#METABOLOMICS WORKBENCH wnsl2820_20250715_183151 DATATRACK_ID:6173 STUDY_ID:ST004066 ANALYSIS_ID:AN006728 PROJECT_ID:PR002553 VERSION 1 CREATED_ON July 22, 2025, 5:24 pm #PROJECT PR:PROJECT_TITLE Development of Novel Pancreatic Cancer Diagnostic Biomarkers via Lipidomic PR:PROJECT_TITLE Profiling PR:PROJECT_SUMMARY Pancreatic ductal adenocarcinoma (PDAC) remains a lethal malignancy due to late PR:PROJECT_SUMMARY diagnosis and the lack of sensitive screening tools. We recently discovered that PR:PROJECT_SUMMARY tumors depend on circulating fatty acids as a major energy source. Accordingly, PR:PROJECT_SUMMARY we conducted a study to identify screening-diagnostic biomarkers for PDAC in the PR:PROJECT_SUMMARY lipid fraction of patient plasma. In this study, we performed non-targeted PR:PROJECT_SUMMARY lipidomics on human and mouse plasma samples and identified eight concordant PR:PROJECT_SUMMARY lipid metabolites. We then established four targeted-lipidomics PR:PROJECT_SUMMARY platforms—acylcarnitines, phospholipids, fatty acid amides, and PR:PROJECT_SUMMARY sphingolipids—and carried out quantitative analysis in two independent patient PR:PROJECT_SUMMARY cohorts. A combined tertiary analysis of both cohorts revealed 20 lipid species PR:PROJECT_SUMMARY (1 acylcarnitine, 1 sphingolipid, and 18 phospholipids) that achieved an AUC ≥ PR:PROJECT_SUMMARY 0.75 for discriminating PDAC patients from healthy controls. A logistic PR:PROJECT_SUMMARY regression model incorporating 11 or more phospholipids yielded an AUC of PR:PROJECT_SUMMARY 0.9207, which further increased to 0.9427 in the validation cohort upon addition PR:PROJECT_SUMMARY of CA19-9. While extensive validation in larger, multicenter cohorts is PR:PROJECT_SUMMARY necessary, these findings highlight the promise of a lipid biomarker panel and PR:PROJECT_SUMMARY multivariate algorithms as novel metabolic indicators for early PDAC diagnosis. PR:INSTITUTE National Cancer Center Graduate School of Cancer Science and Policy PR:LAST_NAME Kang PR:FIRST_NAME Joon Hee PR:ADDRESS 323 Ilsan-ro, Ilsandong-gu, Goyang-si, Gyeonggi-do, 10408, South Korea PR:EMAIL wnsl2820@gmail.com PR:PHONE 82-031-920-2227 #STUDY ST:STUDY_TITLE Development of Novel Pancreatic Cancer Diagnostic Biomarkers via Lipidomic ST:STUDY_TITLE Profiling - Untargeted metabolomics in human plasma ST:STUDY_SUMMARY Pancreatic ductal adenocarcinoma (PDAC) remains a lethal malignancy due to late ST:STUDY_SUMMARY diagnosis and the lack of sensitive screening tools. We recently discovered that ST:STUDY_SUMMARY tumors depend on circulating fatty acids as a major energy source. Accordingly, ST:STUDY_SUMMARY we conducted a study to identify screening-diagnostic biomarkers for PDAC in the ST:STUDY_SUMMARY lipid fraction of patient plasma. In this study, we performed non-targeted ST:STUDY_SUMMARY lipidomics on human and mouse plasma samples and identified eight concordant ST:STUDY_SUMMARY lipid metabolites. We then established four targeted-lipidomics ST:STUDY_SUMMARY platforms—acylcarnitines, phospholipids, fatty acid amides, and ST:STUDY_SUMMARY sphingolipids—and carried out quantitative analysis in two independent patient ST:STUDY_SUMMARY cohorts. A combined tertiary analysis of both cohorts revealed 20 lipid species ST:STUDY_SUMMARY (1 acylcarnitine, 1 sphingolipid, and 18 phospholipids) that achieved an AUC ≥ ST:STUDY_SUMMARY 0.75 for discriminating PDAC patients from healthy controls. A logistic ST:STUDY_SUMMARY regression model incorporating 11 or more phospholipids yielded an AUC of ST:STUDY_SUMMARY 0.9207, which further increased to 0.9427 in the validation cohort upon addition ST:STUDY_SUMMARY of CA19-9. While extensive validation in larger, multicenter cohorts is ST:STUDY_SUMMARY necessary, these findings highlight the promise of a lipid biomarker panel and ST:STUDY_SUMMARY multivariate algorithms as novel metabolic indicators for early PDAC diagnosis. ST:STUDY_SUMMARY The study aims to find plasma metabolite biomarkers for pancreatic cancer using ST:STUDY_SUMMARY human plasma: normal vs pancreatic cancer. ST:INSTITUTE National Cancer Center Graduate School of Cancer Science and Policy ST:LAST_NAME Kang ST:FIRST_NAME Joon Hee ST:ADDRESS 323 Ilsan-ro, Ilsandong-gu, Goyang-si, Gyeonggi-do, 10408, South Korea ST:EMAIL wnsl2820@gmail.com ST:PHONE 82-031-920-2227 #SUBJECT SU:SUBJECT_TYPE Human SU:SUBJECT_SPECIES Homo sapiens SU:TAXONOMY_ID 9606 SU:GENDER Male and female SU:HUMAN_ETHNICITY Easn Asian #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data SUBJECT_SAMPLE_FACTORS - QC_1 Disease:QC | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=QC_1_pos.raw; RAW_FILE_NAME(Raw_file_neg)=QC_1_neg.raw SUBJECT_SAMPLE_FACTORS - QC_2 Disease:QC | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=QC_2_pos.raw; RAW_FILE_NAME(Raw_file_neg)=QC_2_neg.raw SUBJECT_SAMPLE_FACTORS - QC_3 Disease:QC | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=QC_3_pos.raw; RAW_FILE_NAME(Raw_file_neg)=QC_3_neg.raw SUBJECT_SAMPLE_FACTORS - QC_4 Disease:QC | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=QC_4_pos.raw; RAW_FILE_NAME(Raw_file_neg)=QC_4_neg.raw SUBJECT_SAMPLE_FACTORS - QC_5 Disease:QC | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=QC_5_pos.raw; RAW_FILE_NAME(Raw_file_neg)=QC_5_neg.raw SUBJECT_SAMPLE_FACTORS - QC_6 Disease:QC | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=QC_6_pos.raw; RAW_FILE_NAME(Raw_file_neg)=QC_6_neg.raw SUBJECT_SAMPLE_FACTORS - QC_7 Disease:QC | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=QC_7_pos.raw; RAW_FILE_NAME(Raw_file_neg)=QC_7_neg.raw SUBJECT_SAMPLE_FACTORS - QC_8 Disease:QC | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=QC_8_pos.raw; RAW_FILE_NAME(Raw_file_neg)=QC_8_neg.raw SUBJECT_SAMPLE_FACTORS - QC_9 Disease:QC | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=QC_9_pos.raw; RAW_FILE_NAME(Raw_file_neg)=QC_9_neg.raw SUBJECT_SAMPLE_FACTORS - QC_10 Disease:QC | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=QC_10_pos.raw; RAW_FILE_NAME(Raw_file_neg)=QC_10_neg.raw SUBJECT_SAMPLE_FACTORS - QC_11 Disease:QC | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=QC_11_pos.raw; RAW_FILE_NAME(Raw_file_neg)=QC_11_neg.raw SUBJECT_SAMPLE_FACTORS - QC_12 Disease:QC | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=QC_12_pos.raw; RAW_FILE_NAME(Raw_file_neg)=QC_12_neg.raw SUBJECT_SAMPLE_FACTORS - QC_13 Disease:QC | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=QC_13_pos.raw; RAW_FILE_NAME(Raw_file_neg)=QC_13_neg.raw SUBJECT_SAMPLE_FACTORS - QC_14 Disease:QC | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=QC_14_pos.raw; RAW_FILE_NAME(Raw_file_neg)=QC_14_neg.raw SUBJECT_SAMPLE_FACTORS - QC_15 Disease:QC | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=QC_15_pos.raw; RAW_FILE_NAME(Raw_file_neg)=QC_15_neg.raw SUBJECT_SAMPLE_FACTORS - QC_16 Disease:QC | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=QC_16_pos.raw; RAW_FILE_NAME(Raw_file_neg)=QC_16_neg.raw SUBJECT_SAMPLE_FACTORS - QC_17 Disease:QC | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=QC_17_pos.raw; RAW_FILE_NAME(Raw_file_neg)=QC_17_neg.raw SUBJECT_SAMPLE_FACTORS - QC_18 Disease:QC | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=QC_18_pos.raw; RAW_FILE_NAME(Raw_file_neg)=QC_18_neg.raw SUBJECT_SAMPLE_FACTORS - QC_19 Disease:QC | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=QC_19_pos.raw; RAW_FILE_NAME(Raw_file_neg)=QC_19_neg.raw SUBJECT_SAMPLE_FACTORS - QC_20 Disease:QC | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=QC_20_pos.raw; RAW_FILE_NAME(Raw_file_neg)=QC_20_neg.raw SUBJECT_SAMPLE_FACTORS - Control_1 Disease:Normal | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Control_1_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Control_1_neg.raw SUBJECT_SAMPLE_FACTORS - Control_2 Disease:Normal | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Control_2_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Control_2_neg.raw SUBJECT_SAMPLE_FACTORS - Control_3 Disease:Normal | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Control_3_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Control_3_neg.raw SUBJECT_SAMPLE_FACTORS - Control_4 Disease:Normal | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Control_4_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Control_4_neg.raw SUBJECT_SAMPLE_FACTORS - Control_5 Disease:Normal | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Control_5_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Control_5_neg.raw SUBJECT_SAMPLE_FACTORS - Control_6 Disease:Normal | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Control_6_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Control_6_neg.raw SUBJECT_SAMPLE_FACTORS - Control_7 Disease:Normal | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Control_7_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Control_7_neg.raw SUBJECT_SAMPLE_FACTORS - Control_8 Disease:Normal | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Control_8_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Control_8_neg.raw SUBJECT_SAMPLE_FACTORS - Control_9 Disease:Normal | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Control_9_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Control_9_neg.raw SUBJECT_SAMPLE_FACTORS - Control_10 Disease:Normal | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Control_10_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Control_10_neg.raw SUBJECT_SAMPLE_FACTORS - Control_11 Disease:Normal | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Control_11_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Control_11_neg.raw SUBJECT_SAMPLE_FACTORS - Control_12 Disease:Normal | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Control_12_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Control_12_neg.raw SUBJECT_SAMPLE_FACTORS - Control_13 Disease:Normal | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Control_13_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Control_13_neg.raw SUBJECT_SAMPLE_FACTORS - Control_14 Disease:Normal | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Control_14_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Control_14_neg.raw SUBJECT_SAMPLE_FACTORS - Control_15 Disease:Normal | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Control_15_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Control_15_neg.raw SUBJECT_SAMPLE_FACTORS - Control_16 Disease:Normal | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Control_16_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Control_16_neg.raw SUBJECT_SAMPLE_FACTORS - Control_17 Disease:Normal | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Control_17_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Control_17_neg.raw SUBJECT_SAMPLE_FACTORS - Control_18 Disease:Normal | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Control_18_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Control_18_neg.raw SUBJECT_SAMPLE_FACTORS - Control_19 Disease:Normal | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Control_19_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Control_19_neg.raw SUBJECT_SAMPLE_FACTORS - Control_20 Disease:Normal | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Control_20_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Control_20_neg.raw SUBJECT_SAMPLE_FACTORS - Control_21 Disease:Normal | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Control_21_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Control_21_neg.raw SUBJECT_SAMPLE_FACTORS - Control_22 Disease:Normal | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Control_22_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Control_22_neg.raw SUBJECT_SAMPLE_FACTORS - Control_23 Disease:Normal | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Control_23_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Control_23_neg.raw SUBJECT_SAMPLE_FACTORS - Control_24 Disease:Normal | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Control_24_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Control_24_neg.raw SUBJECT_SAMPLE_FACTORS - Control_25 Disease:Normal | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Control_25_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Control_25_neg.raw SUBJECT_SAMPLE_FACTORS - Control_26 Disease:Normal | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Control_26_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Control_26_neg.raw SUBJECT_SAMPLE_FACTORS - Control_27 Disease:Normal | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Control_27_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Control_27_neg.raw SUBJECT_SAMPLE_FACTORS - Control_28 Disease:Normal | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Control_28_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Control_28_neg.raw SUBJECT_SAMPLE_FACTORS - Control_29 Disease:Normal | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Control_29_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Control_29_neg.raw SUBJECT_SAMPLE_FACTORS - Control_30 Disease:Normal | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Control_30_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Control_30_neg.raw SUBJECT_SAMPLE_FACTORS - Control_32 Disease:Normal | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Control_32_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Control_32_neg.raw SUBJECT_SAMPLE_FACTORS - Control_33 Disease:Normal | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Control_33_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Control_33_neg.raw SUBJECT_SAMPLE_FACTORS - Control_34 Disease:Normal | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Control_34_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Control_34_neg.raw SUBJECT_SAMPLE_FACTORS - Control_35 Disease:Normal | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Control_35_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Control_35_neg.raw SUBJECT_SAMPLE_FACTORS - Control_36 Disease:Normal | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Control_36_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Control_36_neg.raw SUBJECT_SAMPLE_FACTORS - Control_37 Disease:Normal | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Control_37_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Control_37_neg.raw SUBJECT_SAMPLE_FACTORS - Control_38 Disease:Normal | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Control_38_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Control_38_neg.raw SUBJECT_SAMPLE_FACTORS - Control_39 Disease:Normal | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Control_39_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Control_39_neg.raw SUBJECT_SAMPLE_FACTORS - Control_40 Disease:Normal | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Control_40_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Control_40_neg.raw SUBJECT_SAMPLE_FACTORS - Control_41 Disease:Normal | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Control_41_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Control_41_neg.raw SUBJECT_SAMPLE_FACTORS - Control_42 Disease:Normal | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Control_42_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Control_42_neg.raw SUBJECT_SAMPLE_FACTORS - Control_43 Disease:Normal | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Control_43_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Control_43_neg.raw SUBJECT_SAMPLE_FACTORS - Control_44 Disease:Normal | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Control_44_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Control_44_neg.raw SUBJECT_SAMPLE_FACTORS - Control_45 Disease:Normal | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Control_45_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Control_45_neg.raw SUBJECT_SAMPLE_FACTORS - Control_46 Disease:Normal | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Control_46_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Control_46_neg.raw SUBJECT_SAMPLE_FACTORS - Control_47 Disease:Normal | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Control_47_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Control_47_neg.raw SUBJECT_SAMPLE_FACTORS - Control_48 Disease:Normal | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Control_48_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Control_48_neg.raw SUBJECT_SAMPLE_FACTORS - Control_49 Disease:Normal | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Control_49_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Control_49_neg.raw SUBJECT_SAMPLE_FACTORS - Control_50 Disease:Normal | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Control_50_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Control_50_neg.raw SUBJECT_SAMPLE_FACTORS - Control_51 Disease:Normal | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Control_51_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Control_51_neg.raw SUBJECT_SAMPLE_FACTORS - Control_52 Disease:Normal | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Control_52_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Control_52_neg.raw SUBJECT_SAMPLE_FACTORS - Control_53 Disease:Normal | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Control_53_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Control_53_neg.raw SUBJECT_SAMPLE_FACTORS - Control_54 Disease:Normal | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Control_54_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Control_54_neg.raw SUBJECT_SAMPLE_FACTORS - Control_55 Disease:Normal | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Control_55_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Control_55_neg.raw SUBJECT_SAMPLE_FACTORS - Control_56 Disease:Normal | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Control_56_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Control_56_neg.raw SUBJECT_SAMPLE_FACTORS - Control_58 Disease:Normal | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Control_58_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Control_58_neg.raw SUBJECT_SAMPLE_FACTORS - Control_59 Disease:Normal | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Control_59_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Control_59_neg.raw SUBJECT_SAMPLE_FACTORS - Control_60 Disease:Normal | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Control_60_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Control_60_neg.raw SUBJECT_SAMPLE_FACTORS - Control_62 Disease:Normal | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Control_62_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Control_62_neg.raw SUBJECT_SAMPLE_FACTORS - Control_63 Disease:Normal | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Control_63_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Control_63_neg.raw SUBJECT_SAMPLE_FACTORS - Control_64 Disease:Normal | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Control_64_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Control_64_neg.raw SUBJECT_SAMPLE_FACTORS - Control_66 Disease:Normal | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Control_66_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Control_66_neg.raw SUBJECT_SAMPLE_FACTORS - Control_67 Disease:Normal | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Control_67_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Control_67_neg.raw SUBJECT_SAMPLE_FACTORS - Control_69 Disease:Normal | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Control_69_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Control_69_neg.raw SUBJECT_SAMPLE_FACTORS - Control_70 Disease:Normal | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Control_70_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Control_70_neg.raw SUBJECT_SAMPLE_FACTORS - Control_71 Disease:Normal | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Control_71_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Control_71_neg.raw SUBJECT_SAMPLE_FACTORS - Control_72 Disease:Normal | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Control_72_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Control_72_neg.raw SUBJECT_SAMPLE_FACTORS - Control_73 Disease:Normal | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Control_73_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Control_73_neg.raw SUBJECT_SAMPLE_FACTORS - Control_74 Disease:Normal | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Control_74_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Control_74_neg.raw SUBJECT_SAMPLE_FACTORS - Control_75 Disease:Normal | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Control_75_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Control_75_neg.raw SUBJECT_SAMPLE_FACTORS - Control_76 Disease:Normal | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Control_76_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Control_76_neg.raw SUBJECT_SAMPLE_FACTORS - Control_77 Disease:Normal | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Control_77_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Control_77_neg.raw SUBJECT_SAMPLE_FACTORS - Control_78 Disease:Normal | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Control_78_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Control_78_neg.raw SUBJECT_SAMPLE_FACTORS - Control_79 Disease:Normal | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Control_79_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Control_79_neg.raw SUBJECT_SAMPLE_FACTORS - Control_80 Disease:Normal | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Control_80_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Control_80_neg.raw SUBJECT_SAMPLE_FACTORS - Control_81 Disease:Normal | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Control_81_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Control_81_neg.raw SUBJECT_SAMPLE_FACTORS - Control_82 Disease:Normal | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Control_82_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Control_82_neg.raw SUBJECT_SAMPLE_FACTORS - Control_83 Disease:Normal | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Control_83_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Control_83_neg.raw SUBJECT_SAMPLE_FACTORS - Control_84 Disease:Normal | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Control_84_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Control_84_neg.raw SUBJECT_SAMPLE_FACTORS - Control_85 Disease:Normal | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Control_85_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Control_85_neg.raw SUBJECT_SAMPLE_FACTORS - Control_86 Disease:Normal | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Control_86_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Control_86_neg.raw SUBJECT_SAMPLE_FACTORS - Control_87 Disease:Normal | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Control_87_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Control_87_neg.raw SUBJECT_SAMPLE_FACTORS - Control_88 Disease:Normal | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Control_88_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Control_88_neg.raw SUBJECT_SAMPLE_FACTORS - Control_89 Disease:Normal | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Control_89_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Control_89_neg.raw SUBJECT_SAMPLE_FACTORS - Control_90 Disease:Normal | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Control_90_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Control_90_neg.raw SUBJECT_SAMPLE_FACTORS - Control_91 Disease:Normal | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Control_91_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Control_91_neg.raw SUBJECT_SAMPLE_FACTORS - Control_92 Disease:Normal | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Control_92_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Control_92_neg.raw SUBJECT_SAMPLE_FACTORS - Control_93 Disease:Normal | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Control_93_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Control_93_neg.raw SUBJECT_SAMPLE_FACTORS - Control_94 Disease:Normal | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Control_94_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Control_94_neg.raw SUBJECT_SAMPLE_FACTORS - Control_97 Disease:Normal | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Control_97_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Control_97_neg.raw SUBJECT_SAMPLE_FACTORS - Control_98 Disease:Normal | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Control_98_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Control_98_neg.raw SUBJECT_SAMPLE_FACTORS - Control_99 Disease:Normal | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Control_99_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Control_99_neg.raw SUBJECT_SAMPLE_FACTORS - Control_100 Disease:Normal | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Control_100_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Control_100_neg.raw SUBJECT_SAMPLE_FACTORS - Control_101 Disease:Normal | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Control_101_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Control_101_neg.raw SUBJECT_SAMPLE_FACTORS - Control_102 Disease:Normal | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Control_102_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Control_102_neg.raw SUBJECT_SAMPLE_FACTORS - Control_103 Disease:Normal | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Control_103_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Control_103_neg.raw SUBJECT_SAMPLE_FACTORS - Control_104 Disease:Normal | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Control_104_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Control_104_neg.raw SUBJECT_SAMPLE_FACTORS - Control_105 Disease:Normal | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Control_105_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Control_105_neg.raw SUBJECT_SAMPLE_FACTORS - Control_106 Disease:Normal | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Control_106_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Control_106_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_1 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_1_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_1_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_2 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_2_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_2_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_3 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_3_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_3_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_4 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_4_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_4_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_5 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_5_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_5_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_6 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_6_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_6_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_7 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_7_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_7_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_8 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_8_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_8_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_9 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_9_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_9_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_10 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_10_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_10_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_11 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_11_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_11_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_12 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_12_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_12_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_13 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_13_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_13_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_14 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_14_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_14_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_15 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_15_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_15_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_16 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_16_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_16_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_17 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_17_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_17_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_18 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_18_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_18_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_19 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_19_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_19_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_20 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_20_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_20_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_21 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_21_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_21_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_22 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_22_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_22_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_23 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_23_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_23_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_24 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_24_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_24_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_25 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_25_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_25_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_26 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_26_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_26_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_27 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_27_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_27_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_28 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_28_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_28_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_29 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_29_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_29_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_30 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_30_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_30_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_31 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_31_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_31_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_32 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_32_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_32_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_33 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_33_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_33_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_34 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_34_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_34_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_35 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_35_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_35_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_36 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_36_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_36_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_37 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_37_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_37_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_38 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_38_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_38_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_39 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_39_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_39_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_40 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_40_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_40_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_41 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_41_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_41_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_42 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_42_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_42_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_43 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_43_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_43_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_44 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_44_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_44_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_45 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_45_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_45_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_46 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_46_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_46_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_47 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_47_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_47_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_48 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_48_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_48_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_49 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_49_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_49_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_50 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_50_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_50_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_51 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_51_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_51_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_52 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_52_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_52_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_53 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_53_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_53_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_54 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_54_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_54_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_55 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_55_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_55_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_56 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_56_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_56_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_57 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_57_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_57_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_58 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_58_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_58_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_59 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_59_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_59_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_60 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_60_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_60_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_61 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_61_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_61_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_62 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_62_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_62_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_63 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_63_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_63_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_64 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_64_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_64_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_65 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_65_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_65_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_66 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_66_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_66_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_67 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_67_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_67_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_68 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_68_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_68_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_69 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_69_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_69_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_70 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_70_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_70_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_71 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_71_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_71_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_72 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_72_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_72_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_73 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_73_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_73_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_74 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_74_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_74_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_75 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_75_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_75_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_76 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_76_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_76_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_77 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_77_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_77_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_78 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_78_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_78_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_79 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_79_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_79_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_80 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_80_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_80_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_81 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_81_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_81_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_82 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_82_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_82_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_83 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_83_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_83_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_84 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_84_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_84_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_85 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_85_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_85_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_86 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_86_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_86_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_87 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_87_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_87_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_88 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_88_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_88_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_89 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_89_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_89_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_90 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_90_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_90_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_91 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_91_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_91_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_92 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_92_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_92_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_93 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_93_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_93_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_94 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_94_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_94_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_95 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_95_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_95_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_96 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_96_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_96_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_97 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_97_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_97_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_98 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_98_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_98_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_99 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_99_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_99_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_100 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_100_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_100_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_101 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_101_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_101_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_102 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_102_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_102_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_103 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_103_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_103_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_104 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_104_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_104_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_105 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_105_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_105_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_106 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_106_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_106_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_107 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_107_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_107_neg.raw SUBJECT_SAMPLE_FACTORS - Disease_108 Disease:Pancreatic_cancer | Sample source:human_plasma RAW_FILE_NAME(Raw_file_pos)=Disease_108_pos.raw; RAW_FILE_NAME(Raw_file_neg)=Disease_108_neg.raw #COLLECTION CO:COLLECTION_SUMMARY Human plasma was collected using EDTA tubes. CO:SAMPLE_TYPE Blood (plasma) CO:COLLECTION_LOCATION vein CO:COLLECTION_FREQUENCY 1 CO:COLLECTION_DURATION 2017-2023 CO:VOLUMEORAMOUNT_COLLECTED 2ml CO:STORAGE_CONDITIONS -80℃ CO:COLLECTION_VIALS EDTA tube CO:STORAGE_VIALS 1.8ml micro-tube CO:COLLECTION_TUBE_TEMP RT #TREATMENT TR:TREATMENT_SUMMARY Human plasma was stored at deep freezer until analysis.Blood samples for the TR:TREATMENT_SUMMARY control group were obtained from subjects undergoing routine health examinations TR:TREATMENT_SUMMARY at our institution, while samples for the pancreatic cancer group were those TR:TREATMENT_SUMMARY collected for preoperative blood testing. No pharmacological treatments were TR:TREATMENT_SUMMARY administered to the pancreatic cancer patients. TR:TREATMENT none TR:TREATMENT_COMPOUND none TR:TREATMENT_ROUTE none TR:TREATMENT_DOSE none TR:TREATMENT_DOSEVOLUME none TR:TREATMENT_DOSEDURATION none TR:TREATMENT_VEHICLE none TR:HUMAN_FASTING none TR:HUMAN_ENDP_CLINICAL_SIGNS none #SAMPLEPREP SP:SAMPLEPREP_SUMMARY Metabolites were extracted from human (or mouse) plasma using conventional SP:SAMPLEPREP_SUMMARY liquid-liquid extraction procedures. {Bligh,E.G. and Dyer,W.J. SP:SAMPLEPREP_SUMMARY Can.J.Biochem.Physiol. 1959,37:911}. Briefly, 3-4 volumes of chloroform/methanol SP:SAMPLEPREP_SUMMARY (1/2, v/v) were added to 50 μL of human (or mouse) plasma, and then centrifuged SP:SAMPLEPREP_SUMMARY for 15 min. Additional 100 uL of chloroform or water was added if phase SP:SAMPLEPREP_SUMMARY separation could not be achieved. Nonpolar metabolites containing lipids were SP:SAMPLEPREP_SUMMARY collected from the lower organic phase. The organic solution was dried using SP:SAMPLEPREP_SUMMARY vacuum centrifuge, then stored at -20oC until analysis. The dried matter of SP:SAMPLEPREP_SUMMARY organic with 100 μL of methanol, respectively, prior to LC-MS analysis. All SP:SAMPLEPREP_SUMMARY solvents were purchased from Sigma-Aldrich (St. Louis, MO) or Thermo Fischer SP:SAMPLEPREP_SUMMARY Scientific (Waltham, MA). SP:PROCESSING_STORAGE_CONDITIONS 4℃ SP:EXTRACTION_METHOD liquid liquid extraction SP:EXTRACT_ENRICHMENT Resuspension after vaccum dry SP:EXTRACT_CLEANUP none SP:EXTRACT_STORAGE -20℃ SP:SAMPLE_RESUSPENSION 50uL methanol SP:SAMPLE_DERIVATIZATION none SP:SAMPLE_SPIKING none #CHROMATOGRAPHY CH:CHROMATOGRAPHY_SUMMARY Column - Part Number: Agilent A3000150X021 CH:CHROMATOGRAPHY_TYPE Reversed phase CH:INSTRUMENT_NAME Thermo Dionex Ultimate 3000 CH:COLUMN_NAME Agilent Pursuit 5 C18 (150 x 2.1mm, 5um) CH:SOLVENT_A 100% water; 0.1 % formic acid CH:SOLVENT_B 85% methanol/15% isopropanol; 0.1 % formic acid CH:FLOW_GRADIENT 75 to 99.9 % of B for 2 min, hold at 99.9 % of B for 13 min, 99.9 to 75 % of B CH:FLOW_GRADIENT for 0.5 min, and then hold at 75 % of B for 4.5 min CH:FLOW_RATE 300 ul/min CH:COLUMN_TEMPERATURE 40 #ANALYSIS AN:ANALYSIS_TYPE MS #MS MS:INSTRUMENT_NAME Thermo LTQ XL MS:INSTRUMENT_TYPE Single quadrupole MS:MS_TYPE ESI MS:ION_MODE POSITIVE MS:MS_COMMENTS Metabolite features, including neutral mass and retention time values, were MS:MS_COMMENTS determined using Compound Discoverer 3.3. HMDB, KEGG, and LipidMaps were used MS:MS_COMMENTS for DB searches with 10 ppm mass accuracy. mzCloud, LipidBlasts, and in-house DB MS:MS_COMMENTS were used for mass spectral MS/MS searches MS:MS_RESULTS_FILE ST004066_AN006728_Results.txt UNITS:Peak area Has m/z:Yes Has RT:Yes RT units:Minutes #END