#METABOLOMICS WORKBENCH mjackstadt1_20250701_114942 DATATRACK_ID:6122 STUDY_ID:ST004075 ANALYSIS_ID:AN006745 PROJECT_ID:PR002557
VERSION             	1
CREATED_ON             	July 24, 2025, 11:45 am
#PROJECT
PR:PROJECT_TITLE                 	Glucose-derived sorbitol drives steatotic liver disease in the absence of gut
PR:PROJECT_TITLE                 	bacteria
PR:PROJECT_SUMMARY               	The role of the gut microbiome in mediating steatotic liver disease is poorly
PR:PROJECT_SUMMARY               	understood. Here, we developed a model to deplete the gut microbiome in adult
PR:PROJECT_SUMMARY               	zebrafish and discovered that it led to steatotic liver disease in animals on
PR:PROJECT_SUMMARY               	standard diets. Using metabolomics and isotope tracing, we found that dietary
PR:PROJECT_SUMMARY               	glucose is transformed into sorbitol within the intestine. While bacteria
PR:PROJECT_SUMMARY               	degrade the sorbitol in control animals, sorbitol is passed to the livers of
PR:PROJECT_SUMMARY               	fish in which the gut microbiome has been depleted. Within the liver, sorbitol
PR:PROJECT_SUMMARY               	is converted into fructose 1-phosphate, which subsequently activates
PR:PROJECT_SUMMARY               	glucokinase. The result is increased hepatic metabolism of glucose, leading to
PR:PROJECT_SUMMARY               	elevated glycogen and fat content. Inhibition of sorbitol production in
PR:PROJECT_SUMMARY               	microbiome-depleted animals was sufficient to prevent the development of
PR:PROJECT_SUMMARY               	steatotic liver, and colonizing sorbitol-degrading bacteria in the intestines of
PR:PROJECT_SUMMARY               	microbiome-depleted fish reversed the steatotic liver phenotype. Taken together,
PR:PROJECT_SUMMARY               	these findings show that sorbitol-degrading bacteria in the gut protect against
PR:PROJECT_SUMMARY               	steatotic liver disease.
PR:INSTITUTE                     	Washington University in St. Louis
PR:LAST_NAME                     	Jackstadt
PR:FIRST_NAME                    	Madelyn
PR:ADDRESS                       	One Brookings Drive, St. Louis, Missouri, 63130, USA
PR:EMAIL                         	mjackstadt@wustl.edu
PR:PHONE                         	3149356405
#STUDY
ST:STUDY_TITLE                   	[U-13C]Glucose Labeling in Adult Zebrafish Treated with Antimicrobials or
ST:STUDY_TITLE                   	Control
ST:STUDY_SUMMARY                 	Glucose metabolism was traced with [U-13C]glucose in the intestine, liver, and
ST:STUDY_SUMMARY                 	serum of adult zebrafish with in-tact or depleted gut microbiota. We found that
ST:STUDY_SUMMARY                 	fish treated with antimicrobials had elevated M+6 sorbitol relative to control
ST:STUDY_SUMMARY                 	in these organs.
ST:INSTITUTE                     	Washington University in St. Louis
ST:LAST_NAME                     	Jackstadt
ST:FIRST_NAME                    	Madelyn
ST:ADDRESS                       	One Brookings Drive, St. Louis, Missouri, 63130, USA
ST:EMAIL                         	mjackstadt@wustl.edu
ST:PHONE                         	3149356405
#SUBJECT
SU:SUBJECT_TYPE                  	Fish
SU:SUBJECT_SPECIES               	Danio rerio
SU:TAXONOMY_ID                   	7955
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data
SUBJECT_SAMPLE_FACTORS           	-	UGlucose_Neg_Ctrl_16_Serum	Treatment:Control | Sample source:Serum	RAW_FILE_NAME(Raw File Name)=UGlucose_Neg_Ctrl_16_Serum.mzML
SUBJECT_SAMPLE_FACTORS           	-	UGlucose_Neg_Ctrl_17_Serum	Treatment:Control | Sample source:Serum	RAW_FILE_NAME(Raw File Name)=UGlucose_Neg_Ctrl_17_Serum.mzML
SUBJECT_SAMPLE_FACTORS           	-	UGlucose_Neg_Ctrl_18_Serum	Treatment:Control | Sample source:Serum	RAW_FILE_NAME(Raw File Name)=UGlucose_Neg_Ctrl_18_Serum.mzML
SUBJECT_SAMPLE_FACTORS           	-	UGlucose_Neg_Ctrl_19_Serum	Treatment:Control | Sample source:Serum	RAW_FILE_NAME(Raw File Name)=UGlucose_Neg_Ctrl_19_Serum.mzML
SUBJECT_SAMPLE_FACTORS           	-	UGlucose_Neg_Ctrl_20_Serum	Treatment:Control | Sample source:Serum	RAW_FILE_NAME(Raw File Name)=UGlucose_Neg_Ctrl_20_Serum.mzML
SUBJECT_SAMPLE_FACTORS           	-	UGlucose_Neg_Ctrl_21_Serum	Treatment:Control | Sample source:Serum	RAW_FILE_NAME(Raw File Name)=UGlucose_Neg_Ctrl_21_Serum.mzML
SUBJECT_SAMPLE_FACTORS           	-	UGlucose_Neg_Ctrl_22_Serum	Treatment:Control | Sample source:Serum	RAW_FILE_NAME(Raw File Name)=UGlucose_Neg_Ctrl_22_Serum.mzML
SUBJECT_SAMPLE_FACTORS           	-	UGlucose_Neg_Ctrl_23_Serum	Treatment:Control | Sample source:Serum	RAW_FILE_NAME(Raw File Name)=UGlucose_Neg_Ctrl_23_Serum.mzML
SUBJECT_SAMPLE_FACTORS           	-	UGlucose_Neg_Anti_24_Serum	Treatment:Antimicrobials | Sample source:Serum	RAW_FILE_NAME(Raw File Name)=UGlucose_Neg_Anti_24_Serum.mzML
SUBJECT_SAMPLE_FACTORS           	-	UGlucose_Neg_Anti_26_Serum	Treatment:Antimicrobials | Sample source:Serum	RAW_FILE_NAME(Raw File Name)=UGlucose_Neg_Anti_26_Serum.mzML
SUBJECT_SAMPLE_FACTORS           	-	UGlucose_Neg_Anti_27_Serum	Treatment:Antimicrobials | Sample source:Serum	RAW_FILE_NAME(Raw File Name)=UGlucose_Neg_Anti_27_Serum.mzML
SUBJECT_SAMPLE_FACTORS           	-	UGlucose_Neg_Anti_28_Serum	Treatment:Antimicrobials | Sample source:Serum	RAW_FILE_NAME(Raw File Name)=UGlucose_Neg_Anti_28_Serum.mzML
SUBJECT_SAMPLE_FACTORS           	-	UGlucose_Neg_Anti_29_Serum	Treatment:Antimicrobials | Sample source:Serum	RAW_FILE_NAME(Raw File Name)=UGlucose_Neg_Anti_29_Serum.mzML
SUBJECT_SAMPLE_FACTORS           	-	UGlucose_Neg_Anti_30_Serum	Treatment:Antimicrobials | Sample source:Serum	RAW_FILE_NAME(Raw File Name)=UGlucose_Neg_Anti_30_Serum.mzML
SUBJECT_SAMPLE_FACTORS           	-	UGlucose_Neg_Anti_31_Serum	Treatment:Antimicrobials | Sample source:Serum	RAW_FILE_NAME(Raw File Name)=UGlucose_Neg_Anti_31_Serum.mzML
SUBJECT_SAMPLE_FACTORS           	-	UGlucose_Neg_Ctrl_16_Liver	Treatment:Control | Sample source:Liver	RAW_FILE_NAME(Raw File Name)=UGlucose_Neg_Ctrl_16_Liver.mzML
SUBJECT_SAMPLE_FACTORS           	-	UGlucose_Neg_Ctrl_17_Liver	Treatment:Control | Sample source:Liver	RAW_FILE_NAME(Raw File Name)=UGlucose_Neg_Ctrl_17_Liver.mzML
SUBJECT_SAMPLE_FACTORS           	-	UGlucose_Neg_Ctrl_18_Liver	Treatment:Control | Sample source:Liver	RAW_FILE_NAME(Raw File Name)=UGlucose_Neg_Ctrl_18_Liver.mzML
SUBJECT_SAMPLE_FACTORS           	-	UGlucose_Neg_Ctrl_19_Liver	Treatment:Control | Sample source:Liver	RAW_FILE_NAME(Raw File Name)=UGlucose_Neg_Ctrl_19_Liver.mzML
SUBJECT_SAMPLE_FACTORS           	-	UGlucose_Neg_Ctrl_20_Liver	Treatment:Control | Sample source:Liver	RAW_FILE_NAME(Raw File Name)=UGlucose_Neg_Ctrl_20_Liver.mzML
SUBJECT_SAMPLE_FACTORS           	-	UGlucose_Neg_Ctrl_21_Liver	Treatment:Control | Sample source:Liver	RAW_FILE_NAME(Raw File Name)=UGlucose_Neg_Ctrl_21_Liver.mzML
SUBJECT_SAMPLE_FACTORS           	-	UGlucose_Neg_Ctrl_23_Liver	Treatment:Control | Sample source:Liver	RAW_FILE_NAME(Raw File Name)=UGlucose_Neg_Ctrl_23_Liver.mzML
SUBJECT_SAMPLE_FACTORS           	-	UGlucose_Neg_Anti_24_Liver	Treatment:Antimicrobials | Sample source:Liver	RAW_FILE_NAME(Raw File Name)=UGlucose_Neg_Anti_24_Liver.mzML
SUBJECT_SAMPLE_FACTORS           	-	UGlucose_Neg_Anti_26_Liver	Treatment:Antimicrobials | Sample source:Liver	RAW_FILE_NAME(Raw File Name)=UGlucose_Neg_Anti_26_Liver.mzML
SUBJECT_SAMPLE_FACTORS           	-	UGlucose_Neg_Anti_27_Liver	Treatment:Antimicrobials | Sample source:Liver	RAW_FILE_NAME(Raw File Name)=UGlucose_Neg_Anti_27_Liver.mzML
SUBJECT_SAMPLE_FACTORS           	-	UGlucose_Neg_Anti_28_Liver	Treatment:Antimicrobials | Sample source:Liver	RAW_FILE_NAME(Raw File Name)=UGlucose_Neg_Anti_28_Liver.mzML
SUBJECT_SAMPLE_FACTORS           	-	UGlucose_Neg_Anti_29_Liver	Treatment:Antimicrobials | Sample source:Liver	RAW_FILE_NAME(Raw File Name)=UGlucose_Neg_Anti_29_Liver.mzML
SUBJECT_SAMPLE_FACTORS           	-	UGlucose_Neg_Anti_30_Liver	Treatment:Antimicrobials | Sample source:Liver	RAW_FILE_NAME(Raw File Name)=UGlucose_Neg_Anti_30_Liver.mzML
SUBJECT_SAMPLE_FACTORS           	-	UGlucose_Neg_Anti_31_Liver	Treatment:Antimicrobials | Sample source:Liver	RAW_FILE_NAME(Raw File Name)=UGlucose_Neg_Anti_31_Liver.mzML
SUBJECT_SAMPLE_FACTORS           	-	UGlucose_Neg_Ctrl_16_Intestine	Treatment:Control | Sample source:Intestine	RAW_FILE_NAME(Raw File Name)=UGlucose_Neg_Ctrl_16_Intestine.mzML
SUBJECT_SAMPLE_FACTORS           	-	UGlucose_Neg_Ctrl_17_Intestine	Treatment:Control | Sample source:Intestine	RAW_FILE_NAME(Raw File Name)=UGlucose_Neg_Ctrl_17_Intestine.mzML
SUBJECT_SAMPLE_FACTORS           	-	UGlucose_Neg_Ctrl_18_Intestine	Treatment:Control | Sample source:Intestine	RAW_FILE_NAME(Raw File Name)=UGlucose_Neg_Ctrl_18_Intestine.mzML
SUBJECT_SAMPLE_FACTORS           	-	UGlucose_Neg_Ctrl_19_Intestine	Treatment:Control | Sample source:Intestine	RAW_FILE_NAME(Raw File Name)=UGlucose_Neg_Ctrl_19_Intestine.mzML
SUBJECT_SAMPLE_FACTORS           	-	UGlucose_Neg_Ctrl_20_Intestine	Treatment:Control | Sample source:Intestine	RAW_FILE_NAME(Raw File Name)=UGlucose_Neg_Ctrl_20_Intestine.mzML
SUBJECT_SAMPLE_FACTORS           	-	UGlucose_Neg_Ctrl_21_Intestine	Treatment:Control | Sample source:Intestine	RAW_FILE_NAME(Raw File Name)=UGlucose_Neg_Ctrl_21_Intestine.mzML
SUBJECT_SAMPLE_FACTORS           	-	UGlucose_Neg_Ctrl_22_Intestine	Treatment:Control | Sample source:Intestine	RAW_FILE_NAME(Raw File Name)=UGlucose_Neg_Ctrl_22_Intestine.mzML
SUBJECT_SAMPLE_FACTORS           	-	UGlucose_Neg_Ctrl_23_Intestine	Treatment:Control | Sample source:Intestine	RAW_FILE_NAME(Raw File Name)=UGlucose_Neg_Ctrl_23_Intestine.mzML
SUBJECT_SAMPLE_FACTORS           	-	UGlucose_Neg_Anti_24_Intestine	Treatment:Antimicrobials | Sample source:Intestine	RAW_FILE_NAME(Raw File Name)=UGlucose_Neg_Anti_24_Intestine.mzML
SUBJECT_SAMPLE_FACTORS           	-	UGlucose_Neg_Anti_26_Intestine	Treatment:Antimicrobials | Sample source:Intestine	RAW_FILE_NAME(Raw File Name)=UGlucose_Neg_Anti_26_Intestine.mzML
SUBJECT_SAMPLE_FACTORS           	-	UGlucose_Neg_Anti_27_Intestine	Treatment:Antimicrobials | Sample source:Intestine	RAW_FILE_NAME(Raw File Name)=UGlucose_Neg_Anti_27_Intestine.mzML
SUBJECT_SAMPLE_FACTORS           	-	UGlucose_Neg_Anti_28_Intestine	Treatment:Antimicrobials | Sample source:Intestine	RAW_FILE_NAME(Raw File Name)=UGlucose_Neg_Anti_28_Intestine.mzML
SUBJECT_SAMPLE_FACTORS           	-	UGlucose_Neg_Anti_29_Intestine	Treatment:Antimicrobials | Sample source:Intestine	RAW_FILE_NAME(Raw File Name)=UGlucose_Neg_Anti_29_Intestine.mzML
SUBJECT_SAMPLE_FACTORS           	-	UGlucose_Neg_Anti_30_Intestine	Treatment:Antimicrobials | Sample source:Intestine	RAW_FILE_NAME(Raw File Name)=UGlucose_Neg_Anti_30_Intestine.mzML
SUBJECT_SAMPLE_FACTORS           	-	UGlucose_Neg_Anti_31_Intestine	Treatment:Antimicrobials | Sample source:Intestine	RAW_FILE_NAME(Raw File Name)=UGlucose_Neg_Anti_31_Intestine.mzML
#COLLECTION
CO:COLLECTION_SUMMARY            	Zebrafish were anesthetized and thoroughly dried before a scalpel was used to
CO:COLLECTION_SUMMARY            	excise the caudal fin and some attached muscle tissue. Zebrafish were then
CO:COLLECTION_SUMMARY            	immediately placed in a spin column such that blood from the wound could flow
CO:COLLECTION_SUMMARY            	from the fish (in a 1.5 mL microcentrifuge tube with a small hole in the bottom)
CO:COLLECTION_SUMMARY            	to a collection tube (a 0.5 mL microcentrifuge tube) during a brief, low-speed
CO:COLLECTION_SUMMARY            	centrifugation (40 g for 1 min at 15 C). Immediately following blood collection,
CO:COLLECTION_SUMMARY            	whole blood was placed on ice for 10 min while organs were harvested in
CO:COLLECTION_SUMMARY            	pre-weighed microcentrifuge tubes and snap frozen in liquid nitrogen. After
CO:COLLECTION_SUMMARY            	clotting on ice, whole blood was centrifuged at 1600 g for 10 min at 4 C. Serum
CO:COLLECTION_SUMMARY            	(top layer) was then pipetted into a new 0.5 mL microcentrifuge tube and snap
CO:COLLECTION_SUMMARY            	frozen in liquid nitrogen.
CO:SAMPLE_TYPE                   	intestine, liver, serum
#TREATMENT
TR:TREATMENT_SUMMARY             	For control treatment, conditioned facility water was autoclaved prior to
TR:TREATMENT_SUMMARY             	zebrafish exposure. For antimicrobial treatment, 2% penicillin/streptomycin, 5
TR:TREATMENT_SUMMARY             	ug/mL kanamycin, 100 ug/mL ampicillin, and 250 ng/mL amphotericin b were added
TR:TREATMENT_SUMMARY             	to autoclaved facility water. Both conditions were exposed to 10 mM
TR:TREATMENT_SUMMARY             	[U-13C]glucose.
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	For serum extractions, samples were stored on ice until the addition of 2:2:1
SP:SAMPLEPREP_SUMMARY            	methanol:acetonitrile:water (MAW) at a ratio of 1 uL serum:15 uL MAW. Samples
SP:SAMPLEPREP_SUMMARY            	were briefly mixed and immediately placed at -20 C for 1 h. Following
SP:SAMPLEPREP_SUMMARY            	incubation, samples were centrifuged at 20,000 g for 10 min at 4 C, and
SP:SAMPLEPREP_SUMMARY            	supernatant was transferred to LC-MS vials for analysis. For organ samples,
SP:SAMPLEPREP_SUMMARY            	tissues were ground in collection tubes by using a pestle while submerged in
SP:SAMPLEPREP_SUMMARY            	liquid nitrogen. Frozen samples in tubes were then massed, and the preweight of
SP:SAMPLEPREP_SUMMARY            	the tube was subtracted to yield tissue weight. 40 uL of 2:2:1 MAW were added
SP:SAMPLEPREP_SUMMARY            	per mg wet tissue weight, and the sample was vortexed before snap freezing in
SP:SAMPLEPREP_SUMMARY            	liquid nitrogen. Simultaneously, samples were extracted by thawing in room
SP:SAMPLEPREP_SUMMARY            	temperature water (~10 s), sonicating (5 min), vortexing (~30 s), snap freezing
SP:SAMPLEPREP_SUMMARY            	in liquid nitrogen (~1 min), thawing in room temperature water (~10 s),
SP:SAMPLEPREP_SUMMARY            	sonicating (5 min), and vortexing (~30 s) before incubating at -20 C for 1 h.
SP:SAMPLEPREP_SUMMARY            	Following incubation, samples were centrifuged at 20,000 g for 10 min at 4 C,
SP:SAMPLEPREP_SUMMARY            	and supernatant was transferred to LC-MS vials for analysis.
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_TYPE           	HILIC
CH:INSTRUMENT_NAME               	Thermo Vanquish
CH:COLUMN_NAME                   	HILICON iHILIC-(P) Classic (100 x 2.1mm,5um)
CH:SOLVENT_A                     	95% water/5% acetonitrile; 20 mM ammonium acetate; 0.1% ammonium hydroxide; 5
CH:SOLVENT_A                     	μM medronic acid
CH:SOLVENT_B                     	95% acetonitrile/5% water
CH:FLOW_GRADIENT                 	0-0.5 min, 90% solvent B at 200 μL/min; 0.5-17.5 min, linear gradient from 90%
CH:FLOW_GRADIENT                 	solvent B to 45% solvent B at 200 μL/min; 17.5-19 min, from 45% solvent B to
CH:FLOW_GRADIENT                 	30% solvent B at 200 μL/min; 19-19.5 min, held at 30% solvent B at 200 μL/min;
CH:FLOW_GRADIENT                 	19.5-22 min, from 30% solvent B to 90% solvent B at 200 μL/min; 22-23 min, 90%
CH:FLOW_GRADIENT                 	solvent B at 400 μL/min; 23-31.5 min, held at 90% solvent B at 400 μL/min;
CH:FLOW_GRADIENT                 	31.5-32.5 min, ramped down to 90% solvent B at 200 μL/min; 32.5-34 min, held at
CH:FLOW_GRADIENT                 	90% solvent B at 200 μL/min
CH:FLOW_RATE                     	0-22 min, 200 μL/min; 22-31.5 min, 400 μL/min; 31.5-34 min, 200 μL/min
CH:COLUMN_TEMPERATURE            	40
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
#MS
MS:INSTRUMENT_NAME               	Thermo Orbitrap ID-X Tribrid
MS:INSTRUMENT_TYPE               	Orbitrap
MS:MS_TYPE                       	ESI
MS:ION_MODE                      	NEGATIVE
MS:MS_COMMENTS                   	MS was acquired in full-scan mode from 67-525 m/z using the following source
MS:MS_COMMENTS                   	settings: spray voltage, -3.5 kV; sheath gas flow rate, 50; auxiliary gas flow
MS:MS_COMMENTS                   	rate, 10; sweep gas flow rate, 1; RF lens, 35%; ion transfer tube temperature,
MS:MS_COMMENTS                   	300 °C; and vaporizer temperature, 200 °C. Skyline software was used for data
MS:MS_COMMENTS                   	processing.
#MS_METABOLITE_DATA
MS_METABOLITE_DATA:UNITS	Percent Labeling
MS_METABOLITE_DATA_START
Samples	UGlucose_Neg_Ctrl_16_Serum	UGlucose_Neg_Ctrl_17_Serum	UGlucose_Neg_Ctrl_18_Serum	UGlucose_Neg_Ctrl_19_Serum	UGlucose_Neg_Ctrl_20_Serum	UGlucose_Neg_Ctrl_21_Serum	UGlucose_Neg_Ctrl_22_Serum	UGlucose_Neg_Ctrl_23_Serum	UGlucose_Neg_Anti_24_Serum	UGlucose_Neg_Anti_26_Serum	UGlucose_Neg_Anti_27_Serum	UGlucose_Neg_Anti_28_Serum	UGlucose_Neg_Anti_29_Serum	UGlucose_Neg_Anti_30_Serum	UGlucose_Neg_Anti_31_Serum	UGlucose_Neg_Ctrl_16_Liver	UGlucose_Neg_Ctrl_17_Liver	UGlucose_Neg_Ctrl_18_Liver	UGlucose_Neg_Ctrl_19_Liver	UGlucose_Neg_Ctrl_20_Liver	UGlucose_Neg_Ctrl_21_Liver	UGlucose_Neg_Ctrl_23_Liver	UGlucose_Neg_Anti_24_Liver	UGlucose_Neg_Anti_26_Liver	UGlucose_Neg_Anti_27_Liver	UGlucose_Neg_Anti_28_Liver	UGlucose_Neg_Anti_29_Liver	UGlucose_Neg_Anti_30_Liver	UGlucose_Neg_Anti_31_Liver	UGlucose_Neg_Ctrl_16_Intestine	UGlucose_Neg_Ctrl_17_Intestine	UGlucose_Neg_Ctrl_18_Intestine	UGlucose_Neg_Ctrl_19_Intestine	UGlucose_Neg_Ctrl_20_Intestine	UGlucose_Neg_Ctrl_21_Intestine	UGlucose_Neg_Ctrl_22_Intestine	UGlucose_Neg_Ctrl_23_Intestine	UGlucose_Neg_Anti_24_Intestine	UGlucose_Neg_Anti_26_Intestine	UGlucose_Neg_Anti_27_Intestine	UGlucose_Neg_Anti_28_Intestine	UGlucose_Neg_Anti_29_Intestine	UGlucose_Neg_Anti_30_Intestine	UGlucose_Neg_Anti_31_Intestine
Factors	Treatment:Control | Sample source:Serum	Treatment:Control | Sample source:Serum	Treatment:Control | Sample source:Serum	Treatment:Control | Sample source:Serum	Treatment:Control | Sample source:Serum	Treatment:Control | Sample source:Serum	Treatment:Control | Sample source:Serum	Treatment:Control | Sample source:Serum	Treatment:Antimicrobials | Sample source:Serum	Treatment:Antimicrobials | Sample source:Serum	Treatment:Antimicrobials | Sample source:Serum	Treatment:Antimicrobials | Sample source:Serum	Treatment:Antimicrobials | Sample source:Serum	Treatment:Antimicrobials | Sample source:Serum	Treatment:Antimicrobials | Sample source:Serum	Treatment:Control | Sample source:Liver	Treatment:Control | Sample source:Liver	Treatment:Control | Sample source:Liver	Treatment:Control | Sample source:Liver	Treatment:Control | Sample source:Liver	Treatment:Control | Sample source:Liver	Treatment:Control | Sample source:Liver	Treatment:Antimicrobials | Sample source:Liver	Treatment:Antimicrobials | Sample source:Liver	Treatment:Antimicrobials | Sample source:Liver	Treatment:Antimicrobials | Sample source:Liver	Treatment:Antimicrobials | Sample source:Liver	Treatment:Antimicrobials | Sample source:Liver	Treatment:Antimicrobials | Sample source:Liver	Treatment:Control | Sample source:Intestine	Treatment:Control | Sample source:Intestine	Treatment:Control | Sample source:Intestine	Treatment:Control | Sample source:Intestine	Treatment:Control | Sample source:Intestine	Treatment:Control | Sample source:Intestine	Treatment:Control | Sample source:Intestine	Treatment:Control | Sample source:Intestine	Treatment:Antimicrobials | Sample source:Intestine	Treatment:Antimicrobials | Sample source:Intestine	Treatment:Antimicrobials | Sample source:Intestine	Treatment:Antimicrobials | Sample source:Intestine	Treatment:Antimicrobials | Sample source:Intestine	Treatment:Antimicrobials | Sample source:Intestine	Treatment:Antimicrobials | Sample source:Intestine
Alanine_M0	97.42729265	96.41884239	97.54379186	96.85183578	98.63024534	97.88498377	98.00341151	98.38331283	97.94943758	97.25645525	97.4650711	98.31330365	98.98554837	97.95404066	97.76683011	96.83103981	97.320685	96.96973743	94.93718916	98.54917646	98.21354871	98.43772958	93.99577244	96.43117424	97.34761801	97.78341568	98.75392808	98.3674693	96.13202152	99.22947873	98.14129447	97.79579264	97.62337403	98.55062057	99.14893331	98.70808827	98.52263339	97.37407717	97.38891357	98.75250156	99.01045887	99.44294199	98.60149667	97.26737059
Alanine_M1	1.566830497	2.161607185	1.319442133	1.973962922	1.133968224	1.438594273	1.610389441	1.084261019	0.646089133	0.628313283	0.729940606	0.551498553	0.566443664	0.717700521	0.789146246	2.096045731	1.78217929	1.672538215	3.405839702	1.217447047	1.480492102	1.205608788	1.376018622	0.962997286	0.807103993	0.770179758	0.499760257	0.434174584	1.081972466	0.368364932	1.186920343	1.224242774	1.429055072	1.156533802	0.571323322	1.057566753	1.046551594	0.672888382	0.348510601	0.273336101	0.215316976	0.217523689	0.573235043	0.257206808
Alanine_M2	0.106474913	0.290327224	0.174495302	0.235408203	0.03644401	0.075949609	0.046745014	0.077589241	0.12971432	0.348437232	0.239916236	0.083088284	0.028140708	0.175937666	0.276553157	0.134644979	0.20281094	0.23519788	0.309130246	0.002930343	0.053029654	0.067325056	0.526217439	0.405283558	0.273573388	0.169864639	0.145615544	0.217915573	0.512764045	0.04726283	0.138597117	0.163413278	0.167629746	0.026772293	0.041938473	0.037690924	0.058705479	0.161821864	0.36814321	0.147716109	0.061699253	0.034831887	0.091455262	0.248011968
Alanine_M3	0.89940194	1.129223201	0.962270703	0.938793094	0.199342431	0.600472343	0.339454034	0.454836907	1.274758963	1.766794233	1.565072062	1.052109512	0.419867254	1.152321152	1.167470486	0.938269484	0.694324769	1.12252647	1.347840895	0.230446153	0.252929537	0.289336573	4.101991503	2.20054492	1.571704605	1.276539924	0.600696117	0.980440545	2.273241973	0.354893511	0.533188075	0.81655131	0.779941155	0.266073332	0.237804894	0.196654048	0.372109532	1.791212583	1.894432621	0.826446232	0.712524904	0.304702436	0.733813022	2.227410636
Hexose Phosphate_M0	100	100	100	100	100	100	100	100	100	100	100	100	99.96372837	100	99.57672751	99.94185468	98.06846274	97.86728704	97.32914961	93.90616384	97.1944505	99.02384998	98.10186408	99.50485494	98.45604302	99.42957048	99.73473473	98.92704104	99.05854652	97.0037944	91.75370598	96.01532576	90.93715382	91.8949734	94.99596302	96.11570214	96.8743343	91.54582897	97.41319298	97.3917748	95.84768167	99.22825156	88.4710933	91.4280128
Hexose Phosphate_M1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1.250264662	0.949545561	2.670850393	6.09383616	2.727448808	0.92320866	0	0	0	0	0	0	0	2.996205597	7.490880763	3.079187659	8.478376634	8.105026602	4.949240489	3.667934885	2.89804545	2.280466145	0	0.934789148	1.88059006	0.221007434	1.775628696	1.56310745
Hexose Phosphate_M2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Hexose Phosphate_M3	0	0	0	0	0	0	0	0	0	0	0	0	0.036271632	0	0.423272492	0	0.414932961	0.775380911	0	0	0	0	0.810449765	0.426089982	0.755714614	0.145803563	0.102224971	0.221434656	0.609841905	0	0.755413259	0.680605564	0.409202078	0	0	0.190097209	0.143537385	3.883233074	1.190149013	1.274928976	1.33680268	0.375014822	3.388957191	2.680519072
Hexose Phosphate_M4	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0.033813786	0	0	0	0	0	0	0	0	0	0.054796492	0	0	0	0	0	0	0	0	0
Hexose Phosphate_M5	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Hexose Phosphate_M6	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0.058145321	0.266339633	0.407786491	0	0	0.078100694	0.05294136	1.087686154	0.069055075	0.754428578	0.424625953	0.163040301	0.851524308	0.331611576	0	0	0.224881018	0.175267468	0	0	0.026265766	0.084082868	2.290471807	1.396658006	0.398507081	0.934925593	0.175726187	6.364320809	4.328360675
Fructose_M0	85.01307263	76.15351736	85.35246713	75.35819426	85.05454679	90.33784705	85.71268834	79.37689793	82.43270912	76.94547366	90.49701736	90.77937257	91.4324217	75.63506699	90.38984853	84.78380676	78.30689374	93.62494527	59.73254577	90.93063657	89.1148185	95.17009928	82.71373084	94.42907204	91.46379832	95.02348192	98.34299543	91.36028977	94.02417147	88.49221317	60.7590643	83.54197601	77.8372872	85.87727919	88.90539812	88.12237353	71.83543012	56.96490783	84.35467604	89.2857755	94.34313282	96.29094666	64.98506679	86.16396624
Fructose_M1	9.355248315	12.16797331	6.453250475	12.18194361	10.65421738	6.735118039	10.70049389	9.948008046	3.14556447	2.819746066	2.655915912	2.434656298	2.944673738	6.465842064	2.684517269	13.24255105	14.17849046	1.233999359	34.20015989	5.349600446	6.234449214	1.959134542	3.87075118	0	1.476318182	0	0	0.174691677	0.886866974	6.350547708	25.26842526	7.95796377	16.56940271	9.988725112	9.324030334	10.52575139	10.82749506	14.71589669	2.935566446	3.581022573	0	0	9.35606622	4.006885657
Fructose_M2	1.88611764	4.844612882	2.645164747	4.790016197	1.121608654	1.120215248	1.925229151	3.792933215	3.751831027	5.795639658	2.370827508	1.837268892	1.610377154	4.773829197	1.888933969	0.28316433	5.357539612	1.287170647	3.419575063	3.719762983	2.86245061	0.357799217	2.222582783	4.013907748	2.206928694	1.166916041	0.447704798	1.703208826	1.68078055	2.347778204	4.944969868	2.841409961	4.179439509	1.524935098	0.516039681	0.2048273	2.48973224	5.355026771	1.819000994	1.405178301	2.001996508	2.39306056	2.814386142	0.200469954
Fructose_M3	2.477111568	3.209438895	3.647130139	4.627599468	2.300950839	1.38424502	1.346098456	3.914581584	6.966426163	8.487330522	2.881861507	3.024974835	2.941550021	8.022605113	3.347803798	0.463947448	0	3.043952625	0	0	1.25143895	1.82416806	9.057201217	0	3.582834176	3.020724881	0.636656861	3.934673519	3.408181002	1.570494932	8.223597844	5.064045022	0.697417162	1.368358521	1.254531864	1.049462531	12.2575383	17.46486545	6.022132999	4.117425125	3.196279557	1.31599278	14.90747735	4.635092459
Fructose_M4	0	0.736630965	0.117355663	0.352131045	0	0.008792016	0	0.590493335	0.151702486	0	0.014977104	0	0	0.47291947	0.016497525	0	0	0	0	0	0	0.14789749	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0.147870581	0	0	0	0	0	0	0
Fructose_M5	0.066446777	0.111107189	0.087742317	0.136931753	0.367804297	0.107209064	0.076409122	0.026546321	0.113661378	0	0.045980777	0.196306533	0.016504672	0	0	0.261567566	0	0	2.64771927	0	0	0.161197263	0.166524738	0	0	0	0.045328972	0.502403473	0	0.194332409	0	0	0.716453417	1.240702084	0	0.097585243	0	0.370345632	0	0.060018751	0	0	0	0
Fructose_M6	1.20200307	2.776719393	1.696889533	2.553183664	0.50087204	0.306573564	0.239081041	2.350539564	3.438105356	5.951810093	1.533419832	1.72742087	1.054472714	4.629737165	1.672398907	0.964962848	2.157076189	0.809932104	0	0	0.536842729	0.379704151	1.969209246	1.557020215	1.270120626	0.788877161	0.527313938	2.324732731	0	1.044633575	0.803942734	0.594605236	0	0	0	0	2.441933702	5.128957621	4.868623521	1.550579754	0.458591115	0	7.937003489	4.993585692
Glucose_M0	88.71266823	79.73865835	88.88154171	78.62584195	80.90724624	87.11210591	85.39831015	85.88307999	83.35779239	88.60127583	94.02043404	88.92072405	94.53512409	81.21717557	86.13196672	94.84587903	90.13054202	91.0575769	88.44568117	85.1928759	89.93533729	95.64652201	92.57676596	92.6040327	95.22777997	93.45346051	96.88630088	91.89102013	91.11719082	90.1430139	83.29496566	91.28664257	79.25893528	82.63420783	87.45636764	88.57456181	90.06830689	84.26083039	89.90305996	93.27779818	87.31512289	93.4362807	78.66262682	64.12622439
Glucose_M1	5.657247067	9.759957777	3.459028288	11.33695375	14.18490281	7.57572699	10.6350268	7.721258307	0.649073868	0.263708013	0.136615725	0.238403169	0.094025323	0.76262378	1.074363283	2.697038557	3.907705067	2.996237924	5.976485401	11.12833192	6.021500922	2.082603573	0	0	0	0	0	0	0.830354398	4.76727968	7.766260978	2.319991766	10.08943547	12.43205068	6.737679024	7.85310094	5.085767596	0.420634298	0	0	0.591208839	0.082658608	0.224488288	0.694790069
Glucose_M2	0.954241695	3.480758363	1.368504759	3.290740487	2.288787211	1.28542716	1.683021694	1.695099213	1.350287909	2.388836307	1.073675829	1.016247683	0.916244026	1.861273099	1.849355574	0.515727693	2.077781715	1.089507322	2.151198722	1.90740028	1.122189219	0.597377461	0.682559833	0.892931593	0.928017959	0.655999983	0.558607686	0.974430448	1.503685552	0.828045341	2.635810197	1.199304843	3.516877949	2.123316531	1.187153677	1.320018652	1.164306407	0.866749385	1.353187057	0.946582726	1.039634049	0.492255694	1.39392088	1.267821613
Glucose_M3	0.904715253	2.343105404	1.689542547	2.012919399	1.04264157	0.864117122	0.632142498	1.071274636	2.595213798	1.633915228	1.114967283	1.24265083	1.025141316	1.881263174	1.764311571	0.437374696	1.409144033	1.52064025	1.405903125	0.731800704	0.610056689	0.507768733	1.625661376	0.576809766	1.030285857	0.889319769	0.677684563	1.03632512	1.430986143	0.726915517	2.04553596	1.447991398	2.169359435	0.735338869	0.72794602	0.442015347	0.809564089	2.223794378	1.061113855	1.082002709	1.412593933	0.984608093	1.55428287	1.634552972
Glucose_M4	0.051165177	0.39775089	0.173773402	0.328545847	0.086459299	0.019612439	0.028374632	0.140937206	0.18924908	0.076827886	0.051734908	0.047881977	0.045013723	0.092252259	0.03641	0.002470408	0.093572354	0.058863855	0.020384777	0	0.008873134	0.023629156	0.081033642	0	0.036174187	0	0	0.026242018	0	0	0.17332454	0.071678556	0.198395467	0	0	0	0.026507063	0	0	0	0.00290542	0	0.018100193	0
Glucose_M5	0	0.054903293	0.019775238	0.029670943	0	0	0	0.005115611	0	0.002103285	0	0.01045393	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0.019789338	0	0	0	0	0	0.010835795	0	0	0	0	0	0
Glucose_M6	3.719962579	4.224865921	4.407834055	4.375327623	1.489962872	3.143010383	1.623124228	3.483235034	11.85838296	7.033333455	3.602572216	8.523638364	3.384451526	14.18541212	9.143592849	1.501509617	2.381254812	3.277173747	2.000346809	1.039591198	2.302042743	1.142099064	5.033979187	5.926225939	2.777742025	5.001219735	1.877406872	6.07198228	5.117783086	3.534745564	4.084102668	3.654601532	4.766996405	2.075086094	3.89085364	1.810303254	2.845547951	12.21715575	7.682639125	4.693616386	9.63853487	5.004196906	18.14658095	32.27661096
Lactate_M0	95.36683371	91.36864959	92.16535726	91.98452553	93.83364579	93.67934075	96.57139286	94.57374199	93.5788322	93.36579172	92.67568854	93.73225038	97.28974345	95.64608303	95.59827163	93.32017728	91.31632844	91.94546264	90.1028793	91.54123337	92.56043878	94.63537021	90.91265642	87.85873	93.9162863	95.47203156	97.78530694	95.4768232	94.39974314	91.90823725	88.68926999	90.87678634	90.06940043	90.4350618	92.50241695	93.84972796	92.47406188	83.58414574	85.51324746	91.75037897	91.90909401	96.08607063	87.79437515	86.14689427
Lactate_M1	2.335095615	4.43457444	3.282339342	4.408373874	4.547932247	3.895498429	2.473244633	3.02626731	1.247746901	1.268850383	1.533809457	1.171221453	0.796883811	0.938112684	1.14716187	4.219714873	5.223125539	3.753687605	6.565655165	7.243532211	5.477588126	3.779439896	1.766288213	1.754548563	1.355393518	1.040834679	0.722935408	1.119822846	1.309522273	3.800818481	5.842575469	3.822204767	5.307506264	6.968890656	4.351577061	4.363975759	4.008448683	2.217506466	2.030708886	1.753734101	2.019900664	1.128446593	2.154706209	1.704054344
Lactate_M2	0.265677359	0.802668581	0.717383753	0.712508146	0.325192059	0.364480322	0.173872936	0.392076224	0.46162109	0.827896816	0.751426592	0.411827978	0.169859768	0.303899431	0.49631939	0.368890855	0.85470886	0.681142917	0.865435323	0.338318667	0.354899029	0.359328073	0.806685872	1.430336107	0.712304836	0.42617414	0.242599454	0.627946184	0.806566178	0.575018753	1.190195252	0.944278195	1.035907861	0.621603012	0.42224156	0.334328191	0.617022086	1.124676856	1.54684346	0.922085286	0.764916956	0.390056057	1.108853554	0.959597353
Lactate_M3	2.032393316	3.394107388	3.834919647	2.89459245	1.293229909	2.060680503	0.781489573	2.007914475	4.711799813	4.537461084	5.039075412	4.684700187	1.74351297	3.111904858	2.758247109	2.091216993	2.605837157	3.619706841	2.466030216	0.876915747	1.607074063	1.225861816	6.514369495	8.956385335	4.016015342	3.060959625	1.249158196	2.775407766	3.484168406	3.715925511	4.277959289	4.356730703	3.587185442	1.974444534	2.723764432	1.451968089	2.900467351	13.07367094	10.9092002	5.573801641	5.30608837	2.395426716	8.942065084	11.18945404
Sorbitol_M0	98.10568263	92.10828244	96.94587888	92.55723858	95.08460342	96.38267922	95.48268778	94.51430167	93.84073182	83.15032871	79.10153697	97.57564133	98.83460451	74.39666403	79.47184953	97.98385182	92.84894445	97.3286625	92.1453721	96.63599175	96.33369779	98.57628604	95.69035583	42.67429038	69.35506801	96.63705165	97.0050681	80.89994886	48.64218039	98.39347536	95.2027284	95.55487806	94.73092745	96.77738954	98.04715304	95.89344613	97.12448889	90.82671889	27.72161915	36.91649339	95.03326175	99.24645909	70.10036044	12.0017409
Sorbitol_M1	0.024559631	3.529298045	0.330857567	4.065570299	1.888467346	0.174951893	2.692979882	1.303459351	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0.429582637	3.199432592	1.59845181	3.007672383	1.689557247	1.023611794	3.290626151	0.910055684	0.789636082	0	0.038521977	0	0	0	0.036900813
Sorbitol_M2	0.422437815	1.4380457	0.784306846	1.697623651	1.851084479	1.601368556	1.458461989	1.064371867	0.694409697	0.411090128	0.675209689	0.526245716	0.357485623	0.95026176	1.370333548	1.069344471	4.937388578	0.463851289	5.207895965	2.062870111	1.23967282	0.627916069	0.568039654	0.113414484	0.401888709	0.560297621	1.207963941	0.723835609	1.289430382	0.230394526	0.492792629	0.524457494	0.551243668	0.907862904	0.290380725	0.294551009	0.27288733	0.4581865	0.399636493	0.264841591	0.068795124	0.258463078	0.297394204	0
Sorbitol_M3	0.098740327	0.347578102	0.356862617	0.100330352	0	0.062274773	0	0	0.906770554	0.114388781	0.376537408	0.02793595	0.160590181	0	0	0	0	0.209972627	0	0	0	0.073416429	0.100349781	0.025898592	0	0	0	0	0	0.056363861	0.113927089	0.712003821	0.143916636	0	0.095784099	0	0.106170557	1.241544444	0.057860798	0.165663247	0.157569836	0	0.197801574	0.182127621
Sorbitol_M4	0	0	0.046222806	0.051799498	0	0	0.192907935	0.078410515	0.014433478	0	0	0	0	0	0	0.036501128	0	0	0	0.028941831	0.777681966	0	0	0.174519311	0	0.152441524	0.151919628	0	0.262405635	0.028305224	0.135659476	0	0.150784666	0	0.005278763	0.004413258	0	0	0	0	0.017701208	0.044407324	0	0.037877748
Sorbitol_M5	1.086629579	1.590632897	0	0.637124756	0.821152794	1.626699173	0.17296242	1.589981189	0.268859839	0.422288908	0.120824863	0.402669613	0.572233893	0.298525637	0	0.910302585	2.213666977	1.768024328	2.646731931	1.272196309	1.109570573	0.722381463	1.43317093	0.601185036	0.266756973	0.895118925	1.63504833	0.294829448	0.8839584	0.067916166	0.492092669	0.29438878	0.153965378	0.435321156	0.096431315	0.03944999	0.646818268	0	0.285369288	0.493488605	0.184178866	0.246079402	0.078115432	0.440705689
Sorbitol_M6	0.261950018	0.986162811	1.535871285	0.89031286	0.354691963	0.152026387	0	1.449475406	4.274794617	15.90190348	19.72589107	1.467507389	0.075085789	24.35454857	19.15781692	0	0	0.229489259	0	0	0.539376851	0	2.208083807	56.41069219	29.97628631	1.755090278	0	18.08138608	48.92202519	0.793962227	0.363367147	1.315820037	1.261489819	0.189869156	0.44136026	0.477513463	0.939579268	6.683914082	71.53551427	62.12099119	4.538493219	0.204591108	29.32632835	87.30064723
MS_METABOLITE_DATA_END
#METABOLITES
METABOLITES_START
metabolite_name
Alanine_M0
Alanine_M1
Alanine_M2
Alanine_M3
Hexose Phosphate_M0
Hexose Phosphate_M1
Hexose Phosphate_M2
Hexose Phosphate_M3
Hexose Phosphate_M4
Hexose Phosphate_M5
Hexose Phosphate_M6
Fructose_M0
Fructose_M1
Fructose_M2
Fructose_M3
Fructose_M4
Fructose_M5
Fructose_M6
Glucose_M0
Glucose_M1
Glucose_M2
Glucose_M3
Glucose_M4
Glucose_M5
Glucose_M6
Lactate_M0
Lactate_M1
Lactate_M2
Lactate_M3
Sorbitol_M0
Sorbitol_M1
Sorbitol_M2
Sorbitol_M3
Sorbitol_M4
Sorbitol_M5
Sorbitol_M6
METABOLITES_END
#END