#METABOLOMICS WORKBENCH msekiya_20250728_212836 DATATRACK_ID:6225 STUDY_ID:ST004082 ANALYSIS_ID:AN006759 PROJECT_ID:PR002563
VERSION             	1
CREATED_ON             	July 30, 2025, 7:45 am
#PROJECT
PR:PROJECT_TITLE                 	IMR90 cells treated with exosomes with or without CtBP2
PR:PROJECT_SUMMARY               	We prepared two types of MEF (mouse embryonic fibroblast) cells: one is cells
PR:PROJECT_SUMMARY               	with wild-type CtBP2-HA knock-in and the other is cells with CtBP2 mutant-HA
PR:PROJECT_SUMMARY               	knock-in. The latter does not secrete CtBP2 in exosomes. We isolated exosomes
PR:PROJECT_SUMMARY               	from these cells and treated IMR90 fibroblasts (WT and Mut, respectively).
PR:INSTITUTE                     	University of Tsukuba
PR:LAST_NAME                     	Sekiya
PR:FIRST_NAME                    	Motohiro
PR:ADDRESS                       	1-1-1 Tennodai, Tsukuba, Ibaraki, 3058575, Japan
PR:EMAIL                         	msekiya@md.tsukuba.ac.jp
PR:PHONE                         	+81-29-853-3053
#STUDY
ST:STUDY_TITLE                   	The secreted metabolite sensor CtBP2 links metabolism to healthy lifespan
ST:STUDY_SUMMARY                 	While metabolic homeostasis is coordinated by metabolite-sensing molecules
ST:STUDY_SUMMARY                 	within a cell, their roles in horizontal regulation across cells remain
ST:STUDY_SUMMARY                 	underexplored. Here, we describe a system regulated by a metabolite sensor
ST:STUDY_SUMMARY                 	CtBP2. CtBP2 is secreted via exosomes in response to reductive metabolism, which
ST:STUDY_SUMMARY                 	is suppressed by oxidative stress. A series of pro-longevity agents induce CtBP2
ST:STUDY_SUMMARY                 	secretion, and administration of exosomal CtBP2 extends lifespan of aged mice.
ST:STUDY_SUMMARY                 	Consistently, serum CtBP2 concentrations are higher in human subjects from
ST:STUDY_SUMMARY                 	families with a history of longevity and exhibit an age-dependent decline.
ST:STUDY_SUMMARY                 	Healthspan is also improved by exosomal CtBP2, as exemplified by protection
ST:STUDY_SUMMARY                 	against frailty in mice and negative associations between serum CtBP2 levels and
ST:STUDY_SUMMARY                 	cardiovascular diseases in humans. These health benefits could be attributed to
ST:STUDY_SUMMARY                 	the proper channeling of reducing equivalents into a chain of activation of
ST:STUDY_SUMMARY                 	CYB5R3 and AMPK. Our findings illustrate a previously uncharacterized metabolic
ST:STUDY_SUMMARY                 	system that provides insights into the regulation of human health and holds
ST:STUDY_SUMMARY                 	promise for future clinical applications.
ST:INSTITUTE                     	University of Tsukuba
ST:LAST_NAME                     	Sekiya
ST:FIRST_NAME                    	Motohiro
ST:ADDRESS                       	1-1-1 Tennodai, Tsukuba, Ibaraki, 3058575, Japan
ST:EMAIL                         	msekiya@md.tsukuba.ac.jp
ST:PHONE                         	+81298533053
#SUBJECT
SU:SUBJECT_TYPE                  	Cultured cells
SU:SUBJECT_SPECIES               	Homo sapiens
SU:TAXONOMY_ID                   	9606
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data
SUBJECT_SAMPLE_FACTORS           	-	WT-1	Group:WT | Sample source:IMR90 fibroblasts	RAW_FILE_NAME(Raw data(positive))=TSUUY09101_C_20240802_004_d1.mzML; RAW_FILE_NAME(Raw data(negative))=TSUUY09101_A_20240802_004_d2.mzML
SUBJECT_SAMPLE_FACTORS           	-	WT-2	Group:WT | Sample source:IMR90 fibroblasts	RAW_FILE_NAME(Raw data(positive))=TSUUY09101_C_20240802_005_d1.mzML; RAW_FILE_NAME(Raw data(negative))=TSUUY09101_A_20240802_005_d2.mzML
SUBJECT_SAMPLE_FACTORS           	-	WT-3	Group:WT | Sample source:IMR90 fibroblasts	RAW_FILE_NAME(Raw data(positive))=TSUUY09101_C_20240802_006_d1.mzML; RAW_FILE_NAME(Raw data(negative))=TSUUY09101_A_20240802_006_d2.mzML
SUBJECT_SAMPLE_FACTORS           	-	Mut-1	Group:Mut | Sample source:IMR90 fibroblasts	RAW_FILE_NAME(Raw data(positive))=TSUUY09101_C_20240802_007_d1.mzML; RAW_FILE_NAME(Raw data(negative))=TSUUY09101_A_20240802_007_d2.mzML
SUBJECT_SAMPLE_FACTORS           	-	Mut-2	Group:Mut | Sample source:IMR90 fibroblasts	RAW_FILE_NAME(Raw data(positive))=TSUUY09101_C_20240802_008_d1.mzML; RAW_FILE_NAME(Raw data(negative))=TSUUY09101_A_20240802_008_d2.mzML
SUBJECT_SAMPLE_FACTORS           	-	Mut-3	Group:Mut | Sample source:IMR90 fibroblasts	RAW_FILE_NAME(Raw data(positive))=TSUUY09101_C_20240802_009_d1.mzML; RAW_FILE_NAME(Raw data(negative))=TSUUY09101_A_20240802_009_d2.mzML
#COLLECTION
CO:COLLECTION_SUMMARY            	We identified an anti-aging effect of CtBP2 in exosomes. To investigate the
CO:COLLECTION_SUMMARY            	mechanistic insights behind this, we decided to treat IMR90 fibroblasts with
CO:COLLECTION_SUMMARY            	exosomes with or without CtBP2. Since we revealed that CtBP2 mutant lacking the
CO:COLLECTION_SUMMARY            	metabolite sensing pocket cannot be secreted via exosomes, we prepared two types
CO:COLLECTION_SUMMARY            	of MEF cells: one is cells with wild-type CtBP2-HA knock-in and the other is
CO:COLLECTION_SUMMARY            	cells with the CtBP2 mutant-HA knock-in. We isolated exosomes from these cells
CO:COLLECTION_SUMMARY            	and treated IMR90 fibroblasts for 1.5 days. Exosomes from MEF cells with
CO:COLLECTION_SUMMARY            	wild-type CtBP2-HA knock-in contain CtBP2, and exosomes from those with mutant
CO:COLLECTION_SUMMARY            	CtBP2-HA knock-in does not. WT and Mut denote metabolites extracted from IMR90
CO:COLLECTION_SUMMARY            	cells treated with exosomes with and without CtBP2, respectively.
CO:SAMPLE_TYPE                   	Fibroblast cells
#TREATMENT
TR:TREATMENT_SUMMARY             	IMR90 fibroblast cells were treated with exosomes with or without CtBP2, for 1.5
TR:TREATMENT_SUMMARY             	days in DMEM 10% exosome-free fetal bovine serum.
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	Culture medium was aspirated from the dish, and the cells were washed twice with
SP:SAMPLEPREP_SUMMARY            	5% mannitol solution (10 mL and 2 mL for the first and second wash,
SP:SAMPLEPREP_SUMMARY            	respectively). The cells were then treated with 800 µL of methanol and
SP:SAMPLEPREP_SUMMARY            	incubated at room temperature for 30 sec to suppress enzyme activity. Next, 550
SP:SAMPLEPREP_SUMMARY            	µL of Milli-Q water containing internal standards (H3304-1002, Human Metabolome
SP:SAMPLEPREP_SUMMARY            	Technologies, Inc. (HMT), Tsuruoka, Yamagata, Japan) was added to the cell
SP:SAMPLEPREP_SUMMARY            	extract, followed by further incubation at room temperature for 30 sec. The cell
SP:SAMPLEPREP_SUMMARY            	extract was then centrifuged at 2,300 ×g, 4ºC for 5 min, after which 700 µL
SP:SAMPLEPREP_SUMMARY            	of the supernatant was centrifugally filtered through a Millipore 5-kDa cutoff
SP:SAMPLEPREP_SUMMARY            	filter (UltrafreeMC-PLHCC, HMT) at 9,100 ×g, 4ºC for 120 min to remove
SP:SAMPLEPREP_SUMMARY            	macromolecules. Subsequently, the filtrate was evaporated to dryness under
SP:SAMPLEPREP_SUMMARY            	vacuum and reconstituted in 50 µL of Milli-Q water for metabolome analysis at
SP:SAMPLEPREP_SUMMARY            	HMT.
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_TYPE           	CE
CH:INSTRUMENT_NAME               	Agilent 7100 CE
CH:COLUMN_NAME                   	Fused silica capillary (80 cm x 50 um)
CH:SOLVENT_A                     	Cation Buffer Solution (p/n:H3301-1001)
CH:SOLVENT_B                     	N/A
CH:FLOW_GRADIENT                 	N/A
CH:FLOW_RATE                     	N/A
CH:COLUMN_TEMPERATURE            	N/A
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
#MS
MS:INSTRUMENT_NAME               	Thermo Q Exactive Plus Orbitrap
MS:INSTRUMENT_TYPE               	Orbitrap
MS:MS_TYPE                       	ESI
MS:ION_MODE                      	POSITIVE
MS:MS_COMMENTS                   	CE-HRMS(omega) Positive (Cation) mode
#MS_METABOLITE_DATA
MS_METABOLITE_DATA:UNITS	arbitrarry unit
MS_METABOLITE_DATA_START
Samples	WT-1	WT-2	WT-3	Mut-1	Mut-2	Mut-3									
Factors	Group:WT | Sample source:IMR90 fibroblasts	Group:WT | Sample source:IMR90 fibroblasts	Group:WT | Sample source:IMR90 fibroblasts	Group:Mut | Sample source:IMR90 fibroblasts	Group:Mut | Sample source:IMR90 fibroblasts	Group:Mut | Sample source:IMR90 fibroblasts
Urea	5.1E-02	1.1E-01	1.4E-01	3.7E-02	8.9E-02	7.3E-02									
Ethanolamine	2.1E-02	2.0E-02	2.1E-02	2.6E-02	2.6E-02	2.3E-02									
Aminoacetone	N.D.	N.D.	5.4E-03	N.D.	N.D.	5.0E-03									
Methylguanidine	N.D.	2.1E-04	3.6E-04	6.9E-05	1.1E-04	1.4E-04									
Isobutylamine	N.D.	1.3E-02	1.1E-02	N.D.	N.D.	N.D.									
Gly	6.2E+00	5.9E+00	6.0E+00	6.1E+00	6.1E+00	5.4E+00									
Trimethylamine N-oxide	1.1E-03	1.3E-03	1.1E-03	1.1E-03	1.2E-03	7.2E-04									
Isopropanolamine	1.1E-03	1.1E-03	8.4E-04	1.4E-03	1.3E-03	1.1E-03									
Piperidine	6.7E-04	8.1E-04	8.7E-04	8.1E-04	7.9E-04	8.1E-04									
Morpholine	8.5E-03	2.3E-02	2.1E-02	1.1E-02	9.3E-03	9.9E-03									
Putrescine	4.5E-02	4.6E-02	3.8E-02	3.6E-02	3.5E-02	3.2E-02									
Ala	7.4E+00	7.2E+00	7.3E+00	7.3E+00	6.5E+00	6.5E+00									
β-Ala	2.7E-01	2.7E-01	2.8E-01	2.9E-01	2.8E-01	2.8E-01									
Sarcosine	4.3E-02	4.0E-02	4.1E-02	4.5E-02	3.6E-02	4.1E-02									
2-Amino-2-methyl-1-propanol	2.7E-03	2.2E-03	2.8E-03	2.8E-03	2.8E-03	2.0E-03									
Glycerol	6.8E-01	7.8E-01	8.5E-01	5.4E-01	5.4E-01	6.5E-01									
Imidazole-4-methanol	N.D.	N.D.	N.D.	N.D.	1.6E-04	N.D.									
1-Methyl-2-pyrrolidone	N.D.	1.4E-02	9.4E-03	6.5E-03	1.3E-02	7.2E-03									
Cyclohexylamine	1.5E-03	3.4E-03	2.6E-03	1.8E-03	1.6E-03	1.5E-03									
Azetidine 2-carboxylic acid	3.7E-03	6.4E-04	3.3E-03	N.D.	5.4E-03	3.6E-03									
Homoserinelactone	1.7E-02	7.3E-03	5.3E-03	1.1E-02	1.2E-02	1.0E-02									
Cadaverine-1"	N.D.	N.D.	N.D.	N.D.	1.0E-04	N.D.									
Cadaverine-2"	1.3E-03	1.5E-03	1.3E-03	8.7E-04	1.1E-03	8.9E-04									
"N,N-Dimethylglycine"	3.3E-03	3.6E-03	2.9E-03	2.0E-03	4.1E-03	2.1E-03									
3-Aminoisobutyric acid	1.7E-03	1.9E-03	2.7E-03	2.6E-03	2.2E-03	2.1E-03									
3-Aminobutyric acid	N.D.	N.D.	1.6E-03	N.D.	N.D.	N.D.									
2-Aminobutyric acid	1.0E-01	9.5E-02	9.7E-02	9.3E-02	8.3E-02	8.3E-02									
GABA	1.7E-01	1.7E-01	1.6E-01	2.6E-01	2.4E-01	2.3E-01									
Choline	1.2E+00	1.2E+00	1.2E+00	1.2E+00	1.3E+00	1.2E+00									
Ser	4.5E+00	4.8E+00	4.8E+00	5.0E+00	5.0E+00	4.7E+00									
Diethanolamine	1.8E-01	1.6E-01	1.7E-01	1.8E-01	1.7E-01	1.4E-01									
Benzylamine"	7.7E-03	7.2E-03	7.2E-03	8.3E-03	7.8E-03	6.0E-03									
Hypotaurine	1.6E+00	1.5E+00	1.5E+00	1.9E+00	1.8E+00	1.8E+00									
Cytosine	3.0E-04	2.3E-04	3.3E-04	2.1E-04	3.8E-04	2.6E-04									
Histamine	8.8E-05	N.D.	8.7E-05	1.3E-04	2.6E-04	9.8E-05									
Creatinine	1.3E-01	6.4E-02	7.5E-02	1.5E-01	8.7E-02	1.3E-01									
3-Amino-2-piperidone	3.3E-02	3.2E-02	3.0E-02	3.1E-02	3.2E-02	2.8E-02									
Pro	2.3E+01	2.2E+01	2.2E+01	2.2E+01	2.0E+01	1.9E+01									
Guanidoacetic acid	5.3E-04	1.0E-03	9.0E-04	4.5E-04	5.8E-04	5.3E-04									
Val	1.1E+01	1.1E+01	1.1E+01	1.0E+01	9.6E+00	9.2E+00									
5-Aminovaleric acid	8.1E-02	8.0E-02	7.7E-02	1.1E-01	9.5E-02	9.4E-02									
Betaine	2.2E+00	2.3E+00	2.4E+00	1.9E+00	2.0E+00	1.9E+00									
Thr	2.0E+01	2.0E+01	2.0E+01	1.8E+01	1.8E+01	1.7E+01									
2-Methylserine	4.8E-02	5.0E-02	5.2E-02	4.8E-02	4.3E-02	4.5E-02									
Homoserine	5.0E-02	5.7E-02	5.9E-02	5.0E-02	5.0E-02	5.1E-02									
Betaine aldehyde_+H2O	7.1E-04	2.9E-04	9.9E-04	1.3E-03	6.8E-04	2.0E-03									
Anserine_divalent	6.6E-04	1.1E-03	7.3E-04	9.2E-04	8.1E-04	7.1E-04									
Cys	1.1E-02	3.6E-02	4.6E-03	6.2E-03	5.7E-03	2.8E-03									
"2-Amino-2-(hydroxymethyl)-1,3-propanediol"	5.7E-01	3.8E-01	4.4E-01	2.8E-01	1.0E+00	1.6E-01									
Isonicotinamide"	4.9E-04	N.D.	6.5E-04	N.D.	4.8E-04	2.9E-04									
Nicotinamide-2	9.4E-02	7.2E-02	7.6E-02	9.3E-02	7.5E-02	8.1E-02									
Acetylpyrazine	1.1E-02	1.3E-02	1.1E-02	1.2E-02	1.3E-02	1.3E-02									
Nicotinic acid	8.8E-04	N.D.	1.5E-03	N.D.	4.4E-03	N.D.									
Taurine	1.4E-01	1.5E-01	1.4E-01	1.7E-01	1.7E-01	1.9E-01									
Imidazole-4-acetic acid	4.0E-03	3.7E-03	3.4E-03	3.3E-03	3.4E-03	2.9E-03									
Melamine	7.1E-04	5.6E-04	6.3E-04	4.4E-04	1.9E-03	6.1E-04									
"5-Amino-3,4-dihydro-2H-pyrrole-2-carboxylic acid"	1.9E-01	1.7E-01	1.8E-01	1.9E-01	1.7E-01	1.8E-01									
Pyroglutamine	2.0E-03	1.5E-03	1.5E-03	1.9E-03	1.7E-03	2.0E-03									
4-Oxopyrrolidine-2-carboxylic acid	2.3E+00	2.2E+00	2.2E+00	2.1E+00	2.0E+00	1.9E+00									
Pipecolic acid	4.6E-02	5.1E-02	4.8E-02	4.4E-02	4.2E-02	4.3E-02									
trans-Glutaconic acid	3.7E-02	3.8E-02	3.8E-02	3.5E-02	3.2E-02	3.4E-02									
Agmatine	N.D.	N.D.	N.D.	N.D.	8.2E-05	N.D.									
3-Hydroxyproline	6.3E-03	5.8E-03	5.4E-03	5.4E-03	5.8E-03	6.3E-03									
5-Amino-4-oxovaleric acid	1.8E-03	3.3E-03	2.8E-03	9.9E-04	1.6E-03	1.0E-03									
cis-4-Hydroxyproline	8.1E-03	7.9E-03	8.6E-03	8.3E-03	7.2E-03	6.3E-03									
Hydroxyproline	6.9E-01	6.4E-01	6.3E-01	5.0E-01	4.7E-01	4.9E-01									
3-Guanidinopropionic acid-1	3.3E-04	2.3E-04	5.6E-04	3.0E-04	1.1E-03	1.5E-04									
Creatine	4.6E+00	4.5E+00	4.3E+00	4.5E+00	4.3E+00	4.1E+00									
3-Guanidinopropionic acid-2	9.9E-03	1.0E-02	9.6E-03	1.1E-02	1.1E-02	1.1E-02									
Leu	2.0E+01	1.9E+01	1.9E+01	1.7E+01	1.7E+01	1.6E+01									
Ile	1.6E+01	1.6E+01	1.6E+01	1.5E+01	1.4E+01	1.4E+01									
6-Aminohexanoic acid	N.D.	N.D.	1.0E-03	1.1E-03	1.4E-03	1.1E-03									
Asn	3.4E+00	3.3E+00	3.2E+00	3.3E+00	3.0E+00	2.9E+00									
Gly-Gly	1.2E-01	1.3E-01	1.2E-01	1.1E-01	1.1E-01	9.9E-02									
Ornithine	1.7E-01	1.8E-01	1.8E-01	1.7E-01	1.8E-01	1.7E-01									
Thiaproline	3.2E-01	7.4E-01	4.6E-01	2.3E-01	8.2E-01	1.9E-01									
Asp	5.7E+00	5.4E+00	5.4E+00	6.5E+00	6.2E+00	6.1E+00									
Diisopropanolamine	9.1E-04	8.3E-04	8.9E-04	1.1E-03	8.6E-04	7.7E-04									
Homocysteine	6.3E-04	6.7E-04	3.4E-04	2.7E-04	2.9E-04	1.3E-04									
S-Methylcysteine	4.2E-03	3.4E-03	3.2E-03	3.2E-03	2.5E-03	3.1E-03									
Adenine	1.1E-02	4.6E-02	4.3E-02	1.1E-02	4.5E-02	1.2E-02									
Hypoxanthine	2.3E-02	2.2E-02	2.2E-02	1.8E-02	1.8E-02	1.6E-02									
1-Methylnicotinamide	1.9E+00	1.9E+00	1.9E+00	2.0E+00	1.9E+00	1.8E+00									
Trigonelline	4.9E-03	1.2E-02	1.5E-02	6.0E-03	7.9E-03	5.8E-03									
Tyramine	N.D.	2.1E-03	1.3E-03	N.D.	N.D.	N.D.									
γ-Glu-Lys_divalent	1.1E-03	1.8E-03	1.3E-03	8.7E-04	1.1E-03	1.0E-03									
Urocanic acid	5.4E-03	7.8E-03	1.3E-02	3.9E-03	1.5E-02	4.4E-03									
1-Methyl-4-imidazoleacetic acid	2.9E-03	3.8E-03	5.9E-03	2.4E-03	7.3E-03	2.1E-03									
1H-Imidazole-4-propionic acid	2.2E-03	3.3E-03	2.2E-03	1.9E-03	1.9E-03	1.8E-03									
Hexamine	N.D.	N.D.	N.D.	3.2E-04	1.7E-03	6.7E-04									
Tropine	1.9E-04	8.6E-04	7.5E-04	1.6E-04	1.4E-04	1.5E-04									
Ectoine	2.3E-03	9.7E-04	1.2E-03	5.9E-04	1.6E-03	4.2E-04									
4-Methyl-5-thiazoleethanol	1.5E-03	N.D.	N.D.	1.1E-03	4.6E-04	8.0E-04									
Crotonic acid betaine	5.9E-03	5.3E-03	5.0E-03	5.7E-03	6.0E-03	5.1E-03									
Stachydrine	1.2E-03	1.7E-03	1.9E-03	N.D.	1.7E-03	N.D.									
4-Guanidinobutyric acid	1.4E-02	1.7E-02	2.7E-02	1.2E-02	1.6E-02	1.4E-02									
Acetylcholine	7.5E-04	N.D.	5.7E-04	8.8E-04	8.0E-04	8.3E-04									
γ-Butyrobetaine	1.6E-01	1.6E-01	1.6E-01	1.6E-01	1.6E-01	1.4E-01									
Spermidine	1.1E-01	9.5E-02	9.6E-02	9.9E-02	9.0E-02	8.9E-02									
Gln	2.9E+01	2.8E+01	2.8E+01	2.7E+01	2.7E+01	2.5E+01									
Glycylsarcosine	1.5E-03	1.4E-03	1.3E-03	1.5E-03	1.4E-03	1.3E-03									
Lys	4.0E+00	4.1E+00	3.9E+00	3.7E+00	3.7E+00	3.5E+00									
2-Methylthiazolidine-4-carboxylic acid	7.2E-03	2.3E-02	2.3E-03	2.6E-03	7.1E-03	1.9E-03									
Glu	5.8E+01	5.5E+01	5.6E+01	5.4E+01	5.0E+01	5.0E+01									
N-Acetylserine	2.6E-01	2.4E-01	2.5E-01	2.5E-01	2.5E-01	2.5E-01									
Met	3.9E+00	3.6E+00	3.8E+00	3.1E+00	2.8E+00	2.9E+00									
Penicillamine	3.9E-02	4.4E-02	4.4E-02	4.6E-02	4.7E-02	3.8E-02									
Triethanolamine	7.8E-03	8.4E-03	8.8E-03	7.6E-03	1.9E-02	6.6E-03									
Guanine	5.6E-03	5.1E-03	6.0E-03	4.8E-03	5.2E-03	5.2E-03									
γ-Glu-Arg_divalent	9.3E-04	1.3E-03	8.3E-04	9.1E-04	1.1E-03	7.2E-04									
Xanthine	1.3E-02	1.4E-02	1.2E-02	1.0E-02	9.6E-03	9.2E-03									
Dopamine"	7.4E-03	1.3E-02	1.0E-02	1.0E-02	1.1E-02	1.0E-02									
His	4.8E+00	4.5E+00	4.5E+00	4.2E+00	4.0E+00	3.7E+00									
Imidazolelactic acid	1.2E-03	1.0E-03	1.0E-03	9.8E-04	9.0E-04	8.5E-04									
Tranexamic acid	N.D.	N.D.	4.9E-03	4.3E-03	3.4E-03	4.5E-03									
2-Aminooctanoic acid	N.D.	5.5E-04	5.7E-04	4.9E-04	2.4E-04	8.5E-04									
4-Acetamido-2-aminobutyric acid	5.6E-04	5.8E-04	5.7E-04	4.0E-04	4.6E-04	3.6E-04									
Ala-Ala	2.1E-02	1.3E-02	1.2E-02	1.7E-02	1.3E-02	1.1E-02									
Tryptamine	2.8E-04	9.9E-05	N.D.	1.9E-04	1.6E-03	1.1E-04									
N6-Methyllysine	9.5E-03	9.2E-03	9.9E-03	9.1E-03	9.1E-03	8.9E-03									
S-Allylcysteine	5.8E-04	N.D.	1.9E-04	2.7E-04	5.4E-04	2.1E-04									
2-Aminoadipic acid"	2.3E-01	2.2E-01	2.2E-01	3.1E-01	2.9E-01	2.9E-01									
O-Acetylhomoserine	6.5E-03	4.7E-03	3.5E-03	6.0E-03	4.6E-03	5.0E-03									
Carnitine	8.4E-01	7.8E-01	8.2E-01	9.9E-01	9.1E-01	8.8E-01									
5-Hydroxylysine	9.0E-03	8.5E-03	8.7E-03	7.7E-03	7.3E-03	6.8E-03									
Nicotine	8.4E-05	5.2E-04	1.9E-04	N.D.	N.D.	N.D.									
S-Methylmethionine	N.D.	1.4E-04	N.D.	9.2E-05	N.D.	N.D.									
Methionine sulfoxide	2.3E-01	3.0E-01	2.8E-01	2.8E-01	2.8E-01	2.6E-01									
3-Methylguanine	N.D.	N.D.	N.D.	N.D.	5.7E-05	N.D.									
7-Methylguanine	1.5E-04	2.3E-04	1.6E-04	1.3E-04	1.2E-04	1.8E-04									
Phe	1.7E+01	1.7E+01	1.7E+01	1.6E+01	1.5E+01	1.4E+01									
Normetanephrine_-H2O	N.D.	N.D.	2.5E-04	N.D.	N.D.	N.D.									
Pyridoxal	3.9E-02	3.8E-02	2.8E-02	2.9E-02	3.6E-02	2.5E-02									
Pyridoxamine	6.5E-03	1.3E-03	3.3E-03	7.4E-03	2.9E-03	5.3E-03									
Tyr-Arg_divalent	2.9E-03	2.3E-03	2.4E-03	2.1E-03	2.0E-03	1.2E-03									
Pyridoxine	1.7E-01	1.8E-01	1.6E-01	1.5E-01	1.4E-01	1.4E-01									
3-Methylhistidine"	5.3E-02	4.6E-02	4.8E-02	4.6E-02	4.1E-02	4.2E-02									
N-Acetylornithine	5.4E-02	4.3E-02	3.8E-02	5.2E-02	3.8E-02	3.3E-02									
Arg	3.3E+00	3.2E+00	3.1E+00	3.2E+00	3.0E+00	2.9E+00									
"N6,N6-Dimethyllysine"	7.7E-03	7.0E-03	6.7E-03	7.3E-03	5.7E-03	7.3E-03									
Citrulline	1.0E-01	1.1E-01	1.1E-01	9.0E-02	9.6E-02	8.9E-02									
Argininic acid	N.D.	N.D.	7.4E-05	8.1E-05	N.D.	N.D.									
Serotonin	6.3E-04	8.3E-04	5.5E-04	7.0E-04	8.0E-04	6.2E-04									
Alliin	7.2E-03	6.6E-03	6.7E-03	4.9E-03	4.3E-03	5.0E-03									
Cys-Gly	4.3E-04	1.1E-03	2.7E-04	3.6E-04	3.8E-04	1.2E-04									
Gluconolactone	5.5E-02	5.8E-02	5.9E-02	4.7E-02	4.6E-02	4.6E-02									
S-Carboxymethylcysteine	2.0E-03	2.7E-03	2.7E-03	1.8E-03	2.2E-03	1.9E-03									
Mannosamine	6.2E-04	N.D.	4.7E-04	1.3E-03	5.1E-04	1.3E-03									
Glucosamine	2.1E-03	2.4E-03	1.5E-03	2.3E-03	1.8E-03	2.5E-03									
Tyr	1.1E+01	1.1E+01	1.1E+01	1.0E+01	9.9E+00	9.6E+00									
Phosphorylcholine	9.9E+00	9.3E+00	9.2E+00	9.3E+00	8.8E+00	8.8E+00									
N8-Acetylspermidine	8.9E-04	1.0E-03	1.1E-03	5.6E-04	7.0E-04	6.0E-04									
N1-Acetylspermidine	2.6E-03	2.4E-03	2.3E-03	1.9E-03	2.4E-03	2.3E-03									
Gly-Hyp	1.0E-02	8.7E-03	9.8E-03	5.4E-03	4.8E-03	4.7E-03									
N6-Acetyllysine	1.8E-02	1.6E-02	1.7E-02	1.6E-02	1.4E-02	1.5E-02									
N-Acetyllysine	9.6E-02	7.1E-02	6.5E-02	7.8E-02	5.7E-02	5.4E-02									
Nω-Methylarginine	2.2E-03	2.1E-03	2.2E-03	1.8E-03	2.1E-03	1.8E-03									
Homoarginine	1.6E-03	1.6E-03	1.5E-03	1.5E-03	1.2E-03	1.2E-03									
"N6,N6,N6-Trimethyllysine"	4.8E-02	4.2E-02	4.2E-02	4.5E-02	3.8E-02	3.9E-02									
Gly-Gly-Gly	1.3E-02	1.2E-02	1.2E-02	1.2E-02	1.1E-02	1.1E-02									
Homocitrulline	3.1E-03	2.7E-03	3.0E-03	2.4E-03	2.4E-03	2.4E-03									
Gly-Asp	3.7E-01	3.8E-01	3.8E-01	3.4E-01	3.3E-01	3.1E-01									
H-Asp(Gly-OH)-OH	7.3E-02	7.1E-02	6.9E-02	8.2E-02	7.6E-02	7.3E-02									
"2,6-Diaminopimelic acid"	N.D.	N.D.	N.D.	N.D.	N.D.	1.2E-04									
γ-Carboxyglutamic acid	7.7E-04	1.1E-03	6.8E-04	8.5E-04	1.0E-03	8.0E-04									
S-(2-Carboxyethyl)cysteine	3.3E-02	3.0E-02	3.4E-02	2.8E-02	2.7E-02	2.7E-02									
Tyrosine methyl ester	4.2E-03	2.0E-03	1.8E-03	4.6E-03	3.5E-03	3.8E-03									
N-Acetylhistidine	1.0E-03	9.3E-04	1.3E-03	9.6E-04	1.1E-03	1.1E-03									
Hercynine	N.D.	N.D.	5.8E-05	N.D.	3.2E-04	N.D.									
Ala-Hyp	3.2E-02	3.0E-02	3.0E-02	2.5E-02	2.3E-02	2.4E-02									
Arginine ethyl ester	7.9E-05	N.D.	N.D.	N.D.	9.4E-05	N.D.									
SDMA	3.9E-03	3.4E-03	3.2E-03	3.2E-03	2.9E-03	2.7E-03									
ADMA	2.7E-02	2.4E-02	2.4E-02	2.1E-02	2.1E-02	1.9E-02									
Spermine	6.2E-02	4.4E-02	5.3E-02	4.9E-02	5.1E-02	4.3E-02									
O-Acetylcarnitine	4.3E-01	4.1E-01	4.4E-01	3.7E-01	3.5E-01	3.8E-01									
γ-Glu-Gly	4.5E-02	4.0E-02	4.2E-02	4.8E-02	4.4E-02	4.4E-02									
Trp	2.4E+00	2.4E+00	2.4E+00	2.1E+00	2.0E+00	1.9E+00									
Carboxymethyllysine	5.0E-03	4.8E-03	5.4E-03	5.2E-03	5.2E-03	4.7E-03									
Kynurenine	1.7E-02	1.8E-02	1.7E-02	1.5E-02	1.5E-02	1.4E-02									
3-(3,4-Dihydroxyphenyl)-2-methylalanine"	3.3E-04	4.2E-04	2.0E-04	2.8E-04	2.4E-04	3.0E-04									
Glycerophosphorylethanolamine	2.0E+00	2.0E+00	2.0E+00	1.9E+00	1.8E+00	1.8E+00									
N-α-Acetylarginine	1.2E-03	1.1E-03	1.4E-03	1.4E-03	1.4E-03	1.3E-03									
Propionylcarnitine	1.9E-01	1.9E-01	1.8E-01	2.5E-01	2.2E-01	2.0E-01									
β-Ala-Lys	4.9E-04	4.0E-04	4.3E-04	3.7E-04	4.5E-04	4.0E-04									
γ-Glu-Ala	7.2E-03	6.4E-03	6.0E-03	7.7E-03	5.9E-03	6.7E-03									
N-Acetylglucosylamine	1.0E-03	1.1E-03	5.4E-04	7.9E-04	9.8E-04	6.8E-04									
N-Acetylgalactosamine	2.9E-03	3.2E-03	2.2E-03	1.3E-03	1.0E-03	3.6E-03									
Cystathionine	1.9E+00	1.8E+00	1.8E+00	1.6E+00	1.5E+00	1.4E+00									
Carnosine	8.4E-03	9.1E-03	8.8E-03	9.2E-03	8.8E-03	8.9E-03									
2'-Deoxycytidine	9.7E-03	9.6E-03	1.0E-02	8.1E-03	8.9E-03	7.6E-03									
Pro-Hyp	1.5E-01	1.5E-01	1.5E-01	9.2E-02	8.4E-02	8.9E-02									
Butyrylcarnitine	7.3E-02	7.2E-02	7.9E-02	7.1E-02	7.0E-02	6.9E-02									
Nω-(Carboxymethyl)arginine	N.D.	1.7E-04	1.3E-04	1.4E-04	1.6E-04	N.D.									
Thr-Asp	1.2E-02	9.1E-03	9.9E-03	9.8E-03	6.6E-03	9.3E-03									
Ser-Glu	3.5E-01	2.7E-01	2.8E-01	3.1E-01	2.3E-01	2.4E-01									
γ-Glu-Ser	1.2E-02	1.2E-02	1.2E-02	1.3E-02	1.3E-02	1.2E-02									
Lidocaine	N.D.	N.D.	N.D.	N.D.	1.6E-04	N.D.									
N’-Formylkynurenine	1.9E-03	1.1E-03	1.1E-03	1.6E-03	2.4E-03	2.4E-03									
N-(1-Deoxy-1-fructosyl)glycine	6.4E-02	3.9E-02	4.1E-02	5.9E-02	4.4E-02	5.5E-02									
Homocarnosine	1.6E-04	N.D.	1.3E-04	2.4E-04	1.2E-04	1.1E-04									
Cytidine	3.4E-02	3.2E-02	3.1E-02	3.3E-02	3.0E-02	3.0E-02									
Tiglylcarnitine	6.2E-03	7.3E-03	6.1E-03	5.3E-03	7.2E-03	4.7E-03									
Isovalerylcarnitine	8.4E-02	8.1E-02	7.9E-02	1.0E-01	9.3E-02	8.8E-02									
γ-Glu-Val	7.0E-02	6.3E-02	6.5E-02	6.5E-02	6.0E-02	6.0E-02									
Pyridoxamine 5'-phosphate	2.8E-02	1.6E-02	1.9E-02	3.1E-02	1.9E-02	2.6E-02									
γ-Glu-Thr	2.3E-02	2.3E-02	2.3E-02	2.3E-02	2.0E-02	2.0E-02									
Pyridoxol 5'-Phosphate	1.5E-03	1.5E-03	2.0E-03	1.4E-03	1.2E-03	1.4E-03									
γ-Glu-Cys	3.5E-02	2.3E-02	1.6E-02	2.7E-02	3.2E-02	1.6E-02									
5'-Deoxyadenosine"	1.1E-03	9.2E-04	1.1E-03	5.4E-04	6.2E-04	6.3E-04									
2'-Deoxyinosine	5.0E-04	3.8E-04	4.4E-04	3.4E-04	2.9E-04	3.1E-04									
5-Methylcytidine	3.2E-04	5.1E-04	4.8E-04	4.3E-04	2.5E-04	3.0E-04									
3-Methylcytidine	1.4E-03	1.2E-03	1.5E-03	1.4E-03	1.3E-03	1.1E-03									
2'-O-Methylcytidine	2.4E-03	2.5E-03	2.3E-03	2.3E-03	2.3E-03	2.3E-03									
Glycerophosphocholine	7.1E+00	7.0E+00	6.8E+00	6.2E+00	6.0E+00	6.0E+00									
γ-Glu-Leu"	7.2E-02	6.6E-02	6.5E-02	6.6E-02	5.9E-02	6.0E-02									
γ-Glu-Asn	9.1E-04	8.9E-04	8.5E-04	8.4E-04	1.0E-03	8.3E-04									
Succinylcarnitine	1.7E-03	1.5E-03	1.3E-03	1.5E-03	1.5E-03	1.6E-03									
H-Asp(Lys-OH)-OH"	8.7E-03	8.1E-03	9.3E-03	1.1E-02	9.8E-03	9.6E-03									
3-Hydroxyisovalerylcarnitine	1.5E-02	1.8E-02	1.7E-02	2.0E-02	2.0E-02	1.8E-02									
γ-Glu-Asp	2.2E-02	2.2E-02	2.2E-02	2.6E-02	2.4E-02	2.4E-02									
Thiamine	1.9E-01	2.2E-01	2.1E-01	1.8E-01	1.8E-01	1.6E-01									
Subaphylline	8.7E-03	7.3E-03	6.5E-03	6.7E-03	6.4E-03	4.9E-03									
2'-Deoxyguanosine	1.2E-03	1.0E-03	9.3E-04	1.2E-03	1.3E-03	9.7E-04									
Adenosine	1.2E-01	1.1E-01	1.2E-01	1.1E-01	1.2E-01	8.5E-02									
Inosine	4.3E-02	4.6E-02	4.8E-02	4.2E-02	4.2E-02	3.4E-02									
Arabinosylhypoxanthine	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.									
N-Acetylcarnosine	N.D.	1.8E-04	1.5E-04	N.D.	N.D.	N.D.									
Glutarylcarnitine	9.7E-04	1.6E-03	N.D.	1.3E-03	1.5E-03	1.3E-03									
Glu-Glu	2.4E-02	2.5E-02	2.7E-02	2.7E-02	2.6E-02	2.1E-02									
γ-Glu-Glu	5.0E-02	4.9E-02	5.1E-02	4.2E-02	3.9E-02	4.2E-02									
Saccharopine	4.0E-02	3.9E-02	4.1E-02	5.0E-02	5.0E-02	4.7E-02									
γ-Glu-Met	5.2E-04	7.6E-04	6.6E-04	2.9E-04	4.1E-04	4.6E-04									
N-(1-Deoxy-1-fructosyl)valine	1.7E-02	5.5E-03	5.5E-03	1.9E-02	1.1E-02	1.9E-02									
N-(1-Deoxy-1-fructosyl)threonine	3.6E-02	6.8E-03	7.8E-03	3.6E-02	1.9E-02	3.6E-02									
1-Methyladenosine	9.9E-03	8.7E-03	8.6E-03	9.8E-03	9.4E-03	8.8E-03									
Guanosine	3.5E-02	3.6E-02	3.7E-02	3.2E-02	3.1E-02	2.8E-02									
His-Glu	5.6E-03	4.8E-03	5.1E-03	4.4E-03	4.3E-03	4.0E-03									
γ-Glu-His	4.9E-04	4.8E-04	4.7E-04	3.9E-04	7.5E-04	3.4E-04									
Gly-Pro-Hyp	4.2E-02	4.0E-02	4.0E-02	2.7E-02	2.8E-02	2.4E-02									
Octanoylcarnitine	1.3E-03	N.D.	1.9E-03	2.1E-03	1.6E-03	1.5E-03									
Ophthalmic acid	6.0E-02	5.7E-02	6.1E-02	6.6E-02	5.5E-02	6.1E-02									
Argininosuccinic acid	2.3E-01	2.1E-01	2.2E-01	2.2E-01	1.9E-01	2.0E-01									
N-(1-Deoxy-1-fructosyl)leucine	3.3E-02	1.5E-02	1.6E-02	3.4E-02	2.4E-02	3.5E-02									
γ-Glu-Phe	7.8E-03	8.0E-03	8.1E-03	7.4E-03	7.3E-03	7.8E-03									
5'-Deoxy-5'-methylthioadenosine	8.5E-02	5.3E-02	6.0E-02	7.5E-02	7.0E-02	8.6E-02									
N1-Methylguanosine	2.4E-03	2.3E-03	2.0E-03	2.4E-03	2.2E-03	2.1E-03									
Streptomycin sulfate_+H2O_divalent	3.5E-03	4.4E-03	3.0E-03	3.0E-03	3.6E-03	3.6E-03									
γ-Glu-Val-Gly	3.1E-03	3.9E-03	4.2E-03	4.2E-03	3.8E-03	4.1E-03									
Arg-Glu	8.5E-03	7.0E-03	7.5E-03	7.4E-03	6.7E-03	6.3E-03									
Glutathione (GSSG)_divalent	1.2E+01	1.6E+01	1.8E+01	1.4E+01	1.1E+01	1.7E+01									
Glutathione (GSH)	2.0E+01	1.4E+01	1.2E+01	1.7E+01	1.9E+01	9.5E+00									
Tyr-Glu	3.9E-03	3.4E-03	2.5E-03	3.2E-03	2.3E-03	2.0E-03									
γ-Glu-Tyr	1.7E-03	2.1E-03	2.8E-03	1.4E-03	1.6E-03	1.5E-03									
"N2,N2-Dimethylguanosine"	4.7E-04	5.9E-04	3.9E-04	3.4E-04	4.7E-04	3.8E-04									
Val-Pro-Pro	3.0E-04	3.7E-04	4.4E-04	4.8E-04	4.4E-04	5.4E-04									
S-Methylglutathione	1.8E-01	5.4E-03	1.4E-02	1.4E-01	5.7E-02	1.1E-01									
Ile-Pro-Pro	1.6E-03	1.3E-03	1.2E-03	1.4E-03	1.3E-03	9.9E-04									
γ-Glu-Trp	1.0E-03	8.6E-04	8.7E-04	1.0E-03	7.7E-04	8.0E-04									
NMN	6.4E-03	6.5E-03	6.5E-03	5.3E-03	6.6E-03	5.3E-03									
S-Nitrosoglutathione	1.5E-03	1.2E-03	7.4E-04	1.8E-03	1.9E-03	5.3E-04									
Lauroylcarnitine	2.9E-04	N.D.	3.9E-04	6.2E-04	3.8E-04	N.D.									
Thiamine phosphate	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.									
Riboflavin	1.7E-02	5.2E-03	9.7E-03	2.8E-02	7.2E-03	6.5E-03									
S-Lactoylglutathione	2.2E-02	4.5E-03	1.5E-02	3.0E-02	2.5E-02	2.8E-02									
Succinyladenosine	2.2E-03	2.1E-03	2.0E-03	1.8E-03	1.8E-03	1.9E-03									
S-Adenosylhomocysteine	7.3E-02	5.6E-03	9.0E-03	5.8E-02	2.6E-02	4.7E-02									
S-Adenosylmethionine	1.7E-01	2.2E-01	2.3E-01	1.6E-01	1.7E-01	1.5E-01									
Leu-Leu-Tyr	N.D.	1.8E-04	N.D.	1.4E-04	N.D.	N.D.									
Cysteine glutathione disulfide	7.2E-03	6.0E-02	1.0E-02	5.3E-03	3.9E-03	2.3E-02									
Folic acid	7.2E-04	1.4E-03	7.2E-04	2.5E-04	6.5E-04	3.5E-04									
CDP-ethanolamine	7.1E-02	7.1E-02	7.3E-02	8.2E-02	8.7E-02	8.6E-02									
MS_METABOLITE_DATA_END
#METABOLITES
METABOLITES_START
metabolite_name
Urea
Ethanolamine
Aminoacetone
Methylguanidine
Isobutylamine
Gly
Trimethylamine N-oxide
Isopropanolamine
Piperidine
Morpholine
Putrescine
Ala
β-Ala
Sarcosine
2-Amino-2-methyl-1-propanol
Glycerol
Imidazole-4-methanol
1-Methyl-2-pyrrolidone
Cyclohexylamine
Azetidine 2-carboxylic acid
Homoserinelactone
Cadaverine-1"
Cadaverine-2"
"N,N-Dimethylglycine"
3-Aminoisobutyric acid
3-Aminobutyric acid
2-Aminobutyric acid
GABA
Choline
Ser
Diethanolamine
Benzylamine"
Hypotaurine
Cytosine
Histamine
Creatinine
3-Amino-2-piperidone
Pro
Guanidoacetic acid
Val
5-Aminovaleric acid
Betaine
Thr
2-Methylserine
Homoserine
Betaine aldehyde_+H2O
Anserine_divalent
Cys
"2-Amino-2-(hydroxymethyl)-1,3-propanediol"
Isonicotinamide"
Nicotinamide-2
Acetylpyrazine
Nicotinic acid
Taurine
Imidazole-4-acetic acid
Melamine
"5-Amino-3,4-dihydro-2H-pyrrole-2-carboxylic acid"
Pyroglutamine
4-Oxopyrrolidine-2-carboxylic acid
Pipecolic acid
trans-Glutaconic acid
Agmatine
3-Hydroxyproline
5-Amino-4-oxovaleric acid
cis-4-Hydroxyproline
Hydroxyproline
3-Guanidinopropionic acid-1
Creatine
3-Guanidinopropionic acid-2
Leu
Ile
6-Aminohexanoic acid
Asn
Gly-Gly
Ornithine
Thiaproline
Asp
Diisopropanolamine
Homocysteine
S-Methylcysteine
Adenine
Hypoxanthine
1-Methylnicotinamide
Trigonelline
Tyramine
γ-Glu-Lys_divalent
Urocanic acid
1-Methyl-4-imidazoleacetic acid
1H-Imidazole-4-propionic acid
Hexamine
Tropine
Ectoine
4-Methyl-5-thiazoleethanol
Crotonic acid betaine
Stachydrine
4-Guanidinobutyric acid
Acetylcholine
γ-Butyrobetaine
Spermidine
Gln
Glycylsarcosine
Lys
2-Methylthiazolidine-4-carboxylic acid
Glu
N-Acetylserine
Met
Penicillamine
Triethanolamine
Guanine
γ-Glu-Arg_divalent
Xanthine
Dopamine"
His
Imidazolelactic acid
Tranexamic acid
2-Aminooctanoic acid
4-Acetamido-2-aminobutyric acid
Ala-Ala
Tryptamine
N6-Methyllysine
S-Allylcysteine
2-Aminoadipic acid"
O-Acetylhomoserine
Carnitine
5-Hydroxylysine
Nicotine
S-Methylmethionine
Methionine sulfoxide
3-Methylguanine
7-Methylguanine
Phe
Normetanephrine_-H2O
Pyridoxal
Pyridoxamine
Tyr-Arg_divalent
Pyridoxine
3-Methylhistidine"
N-Acetylornithine
Arg
"N6,N6-Dimethyllysine"
Citrulline
Argininic acid
Serotonin
Alliin
Cys-Gly
Gluconolactone
S-Carboxymethylcysteine
Mannosamine
Glucosamine
Tyr
Phosphorylcholine
N8-Acetylspermidine
N1-Acetylspermidine
Gly-Hyp
N6-Acetyllysine
N-Acetyllysine
Nω-Methylarginine
Homoarginine
"N6,N6,N6-Trimethyllysine"
Gly-Gly-Gly
Homocitrulline
Gly-Asp
H-Asp(Gly-OH)-OH
"2,6-Diaminopimelic acid"
γ-Carboxyglutamic acid
S-(2-Carboxyethyl)cysteine
Tyrosine methyl ester
N-Acetylhistidine
Hercynine
Ala-Hyp
Arginine ethyl ester
SDMA
ADMA
Spermine
O-Acetylcarnitine
γ-Glu-Gly
Trp
Carboxymethyllysine
Kynurenine
3-(3,4-Dihydroxyphenyl)-2-methylalanine"
Glycerophosphorylethanolamine
N-α-Acetylarginine
Propionylcarnitine
β-Ala-Lys
γ-Glu-Ala
N-Acetylglucosylamine
N-Acetylgalactosamine
Cystathionine
Carnosine
2'-Deoxycytidine
Pro-Hyp
Butyrylcarnitine
Nω-(Carboxymethyl)arginine
Thr-Asp
Ser-Glu
γ-Glu-Ser
Lidocaine
N’-Formylkynurenine
N-(1-Deoxy-1-fructosyl)glycine
Homocarnosine
Cytidine
Tiglylcarnitine
Isovalerylcarnitine
γ-Glu-Val
Pyridoxamine 5'-phosphate
γ-Glu-Thr
Pyridoxol 5'-Phosphate
γ-Glu-Cys
5'-Deoxyadenosine"
2'-Deoxyinosine
5-Methylcytidine
3-Methylcytidine
2'-O-Methylcytidine
Glycerophosphocholine
γ-Glu-Leu"
γ-Glu-Asn
Succinylcarnitine
H-Asp(Lys-OH)-OH"
3-Hydroxyisovalerylcarnitine
γ-Glu-Asp
Thiamine
Subaphylline
2'-Deoxyguanosine
Adenosine
Inosine
Arabinosylhypoxanthine
N-Acetylcarnosine
Glutarylcarnitine
Glu-Glu
γ-Glu-Glu
Saccharopine
γ-Glu-Met
N-(1-Deoxy-1-fructosyl)valine
N-(1-Deoxy-1-fructosyl)threonine
1-Methyladenosine
Guanosine
His-Glu
γ-Glu-His
Gly-Pro-Hyp
Octanoylcarnitine
Ophthalmic acid
Argininosuccinic acid
N-(1-Deoxy-1-fructosyl)leucine
γ-Glu-Phe
5'-Deoxy-5'-methylthioadenosine
N1-Methylguanosine
Streptomycin sulfate_+H2O_divalent
γ-Glu-Val-Gly
Arg-Glu
Glutathione (GSSG)_divalent
Glutathione (GSH)
Tyr-Glu
γ-Glu-Tyr
"N2,N2-Dimethylguanosine"
Val-Pro-Pro
S-Methylglutathione
Ile-Pro-Pro
γ-Glu-Trp
NMN
S-Nitrosoglutathione
Lauroylcarnitine
Thiamine phosphate
Riboflavin
S-Lactoylglutathione
Succinyladenosine
S-Adenosylhomocysteine
S-Adenosylmethionine
Leu-Leu-Tyr
Cysteine glutathione disulfide
Folic acid
CDP-ethanolamine
METABOLITES_END
#END