#METABOLOMICS WORKBENCH jrdiefe1_20250729_105203 DATATRACK_ID:6229 STUDY_ID:ST004091 ANALYSIS_ID:AN006793 PROJECT_ID:PR002570
VERSION             	1
CREATED_ON             	August 7, 2025, 12:48 pm
#PROJECT
PR:PROJECT_TITLE                 	The Chromobacterium Volatilome is Strongly Influenced by Growth on Liquid versus
PR:PROJECT_TITLE                 	Solid Media
PR:PROJECT_TYPE                  	Untargeted metabolomics
PR:PROJECT_SUMMARY               	The study of microbial volatile organic compounds (mVOCs) is a growing area of
PR:PROJECT_SUMMARY               	research, with applications ranging from agriculture to human health. The
PR:PROJECT_SUMMARY               	majority of the mVOC data are from in vitro liquid cultures, while few analyses
PR:PROJECT_SUMMARY               	of bacterial and fungal volatilomes on solid media cultures exist. Studies
PR:PROJECT_SUMMARY               	comparing liquid versus solid cultures of bacteria and fungi show significant
PR:PROJECT_SUMMARY               	changes to the soluble metabolites that are produced, suggesting that large
PR:PROJECT_SUMMARY               	differences would be observed for mVOCs based on the culture conditions. In this
PR:PROJECT_SUMMARY               	study, we conducted an untargeted analysis of the volatile profiles of two
PR:PROJECT_SUMMARY               	species of Chromobacterium, C. violaceum (strain ATCC12472) and C. vaccinii
PR:PROJECT_SUMMARY               	(strain MWU328), and those of their isogenic cviR− quorum-sensing mutants
PR:PROJECT_SUMMARY               	cultured on solid and liquid King’s Medium B media.
PR:INSTITUTE                     	Arizona State University
PR:DEPARTMENT                    	School of Life Sciences
PR:LABORATORY                    	Bean Laboratory
PR:LAST_NAME                     	Bean
PR:FIRST_NAME                    	Heather
PR:ADDRESS                       	PO Box 874501, Tempe AZ, 85287, USA
PR:EMAIL                         	Heather.D.Bean@asu.edu
PR:PHONE                         	480-727-3395
#STUDY
ST:STUDY_TITLE                   	The Chromobacterium Volatilome is Strongly Influenced by Growth on Liquid versus
ST:STUDY_TITLE                   	Solid Media
ST:STUDY_TYPE                    	Untargeted metabolomics
ST:STUDY_SUMMARY                 	The study of microbial volatile organic compounds (mVOCs) is a growing area of
ST:STUDY_SUMMARY                 	research, with applications ranging from agriculture to human health. The
ST:STUDY_SUMMARY                 	majority of the mVOC data are from in vitro liquid cultures, while few analyses
ST:STUDY_SUMMARY                 	of bacterial and fungal volatilomes on solid media cultures exist. Studies
ST:STUDY_SUMMARY                 	comparing liquid versus solid cultures of bacteria and fungi show significant
ST:STUDY_SUMMARY                 	changes to the soluble metabolites that are produced, suggesting that large
ST:STUDY_SUMMARY                 	differences would be observed for mVOCs based on the culture conditions. To test
ST:STUDY_SUMMARY                 	this idea, we characterized the volatilomes of two species of Chromobacterium,
ST:STUDY_SUMMARY                 	C. violaceum (strain ATCC12472) and C. vaccinii (strain MWU328), and those of
ST:STUDY_SUMMARY                 	their isogenic cviR− quorum-sensing mutants cultured on solid versus liquid
ST:STUDY_SUMMARY                 	King’s Medium B media. VOCs were sampled using thin-film solid-phase
ST:STUDY_SUMMARY                 	microextraction (TF-SPME) and analyzed by two-dimensional gas
ST:STUDY_SUMMARY                 	chromatography–time-of-flight mass spectrometry (GC×GC-TOFMS). Of the three
ST:STUDY_SUMMARY                 	variables examined – Chromobacterium species, media type, and quorum sensing
ST:STUDY_SUMMARY                 	ability – growth on liquid versus solid media caused the most significant
ST:STUDY_SUMMARY                 	differences in the volatilomes. Bacterial species and strain were also
ST:STUDY_SUMMARY                 	influential, but to a lesser degree. Our findings indicate the importance of
ST:STUDY_SUMMARY                 	growth conditions in microbial volatilomics, and therefore, more consideration
ST:STUDY_SUMMARY                 	should be given to how microorganisms are cultured for volatilome analyses and
ST:STUDY_SUMMARY                 	how those conditions are reported.
ST:INSTITUTE                     	Arizona State University
ST:LAST_NAME                     	Bean
ST:FIRST_NAME                    	Heather
ST:ADDRESS                       	PO Box 874501, Tempe, AZ, 85287, USA
ST:EMAIL                         	Heather.D.Bean@asu.edu
ST:PHONE                         	480-727-3395
#SUBJECT
SU:SUBJECT_TYPE                  	Bacteria
SU:SUBJECT_SPECIES               	Chromobacterium violaceum; Chromobacterium vaccinii
SU:TAXONOMY_ID                   	536;1108595
SU:CELL_BIOSOURCE_OR_SUPPLIER    	ATCC; Midwestern University
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data
SUBJECT_SAMPLE_FACTORS           	C. violaceum	CV12472_liquid_2B_1	Sample source:Bacteria | Strain:QS+ | Culture medium:KMB_broth	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV12472_liquid_2B_1.smp
SUBJECT_SAMPLE_FACTORS           	C. violaceum	CV12472_solid_6A_1	Sample source:Bacteria | Strain:QS+ | Culture medium:KMB_agar	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV12472_solid_6A_1.smp
SUBJECT_SAMPLE_FACTORS           	C. violaceum	CV12472_liquid_3A_1	Sample source:Bacteria | Strain:QS+ | Culture medium:KMB_broth	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV12472_liquid_3A_1.smp
SUBJECT_SAMPLE_FACTORS           	Media	KMB_liquid_1B_1	Sample source:Blank | Strain:NA | Culture medium:KMB_broth	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=KMB_liquid_1B_1.smp
SUBJECT_SAMPLE_FACTORS           	C. vaccinii	CV328_solid_4B_1	Sample source:Bacteria | Strain:QS+ | Culture medium:KMB_agar	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV328_solid_4B_1.smp
SUBJECT_SAMPLE_FACTORS           	C. vaccinii	CV328_liquid_1B_1	Sample source:Bacteria | Strain:QS+ | Culture medium:KMB_broth	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV328_liquid_1B_1.smp
SUBJECT_SAMPLE_FACTORS           	C. vaccinii	CV328w_solid_4B_1	Sample source:Bacteria | Strain:cviR- | Culture medium:KMB_agar	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV328w_solid_4B_1.smp
SUBJECT_SAMPLE_FACTORS           	C. vaccinii	CV328_liquid_2C_1	Sample source:Bacteria | Strain:QS+ | Culture medium:KMB_broth	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV328_liquid_2C_1.smp
SUBJECT_SAMPLE_FACTORS           	Media	KMB_liquid_2A_1	Sample source:Blank | Strain:NA | Culture medium:KMB_broth	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=KMB_liquid_2A_1.smp
SUBJECT_SAMPLE_FACTORS           	Alkane Standard	KI Mix_2	Sample source:NA | Strain:NA | Culture medium:NA	Culture temperature=NA; RAW_FILE_NAME(Raw file name)=KI Mix_2.smp
SUBJECT_SAMPLE_FACTORS           	Empty run	Blank_5	Sample source:NA | Strain:NA | Culture medium:NA	Culture temperature=NA; RAW_FILE_NAME(Raw file name)=Blank_5.smp
SUBJECT_SAMPLE_FACTORS           	C. violaceum	CV12472_solid_2B_1	Sample source:Bacteria | Strain:QS+ | Culture medium:KMB_agar	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV12472_solid_2B_1.smp
SUBJECT_SAMPLE_FACTORS           	Media	KMB_liquid_1C_1	Sample source:Bacteria | Strain:NA | Culture medium:KMB_broth	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=KMB_liquid_1C_1.smp
SUBJECT_SAMPLE_FACTORS           	C. violaceum	CV12472_liquid_4B_1	Sample source:Bacteria | Strain:QS+ | Culture medium:KMB_broth	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV12472_liquid_4B_1.smp
SUBJECT_SAMPLE_FACTORS           	Media	KMB_solid_2C_1	Sample source:Blank | Strain:NA | Culture medium:KMB_agar	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=KMB_solid_2C_1.smp
SUBJECT_SAMPLE_FACTORS           	C. vaccinii	CV328_liquid_2A_1	Sample source:Bacteria | Strain:QS+ | Culture medium:KMB_broth	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV328_liquid_2A_1.smp
SUBJECT_SAMPLE_FACTORS           	C. violaceum	CV12472_liquid_5C_1	Sample source:Bacteria | Strain:QS+ | Culture medium:KMB_broth	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV12472_liquid_5C_1.smp
SUBJECT_SAMPLE_FACTORS           	C. violaceum	CV12472_liquid_4C_1	Sample source:Bacteria | Strain:QS+ | Culture medium:KMB_broth	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV12472_liquid_4C_1.smp
SUBJECT_SAMPLE_FACTORS           	C. vaccinii	CV328_liquid_4A_1	Sample source:Bacteria | Strain:QS+ | Culture medium:KMB_broth	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV328_liquid_4A_1.smp
SUBJECT_SAMPLE_FACTORS           	C. vaccinii	CV328w_liquid_2C_1	Sample source:Bacteria | Strain:cviR- | Culture medium:KMB_broth	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV328w_liquid_2C_1.smp
SUBJECT_SAMPLE_FACTORS           	C. violaceum	CV12472_liquid_4A_1	Sample source:Bacteria | Strain:QS+ | Culture medium:KMB_broth	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV12472_liquid_4A_1.smp
SUBJECT_SAMPLE_FACTORS           	C. vaccinii	CV328_liquid_5A_1	Sample source:Bacteria | Strain:QS+ | Culture medium:KMB_broth	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV328_liquid_5A_1.smp
SUBJECT_SAMPLE_FACTORS           	C. violaceum	CV12472w_liquid_6B_1	Sample source:Bacteria | Strain:cviR- | Culture medium:KMB_broth	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV12472w_liquid_6B_1.smp
SUBJECT_SAMPLE_FACTORS           	C. vaccinii	CV328w_liquid_4C_1	Sample source:Bacteria | Strain:cviR- | Culture medium:KMB_broth	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV328w_liquid_4C_1.smp
SUBJECT_SAMPLE_FACTORS           	C. violaceum	CV12472w_liquid_1A_1	Sample source:Bacteria | Strain:cviR- | Culture medium:KMB_broth	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV12472w_liquid_1A_1.smp
SUBJECT_SAMPLE_FACTORS           	C. vaccinii	CV328w_solid_5B_1	Sample source:Bacteria | Strain:cviR- | Culture medium:KMB_agar	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV328w_solid_5B_1.smp
SUBJECT_SAMPLE_FACTORS           	C. vaccinii	CV328w_liquid_3A_1	Sample source:Bacteria | Strain:cviR- | Culture medium:KMB_broth	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV328w_liquid_3A_1.smp
SUBJECT_SAMPLE_FACTORS           	C. violaceum	CV12472w_solid_5B_1	Sample source:Bacteria | Strain:cviR- | Culture medium:KMB_agar	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV12472w_solid_5B_1.smp
SUBJECT_SAMPLE_FACTORS           	C. vaccinii	CV328w_solid_1C_1	Sample source:Bacteria | Strain:cviR- | Culture medium:KMB_agar	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV328w_solid_1C_1.smp
SUBJECT_SAMPLE_FACTORS           	C. violaceum	CV12472w_liquid_2A_1	Sample source:Bacteria | Strain:cviR- | Culture medium:KMB_broth	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV12472w_liquid_2A_1.smp
SUBJECT_SAMPLE_FACTORS           	C. vaccinii	CV328w_liquid_2B_1	Sample source:Bacteria | Strain:cviR- | Culture medium:KMB_broth	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV328w_liquid_2B_1.smp
SUBJECT_SAMPLE_FACTORS           	C. violaceum	CV12472_liquid_5A_1	Sample source:Bacteria | Strain:QS+ | Culture medium:KMB_broth	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV12472_liquid_5A_1.smp
SUBJECT_SAMPLE_FACTORS           	C. vaccinii	CV328_liquid_6B_1	Sample source:Bacteria | Strain:QS+ | Culture medium:KMB_broth	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV328_liquid_6B_1.smp
SUBJECT_SAMPLE_FACTORS           	Media	KMB_liquid_3A_1	Sample source:Blank | Strain:NA | Culture medium:KMB_broth	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=KMB_liquid_3A_1.smp
SUBJECT_SAMPLE_FACTORS           	C. vaccinii	CV328_liquid_1A_1	Sample source:Bacteria | Strain:QS+ | Culture medium:KMB_broth	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV328_liquid_1A_1.smp
SUBJECT_SAMPLE_FACTORS           	C. violaceum	CV12472_liquid_3B_1	Sample source:Bacteria | Strain:QS+ | Culture medium:KMB_broth	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV12472_liquid_3B_1.smp
SUBJECT_SAMPLE_FACTORS           	C. violaceum	CV12472w_liquid_2B_1	Sample source:Bacteria | Strain:cviR- | Culture medium:KMB_broth	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV12472w_liquid_2B_1.smp
SUBJECT_SAMPLE_FACTORS           	Media	KMB_liquid_2C_1	Sample source:Blank | Strain:NA | Culture medium:KMB_broth	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=KMB_liquid_2C_1.smp
SUBJECT_SAMPLE_FACTORS           	C. violaceum	CV12472_solid_1C_1	Sample source:Bacteria | Strain:QS+ | Culture medium:KMB_agar	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV12472_solid_1C_1.smp
SUBJECT_SAMPLE_FACTORS           	C. violaceum	CV12472_solid_2C_1	Sample source:Bacteria | Strain:QS+ | Culture medium:KMB_agar	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV12472_solid_2C_1.smp
SUBJECT_SAMPLE_FACTORS           	C. violaceum	CV12472_solid_6B_1	Sample source:Bacteria | Strain:QS+ | Culture medium:KMB_agar	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV12472_solid_6B_1.smp
SUBJECT_SAMPLE_FACTORS           	C. vaccinii	CV328_solid_6B_1	Sample source:Bacteria | Strain:QS+ | Culture medium:KMB_agar	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV328_solid_6B_1.smp
SUBJECT_SAMPLE_FACTORS           	C. vaccinii	CV328w_liquid_6B_1	Sample source:Bacteria | Strain:cviR- | Culture medium:KMB_broth	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV328w_liquid_6B_1.smp
SUBJECT_SAMPLE_FACTORS           	C. vaccinii	CV328w_solid_2C_1	Sample source:Bacteria | Strain:cviR- | Culture medium:KMB_agar	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV328w_solid_2C_1.smp
SUBJECT_SAMPLE_FACTORS           	C. violaceum	CV12472_solid_6C_1	Sample source:Bacteria | Strain:QS+ | Culture medium:KMB_agar	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV12472_solid_6C_1.smp
SUBJECT_SAMPLE_FACTORS           	C. violaceum	CV12472w_solid_5C_1	Sample source:Bacteria | Strain:cviR- | Culture medium:KMB_agar	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV12472w_solid_5C_1.smp
SUBJECT_SAMPLE_FACTORS           	C. vaccinii	CV328_solid_1B_1	Sample source:Bacteria | Strain:QS+ | Culture medium:KMB_agar	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV328_solid_1B_1.smp
SUBJECT_SAMPLE_FACTORS           	C. vaccinii	CV328w_solid_6B_1	Sample source:Bacteria | Strain:cviR- | Culture medium:KMB_agar	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV328w_solid_6B_1.smp
SUBJECT_SAMPLE_FACTORS           	C. violaceum	CV12472w_liquid_1B_1	Sample source:Bacteria | Strain:cviR- | Culture medium:KMB_broth	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV12472w_liquid_1B_1.smp
SUBJECT_SAMPLE_FACTORS           	C. violaceum	CV12472_liquid_1B_1	Sample source:Bacteria | Strain:QS+ | Culture medium:KMB_broth	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV12472_liquid_1B_1.smp
SUBJECT_SAMPLE_FACTORS           	Alkane Standard	KI Mix_3	Sample source:NA | Strain:NA | Culture medium:NA	Culture temperature=NA; RAW_FILE_NAME(Raw file name)=KI Mix_3.smp
SUBJECT_SAMPLE_FACTORS           	Empty run	Blank_12	Sample source:NA | Strain:NA | Culture medium:NA	Culture temperature=NA; RAW_FILE_NAME(Raw file name)=Blank_12.smp
SUBJECT_SAMPLE_FACTORS           	C. violaceum	CV12472_solid_3C_1	Sample source:Bacteria | Strain:QS+ | Culture medium:KMB_agar	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV12472_solid_3C_1.smp
SUBJECT_SAMPLE_FACTORS           	C. violaceum	CV12472_liquid_2A_1	Sample source:Bacteria | Strain:QS+ | Culture medium:KMB_broth	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV12472_liquid_2A_1.smp
SUBJECT_SAMPLE_FACTORS           	C. vaccinii	CV328w_liquid_4B_1	Sample source:Bacteria | Strain:cviR- | Culture medium:KMB_broth	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV328w_liquid_4B_1.smp
SUBJECT_SAMPLE_FACTORS           	C. violaceum	CV12472w_solid_4B_1	Sample source:Bacteria | Strain:cviR- | Culture medium:KMB_agar	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV12472w_solid_4B_1.smp
SUBJECT_SAMPLE_FACTORS           	C. vaccinii	CV328_solid_3B_1	Sample source:Bacteria | Strain:QS+ | Culture medium:KMB_agar	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV328_solid_3B_1.smp
SUBJECT_SAMPLE_FACTORS           	C. violaceum	CV12472_solid_3B_1	Sample source:Bacteria | Strain:QS+ | Culture medium:KMB_agar	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV12472_solid_3B_1.smp
SUBJECT_SAMPLE_FACTORS           	C. vaccinii	CV328w_liquid_5C_1	Sample source:Bacteria | Strain:cviR- | Culture medium:KMB_broth	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV328w_liquid_5C_1.smp
SUBJECT_SAMPLE_FACTORS           	C. violaceum	CV12472_solid_1B_1	Sample source:Bacteria | Strain:QS+ | Culture medium:KMB_agar	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV12472_solid_1B_1.smp
SUBJECT_SAMPLE_FACTORS           	C. violaceum	CV12472w_solid_1A_1	Sample source:Bacteria | Strain:cviR- | Culture medium:KMB_agar	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV12472w_solid_1A_1.smp
SUBJECT_SAMPLE_FACTORS           	C. violaceum	CV12472_solid_2A_1	Sample source:Bacteria | Strain:QS+ | Culture medium:KMB_agar	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV12472_solid_2A_1.smp
SUBJECT_SAMPLE_FACTORS           	C. violaceum	CV12472w_solid_4C_1	Sample source:Bacteria | Strain:cviR- | Culture medium:KMB_agar	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV12472w_solid_4C_1.smp
SUBJECT_SAMPLE_FACTORS           	C. vaccinii	CV328_solid_2B_1	Sample source:Bacteria | Strain:QS+ | Culture medium:KMB_agar	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV328_solid_2B_1.smp
SUBJECT_SAMPLE_FACTORS           	C. violaceum	CV12472w_liquid_4C_1	Sample source:Bacteria | Strain:cviR- | Culture medium:KMB_broth	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV12472w_liquid_4C_1.smp
SUBJECT_SAMPLE_FACTORS           	C. violaceum	CV12472w_solid_6B_1	Sample source:Bacteria | Strain:cviR- | Culture medium:KMB_agar	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV12472w_solid_6B_1.smp
SUBJECT_SAMPLE_FACTORS           	C. violaceum	CV12472w_liquid_2C_1	Sample source:Bacteria | Strain:cviR- | Culture medium:KMB_broth	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV12472w_liquid_2C_1.smp
SUBJECT_SAMPLE_FACTORS           	C. violaceum	CV12472w_liquid_5A_1	Sample source:Bacteria | Strain:cviR- | Culture medium:KMB_broth	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV12472w_liquid_5A_1.smp
SUBJECT_SAMPLE_FACTORS           	C. violaceum	CV12472_solid_4B_1	Sample source:Bacteria | Strain:QS+ | Culture medium:KMB_agar	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV12472_solid_4B_1.smp
SUBJECT_SAMPLE_FACTORS           	C. violaceum	CV12472w_solid_2A_1	Sample source:Bacteria | Strain:cviR- | Culture medium:KMB_agar	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV12472w_solid_2A_1.smp
SUBJECT_SAMPLE_FACTORS           	C. violaceum	CV12472w_solid_1B_1	Sample source:Bacteria | Strain:cviR- | Culture medium:KMB_agar	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV12472w_solid_1B_1.smp
SUBJECT_SAMPLE_FACTORS           	C. vaccinii	CV328w_liquid_5B_1	Sample source:Bacteria | Strain:cviR- | Culture medium:KMB_broth	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV328w_liquid_5B_1.smp
SUBJECT_SAMPLE_FACTORS           	C. vaccinii	CV328_solid_5A_1	Sample source:Bacteria | Strain:QS+ | Culture medium:KMB_agar	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV328_solid_5A_1.smp
SUBJECT_SAMPLE_FACTORS           	C. violaceum	CV12472w_liquid_3A_1	Sample source:Bacteria | Strain:cviR- | Culture medium:KMB_broth	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV12472w_liquid_3A_1.smp
SUBJECT_SAMPLE_FACTORS           	C. vaccinii	CV328_solid_2A_1	Sample source:Bacteria | Strain:QS+ | Culture medium:KMB_agar	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV328_solid_2A_1.smp
SUBJECT_SAMPLE_FACTORS           	C. violaceum	CV12472w_liquid_6C_1	Sample source:Bacteria | Strain:cviR- | Culture medium:KMB_broth	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV12472w_liquid_6C_1.smp
SUBJECT_SAMPLE_FACTORS           	C. vaccinii	CV328_solid_4A_1	Sample source:Bacteria | Strain:QS+ | Culture medium:KMB_agar	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV328_solid_4A_1.smp
SUBJECT_SAMPLE_FACTORS           	C. vaccinii	CV328_solid_2C_1	Sample source:Bacteria | Strain:QS+ | Culture medium:KMB_agar	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV328_solid_2C_1.smp
SUBJECT_SAMPLE_FACTORS           	C. violaceum	CV12472w_liquid_4B_1	Sample source:Bacteria | Strain:cviR- | Culture medium:KMB_broth	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV12472w_liquid_4B_1.smp
SUBJECT_SAMPLE_FACTORS           	Media	KMB_solid_3B_1	Sample source:Blank | Strain:NA | Culture medium:KMB_agar	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=KMB_solid_3B_1.smp
SUBJECT_SAMPLE_FACTORS           	C. vaccinii	CV328w_liquid_1A_1	Sample source:Bacteria | Strain:cviR- | Culture medium:KMB_broth	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV328w_liquid_1A_1.smp
SUBJECT_SAMPLE_FACTORS           	C. vaccinii	CV328w_solid_5A_1	Sample source:Bacteria | Strain:cviR- | Culture medium:KMB_agar	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV328w_solid_5A_1.smp
SUBJECT_SAMPLE_FACTORS           	C. vaccinii	CV328w_liquid_2A_1	Sample source:Bacteria | Strain:cviR- | Culture medium:KMB_broth	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV328w_liquid_2A_1.smp
SUBJECT_SAMPLE_FACTORS           	C. vaccinii	CV328w_solid_4C_1	Sample source:Bacteria | Strain:cviR- | Culture medium:KMB_agar	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV328w_solid_4C_1.smp
SUBJECT_SAMPLE_FACTORS           	C. violaceum	CV12472w_solid_4A_1	Sample source:Bacteria | Strain:cviR- | Culture medium:KMB_agar	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV12472w_solid_4A_1.smp
SUBJECT_SAMPLE_FACTORS           	C. violaceum	CV12472_solid_5A_1	Sample source:Bacteria | Strain:QS+ | Culture medium:KMB_agar	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV12472_solid_5A_1.smp
SUBJECT_SAMPLE_FACTORS           	Media	KMB_solid_2B_1	Sample source:Blank | Strain:NA | Culture medium:KMB_agar	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=KMB_solid_2B_1.smp
SUBJECT_SAMPLE_FACTORS           	C. vaccinii	CV328w_solid_4A_1	Sample source:Bacteria | Strain:cviR- | Culture medium:KMB_agar	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV328w_solid_4A_1.smp
SUBJECT_SAMPLE_FACTORS           	C. violaceum	CV12472w_liquid_5B_1	Sample source:Bacteria | Strain:cviR- | Culture medium:KMB_broth	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV12472w_liquid_5B_1.smp
SUBJECT_SAMPLE_FACTORS           	C. vaccinii	CV328_solid_1A_1	Sample source:Bacteria | Strain:QS+ | Culture medium:KMB_agar	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV328_solid_1A_1.smp
SUBJECT_SAMPLE_FACTORS           	C. violaceum	CV12472w_solid_2C_1	Sample source:Bacteria | Strain:cviR- | Culture medium:KMB_agar	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV12472w_solid_2C_1.smp
SUBJECT_SAMPLE_FACTORS           	C. violaceum	CV12472_solid_1A_1	Sample source:Bacteria | Strain:QS+ | Culture medium:KMB_agar	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV12472_solid_1A_1.smp
SUBJECT_SAMPLE_FACTORS           	C. violaceum	CV12472_liquid_1C_1	Sample source:Bacteria | Strain:QS+ | Culture medium:KMB_broth	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV12472_liquid_1C_1.smp
SUBJECT_SAMPLE_FACTORS           	C. vaccinii	CV328w_liquid_5A_1	Sample source:Bacteria | Strain:cviR- | Culture medium:KMB_broth	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV328w_liquid_5A_1.smp
SUBJECT_SAMPLE_FACTORS           	C. violaceum	CV12472w_solid_2B_1	Sample source:Bacteria | Strain:cviR- | Culture medium:KMB_agar	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV12472w_solid_2B_1.smp
SUBJECT_SAMPLE_FACTORS           	C. violaceum	CV12472_liquid_6A_1	Sample source:Bacteria | Strain:QS+ | Culture medium:KMB_broth	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV12472_liquid_6A_1.smp
SUBJECT_SAMPLE_FACTORS           	C. vaccinii	CV328_solid_1C_1	Sample source:Bacteria | Strain:QS+ | Culture medium:KMB_agar	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV328_solid_1C_1.smp
SUBJECT_SAMPLE_FACTORS           	C. vaccinii	CV328w_solid_2A_1	Sample source:Bacteria | Strain:cviR- | Culture medium:KMB_agar	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV328w_solid_2A_1.smp
SUBJECT_SAMPLE_FACTORS           	C. vaccinii	CV328_solid_3C_1	Sample source:Bacteria | Strain:QS+ | Culture medium:KMB_agar	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV328_solid_3C_1.smp
SUBJECT_SAMPLE_FACTORS           	C. violaceum	CV12472w_liquid_6A_1	Sample source:Bacteria | Strain:cviR- | Culture medium:KMB_broth	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV12472w_liquid_6A_1.smp
SUBJECT_SAMPLE_FACTORS           	C. violaceum	CV12472_liquid_2C_1	Sample source:Bacteria | Strain:QS+ | Culture medium:KMB_broth	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV12472_liquid_2C_1.smp
SUBJECT_SAMPLE_FACTORS           	Media	KMB_solid_3C_1	Sample source:Blank | Strain:NA | Culture medium:KMB_agar	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=KMB_solid_3C_1.smp
SUBJECT_SAMPLE_FACTORS           	Media	KMB_liquid_1A_1	Sample source:Blank | Strain:NA | Culture medium:KMB_broth	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=KMB_liquid_1A_1.smp
SUBJECT_SAMPLE_FACTORS           	C. vaccinii	CV328_liquid_4C_1	Sample source:Bacteria | Strain:QS+ | Culture medium:KMB_broth	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV328_liquid_4C_1.smp
SUBJECT_SAMPLE_FACTORS           	C. vaccinii	CV328_solid_5B_1	Sample source:Bacteria | Strain:QS+ | Culture medium:KMB_agar	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV328_solid_5B_1.smp
SUBJECT_SAMPLE_FACTORS           	C. violaceum	CV12472w_solid_5A_1	Sample source:Bacteria | Strain:cviR- | Culture medium:KMB_agar	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV12472w_solid_5A_1.smp
SUBJECT_SAMPLE_FACTORS           	C. violaceum	CV12472w_solid_3B_1	Sample source:Bacteria | Strain:cviR- | Culture medium:KMB_agar	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV12472w_solid_3B_1.smp
SUBJECT_SAMPLE_FACTORS           	Media	KMB_solid_1A_1	Sample source:Blank | Strain:NA | Culture medium:KMB_agar	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=KMB_solid_1A_1.smp
SUBJECT_SAMPLE_FACTORS           	C. vaccinii	CV328w_liquid_6A_1	Sample source:Bacteria | Strain:cviR- | Culture medium:KMB_broth	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV328w_liquid_6A_1.smp
SUBJECT_SAMPLE_FACTORS           	C. vaccinii	CV328w_solid_5C_1	Sample source:Bacteria | Strain:cviR- | Culture medium:KMB_agar	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV328w_solid_5C_1.smp
SUBJECT_SAMPLE_FACTORS           	C. violaceum	CV12472_solid_3A_1	Sample source:Bacteria | Strain:QS+ | Culture medium:KMB_agar	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV12472_solid_3A_1.smp
SUBJECT_SAMPLE_FACTORS           	C. violaceum	CV12472w_liquid_4A_1	Sample source:Bacteria | Strain:cviR- | Culture medium:KMB_broth	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV12472w_liquid_4A_1.smp
SUBJECT_SAMPLE_FACTORS           	C. vaccinii	CV328w_solid_3A_1	Sample source:Bacteria | Strain:cviR- | Culture medium:KMB_agar	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV328w_solid_3A_1.smp
SUBJECT_SAMPLE_FACTORS           	C. vaccinii	CV328w_liquid_1B_1	Sample source:Bacteria | Strain:cviR- | Culture medium:KMB_broth	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV328w_liquid_1B_1.smp
SUBJECT_SAMPLE_FACTORS           	C. vaccinii	CV328_solid_6C_1	Sample source:Bacteria | Strain:QS+ | Culture medium:KMB_agar	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV328_solid_6C_1.smp
SUBJECT_SAMPLE_FACTORS           	C. vaccinii	CV328w_solid_2B_1	Sample source:Bacteria | Strain:cviR- | Culture medium:KMB_agar	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV328w_solid_2B_1.smp
SUBJECT_SAMPLE_FACTORS           	C. violaceum	CV12472w_solid_6C_1	Sample source:Bacteria | Strain:cviR- | Culture medium:KMB_agar	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV12472w_solid_6C_1.smp
SUBJECT_SAMPLE_FACTORS           	C. vaccinii	CV328w_solid_1A_1	Sample source:Bacteria | Strain:cviR- | Culture medium:KMB_agar	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV328w_solid_1A_1.smp
SUBJECT_SAMPLE_FACTORS           	C. vaccinii	CV328_solid_4C_1	Sample source:Bacteria | Strain:QS+ | Culture medium:KMB_agar	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV328_solid_4C_1.smp
SUBJECT_SAMPLE_FACTORS           	C. violaceum	CV12472w_liquid_3B_1	Sample source:Bacteria | Strain:cviR- | Culture medium:KMB_broth	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV12472w_liquid_3B_1.smp
SUBJECT_SAMPLE_FACTORS           	C. violaceum	CV12472_liquid_1A_1	Sample source:Bacteria | Strain:QS+ | Culture medium:KMB_broth	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV12472_liquid_1A_1.smp
SUBJECT_SAMPLE_FACTORS           	C. vaccinii	CV328_liquid_2B_1	Sample source:Bacteria | Strain:QS+ | Culture medium:KMB_broth	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV328_liquid_2B_1.smp
SUBJECT_SAMPLE_FACTORS           	C. vaccinii	CV328w_liquid_4A_1	Sample source:Bacteria | Strain:cviR- | Culture medium:KMB_broth	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV328w_liquid_4A_1.smp
SUBJECT_SAMPLE_FACTORS           	C. violaceum	CV12472_solid_5C_1	Sample source:Bacteria | Strain:QS+ | Culture medium:KMB_agar	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV12472_solid_5C_1.smp
SUBJECT_SAMPLE_FACTORS           	C. violaceum	CV12472_solid_4A_1	Sample source:Bacteria | Strain:QS+ | Culture medium:KMB_agar	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV12472_solid_4A_1.smp
SUBJECT_SAMPLE_FACTORS           	C. vaccinii	CV328w_solid_6C_1	Sample source:Bacteria | Strain:cviR- | Culture medium:KMB_agar	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV328w_solid_6C_1.smp
SUBJECT_SAMPLE_FACTORS           	C. vaccinii	CV328_liquid_5B_1	Sample source:Bacteria | Strain:QS+ | Culture medium:KMB_broth	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV328_liquid_5B_1.smp
SUBJECT_SAMPLE_FACTORS           	C. vaccinii	CV328_solid_6A_1	Sample source:Bacteria | Strain:QS+ | Culture medium:KMB_agar	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV328_solid_6A_1.smp
SUBJECT_SAMPLE_FACTORS           	C. vaccinii	CV328w_solid_1B_1	Sample source:Bacteria | Strain:cviR- | Culture medium:KMB_agar	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV328w_solid_1B_1.smp
SUBJECT_SAMPLE_FACTORS           	C. vaccinii	CV328w_solid_6A_1	Sample source:Bacteria | Strain:cviR- | Culture medium:KMB_agar	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV328w_solid_6A_1.smp
SUBJECT_SAMPLE_FACTORS           	Media	KMB_solid_1C_1	Sample source:Blank | Strain:NA | Culture medium:KMB_agar	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=KMB_solid_1C_1.smp
SUBJECT_SAMPLE_FACTORS           	C. violaceum	CV12472_liquid_5B_1	Sample source:Bacteria | Strain:QS+ | Culture medium:KMB_broth	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV12472_liquid_5B_1.smp
SUBJECT_SAMPLE_FACTORS           	C. violaceum	CV12472w_solid_1C_1	Sample source:Bacteria | Strain:cviR- | Culture medium:KMB_agar	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV12472w_solid_1C_1.smp
SUBJECT_SAMPLE_FACTORS           	C. vaccinii	CV328w_solid_3C_1	Sample source:Bacteria | Strain:cviR- | Culture medium:KMB_agar	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV328w_solid_3C_1.smp
SUBJECT_SAMPLE_FACTORS           	C. violaceum	CV12472_liquid_6B_1	Sample source:Bacteria | Strain:QS+ | Culture medium:KMB_broth	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV12472_liquid_6B_1.smp
SUBJECT_SAMPLE_FACTORS           	C. vaccinii	CV328_liquid_1C_1	Sample source:Bacteria | Strain:QS+ | Culture medium:KMB_broth	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV328_liquid_1C_1.smp
SUBJECT_SAMPLE_FACTORS           	C. violaceum	CV12472w_solid_3C_1	Sample source:Bacteria | Strain:cviR- | Culture medium:KMB_agar	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV12472w_solid_3C_1.smp
SUBJECT_SAMPLE_FACTORS           	Media	KMB_liquid_3C_1	Sample source:Blank | Strain:NA | Culture medium:KMB_broth	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=KMB_liquid_3C_1.smp
SUBJECT_SAMPLE_FACTORS           	C. violaceum	CV12472_liquid_6C_1	Sample source:Bacteria | Strain:QS+ | Culture medium:KMB_broth	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV12472_liquid_6C_1.smp
SUBJECT_SAMPLE_FACTORS           	C. vaccinii	CV328_solid_3A_1	Sample source:Bacteria | Strain:QS+ | Culture medium:KMB_agar	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV328_solid_3A_1.smp
SUBJECT_SAMPLE_FACTORS           	C. vaccinii	CV328w_liquid_3B_1	Sample source:Bacteria | Strain:cviR- | Culture medium:KMB_broth	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV328w_liquid_3B_1.smp
SUBJECT_SAMPLE_FACTORS           	C. violaceum	CV12472_solid_4C_1	Sample source:Bacteria | Strain:QS+ | Culture medium:KMB_agar	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV12472_solid_4C_1.smp
SUBJECT_SAMPLE_FACTORS           	C. vaccinii	CV328_liquid_3A_1	Sample source:Bacteria | Strain:QS+ | Culture medium:KMB_broth	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV328_liquid_3A_1.smp
SUBJECT_SAMPLE_FACTORS           	C. vaccinii	CV328_liquid_4B_1	Sample source:Bacteria | Strain:QS+ | Culture medium:KMB_broth	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV328_liquid_4B_1.smp
SUBJECT_SAMPLE_FACTORS           	Media	KMB_liquid_3B_1	Sample source:Blank | Strain:NA | Culture medium:KMB_broth	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=KMB_liquid_3B_1.smp
SUBJECT_SAMPLE_FACTORS           	C. vaccinii	CV328w_liquid_6C_1	Sample source:Bacteria | Strain:cviR- | Culture medium:KMB_broth	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV328w_liquid_6C_1.smp
SUBJECT_SAMPLE_FACTORS           	C. vaccinii	CV328_liquid_3B_1	Sample source:Bacteria | Strain:QS+ | Culture medium:KMB_broth	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV328_liquid_3B_1.smp
SUBJECT_SAMPLE_FACTORS           	C. violaceum	CV12472w_liquid_3C_1	Sample source:Bacteria | Strain:cviR- | Culture medium:KMB_broth	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV12472w_liquid_3C_1.smp
SUBJECT_SAMPLE_FACTORS           	Alkane Standard	KI Mix_4	Sample source:NA | Strain:NA | Culture medium:NA	Culture temperature=NA; RAW_FILE_NAME(Raw file name)=KI Mix_4.smp
SUBJECT_SAMPLE_FACTORS           	Empty run	Blank_13	Sample source:NA | Strain:NA | Culture medium:NA	Culture temperature=NA; RAW_FILE_NAME(Raw file name)=Blank_13.smp
SUBJECT_SAMPLE_FACTORS           	C. violaceum	CV12472_liquid_3C_1	Sample source:Bacteria | Strain:QS+ | Culture medium:KMB_broth	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV12472_liquid_3C_1.smp
SUBJECT_SAMPLE_FACTORS           	C. vaccinii	CV328_liquid_5C_1	Sample source:Bacteria | Strain:QS+ | Culture medium:KMB_broth	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV328_liquid_5C_1.smp
SUBJECT_SAMPLE_FACTORS           	C. violaceum	CV12472w_solid_6A_1	Sample source:Bacteria | Strain:cviR- | Culture medium:KMB_agar	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV12472w_solid_6A_1.smp
SUBJECT_SAMPLE_FACTORS           	C. violaceum	CV12472w_liquid_1C_1	Sample source:Bacteria | Strain:cviR- | Culture medium:KMB_broth	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV12472w_liquid_1C_1.smp
SUBJECT_SAMPLE_FACTORS           	C. vaccinii	CV328_liquid_6C_1	Sample source:Bacteria | Strain:QS+ | Culture medium:KMB_broth	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV328_liquid_6C_1.smp
SUBJECT_SAMPLE_FACTORS           	C. violaceum	CV12472w_liquid_5C_1	Sample source:Bacteria | Strain:cviR- | Culture medium:KMB_broth	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV12472w_liquid_5C_1.smp
SUBJECT_SAMPLE_FACTORS           	Media	KMB_solid_1B_1	Sample source:Blank | Strain:NA | Culture medium:KMB_agar	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=KMB_solid_1B_1.smp
SUBJECT_SAMPLE_FACTORS           	C. violaceum	CV12472w_solid_3A_1	Sample source:Bacteria | Strain:cviR- | Culture medium:KMB_agar	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV12472w_solid_3A_1.smp
SUBJECT_SAMPLE_FACTORS           	C. violaceum	CV12472_solid_5B_1	Sample source:Bacteria | Strain:QS+ | Culture medium:KMB_agar	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV12472_solid_5B_1.smp
SUBJECT_SAMPLE_FACTORS           	Media	KMB_liquid_2B_1	Sample source:Blank | Strain:NA | Culture medium:KMB_broth	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=KMB_liquid_2B_1.smp
SUBJECT_SAMPLE_FACTORS           	C. vaccinii	CV328_solid_5C_1	Sample source:Bacteria | Strain:QS+ | Culture medium:KMB_agar	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV328_solid_5C_1.smp
SUBJECT_SAMPLE_FACTORS           	C. vaccinii	CV328w_solid_3B_1	Sample source:Bacteria | Strain:cviR- | Culture medium:KMB_agar	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV328w_solid_3B_1.smp
SUBJECT_SAMPLE_FACTORS           	C. vaccinii	CV328_liquid_3C_1	Sample source:Bacteria | Strain:QS+ | Culture medium:KMB_broth	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=CV328_liquid_3C_1.smp
SUBJECT_SAMPLE_FACTORS           	Media	KMB_solid_3A_1	Sample source:Blank | Strain:NA | Culture medium:KMB_agar	Culture temperature=25°C; RAW_FILE_NAME(Raw file name)=KMB_solid_3A_1.smp
#COLLECTION
CO:COLLECTION_SUMMARY            	Quorum sensing sufficient (QS+) Chromobacterium violaceum (ATCC 12472),
CO:COLLECTION_SUMMARY            	Chromobacterium vaccinii (MWU 328), and their respective isogenic quorum sensing
CO:COLLECTION_SUMMARY            	mutants (cviR-), 12472w and 328w, were cultured in King’s Medium B (KMB). Six
CO:COLLECTION_SUMMARY            	biological replicates of each strain were prepared for each media type (i.e.,
CO:COLLECTION_SUMMARY            	broth or agar). Uninoculated medium controls were prepared in triplicates for
CO:COLLECTION_SUMMARY            	KMB broth and KMB agar, respectively.
CO:SAMPLE_TYPE                   	Bacterial cells
#TREATMENT
TR:TREATMENT_SUMMARY             	Four different strains of Chromobacterium (ATCC 12472, 12472w, MWU 328, 328w)
TR:TREATMENT_SUMMARY             	were inoculated in King’s Medium B (KMB) broth and incubated overnight at
TR:TREATMENT_SUMMARY             	25ºC. Sub-cultures of each strain were transferred into replicates of KMB broth
TR:TREATMENT_SUMMARY             	(liquid) and KMB agar (solid). Culture replicates were incubated at 25ºC for 7
TR:TREATMENT_SUMMARY             	h and 15.5 h for broth and agar, respectively.
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	The volatile metabolites were captured using headspace thin-film solid-phase
SP:SAMPLEPREP_SUMMARY            	microextraction (HS-TF-SPME). For volatile collection, thin-films composed of
SP:SAMPLEPREP_SUMMARY            	divinylbenzene and polydimethylsiloxane (DVB/PDMS) on a carbon mesh were placed
SP:SAMPLEPREP_SUMMARY            	in technical triplicates in each biological culture or media-only control. For
SP:SAMPLEPREP_SUMMARY            	broth cultures, thin-films were suspended in the flask headspace. For agar
SP:SAMPLEPREP_SUMMARY            	cultures, thin-films were placed on the inverted lid of the petri dish. Cultures
SP:SAMPLEPREP_SUMMARY            	were incubated at 25ºC for an additional 1 h for volatile collection. After VOC
SP:SAMPLEPREP_SUMMARY            	collection, thin-films were removed from cultures and place in thermal
SP:SAMPLEPREP_SUMMARY            	desorption tubes and stored at 4ºC prior to analysis. Volatile metabolite
SP:SAMPLEPREP_SUMMARY            	analysis was performed using a Gerstel® MPS Robotic Pro MultiPurpose
SP:SAMPLEPREP_SUMMARY            	autosampler directed by Maestro® software (Gerstel®, Inc., Linthicum, MD) and
SP:SAMPLEPREP_SUMMARY            	two-dimensional gas chromatography−time-of-flight mass spectrometry
SP:SAMPLEPREP_SUMMARY            	(GC×GC–TOFMS) using a LECO® Pegasus® 4D and Agilent® 7890B GC (LECO®
SP:SAMPLEPREP_SUMMARY            	Corp., St. Joseph, MI).
SP:EXTRACTION_METHOD             	Headspace thin-film microextraction (HS-TFME)
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_SUMMARY        	Column 1: Rxi-624Sil MS,(60m × 0.25mm × 1.4um); Column 2: Stabilwax,(1m ×
CH:CHROMATOGRAPHY_SUMMARY        	0.25mm × 0.5um)
CH:CHROMATOGRAPHY_TYPE           	GC
CH:INSTRUMENT_NAME               	Agilent 7890B
CH:COLUMN_NAME                   	Rxi-624Sil MS (60m × 0.25mm, 1.4um) and Stabilwax (1m × 0.25mm, 0.5um)
CH:SOLVENT_A                     	NA
CH:SOLVENT_B                     	NA
CH:FLOW_GRADIENT                 	NA
CH:FLOW_RATE                     	2 mL∙min-1 (constant)
CH:COLUMN_TEMPERATURE            	NA
CH:METHODS_FILENAME              	ST_GCxGC_methods.pdf
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
AN:ANALYSIS_PROTOCOL_FILE        	ST_MS_methods.pdf
#MS
MS:INSTRUMENT_NAME               	Leco Pegasus 4D GCxGC TOF
MS:INSTRUMENT_TYPE               	TOF
MS:MS_TYPE                       	EI
MS:ION_MODE                      	POSITIVE
MS:MS_COMMENTS                   	See attached file. RT1 and RT2 refer to the peak retention times for column 1
MS:MS_COMMENTS                   	and 2, respectively. Column 1: Rxi-624Sil MS,(60m × 0.25mm × 1.4um); 2:
MS:MS_COMMENTS                   	Stabilwax,(1m × 0.25mm × 0.5um)
MS:MS_RESULTS_FILE               	ST004091_AN006793_Results.txt	UNITS:Peak areas	Has m/z:No	Has RT:Yes	RT units:Seconds
#END