#METABOLOMICS WORKBENCH jcagmat_20250731_145838 DATATRACK_ID:6238 STUDY_ID:ST004118 ANALYSIS_ID:AN006829 PROJECT_ID:PR002572
VERSION             	1
CREATED_ON             	August 15, 2025, 3:33 pm
#PROJECT
PR:PROJECT_TITLE                 	Lipidomics Characterization of the PNPLA3(I148M) Neomorph in Hep3B Cells and
PR:PROJECT_TITLE                 	Plasma VLDL
PR:PROJECT_TYPE                  	Untargeted Lipidomics
PR:PROJECT_SUMMARY               	Lipidomics analysis reveals a central role in PNPLA3-I148M–associated fatty
PR:PROJECT_SUMMARY               	liver disease. Cells expressing the PNPLA3-I148M variant exhibit a distinct
PR:PROJECT_SUMMARY               	lipid profile characterized by an accumulation of polyunsaturated fatty acid
PR:PROJECT_SUMMARY               	(PUFA)-containing triglycerides, alongside a reduction in PUFA-containing
PR:PROJECT_SUMMARY               	phosphatidylcholine. This lipid imbalance leads to decreased membrane fluidity,
PR:PROJECT_SUMMARY               	which disrupts the biogenesis and secretion of very low-density lipoproteins
PR:PROJECT_SUMMARY               	(VLDLs). Untargeted lipidomics identifies a key mechanistic link between the
PR:PROJECT_SUMMARY               	altered lipid composition in I148M cells and impaired apolipoprotein B (ApoB)
PR:PROJECT_SUMMARY               	secretion. The defect in lipid processing and VLDL assembly can be partially
PR:PROJECT_SUMMARY               	reversed by overexpression of ABHD5/CGI-58, highlighting the critical role of
PR:PROJECT_SUMMARY               	CGI-58–dependent lipid remodeling pathways. These findings suggest that
PR:PROJECT_SUMMARY               	PNPLA3-I148M drives fatty liver disease through a neomorphic mechanism that
PR:PROJECT_SUMMARY               	disrupts cellular lipid homeostasis, particularly through alterations in PUFA
PR:PROJECT_SUMMARY               	distribution and impaired lipid secretion.
PR:INSTITUTE                     	Amgen
PR:LAST_NAME                     	Cagmat
PR:FIRST_NAME                    	Joy
PR:ADDRESS                       	750 Gateway Blvd, South San Francisco, CA 94080
PR:EMAIL                         	jcagmat@amgen.com
PR:PHONE                         	352-870-5378
#STUDY
ST:STUDY_TITLE                   	Lipidomics on Hep3B Cells Overexpressing CGI-158 and PNPLA2
ST:STUDY_TYPE                    	Untargeted Lipidomics
ST:STUDY_SUMMARY                 	We have found a decrease in PUFA-containing PC in I148M-expressing Hep3B cells,
ST:STUDY_SUMMARY                 	causing reduced membrane fluidity and therefore, impaired ApoB secretion. It
ST:STUDY_SUMMARY                 	appears that the PUFA’s are sequestered in triglycerides. We also previously
ST:STUDY_SUMMARY                 	found that overexpressing either CGI-58 or PNPLA2 in the I148M-expressing Hep3B
ST:STUDY_SUMMARY                 	cells rescues ApoB secretion. Therefore, we want to see how overexpression of
ST:STUDY_SUMMARY                 	either of these proteins affects the intracellular lipidome.
ST:INSTITUTE                     	Amgen
ST:LAST_NAME                     	Cagmat
ST:FIRST_NAME                    	Joy
ST:ADDRESS                       	750 Gateway Blvd
ST:EMAIL                         	jcagmat@amgen.com
ST:PHONE                         	352-870-5378
#SUBJECT
SU:SUBJECT_TYPE                  	Cultured cells
SU:SUBJECT_SPECIES               	Homo sapiens
SU:TAXONOMY_ID                   	9606
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data
SUBJECT_SAMPLE_FACTORS           	-	PNPLA3_followupexpt_1_Sample	Sample source:Hep3B Cells | Genotype:WT | Treatment:Control	RAW_FILE_NAME(Raw File Name)=PNPLA3_followupexpt_1_Sample.mzXML
SUBJECT_SAMPLE_FACTORS           	-	PNPLA3_followupexpt_2_Sample	Sample source:Hep3B Cells | Genotype:WT | Treatment:Control	RAW_FILE_NAME(Raw File Name)=PNPLA3_followupexpt_2_Sample.mzXML
SUBJECT_SAMPLE_FACTORS           	-	PNPLA3_followupexpt_3_Sample	Sample source:Hep3B Cells | Genotype:WT | Treatment:Control	RAW_FILE_NAME(Raw File Name)=PNPLA3_followupexpt_3_Sample.mzXML
SUBJECT_SAMPLE_FACTORS           	-	PNPLA3_followupexpt_4_Sample	Sample source:Hep3B Cells | Genotype:I148 | Treatment:Control	RAW_FILE_NAME(Raw File Name)=PNPLA3_followupexpt_4_Sample.mzXML
SUBJECT_SAMPLE_FACTORS           	-	PNPLA3_followupexpt_5_Sample	Sample source:Hep3B Cells | Genotype:I148 | Treatment:Control	RAW_FILE_NAME(Raw File Name)=PNPLA3_followupexpt_5_Sample.mzXML
SUBJECT_SAMPLE_FACTORS           	-	PNPLA3_followupexpt_6_Sample	Sample source:Hep3B Cells | Genotype:I148 | Treatment:Control	RAW_FILE_NAME(Raw File Name)=PNPLA3_followupexpt_6_Sample.mzXML
SUBJECT_SAMPLE_FACTORS           	-	PNPLA3_followupexpt_7_Sample	Sample source:Hep3B Cells | Genotype:WT | Treatment:CGI-58 overexpressed	RAW_FILE_NAME(Raw File Name)=PNPLA3_followupexpt_7_Sample.mzXML
SUBJECT_SAMPLE_FACTORS           	-	PNPLA3_followupexpt_8_Sample	Sample source:Hep3B Cells | Genotype:WT | Treatment:CGI-58 overexpressed	RAW_FILE_NAME(Raw File Name)=PNPLA3_followupexpt_8_Sample.mzXML
SUBJECT_SAMPLE_FACTORS           	-	PNPLA3_followupexpt_9_Sample	Sample source:Hep3B Cells | Genotype:WT | Treatment:CGI-58 overexpressed	RAW_FILE_NAME(Raw File Name)=PNPLA3_followupexpt_9_Sample.mzXML
SUBJECT_SAMPLE_FACTORS           	-	PNPLA3_followupexpt_10_Sample	Sample source:Hep3B Cells | Genotype:I148 | Treatment:CGI-58 overexpressed	RAW_FILE_NAME(Raw File Name)=PNPLA3_followupexpt_10_Sample.mzXML
SUBJECT_SAMPLE_FACTORS           	-	PNPLA3_followupexpt_11_Sample	Sample source:Hep3B Cells | Genotype:I148 | Treatment:CGI-58 overexpressed	RAW_FILE_NAME(Raw File Name)=PNPLA3_followupexpt_11_Sample.mzXML
SUBJECT_SAMPLE_FACTORS           	-	PNPLA3_followupexpt_12_Sample	Sample source:Hep3B Cells | Genotype:I148 | Treatment:CGI-58 overexpressed	RAW_FILE_NAME(Raw File Name)=PNPLA3_followupexpt_12_Sample.mzXML
SUBJECT_SAMPLE_FACTORS           	-	PNPLA3_followupexpt_13_Sample	Sample source:Hep3B Cells | Genotype:WT | Treatment:PNPLA2 overexpressed	RAW_FILE_NAME(Raw File Name)=PNPLA3_followupexpt_13_Sample.mzXML
SUBJECT_SAMPLE_FACTORS           	-	PNPLA3_followupexpt_14_Sample	Sample source:Hep3B Cells | Genotype:WT | Treatment:PNPLA2 overexpressed	RAW_FILE_NAME(Raw File Name)=PNPLA3_followupexpt_14_Sample.mzXML
SUBJECT_SAMPLE_FACTORS           	-	PNPLA3_followupexpt_15_Sample	Sample source:Hep3B Cells | Genotype:WT | Treatment:PNPLA2 overexpressed	RAW_FILE_NAME(Raw File Name)=PNPLA3_followupexpt_15_Sample.mzXML
SUBJECT_SAMPLE_FACTORS           	-	PNPLA3_followupexpt_16_Sample	Sample source:Hep3B Cells | Genotype:I148 | Treatment:PNPLA2 overexpressed	RAW_FILE_NAME(Raw File Name)=PNPLA3_followupexpt_16_Sample.mzXML
SUBJECT_SAMPLE_FACTORS           	-	PNPLA3_followupexpt_17_Sample	Sample source:Hep3B Cells | Genotype:I148 | Treatment:PNPLA2 overexpressed	RAW_FILE_NAME(Raw File Name)=PNPLA3_followupexpt_17_Sample.mzXML
SUBJECT_SAMPLE_FACTORS           	-	PNPLA3_followupexpt_18_Sample	Sample source:Hep3B Cells | Genotype:I148 | Treatment:PNPLA2 overexpressed	RAW_FILE_NAME(Raw File Name)=PNPLA3_followupexpt_18_Sample.mzXML
#COLLECTION
CO:COLLECTION_SUMMARY            	The generation of paired Hep3B cells (PNPLA3 WT and I148M) was previously
CO:COLLECTION_SUMMARY            	described elsewhere1. PNPLA3 knockout cells were generated by CRISPR gene
CO:COLLECTION_SUMMARY            	editing of parental Hep3B cells at Synthego (Redwood City, CA, USA).
CO:COLLECTION_SUMMARY            	Ribonucleoprotein complexes containing synthetic chemically-modified guide RNA
CO:COLLECTION_SUMMARY            	were electroporated into the cells using EditCo’s optimized protocol. Editing
CO:COLLECTION_SUMMARY            	efficiency was assessed upon recovery, 48 h post-electroporation. Genomic DNA
CO:COLLECTION_SUMMARY            	was extracted from a portion of the cells, PCR-amplified and sequenced using
CO:COLLECTION_SUMMARY            	Sanger sequencing. The resulting chromatograms were processed using EditCo’s
CO:COLLECTION_SUMMARY            	Inference of CRISPR Edits (ICE) software (ice.synthego.com). To create
CO:COLLECTION_SUMMARY            	monoclonal cell populations, edited cell pools were seeded at 1 cell/well using
CO:COLLECTION_SUMMARY            	a single-cell printer into 96- or 384-well plates. All wells were imaged every 3
CO:COLLECTION_SUMMARY            	days to ensure expansion from a single-cell clone. Clonal populations were
CO:COLLECTION_SUMMARY            	screened and identified using the PCR-Sanger-ICE genotyping strategy described
CO:COLLECTION_SUMMARY            	above. CGI-58- and PNPLA2-overexpressing cell lines were generated by
CO:COLLECTION_SUMMARY            	transducing Hep3B WT and I148M cells (250,000 cells/well of a 6-well plate) with
CO:COLLECTION_SUMMARY            	lentivirus, with a multiplicity of infection (MOI) of 10 and 10 µg/mL
CO:COLLECTION_SUMMARY            	polybrene. After 72 h, media was exchanged and cells were allowed to recover.
CO:COLLECTION_SUMMARY            	The following day, media was supplemented with 2 µM puromycin dihydrochloride
CO:COLLECTION_SUMMARY            	for selection. After 2 weeks of selection, cells were allowed to recover and
CO:COLLECTION_SUMMARY            	propagate for use in experiments. 1Sherman, D.J., Liu, L., Mamrosh, J.L., Xie,
CO:COLLECTION_SUMMARY            	J., Ferbas, J., Lomenick, B., Ladinsky, M.S., Verma, R., Rulifson, I.C., and
CO:COLLECTION_SUMMARY            	Deshaies, R.J. (2024). The fatty liver disease–causing protein PNPLA3-I148M
CO:COLLECTION_SUMMARY            	alters lipid droplet–Golgi dynamics. Proceedings of the National Academy of
CO:COLLECTION_SUMMARY            	Sciences 121, e2318619121.
CO:SAMPLE_TYPE                   	Hep3B hepatocellular cancer cell line
#TREATMENT
TR:TREATMENT_SUMMARY             	Six groups of Hep3B cells (WT, WT + CGI-58, WT + PNPLA2, I148M, I148M + CGI-58,
TR:TREATMENT_SUMMARY             	and I148M + PNPLA2) were treated with oleic acid (100 µM) for 24 h. Cells were
TR:TREATMENT_SUMMARY             	seeded at 1,000,000 cells/well (2 mL media) in 6-well plates (EMEM + 10% FBS).
TR:TREATMENT_SUMMARY             	The next day, media was replaced with 2 mL fresh media containing 100 µM oleic
TR:TREATMENT_SUMMARY             	acid (Sigma O3008). After 24 h, cells were washed with 1x with cold PBS, then
TR:TREATMENT_SUMMARY             	scraped into 300 µL ice cold 80% methanol (LCMS-grade Fisher Optima) and stored
TR:TREATMENT_SUMMARY             	in n glass vials at -70°C for lipidomic experiment.
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	Lipids were extracted by resuspending the cell pellets in 100 μL water and 225
SP:SAMPLEPREP_SUMMARY            	μL methanol, and the resulting suspension was transferred to a glass culture
SP:SAMPLEPREP_SUMMARY            	tube. 10 μL of lipidomics internal standard (SPLASH® LIPIDOMIX®; Avanti Polar
SP:SAMPLEPREP_SUMMARY            	Lipids, #330707) was spiked into each sample, followed by vortexing and addition
SP:SAMPLEPREP_SUMMARY            	of 750 μL methyl-tert-butyl ether (MTBE). Samples were incubated on ice for 20
SP:SAMPLEPREP_SUMMARY            	min, vortexed, and 188 μL water was added, followed by another round of
SP:SAMPLEPREP_SUMMARY            	vortexing. Samples were then centrifuged for 10 min at 3,200 x g and the top
SP:SAMPLEPREP_SUMMARY            	layer was transferred to a glass autosampler vial and evaporated under a stream
SP:SAMPLEPREP_SUMMARY            	of nitrogen gas. Dried samples were reconstituted in isopropanol and subjected
SP:SAMPLEPREP_SUMMARY            	to LC-MS for analysis.
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_TYPE           	Reversed phase
CH:INSTRUMENT_NAME               	Thermo Vanquish
CH:COLUMN_NAME                   	Thermo Accucore C30 (150 x 2.1mm,2.6um)
CH:SOLVENT_A                     	60% acetonitrile/40% water; 10 mM ammonium formate; 0.1% formic acid
CH:SOLVENT_B                     	90% isopropanol/8% acetonitrile/2% water; 10 mM ammonium formate; 0.1% formic
CH:SOLVENT_B                     	acid
CH:FLOW_GRADIENT                 	The initial mobile phase composition was 30% B and was increased to 43% B by 5
CH:FLOW_GRADIENT                 	min, 50% B by 5.1 min, 70% B by 14 min, 99% B by 21 min, then held at 99% B
CH:FLOW_GRADIENT                 	until 24 min, followed by column equilibration at initial mobile phase
CH:FLOW_GRADIENT                 	conditions for 6 minutes.
CH:FLOW_RATE                     	0.35 mL/min
CH:COLUMN_TEMPERATURE            	40
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
#MS
MS:INSTRUMENT_NAME               	Thermo Q Exactive HF-X Orbitrap
MS:INSTRUMENT_TYPE               	Orbitrap
MS:MS_TYPE                       	ESI
MS:ION_MODE                      	NEGATIVE
MS:MS_COMMENTS                   	Full MS and ddMS2 was acquired from pooled sample; data processing and feature
MS:MS_COMMENTS                   	assignment was done by LipidMatch.
#MS_METABOLITE_DATA
MS_METABOLITE_DATA:UNITS	peak area
MS_METABOLITE_DATA_START
Samples	PNPLA3_followupexpt_1_Sample	PNPLA3_followupexpt_2_Sample	PNPLA3_followupexpt_3_Sample	PNPLA3_followupexpt_4_Sample	PNPLA3_followupexpt_5_Sample	PNPLA3_followupexpt_6_Sample	PNPLA3_followupexpt_7_Sample	PNPLA3_followupexpt_8_Sample	PNPLA3_followupexpt_9_Sample	PNPLA3_followupexpt_10_Sample	PNPLA3_followupexpt_11_Sample	PNPLA3_followupexpt_12_Sample	PNPLA3_followupexpt_13_Sample	PNPLA3_followupexpt_14_Sample	PNPLA3_followupexpt_15_Sample	PNPLA3_followupexpt_16_Sample	PNPLA3_followupexpt_17_Sample	PNPLA3_followupexpt_18_Sample
Factors	Sample source:Hep3B Cells | Genotype:WT | Treatment:Control	Sample source:Hep3B Cells | Genotype:WT | Treatment:Control	Sample source:Hep3B Cells | Genotype:WT | Treatment:Control	Sample source:Hep3B Cells | Genotype:I148 | Treatment:Control	Sample source:Hep3B Cells | Genotype:I148 | Treatment:Control	Sample source:Hep3B Cells | Genotype:I148 | Treatment:Control	Sample source:Hep3B Cells | Genotype:WT | Treatment:CGI-58 overexpressed	Sample source:Hep3B Cells | Genotype:WT | Treatment:CGI-58 overexpressed	Sample source:Hep3B Cells | Genotype:WT | Treatment:CGI-58 overexpressed	Sample source:Hep3B Cells | Genotype:I148 | Treatment:CGI-58 overexpressed	Sample source:Hep3B Cells | Genotype:I148 | Treatment:CGI-58 overexpressed	Sample source:Hep3B Cells | Genotype:I148 | Treatment:CGI-58 overexpressed	Sample source:Hep3B Cells | Genotype:WT | Treatment:PNPLA2 overexpressed	Sample source:Hep3B Cells | Genotype:WT | Treatment:PNPLA2 overexpressed	Sample source:Hep3B Cells | Genotype:WT | Treatment:PNPLA2 overexpressed	Sample source:Hep3B Cells | Genotype:I148 | Treatment:PNPLA2 overexpressed	Sample source:Hep3B Cells | Genotype:I148 | Treatment:PNPLA2 overexpressed	Sample source:Hep3B Cells | Genotype:I148 | Treatment:PNPLA2 overexpressed
Cer-NS(d18:2/24:1)	13251724.92	8302149.608	7733374.142	6668408.872	6301598.465	19014798.15	11169448.85	7102666.128	7628547.785	7569915.401	8085964.517	5095811.1	7460130.869	3693869.793	4757640.605	6054278.103	6757359.902	13194708.61
Cer-NS(d18:1/22:0)	5788919.782	3400887.197	3181504.737	2749735.376	2984236.569	7785582.146	4876453.19	3204028.989	3468442.646	3405067.786	3946615.292	2558278.537	3540233.962	1340031.234	1836148.026	2329236.825	3180485.739	5955144.426
Cer-NS(d18:2/16:0)	4007763.578	2826453.371	2717086.053	2131527.943	2454242.441	6106482.371	3999806.214	3291439.497	3453539.625	3153336.721	3518591.78	2140680.878	2991151.821	1350706.193	1694795.893	2533006.652	2084403.88	4176095.865
Cer-NS(d18:1/16:0)	3969932.595	2786830.807	2569307.949	2217462.145	2063269.701	5822228.803	3706995.012	2532209.866	2790645.308	2544280.569	2655629.569	1588725.73	2348366.532	1216373.419	1497103.926	1998449.196	2098196.371	4488876.158
Cer-NS(d18:1/24:1)	2140221.232	1239614.302	1180187.298	1066236.057	981556.8443	2982836.795	1914897.238	1268855.134	1348622.126	1409718.215	1563273.521	873653.1458	1275195.316	377959.8033	611592.3425	859680.7183	1005310.691	2406437.526
DMPE(14:0_16:0)	4256550.301	1980798.599	1939636.338	1600511.625	1843807.173	4898124.18	3363858.154	1085232.284	1261166.493	896900.7087	1161257.398	845522.5135	589134.5107	1021129.74	1050606.159	1964992.563	2132572.453	5574917.234
DMPE(16:0_18:1)	24975299.84	19155058.25	18681702.97	17087173.95	15601894.75	31184601.48	25193613.04	16235036.72	18430720.93	16921203.44	18146669.04	14149275.53	16673125.28	8863894.527	11078487.02	15497926.43	15180150.46	29972335.38
DMPE(16:0_16:1)	8517762.314	4468518.408	3453407.496	3618839.728	5230357.977	11410201.76	8789614.495	4967023.555	5261440.015	4683563.348	5050018.812	3718700.936	4111181.98	1689209.028	2432365.939	3554270.891	5083259.673	10192149.21
DMPE(16:0_16:0)	7082021.451	2988186.458	2807471.86	2608808.638	3131699.297	7160018.361	6258604.201	2906182.163	3423784.229	2851863.93	3774453.615	3168727.387	3930572.759	1592324.576	1828508.981	3040403.198	3272944.22	7138573.243
DMPE(16:1_18:1)	2447357.767	2249179.737	2262245.973	1966038.408	1331540.401	4736868.935	3226760.335	2509753.563	2733551.878	2169885.941	2875528.883	2020156.118	2595943.897	840018.4668	1030817.039	1516315.467	904081.0121	2974141.912
DMPE(18:1_20:1)	3343996.811	3350657.675	3310816.96	3174345.146	1638955.511	5318990.651	3442645.213	8415543.507	9495527.456	9139103.095	5541641.582	3435464.081	4964385.446	1633213.467	1975084.286	2556992.31	1320455.986	3332594.17
LPC(16:0)	6522963.566	3352362.949	3909633.41	3692868.505	5984659.599	6854161.359	8017955.868	3680300.423	4762813.871	3702965.207	4087976.309	4355193.958	3825710.416	2988759.588	2726377.403	3287862.652	5282469.923	8311703.599
LPC(18:0)	4921226.224	2024271.799	2581620.683	1974422.389	3090377.094	4588256.967	4262370.279	2707733.17	3446916.825	2044374.807	3912855.405	2795975.967	2831726.682	1415516.411	1663881.38	2096270.214	2964916.108	4941381.048
LPC(18:1)	363183.4077	357101.5614	506367.8414	493327.9885	612879.2997	577256.7722	722607.76	645146.1575	806945.3346	383835.4936	695953.7796	513830.0744	386623.3979	298853.6519	357413.5176	397156.5964	506489.7915	529459.5191
LPE(16:0)	1690308.397	694119.9982	522831.9243	396172.6425	1050620.065	1552542.601	1597193.33	483228.2452	552065.8977	364559.8152	290657.8123	397178.7012	242720.3453	343196.5027	237350.1246	451536.3829	894872.3963	1701125.355
LPE(18:0)	3849580.945	1546853.15	2043818.225	1548966.875	2563691.605	4301379.68	3775743.368	1941972.242	2241553.085	1649758.71	1812201.309	1364033.901	1502504.178	1009213.825	1109938.467	1612180.76	2666310.874	4156700.844
LPE(18:1)	4045781.024	2486767.33	2537584.756	2038130.945	2664321.109	3627339.013	3826537.988	2805774.006	3325055.458	2278301.052	1802365.207	1364323.205	1199293.889	986428.3147	1297424.868	1709114.455	2761857.348	3987042.728
PC(14:0_16:0)	107105404.8	47413247.23	47369651.12	40072775.74	49947187.03	112566069.9	83557257.77	29500908.04	34210631.17	29791284.54	27134632.87	21010005.73	23357618.15	27254095.87	33133762.06	50372090.65	60688776.14	121245240.7
PC(14:0_16:1)	9807686.853	5199399.567	7257000.78	4034289.494	4910331.279	10945443.74	8317201.294	4008376.762	5990770.946	4998530.399	3925756.329	3561162.213	2847514.104	2622816.313	3503083.651	5952584.921	5057261.82	10417763.02
PC(14:0_14:0)	4851545.593	2106730.193	1816997.878	1549412.954	1678092.459	4137535.337	3180610.748	624616.8061	770885.7183	523587.6196	319100.28	297131.1897	414634.128	937880.3292	1143032.962	2070290.206	2536267.738	5371733.84
PC(15:0_16:1)	1225490.543	451412.7839	484833.3198	601864.7835	341925.7091	1587839.526	1204553.597	686153.2125	661132.511	336152.9994	575789.4229	411782.7401	378284.058	222125.5563	315964.4453	566332.7288	647005.7098	1468607.132
PC(16:0_18:1)	808325666.7	613238708.5	603175642.2	555203201.8	511473798.7	1038296231	809367543.8	542538184.8	592424180.2	558121317.3	589760035.2	452288904.2	550177609	295383681.5	358000889.5	491334595.1	493331523.2	952075287.6
PC(16:0_16:1)	235021603.4	119856171.2	121320952.2	100927689.6	147247646.4	312176336.5	238958658.9	136228106.7	145710371.3	133474896.6	138204980.2	105118145.6	122596462.5	52470610.33	64667915.83	99928792.5	143769831.4	272677948.8
PC(16:0_16:0)	199347678	97227012.18	92162099.09	85262704.5	114337121.5	242546265.4	183760545.3	96858746.66	108067097.3	99427665.73	131043416.7	98824523.73	119522287.7	54625857.13	66233698.14	90744645.93	112316776.1	226723283.5
PC(16:0_20:4)	71702343.43	47675104.9	49565212.97	40405152.36	46806811.44	98093379.38	75697408.81	44716522.21	47461938.45	43947446.49	55339837.37	40998910.52	47453112.22	18443353.13	23289875.07	34946963.04	40891900	82660002.74
PC(16:0_20:1)	25901971.62	26477397.45	27096896.97	25135150.85	12756566.32	38642493.15	22426014.89	21707044.89	25601204.12	24703260.39	19916396.11	13604491.36	18282393.45	12724934.48	15682663.51	20259387.61	10454864.89	25578731.67
PC(16:0_18:0)	15930496.58	9598922.234	9471344.201	8113126.293	7502070.046	22230242.69	14268868.42	7986491.361	9377975.034	8356835.162	12112726.04	8172227.991	10784984.15	4573271.617	5782181.399	6963151.457	7335270.422	17515247.54
PC(16:0_22:6)	8969009.138	6196122.926	6402268.854	5260383.431	4483149.407	12366389.34	8220149.523	5375408.704	5767868.99	5314850.908	7129832.179	5376983.875	6824894.069	2129088.276	2926391.923	4353755.053	4511534.211	10112058.17
PC(16:0_20:5)	9876732.394	3868100.124	4164539.685	2950627.528	5732573.886	12976015.42	9393685.643	4106170.892	4457679.123	3984765.051	5296291.704	4182949.224	4076066.767	1010362.153	1440276.065	2248415.668	5073212.478	11638067.24
PC(16:0_22:4)	4138586.604	2309636.373	1703615.175	1825446.922	1823287.72	5699288.277	3141786.126	1982980.418	2489839.684	2594173.095	2641009.349	1900900.809	2410816.948	821272.568	844400.6219	2062287.168	1869015.615	4243040.542
PC(16:0_22:5)	3541970.063	1688748.441	1688408.735	916163.1943	1931420.297	5193053.686	3361347.395	1809427.074	2113802.885	1786990.33	2608740.005	1849544.807	2222149.956	810047.8337	1031746.983	871538.8845	1904809.53	4370474.315
PC(16:0_20:3)	1876265.664	844817.2468	990923.2778	733510.7863	1007136.858	2765624.545	1876427.258	852822.2727	570413.5256	794168.0524	953050.8073	439427.7919	778588.2933	403202.5221	221698.9676	643108.1011	921663.7742	2203368.2
PC(16:1_18:1)	169734767.6	110486554.8	159094068.4	135555595.4	132638855.7	233222618.8	111630289.6	167914635.6	178111345.6	169083325.1	196806298.5	133411242.7	161129122.5	58590760.66	72705102.89	103651342.1	84927905.5	177333293.2
PC(16:1_16:1)	8710646.845	6049941.923	4532833.596	4874777.618	6260248.36	13237630.54	9150027.72	9596420.138	10238435.45	9003257.062	11101405.32	8319599.225	8533278.288	2205645.48	2355090.117	3891897.939	4644988.805	9128426.388
PC(16:1_20:4)	1740593.276	1421898.992	1265762.59	1092614.876	1895939.072	2570570.491	1563972.418	1803458.043	1976917.039	1671389.64	2339941.781	1499015.74	2457595.632	813021.3282	1053355.654	569875.6095	307980.6351	1908994.951
PC(16:1_18:2)	1974469.674	976479.4441	1291178.638	754133.7175	612941.6816	3957558.865	1868976.638	1488159.041	1830123.036	1504263.612	3996060.547	2109449.568	2530080	606049.7398	818463.016	1282061.263	1464198.064	1961674.227
PC(16:1_17:1)	1507084.898	528310.74	1104299.411	959079.2617	1066585.91	2268286.276	1640433.89	416279.9653	1243736.429	1296979.709	1300347.471	1090864.441	1132724.358	237011.0308	387289.262	716085.9643	453818.7412	1813575.219
PC(17:0_18:1)	1819814.728	1179624.289	1526311.757	963494.8859	555864.2484	2010719.357	2006596.026	1372583.536	1581197.408	1108022.854	1500027.92	864523.6867	844838.2211	341646.0406	793853.8605	1126263.247	1071778.192	2083112.038
PC(18:0_18:1)	75546664.62	36881744.83	36048570.05	31855108.13	38473284.9	109170796	71640662.74	39382496.81	45875352.59	40914555.32	51644017.09	37094420.83	48284441.43	19232311.23	24461169.25	31413206.99	39194876.36	90779230.34
PC(18:0_18:2)	35614932.41	23646085.23	24655947.7	21193632.16	21786285.49	54481613.24	37016922.28	24453062.91	26869074.96	25521461.12	29519636.51	20880720.88	27447727.64	10799408.71	14089883.55	18874912.54	19866506.44	41667263.72
PC(18:0_20:3)	5829787.756	2786094.705	2986215.285	2392534.64	2478291.353	8574708.702	5025486.714	3153512.364	3981781.211	3198789.103	4086664.832	2707204.39	3543078.145	1316896.086	1805790.314	2706674.964	2663750.999	6881322.853
PC(18:0_22:6)	2228922.505	1130895.974	1325451.142	1229086.444	1140831.084	2732586.357	1863780.705	1476113.612	1528477.554	1254658.67	1898446.953	1305165.772	1586845.637	567727.9875	790969.7848	1138933.411	1032397.678	2407947.655
PC(18:0_22:4)	1794720.183	732050.0573	867211.7356	800021.396	755148.0657	2165005.253	1400427.015	837899.5484	1016881.509	947293.713	1312678.889	842215.8977	1162794.513	288730.0315	486426.803	762078.6276	684881.0113	1596448.211
PC(18:1_18:1)	336529511.9	322441211.7	324910542.7	306508864.7	212141587.3	471231248.7	345103037.3	329541913	361934960.4	353000963.8	418969922.9	277236297.5	373471199.2	146826008.7	180863799.1	230778581.5	186284896.9	387474120
PC(18:1_18:2)	52584899.34	41926273.92	42725888.41	36947856.89	35948348.77	84328807.99	59483877	44920792.25	48014175.44	44363535.72	53900339.68	35928524.95	44570968.27	14288549.04	18389951.47	26789445.46	27534449.41	56895389.04
PC(18:1_20:4)	45943559.87	39290863.34	40097163.99	34744158.2	30493436.73	69175837.87	50622443.84	43187850.09	44740985.53	42904385.41	60401425.95	39335258	49505936	14825036.74	18750607.83	26151902.71	23603450.41	51728573.03
PC(18:1_20:1)	33933728.66	27967428.74	29194903.37	26200900.79	16768041.29	51941818.3	31863488.33	34485447.92	39879517.55	38853135.97	38248875.51	23518821.15	35095137.36	13709206.39	16524598.53	21587818.71	15018145.89	37781312.21
PC(18:1_22:6)	3416692.699	3795263.927	3847846.054	2960996.981	2159035.796	5422624.418	3611075.735	3554318.697	3837725.739	3465307.207	5053576.023	2944874.354	4261538.424	1300777.901	1715222.142	2470748.993	1605272.795	4091676.156
PC(18:1_20:5)	5706325.152	2696916.305	3018578.582	2393759.347	3441751.593	7894644.248	5720427.975	3472395.558	3616531.632	3418378.562	4943015.38	3169946.87	4080873.481	672038.085	989221.5235	1649409.621	2793197.597	6461402.565
PC(18:1_20:2)	3238433.246	2147805.139	2493448.777	1705779.698	1461541.63	4615007.204	3174783.021	2291493.514	2042431.167	2230002.63	2278232.455	1342618.66	1734388.089	852580.9696	1657110.713	2003847.79	1618898.349	4247246.202
PC(18:1_22:1)	1308539.021	801643.5282	956329.7334	703250.1569	437288.6084	2264638.882	1333598.915	1502885.36	1455937.205	1793526.898	1598779.298	777982.5389	1406830.888	392454.3772	521401.2426	753126.0204	238318.1632	1554094.106
PC(18:2_20:1)	6333432.059	5058134.235	5875920.7	3967488.297	4603246.829	12016128.2	8341448.596	6294951.112	7329086.183	6726147.013	7391403.086	5260333.715	6796347.863	1936175.232	2189805.853	4966480.437	4286379.75	9168227.032
PC(20:1_20:4)	3935137.81	3478479.43	3454868.91	2934548.888	2472974.778	6423938.792	5904231.321	4292491.663	4285345.256	4856778.09	4737047.278	3092250.042	3899895.056	1521494.375	1861479.153	2964068.013	1959507.039	5026066.461
PE(16:0_18:1)	124469721	86714860.38	83792011.58	74495665.44	61952997.67	159366897.5	111613743.8	84182306.02	94314464.04	89311254.07	72997864.66	51389824.47	68242633.49	37383631.13	46695535.25	68152556.03	68042405.46	135663383.8
PE(16:0_20:4)	120744817.4	65281284.02	64079201.79	54147369.13	64834659.38	148830726.4	107515992.3	56493731.32	59848155.53	58618349.38	66701344.17	46507305.05	61045139.93	23839164.73	29546093.93	44878486.79	68255807.1	135397810.7
PE(16:0_20:3)	11630004.5	10128538.42	8339747.533	6736793.431	7440151.962	16680777.79	11162406.4	8227612.486	10752842.96	9913123.74	8088078.734	5687290.165	7573869.559	2930761.625	3680079.603	5911420.723	7973901.933	16069534.2
PE(16:0_20:5)	6838493.385	2875848.093	2964534.447	2272814.594	3560543.392	8981096.923	6295610.897	2721161.516	2920658.43	2633804.928	3054793.697	2310667.149	2781475.968	687176.4955	1049826.711	1632906.559	3816088.771	7805196.429
PE(16:0_18:0)	1451456.591	1156235.955	1062567.39	988489.4619	642436.0935	2161900.185	1280007.369	1067451.007	1323731.151	1302203.302	1239750.74	690299.2434	870822.8683	340498.2542	645669.2608	938933.3051	567692.9481	1437204.438
PE(16:1_18:1)	37710504.19	25195898.12	9299478.166	9055069.823	22048944.29	51383076.37	37133467.8	27417943.82	27939721.75	28060280.1	11030364.47	17933231.51	9612968.288	9698518.375	11613687.65	17597087.12	22540827.19	42338482.05
PE(16:1_18:0)	9191308.454	5424080.633	5329424.647	4785612.58	4828415.29	12751552.03	9551416.222	6491902.649	7513229.484	7212132.954	5893144.449	4096789.685	5804139.735	2827976.792	3617788.686	5453375.121	5077556.601	10337616.1
PE(16:1_20:4)	4289072.683	2152177.911	2232926.493	1668896.609	2444414.687	6267022.177	4223640.163	2348015.444	2700649.155	2576305.732	1815589.744	1492184.695	1607393.482	631533.8236	469076.5961	954614.6972	2659006.283	5024482.501
PE(16:1_22:6)	1065601.172	751391.6882	673947.8519	615218.866	403788.0969	1466508.118	923416.8223	610151.3364	539986.1461	582887.107	420625.3266	383845.6247	267900.6447	220034.6338	280719.6009	346168.6498	517466.8008	1263378.543
PE(16:1_18:2)	1098993.109	452628.9837	468261.3354	423109.4848	569376.327	1601217.594	979607.8147	516847.8804	587297.3585	409748.2195	239900.965	176790.1333	315995.7814	73893.68159	136796.0611	183900.8227	528214.3911	1284772.153
PE(17:0_20:4)	7939378.819	4738662.168	4541562.566	3872082.113	4156697.039	11431765.74	7540830.001	5080162.464	5340093.33	5352573.851	6730991.063	4535591.869	6168369.405	1746753.93	2242034.682	3406819.985	3846952.884	8600920.329
PE(18:0_20:4)	443482244.9	245036671.7	241927291.7	210033842.3	237988479.4	580966357.3	403325748.8	227490573.4	248497470.3	245788142.1	301047047.6	208706275.7	276335533.6	121283826.3	155286125.5	223079557.6	242666923.2	495843226.2
PE(18:0_18:2)	40619880.4	19387780.47	19110951.16	18020976.91	21656951.42	58702361.49	38204013.54	20926577.52	23002396.7	21641438.2	23418715.63	17081178.24	22204366.94	9017900.246	11692737.93	19074130.24	24523291.62	45277754.11
PE(18:0_22:6)	23378977.69	15710794.01	15334116.42	13414644.54	11143760.45	30831965.46	20619043.58	14255656.9	15676908.48	15531313.18	17221900.25	11667859.5	15788823.26	7918579.441	10253233.23	14956851.05	11528916.7	26054402.83
PE(18:0_20:3)	17761503.22	13022720.09	12917460.54	10870354.63	8870085.266	25051307.34	16101571.04	12905338.45	14042121.21	14045090.24	11537023.89	7897867.714	10662811.31	6560149.456	8302020.347	11014762.95	8828113.443	19303750.92
PE(18:0_20:5)	17390956.2	7501538.452	6989999.446	5866807.378	8625502.924	23019099.23	15424374.94	7233797.434	8416166.969	7547283.97	9485436.805	6899607.745	8673575.477	3036305.26	3918793.166	6224026.733	9234996.304	19100735.03
PE(18:0_22:5)	6396041.481	4601931.502	4032820.618	4260753.919	3368135.981	9714672.969	6284953.088	5313686.494	5288966.977	5316863.729	5786779.486	4097486.721	5952381.878	2200533.259	2586160.121	4387683.962	3188544.203	7257969.24
PE(18:0_22:4)	8333394.412	4668667.632	4390646.064	3773487.073	3706183.478	10884411.3	7074404.489	4272210.3	4647150.383	4785418.498	5550114.162	3447111.45	4958776.1	2441055.188	3149890.194	4231007.205	3835815.727	8698549.957
PE(18:0_20:2)	7155626.504	4708063.488	4387078.926	3934550.111	3520865.876	9159195.929	6138150.375	5298573.12	5918182.801	5573034.28	3581500.016	2169851.515	3342712.379	3210276.568	4029813.301	5009751.114	3792149.403	7598342.919
PE(18:0_20:1)	5465718.195	2965231.05	2678785.774	2399597.864	1969176.084	7283874.315	4017094.858	6156696.813	6811834.629	6522669.972	4205537.476	2838586.076	3988686.916	1808038.402	2360112.213	3087616.208	2204362.468	5010065.963
PE(18:0_22:2)	1363079.902	885760.5713	793336.1388	718274.3501	664559.0526	1963111.496	1146503.087	1003643.66	1105995.977	1086703.619	796018.9857	519192.0645	697992.7352	517633.7998	654665.6732	853524.7466	768962.5103	1570069.283
PE(18:1_18:1)	329423666.9	298922987.4	284592853.8	266715933.7	179085547.5	470194271.5	327560813.9	333459705.9	370300648.5	363726539.5	311085403.6	207873521.6	291363352.8	128628550.7	158694399.5	221490041.3	171372287.9	355358270.9
PE(18:1_20:4)	401683649	286007054.5	262310248	227671677.3	236942222.6	585415356.3	396975976.2	294888241.8	298825444.5	293217923.8	281872580.3	191809919.1	264318911.7	99380738.31	126325396.2	184581429.1	231509455.6	443883209.7
PE(18:1_18:2)	85748862.55	53058472.78	51528496.41	43159151.85	52439518.88	125193834.5	89740854.84	61073957.49	67535775.9	64982605.54	51934768.68	38978894.22	47898099.42	18966392.96	23216931.7	35224607.4	50038296.93	96323681.73
PE(18:1_22:6)	58323203.25	54924290.66	54577610.65	48954625.22	32967000.7	79035058.21	56738078.46	57076708.63	61494846.77	61361591.16	55385969.75	35406751.3	49652538.05	22886821.48	28723694.71	40236544.85	30592372.57	63320890.3
PE(18:1_20:1)	35271194.44	32927110.09	29919990.6	28007355.72	16053944.82	54733386.92	36223112.29	59640147.41	68097194.24	64880378.64	40059542.57	25772004.26	36045851.38	14853017.36	19001120.86	25980712.67	16825953.57	38425414.44
PE(18:1_20:3)	29685584.57	24638346.67	22903892.61	19974007.13	13029658.18	43385305.21	29179911.94	25084262.88	27062520.95	25524978.85	15799976.21	11338729.05	14231371.25	8398516.959	9018680.754	16916503.22	12822696.12	32120411.46
PE(18:1_20:5)	25401617.66	12645164.6	12458496.09	9726417.213	14254630.59	35727360.45	24096296.82	15093590.49	17087100.35	15925988.76	13363992.24	10285369.39	12358372.73	3502101.671	4769260.167	7677101.21	14302130.83	29093641.03
PE(18:1_22:5)	9272501.717	8059923.008	7866442.418	6771715.736	4392727.794	13435729.44	8665073.239	8209020.706	9374779.944	9115574.461	6674851.627	4296885.953	6018197.494	3250598.821	4230337.453	5959412.822	4318684.589	9619666.868
PE(18:1_20:2)	6840054.071	4755680.908	4455943.605	3815341.914	3195097.847	9750338.499	6245351.037	5691336.961	6356155.664	6084967.746	3094317.835	2323255.651	2792604.113	2718928.618	3327210.59	5112950.168	3683690.886	7435325.372
PE(18:1_22:2)	1672793.368	916773.7684	837684.0654	809613.4128	888742.2489	3167504.178	1656405.722	1804584.667	1924800.818	1980579.313	832216.2779	622967.24	1059834.567	415130.457	636495.1394	913626.3857	832916.9377	1846565.427
PE(19:0_20:4)	1234590.66	692135.9039	685813.4795	513403.35	523891.8717	1868249.15	1122953.806	697108.1514	752363.9985	792524.7251	885417.7836	572427.2736	805250.5642	267960.9437	303363.3647	479525.8971	501781.1483	1387341.899
PE(20:0_20:4)	2448037.034	1364215.487	1285782.808	1139058.487	1085666.934	3227111.434	2105275.042	1000036.285	1124530.614	1094405.171	1381660.484	798224.2321	1297794.454	415222.3652	473719.0027	915098.4151	1141597.138	2659568.285
PE(20:1_20:4)	24725156.78	20649256.55	20296772.03	17674241.32	12636603.31	34497213.63	22851758.82	23544078.42	25987513.92	25501781.35	22974100.57	14865754.45	20918836.23	9661547.031	11994403.65	17449842.96	12715689.53	27858606.89
PE(20:1_22:6)	2920313.754	3547377.046	3528071.436	3251761.959	1599437.221	4109441.359	2913234.617	4164301.783	4575428.402	4610409.715	3232535.458	2327903.375	3009579.829	1955791.89	2394458.942	3463029.397	1410426.93	2950703.028
PE(20:1_20:3)	2858064.412	2360969.438	2277885.294	1950304.143	1406649.759	3871698.163	2600853.458	2664050.976	2899461.991	2907132.321	2079098.259	1385662.178	1752806.665	1195763.946	1530758.214	1984431.424	862496.2168	3171678.922
PE(20:4_22:1)	4347275.303	3720008.793	3254974.544	2828891.08	2313279.788	7710131.299	4144856.163	3416789.262	3842259.765	4090177.559	4010498.133	2535348.644	3747572.767	1124071.331	1560160.397	2351317.617	2070730.878	5630047.736
PE(20:4_22:0)	1407307.591	832891.5426	707051.2841	621176.4043	622332.687	2087453.915	1349893.785	549972.1334	675458.2002	477764.5484	954213.4612	564753.6942	857268.1325	207375.4113	316301.1858	291521.7895	615015.6326	1528481.19
PE(20:4_24:1)	813580.6478	616447.2315	623083.7505	485731.5822	367713.0325	1238133.731	695910.5753	408683.2177	361554.4462	387576.005	429139.7304	247173.0817	330738.3801	175485.0017	213847.0881	386181.4295	441554.1343	858290.7998
PG(16:0_18:1)	16488249.29	8506044.052	8270760.3	7826397.979	7917212.432	17199381.22	11920965.54	8289143.26	9193020.445	9077683.395	7983805.905	5599907.047	7322909.563	4959121.628	5370874.905	8321521.847	8530688.724	16433787.36
PG(16:1_18:1)	4594073.171	1612745.03	1506808.113	1357761.867	4239618.444	7160768.065	6565694.452	2642587.737	3105781.235	2834075.518	3296110.755	2347036.823	3672156.868	422853.1566	519263.8336	963954.4913	2877783.67	5516188.735
PG(17:1_18:1)	1313081.503	440761.2914	442249.8781	344640.5216	1016319.658	2266864.054	1512817.598	408625.3142	260299.1455	281560.0711	372536.0057	210834.4859	274365.088	63079.88187	95148.0745	257858.4032	539275.5292	1328792.057
PG(18:0_18:1)	1779980.389	845816.2494	837974.4252	787387.5544	1387422.741	3668353.189	931125.097	864763.5453	1178016.603	1136619.29	978316.3136	476335.6789	1025317.561	618166.7082	414529.7452	803483.5308	487876.4674	2700968.559
PG(18:1_18:1)	148592281.6	58689440.33	60024428.73	48042049.13	89350758.36	237505014.6	157652667.5	62723400.46	70175456.22	60238918.09	69957855.68	45756185.94	52338166.88	20926271.79	26410328.91	44295220.07	75330194.07	172427915.8
PG(18:1_22:6)	11848612.63	3790892.768	3763178.804	2976821.834	7351704.883	14940184.62	11308055.56	3500456.947	3674834.441	3493161.354	6795556.014	4469370.976	5220817.731	1467986.691	1714562.517	3385101.345	6607402.105	13857959.21
PG(18:1_20:1)	4483802.675	2409132.041	2221598.766	1648804.239	2501987.528	6665550.372	4460031.793	2376173.86	2543942.19	2261249.386	1981464.732	1308329.348	1708090.898	970305.3511	1284077.727	2131170.455	2275537.005	4807922.823
PG(18:1_18:2)	2824339.023	1738116.394	1577789.197	1465824.347	2488961.73	5867776.849	4219632.018	2094745.87	2104210.396	1747946.816	3508449.346	1975243.148	2631542.65	573861.1427	702080.7665	1114285.347	1756601.231	4149642.416
PG(18:1_22:5)	3987303.386	1417531.424	1401564.752	1144358.862	2148487.453	5076575.244	3537861.556	1192960.861	1416428.122	1297748.823	2184250.759	1348988.746	1578536.344	544006.6152	646291.3788	1237103.666	1730198.327	4301705.208
PG(18:1_20:2)	2792083.136	997067.3942	791724.7855	662649.4852	1687636.206	4798697.562	3402050.23	1189524.895	1633157.939	1286242.143	1528052.024	938132.6491	1291468.427	629359.5923	504001.3329	1015998.14	1607734.743	3414236.067
PI(18:0_18:1)	8408761.222	3060691.969	2690423.084	2622466.34	2091310.709	4179413.103	4160068.566	3777475.697	4307638.793	4410297.072	3868945.285	2171619.263	4501862.897	249584.1922	1424659.615	1997397.358	3317289.541	6322296.615
PI(18:0_20:2)	8752519.21	3317829.22	2980266.188	2658321.501	2920939.919	5653959.935	5564039.09	4080574.957	4445337.176	4248702.806	2025439.649	1265887.246	2200908.883	392294.1132	1478120.732	2255147.766	4005941.967	6628585.517
PI(18:0_20:3)	5704434.304	2751278.529	2839942.98	2569617.354	1875613.84	3162102.941	3259714.009	3426341.464	3801521.068	3710109.76	3030446.576	1899922.105	3678552.991	291173.3618	1259630.919	1936401.501	2602031.637	4229105.946
PI(18:1_20:4)	37292145.66	24531354.65	22224168.48	22063227.67	13870091.31	25019397.86	24574383.99	23084117.22	24825750.24	24404622.6	23361325.35	13172631.02	24887443.73	3778974.536	10987719.15	16844164.07	15506897.25	27316983.16
PI(18:1_18:1)	22858442.75	12139920.23	11354418.66	12122341.56	7356100.628	14207257.5	13588129.51	16011627.94	17032712.33	17840552.71	15061252.93	8335572.494	16717899.36	2285502.215	6448884.647	9112369.52	9158126.882	16625920.2
PI(18:1_20:2)	4247168.72	1942646.66	1846186.769	1543937.358	1455856.992	2959704.64	2845667.358	1858407.972	2171317.345	2078219.907	732499.4763	447509.3987	895299.19	387505.7258	891645.4265	1739768.119	1895662.913	3127590.226
Plasmenyl-PE(P-18:1/20:4)	1634078.838	510791.3263	895135.8739	810100.7	845832.495	2488422.45	1579019.308	633999.4489	936704.5453	916694.7431	1195237.007	609383.8107	1006034.479	351876.7841	351148.0293	279172.5076	580942.674	1854995.215
Plasmenyl-PE(P-18:0/20:4)	1062671.808	921307.8906	873066.096	728145.0246	485046.8173	1808076.004	1113468.071	812422.8057	845908.6921	875366.7004	810805.7282	516456.8322	757321.6672	273187.415	261314.6403	464822.4351	391146.1666	1157186.797
Plasmenyl-PE(P-20:0/20:4)	692382.6003	703524.6257	621006.6545	480369.8554	325093.0397	1275256.584	779453.9992	484571.9469	532115.6477	499764.4979	449094.2756	365638.0995	387093.0011	180249.3274	238896.4437	374067.1533	362604.8143	839968.2642
PS(16:0_20:4)	3307964.664	1097634.686	995893.5057	910373.7801	1135488.476	1327679.516	1896418.372	938962.9482	937800.2249	797751.7448	1240132.283	841765.6712	1236389.943	64501.86099	395714.9408	679117.1027	1303534.917	2827468.908
PS(16:0_16:1)	4052276.519	878716.1512	840098.3871	700412.763	1206781.72	2107002.073	2439228.745	1108623.115	1161747.703	1073188.406	901526.5038	456655.6467	1079284.473	57830.19584	405227.0572	377977.1469	1686147.526	3541646.349
PS(16:1_18:0)	12053253.43	3743164.18	3775748.34	3948273.557	4557246.836	7852996.459	8568651.677	5631226.466	5570411.589	5468976.936	5546636.58	3558908.601	5976365.292	905977.4545	2171700.394	3040755.534	5970052.175	10357811.38
PS(16:1_18:1)	3094962.209	1447728.649	1384320.169	1236706.673	1112773.097	1906007.726	2301473.605	1821501.041	1533648.916	1557633.585	1578431.966	829131.5346	1628839.967	85116.09989	567145.8968	303559.8635	1313351.558	2423735.555
PS(18:0_18:1)	34251866.46	19179280.93	18193128.67	18534715.65	11562706.89	23401883.6	25303693.16	21838175.17	24465137.77	23394411.69	23327594.21	14055380.33	26956536.81	3032219.728	10702879.28	16041125.93	15753679.62	29698386.99
PS(18:0_20:4)	44912554.5	19552066.1	18161554.24	17326314.28	16894121.54	28121843.81	31653783.04	17675982.1	18548425.25	17169776.55	21283207.29	12698344.02	23631086.7	3455837.92	9858886.691	14357115.76	22069152.9	40702433.08
PS(18:0_20:3)	10466556.63	5894117.062	5372768.549	5039776.525	4391516.656	5023780.741	8069127.225	6289240.017	6621274.655	4818826.277	5657710.841	3377977.577	6610269.757	1008974.846	3569639.264	5518911.998	6248786.587	9383380.301
PS(18:0_18:2)	7481192.708	2394464.457	2429521.894	1790873.59	3196994.109	5075255.355	5867910.094	3315900.081	3542705.903	3215574.781	3699301.318	2329250.227	3977622.593	436119.3087	1452858.133	1777228.643	3907344.059	6811665.772
PS(18:0_22:5)	7405260.43	3036645.959	2720653.239	2384098.984	2979442.889	3905514.043	5321146.146	2660059.591	2820802.098	3006499.847	4387236.212	2594903.732	4864534.436	283645.2437	1327972.575	2809876.064	3363133.496	6642836.707
PS(18:0_22:4)	5604185.431	1827407.92	1810241.24	1619236.834	1935169.032	3211286.532	4082897.95	1806152.66	2114322.219	1832646.341	2720409.135	1637209.807	3170174.471	441167.412	1150837.283	1765523.202	2743557.246	5010221.979
PS(18:1_18:1)	13596098.19	11825767.85	10945783.35	12221374.85	4915081.281	9030257.298	10442670.57	12552733.39	13737769.48	13420756.04	12793507.03	7060078.819	13705995.54	1398694.628	5029394.305	7778071.151	6270640.736	11651621.02
PS(18:1_20:4)	6563207.045	3862174.249	3984109.286	3742224.688	2444929.004	3980877.34	4717227.737	3353629.887	3589841.065	3287758.617	4111526.06	2251742.699	4072707.303	268905.9796	1293803.354	1967117.921	2674081.965	5553913.882
PS(18:2_18:2)	5454373.508	5081929.16	4770283.619	3712026.181	3935745.161	5548441.481	5936962.239	4879051.779	4831317.923	5682352.078	4841423.256	2198515.727	4601396.053	1888180.349	2798930.51	3643064.022	4591670.985	6777254.577
PS(18:2_20:3)	3812382.41	1617242.833	1602108.481	2268118.907	1670693.717	3851425.323	2904837.334	1934865.25	2234582.182	2873165.832	2296747.446	900219.7772	1538982.124	847414.6741	1331667.63	1778719.608	1889523.87	3455007.523
PS(20:2_20:2)	1270468.443	614741.0193	643328.3646	608510.6725	484047.6578	1614321.01	1003462.579	909552.1118	1011689.598	1032165.127	565768.3156	322898.1664	481871.4133	462987.3127	665465.5746	860884.7973	589222.4115	1255538.379
PS(20:4_20:4)	4306534.43	1996198.276	1624163.28	1653890.02	2261871.091	5822874.947	3822949.642	2585489.698	2941276.365	2498941.384	1974234.505	1578764.112	1946922.609	497220.6907	671039.5725	1111163.056	2140352.55	4395896.954
SM(d18:1/16:1)	1266057.884	879600.5876	613848.6067	787282.2625	831700.4298	1748533.492	771031.2879	964380.2723	868319.9817	1083043.164	975695.5078	845292.8827	988855.0037	376173.0938	522734.7721	710721.2391	684991.2082	1142843.808
SM(d22:1/18:1)	4930407.209	2795273.536	3021635.834	2463733.588	2295443.036	6985986.882	4439584.057	2635555.014	3113249.746	2799095.583	2961409.206	2058337.436	2995490.956	1027000.11	1605029.602	1874446.009	2108389.073	5542229.279
SM(41:1)	4430861.268	3067825.572	3189214.316	2712026.744	2049600.135	6386616.25	3921465.202	3037641.035	3713981.069	3239681.448	2864309.597	2169393.062	2844442.567	893583.748	1222140.28	1727774.277	1812696.781	4274667.212
SM(42:1)	1809257.665	1302684.964	1271973.183	956803.7696	572540.2886	2473912.644	1613422.653	1819471.033	2166343.181	1918748.914	1907931.568	1015722.076	1854598.85	346859.742	531633.0417	740446.1819	1000544.935	2077871.85
MS_METABOLITE_DATA_END
#METABOLITES
METABOLITES_START
metabolite_name	m/z	RT
Cer-NS(d18:2/24:1)	690.6046835	17.29565079
Cer-NS(d18:1/22:0)	666.6046566	18.49598413
Cer-NS(d18:2/16:0)	580.4944615	12.35470714
Cer-NS(d18:1/16:0)	536.5048203	13.9442127
Cer-NS(d18:1/24:1)	646.6145888	18.2419127
DMPE(14:0_16:0)	690.5078518	12.31905159
DMPE(16:0_18:1)	744.5545706	14.17296746
DMPE(16:0_16:1)	716.5234292	12.66117857
DMPE(16:0_16:0)	718.5395392	14.10195794
DMPE(16:1_18:1)	742.5392068	12.82561746
DMPE(18:1_20:1)	798.6026608	17.37211905
LPC(16:0)	540.3307935	2.818269048
LPC(18:0)	568.3621036	4.601188889
LPC(18:1)	566.3464662	2.770634127
LPE(16:0)	452.2782509	2.977934921
LPE(18:0)	480.3094989	4.857834127
LPE(18:1)	478.2939229	3.199497619
PC(14:0_16:0)	750.5295223	12.31822143
PC(14:0_16:1)	748.5137552	10.84329365
PC(14:0_14:0)	722.4981565	10.50391587
PC(15:0_16:1)	762.5299738	11.57755079
PC(16:0_18:1)	804.5766401	14.17586825
PC(16:0_16:1)	776.5452205	12.66536587
PC(16:0_16:0)	778.5607976	14.10280714
PC(16:0_20:4)	826.5608244	12.64280397
PC(16:0_20:1)	832.6082008	15.74518889
PC(16:0_18:0)	806.5922168	15.90675635
PC(16:0_22:6)	850.5602766	12.23456825
PC(16:0_20:5)	824.5452727	11.56927143
PC(16:0_22:4)	854.5923516	13.83932143
PC(16:0_22:5)	852.5766395	13.36575635
PC(16:0_20:3)	828.5767014	13.91188095
PC(16:1_18:1)	802.5609102	12.79533254
PC(16:1_16:1)	774.5294961	11.1908254
PC(16:1_20:4)	824.545113	11.16380714
PC(16:1_18:2)	800.54511	11.40054206
PC(16:1_17:1)	788.5452223	11.92964127
PC(17:0_18:1)	818.594036	14.66737937
PC(18:0_18:1)	832.608168	15.95489206
PC(18:0_18:2)	830.5926726	14.69608254
PC(18:0_20:3)	856.6079847	15.12875635
PC(18:0_22:6)	878.591817	14.05733889
PC(18:0_22:4)	882.6238098	15.60102619
PC(18:1_18:1)	830.5924607	14.26032857
PC(18:1_18:2)	828.5764273	12.94946032
PC(18:1_20:4)	852.5762543	12.72309286
PC(18:1_20:1)	858.623653	15.82674762
PC(18:1_22:6)	876.5759348	12.31863571
PC(18:1_20:5)	850.5606953	11.64768095
PC(18:1_20:2)	856.6074737	14.85305873
PC(18:1_22:1)	886.6547792	17.18174603
PC(18:2_20:1)	856.6068605	14.55220397
PC(20:1_20:4)	880.6072259	14.34258175
PE(16:0_18:1)	716.5241033	14.5539746
PE(16:0_20:4)	738.5082318	13.00757698
PE(16:0_20:3)	740.5241582	13.69060714
PE(16:0_20:5)	736.4928005	11.92732937
PE(16:0_18:0)	718.539627	16.22457222
PE(16:1_18:1)	714.5081571	13.19237937
PE(16:1_18:0)	716.5242296	14.87586587
PE(16:1_20:4)	736.4910642	11.36860159
PE(16:1_22:6)	760.4923085	10.97861587
PE(16:1_18:2)	712.4926087	11.60179286
PE(17:0_20:4)	752.5242091	13.91550873
PE(18:0_20:4)	766.539836	14.81434286
PE(18:0_18:2)	742.540056	15.0441
PE(18:0_22:6)	790.5395676	14.41371667
PE(18:0_20:3)	768.5555039	15.4588246
PE(18:0_20:5)	764.5238978	13.75696667
PE(18:0_22:5)	792.5544562	14.88951746
PE(18:0_22:4)	794.5709971	15.91144841
PE(18:0_20:2)	770.571181	16.75781746
PE(18:0_20:1)	772.5866275	17.44088889
PE(18:0_22:2)	798.602652	17.64526984
PE(18:1_18:1)	742.5398958	14.62476984
PE(18:1_20:4)	764.5228426	13.0818627
PE(18:1_18:2)	740.5240386	13.31533016
PE(18:1_22:6)	788.5235589	12.67509286
PE(18:1_20:1)	770.5711014	16.1285
PE(18:1_20:3)	766.5396263	13.75957302
PE(18:1_20:5)	762.507737	12.00636905
PE(18:1_22:5)	790.5380758	13.75161429
PE(18:1_20:2)	768.555525	15.22238175
PE(18:1_22:2)	796.5863792	16.4271873
PE(19:0_20:4)	780.5551481	15.69423651
PE(20:0_20:4)	794.5712446	16.49827222
PE(20:1_20:4)	792.5544476	14.69518254
PE(20:1_22:6)	816.5551598	14.3012
PE(20:1_20:3)	794.5706234	15.39552619
PE(20:4_22:1)	820.5864067	16.25562857
PE(20:4_22:0)	822.6024526	17.80320635
PE(20:4_24:1)	848.6171477	17.52221429
PG(16:0_18:1)	747.5184925	13.33495952
PG(16:1_18:1)	745.5027835	10.94001032
PG(17:1_18:1)	759.5184356	11.55840556
PG(18:0_18:1)	775.5507133	15.04168095
PG(18:1_18:1)	773.5340835	12.35469206
PG(18:1_22:6)	819.5186286	10.63055079
PG(18:1_20:1)	801.566449	13.83398175
PG(18:1_18:2)	771.5185726	11.15277063
PG(18:1_22:5)	821.5342707	11.61391746
PG(18:1_20:2)	799.5497119	12.64395
PI(18:0_18:1)	863.5654709	14.79152143
PI(18:0_20:2)	889.581943	15.39158651
PI(18:0_20:3)	887.5657202	13.98364444
PI(18:1_20:4)	883.5347634	11.70695635
PI(18:1_18:1)	861.5502163	13.14858016
PI(18:1_20:2)	887.5659823	13.78894048
Plasmenyl-PE(P-18:1/20:4)	748.5290196	13.92946587
Plasmenyl-PE(P-18:0/20:4)	750.5450527	15.64018571
Plasmenyl-PE(P-20:0/20:4)	778.5755989	17.16766667
PS(16:0_20:4)	782.4978914	11.74949444
PS(16:0_16:1)	732.4825367	11.75171667
PS(16:1_18:0)	760.5140607	13.55354603
PS(16:1_18:1)	758.4980763	11.87269286
PS(18:0_18:1)	788.5455442	14.98406508
PS(18:0_20:4)	810.5295965	13.53239048
PS(18:0_20:3)	812.5443903	14.16382222
PS(18:0_18:2)	786.5296238	13.72472619
PS(18:0_22:5)	836.5441567	13.60864127
PS(18:0_22:4)	838.5606963	14.64769365
PS(18:1_18:1)	786.5295996	13.31951825
PS(18:1_20:4)	808.5138239	11.84858175
PS(18:2_18:2)	782.4954277	13.20879683
PS(18:2_20:3)	808.5116666	13.69781905
PS(20:2_20:2)	838.5584877	16.75499206
PS(20:4_20:4)	830.4958035	12.00212143
SM(d18:1/16:1)	745.5493991	10.91634762
SM(d22:1/18:1)	829.6447556	16.29581746
SM(41:1)	845.676107	18.09850794
SM(42:1)	859.6904121	17.63339683
METABOLITES_END
#END