#METABOLOMICS WORKBENCH jssacks_20250703_133942 DATATRACK_ID:6131 STUDY_ID:ST004081 ANALYSIS_ID:AN006758 PROJECT_ID:PR002562
VERSION             	1
CREATED_ON             	July 29, 2025, 11:44 am
#PROJECT
PR:PROJECT_TITLE                 	Remodeling of Prochlorococcus metabolism during viral infection
PR:PROJECT_TYPE                  	Marine Microbial Metabolomics
PR:PROJECT_SUMMARY               	The marine cyanobacterium Prochlorococcus is the most abundant photoautotroph in
PR:PROJECT_SUMMARY               	the world and is a major contributor to oceanic primary productivity. Viruses
PR:PROJECT_SUMMARY               	are important controls on Prochlorococcus populations with up to 10% of
PR:PROJECT_SUMMARY               	Prochlorococcus cells infected. During infection, viruses remodel their host’s
PR:PROJECT_SUMMARY               	metabolic machinery, creating metabolically distinct cells, termed virocells.
PR:PROJECT_SUMMARY               	However, the specific consequences of viral infection on Prochlorococcus
PR:PROJECT_SUMMARY               	metabolism remain poorly understood. Here, we characterize the infection of
PR:PROJECT_SUMMARY               	non-axenic cultures of Prochlorococcus MED4 by the T7-like virus P-SSP7 using a
PR:PROJECT_SUMMARY               	combination of metabolomics, transcriptomics, and population modeling
PR:PROJECT_SUMMARY               	approaches. P-SSP7 infection dramatically altered the metabolome of
PR:PROJECT_SUMMARY               	Prochlorococcus with 25% of metabolites showing differential abundance. Infected
PR:PROJECT_SUMMARY               	cells exhibited decreased carbon fixation and the draw down of intracellular
PR:PROJECT_SUMMARY               	stores of carbon structures and energy such as glycogen and the osmolytes
PR:PROJECT_SUMMARY               	sucrose and aspartic acid. In contrast, another osmolyte, glucosylglycerol, was
PR:PROJECT_SUMMARY               	accumulated in high concentrations and came to dominate the virocell metabolome.
PR:PROJECT_SUMMARY               	Infected cells also experienced pseudocobalamin (pB12) stress, as evidenced by
PR:PROJECT_SUMMARY               	reduced pB12 concentrations, increased expression of genes to synthesize pB12,
PR:PROJECT_SUMMARY               	and depletion of metabolites whose synthesis relies on pB12 including
PR:PROJECT_SUMMARY               	S-adnosylmethionine (SAM) and the antioxidant glutathione. Collectively, our
PR:PROJECT_SUMMARY               	results suggest that the observed metabolic remodeling is driven by the demand
PR:PROJECT_SUMMARY               	for carbon and energy for virion production and infection-induced oxidative
PR:PROJECT_SUMMARY               	stress. Viral infection changes the substrate and vitamin pools provided by
PR:PROJECT_SUMMARY               	Prochlorococcus to the microbial community, potentially altering the speciation
PR:PROJECT_SUMMARY               	and flux of organic matter in marine systems and acting as a selective force on
PR:PROJECT_SUMMARY               	microbial community composition and function.
PR:INSTITUTE                     	University of Washington
PR:DEPARTMENT                    	School of Oceanography
PR:LABORATORY                    	Ingalls Lab
PR:LAST_NAME                     	Sacks
PR:FIRST_NAME                    	Joshua
PR:ADDRESS                       	Box 355351 School of Oceanography University of Washington, Seattle WA 98115
PR:EMAIL                         	jssacks@uw.edu
PR:PHONE                         	206 221 6750
PR:FUNDING_SOURCE                	Simons Foundation
PR:PUBLICATIONS                  	Sacks et al. in prep
#STUDY
ST:STUDY_TITLE                   	Remodeling of Prochlorococcus metabolism during viral infection
ST:STUDY_TYPE                    	Marine Microbial Metabolomics
ST:STUDY_SUMMARY                 	The marine cyanobacterium Prochlorococcus is the most abundant photoautotroph in
ST:STUDY_SUMMARY                 	the world and is a major contributor to oceanic primary productivity. Viruses
ST:STUDY_SUMMARY                 	are important controls on Prochlorococcus populations with up to 10% of
ST:STUDY_SUMMARY                 	Prochlorococcus cells infected. During infection, viruses remodel their host’s
ST:STUDY_SUMMARY                 	metabolic machinery, creating metabolically distinct cells, termed virocells.
ST:STUDY_SUMMARY                 	However, the specific consequences of viral infection on Prochlorococcus
ST:STUDY_SUMMARY                 	metabolism remain poorly understood. Here, we characterize the infection of
ST:STUDY_SUMMARY                 	non-axenic cultures of Prochlorococcus MED4 by the T7-like virus P-SSP7 using a
ST:STUDY_SUMMARY                 	combination of metabolomics, transcriptomics, and population modeling
ST:STUDY_SUMMARY                 	approaches. Three biological replicates (Replicates A, B, and C) of
ST:STUDY_SUMMARY                 	Prochlorococcus MED4 and associated heterotrophic bacteria were inoculated with
ST:STUDY_SUMMARY                 	3 different levels of the virus P-SSP7 at the beginning of the experiment. The
ST:STUDY_SUMMARY                 	treatments were as follows: Control (C), no virus added; Low Virus (LV), viruses
ST:STUDY_SUMMARY                 	added in a 1:0.001 host:virus ratio; and High Virus (HV), viruses add in a 1:0.7
ST:STUDY_SUMMARY                 	host:virus ratio. The experiment was run for 48 hours with metabolomics samples
ST:STUDY_SUMMARY                 	collected at timepoints of 0, 12, 24, 36, and 48 hours for the LV and HV
ST:STUDY_SUMMARY                 	treatments and 0, 6, 12, 18, 24, 30, 36, 42, 48 hours for the C treatment.
ST:STUDY_SUMMARY                 	P-SSP7 infection dramatically altered the metabolome of Prochlorococcus with 25%
ST:STUDY_SUMMARY                 	of metabolites showing differential abundance. Infected cells exhibited
ST:STUDY_SUMMARY                 	decreased carbon fixation and the draw down of intracellular stores of carbon
ST:STUDY_SUMMARY                 	structures and energy such as glycogen and the osmolytes sucrose and aspartic
ST:STUDY_SUMMARY                 	acid. In contrast, another osmolyte, glucosylglycerol, was accumulated in high
ST:STUDY_SUMMARY                 	concentrations and came to dominate the virocell metabolome. Infected cells also
ST:STUDY_SUMMARY                 	experienced pseudocobalamin (pB12) stress, as evidenced by reduced pB12
ST:STUDY_SUMMARY                 	concentrations, increased expression of genes to synthesize pB12, and depletion
ST:STUDY_SUMMARY                 	of metabolites whose synthesis relies on pB12 including S-adnosylmethionine
ST:STUDY_SUMMARY                 	(SAM) and the antioxidant glutathione. Collectively, our results suggest that
ST:STUDY_SUMMARY                 	the observed metabolic remodeling is driven by the demand for carbon and energy
ST:STUDY_SUMMARY                 	for virion production and infection-induced oxidative stress. Viral infection
ST:STUDY_SUMMARY                 	changes the substrate and vitamin pools provided by Prochlorococcus to the
ST:STUDY_SUMMARY                 	microbial community, potentially altering the speciation and flux of organic
ST:STUDY_SUMMARY                 	matter in marine systems and acting as a selective force on microbial community
ST:STUDY_SUMMARY                 	composition and function.
ST:INSTITUTE                     	University of Washington
ST:DEPARTMENT                    	School of Oceanography
ST:LABORATORY                    	Ingalls Lab
ST:LAST_NAME                     	Sacks
ST:FIRST_NAME                    	Joshua
ST:ADDRESS                       	Box 355351 School of Oceanography University of Washington, Seattle WA 98115
ST:EMAIL                         	jssacks@uw.edu
ST:PHONE                         	4074090052
#SUBJECT
SU:SUBJECT_TYPE                  	Bacteria
SU:SUBJECT_SPECIES               	Prochlorococcus marinus
SU:TAXONOMY_ID                   	59919
SU:GENOTYPE_STRAIN               	MED4
SU:GENDER                        	Not applicable
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data
SUBJECT_SAMPLE_FACTORS           	-	A_C_0	Sample source:Marine Microbe Culture | Treatment:C | Timepoint_h:0	Replicate=A; Volume_mL=50; RAW_FILE_NAME(HILIC_SampID)=200929_Smp_A_1C_T0; RAW_FILE_NAME(RP_SampID)=201006_Smp_A_1C_T0; RAW_FILE_NAME(Vitamin_SampID)=230511_Smp_A_1C_T0
SUBJECT_SAMPLE_FACTORS           	-	A_C_12	Sample source:Marine Microbe Culture | Treatment:C | Timepoint_h:12	Replicate=A; Volume_mL=50; RAW_FILE_NAME(HILIC_SampID)=200929_Smp_A_1C_T12; RAW_FILE_NAME(RP_SampID)=201006_Smp_A_1C_T12; RAW_FILE_NAME(Vitamin_SampID)=230511_Smp_A_1C_T12
SUBJECT_SAMPLE_FACTORS           	-	A_C_18	Sample source:Marine Microbe Culture | Treatment:C | Timepoint_h:18	Replicate=A; Volume_mL=50; RAW_FILE_NAME(HILIC_SampID)=200929_Smp_A_1C_T18; RAW_FILE_NAME(RP_SampID)=201006_Smp_A_1C_T18; RAW_FILE_NAME(Vitamin_SampID)=230511_Smp_A_1C_T18
SUBJECT_SAMPLE_FACTORS           	-	A_C_24	Sample source:Marine Microbe Culture | Treatment:C | Timepoint_h:24	Replicate=A; Volume_mL=100; RAW_FILE_NAME(HILIC_SampID)=200929_Smp_A_1C_T24; RAW_FILE_NAME(RP_SampID)=201006_Smp_A_1C_T24; RAW_FILE_NAME(Vitamin_SampID)=230511_Smp_A_1C_T24
SUBJECT_SAMPLE_FACTORS           	-	A_C_30	Sample source:Marine Microbe Culture | Treatment:C | Timepoint_h:30	Replicate=A; Volume_mL=50; RAW_FILE_NAME(HILIC_SampID)=200929_Smp_A_1C_T30; RAW_FILE_NAME(RP_SampID)=201006_Smp_A_1C_T30; RAW_FILE_NAME(Vitamin_SampID)=230511_Smp_A_1C_T30
SUBJECT_SAMPLE_FACTORS           	-	A_C_36	Sample source:Marine Microbe Culture | Treatment:C | Timepoint_h:36	Replicate=A; Volume_mL=300; RAW_FILE_NAME(HILIC_SampID)=200929_Smp_A_1C_T36; RAW_FILE_NAME(RP_SampID)=201006_Smp_A_1C_T36; RAW_FILE_NAME(Vitamin_SampID)=230511_Smp_A_1C_T36
SUBJECT_SAMPLE_FACTORS           	-	A_C_42	Sample source:Marine Microbe Culture | Treatment:C | Timepoint_h:42	Replicate=A; Volume_mL=50; RAW_FILE_NAME(HILIC_SampID)=200929_Smp_A_1C_T42; RAW_FILE_NAME(RP_SampID)=201006_Smp_A_1C_T42; RAW_FILE_NAME(Vitamin_SampID)=230511_Smp_A_1C_T42
SUBJECT_SAMPLE_FACTORS           	-	A_C_48	Sample source:Marine Microbe Culture | Treatment:C | Timepoint_h:48	Replicate=A; Volume_mL=100; RAW_FILE_NAME(HILIC_SampID)=200929_Smp_A_1C_T48; RAW_FILE_NAME(RP_SampID)=201006_Smp_A_1C_T48; RAW_FILE_NAME(Vitamin_SampID)=230511_Smp_A_1C_T48
SUBJECT_SAMPLE_FACTORS           	-	A_C_6	Sample source:Marine Microbe Culture | Treatment:C | Timepoint_h:6	Replicate=A; Volume_mL=50; RAW_FILE_NAME(HILIC_SampID)=200929_Smp_A_1C_T6; RAW_FILE_NAME(RP_SampID)=201006_Smp_A_1C_T6; RAW_FILE_NAME(Vitamin_SampID)=230511_Smp_A_1C_T6
SUBJECT_SAMPLE_FACTORS           	-	A_LV_0	Sample source:Marine Microbe Culture | Treatment:LV | Timepoint_h:0	Replicate=A; Volume_mL=50; RAW_FILE_NAME(HILIC_SampID)=200929_Smp_A_3LV_T0; RAW_FILE_NAME(RP_SampID)=201006_Smp_A_3LV_T0; RAW_FILE_NAME(Vitamin_SampID)=230511_Smp_A_3LV_T0
SUBJECT_SAMPLE_FACTORS           	-	A_LV_12	Sample source:Marine Microbe Culture | Treatment:LV | Timepoint_h:12	Replicate=A; Volume_mL=50; RAW_FILE_NAME(HILIC_SampID)=200929_Smp_A_3LV_T12; RAW_FILE_NAME(RP_SampID)=201006_Smp_A_3LV_T12; RAW_FILE_NAME(Vitamin_SampID)=230511_Smp_A_3LV_T12
SUBJECT_SAMPLE_FACTORS           	-	A_LV_24	Sample source:Marine Microbe Culture | Treatment:LV | Timepoint_h:24	Replicate=A; Volume_mL=100; RAW_FILE_NAME(HILIC_SampID)=200929_Smp_A_3LV_T24; RAW_FILE_NAME(RP_SampID)=201006_Smp_A_3LV_T24; RAW_FILE_NAME(Vitamin_SampID)=230511_Smp_A_3LV_T24
SUBJECT_SAMPLE_FACTORS           	-	A_LV_36	Sample source:Marine Microbe Culture | Treatment:LV | Timepoint_h:36	Replicate=A; Volume_mL=300; RAW_FILE_NAME(HILIC_SampID)=200929_Smp_A_3LV_T36; RAW_FILE_NAME(RP_SampID)=201006_Smp_A_3LV_T36; RAW_FILE_NAME(Vitamin_SampID)=230511_Smp_A_3LV_T36
SUBJECT_SAMPLE_FACTORS           	-	A_LV_48	Sample source:Marine Microbe Culture | Treatment:LV | Timepoint_h:48	Replicate=A; Volume_mL=100; RAW_FILE_NAME(HILIC_SampID)=200929_Smp_A_3LV_T48; RAW_FILE_NAME(RP_SampID)=201006_Smp_A_3LV_T48; RAW_FILE_NAME(Vitamin_SampID)=230511_Smp_A_3LV_T48
SUBJECT_SAMPLE_FACTORS           	-	A_HV_0	Sample source:Marine Microbe Culture | Treatment:HV | Timepoint_h:0	Replicate=A; Volume_mL=50; RAW_FILE_NAME(HILIC_SampID)=200929_Smp_A_6HV_T0; RAW_FILE_NAME(RP_SampID)=201006_Smp_A_6HV_T0; RAW_FILE_NAME(Vitamin_SampID)=230511_Smp_A_6HV_T0
SUBJECT_SAMPLE_FACTORS           	-	A_HV_12	Sample source:Marine Microbe Culture | Treatment:HV | Timepoint_h:12	Replicate=A; Volume_mL=50; RAW_FILE_NAME(HILIC_SampID)=200929_Smp_A_6HV_T12; RAW_FILE_NAME(RP_SampID)=201006_Smp_A_6HV_T12; RAW_FILE_NAME(Vitamin_SampID)=230511_Smp_A_6HV_T12
SUBJECT_SAMPLE_FACTORS           	-	A_HV_24	Sample source:Marine Microbe Culture | Treatment:HV | Timepoint_h:24	Replicate=A; Volume_mL=100; RAW_FILE_NAME(HILIC_SampID)=200929_Smp_A_6HV_T24; RAW_FILE_NAME(RP_SampID)=201006_Smp_A_6HV_T24; RAW_FILE_NAME(Vitamin_SampID)=230511_Smp_A_6HV_T24
SUBJECT_SAMPLE_FACTORS           	-	A_HV_36	Sample source:Marine Microbe Culture | Treatment:HV | Timepoint_h:36	Replicate=A; Volume_mL=300; RAW_FILE_NAME(HILIC_SampID)=200929_Smp_A_6HV_T36; RAW_FILE_NAME(RP_SampID)=201006_Smp_A_6HV_T36; RAW_FILE_NAME(Vitamin_SampID)=230511_Smp_A_6HV_T36
SUBJECT_SAMPLE_FACTORS           	-	A_HV_48	Sample source:Marine Microbe Culture | Treatment:HV | Timepoint_h:48	Replicate=A; Volume_mL=100; RAW_FILE_NAME(HILIC_SampID)=200929_Smp_A_6HV_T48; RAW_FILE_NAME(RP_SampID)=201006_Smp_A_6HV_T48; RAW_FILE_NAME(Vitamin_SampID)=230511_Smp_A_6HV_T48
SUBJECT_SAMPLE_FACTORS           	-	B_C_0	Sample source:Marine Microbe Culture | Treatment:C | Timepoint_h:0	Replicate=B; Volume_mL=50; RAW_FILE_NAME(HILIC_SampID)=200929_Smp_B_1C_T0; RAW_FILE_NAME(RP_SampID)=201006_Smp_B_1C_T0; RAW_FILE_NAME(Vitamin_SampID)=230511_Smp_B_1C_T0
SUBJECT_SAMPLE_FACTORS           	-	B_C_12	Sample source:Marine Microbe Culture | Treatment:C | Timepoint_h:12	Replicate=B; Volume_mL=50; RAW_FILE_NAME(HILIC_SampID)=200929_Smp_B_1C_T12; RAW_FILE_NAME(RP_SampID)=201006_Smp_B_1C_T12; RAW_FILE_NAME(Vitamin_SampID)=230511_Smp_B_1C_T12
SUBJECT_SAMPLE_FACTORS           	-	B_C_18	Sample source:Marine Microbe Culture | Treatment:C | Timepoint_h:18	Replicate=B; Volume_mL=50; RAW_FILE_NAME(HILIC_SampID)=200929_Smp_B_1C_T18; RAW_FILE_NAME(RP_SampID)=201006_Smp_B_1C_T18; RAW_FILE_NAME(Vitamin_SampID)=230511_Smp_B_1C_T18
SUBJECT_SAMPLE_FACTORS           	-	B_C_24	Sample source:Marine Microbe Culture | Treatment:C | Timepoint_h:24	Replicate=B; Volume_mL=100; RAW_FILE_NAME(HILIC_SampID)=200929_Smp_B_1C_T24; RAW_FILE_NAME(RP_SampID)=201006_Smp_B_1C_T24; RAW_FILE_NAME(Vitamin_SampID)=230511_Smp_B_1C_T24
SUBJECT_SAMPLE_FACTORS           	-	B_C_30	Sample source:Marine Microbe Culture | Treatment:C | Timepoint_h:30	Replicate=B; Volume_mL=50; RAW_FILE_NAME(HILIC_SampID)=200929_Smp_B_1C_T30; RAW_FILE_NAME(RP_SampID)=201006_Smp_B_1C_T30; RAW_FILE_NAME(Vitamin_SampID)=230511_Smp_B_1C_T30
SUBJECT_SAMPLE_FACTORS           	-	B_C_36	Sample source:Marine Microbe Culture | Treatment:C | Timepoint_h:36	Replicate=B; Volume_mL=300; RAW_FILE_NAME(HILIC_SampID)=200929_Smp_B_1C_T36; RAW_FILE_NAME(RP_SampID)=201006_Smp_B_1C_T36; RAW_FILE_NAME(Vitamin_SampID)=230511_Smp_B_1C_T36
SUBJECT_SAMPLE_FACTORS           	-	B_C_42	Sample source:Marine Microbe Culture | Treatment:C | Timepoint_h:42	Replicate=B; Volume_mL=50; RAW_FILE_NAME(HILIC_SampID)=200929_Smp_B_1C_T42; RAW_FILE_NAME(RP_SampID)=201006_Smp_B_1C_T42; RAW_FILE_NAME(Vitamin_SampID)=230511_Smp_B_1C_T42
SUBJECT_SAMPLE_FACTORS           	-	B_C_48	Sample source:Marine Microbe Culture | Treatment:C | Timepoint_h:48	Replicate=B; Volume_mL=100; RAW_FILE_NAME(HILIC_SampID)=200929_Smp_B_1C_T48; RAW_FILE_NAME(RP_SampID)=201006_Smp_B_1C_T48; RAW_FILE_NAME(Vitamin_SampID)=230511_Smp_B_1C_T48
SUBJECT_SAMPLE_FACTORS           	-	B_C_6	Sample source:Marine Microbe Culture | Treatment:C | Timepoint_h:6	Replicate=B; Volume_mL=50; RAW_FILE_NAME(HILIC_SampID)=200929_Smp_B_1C_T6; RAW_FILE_NAME(RP_SampID)=201006_Smp_B_1C_T6; RAW_FILE_NAME(Vitamin_SampID)=230511_Smp_B_1C_T6
SUBJECT_SAMPLE_FACTORS           	-	B_LV_0	Sample source:Marine Microbe Culture | Treatment:LV | Timepoint_h:0	Replicate=B; Volume_mL=50; RAW_FILE_NAME(HILIC_SampID)=200929_Smp_B_3LV_T0; RAW_FILE_NAME(RP_SampID)=201006_Smp_B_3LV_T0; RAW_FILE_NAME(Vitamin_SampID)=230511_Smp_B_3LV_T0
SUBJECT_SAMPLE_FACTORS           	-	B_LV_12	Sample source:Marine Microbe Culture | Treatment:LV | Timepoint_h:12	Replicate=B; Volume_mL=50; RAW_FILE_NAME(HILIC_SampID)=200929_Smp_B_3LV_T12; RAW_FILE_NAME(RP_SampID)=201006_Smp_B_3LV_T12; RAW_FILE_NAME(Vitamin_SampID)=230511_Smp_B_3LV_T12
SUBJECT_SAMPLE_FACTORS           	-	B_LV_24	Sample source:Marine Microbe Culture | Treatment:LV | Timepoint_h:24	Replicate=B; Volume_mL=100; RAW_FILE_NAME(HILIC_SampID)=200929_Smp_B_3LV_T24; RAW_FILE_NAME(RP_SampID)=201006_Smp_B_3LV_T24; RAW_FILE_NAME(Vitamin_SampID)=230511_Smp_B_3LV_T24
SUBJECT_SAMPLE_FACTORS           	-	B_LV_36	Sample source:Marine Microbe Culture | Treatment:LV | Timepoint_h:36	Replicate=B; Volume_mL=300; RAW_FILE_NAME(HILIC_SampID)=200929_Smp_B_3LV_T36; RAW_FILE_NAME(RP_SampID)=201006_Smp_B_3LV_T36; RAW_FILE_NAME(Vitamin_SampID)=230511_Smp_B_3LV_T36
SUBJECT_SAMPLE_FACTORS           	-	B_LV_48	Sample source:Marine Microbe Culture | Treatment:LV | Timepoint_h:48	Replicate=B; Volume_mL=100; RAW_FILE_NAME(HILIC_SampID)=200929_Smp_B_3LV_T48; RAW_FILE_NAME(RP_SampID)=201006_Smp_B_3LV_T48; RAW_FILE_NAME(Vitamin_SampID)=230511_Smp_B_3LV_T48
SUBJECT_SAMPLE_FACTORS           	-	B_HV_0	Sample source:Marine Microbe Culture | Treatment:HV | Timepoint_h:0	Replicate=B; Volume_mL=50; RAW_FILE_NAME(HILIC_SampID)=200929_Smp_B_6HV_T0; RAW_FILE_NAME(RP_SampID)=201006_Smp_B_6HV_T0; RAW_FILE_NAME(Vitamin_SampID)=230511_Smp_B_6HV_T0
SUBJECT_SAMPLE_FACTORS           	-	B_HV_12	Sample source:Marine Microbe Culture | Treatment:HV | Timepoint_h:12	Replicate=B; Volume_mL=50; RAW_FILE_NAME(HILIC_SampID)=200929_Smp_B_6HV_T12; RAW_FILE_NAME(RP_SampID)=201006_Smp_B_6HV_T12; RAW_FILE_NAME(Vitamin_SampID)=230511_Smp_B_6HV_T12
SUBJECT_SAMPLE_FACTORS           	-	B_HV_24	Sample source:Marine Microbe Culture | Treatment:HV | Timepoint_h:24	Replicate=B; Volume_mL=100; RAW_FILE_NAME(HILIC_SampID)=200929_Smp_B_6HV_T24; RAW_FILE_NAME(RP_SampID)=201006_Smp_B_6HV_T24; RAW_FILE_NAME(Vitamin_SampID)=230511_Smp_B_6HV_T24
SUBJECT_SAMPLE_FACTORS           	-	B_HV_36	Sample source:Marine Microbe Culture | Treatment:HV | Timepoint_h:36	Replicate=B; Volume_mL=270; RAW_FILE_NAME(HILIC_SampID)=200929_Smp_B_6HV_T36; RAW_FILE_NAME(RP_SampID)=201006_Smp_B_6HV_T36; RAW_FILE_NAME(Vitamin_SampID)=230511_Smp_B_6HV_T36
SUBJECT_SAMPLE_FACTORS           	-	B_HV_48	Sample source:Marine Microbe Culture | Treatment:HV | Timepoint_h:48	Replicate=B; Volume_mL=100; RAW_FILE_NAME(HILIC_SampID)=200929_Smp_B_6HV_T48; RAW_FILE_NAME(RP_SampID)=201006_Smp_B_6HV_T48; RAW_FILE_NAME(Vitamin_SampID)=230511_Smp_B_6HV_T48
SUBJECT_SAMPLE_FACTORS           	-	C_C_0	Sample source:Marine Microbe Culture | Treatment:C | Timepoint_h:0	Replicate=C; Volume_mL=50; RAW_FILE_NAME(HILIC_SampID)=200929_Smp_C_1C_T0; RAW_FILE_NAME(RP_SampID)=201006_Smp_C_1C_T0; RAW_FILE_NAME(Vitamin_SampID)=230511_Smp_C_1C_T0
SUBJECT_SAMPLE_FACTORS           	-	C_C_12	Sample source:Marine Microbe Culture | Treatment:C | Timepoint_h:12	Replicate=C; Volume_mL=50; RAW_FILE_NAME(HILIC_SampID)=200929_Smp_C_1C_T12; RAW_FILE_NAME(RP_SampID)=201006_Smp_C_1C_T12; RAW_FILE_NAME(Vitamin_SampID)=230511_Smp_C_1C_T12
SUBJECT_SAMPLE_FACTORS           	-	C_C_18	Sample source:Marine Microbe Culture | Treatment:C | Timepoint_h:18	Replicate=C; Volume_mL=50; RAW_FILE_NAME(HILIC_SampID)=200929_Smp_C_1C_T18; RAW_FILE_NAME(RP_SampID)=201006_Smp_C_1C_T18; RAW_FILE_NAME(Vitamin_SampID)=230511_Smp_C_1C_T18
SUBJECT_SAMPLE_FACTORS           	-	C_C_24	Sample source:Marine Microbe Culture | Treatment:C | Timepoint_h:24	Replicate=C; Volume_mL=100; RAW_FILE_NAME(HILIC_SampID)=200929_Smp_C_1C_T24; RAW_FILE_NAME(RP_SampID)=201006_Smp_C_1C_T24; RAW_FILE_NAME(Vitamin_SampID)=230511_Smp_C_1C_T24
SUBJECT_SAMPLE_FACTORS           	-	C_C_30	Sample source:Marine Microbe Culture | Treatment:C | Timepoint_h:30	Replicate=C; Volume_mL=50; RAW_FILE_NAME(HILIC_SampID)=200929_Smp_C_1C_T30; RAW_FILE_NAME(RP_SampID)=201006_Smp_C_1C_T30; RAW_FILE_NAME(Vitamin_SampID)=230511_Smp_C_1C_T30
SUBJECT_SAMPLE_FACTORS           	-	C_C_36	Sample source:Marine Microbe Culture | Treatment:C | Timepoint_h:36	Replicate=C; Volume_mL=100; RAW_FILE_NAME(HILIC_SampID)=200929_Smp_C_1C_T36; RAW_FILE_NAME(RP_SampID)=201006_Smp_C_1C_T36; RAW_FILE_NAME(Vitamin_SampID)=230511_Smp_C_1C_T36
SUBJECT_SAMPLE_FACTORS           	-	C_C_42	Sample source:Marine Microbe Culture | Treatment:C | Timepoint_h:42	Replicate=C; Volume_mL=50; RAW_FILE_NAME(HILIC_SampID)=200929_Smp_C_1C_T42; RAW_FILE_NAME(RP_SampID)=201006_Smp_C_1C_T42; RAW_FILE_NAME(Vitamin_SampID)=230511_Smp_C_1C_T42
SUBJECT_SAMPLE_FACTORS           	-	C_C_48	Sample source:Marine Microbe Culture | Treatment:C | Timepoint_h:48	Replicate=C; Volume_mL=100; RAW_FILE_NAME(HILIC_SampID)=200929_Smp_C_1C_T48; RAW_FILE_NAME(RP_SampID)=201006_Smp_C_1C_T48; RAW_FILE_NAME(Vitamin_SampID)=230511_Smp_C_1C_T48
SUBJECT_SAMPLE_FACTORS           	-	C_C_6	Sample source:Marine Microbe Culture | Treatment:C | Timepoint_h:6	Replicate=C; Volume_mL=50; RAW_FILE_NAME(HILIC_SampID)=200929_Smp_C_1C_T6; RAW_FILE_NAME(RP_SampID)=201006_Smp_C_1C_T6; RAW_FILE_NAME(Vitamin_SampID)=230511_Smp_C_1C_T6
SUBJECT_SAMPLE_FACTORS           	-	C_LV_0	Sample source:Marine Microbe Culture | Treatment:LV | Timepoint_h:0	Replicate=C; Volume_mL=50; RAW_FILE_NAME(HILIC_SampID)=200929_Smp_C_3LV_T0; RAW_FILE_NAME(RP_SampID)=201006_Smp_C_3LV_T0; RAW_FILE_NAME(Vitamin_SampID)=230511_Smp_C_3LV_T0
SUBJECT_SAMPLE_FACTORS           	-	C_LV_12	Sample source:Marine Microbe Culture | Treatment:LV | Timepoint_h:12	Replicate=C; Volume_mL=50; RAW_FILE_NAME(HILIC_SampID)=200929_Smp_C_3LV_T12; RAW_FILE_NAME(RP_SampID)=201006_Smp_C_3LV_T12; RAW_FILE_NAME(Vitamin_SampID)=230511_Smp_C_3LV_T12
SUBJECT_SAMPLE_FACTORS           	-	C_LV_24	Sample source:Marine Microbe Culture | Treatment:LV | Timepoint_h:24	Replicate=C; Volume_mL=100; RAW_FILE_NAME(HILIC_SampID)=200929_Smp_C_3LV_T24; RAW_FILE_NAME(RP_SampID)=201006_Smp_C_3LV_T24; RAW_FILE_NAME(Vitamin_SampID)=230511_Smp_C_3LV_T24
SUBJECT_SAMPLE_FACTORS           	-	C_LV_36	Sample source:Marine Microbe Culture | Treatment:LV | Timepoint_h:36	Replicate=C; Volume_mL=100; RAW_FILE_NAME(HILIC_SampID)=200929_Smp_C_3LV_T36; RAW_FILE_NAME(RP_SampID)=201006_Smp_C_3LV_T36; RAW_FILE_NAME(Vitamin_SampID)=230511_Smp_C_3LV_T36
SUBJECT_SAMPLE_FACTORS           	-	C_LV_48	Sample source:Marine Microbe Culture | Treatment:LV | Timepoint_h:48	Replicate=C; Volume_mL=100; RAW_FILE_NAME(HILIC_SampID)=200929_Smp_C_3LV_T48; RAW_FILE_NAME(RP_SampID)=201006_Smp_C_3LV_T48; RAW_FILE_NAME(Vitamin_SampID)=230511_Smp_C_3LV_T48
SUBJECT_SAMPLE_FACTORS           	-	C_HV_0	Sample source:Marine Microbe Culture | Treatment:HV | Timepoint_h:0	Replicate=C; Volume_mL=50; RAW_FILE_NAME(HILIC_SampID)=200929_Smp_C_6HV_T0; RAW_FILE_NAME(RP_SampID)=201006_Smp_C_6HV_T0; RAW_FILE_NAME(Vitamin_SampID)=230511_Smp_C_6HV_T0
SUBJECT_SAMPLE_FACTORS           	-	C_HV_12	Sample source:Marine Microbe Culture | Treatment:HV | Timepoint_h:12	Replicate=C; Volume_mL=50; RAW_FILE_NAME(HILIC_SampID)=200929_Smp_C_6HV_T12; RAW_FILE_NAME(RP_SampID)=201006_Smp_C_6HV_T12; RAW_FILE_NAME(Vitamin_SampID)=230511_Smp_C_6HV_T12
SUBJECT_SAMPLE_FACTORS           	-	C_HV_24	Sample source:Marine Microbe Culture | Treatment:HV | Timepoint_h:24	Replicate=C; Volume_mL=100; RAW_FILE_NAME(HILIC_SampID)=200929_Smp_C_6HV_T24; RAW_FILE_NAME(RP_SampID)=201006_Smp_C_6HV_T24; RAW_FILE_NAME(Vitamin_SampID)=230511_Smp_C_6HV_T24
SUBJECT_SAMPLE_FACTORS           	-	C_HV_36	Sample source:Marine Microbe Culture | Treatment:HV | Timepoint_h:36	Replicate=C; Volume_mL=100; RAW_FILE_NAME(HILIC_SampID)=200929_Smp_C_6HV_T36; RAW_FILE_NAME(RP_SampID)=201006_Smp_C_6HV_T36; RAW_FILE_NAME(Vitamin_SampID)=230511_Smp_C_6HV_T36
SUBJECT_SAMPLE_FACTORS           	-	C_HV_48	Sample source:Marine Microbe Culture | Treatment:HV | Timepoint_h:48	Replicate=C; Volume_mL=100; RAW_FILE_NAME(HILIC_SampID)=200929_Smp_C_6HV_T48; RAW_FILE_NAME(RP_SampID)=201006_Smp_C_6HV_T48; RAW_FILE_NAME(Vitamin_SampID)=230511_Smp_C_6HV_T48
#COLLECTION
CO:COLLECTION_SUMMARY            	Particulate metabolites were sampled using gentle vacuum filtration onto 47 mm
CO:COLLECTION_SUMMARY            	0.2-mm polytetrafluoroethylene (PTFE) omnipore filters (Omnipore Membrane
CO:COLLECTION_SUMMARY            	Filters, Merck Millipore Ltd). Glass and polysulfone filtration rigs were used.
CO:COLLECTION_SUMMARY            	Glass rigs were combusted at 450 ˚C for 4 hours before and between experiments.
CO:COLLECTION_SUMMARY            	The polysulfone filtration setups were soaked in 10% HCl for 24 hours and triple
CO:COLLECTION_SUMMARY            	rinsed with MiliQ water before and between experiments. In between sampling
CO:COLLECTION_SUMMARY            	different treatments, timepoints, and biological replicates, the filtration
CO:COLLECTION_SUMMARY            	setups were triple rinsed with MiliQ water, rinsed with 10% HCl, and then triple
CO:COLLECTION_SUMMARY            	rinsed again with MiliQ water. After filtration, samples were wrapped in
CO:COLLECTION_SUMMARY            	combusted foil and flash frozen in liquid nitrogen before storage at -80˚C
CO:COLLECTION_SUMMARY            	until analysis. Sample volumes ranged from 50–300 mL to account for variations
CO:COLLECTION_SUMMARY            	in biomass throughout the experiment and filtration times ranged from 5–15
CO:COLLECTION_SUMMARY            	minutes.
CO:SAMPLE_TYPE                   	Bacterial cells
CO:STORAGE_CONDITIONS            	-80℃
#TREATMENT
TR:TREATMENT_SUMMARY             	Three biological replicates (Replicates A, B, and C) of Prochlorococcus MED4 and
TR:TREATMENT_SUMMARY             	associated heterotrophic bacteria were inoculated with 3 different levels of the
TR:TREATMENT_SUMMARY             	virus P-SSP7 at the beginning of the experiment. The treatments were as follows:
TR:TREATMENT_SUMMARY             	Control (C), no virus added; Low Virus (LV), viruses added in a 1:0.001
TR:TREATMENT_SUMMARY             	host:virus ratio; and High Virus (HV), viruses add in a 1:0.7 host:virus ratio.
TR:TREATMENT_SUMMARY             	The experiment was run for 48 hours with samples collected at timepoints
TR:TREATMENT_SUMMARY             	(timepoint_h) of 0, 12, 24, 36, and 48 hours for the LV and HV treatments and 0,
TR:TREATMENT_SUMMARY             	6, 12, 18, 24, 30, 36, 42, 48 hours for the C treatment.
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	Each sample was extracted using a modified Bligh-Dyer extraction. Briefly,
SP:SAMPLEPREP_SUMMARY            	filters were cut up and put into 15 mL teflon centrifuge tubes containing a
SP:SAMPLEPREP_SUMMARY            	mixture of 100 µm and 400 µm silica beads. Heavy isotope-labeled internal
SP:SAMPLEPREP_SUMMARY            	standards were added along with ~2 mL of cold aqueous solvent (50:50
SP:SAMPLEPREP_SUMMARY            	methanol:water) and ~3 mL of cold organic solvent (dichloromethane). The samples
SP:SAMPLEPREP_SUMMARY            	were shaken on a FastPrep-24 Homogenizer for 30 seconds and chilled in a -20 °C
SP:SAMPLEPREP_SUMMARY            	freezer repeatedly for three cycles of bead-beating and a total of 30 minutes of
SP:SAMPLEPREP_SUMMARY            	chilling. The organic and aqueous layers were separated by spinning samples in a
SP:SAMPLEPREP_SUMMARY            	centrifuge at 4,300 rpm for 2 minutes at 4 °C. The aqueous layer was removed to
SP:SAMPLEPREP_SUMMARY            	a new glass centrifuge tube. The remaining organic fraction was rinsed three
SP:SAMPLEPREP_SUMMARY            	more times with additions of 1 to 2 mL of 50:50 methanol:water. All aqueous
SP:SAMPLEPREP_SUMMARY            	rinses were combined for each sample and dried down under N2 gas. The remaining
SP:SAMPLEPREP_SUMMARY            	organic layer was transferred into a clean glass centrifuge tube and the
SP:SAMPLEPREP_SUMMARY            	remaining bead beating tube was rinsed two more times with cold organic solvent.
SP:SAMPLEPREP_SUMMARY            	The combined organic rinses were centrifuged, transferred to a new tube, and
SP:SAMPLEPREP_SUMMARY            	dried under N2 gas. Dried aqueous fractions were re-dissolved in 380 µL of
SP:SAMPLEPREP_SUMMARY            	water. Dried organic fractions were re-dissolved in 380 µL of 1:1
SP:SAMPLEPREP_SUMMARY            	water:acetonitrile. 20 µL of isotope-labeled injection standards in water were
SP:SAMPLEPREP_SUMMARY            	added to both fractions. Blank filters were extracted alongside samples as
SP:SAMPLEPREP_SUMMARY            	methodological blanks.
SP:PROCESSING_STORAGE_CONDITIONS 	On ice
SP:EXTRACT_STORAGE               	-80℃
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_TYPE           	Reversed phase
CH:INSTRUMENT_NAME               	Waters Acquity
CH:COLUMN_NAME                   	Waters ACQUITY UPLC HSS CN (100 x 2.1mm,1.8um)
CH:SOLVENT_A                     	100% water; 0.1% formic acid; 20mM ammonium formate
CH:SOLVENT_B                     	100% acetonitrile
CH:FLOW_GRADIENT                 	The column was held at 2% B for 0.5 minutes, ramped to 25% B for 7.8 minutes,
CH:FLOW_GRADIENT                 	ramped to 95% B, held at 95% B for 1 minute, and equilibrated back to 2% B for
CH:FLOW_GRADIENT                 	1.7 minutes (total run time is 11 minutes)
CH:FLOW_RATE                     	0.6 mL/min
CH:COLUMN_TEMPERATURE            	35C
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
#MS
MS:INSTRUMENT_NAME               	Waters Xevo-TQ-S
MS:INSTRUMENT_TYPE               	Triple quadrupole
MS:MS_TYPE                       	ESI
MS:ION_MODE                      	POSITIVE
MS:MS_COMMENTS                   	MS acquisition Comments: The selected reaction monitoring (SRM) transitions were
MS:MS_COMMENTS                   	monitored over a 5 to 10 minute window around the retention time. For most
MS:MS_COMMENTS                   	metabolites, two SRM transitions were monitored, one for quantification and an
MS:MS_COMMENTS                   	additional for compound confirmation. Scheduling was set up to ensure at least
MS:MS_COMMENTS                   	12 data points per peak were collected. Data processing Comments: Data was
MS:MS_COMMENTS                   	processed as in Heal et al. 2014 (DOI: 10.1021/acs.analchem.7b04400).
MS:MS_COMMENTS                   	Software/procedures used for feature assignments: Peaks were integrated using
MS:MS_COMMENTS                   	Skyline. Data was processed using quality control, and best-matched internal
MS:MS_COMMENTS                   	standard normalization. MS parameters were as follows: capillary voltage of 0.5
MS:MS_COMMENTS                   	kV, source temperature of 130C, cone gas flow at 150 L/h and desolvation gas
MS:MS_COMMENTS                   	flow at 1000 L/h, Desolvation temperature was 500C
#MS_METABOLITE_DATA
MS_METABOLITE_DATA:UNITS	nmol/L
MS_METABOLITE_DATA_START
Samples	A_C_0	A_C_12	A_C_18	A_C_24	A_C_30	A_C_36	A_C_42	A_C_48	A_C_6	A_LV_0	A_LV_12	A_LV_24	A_LV_36	A_LV_48	A_HV_0	A_HV_12	A_HV_24	A_HV_36	A_HV_48	B_C_0	B_C_12	B_C_18	B_C_24	B_C_30	B_C_36	B_C_42	B_C_48	B_C_6	B_LV_0	B_LV_12	B_LV_24	B_LV_36	B_LV_48	B_HV_0	B_HV_12	B_HV_24	B_HV_36	B_HV_48	C_C_0	C_C_12	C_C_18	C_C_24	C_C_30	C_C_36	C_C_42	C_C_48	C_C_6	C_LV_0	C_LV_12	C_LV_24	C_LV_36	C_LV_48	C_HV_0	C_HV_12	C_HV_24	C_HV_36	C_HV_48
Factors	Sample source:Marine Microbe Culture | Treatment:C | Timepoint_h:0	Sample source:Marine Microbe Culture | Treatment:C | Timepoint_h:12	Sample source:Marine Microbe Culture | Treatment:C | Timepoint_h:18	Sample source:Marine Microbe Culture | Treatment:C | Timepoint_h:24	Sample source:Marine Microbe Culture | Treatment:C | Timepoint_h:30	Sample source:Marine Microbe Culture | Treatment:C | Timepoint_h:36	Sample source:Marine Microbe Culture | Treatment:C | Timepoint_h:42	Sample source:Marine Microbe Culture | Treatment:C | Timepoint_h:48	Sample source:Marine Microbe Culture | Treatment:C | Timepoint_h:6	Sample source:Marine Microbe Culture | Treatment:LV | Timepoint_h:0	Sample source:Marine Microbe Culture | Treatment:LV | Timepoint_h:12	Sample source:Marine Microbe Culture | Treatment:LV | Timepoint_h:24	Sample source:Marine Microbe Culture | Treatment:LV | Timepoint_h:36	Sample source:Marine Microbe Culture | Treatment:LV | Timepoint_h:48	Sample source:Marine Microbe Culture | Treatment:HV | Timepoint_h:0	Sample source:Marine Microbe Culture | Treatment:HV | Timepoint_h:12	Sample source:Marine Microbe Culture | Treatment:HV | Timepoint_h:24	Sample source:Marine Microbe Culture | Treatment:HV | Timepoint_h:36	Sample source:Marine Microbe Culture | Treatment:HV | Timepoint_h:48	Sample source:Marine Microbe Culture | Treatment:C | Timepoint_h:0	Sample source:Marine Microbe Culture | Treatment:C | Timepoint_h:12	Sample source:Marine Microbe Culture | Treatment:C | Timepoint_h:18	Sample source:Marine Microbe Culture | Treatment:C | Timepoint_h:24	Sample source:Marine Microbe Culture | Treatment:C | Timepoint_h:30	Sample source:Marine Microbe Culture | Treatment:C | Timepoint_h:36	Sample source:Marine Microbe Culture | Treatment:C | Timepoint_h:42	Sample source:Marine Microbe Culture | Treatment:C | Timepoint_h:48	Sample source:Marine Microbe Culture | Treatment:C | Timepoint_h:6	Sample source:Marine Microbe Culture | Treatment:LV | Timepoint_h:0	Sample source:Marine Microbe Culture | Treatment:LV | Timepoint_h:12	Sample source:Marine Microbe Culture | Treatment:LV | Timepoint_h:24	Sample source:Marine Microbe Culture | Treatment:LV | Timepoint_h:36	Sample source:Marine Microbe Culture | Treatment:LV | Timepoint_h:48	Sample source:Marine Microbe Culture | Treatment:HV | Timepoint_h:0	Sample source:Marine Microbe Culture | Treatment:HV | Timepoint_h:12	Sample source:Marine Microbe Culture | Treatment:HV | Timepoint_h:24	Sample source:Marine Microbe Culture | Treatment:HV | Timepoint_h:36	Sample source:Marine Microbe Culture | Treatment:HV | Timepoint_h:48	Sample source:Marine Microbe Culture | Treatment:C | Timepoint_h:0	Sample source:Marine Microbe Culture | Treatment:C | Timepoint_h:12	Sample source:Marine Microbe Culture | Treatment:C | Timepoint_h:18	Sample source:Marine Microbe Culture | Treatment:C | Timepoint_h:24	Sample source:Marine Microbe Culture | Treatment:C | Timepoint_h:30	Sample source:Marine Microbe Culture | Treatment:C | Timepoint_h:36	Sample source:Marine Microbe Culture | Treatment:C | Timepoint_h:42	Sample source:Marine Microbe Culture | Treatment:C | Timepoint_h:48	Sample source:Marine Microbe Culture | Treatment:C | Timepoint_h:6	Sample source:Marine Microbe Culture | Treatment:LV | Timepoint_h:0	Sample source:Marine Microbe Culture | Treatment:LV | Timepoint_h:12	Sample source:Marine Microbe Culture | Treatment:LV | Timepoint_h:24	Sample source:Marine Microbe Culture | Treatment:LV | Timepoint_h:36	Sample source:Marine Microbe Culture | Treatment:LV | Timepoint_h:48	Sample source:Marine Microbe Culture | Treatment:HV | Timepoint_h:0	Sample source:Marine Microbe Culture | Treatment:HV | Timepoint_h:12	Sample source:Marine Microbe Culture | Treatment:HV | Timepoint_h:24	Sample source:Marine Microbe Culture | Treatment:HV | Timepoint_h:36	Sample source:Marine Microbe Culture | Treatment:HV | Timepoint_h:48
Hydroxocobalamin	5.566733888544325e-4	6.665866535717929e-4	6.347753385119344e-4	0.00116737903697303	3.652145058610861e-4	4.075304624328113e-4	0.00144440115163059	7.86745684822366e-4	3.26875752924827e-4	5.842227378023057e-4	4.90046546177556e-4	0.00116162064720395	0.00146292460345686	0.00166241681901951	6.20739263449796e-4	7.655173051703574e-4	0.00186068537675623	3.6274761673523034e-4	0.00339090737222493	4.6250098564400464e-4	0.00105772149022239	3.304861117734688e-4	0.00113934287678481	0.00109605266633	0.0013496604862719	0.00132144816481772	0.00135486955596433	8.56939319583184e-5	5.353294625656099e-4	0.0014445413229605	9.905207027757694e-4	0.00125736966217462	0.00108743402374799	6.047218476973748e-4	0.00119388846618958	0.00143735091257779	1.0740645692948448e-4	0.00568576397232701	4.417480519893773e-4	8.968502644629755e-4	8.46649986285271e-4	9.299894187426124e-4	0.0011082021110599	5.572586988671451e-4	3.49000092563933e-4	0.00122728144422877	5.155615166346415e-4	4.211883274651601e-4	8.656413072802775e-4	0.00103245087248906	4.748436395043141e-4	0.00288117433102432	4.0662187439797473e-4	6.708448312694565e-4	4.980401003964797e-4	4.4349261678455e-4	0.0020875356263435
Cyanopseudocobalamin	9.936365136590146e-4	0.00158879759391795	0.0012402957243148	0.0027942380220235	4.453374645978501e-4	7.804531020010258e-4	0.00388915006799851	0.00221378902406221	6.753098682440512e-4	0.00105607153559935	7.507121639719986e-4	0.00108168420049189	0.00183920358712335	9.998800158530484e-5	8.969593053811843e-4	5.618743234715965e-4	1.4520485462720303e-4	1.358489682925263e-4	2.809575987315234e-4	0.0013491993652852	0.00160462604710708	5.655478734531926e-4	0.00146142401806094	0.00228643998924066	7.760915064966828e-4	4.064254247371611e-4	0.00319062888185961	3.896057772553613e-4	0.00142538848534857	4.204452038046153e-4	0.00175055641508252	0.00154107295119228	4.098665038923836e-4	2.6110816663346985e-4	7.407082545645368e-4	3.177927289016438e-4	6.707686921013457e-5	1.0251197055736108e-4	0.00113846839311423	0.00131756289590266	0.00192294247402418	0.00246450242374497	0.00216530991557768	0.00128330793644284	8.66538837244296e-4	0.00222076059422895	6.795965280974019e-4	0.00155862747897869	0.00139623511094664	0.00237084733621	0.00135392031129771	4.597838659510666e-4	0.00100141279053243	0.00122147325125193	3.18559116241337e-4	2.174317521443549e-4	2.0180766891247644e-4
Hyrodoxopseudocobalamin	0.00156053385614	9.9973564177203e-4	0.00119359353813463	0.00136676797089023	0.00142766346360525	9.69012390875502e-4	0.00170348739752319	0.00141386496947626	0.00105920020304253	0.00161317055827838	7.702979999133503e-4	0.00159777297446418	9.98605068442998e-4	9.156533486213694e-4	0.00201430728229023	8.277827358593021e-4	4.333342679118121e-4	8.869130958463899e-5	1.2772411580970513e-4	8.251175863839407e-4	0.00143030957629864	9.985363245081174e-4	0.00212620548669148	0.0018440232675966	0.00228013373287858	0.00226215349231088	0.00280985122788865	0.00102007961610062	0.00151721248597739	0.00265103687433005	0.00159584074109455	5.981272286070014e-4	2.1045479744071817e-4	0.00246540921337112	0.00118160671108949	3.135802181522356e-4	2.101331698342779e-5	1.5619996883514488e-4	0.00153669980511748	0.00164218895958988	0.00149601820205428	0.00175291957469716	0.00248100033780199	0.00147189542650526	0.00105378106577596	0.00242760850997891	0.00108852953845949	0.00104765122198263	0.00221022115110526	0.0012103903254234	5.27594893820758e-4	1.298403714050219e-4	6.397500947785597e-4	0.00101828381300174	2.2828591654968435e-4	5.956926357132388e-5	9.544407918788629e-5
MS_METABOLITE_DATA_END
#METABOLITES
METABOLITES_START
metabolite_name	mz	RT_minute	PubChem_Code	KEGG_Code
Hydroxocobalamin	664.7861	4.35	70678542	cpd:C08230
Cyanopseudocobalamin	673.2805	4.3	NA	NA
Hyrodoxopseudocobalamin	659.775	3.5	NA	NA
METABOLITES_END
#END