUMEORAMOUNT_COLLECTED      	10 L
CO:STORAGE_CONDITIONS            	-20℃
#TREATMENT
TR:TREATMENT_SUMMARY             	For each depth and location, one liter of lake water is filtered on 10-μm nylon
TR:TREATMENT_SUMMARY             	membranes using ultra-fast filtration and then are immediately transferred into
TR:TREATMENT_SUMMARY             	15 mL sterile falcon tubes and quickly flash-frozen in liquid nitrogen to
TR:TREATMENT_SUMMARY             	preserve the samples for metabolomics analysis. This process, known as
TR:TREATMENT_SUMMARY             	quenching, is performed as quickly as possible to ensure a reliable method and
TR:TREATMENT_SUMMARY             	minimize any disturbance to the endo-metabolism of cells. The filtration time
TR:TREATMENT_SUMMARY             	for each sample is less than 30 seconds, and the Falcon tubes are kept at -20°C
TR:TREATMENT_SUMMARY             	until the day of extraction. This procedure is applied four times to ensure four
TR:TREATMENT_SUMMARY             	replicates of each sample per site and depth. Blanks are performed by filtering
TR:TREATMENT_SUMMARY             	1 liter of sterile ultra-pure water.
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	On the day of extraction, we rinse the filter with 1 mL of sterile MQ water
SP:SAMPLEPREP_SUMMARY            	mixed with 0.1% NaCl and then vortex it. The filter is then centrifuged at 4°C
SP:SAMPLEPREP_SUMMARY            	(5,000 g, 5 min) with the Falcon tube cap attached, and we transfer the pellet
SP:SAMPLEPREP_SUMMARY            	to an Eppendorf tube. The Eppendorf tube is centrifuged at 4°C (15000 g, 10
SP:SAMPLEPREP_SUMMARY            	min) and the supernatant is discarded, allowing us to weigh the pellet. We
SP:SAMPLEPREP_SUMMARY            	adjust the volume of an ice-cold solvent mixture, composed of methanol,
SP:SAMPLEPREP_SUMMARY            	acetonitrile, and water in a 2:2:1 ratio, according to the weighed biomass: 100
SP:SAMPLEPREP_SUMMARY            	μL of the ice-cold solvent mixture corresponds to 1 mg of biomass. For the
SP:SAMPLEPREP_SUMMARY            	blank, we systematically add 200 μL of the mixture. Next, we subject the
SP:SAMPLEPREP_SUMMARY            	samples to mechanical disruption through three cycles of freezing and thawing
SP:SAMPLEPREP_SUMMARY            	using liquid nitrogen, alternating with three cycles of sonication. Each
SP:SAMPLEPREP_SUMMARY            	sonication cycle lasts 15 seconds and is performed at a power of 40% using a
SP:SAMPLEPREP_SUMMARY            	Fisherbrand™ Model 120 Sonic Dismembrator. Throughout this process, we keep
SP:SAMPLEPREP_SUMMARY            	the samples in ice. After extraction, the samples are centrifuged at 4°C (15000
SP:SAMPLEPREP_SUMMARY            	g, 10 min) before being transferred into 40 μL vials (AR0-9973-13, Verex™
SP:SAMPLEPREP_SUMMARY            	Vial Kit) and stored at -80°C until the detection of metabolites. In total, we
SP:SAMPLEPREP_SUMMARY            	extract 224 samples through four serial extractions, with a blank sample added
SP:SAMPLEPREP_SUMMARY            	systematically in each serial.
SP:PROCESSING_STORAGE_CONDITIONS 	-80℃
SP:EXTRACT_STORAGE               	On ice
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_SUMMARY        	The extracts are further analyzed by injecting 2 μL of the solution onto a C18
CH:CHROMATOGRAPHY_SUMMARY        	column (Polar Advantage II 2.5 pore, Thermo) using an ultra-high-performance
CH:CHROMATOGRAPHY_SUMMARY        	liquid chromatography (UHPLC) system (ELUTE, Bruker). The elution is done at a
CH:CHROMATOGRAPHY_SUMMARY        	flow rate of 300 μL.min-1 with a linear gradient of acetonitrile in 0.1% formic
CH:CHROMATOGRAPHY_SUMMARY        	acid (5 to 90%) over a period of 21 minutes. Next, we analyze the individual
CH:CHROMATOGRAPHY_SUMMARY        	metabolite content using a high-resolution electrospray ionization hybrid
CH:CHROMATOGRAPHY_SUMMARY        	quadrupole time-of-flight (ESI-Qq-TOF) mass spectrometer (Compact, Bruker)
CH:CHROMATOGRAPHY_SUMMARY        	operating in positive auto MS/MS mode.
CH:CHROMATOGRAPHY_TYPE           	Reversed phase
CH:INSTRUMENT_NAME               	ELUTE BRUKER
CH:COLUMN_NAME                   	Thermo Acclaim Polar Advantage II (150 x 3mm,3um)
CH:SOLVENT_A                     	100% water
CH:SOLVENT_B                     	100% acetonitrile; 0.1% formic acid
CH:FLOW_GRADIENT                 	Linear gradient of 5-90% B over 21 min
CH:FLOW_RATE                     	300 uL/min
CH:COLUMN_TEMPERATURE            	40
CH:INTERNAL_STANDARD             	Sodium formate
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
AN:LABORATORY_NAME               	PtSMB
AN:OPERATOR_NAME                 	Benjamin MARIE
#MS
MS:INSTRUMENT_NAME               	Bruker MicrOTOF II
MS:INSTRUMENT_TYPE               	QTOF
MS:MS_TYPE                       	ESI
MS:ION_MODE                      	POSITIVE
MS:MS_COMMENTS                   	For each sample, 2 μL was injected, and molecule separation was performed by a
MS:MS_COMMENTS                   	Polar Advance II 2.5 pore C18 chromatographic column (Thermo Fisher Scientific,
MS:MS_COMMENTS                   	Waltham, MA, USA) at a flow rate of 300 μL.min−1 under a linear gradient of
MS:MS_COMMENTS                   	acetonitrile acidified with 0.1% formic acid (from 5 to 90% in 15 min).
MS:MS_COMMENTS                   	Metabolite contents were analyzed using an electrospray ionization hybrid
MS:MS_COMMENTS                   	quadrupole time-of-flight (ESI-QqTOF) high-resolution mass spectrometer
MS:MS_COMMENTS                   	(Compact, Bruker, Bremen, Germany). The electrospray ionization (ESI) system was
MS:MS_COMMENTS                   	calibrated with a capillary temperature set to 200°C, the source voltage at 3.5
MS:MS_COMMENTS                   	kV, and gas flow rates at 8 L.min-1. Then, ions were analyzed in the range
MS:MS_COMMENTS                   	50–1500 m/z using collision ion dissociation (CID) and autoMS/MS in positive
MS:MS_COMMENTS                   	or negative modes with information dependent acquisition (IDA). Thus, compounds
MS:MS_COMMENTS                   	were analyzed in simple MS positive or negative modes without quadrupole
MS:MS_COMMENTS                   	fragmentation at a 2-Hz frequency acquisition rate, then top-intensity ions (>
MS:MS_COMMENTS                   	5,000 counts in single MS (MS1)) were individually selected by the quadrupole in
MS:MS_COMMENTS                   	a window of 10 Da and fragmented in a collision cell (MS2) with a selective
MS:MS_COMMENTS                   	exclusion window of 30s (except for ions presenting a count-intensity increase
MS:MS_COMMENTS                   	superior to 3 times) with selective ion collision set between according to ion
MS:MS_COMMENTS                   	intensity and mass (10-50 eV ramp; 50/50% time window; 2-8 Hz adapted according
MS:MS_COMMENTS                   	to ion count intensity), in consecutive cycle times of 2.5s. The resulting ions
MS:MS_COMMENTS                   	of the fragmentation of their respective parent were transferred and detected.
MS:MS_RESULTS_FILE               	ST004041_AN006680_Results.txt	UNITS:Peak area (counts)	Has m/z:Neutral masses	Has RT:Yes	RT units:Seconds
#END