#METABOLOMICS WORKBENCH Xuanli97_20250904_053250 DATATRACK_ID:6380 STUDY_ID:ST004175 ANALYSIS_ID:AN006930 PROJECT_ID:PR002634 VERSION 1 CREATED_ON September 9, 2025, 2:04 pm #PROJECT PR:PROJECT_TITLE Directed Rescue Strategy for Enhanced Implant Osteointegration in Aged Rats PR:PROJECT_SUMMARY Senolytics, which involve the removal of senescent cells from tissues, have PR:PROJECT_SUMMARY emerged as one of the most promising strategies for treating age-related PR:PROJECT_SUMMARY degenerative diseases. In the context of orthopedic treatment, the elimination PR:PROJECT_SUMMARY of senescent cells can also enhance to the osteointegration of implants in PR:PROJECT_SUMMARY elderly patients. However, achieving specific clearance of senescent cells PR:PROJECT_SUMMARY without adversely affecting the function of normal cell remains challenging. To PR:PROJECT_SUMMARY overcome these challenges, we developed a novel implant surface modification PR:PROJECT_SUMMARY technique to achieve specific clearance of locally senescent cells by modulating PR:PROJECT_SUMMARY their metabolism. Our technique also involved modifying implants with BPTES, a PR:PROJECT_SUMMARY glutaminase 1 (GLS1) inhibitor, through π-π stacking with dopamine. This PR:PROJECT_SUMMARY modification effectively induced apoptosis in senescent mesenchymal stem cells PR:PROJECT_SUMMARY (MSCs) through extensive inhibition of GLS1. This effect was attributed to PR:PROJECT_SUMMARY intracellular acidosis resulting from the suppression of glutaminolysis in PR:PROJECT_SUMMARY senescent MSCs. Simultaneously, poly(γ-glutamate) (PGA), modified by a PR:PROJECT_SUMMARY layer-by-layer method, served as a high-density carbon source coating, PR:PROJECT_SUMMARY continuously supporting glutamine metabolism in MSCs without ammonia production. PR:PROJECT_SUMMARY Targeted metabolic analysis revealed that the modified titanium implants PR:PROJECT_SUMMARY significantly altered the metabolic profile of MSCs, enhancing glutamine PR:PROJECT_SUMMARY metabolism, the pentose phosphate pathway (PPP), aerobic glycolysis, and fatty PR:PROJECT_SUMMARY acid oxidation (FAO), which collectively stimulated osteogenic differentiation. PR:PROJECT_SUMMARY In vivo experiments showed that the surface modification significantly reduced PR:PROJECT_SUMMARY the senescence level around implants and promoted osteointegration in aged rats. PR:PROJECT_SUMMARY These findings offer promising insights into the design and application of PR:PROJECT_SUMMARY orthopedic implants for elderly patients. PR:INSTITUTE Chongqing University PR:LABORATORY College of Bioengineering, Chongqing University PR:LAST_NAME Li PR:FIRST_NAME Xuan PR:ADDRESS 174 Shazheng St., Shapingba, Chongqing, 400044, China PR:EMAIL 201919021061@cqu.edu.cn PR:PHONE 15310893373 PR:FUNDING_SOURCE The National Natural Science Foundation of China (Nos. 52333011, 52021004, PR:FUNDING_SOURCE 21734002 and 32171327), State Key Project of Research and Development PR:FUNDING_SOURCE (2022YFB3804400), and the Natural Science Foundation of Chongqing (No. PR:FUNDING_SOURCE cstc2021jcyj-cxttX0002) #STUDY ST:STUDY_TITLE The influence of implants on the amino acid metabolism of mesenchymal stem cells ST:STUDY_TITLE (MSCs) ST:STUDY_SUMMARY To determine whether the modified titanium implant can regulate the glutamine ST:STUDY_SUMMARY metabolism of mesenchymal stem cells (MSC), the amino acid metabolism levels of ST:STUDY_SUMMARY each group were analyzed. ST:INSTITUTE Chongqing University ST:LABORATORY College of Bioengineering, Chongqing University ST:LAST_NAME Li ST:FIRST_NAME Xuan ST:ADDRESS 174 Shazheng St., Shapingba,, Chongqing, 400044, China ST:EMAIL 201919021061@cqu.edu.cn ST:PHONE 15310893373 #SUBJECT SU:SUBJECT_TYPE Cultured cells SU:SUBJECT_SPECIES Rattus norvegicus SU:GENOTYPE_STRAIN wild type SU:AGE_OR_AGE_RANGE 4 weeks old SU:GENDER Male SU:CELL_BIOSOURCE_OR_SUPPLIER Primary cells from Rattus norvegicus SU:CELL_PRIMARY_IMMORTALIZED Non-immortalized cells SU:CELL_PASSAGE_NUMBER Passage 3 SU:CELL_COUNTS 0.5*10^7 cells #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data SUBJECT_SAMPLE_FACTORS - R1-1 Sample source:MSCs Primary cells | Culture substrate:Ti RAW_FILE_NAME(raw data file)=Hd12LX_R1_1.mzML SUBJECT_SAMPLE_FACTORS - R1-2 Sample source:MSCs Primary cells | Culture substrate:Ti RAW_FILE_NAME(raw data file)=Hd12LX_R1_2.mzML SUBJECT_SAMPLE_FACTORS - R1-3 Sample source:MSCs Primary cells | Culture substrate:Ti RAW_FILE_NAME(raw data file)=Hd12LX_R1_3.mzML SUBJECT_SAMPLE_FACTORS - R1-4 Sample source:MSCs Primary cells | Culture substrate:Ti RAW_FILE_NAME(raw data file)=Hd12LX_R1_4.mzML SUBJECT_SAMPLE_FACTORS - R1-5 Sample source:MSCs Primary cells | Culture substrate:Ti RAW_FILE_NAME(raw data file)=Hd12LX_R1_5.mzML SUBJECT_SAMPLE_FACTORS - R2-1 Sample source:MSCs Primary cells | Culture substrate:MT RAW_FILE_NAME(raw data file)=Hd12LX_R2_1.mzML SUBJECT_SAMPLE_FACTORS - R2-2 Sample source:MSCs Primary cells | Culture substrate:MT RAW_FILE_NAME(raw data file)=Hd12LX_R2_2.mzML SUBJECT_SAMPLE_FACTORS - R2-3 Sample source:MSCs Primary cells | Culture substrate:MT RAW_FILE_NAME(raw data file)=Hd12LX_R2_3.mzML SUBJECT_SAMPLE_FACTORS - R2-4 Sample source:MSCs Primary cells | Culture substrate:MT RAW_FILE_NAME(raw data file)=Hd12LX_R2_4.mzML SUBJECT_SAMPLE_FACTORS - R2-5 Sample source:MSCs Primary cells | Culture substrate:MT RAW_FILE_NAME(raw data file)=Hd12LX_R2_5.mzML SUBJECT_SAMPLE_FACTORS - R3-1 Sample source:MSCs Primary cells | Culture substrate:BPTES@MNT RAW_FILE_NAME(raw data file)=Hd12LX_R3_1.mzML SUBJECT_SAMPLE_FACTORS - R3-2 Sample source:MSCs Primary cells | Culture substrate:BPTES@MNT RAW_FILE_NAME(raw data file)=Hd12LX_R3_2.mzML SUBJECT_SAMPLE_FACTORS - R3-3 Sample source:MSCs Primary cells | Culture substrate:BPTES@MNT RAW_FILE_NAME(raw data file)=Hd12LX_R3_3.mzML SUBJECT_SAMPLE_FACTORS - R3-4 Sample source:MSCs Primary cells | Culture substrate:BPTES@MNT RAW_FILE_NAME(raw data file)=Hd12LX_R3_4.mzML SUBJECT_SAMPLE_FACTORS - R3-5 Sample source:MSCs Primary cells | Culture substrate:BPTES@MNT RAW_FILE_NAME(raw data file)=Hd12LX_R3_5.mzML SUBJECT_SAMPLE_FACTORS - R4-1 Sample source:MSCs Primary cells | Culture substrate:BPTES@MNT/PGA RAW_FILE_NAME(raw data file)=Hd12LX_R4_1.mzML SUBJECT_SAMPLE_FACTORS - R4-2 Sample source:MSCs Primary cells | Culture substrate:BPTES@MNT/PGA RAW_FILE_NAME(raw data file)=Hd12LX_R4_2.mzML SUBJECT_SAMPLE_FACTORS - R4-3 Sample source:MSCs Primary cells | Culture substrate:BPTES@MNT/PGA RAW_FILE_NAME(raw data file)=Hd12LX_R4_3.mzML SUBJECT_SAMPLE_FACTORS - R4-4 Sample source:MSCs Primary cells | Culture substrate:BPTES@MNT/PGA RAW_FILE_NAME(raw data file)=Hd12LX_R4_4.mzML SUBJECT_SAMPLE_FACTORS - R4-5 Sample source:MSCs Primary cells | Culture substrate:BPTES@MNT/PGA RAW_FILE_NAME(raw data file)=Hd12LX_R4_5.mzML #COLLECTION CO:COLLECTION_SUMMARY MSCs were seeded onto different samples and cultured at 37°C under 5% CO₂. CO:COLLECTION_SUMMARY The culture medium was replaced with fresh medium every two days. After 7 days CO:COLLECTION_SUMMARY of culture, all cells were collected. CO:SAMPLE_TYPE Mesenchymal stem cells CO:COLLECTION_METHOD Cells were harvested via trypsin digestion. The cells were centrifuged at 500 × CO:COLLECTION_METHOD g and collected into a 15 mL centrifuge tube. After discarding the supernatant, CO:COLLECTION_METHOD the cell pellet was resuspended in 10 mL of pre-cooled PBS and centrifuged for CO:COLLECTION_METHOD washing (repeated 3 times). The cell pellet was then resuspended in 1 mL PBS, CO:COLLECTION_METHOD and the cell concentration was accurately quantified. 0.5 × 10^7 cells were CO:COLLECTION_METHOD transferred to a 1.5 mL centrifuge tube, centrifuged at 500 × g for 5 min, and CO:COLLECTION_METHOD the supernatant was completely removed. The cell pellet was flash-frozen in CO:COLLECTION_METHOD liquid nitrogen and stored at −80°C. CO:COLLECTION_LOCATION Room 416, Cell Laboratory, School of Bioengineering, Chongqing University CO:COLLECTION_FREQUENCY 5 times CO:COLLECTION_DURATION 1 h CO:VOLUMEORAMOUNT_COLLECTED 0.5 * 10^7 cells CO:STORAGE_CONDITIONS -80℃ CO:COLLECTION_VIALS 15 mL centrifuge tube CO:STORAGE_VIALS 1.5 mL centrifuge tube CO:COLLECTION_TUBE_TEMP 4 #TREATMENT TR:TREATMENT_SUMMARY Befere the collection, the cells were cultured on the surface of different TR:TREATMENT_SUMMARY substrates for 7 days as a treatment step. Ti means titanium foils which were TR:TREATMENT_SUMMARY purchased from Alfa Aesar Co. (Tianjin, China). MT was fabricated from Ti via TR:TREATMENT_SUMMARY acid etching. Briefly, the titanium was sequentially treated with 3 wt% TR:TREATMENT_SUMMARY hydrofluoric acid at room temperature for 2 min and then immersed in 66 wt% TR:TREATMENT_SUMMARY sulfuric acid at 80 °C for 5 min. The obtained MT was rinsed thoroughly by TR:TREATMENT_SUMMARY ultrasonic. Subsequently, MT was immersed in 3 % APTES solution (toluene as TR:TREATMENT_SUMMARY solvent) for 24 h, and then washed thoroughly for further use. BPTES@MNT was TR:TREATMENT_SUMMARY obtained by one-step polymerization of dopamine. Briefly, MT-NH2 was placed in a TR:TREATMENT_SUMMARY mixed solution (VTris-HCl buffer (10 mM, pH 8.5): VDMSO = 1:1) containing TR:TREATMENT_SUMMARY dopamine (1 mg/mL), CaCl2 (0.05 mg/mL), BPTES (0.05 mg/mL) and 1 % APS. The TR:TREATMENT_SUMMARY reaction mixture was under continuous shaking (40 rpm) at room temperature TR:TREATMENT_SUMMARY overnight and avoid light. The obtained samples were rinsed thoroughly with DI TR:TREATMENT_SUMMARY water. Subsequently, PGA coating was modified on the substrate of BPTES@MNT by TR:TREATMENT_SUMMARY layer-by-layer method. In short, the samples were alternately immersed into Chi TR:TREATMENT_SUMMARY (0.5 w/v%) and PGA (1 w/v%) solution (two repeats). The samples were sterilized TR:TREATMENT_SUMMARY with ultraviolet irradiation or prepared under an aseptic environment for TR:TREATMENT_SUMMARY subsequent in vitro and in vivo experiments. TR:TREATMENT different culture substrates TR:TREATMENT_COMPOUND Ti (R1), MT(R2), BPTES@MNT(R3), and BPTES@MNT/PGA(R4) TR:TREATMENT_ROUTE the cells were seeded in the different substrate (The sterile titanium substrate TR:TREATMENT_ROUTE (different groups) was placed in a petri dish to fully cover the base) TR:TREATMENT_VEHICLE Ti (R1) TR:CELL_STORAGE Primary cells TR:CELL_GROWTH_CONTAINER Cell Culture Dishes (Φ100mm) TR:CELL_GROWTH_CONFIG spindle-shaped or elongated TR:CELL_INOC_PROC The sterile titanium substrate (different groups) was placed in a petri dish to TR:CELL_INOC_PROC fully cover the base. Third-passage MSCs (0.5*10^6) were seeded on the titanium TR:CELL_INOC_PROC surface, followed by the addition of fresh culture medium. The cells were TR:CELL_INOC_PROC cultured at 37°C under 5% CO₂ atmosphere, and the medium was replaced with TR:CELL_INOC_PROC fresh medium every two days. TR:CELL_MEDIA DMEM Medium(Low Glucose, with 10 % fetal bovine serum) TR:CELL_ENVIR_COND incubated at 37 °C under 5 % CO2 atmosphere TR:CELL_HARVESTING trypsin digestion TR:CELL_PCT_CONFLUENCE 90 % TR:CELL_MEDIA_LASTCHANGED one day before collection #SAMPLEPREP SP:SAMPLEPREP_SUMMARY In this experiment, a certain mass of the sample was precisely measured. Then, SP:SAMPLEPREP_SUMMARY the sample was added to the extraction solution (tissues, cells and other SP:SAMPLEPREP_SUMMARY samples need to be ground first) in a low-temperature environment for metabolite SP:SAMPLEPREP_SUMMARY extraction treatment. Standard solutions of different concentrations were SP:SAMPLEPREP_SUMMARY prepared. The standard solutions and the sample to be tested were subjected to SP:SAMPLEPREP_SUMMARY LC-MS analysis under the same conditions. The detailed steps are as follows: SP:SAMPLEPREP_SUMMARY 0.5*10^7 cells were combined with 4 μL of internal standard solution SP:SAMPLEPREP_SUMMARY (containing 100 μg/mL L-Trp-D5, L-Gln-D5, and L-Lys-D4), 216 μL purified SP:SAMPLEPREP_SUMMARY water and 25 μL of 0.15 % DOC.The mixed solution was sonicated (40 kHz) for SP:SAMPLEPREP_SUMMARY 10 min at 5 °C, and then 5 μL of trichloroacetic acid (10 M) were added. SP:SAMPLEPREP_SUMMARY Before centrifugation, the sample was stored at -20 °C for 10 min. Then the SP:SAMPLEPREP_SUMMARY sample was centrifuged at 14000 g for 10 min at 4 °C, and 50 μL of SP:SAMPLEPREP_SUMMARY supernatant was collected. 350 μL purified water were added, and then the SP:SAMPLEPREP_SUMMARY mixed solution was filtrated by a PTFE filter (0.2 μm, biotage) for further SP:SAMPLEPREP_SUMMARY analysis. SP:PROCESSING_STORAGE_CONDITIONS 4℃ SP:EXTRACT_STORAGE 4℃ #CHROMATOGRAPHY CH:CHROMATOGRAPHY_SUMMARY In this experiment, LC-ESI-MS/MS (UHPLC-Qtrap) was used to conduct qualitative CH:CHROMATOGRAPHY_SUMMARY and quantitative detection of the target substances in the samples. The specific CH:CHROMATOGRAPHY_SUMMARY parameters are as follows: Chromatographic conditions: AdvanceBio MS Spent Media CH:CHROMATOGRAPHY_SUMMARY (2.1 * 50mm, 2.7 µm), column temperature 40℃, injection volume 1 μL. Mobile CH:CHROMATOGRAPHY_SUMMARY phase A (0.1% formic acid 10mM ammonium formate 95% water solution), mobile CH:CHROMATOGRAPHY_SUMMARY phase B (0.1% formic acid 10mM ammonium formate 95% acetonitrile solution). CH:CHROMATOGRAPHY_TYPE HILIC CH:INSTRUMENT_NAME ACQUITY UPLC CH:COLUMN_NAME Agilent AdvanceBio MS Spent Media (50 x 2.1mm, 2.7um) CH:SOLVENT_A 100% water; 0.1% formic acid; 10 mM ammonium formate CH:SOLVENT_B 95% acetonitrile/5% water0.1% formic acid; 10 mM ammonium formate CH:FLOW_GRADIENT 0.0min 10% A; 3min 40% A; 4min 40% A; 4.1min 10% A; 6min 10% A CH:FLOW_RATE 0.5ml/min CH:COLUMN_TEMPERATURE 40 CH:INTERNAL_STANDARD 100 μg/mL L-Trp-D5, L-Gln-D5 and L-Lys-D4), 216 μL purified water (fisher) CH:INTERNAL_STANDARD and 25 μL of 0.15 % DOC #ANALYSIS AN:ANALYSIS_TYPE MS #MS MS:INSTRUMENT_NAME ABI Sciex 6500+ QTrap MS:INSTRUMENT_TYPE Triple quadrupole MS:MS_TYPE ESI MS:ION_MODE POSITIVE MS:MS_COMMENTS QTRAP 6500+ (SCIEX) was used to analyze and the system was set in positive mode. MS:MS_COMMENTS The temperature was set to 550 °C, curtain gas was maintained at 35 psi, MS:MS_COMMENTS collision gas was set to medium, and both ion source gas 1 and 2 were set to MS:MS_COMMENTS 50 psi. Ion-spray voltage was set to 5500 V. In the AB Sciex quantitative MS:MS_COMMENTS software OS, the default parameters are used to automatically identify and MS:MS_COMMENTS integrate each ion fragment, with manual checks as an auxiliary. A linear MS:MS_COMMENTS regression standard curve is plotted with the mass spectrometry peak area of the MS:MS_COMMENTS analyte as the ordinate and the analyte concentration as the abscissa. Sample MS:MS_COMMENTS concentration calculation: The mass spectrometry peak area of the analyte in the MS:MS_COMMENTS sample is substituted into the linear equation to calculate the concentration MS:MS_COMMENTS result. MS:COLLISION_GAS Medium #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS ng/ml MS_METABOLITE_DATA_START Samples R1-1 R1-2 R1-3 R1-4 R1-5 R2-1 R2-2 R2-3 R2-4 R2-5 R3-1 R3-2 R3-3 R3-4 R3-5 R4-1 R4-2 R4-3 R4-4 R4-5 Factors Sample source:MSCs Primary cells | Culture substrate:Ti Sample source:MSCs Primary cells | Culture substrate:Ti Sample source:MSCs Primary cells | Culture substrate:Ti Sample source:MSCs Primary cells | Culture substrate:Ti Sample source:MSCs Primary cells | Culture substrate:Ti Sample source:MSCs Primary cells | Culture substrate:MT Sample source:MSCs Primary cells | Culture substrate:MT Sample source:MSCs Primary cells | Culture substrate:MT Sample source:MSCs Primary cells | Culture substrate:MT Sample source:MSCs Primary cells | Culture substrate:MT Sample source:MSCs Primary cells | Culture substrate:BPTES@MNT Sample source:MSCs Primary cells | Culture substrate:BPTES@MNT Sample source:MSCs Primary cells | Culture substrate:BPTES@MNT Sample source:MSCs Primary cells | Culture substrate:BPTES@MNT Sample source:MSCs Primary cells | Culture substrate:BPTES@MNT Sample source:MSCs Primary cells | Culture substrate:BPTES@MNT/PGA Sample source:MSCs Primary cells | Culture substrate:BPTES@MNT/PGA Sample source:MSCs Primary cells | Culture substrate:BPTES@MNT/PGA Sample source:MSCs Primary cells | Culture substrate:BPTES@MNT/PGA Sample source:MSCs Primary cells | Culture substrate:BPTES@MNT/PGA L-Alanine 503.73345 542.5671 533.36494 602.19652 458.272 407.13411 464.16726 442.64501 503.99835 550.47999 640.80051 624.63223 664.78142 676.14655 683.12191 775.21309 783.67694 736.89232 781.95225 716.75219 L-(+)-Arginine 747.61701 716.74484 705.29315 913.9677 618.18596 421.24688 457.22851 448.9397 534.41749 981.47159 1050.29512 927.73356 1072.18849 1002.65625 1216.23578 1244.05014 1215.64875 1037.09011 1086.8357 1023.75226 L-Asparagine Anhydrous 307.23493 334.83811 291.74472 364.07675 289.88148 210.61032 279.73402 250.12392 324.46879 361.31174 389.32489 388.24549 433.20044 446.3511 449.62071 494.21163 504.02451 485.82815 481.31701 455.26438 L-AsparticAcid 740.98068 637.56748 631.45046 573.79535 547.99613 489.3129 478.89094 550.78524 539.90837 593.26238 685.19861 640.57597 680.69797 672.69512 614.95347 827.94115 890.79731 810.79548 823.05527 818.03479 L-Glutamine 1594.281 2122.88787 2214.43754 2363.90644 1911.18027 1554.31286 1686.934 1722.44501 2087.91544 2256.11576 2438.61266 2938.80925 2824.09803 3018.74 3011.12669 4565.23623 4500.32638 4194.73241 4361.1269 4289.93407 L-GlutamicAcid 649.4208 628.13025 637.09955 676.65553 557.92862 662.62534 738.88345 806.28213 847.65437 871.16902 953.77845 984.90019 1022.86634 1000.85469 1007.32509 1595.47719 1734.04741 1531.38204 1594.32264 1581.13196 Glycine 522.6724 421.05392 460.23851 598.88041 490.46311 466.6627 414.36065 422.75777 566.0786 446.87837 620.78438 621.1977 577.63062 697.28075 600.20282 732.53784 857.18974 702.03818 732.5694 653.47998 L-Histidine 588.1471 402.85062 336.9555 331.4183 275.778 196.64828 208.7529 221.87499 222.17967 231.77203 348.23983 327.22331 367.22254 356.0082 345.55261 406.03334 407.95508 361.37705 378.3088 381.59686 L-Isoleucine 286.95373 302.59484 270.52435 352.1422 269.87337 168.74627 206.18313 215.16314 245.33895 302.28447 332.61634 319.67325 360.04918 385.14642 398.67638 376.62401 392.3326 371.04865 363.04561 336.53495 L-Cysteine 82.41944 58.91226 83.31478 81.71616 69.90151 69.33816 76.11059 84.03346 66.84812 55.45649 80.89996 87.7004 96.47734 82.84621 83.25244 56.84849 73.04365 55.40352 68.05245 38.39095 L-Leucine 742.04133 786.33718 700.70472 846.98181 678.49453 453.93342 592.04693 543.95637 677.49924 728.1298 908.80759 863.17112 899.8456 1040.67528 1024.08966 1079.879 1089.2592 1045.20839 1058.50665 923.45566 L-Hydroxyproline 3.86221 2.73681 2.73207 2.96837 2.13708 5.10444 5.84252 4.45602 5.08342 5.44777 6.28637 6.16216 6.08239 5.14971 6.36817 8.63671 6.57908 6.08039 6.75248 7.56436 L-Tryptophan 74.43673 92.81601 94.55067 109.52571 87.13955 66.77622 80.09183 72.5057 91.46356 112.57166 114.03314 122.43415 131.11933 152.54329 141.50334 154.91373 147.932 147.45345 146.91392 130.19617 L-(+)-Lysine 946.82357 1265.43261 1196.99925 1327.22421 1130.06621 830.46923 905.39703 870.17889 931.70962 1147.95432 1336.93741 1337.03316 1489.09127 1470.51778 1454.18445 1564.87485 1762.92343 1664.50629 1666.58715 1517.62479 L-Methionine 215.19008 208.56869 220.34083 238.27451 198.53129 142.44205 177.22849 172.97023 201.36127 232.64636 252.38422 243.07132 269.37371 289.51385 295.82226 306.6741 305.52459 286.05614 286.46738 255.91612 L-Phenylalanine 296.18568 308.36921 301.75644 352.92593 264.2173 202.7297 251.04355 241.87426 291.71238 317.84681 346.28798 349.34228 381.90278 402.75037 416.6518 414.64591 410.40996 420.98718 439.64708 378.86597 L-Proline 478.47015 499.04804 497.87935 543.27643 454.23602 362.36828 411.13526 405.68833 441.21292 494.82386 571.42646 552.5165 581.1293 580.37365 567.00264 675.41401 691.64612 649.6753 651.71019 619.48467 L-Serine 773.31893 732.83901 694.32089 736.86647 629.33503 474.9127 580.69467 551.60721 627.99637 694.79816 828.02695 798.42194 838.79571 904.37612 886.77325 960.1191 1043.95748 915.36448 953.5133 903.4553 L-(-)-Threonine 343.66927 341.43286 330.15521 397.5309 310.01879 249.58219 280.87578 276.27934 324.56809 357.02963 398.96025 400.19223 410.44065 445.88777 423.75241 499.11427 533.79051 507.29671 495.42362 480.34773 L-(-)-Tyrosine 448.84111 443.84967 413.6689 476.13482 395.1469 311.02134 367.1202 373.21042 418.8385 479.41776 507.3884 495.46458 533.11797 579.85273 564.78565 625.00572 619.22889 572.56248 608.31591 538.04392 L-Valine 417.81332 426.25776 415.49104 473.56662 360.07532 240.12893 305.9803 298.21574 355.49819 424.93919 457.88573 447.21118 507.11601 543.34311 527.34026 541.04824 531.55447 515.34553 527.72095 485.77329 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name L-Alanine L-(+)-Arginine L-Asparagine Anhydrous L-AsparticAcid L-Glutamine L-GlutamicAcid Glycine L-Histidine L-Isoleucine L-Cysteine L-Leucine L-Hydroxyproline L-Tryptophan L-(+)-Lysine L-Methionine L-Phenylalanine L-Proline L-Serine L-(-)-Threonine L-(-)-Tyrosine L-Valine METABOLITES_END #END