#METABOLOMICS WORKBENCH YjChern_20250828_201310 DATATRACK_ID:6345 STUDY_ID:ST004164 ANALYSIS_ID:AN006912 PROJECT_ID:PR002624
VERSION             	1
CREATED_ON             	September 2, 2025, 8:11 pm
#PROJECT
PR:PROJECT_TITLE                 	Equilibrative Nucleoside Transporter 2 Modulates Inosine Catabolism to Influence
PR:PROJECT_TITLE                 	Astrocyte Metabolism and Reactivity
PR:PROJECT_SUMMARY               	Astrocytes are central regulators of brain function and exhibit diverse reactive
PR:PROJECT_SUMMARY               	states in response to injury and inflammation. Although the mechanisms
PR:PROJECT_SUMMARY               	underlying these states remain debated, cellular energy metabolism is thought to
PR:PROJECT_SUMMARY               	play a crucial role. This project aimed to define the role of equilibrative
PR:PROJECT_SUMMARY               	nucleoside transporter 2 (Ent2) in regulating astrocytic responses to
PR:PROJECT_SUMMARY               	inflammatory stimulation, with a particular focus on its function in inosine
PR:PROJECT_SUMMARY               	metabolism and energy balance. Using mouse primary astrocytes exposed to
PR:PROJECT_SUMMARY               	cytokine-induced activation, together with targeted metabolomic profiling and
PR:PROJECT_SUMMARY               	pharmacological manipulation, we examined how Ent2 activity influences purine
PR:PROJECT_SUMMARY               	metabolism and reactivity. Our findings show that inosine is an essential energy
PR:PROJECT_SUMMARY               	substrate during inflammation and that Ent2 is required to regulate inosine
PR:PROJECT_SUMMARY               	efflux and maintain ATP production. Loss of Ent2, or pharmacological inhibition
PR:PROJECT_SUMMARY               	of inosine catabolism, disrupted energy homeostasis, impaired mitochondrial
PR:PROJECT_SUMMARY               	function, and enhanced astrocytic reactivity. These results establish an
PR:PROJECT_SUMMARY               	Ent2-inosine-PNP axis as a critical pathway linking purine metabolism to
PR:PROJECT_SUMMARY               	astrocytic reactivity.
PR:INSTITUTE                     	Institute of Biomedical Sciences, Academia Sinica
PR:LAST_NAME                     	Chern
PR:FIRST_NAME                    	Yijuang
PR:ADDRESS                       	128 Section 2, Academia Road, Nankang, Taipei, 115201, Taiwan
PR:EMAIL                         	bmychern@ibms.sinica.edu.tw
PR:PHONE                         	+886-2-2652-3913
#STUDY
ST:STUDY_TITLE                   	Equilibrative Nucleoside Transporter 2 Modulates Inosine Catabolism to Influence
ST:STUDY_TITLE                   	Astrocyte Metabolism and Reactivity
ST:STUDY_SUMMARY                 	In this study, we demonstrate that inosine functions as an essential energy
ST:STUDY_SUMMARY                 	substrate for astrocytes under inflammatory conditions. Targeted metabolomic
ST:STUDY_SUMMARY                 	datasets were generated from mouse primary astrocytes after 24 hours of cytokine
ST:STUDY_SUMMARY                 	cocktail treatment. Metabolomic profiling, combined with pharmacological
ST:STUDY_SUMMARY                 	approaches, revealed that Ent2 regulates inosine efflux and is critical for
ST:STUDY_SUMMARY                 	maintaining astrocytic energy balance. Pharmacological inhibition of inosine
ST:STUDY_SUMMARY                 	utilization in energy-producing pathways with Forodesine led to disrupted energy
ST:STUDY_SUMMARY                 	homeostasis and increased astrocytic reactivity. The datasets include both raw
ST:STUDY_SUMMARY                 	and processed metabolomic files. Metabolomic analyses were performed at the
ST:STUDY_SUMMARY                 	Metabolomics Core Facility, Agricultural Biotechnology Research Center, Academia
ST:STUDY_SUMMARY                 	Sinica, Taipei, Taiwan.
ST:INSTITUTE                     	Institute of Biomedical Sciences, Academia Sinica
ST:LAST_NAME                     	Chern
ST:FIRST_NAME                    	Yijuang
ST:ADDRESS                       	128 Section 2, Academia Road, Nankang, Taipei, 115201, Taiwan
ST:EMAIL                         	bmychern@ibms.sinica.edu.tw
ST:PHONE                         	+886-2-26523913
#SUBJECT
SU:SUBJECT_TYPE                  	Cultured cells
SU:SUBJECT_SPECIES               	Mus musculus
SU:TAXONOMY_ID                   	10090
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data
SUBJECT_SAMPLE_FACTORS           	WT_1_Ctrl	WT_1_Ctrl	Sample source:mouse primary astrocytes | Genotype:Wild-type | treatment:Vehicle	Batch=B1; MS_ion Mode=negative; RAW_FILE_NAME=WT_1_Ctrl.mzML
SUBJECT_SAMPLE_FACTORS           	WT_2_Ctrl	WT_2_Ctrl	Sample source:mouse primary astrocytes | Genotype:Wild-type | treatment:Vehicle	Batch=B1; MS_ion Mode=negative; RAW_FILE_NAME=WT_2_Ctrl.mzML
SUBJECT_SAMPLE_FACTORS           	WT_3_Ctrl	WT_3_Ctrl	Sample source:mouse primary astrocytes | Genotype:Wild-type | treatment:Vehicle	Batch=B1; MS_ion Mode=negative; RAW_FILE_NAME=WT_3_Ctrl.mzML
SUBJECT_SAMPLE_FACTORS           	WT_4_Ctrl	WT_4_Ctrl	Sample source:mouse primary astrocytes | Genotype:Wild-type | treatment:Vehicle	Batch=B2; MS_ion Mode=negative; RAW_FILE_NAME=WT_4_Ctrl.mzML
SUBJECT_SAMPLE_FACTORS           	WT_5_Ctrl	WT_5_Ctrl	Sample source:mouse primary astrocytes | Genotype:Wild-type | treatment:Vehicle	Batch=B2; MS_ion Mode=negative; RAW_FILE_NAME=WT_5_Ctrl.mzML
SUBJECT_SAMPLE_FACTORS           	WT_6_Ctrl	WT_6_Ctrl	Sample source:mouse primary astrocytes | Genotype:Wild-type | treatment:Vehicle	Batch=B2; MS_ion Mode=negative; RAW_FILE_NAME=WT_6_Ctrl.mzML
SUBJECT_SAMPLE_FACTORS           	WT_1_Cytokine	WT_1_Cytokine	Sample source:mouse primary astrocytes | Genotype:Wild-type | treatment:TNF𝝰/IL-1𝝰	Batch=B1; MS_ion Mode=negative; RAW_FILE_NAME=WT_1_Cytokine.mzML
SUBJECT_SAMPLE_FACTORS           	WT_2_Cytokine	WT_2_Cytokine	Sample source:mouse primary astrocytes | Genotype:Wild-type | treatment:TNF𝝰/IL-1𝝰	Batch=B1; MS_ion Mode=negative; RAW_FILE_NAME=WT_2_Cytokine.mzML
SUBJECT_SAMPLE_FACTORS           	WT_3_Cytokine	WT_3_Cytokine	Sample source:mouse primary astrocytes | Genotype:Wild-type | treatment:TNF𝝰/IL-1𝝰	Batch=B1; MS_ion Mode=negative; RAW_FILE_NAME=WT_3_Cytokine.mzML
SUBJECT_SAMPLE_FACTORS           	WT_4_Cytokine	WT_4_Cytokine	Sample source:mouse primary astrocytes | Genotype:Wild-type | treatment:TNF𝝰/IL-1𝝰	Batch=B2; MS_ion Mode=negative; RAW_FILE_NAME=WT_4_Cytokine.mzML
SUBJECT_SAMPLE_FACTORS           	WT_5_Cytokine	WT_5_Cytokine	Sample source:mouse primary astrocytes | Genotype:Wild-type | treatment:TNF𝝰/IL-1𝝰	Batch=B2; MS_ion Mode=negative; RAW_FILE_NAME=WT_5_Cytokine.mzML
SUBJECT_SAMPLE_FACTORS           	WT_6_Cytokine	WT_6_Cytokine	Sample source:mouse primary astrocytes | Genotype:Wild-type | treatment:TNF𝝰/IL-1𝝰	Batch=B2; MS_ion Mode=negative; RAW_FILE_NAME=WT_6_Cytokine.mzML
SUBJECT_SAMPLE_FACTORS           	WT_7_Cytokine	WT_7_Cytokine	Sample source:mouse primary astrocytes | Genotype:Wild-type | treatment:TNF𝝰/IL-1𝝰	Batch=B2; MS_ion Mode=negative; RAW_FILE_NAME=WT_7_Cytokine.mzML
SUBJECT_SAMPLE_FACTORS           	WT_1_CytokinePlusForo	WT_1_CytokinePlusForo	Sample source:mouse primary astrocytes | Genotype:Wild-type | treatment:TNF𝝰/IL-1𝝰/Forodesine	Batch=B1; MS_ion Mode=negative; RAW_FILE_NAME=WT_1_CytokinePlusForo.mzML
SUBJECT_SAMPLE_FACTORS           	WT_2_CytokinePlusForo	WT_2_CytokinePlusForo	Sample source:mouse primary astrocytes | Genotype:Wild-type | treatment:TNF𝝰/IL-2𝝰/Forodesine	Batch=B1; MS_ion Mode=negative; RAW_FILE_NAME=WT_2_CytokinePlusForo.mzML
SUBJECT_SAMPLE_FACTORS           	WT_3_CytokinePlusForo	WT_3_CytokinePlusForo	Sample source:mouse primary astrocytes | Genotype:Wild-type | treatment:TNF𝝰/IL-3𝝰/Forodesine	Batch=B1; MS_ion Mode=negative; RAW_FILE_NAME=WT_3_CytokinePlusForo.mzML
SUBJECT_SAMPLE_FACTORS           	WT_4_CytokinePlusForo	WT_4_CytokinePlusForo	Sample source:mouse primary astrocytes | Genotype:Wild-type | treatment:TNF𝝰/IL-4𝝰/Forodesine	Batch=B1; MS_ion Mode=negative; RAW_FILE_NAME=WT_4_CytokinePlusForo.mzML
SUBJECT_SAMPLE_FACTORS           	WT_5_CytokinePlusForo	WT_5_CytokinePlusForo	Sample source:mouse primary astrocytes | Genotype:Wild-type | treatment:TNF𝝰/IL-5𝝰/Forodesine	Batch=B2; MS_ion Mode=negative; RAW_FILE_NAME=WT_5_CytokinePlusForo.mzML
SUBJECT_SAMPLE_FACTORS           	WT_6_CytokinePlusForo	WT_6_CytokinePlusForo	Sample source:mouse primary astrocytes | Genotype:Wild-type | treatment:TNF𝝰/IL-6𝝰/Forodesine	Batch=B2; MS_ion Mode=negative; RAW_FILE_NAME=WT_6_CytokinePlusForo.mzML
SUBJECT_SAMPLE_FACTORS           	WT_7_CytokinePlusForo	WT_7_CytokinePlusForo	Sample source:mouse primary astrocytes | Genotype:Wild-type | treatment:TNF𝝰/IL-7𝝰/Forodesine	Batch=B2; MS_ion Mode=negative; RAW_FILE_NAME=WT_7_CytokinePlusForo.mzML
SUBJECT_SAMPLE_FACTORS           	KO_1_Ctrl	KO_1_Ctrl	Sample source:mouse primary astrocytes | Genotype:Ent2 knockout | treatment:Vehicle	Batch=B1; MS_ion Mode=negative; RAW_FILE_NAME=KO_1_Ctrl.mzML
SUBJECT_SAMPLE_FACTORS           	KO_2_Ctrl	KO_2_Ctrl	Sample source:mouse primary astrocytes | Genotype:Ent3 knockout | treatment:Vehicle	Batch=B1; MS_ion Mode=negative; RAW_FILE_NAME=KO_2_Ctrl.mzML
SUBJECT_SAMPLE_FACTORS           	KO_3_Ctrl	KO_3_Ctrl	Sample source:mouse primary astrocytes | Genotype:Ent4 knockout | treatment:Vehicle	Batch=B1; MS_ion Mode=negative; RAW_FILE_NAME=KO_3_Ctrl.mzML
SUBJECT_SAMPLE_FACTORS           	KO_4_Ctrl	KO_4_Ctrl	Sample source:mouse primary astrocytes | Genotype:Ent5 knockout | treatment:Vehicle	Batch=B2; MS_ion Mode=negative; RAW_FILE_NAME=KO_4_Ctrl.mzML
SUBJECT_SAMPLE_FACTORS           	KO_5_Ctrl	KO_5_Ctrl	Sample source:mouse primary astrocytes | Genotype:Ent6 knockout | treatment:Vehicle	Batch=B2; MS_ion Mode=negative; RAW_FILE_NAME=KO_5_Ctrl.mzML
SUBJECT_SAMPLE_FACTORS           	KO_6_Ctrl	KO_6_Ctrl	Sample source:mouse primary astrocytes | Genotype:Ent7 knockout | treatment:Vehicle	Batch=B2; MS_ion Mode=negative; RAW_FILE_NAME=KO_6_Ctrl.mzML
SUBJECT_SAMPLE_FACTORS           	KO_1_Cytokine	KO_1_Cytokine	Sample source:mouse primary astrocytes | Genotype:Ent8 knockout | treatment:TNF𝝰/IL-1𝝰	Batch=B1; MS_ion Mode=negative; RAW_FILE_NAME=KO_1_Cytokine.mzML
SUBJECT_SAMPLE_FACTORS           	KO_2_Cytokine	KO_2_Cytokine	Sample source:mouse primary astrocytes | Genotype:Ent9 knockout | treatment:TNF𝝰/IL-1𝝰	Batch=B1; MS_ion Mode=negative; RAW_FILE_NAME=KO_2_Cytokine.mzML
SUBJECT_SAMPLE_FACTORS           	KO_3_Cytokine	KO_3_Cytokine	Sample source:mouse primary astrocytes | Genotype:Ent10 knockout | treatment:TNF𝝰/IL-1𝝰	Batch=B1; MS_ion Mode=negative; RAW_FILE_NAME=KO_3_Cytokine.mzML
SUBJECT_SAMPLE_FACTORS           	KO_4_Cytokine	KO_4_Cytokine	Sample source:mouse primary astrocytes | Genotype:Ent11 knockout | treatment:TNF𝝰/IL-1𝝰	Batch=B2; MS_ion Mode=negative; RAW_FILE_NAME=KO_4_Cytokine.mzML
SUBJECT_SAMPLE_FACTORS           	KO_5_Cytokine	KO_5_Cytokine	Sample source:mouse primary astrocytes | Genotype:Ent12 knockout | treatment:TNF𝝰/IL-1𝝰	Batch=B2; MS_ion Mode=negative; RAW_FILE_NAME=KO_5_Cytokine.mzML
SUBJECT_SAMPLE_FACTORS           	KO_6_Cytokine	KO_6_Cytokine	Sample source:mouse primary astrocytes | Genotype:Ent13 knockout | treatment:TNF𝝰/IL-1𝝰	Batch=B2; MS_ion Mode=negative; RAW_FILE_NAME=KO_6_Cytokine.mzML
SUBJECT_SAMPLE_FACTORS           	KO_7_Cytokine	KO_7_Cytokine	Sample source:mouse primary astrocytes | Genotype:Ent14 knockout | treatment:TNF𝝰/IL-1𝝰	Batch=B2; MS_ion Mode=negative; RAW_FILE_NAME=KO_7_Cytokine.mzML
SUBJECT_SAMPLE_FACTORS           	KO_1_CytokinePlusForo	KO_1_CytokinePlusForo	Sample source:mouse primary astrocytes | Genotype:Ent15 knockout | treatment:TNF𝝰/IL-1𝝰/Forodesine	Batch=B1; MS_ion Mode=negative; RAW_FILE_NAME=KO_1_CytokinePlusForo.mzML
SUBJECT_SAMPLE_FACTORS           	KO_2_CytokinePlusForo	KO_2_CytokinePlusForo	Sample source:mouse primary astrocytes | Genotype:Ent16 knockout | treatment:TNF𝝰/IL-2𝝰/Forodesine	Batch=B1; MS_ion Mode=negative; RAW_FILE_NAME=KO_2_CytokinePlusForo.mzML
SUBJECT_SAMPLE_FACTORS           	KO_3_CytokinePlusForo	KO_3_CytokinePlusForo	Sample source:mouse primary astrocytes | Genotype:Ent17 knockout | treatment:TNF𝝰/IL-3𝝰/Forodesine	Batch=B1; MS_ion Mode=negative; RAW_FILE_NAME=KO_3_CytokinePlusForo.mzML
SUBJECT_SAMPLE_FACTORS           	KO_4_CytokinePlusForo	KO_4_CytokinePlusForo	Sample source:mouse primary astrocytes | Genotype:Ent18 knockout | treatment:TNF𝝰/IL-4𝝰/Forodesine	Batch=B1; MS_ion Mode=negative; RAW_FILE_NAME=KO_4_CytokinePlusForo.mzML
SUBJECT_SAMPLE_FACTORS           	KO_5_CytokinePlusForo	KO_5_CytokinePlusForo	Sample source:mouse primary astrocytes | Genotype:Ent19 knockout | treatment:TNF𝝰/IL-5𝝰/Forodesine	Batch=B2; MS_ion Mode=negative; RAW_FILE_NAME=KO_5_CytokinePlusForo.mzML
SUBJECT_SAMPLE_FACTORS           	KO_6_CytokinePlusForo	KO_6_CytokinePlusForo	Sample source:mouse primary astrocytes | Genotype:Ent20 knockout | treatment:TNF𝝰/IL-6𝝰/Forodesine	Batch=B2; MS_ion Mode=negative; RAW_FILE_NAME=KO_6_CytokinePlusForo.mzML
SUBJECT_SAMPLE_FACTORS           	KO_7_CytokinePlusForo	KO_7_CytokinePlusForo	Sample source:mouse primary astrocytes | Genotype:Ent21 knockout | treatment:TNF𝝰/IL-7𝝰/Forodesine	Batch=B2; MS_ion Mode=negative; RAW_FILE_NAME=KO_7_CytokinePlusForo.mzML
SUBJECT_SAMPLE_FACTORS           	p_WT_1_Ctrl	p_WT_1_Ctrl	Sample source:mouse primary astrocytes | Genotype:Wild-type | treatment:Vehicle	Batch=B1; MS_ion Mode=positive; RAW_FILE_NAME=p_WT_1_Ctrl.mzML
SUBJECT_SAMPLE_FACTORS           	p_WT_2_Ctrl	p_WT_2_Ctrl	Sample source:mouse primary astrocytes | Genotype:Wild-type | treatment:Vehicle	Batch=B1; MS_ion Mode=positive; RAW_FILE_NAME=p_WT_2_Ctrl.mzML
SUBJECT_SAMPLE_FACTORS           	p_WT_3_Ctrl	p_WT_3_Ctrl	Sample source:mouse primary astrocytes | Genotype:Wild-type | treatment:Vehicle	Batch=B1; MS_ion Mode=positive; RAW_FILE_NAME=p_WT_3_Ctrl.mzML
SUBJECT_SAMPLE_FACTORS           	p_WT_4_Ctrl	p_WT_4_Ctrl	Sample source:mouse primary astrocytes | Genotype:Wild-type | treatment:Vehicle	Batch=B2; MS_ion Mode=positive; RAW_FILE_NAME=p_WT_4_Ctrl.mzML
SUBJECT_SAMPLE_FACTORS           	p_WT_5_Ctrl	p_WT_5_Ctrl	Sample source:mouse primary astrocytes | Genotype:Wild-type | treatment:Vehicle	Batch=B2; MS_ion Mode=positive; RAW_FILE_NAME=p_WT_5_Ctrl.mzML
SUBJECT_SAMPLE_FACTORS           	p_WT_6_Ctrl	p_WT_6_Ctrl	Sample source:mouse primary astrocytes | Genotype:Wild-type | treatment:Vehicle	Batch=B2; MS_ion Mode=positive; RAW_FILE_NAME=p_WT_6_Ctrl.mzML
SUBJECT_SAMPLE_FACTORS           	p_WT_1_Cytokine	p_WT_1_Cytokine	Sample source:mouse primary astrocytes | Genotype:Wild-type | treatment:TNF𝝰/IL-1𝝰	Batch=B1; MS_ion Mode=positive; RAW_FILE_NAME=p_WT_1_Cytokine.mzML
SUBJECT_SAMPLE_FACTORS           	p_WT_2_Cytokine	p_WT_2_Cytokine	Sample source:mouse primary astrocytes | Genotype:Wild-type | treatment:TNF𝝰/IL-1𝝰	Batch=B1; MS_ion Mode=positive; RAW_FILE_NAME=p_WT_2_Cytokine.mzML
SUBJECT_SAMPLE_FACTORS           	p_WT_3_Cytokine	p_WT_3_Cytokine	Sample source:mouse primary astrocytes | Genotype:Wild-type | treatment:TNF𝝰/IL-1𝝰	Batch=B1; MS_ion Mode=positive; RAW_FILE_NAME=p_WT_3_Cytokine.mzML
SUBJECT_SAMPLE_FACTORS           	p_WT_4_Cytokine	p_WT_4_Cytokine	Sample source:mouse primary astrocytes | Genotype:Wild-type | treatment:TNF𝝰/IL-1𝝰	Batch=B2; MS_ion Mode=positive; RAW_FILE_NAME=p_WT_4_Cytokine.mzML
SUBJECT_SAMPLE_FACTORS           	p_WT_5_Cytokine	p_WT_5_Cytokine	Sample source:mouse primary astrocytes | Genotype:Wild-type | treatment:TNF𝝰/IL-1𝝰	Batch=B2; MS_ion Mode=positive; RAW_FILE_NAME=p_WT_5_Cytokine.mzML
SUBJECT_SAMPLE_FACTORS           	p_WT_6_Cytokine	p_WT_6_Cytokine	Sample source:mouse primary astrocytes | Genotype:Wild-type | treatment:TNF𝝰/IL-1𝝰	Batch=B2; MS_ion Mode=positive; RAW_FILE_NAME=p_WT_6_Cytokine.mzML
SUBJECT_SAMPLE_FACTORS           	p_WT_7_Cytokine	p_WT_7_Cytokine	Sample source:mouse primary astrocytes | Genotype:Wild-type | treatment:TNF𝝰/IL-1𝝰	Batch=B2; MS_ion Mode=positive; RAW_FILE_NAME=p_WT_7_Cytokine.mzML
SUBJECT_SAMPLE_FACTORS           	p_WT_1_CytokinePlusForo	p_WT_1_CytokinePlusForo	Sample source:mouse primary astrocytes | Genotype:Wild-type | treatment:TNF𝝰/IL-1𝝰/Forodesine	Batch=B1; MS_ion Mode=positive; RAW_FILE_NAME=p_WT_1_CytokinePlusForo.mzML
SUBJECT_SAMPLE_FACTORS           	p_WT_2_CytokinePlusForo	p_WT_2_CytokinePlusForo	Sample source:mouse primary astrocytes | Genotype:Wild-type | treatment:TNF𝝰/IL-2𝝰/Forodesine	Batch=B1; MS_ion Mode=positive; RAW_FILE_NAME=p_WT_2_CytokinePlusForo.mzML
SUBJECT_SAMPLE_FACTORS           	p_WT_3_CytokinePlusForo	p_WT_3_CytokinePlusForo	Sample source:mouse primary astrocytes | Genotype:Wild-type | treatment:TNF𝝰/IL-3𝝰/Forodesine	Batch=B1; MS_ion Mode=positive; RAW_FILE_NAME=p_WT_3_CytokinePlusForo.mzML
SUBJECT_SAMPLE_FACTORS           	p_WT_4_CytokinePlusForo	p_WT_4_CytokinePlusForo	Sample source:mouse primary astrocytes | Genotype:Wild-type | treatment:TNF𝝰/IL-4𝝰/Forodesine	Batch=B1; MS_ion Mode=positive; RAW_FILE_NAME=p_WT_4_CytokinePlusForo.mzML
SUBJECT_SAMPLE_FACTORS           	p_WT_5_CytokinePlusForo	p_WT_5_CytokinePlusForo	Sample source:mouse primary astrocytes | Genotype:Wild-type | treatment:TNF𝝰/IL-5𝝰/Forodesine	Batch=B2; MS_ion Mode=positive; RAW_FILE_NAME=p_WT_5_CytokinePlusForo.mzML
SUBJECT_SAMPLE_FACTORS           	p_WT_6_CytokinePlusForo	p_WT_6_CytokinePlusForo	Sample source:mouse primary astrocytes | Genotype:Wild-type | treatment:TNF𝝰/IL-6𝝰/Forodesine	Batch=B2; MS_ion Mode=positive; RAW_FILE_NAME=p_WT_6_CytokinePlusForo.mzML
SUBJECT_SAMPLE_FACTORS           	p_WT_7_CytokinePlusForo	p_WT_7_CytokinePlusForo	Sample source:mouse primary astrocytes | Genotype:Wild-type | treatment:TNF𝝰/IL-7𝝰/Forodesine	Batch=B2; MS_ion Mode=positive; RAW_FILE_NAME=p_WT_7_CytokinePlusForo.mzML
SUBJECT_SAMPLE_FACTORS           	p_KO_1_Ctrl	p_KO_1_Ctrl	Sample source:mouse primary astrocytes | Genotype:Ent2 knockout | treatment:Vehicle	Batch=B1; MS_ion Mode=positive; RAW_FILE_NAME=p_KO_1_Ctrl.mzML
SUBJECT_SAMPLE_FACTORS           	p_KO_2_Ctrl	p_KO_2_Ctrl	Sample source:mouse primary astrocytes | Genotype:Ent3 knockout | treatment:Vehicle	Batch=B1; MS_ion Mode=positive; RAW_FILE_NAME=p_KO_2_Ctrl.mzML
SUBJECT_SAMPLE_FACTORS           	p_KO_3_Ctrl	p_KO_3_Ctrl	Sample source:mouse primary astrocytes | Genotype:Ent4 knockout | treatment:Vehicle	Batch=B1; MS_ion Mode=positive; RAW_FILE_NAME=p_KO_3_Ctrl.mzML
SUBJECT_SAMPLE_FACTORS           	p_KO_4_Ctrl	p_KO_4_Ctrl	Sample source:mouse primary astrocytes | Genotype:Ent5 knockout | treatment:Vehicle	Batch=B2; MS_ion Mode=positive; RAW_FILE_NAME=p_KO_4_Ctrl.mzML
SUBJECT_SAMPLE_FACTORS           	p_KO_5_Ctrl	p_KO_5_Ctrl	Sample source:mouse primary astrocytes | Genotype:Ent6 knockout | treatment:Vehicle	Batch=B2; MS_ion Mode=positive; RAW_FILE_NAME=p_KO_5_Ctrl.mzML
SUBJECT_SAMPLE_FACTORS           	p_KO_6_Ctrl	p_KO_6_Ctrl	Sample source:mouse primary astrocytes | Genotype:Ent7 knockout | treatment:Vehicle	Batch=B2; MS_ion Mode=positive; RAW_FILE_NAME=p_KO_6_Ctrl.mzML
SUBJECT_SAMPLE_FACTORS           	p_KO_1_Cytokine	p_KO_1_Cytokine	Sample source:mouse primary astrocytes | Genotype:Ent8 knockout | treatment:TNF𝝰/IL-1𝝰	Batch=B1; MS_ion Mode=positive; RAW_FILE_NAME=p_KO_1_Cytokine.mzML
SUBJECT_SAMPLE_FACTORS           	p_KO_2_Cytokine	p_KO_2_Cytokine	Sample source:mouse primary astrocytes | Genotype:Ent9 knockout | treatment:TNF𝝰/IL-1𝝰	Batch=B1; MS_ion Mode=positive; RAW_FILE_NAME=p_KO_2_Cytokine.mzML
SUBJECT_SAMPLE_FACTORS           	p_KO_3_Cytokine	p_KO_3_Cytokine	Sample source:mouse primary astrocytes | Genotype:Ent10 knockout | treatment:TNF𝝰/IL-1𝝰	Batch=B1; MS_ion Mode=positive; RAW_FILE_NAME=p_KO_3_Cytokine.mzML
SUBJECT_SAMPLE_FACTORS           	p_KO_4_Cytokine	p_KO_4_Cytokine	Sample source:mouse primary astrocytes | Genotype:Ent11 knockout | treatment:TNF𝝰/IL-1𝝰	Batch=B2; MS_ion Mode=positive; RAW_FILE_NAME=p_KO_4_Cytokine.mzML
SUBJECT_SAMPLE_FACTORS           	p_KO_5_Cytokine	p_KO_5_Cytokine	Sample source:mouse primary astrocytes | Genotype:Ent12 knockout | treatment:TNF𝝰/IL-1𝝰	Batch=B2; MS_ion Mode=positive; RAW_FILE_NAME=p_KO_5_Cytokine.mzML
SUBJECT_SAMPLE_FACTORS           	p_KO_6_Cytokine	p_KO_6_Cytokine	Sample source:mouse primary astrocytes | Genotype:Ent13 knockout | treatment:TNF𝝰/IL-1𝝰	Batch=B2; MS_ion Mode=positive; RAW_FILE_NAME=p_KO_6_Cytokine.mzML
SUBJECT_SAMPLE_FACTORS           	p_KO_7_Cytokine	p_KO_7_Cytokine	Sample source:mouse primary astrocytes | Genotype:Ent14 knockout | treatment:TNF𝝰/IL-1𝝰	Batch=B2; MS_ion Mode=positive; RAW_FILE_NAME=p_KO_7_Cytokine.mzML
SUBJECT_SAMPLE_FACTORS           	p_KO_1_CytokinePlusForo	p_KO_1_CytokinePlusForo	Sample source:mouse primary astrocytes | Genotype:Ent15 knockout | treatment:TNF𝝰/IL-1𝝰/Forodesine	Batch=B1; MS_ion Mode=positive; RAW_FILE_NAME=p_KO_1_CytokinePlusForo.mzML
SUBJECT_SAMPLE_FACTORS           	p_KO_2_CytokinePlusForo	p_KO_2_CytokinePlusForo	Sample source:mouse primary astrocytes | Genotype:Ent16 knockout | treatment:TNF𝝰/IL-2𝝰/Forodesine	Batch=B1; MS_ion Mode=positive; RAW_FILE_NAME=p_KO_2_CytokinePlusForo.mzML
SUBJECT_SAMPLE_FACTORS           	p_KO_3_CytokinePlusForo	p_KO_3_CytokinePlusForo	Sample source:mouse primary astrocytes | Genotype:Ent17 knockout | treatment:TNF𝝰/IL-3𝝰/Forodesine	Batch=B1; MS_ion Mode=positive; RAW_FILE_NAME=p_KO_3_CytokinePlusForo.mzML
SUBJECT_SAMPLE_FACTORS           	p_KO_4_CytokinePlusForo	p_KO_4_CytokinePlusForo	Sample source:mouse primary astrocytes | Genotype:Ent18 knockout | treatment:TNF𝝰/IL-4𝝰/Forodesine	Batch=B1; MS_ion Mode=positive; RAW_FILE_NAME=p_KO_4_CytokinePlusForo.mzML
SUBJECT_SAMPLE_FACTORS           	p_KO_5_CytokinePlusForo	p_KO_5_CytokinePlusForo	Sample source:mouse primary astrocytes | Genotype:Ent19 knockout | treatment:TNF𝝰/IL-5𝝰/Forodesine	Batch=B2; MS_ion Mode=positive; RAW_FILE_NAME=p_KO_5_CytokinePlusForo.mzML
SUBJECT_SAMPLE_FACTORS           	p_KO_6_CytokinePlusForo	p_KO_6_CytokinePlusForo	Sample source:mouse primary astrocytes | Genotype:Ent20 knockout | treatment:TNF𝝰/IL-6𝝰/Forodesine	Batch=B2; MS_ion Mode=positive; RAW_FILE_NAME=p_KO_6_CytokinePlusForo.mzML
SUBJECT_SAMPLE_FACTORS           	p_KO_7_CytokinePlusForo	p_KO_7_CytokinePlusForo	Sample source:mouse primary astrocytes | Genotype:Ent21 knockout | treatment:TNF𝝰/IL-7𝝰/Forodesine	Batch=B2; MS_ion Mode=positive; RAW_FILE_NAME=p_KO_7_CytokinePlusForo.mzML
#COLLECTION
CO:COLLECTION_SUMMARY            	Primary astrocytes were isolated from the brains of wild-type or ENT2-/- mice at
CO:COLLECTION_SUMMARY            	postnatal day 1 (P1). On DIV27, cells were stimulated with a cytokine cocktail,
CO:COLLECTION_SUMMARY            	and metabolites were extracted on DIV28 using 0.6N perchloric acid (PCA).
CO:COLLECTION_PROTOCOL_FILENAME  	culture_and_collection_protocol.pdf
CO:SAMPLE_TYPE                   	Astrocyte cells
#TREATMENT
TR:TREATMENT_SUMMARY             	Ent2+/+ and Ent2-/- primary astrocytes were treated on DIV27 with TNFα (30
TR:TREATMENT_SUMMARY             	ng/mL) and IL-1α (3 ng/mL) for 24 hours to induce activation. To inhibit PNP
TR:TREATMENT_SUMMARY             	activity, Forodesine (2 μM) was applied simultaneously with cytokine treatment.
TR:TREATMENT_PROTOCOL_FILENAME   	Treatment_protocol_24h.pdf
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	Primary astrocytes were washed, digested, and subjected to metabolite extraction
SP:SAMPLEPREP_SUMMARY            	using 0.6 N perchloric acid (PCA), followed by neutralization with K₂CO₃.
SP:SAMPLEPREP_SUMMARY            	The clarified supernatants were collected and used for metabolomic profiling.
SP:SAMPLEPREP_SUMMARY            	The remaining protein pellets were processed separately and retained for
SP:SAMPLEPREP_SUMMARY            	concentration measurement.
SP:SAMPLEPREP_PROTOCOL_FILENAME  	PCA_extraction.pdf
SP:EXTRACT_STORAGE               	-80℃
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_TYPE           	HILIC
CH:INSTRUMENT_NAME               	Agilent 1290 Infinity II UPLC system
CH:COLUMN_NAME                   	ACQUITY UPLC BEH Amide (100 x 2.1 mm, 1.7 μm)
CH:SOLVENT_A                     	100% Double-distilled Water; 15 mM ammonium acetate; 0.3% Ammonium hydroxide
CH:SOLVENT_B                     	90% Acetonitrile/10% Double-distilled Water; 15 mM ammonium acetate; 0.3% Ammonium hydroxide
CH:FLOW_GRADIENT                 	0–8 min, 90-50% B; 8–10 min, 50% B; 10–11 min, 50–90% B; 11–16 min,
CH:FLOW_GRADIENT                 	90% B. (amide column)
CH:FLOW_RATE                     	0.3 mL/min
CH:COLUMN_TEMPERATURE            	40℃
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
AN:ANALYSIS_PROTOCOL_FILE        	UPLC-MS-protocol.pdf
#MS
MS:INSTRUMENT_NAME               	Agilent 6545XT QTOF
MS:INSTRUMENT_TYPE               	QTOF
MS:MS_TYPE                       	ESI
MS:ION_MODE                      	POSITIVE
MS:MS_COMMENTS                   	Data were acquired in both positive and negative full-scan modes (m/z
MS:MS_COMMENTS                   	60–1500), processed using Agilent Qualitative Analysis 10.0 and Profinder
MS:MS_COMMENTS                   	B.10.00 software, and quantified based on peak area across treatments.
#MS_METABOLITE_DATA
MS_METABOLITE_DATA:UNITS	ion counts
MS_METABOLITE_DATA_START
Samples	p_WT_1_Ctrl	p_WT_2_Ctrl	p_WT_3_Ctrl	p_WT_4_Ctrl	p_WT_5_Ctrl	p_WT_6_Ctrl	p_KO_1_Ctrl	p_KO_2_Ctrl	p_KO_3_Ctrl	p_KO_4_Ctrl	p_KO_5_Ctrl	p_KO_6_Ctrl	p_WT_1_Cytokine	p_WT_2_Cytokine	p_WT_3_Cytokine	p_WT_4_Cytokine	p_WT_5_Cytokine	p_WT_6_Cytokine	p_WT_7_Cytokine	p_KO_1_Cytokine	p_KO_2_Cytokine	p_KO_3_Cytokine	p_KO_4_Cytokine	p_KO_5_Cytokine	p_KO_6_Cytokine	p_KO_7_Cytokine	p_WT_1_CytokinePlusForo	p_WT_2_CytokinePlusForo	p_WT_3_CytokinePlusForo	p_WT_4_CytokinePlusForo	p_WT_5_CytokinePlusForo	p_WT_6_CytokinePlusForo	p_WT_7_CytokinePlusForo	p_KO_1_CytokinePlusForo	p_KO_2_CytokinePlusForo	p_KO_3_CytokinePlusForo	p_KO_4_CytokinePlusForo	p_KO_5_CytokinePlusForo	p_KO_6_CytokinePlusForo	p_KO_7_CytokinePlusForo
Factors	Sample source:mouse primary astrocytes | Genotype:Wild-type | treatment:Vehicle	Sample source:mouse primary astrocytes | Genotype:Wild-type | treatment:Vehicle	Sample source:mouse primary astrocytes | Genotype:Wild-type | treatment:Vehicle	Sample source:mouse primary astrocytes | Genotype:Wild-type | treatment:Vehicle	Sample source:mouse primary astrocytes | Genotype:Wild-type | treatment:Vehicle	Sample source:mouse primary astrocytes | Genotype:Wild-type | treatment:Vehicle	Sample source:mouse primary astrocytes | Genotype:Ent2 knockout | treatment:Vehicle	Sample source:mouse primary astrocytes | Genotype:Ent3 knockout | treatment:Vehicle	Sample source:mouse primary astrocytes | Genotype:Ent4 knockout | treatment:Vehicle	Sample source:mouse primary astrocytes | Genotype:Ent5 knockout | treatment:Vehicle	Sample source:mouse primary astrocytes | Genotype:Ent6 knockout | treatment:Vehicle	Sample source:mouse primary astrocytes | Genotype:Ent7 knockout | treatment:Vehicle	Sample source:mouse primary astrocytes | Genotype:Wild-type | treatment:TNF𝝰/IL-1𝝰	Sample source:mouse primary astrocytes | Genotype:Wild-type | treatment:TNF𝝰/IL-1𝝰	Sample source:mouse primary astrocytes | Genotype:Wild-type | treatment:TNF𝝰/IL-1𝝰	Sample source:mouse primary astrocytes | Genotype:Wild-type | treatment:TNF𝝰/IL-1𝝰	Sample source:mouse primary astrocytes | Genotype:Wild-type | treatment:TNF𝝰/IL-1𝝰	Sample source:mouse primary astrocytes | Genotype:Wild-type | treatment:TNF𝝰/IL-1𝝰	Sample source:mouse primary astrocytes | Genotype:Wild-type | treatment:TNF𝝰/IL-1𝝰	Sample source:mouse primary astrocytes | Genotype:Ent8 knockout | treatment:TNF𝝰/IL-1𝝰	Sample source:mouse primary astrocytes | Genotype:Ent9 knockout | treatment:TNF𝝰/IL-1𝝰	Sample source:mouse primary astrocytes | Genotype:Ent10 knockout | treatment:TNF𝝰/IL-1𝝰	Sample source:mouse primary astrocytes | Genotype:Ent11 knockout | treatment:TNF𝝰/IL-1𝝰	Sample source:mouse primary astrocytes | Genotype:Ent12 knockout | treatment:TNF𝝰/IL-1𝝰	Sample source:mouse primary astrocytes | Genotype:Ent13 knockout | treatment:TNF𝝰/IL-1𝝰	Sample source:mouse primary astrocytes | Genotype:Ent14 knockout | treatment:TNF𝝰/IL-1𝝰	Sample source:mouse primary astrocytes | Genotype:Wild-type | treatment:TNF𝝰/IL-1𝝰/Forodesine	Sample source:mouse primary astrocytes | Genotype:Wild-type | treatment:TNF𝝰/IL-2𝝰/Forodesine	Sample source:mouse primary astrocytes | Genotype:Wild-type | treatment:TNF𝝰/IL-3𝝰/Forodesine	Sample source:mouse primary astrocytes | Genotype:Wild-type | treatment:TNF𝝰/IL-4𝝰/Forodesine	Sample source:mouse primary astrocytes | Genotype:Wild-type | treatment:TNF𝝰/IL-5𝝰/Forodesine	Sample source:mouse primary astrocytes | Genotype:Wild-type | treatment:TNF𝝰/IL-6𝝰/Forodesine	Sample source:mouse primary astrocytes | Genotype:Wild-type | treatment:TNF𝝰/IL-7𝝰/Forodesine	Sample source:mouse primary astrocytes | Genotype:Ent15 knockout | treatment:TNF𝝰/IL-1𝝰/Forodesine	Sample source:mouse primary astrocytes | Genotype:Ent16 knockout | treatment:TNF𝝰/IL-2𝝰/Forodesine	Sample source:mouse primary astrocytes | Genotype:Ent17 knockout | treatment:TNF𝝰/IL-3𝝰/Forodesine	Sample source:mouse primary astrocytes | Genotype:Ent18 knockout | treatment:TNF𝝰/IL-4𝝰/Forodesine	Sample source:mouse primary astrocytes | Genotype:Ent19 knockout | treatment:TNF𝝰/IL-5𝝰/Forodesine	Sample source:mouse primary astrocytes | Genotype:Ent20 knockout | treatment:TNF𝝰/IL-6𝝰/Forodesine	Sample source:mouse primary astrocytes | Genotype:Ent21 knockout | treatment:TNF𝝰/IL-7𝝰/Forodesine
Total protein	238.6	279.2	279.2	279.2	286	245.4	245.4	326.4	292.6	238.6	428	272.4	340	319.8	286	279.2	292.6	299.4	258.8	326.4	292.6	286	380.6	279.2	245.4	204.8	313	286	286	265.6	286	313	482	373.8	265.6	286	292.6	326.4	340	279.2
Fenclonine (IS)	89890	114691	98505	96122	82470	92774	94968	91274	90913	74102	65933	76185	113937	69617	112373	85947	89160	96719	89733	86418	65503	91192	67912	76304	72970	69061	107781	99622	86299	110413	86441	90160	84442	92540	94052	88405	85148	65011	66096	71078
Acetyl-CoA																																								
Adenine	6045045	5260451	6204603	8542392	9118546	7812516	6064514	5307835	6361569	8377334	12484783	11111987	6610439	6171527	5325796	8784115	6838612	8006772	7795448	7948946	11223329	8077037	10004015	9701032	8649979	9352425	4992849	6434826	4174415	6630004	9649181	9148978	7678355	4381183	4073349	3772020	5221655	5994059	6534384	7246007
Adenosine	70122	154564	90280	213796	154222	156008	119792	104432	132588	245139	253549	212922	76221	66938	97312	137130	154809	169840	99019	98090	115738	124264	152085	207675	105933	113307	93376	104960	68912	68657	255261	131850	108095	70254	75229	61727	52947	199190	99146	208600
ADP	45245	127927	59587	113317	111986	164897	137733	117425	197902	159733	205208	179106	101843	37984	80023	100932	74996	136143	102128	136706	72127	150195	135620	161271	143669	118421	67059	56441	52768	107513	157100	116982	96766	117957	139858	136924	113562	62605	73471	79205
AMP	66531	114706	62305	73327	68923	77447	86025	87108	100937	79623	104376	75870	76583	41195	56382	58902	51538	62688	55927	72891	53986	77355	59002	71002	64694	67607	56077	48451	43572	61564	66343	50750	44738	59350	63316	77482	92039	50660	50346	58800
ATP	239010	258189	257582	350590	333034	346007	313193	274804	299556	339452	335970	318727	266772	233671	239744	269718	255291	328756	306450	293154	313620	334361	339707	344419	321075	293314	171248	242890	203934	271316	313617	306113	268301	257488	237147	252046	256725	281816	299473	306682
CDP																																								
CMP	880	1234	924	978	502	955	1509	1092	1702	1194	1555	1303	854	698	1099	1002	624	893	676	1856	1328	2643	1585	1668	1292	1511	756	991	882	1565	1687	887	1249	1697	1489	1800	1929	1128	1134	1312
CTP	4795	5389	5643	7357	7111	8094	7812	6291	7061	8180	8951	8607	6752	5867	6310	6854	6223	8498	8163	12967	14926	13773	13949	15769	14419	11657	7879	10849	7916	11443	14569	13972	11824	13264	10086	11090	9519	12011	13625	14747
Cytidine																																								
Cytosine	9418	7319	6537	15217	10671	18594	9946	6469	8999	14158	26459	26449	9368	7197	7335	8697	11022	16529	13080	8383	14348	11251	18126	19711	17712	15287	5502	4385	6191	8315	14605	15150	12481	7984	8346	8326	5892	12131	13233	17346
GDP	4087	5955	4076	6586	6088	7237	5869	5391	6544	6002	7375	7087	5379	3655	5135	5543	4958	6508	5686	6353	4310	6472	6207	6792	6272	6359	5305	4462	4375	5605	7365	6386	5952	6044	5553	5931	5761	4766	5003	5789
GMP																																								
GSH																																								
GSNO																																								
GSSG	669321	661762	735954	987767	880750	896081	1066445	934419	1079493	964163	986188	953450	924660	810380	813897	828735	806710	1135215	1049000	1153423	1255405	1418183	1225249	1333941	1186362	994786	625927	830450	735803	1092400	1182207	1089961	956960	1049909	960685	1148870	1181685	946168	992382	1124261
GTP	17980	19233	19965	24383	23897	26101	26708	21884	23778	25938	26120	24577	22612	19342	19314	22775	19674	26393	24425	28568	30997	32584	30268	32002	28340	23601	16501	25110	18095	27092	28171	26513	25077	25216	20604	23558	23039	25033	27735	27507
Guanine	91233	109006	92607	367501	339380	264460	145346	116384	150347	304534	755118	657002	122120	69115	103185	284585	219706	272935	238660	149907	129220	143959	366859	328962	345819	375049	121465	102754	67921	124266	414559	353859	275587	112226	112396	118988	118279	322121	310779	414613
Guanosine																																								
Malonyl-CoA																																								
NAD+	29470	27113	16017	40386	30793	35306	42972	30347	38091	37796	66713	46342	24564	13789	21633	23339	16570	39722	29163	26868	43314	35604	37038	48893	30403	33911	17746	26488	15301	33041	58341	43927	33594	35026	36837	54513	33301	66106	68073	73239
NADH																																								
NADP+	3878	3182	2465	4076	4227	3778	3730	3130	4043	4189	6653	4877	3551	2285	4518	3623	3106	5161	3840	2462	4725	3264	3444	3778	2607	3148	3600	3333	2687	4021	4417	4271	4075	2799	3092	4724	2287	4847	5447	5428
NADPH																																								
Succinyl-CoA																																								
Adenosine diphosphate ribose	1000	658	515	801	520	617	859	774	883	782	1118	916	1102	984	520	791	634	673	688	964	1477	1253	895	1120	774	912	575	828	599	909	909	804	834	1208	919	868	842	850	924	1078
GABA																																								
Nicotinamide riboside																																								
Nicotinic acid riboside																																								
MS_METABOLITE_DATA_END
#METABOLITES
METABOLITES_START
metabolite_name	Formula	Mass	RT	CAS ID
Total protein
Fenclonine (IS)	C9 H10 Cl N O2	199.039	1.9	14173-39-8
Acetyl-CoA	C23 H38 N7 O17 P3 S	809.126	4.6	72-89-9
Adenine	C5 H5 N5	135.055	1.7	73-24-5
Adenosine	C10 H13 N5 O4	267.0962	1.8	58-61-7
ADP	C10 H15 N5 O10 P2	427.029	5.5	58-64-0
AMP	C10 H14 N5 O7 P	347.063	5.0	61-19-8
ATP	C10 H16 N5 O13 P3	506.996	5.9	56-65-5
CDP	C9 H15 N3 O11 P2	403.018	5.6	63-38-7
CMP	C9 H14 N3 O8 P	323.051	5.5	63-37-6
CTP	C9 H16 N3 O14 P3	482.983	6.4	65-47-4
Cytidine	C9 H13 N3 O5	243.086	2.2	65-46-3
Cytosine	C4 H5 N3 O	111.044	2.1	71-30-7
GDP	C10 H15 N5 O11 P2	443.023	6.1	146-91-8
GMP	C10 H14 N5 O8 P	363.058	5.4	85-32-5
GSH	C10 H17 N3 O6 S	307.084	4.4	70-18-8
GSNO	C10 H16 N4 O7 S	336.074	4.0	57564-91-7
GSSG	C20 H32 N6 O12 S2	612.153	6.2	27025-41-8
GTP	C10 H16 N5 O14 P3	522.989	6.5	86-01-1
Guanine	C5 H5 N5 O	151.050	2.3	73-40-5
Guanosine	C10 H13 N5 O5	283.092	2.5	118-00-3
Malonyl-CoA	C24 H38 N7 O19 P3 S	853.116	5.6	524-14-1
NAD+	C21 H28 N7 O14 P2	664.115	5.1	53-84-9
NADH	C21 H29 N7 O14 P2	665.125	4.3	58-68-4
NADP+	C21 H29 N7 O17 P3	744.081	6.2	53-59-8
NADPH	C21 H30 N7 O17 P3	745.091	5.6	53-57-6
Succinyl-CoA	C25 H40 N7 O19 P3 S	867.131	5.5	604-98-8
Adenosine diphosphate ribose	C15 H23 N5 O14 P2	559.070	4.9
GABA	C4 H9 N O2	103.063	4.1	56-12-2
Nicotinamide riboside	C11 H15 N2 O5	255.098	4.1
Nicotinic acid riboside	C11 H13 N O6	255.074	3.4
METABOLITES_END
#END