#METABOLOMICS WORKBENCH mjackstadt1_20250701_123241 DATATRACK_ID:6125 STUDY_ID:ST004076 ANALYSIS_ID:AN006746 PROJECT_ID:PR002557
VERSION             	1
CREATED_ON             	July 24, 2025, 11:47 am
#PROJECT
PR:PROJECT_TITLE                 	Glucose-derived sorbitol drives steatotic liver disease in the absence of gut
PR:PROJECT_TITLE                 	bacteria
PR:PROJECT_SUMMARY               	The role of the gut microbiome in mediating steatotic liver disease is poorly
PR:PROJECT_SUMMARY               	understood. Here, we developed a model to deplete the gut microbiome in adult
PR:PROJECT_SUMMARY               	zebrafish and discovered that it led to steatotic liver disease in animals on
PR:PROJECT_SUMMARY               	standard diets. Using metabolomics and isotope tracing, we found that dietary
PR:PROJECT_SUMMARY               	glucose is transformed into sorbitol within the intestine. While bacteria
PR:PROJECT_SUMMARY               	degrade the sorbitol in control animals, sorbitol is passed to the livers of
PR:PROJECT_SUMMARY               	fish in which the gut microbiome has been depleted. Within the liver, sorbitol
PR:PROJECT_SUMMARY               	is converted into fructose 1-phosphate, which subsequently activates
PR:PROJECT_SUMMARY               	glucokinase. The result is increased hepatic metabolism of glucose, leading to
PR:PROJECT_SUMMARY               	elevated glycogen and fat content. Inhibition of sorbitol production in
PR:PROJECT_SUMMARY               	microbiome-depleted animals was sufficient to prevent the development of
PR:PROJECT_SUMMARY               	steatotic liver, and colonizing sorbitol-degrading bacteria in the intestines of
PR:PROJECT_SUMMARY               	microbiome-depleted fish reversed the steatotic liver phenotype. Taken together,
PR:PROJECT_SUMMARY               	these findings show that sorbitol-degrading bacteria in the gut protect against
PR:PROJECT_SUMMARY               	steatotic liver disease.
PR:INSTITUTE                     	Washington University in St. Louis
PR:LAST_NAME                     	Jackstadt
PR:FIRST_NAME                    	Madelyn
PR:ADDRESS                       	One Brookings Drive, St. Louis, Missouri, 63130, USA
PR:EMAIL                         	mjackstadt@wustl.edu
PR:PHONE                         	3149356405
#STUDY
ST:STUDY_TITLE                   	[U-13C]Sorbitol Labeling in Adult Zebrafish Treated with Antimicrobials or
ST:STUDY_TITLE                   	Control
ST:STUDY_SUMMARY                 	Sorbitol metabolism was traced with [U-13C]sorbitol in the intestine and liver
ST:STUDY_SUMMARY                 	of adult zebrafish with in-tact or depleted gut microbiota. We found that fish
ST:STUDY_SUMMARY                 	treated with antimicrobials had elevated sorbitol-derived carbon in metabolites
ST:STUDY_SUMMARY                 	in these organs.
ST:INSTITUTE                     	Washington University in St. Louis
ST:LAST_NAME                     	Jackstadt
ST:FIRST_NAME                    	Madelyn
ST:ADDRESS                       	One Brookings Drive, St. Louis, Missouri, 63130, USA
ST:EMAIL                         	mjackstadt@wustl.edu
ST:PHONE                         	3149356405
#SUBJECT
SU:SUBJECT_TYPE                  	Fish
SU:SUBJECT_SPECIES               	Danio rerio
SU:TAXONOMY_ID                   	7955
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data
SUBJECT_SAMPLE_FACTORS           	-	USorbitol_Neg_Anti_01_Intestine	Treatment:Antimicrobials | Sample source:Intestine	RAW_FILE_NAME(Raw File Name)=USorbitol_Neg_Anti_01_Intestine.mzML
SUBJECT_SAMPLE_FACTORS           	-	USorbitol_Neg_Anti_02_Intestine	Treatment:Antimicrobials | Sample source:Intestine	RAW_FILE_NAME(Raw File Name)=USorbitol_Neg_Anti_02_Intestine.mzML
SUBJECT_SAMPLE_FACTORS           	-	USorbitol_Neg_Anti_03_Intestine	Treatment:Antimicrobials | Sample source:Intestine	RAW_FILE_NAME(Raw File Name)=USorbitol_Neg_Anti_03_Intestine.mzML
SUBJECT_SAMPLE_FACTORS           	-	USorbitol_Neg_Anti_04_Intestine	Treatment:Antimicrobials | Sample source:Intestine	RAW_FILE_NAME(Raw File Name)=USorbitol_Neg_Anti_04_Intestine.mzML
SUBJECT_SAMPLE_FACTORS           	-	USorbitol_Neg_Anti_06_Intestine	Treatment:Antimicrobials | Sample source:Intestine	RAW_FILE_NAME(Raw File Name)=USorbitol_Neg_Anti_06_Intestine.mzML
SUBJECT_SAMPLE_FACTORS           	-	USorbitol_Neg_Ctrl_07_Intestine	Treatment:Control | Sample source:Intestine	RAW_FILE_NAME(Raw File Name)=USorbitol_Neg_Ctrl_07_Intestine.mzML
SUBJECT_SAMPLE_FACTORS           	-	USorbitol_Neg_Ctrl_08_Intestine	Treatment:Control | Sample source:Intestine	RAW_FILE_NAME(Raw File Name)=USorbitol_Neg_Ctrl_08_Intestine.mzML
SUBJECT_SAMPLE_FACTORS           	-	USorbitol_Neg_Ctrl_09_Intestine	Treatment:Control | Sample source:Intestine	RAW_FILE_NAME(Raw File Name)=USorbitol_Neg_Ctrl_09_Intestine.mzML
SUBJECT_SAMPLE_FACTORS           	-	USorbitol_Neg_Ctrl_10_Intestine	Treatment:Control | Sample source:Intestine	RAW_FILE_NAME(Raw File Name)=USorbitol_Neg_Ctrl_10_Intestine.mzML
SUBJECT_SAMPLE_FACTORS           	-	USorbitol_Neg_Ctrl_11_Intestine	Treatment:Control | Sample source:Intestine	RAW_FILE_NAME(Raw File Name)=USorbitol_Neg_Ctrl_11_Intestine.mzML
SUBJECT_SAMPLE_FACTORS           	-	USorbitol_Neg_Ctrl_12_Intestine	Treatment:Control | Sample source:Intestine	RAW_FILE_NAME(Raw File Name)=USorbitol_Neg_Ctrl_12_Intestine.mzML
SUBJECT_SAMPLE_FACTORS           	-	USorbitol_Neg_Anti_01_Liver	Treatment:Antimicrobials | Sample source:Liver	RAW_FILE_NAME(Raw File Name)=USorbitol_Neg_Anti_01_Liver.mzML
SUBJECT_SAMPLE_FACTORS           	-	USorbitol_Neg_Anti_02_Liver	Treatment:Antimicrobials | Sample source:Liver	RAW_FILE_NAME(Raw File Name)=USorbitol_Neg_Anti_02_Liver.mzML
SUBJECT_SAMPLE_FACTORS           	-	USorbitol_Neg_Anti_03_Liver	Treatment:Antimicrobials | Sample source:Liver	RAW_FILE_NAME(Raw File Name)=USorbitol_Neg_Anti_03_Liver.mzML
SUBJECT_SAMPLE_FACTORS           	-	USorbitol_Neg_Anti_04_Liver	Treatment:Antimicrobials | Sample source:Liver	RAW_FILE_NAME(Raw File Name)=USorbitol_Neg_Anti_04_Liver.mzML
SUBJECT_SAMPLE_FACTORS           	-	USorbitol_Neg_Anti_06_Liver	Treatment:Antimicrobials | Sample source:Liver	RAW_FILE_NAME(Raw File Name)=USorbitol_Neg_Anti_06_Liver.mzML
SUBJECT_SAMPLE_FACTORS           	-	USorbitol_Neg_Ctrl_07_Liver	Treatment:Control | Sample source:Liver	RAW_FILE_NAME(Raw File Name)=USorbitol_Neg_Ctrl_07_Liver.mzML
SUBJECT_SAMPLE_FACTORS           	-	USorbitol_Neg_Ctrl_08_Liver	Treatment:Control | Sample source:Liver	RAW_FILE_NAME(Raw File Name)=USorbitol_Neg_Ctrl_08_Liver.mzML
SUBJECT_SAMPLE_FACTORS           	-	USorbitol_Neg_Ctrl_09_Liver	Treatment:Control | Sample source:Liver	RAW_FILE_NAME(Raw File Name)=USorbitol_Neg_Ctrl_09_Liver.mzML
SUBJECT_SAMPLE_FACTORS           	-	USorbitol_Neg_Ctrl_10_Liver	Treatment:Control | Sample source:Liver	RAW_FILE_NAME(Raw File Name)=USorbitol_Neg_Ctrl_10_Liver.mzML
SUBJECT_SAMPLE_FACTORS           	-	USorbitol_Neg_Ctrl_11_Liver	Treatment:Control | Sample source:Liver	RAW_FILE_NAME(Raw File Name)=USorbitol_Neg_Ctrl_11_Liver.mzML
SUBJECT_SAMPLE_FACTORS           	-	USorbitol_Neg_Ctrl_12_Liver	Treatment:Control | Sample source:Liver	RAW_FILE_NAME(Raw File Name)=USorbitol_Neg_Ctrl_12_Liver.mzML
#COLLECTION
CO:COLLECTION_SUMMARY            	Zebrafish were anesthetized and thoroughly dried before a scalpel was used to
CO:COLLECTION_SUMMARY            	excise the caudal fin and some attached muscle tissue. Zebrafish were then
CO:COLLECTION_SUMMARY            	immediately placed in a spin column such that blood from the wound could flow
CO:COLLECTION_SUMMARY            	from the fish (in a 1.5 mL microcentrifuge tube with a small hole in the bottom)
CO:COLLECTION_SUMMARY            	to a collection tube (a 0.5 mL microcentrifuge tube) during a brief, low-speed
CO:COLLECTION_SUMMARY            	centrifugation (40 g for 1 min at 15 C). Immediately following blood collection,
CO:COLLECTION_SUMMARY            	whole blood was placed on ice for 10 min while organs were harvested in
CO:COLLECTION_SUMMARY            	pre-weighed microcentrifuge tubes and snap frozen in liquid nitrogen. After
CO:COLLECTION_SUMMARY            	clotting on ice, whole blood was centrifuged at 1600 g for 10 min at 4 C. Serum
CO:COLLECTION_SUMMARY            	(top layer) was then pipetted into a new 0.5 mL microcentrifuge tube and snap
CO:COLLECTION_SUMMARY            	frozen in liquid nitrogen.
CO:SAMPLE_TYPE                   	intestine, liver
#TREATMENT
TR:TREATMENT_SUMMARY             	For control treatment, conditioned facility water was autoclaved prior to
TR:TREATMENT_SUMMARY             	zebrafish exposure. For antimicrobial treatment, 2% penicillin/streptomycin, 5
TR:TREATMENT_SUMMARY             	ug/mL kanamycin, 100 ug/mL ampicillin, and 250 ng/mL amphotericin b were added
TR:TREATMENT_SUMMARY             	to autoclaved facility water. Both conditions were exposed to 0.1 mg/mL
TR:TREATMENT_SUMMARY             	[U-13C]sorbitol.
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	For organ samples, tissues were ground in collection tubes by using a pestle
SP:SAMPLEPREP_SUMMARY            	while submerged in liquid nitrogen. Frozen samples in tubes were then massed,
SP:SAMPLEPREP_SUMMARY            	and the preweight of the tube was subtracted to yield tissue weight. 40 uL of
SP:SAMPLEPREP_SUMMARY            	2:2:1 methanol:acetonitrile:water were added per mg wet tissue weight, and the
SP:SAMPLEPREP_SUMMARY            	sample was vortexed before snap freezing in liquid nitrogen. Simultaneously,
SP:SAMPLEPREP_SUMMARY            	samples were extracted by thawing in room temperature water (~10 s), sonicating
SP:SAMPLEPREP_SUMMARY            	(5 min), vortexing (~30 s), snap freezing in liquid nitrogen (~1 min), thawing
SP:SAMPLEPREP_SUMMARY            	in room temperature water (~10 s), sonicating (5 min), and vortexing (~30 s)
SP:SAMPLEPREP_SUMMARY            	before incubating at -20 C for 1 h. Following incubation, samples were
SP:SAMPLEPREP_SUMMARY            	centrifuged at 20,000 g for 10 min at 4 C, and supernatant was transferred to
SP:SAMPLEPREP_SUMMARY            	LC-MS vials for analysis.
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_TYPE           	HILIC
CH:INSTRUMENT_NAME               	Thermo Vanquish
CH:COLUMN_NAME                   	HILICON iHILIC-(P) Classic (100 x 2.1mm,5um)
CH:SOLVENT_A                     	95% water/5% acetonitrile; 20 mM ammonium acetate; 0.1% ammonium hydroxide; 5
CH:SOLVENT_A                     	μM medronic acid
CH:SOLVENT_B                     	95% acetonitrile/5% water
CH:FLOW_GRADIENT                 	0-0.5 min, 90% solvent B at 200 μL/min; 0.5-17.5 min, linear gradient from 90%
CH:FLOW_GRADIENT                 	solvent B to 45% solvent B at 200 μL/min; 17.5-19 min, from 45% solvent B to
CH:FLOW_GRADIENT                 	30% solvent B at 200 μL/min; 19-19.5 min, held at 30% solvent B at 200 μL/min;
CH:FLOW_GRADIENT                 	19.5-22 min, from 30% solvent B to 90% solvent B at 200 μL/min; 22-23 min, 90%
CH:FLOW_GRADIENT                 	solvent B at 400 μL/min; 23-31.5 min, held at 90% solvent B at 400 μL/min;
CH:FLOW_GRADIENT                 	31.5-32.5 min, ramped down to 90% solvent B at 200 μL/min; 32.5-34 min, held at
CH:FLOW_GRADIENT                 	90% solvent B at 200 μL/min
CH:FLOW_RATE                     	0-22 min, 200 μL/min; 22-31.5 min, 400 μL/min; 31.5-34 min, 200 μL/min
CH:COLUMN_TEMPERATURE            	40
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
#MS
MS:INSTRUMENT_NAME               	Thermo Orbitrap ID-X Tribrid
MS:INSTRUMENT_TYPE               	Orbitrap
MS:MS_TYPE                       	ESI
MS:ION_MODE                      	NEGATIVE
MS:MS_COMMENTS                   	MS was acquired in full-scan mode from 67-525 m/z using the following source
MS:MS_COMMENTS                   	settings: spray voltage, -3.5 kV; sheath gas flow rate, 50; auxiliary gas flow
MS:MS_COMMENTS                   	rate, 10; sweep gas flow rate, 1; RF lens, 35%; ion transfer tube temperature,
MS:MS_COMMENTS                   	300 °C; and vaporizer temperature, 200 °C. Skyline software was used for data
MS:MS_COMMENTS                   	processing.
#MS_METABOLITE_DATA
MS_METABOLITE_DATA:UNITS	Labeling Percentage
MS_METABOLITE_DATA_START
Samples	USorbitol_Neg_Anti_01_Intestine	USorbitol_Neg_Anti_02_Intestine	USorbitol_Neg_Anti_03_Intestine	USorbitol_Neg_Anti_04_Intestine	USorbitol_Neg_Anti_06_Intestine	USorbitol_Neg_Ctrl_07_Intestine	USorbitol_Neg_Ctrl_08_Intestine	USorbitol_Neg_Ctrl_09_Intestine	USorbitol_Neg_Ctrl_10_Intestine	USorbitol_Neg_Ctrl_11_Intestine	USorbitol_Neg_Ctrl_12_Intestine	USorbitol_Neg_Anti_01_Liver	USorbitol_Neg_Anti_02_Liver	USorbitol_Neg_Anti_03_Liver	USorbitol_Neg_Anti_04_Liver	USorbitol_Neg_Anti_06_Liver	USorbitol_Neg_Ctrl_07_Liver	USorbitol_Neg_Ctrl_08_Liver	USorbitol_Neg_Ctrl_09_Liver	USorbitol_Neg_Ctrl_10_Liver	USorbitol_Neg_Ctrl_11_Liver	USorbitol_Neg_Ctrl_12_Liver
Factors	Treatment:Antimicrobials | Sample source:Intestine	Treatment:Antimicrobials | Sample source:Intestine	Treatment:Antimicrobials | Sample source:Intestine	Treatment:Antimicrobials | Sample source:Intestine	Treatment:Antimicrobials | Sample source:Intestine	Treatment:Control | Sample source:Intestine	Treatment:Control | Sample source:Intestine	Treatment:Control | Sample source:Intestine	Treatment:Control | Sample source:Intestine	Treatment:Control | Sample source:Intestine	Treatment:Control | Sample source:Intestine	Treatment:Antimicrobials | Sample source:Liver	Treatment:Antimicrobials | Sample source:Liver	Treatment:Antimicrobials | Sample source:Liver	Treatment:Antimicrobials | Sample source:Liver	Treatment:Antimicrobials | Sample source:Liver	Treatment:Control | Sample source:Liver	Treatment:Control | Sample source:Liver	Treatment:Control | Sample source:Liver	Treatment:Control | Sample source:Liver	Treatment:Control | Sample source:Liver	Treatment:Control | Sample source:Liver
Hexose Phosphate_M0	93.78968775	93.11371802	95.01533516	88.59762274	91.24899862	98.91425291	95.16114896	94.58703534	99.58093048	93.58875531	95.79117192	99.87547895	98.57667478	97.67797451	70.08475324	87.48134265	99.95361244	99.96931662	99.70700075	99.92923077	99.72615539	99.87286579
Hexose Phosphate_M1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Hexose Phosphate_M2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Hexose Phosphate_M3	0	0	0.116111235	0.390955572	0.266425336	0	0	0	0	0.196892971	0.334333255	0	0	0.189152082	0.830194311	0.064877284	0	0	0	0	0	0.050895816
Hexose Phosphate_M4	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Hexose Phosphate_M5	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Hexose Phosphate_M6	6.210312245	6.886281983	4.868553603	11.01142169	8.484576041	1.085747089	4.838851035	5.412964664	0.419069517	6.214351717	3.874494828	0.124521053	1.423325224	2.13287341	29.08505245	12.45378007	0.046387556	0.030683383	0.292999254	0.070769229	0.273844612	0.076238391
Fructose_M0	87.29275498	98.49485335	89.40677407	96.71790802	98.64131119	98.57760626	99.49531799	96.64680276	98.47535445	94.53248526	96.19035796	79.03383722	96.37683843	82.17793626	82.61719101	98.64832736	96.39902953	99.9294762	99.77428477	99.25293746	98.6640295	99.21576948
Fructose_M1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Fructose_M2	0	0	0	0	0	0	0	0	0	0	0	0.605665521	1.13596189	0	1.28908001	0.494001256	0	0.070336013	0.089257407	0.203085921	0.628808477	0.395799479
Fructose_M3	0	0	0.02946473	0.020885623	0	0	0	0	0	0	0.075743281	0	0	0	0	0	0	0.000187783	0	0	0	0
Fructose_M4	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0.136457822	0	0	0
Fructose_M5	0	0	0	0.121810639	0.642007897	0	0.23624864	3.194694962	0.886825426	3.175915478	3.077920814	0	0	0	0	0.857671386	0	0	0	0.24876666	0	0.38843104
Fructose_M6	12.70724502	1.505146653	10.5637612	3.139395719	0.716680917	1.422393738	0.268433373	0.158502275	0.637820124	2.291599259	0.655977947	20.36049726	2.48719968	17.82206374	16.09372898	0	3.600970467	0	0	0.29520996	0.707162024	0
Lactate_M0	99.1636486	99.62020102	98.30196434	98.10561382	99.22141036	99.41750161	99.57043828	99.46306915	99.62317307	99.35724225	98.6433914	99.43062475	99.9907768	99.36036022	96.94755674	99.54119004	99.70340727	99.92911866	99.81800648	99.8336597	99.72507276	99.22026971
Lactate_M1	0.335792602	0.34432757	0.875233497	0.494083971	0.351448134	0.520904545	0.353345857	0.382607766	0.309285773	0.410880291	0.624859131	0.431349364	0	0.338139539	0.605955054	0.237562876	0.262667813	0.056998116	0.11408815	0.149948063	0.252941454	0.548735796
Lactate_M2	0	0	0.015036234	0	0	0	0	0	0	0	0.029864145	0	0	0	0	0	0	0	0	0	0	7.76312E-05
Lactate_M3	0.500558794	0.035471414	0.807765929	1.400302208	0.427141509	0.061593848	0.076215862	0.154323083	0.067541159	0.231877459	0.701885325	0.138025881	0.009223196	0.301500239	2.446488207	0.221247085	0.033924916	0.013883221	0.067905367	0.016392241	0.021985788	0.230916865
Sorbitol_M0	6.558903862	2.372284767	23.4301993	2.703444117	23.5998123	35.75078514	27.51282097	31.9233325	68.65216454	11.02250497	62.94176285	77.05297368	36.91849199	20.51948765	6.542869026	34.26471663	76.06219466	84.74727472	43.28146597	83.56085189	62.81780271	70.87057463
Sorbitol_M1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Sorbitol_M2	0	0	0	0	0.021611117	0	0	0	0	0	0	0.779932841	0.151441564	0	0.015839862	0.172184402	0.000443584	0.639642406	0.217027007	0.736821633	0.133307663	0.008591669
Sorbitol_M3	0	0	0	0	0	0	0	0	0	0	0.031433344	0	0	0	0	0	0	0	0	0	0	0
Sorbitol_M4	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Sorbitol_M5	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Sorbitol_M6	93.44109614	97.62771523	76.5698007	97.29655588	76.37857658	64.24921486	72.48717903	68.0766675	31.34783546	88.97749503	37.0268038	22.16709348	62.93006645	79.48051235	93.44129111	65.56309897	23.93736176	14.61308287	56.50150702	15.70232648	37.04888962	29.1208337
UDP-glucose_M0	98.1461038	100	98.78494577	97.12862024	98.40446595	100	99.81517745	99.78268547	100	99.2237889	97.68220248	100	99.96753536	98.53053022	92.67343439	97.75515378	100	100	99.99141806	100	100	99.57049065
UDP-glucose_M1	0	0	0	0	0.391155174	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
UDP-glucose_M2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
UDP-glucose_M3	1.8538962	0	1.215054232	2.871379761	1.193088274	0	0.184822546	0.211684659	0	0.776211099	2.317797518	0	0	1.469469783	7.27754326	2.244846224	0	0	0	0	0	0.412198603
UDP-glucose_M4	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
UDP-glucose_M5	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
UDP-glucose_M6	0	0	0	0	0	0	0	0.005629871	0	0	0	0	0	0	0.049022346	0	0	0	0	0	0	0
UDP-glucose_M7	0	0	0	0	0	0	0	0	0	0	0	0	0.032464639	0	0	0	0	0	0	0	0	0
UDP-glucose_M8	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
UDP-glucose_M9	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
UDP-glucose_M10	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
UDP-glucose_M11	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
UDP-glucose_M12	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
UDP-glucose_M13	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0.017310748
UDP-glucose_M14	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0.008581936	0	0	0
UDP-glucose_M15	0	0	0	0	0.011290597	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Glycerol 3-phosphate_M0	98.92500774	99.95005249	99.07728548	98.18391348	99.48727796	99.95459847	99.71016159	99.65918034	99.87963944	99.46748075	99.21389317	100	99.80433733	98.18768161	95.89989811	99.09289891	99.86948556	99.92447855	99.67636095	99.94124155	99.88067555	99.74661719
Glycerol 3-phosphate_M1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Glycerol 3-phosphate_M2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Glycerol 3-phosphate_M3	1.074992258	0.049947508	0.922714522	1.81608652	0.512722036	0.04540153	0.28983841	0.340819661	0.120360556	0.532519246	0.786106834	0	0.195662672	1.812318393	4.100101894	0.907101088	0.130514439	0.075521452	0.323639052	0.058758452	0.119324454	0.253382814
MS_METABOLITE_DATA_END
#METABOLITES
METABOLITES_START
metabolite_name
Hexose Phosphate_M0
Hexose Phosphate_M1
Hexose Phosphate_M2
Hexose Phosphate_M3
Hexose Phosphate_M4
Hexose Phosphate_M5
Hexose Phosphate_M6
Fructose_M0
Fructose_M1
Fructose_M2
Fructose_M3
Fructose_M4
Fructose_M5
Fructose_M6
Lactate_M0
Lactate_M1
Lactate_M2
Lactate_M3
Sorbitol_M0
Sorbitol_M1
Sorbitol_M2
Sorbitol_M3
Sorbitol_M4
Sorbitol_M5
Sorbitol_M6
UDP-glucose_M0
UDP-glucose_M1
UDP-glucose_M2
UDP-glucose_M3
UDP-glucose_M4
UDP-glucose_M5
UDP-glucose_M6
UDP-glucose_M7
UDP-glucose_M8
UDP-glucose_M9
UDP-glucose_M10
UDP-glucose_M11
UDP-glucose_M12
UDP-glucose_M13
UDP-glucose_M14
UDP-glucose_M15
Glycerol 3-phosphate_M0
Glycerol 3-phosphate_M1
Glycerol 3-phosphate_M2
Glycerol 3-phosphate_M3
METABOLITES_END
#END