#METABOLOMICS WORKBENCH msholola_20250902_153403 DATATRACK_ID:6368 STUDY_ID:ST004178 ANALYSIS_ID:AN006936 PROJECT_ID:PR002635 VERSION 1 CREATED_ON September 8, 2025, 9:40 pm #PROJECT PR:PROJECT_TITLE Tomato-Soy Juice Reduces Inflammation and Modulates Urinary Metabolome in Adults PR:PROJECT_TITLE with Obesity PR:PROJECT_SUMMARY Scope: Chronic, low-grade inflammation is a hallmark of many noncommunicable PR:PROJECT_SUMMARY diseases, including obesity. Diets enriched with tomatoes and soy have been PR:PROJECT_SUMMARY associated with better health outcomes in inflammation-related illnesses, with PR:PROJECT_SUMMARY lycopene and isoflavones considered key bioactive components, respectively. On PR:PROJECT_SUMMARY the basis that whole food combinations may exert greater effects than isolated PR:PROJECT_SUMMARY phytochemicals, we examine the anti-inflammatory and metabolic effects of PR:PROJECT_SUMMARY tomato-soy juice compared to a low carotenoid tomato juice control in obesity. PR:PROJECT_SUMMARY Methods and results: In a randomized, crossover trial, 12 healthy adults with PR:PROJECT_SUMMARY obesity were provided either tomato-soy juice (54 mg lycopene/d, 189.9 mg PR:PROJECT_SUMMARY isoflavones/d) or a low carotenoid tomato juice (no isoflavones) daily for 4 PR:PROJECT_SUMMARY weeks, then crossed over to the other treatment following a washout period. PR:PROJECT_SUMMARY Plasma carotenoids, cytokines, and the urine metabolome were measured pre- and PR:PROJECT_SUMMARY post-interventions. Plasma lycopene significantly increased by 2.48-fold after PR:PROJECT_SUMMARY tomato-soy intake. IL-5, IL-12p70, and GM-CSF significantly decreased (P < PR:PROJECT_SUMMARY 0.05), and TNF-alpha trended downward (P = 0.052) following tomato-soy. Soy PR:PROJECT_SUMMARY isoflavones and their metabolites primarily distinguished post-tomato-soy urine PR:PROJECT_SUMMARY profiles. Both interventions induced some shared metabolomic changes in the PR:PROJECT_SUMMARY urine, indicating tomato-driven effects independent of lycopene. Conclusion: PR:PROJECT_SUMMARY Tomato-soy intake reduced some pro-inflammatory cytokines and altered the urine PR:PROJECT_SUMMARY metabolomic profile in adults with obesity, supporting future studies using this PR:PROJECT_SUMMARY functional food product for other inflammation-related conditions. PR:INSTITUTE Ohio State University PR:LAST_NAME Cooperstone PR:FIRST_NAME Jessica PR:ADDRESS 2255 Kenny Rd PR:EMAIL cooperstone.1@osu.edu PR:PHONE 614-292-2843 #STUDY ST:STUDY_TITLE Tomato-Soy Juice Reduces Inflammation and Modulates Urinary Metabolome in Adults ST:STUDY_TITLE with Obesity ST:STUDY_SUMMARY Scope: Chronic, low-grade inflammation is a hallmark of many noncommunicable ST:STUDY_SUMMARY diseases, including obesity. Diets enriched with tomatoes and soy have been ST:STUDY_SUMMARY associated with better health outcomes in inflammation-related illnesses, with ST:STUDY_SUMMARY lycopene and isoflavones considered key bioactive components, respectively. On ST:STUDY_SUMMARY the basis that whole food combinations may exert greater effects than isolated ST:STUDY_SUMMARY phytochemicals, we examine the anti-inflammatory and metabolic effects of ST:STUDY_SUMMARY tomato-soy juice compared to a low carotenoid tomato juice control in obesity. ST:STUDY_SUMMARY Methods and results: In a randomized, crossover trial, 12 healthy adults with ST:STUDY_SUMMARY obesity were provided either tomato-soy juice (54 mg lycopene/d, 189.9 mg ST:STUDY_SUMMARY isoflavones/d) or a low carotenoid tomato juice (no isoflavones) daily for 4 ST:STUDY_SUMMARY weeks, then crossed over to the other treatment following a washout period. ST:STUDY_SUMMARY Plasma carotenoids, cytokines, and the urine metabolome were measured pre- and ST:STUDY_SUMMARY post-interventions. Plasma lycopene significantly increased by 2.48-fold after ST:STUDY_SUMMARY tomato-soy intake. IL-5, IL-12p70, and GM-CSF significantly decreased (P < ST:STUDY_SUMMARY 0.05), and TNF-alpha trended downward (P = 0.052) following tomato-soy. Soy ST:STUDY_SUMMARY isoflavones and their metabolites primarily distinguished post-tomato-soy urine ST:STUDY_SUMMARY profiles. Both interventions induced some shared metabolomic changes in the ST:STUDY_SUMMARY urine, indicating tomato-driven effects independent of lycopene. Conclusion: ST:STUDY_SUMMARY Tomato-soy intake reduced some pro-inflammatory cytokines and altered the urine ST:STUDY_SUMMARY metabolomic profile in adults with obesity, supporting future studies using this ST:STUDY_SUMMARY functional food product for other inflammation-related conditions. ST:INSTITUTE Ohio State University ST:LAST_NAME Cooperstone ST:FIRST_NAME Jessica ST:ADDRESS 2255 Kenny Rd ST:EMAIL cooperstone.1@osu.edu ST:PHONE 614-292-2843 ST:NUM_GROUPS 2 ST:TOTAL_SUBJECTS 12 ST:NUM_MALES 7 ST:NUM_FEMALES 5 #SUBJECT SU:SUBJECT_TYPE Human SU:SUBJECT_SPECIES Homo sapiens SU:TAXONOMY_ID 9606 SU:AGE_OR_AGE_RANGE 30-60 SU:GENDER Male and female SU:HUMAN_SMOKING_STATUS Non-smokers SU:HUMAN_INCLUSION_CRITERIA BMI 30-45, plasma total cholesterol levels at 250 mg/dL or lower, and SU:HUMAN_INCLUSION_CRITERIA triglyceride levels at 250 mg/dL or lower SU:HUMAN_EXCLUSION_CRITERIA Smoking, the use of daily prescription anti-inflammatory drugs, antibiotics SU:HUMAN_EXCLUSION_CRITERIA within 3 months prior to the study, use of SU:HUMAN_EXCLUSION_CRITERIA carotenoid/isoflavone/metabolism-altering supplements within 1 month of the SU:HUMAN_EXCLUSION_CRITERIA study, presence of autoimmune or metabolic disorders, presence of any SU:HUMAN_EXCLUSION_CRITERIA gastrointestinal or malabsorption conditions, indication of liver or kidney SU:HUMAN_EXCLUSION_CRITERIA disorders, and tomato or soy allergies. #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data SUBJECT_SAMPLE_FACTORS 6101-U1 6101_U1_C18NEG_59 Sample source:urine | Timepoint:pre Tomato Soy RAW_FILE_NAME=6101_U1_C18NEG_59.d SUBJECT_SAMPLE_FACTORS 6101-U1 6101_U1_C18POS_59 Sample source:urine | Timepoint:pre Tomato Soy RAW_FILE_NAME=6101_U1_C18POS_59.d SUBJECT_SAMPLE_FACTORS 6101-U2 6101_U2_C18NEG_30 Sample source:urine | Timepoint:post Tomato Soy RAW_FILE_NAME=6101_U2_C18NEG_30.d SUBJECT_SAMPLE_FACTORS 6101-U2 6101_U2_C18POS_30 Sample source:urine | Timepoint:post Tomato Soy RAW_FILE_NAME=6101_U2_C18POS_30.d SUBJECT_SAMPLE_FACTORS 6101-U3 6101_U3_C18POS_29_1 Sample source:urine | Timepoint:pre Control RAW_FILE_NAME=6101_U3_C18POS_29_1.d SUBJECT_SAMPLE_FACTORS 6101-U3 6101_U3_C18NEG_29 Sample source:urine | Timepoint:pre Control RAW_FILE_NAME=6101_U3_C18NEG_29.d SUBJECT_SAMPLE_FACTORS 6101-U4 6101_U4_C18POS_14 Sample source:urine | Timepoint:post Control RAW_FILE_NAME=6101_U4_C18POS_14.d SUBJECT_SAMPLE_FACTORS 6101-U4 6101_U4_C18NEG_14 Sample source:urine | Timepoint:post Control RAW_FILE_NAME=6101_U4_C18NEG_14.d SUBJECT_SAMPLE_FACTORS 6102-U1 6102_U1_C18POS_26_1 Sample source:urine | Timepoint:pre Tomato Soy RAW_FILE_NAME=6102_U1_C18POS_26_1.d SUBJECT_SAMPLE_FACTORS 6102-U1 6102_U1_C18NEG_26 Sample source:urine | Timepoint:pre Tomato Soy RAW_FILE_NAME=6102_U1_C18NEG_26.d SUBJECT_SAMPLE_FACTORS 6102-U2 6102_U2_C18POS_16 Sample source:urine | Timepoint:post Tomato Soy RAW_FILE_NAME=6102_U2_C18POS_16.d SUBJECT_SAMPLE_FACTORS 6102-U2 6102_U2_C18NEG_16 Sample source:urine | Timepoint:post Tomato Soy RAW_FILE_NAME=6102_U2_C18NEG_16.d SUBJECT_SAMPLE_FACTORS 6102-U3 6102_U3_C18POS_48 Sample source:urine | Timepoint:pre Control RAW_FILE_NAME=6102_U3_C18POS_48.d SUBJECT_SAMPLE_FACTORS 6102-U3 6102_U3_C18NEG_48 Sample source:urine | Timepoint:pre Control RAW_FILE_NAME=6102_U3_C18NEG_48.d SUBJECT_SAMPLE_FACTORS 6102-U4 6102_U4_C18POS_50 Sample source:urine | Timepoint:post Control RAW_FILE_NAME=6102_U4_C18POS_50.d SUBJECT_SAMPLE_FACTORS 6102-U4 6102_U4_C18NEG_50 Sample source:urine | Timepoint:post Control RAW_FILE_NAME=6102_U4_C18NEG_50.d SUBJECT_SAMPLE_FACTORS 6103-U1 6103_U1_C18POS_21 Sample source:urine | Timepoint:pre Tomato Soy RAW_FILE_NAME=6103_U1_C18POS_21.d SUBJECT_SAMPLE_FACTORS 6103-U1 6103_U1_C18NEG_21 Sample source:urine | Timepoint:pre Tomato Soy RAW_FILE_NAME=6103_U1_C18NEG_21.d SUBJECT_SAMPLE_FACTORS 6103-U2 6103_U2_C18POS_60 Sample source:urine | Timepoint:post Tomato Soy RAW_FILE_NAME=6103_U2_C18POS_60.d SUBJECT_SAMPLE_FACTORS 6103-U2 6103_U2_C18NEG_60 Sample source:urine | Timepoint:post Tomato Soy RAW_FILE_NAME=6103_U2_C18NEG_60.d SUBJECT_SAMPLE_FACTORS 6103-U3 6103_U3_C18POS_61 Sample source:urine | Timepoint:pre Control RAW_FILE_NAME=6103_U3_C18POS_61.d SUBJECT_SAMPLE_FACTORS 6103-U3 6103_U3_C18NEG_61 Sample source:urine | Timepoint:pre Control RAW_FILE_NAME=6103_U3_C18NEG_61.d SUBJECT_SAMPLE_FACTORS 6103-U4 6103_U4_C18POS_53 Sample source:urine | Timepoint:post Control RAW_FILE_NAME=6103_U4_C18POS_53.d SUBJECT_SAMPLE_FACTORS 6103-U4 6103_U4_C18NEG_53 Sample source:urine | Timepoint:post Control RAW_FILE_NAME=6103_U4_C18NEG_53.d SUBJECT_SAMPLE_FACTORS 6104-U1 6104_U1_C18POS_55 Sample source:urine | Timepoint:pre Control RAW_FILE_NAME=6104_U1_C18POS_55.d SUBJECT_SAMPLE_FACTORS 6104-U1 6104_U1_C18NEG_55 Sample source:urine | Timepoint:pre Control RAW_FILE_NAME=6104_U1_C18NEG_55.d SUBJECT_SAMPLE_FACTORS 6104-U2 6104_U2_C18POS_19 Sample source:urine | Timepoint:post Control RAW_FILE_NAME=6104_U2_C18POS_19.d SUBJECT_SAMPLE_FACTORS 6104-U2 6104_U2_C18NEG_19 Sample source:urine | Timepoint:post Control RAW_FILE_NAME=6104_U2_C18NEG_19.d SUBJECT_SAMPLE_FACTORS 6104-U3 6104_U3_C18POS_32 Sample source:urine | Timepoint:pre Tomato Soy RAW_FILE_NAME=6104_U3_C18POS_32.d SUBJECT_SAMPLE_FACTORS 6104-U3 6104_U3_C18NEG_32 Sample source:urine | Timepoint:pre Tomato Soy RAW_FILE_NAME=6104_U3_C18NEG_32.d SUBJECT_SAMPLE_FACTORS 6104-U4 6104_U4_C18POS_12 Sample source:urine | Timepoint:post Tomato Soy RAW_FILE_NAME=6104_U4_C18POS_12.d SUBJECT_SAMPLE_FACTORS 6104-U4 6104_U4_C18NEG_12 Sample source:urine | Timepoint:post Tomato Soy RAW_FILE_NAME=6104_U4_C18NEG_12.d SUBJECT_SAMPLE_FACTORS 6105-U1 6105_U1_C18POS_43 Sample source:urine | Timepoint:pre Tomato Soy RAW_FILE_NAME=6105_U1_C18POS_43.d SUBJECT_SAMPLE_FACTORS 6105-U1 6105_U1_C18NEG_43 Sample source:urine | Timepoint:pre Tomato Soy RAW_FILE_NAME=6105_U1_C18NEG_43.d SUBJECT_SAMPLE_FACTORS 6105-U2 6105_U2_C18POS_40 Sample source:urine | Timepoint:post Tomato Soy RAW_FILE_NAME=6105_U2_C18POS_40.d SUBJECT_SAMPLE_FACTORS 6105-U2 6105_U2_C18NEG_40 Sample source:urine | Timepoint:post Tomato Soy RAW_FILE_NAME=6105_U2_C18NEG_40.d SUBJECT_SAMPLE_FACTORS 6105-U3 6105_U3_C18POS_39 Sample source:urine | Timepoint:pre Control RAW_FILE_NAME=6105_U3_C18POS_39.d SUBJECT_SAMPLE_FACTORS 6105-U3 6105_U3_C18NEG_39 Sample source:urine | Timepoint:pre Control RAW_FILE_NAME=6105_U3_C18NEG_39.d SUBJECT_SAMPLE_FACTORS 6105-U4 6105_U4_C18POS_47 Sample source:urine | Timepoint:post Control RAW_FILE_NAME=6105_U4_C18POS_47.d SUBJECT_SAMPLE_FACTORS 6105-U4 6105_U4_C18NEG_47 Sample source:urine | Timepoint:post Control RAW_FILE_NAME=6105_U4_C18NEG_47.d SUBJECT_SAMPLE_FACTORS 6106-U1 6106_U1_C18POS_23 Sample source:urine | Timepoint:pre Control RAW_FILE_NAME=6106_U1_C18POS_23.d SUBJECT_SAMPLE_FACTORS 6106-U1 6106_U1_C18NEG_23 Sample source:urine | Timepoint:pre Control RAW_FILE_NAME=6106_U1_C18NEG_23.d SUBJECT_SAMPLE_FACTORS 6106-U2 6106_U2_C18POS_46 Sample source:urine | Timepoint:post Control RAW_FILE_NAME=6106_U2_C18POS_46.d SUBJECT_SAMPLE_FACTORS 6106-U2 6106_U2_C18NEG_46 Sample source:urine | Timepoint:post Control RAW_FILE_NAME=6106_U2_C18NEG_46.d SUBJECT_SAMPLE_FACTORS 6106-U3 6106_U3_C18POS_20 Sample source:urine | Timepoint:pre Tomato Soy RAW_FILE_NAME=6106_U3_C18POS_20.d SUBJECT_SAMPLE_FACTORS 6106-U3 6106_U3_C18NEG_20 Sample source:urine | Timepoint:pre Tomato Soy RAW_FILE_NAME=6106_U3_C18NEG_20.d SUBJECT_SAMPLE_FACTORS 6106-U4 6106_U4_C18POS_36 Sample source:urine | Timepoint:post Tomato Soy RAW_FILE_NAME=6106_U4_C18POS_36.d SUBJECT_SAMPLE_FACTORS 6106-U4 6106_U4_C18NEG_36 Sample source:urine | Timepoint:post Tomato Soy RAW_FILE_NAME=6106_U4_C18NEG_36.d SUBJECT_SAMPLE_FACTORS 6108-U1 6108_U1_C18POS_44 Sample source:urine | Timepoint:pre Tomato Soy RAW_FILE_NAME=6108_U1_C18POS_44.d SUBJECT_SAMPLE_FACTORS 6108-U1 6108_U1_C18NEG_44 Sample source:urine | Timepoint:pre Tomato Soy RAW_FILE_NAME=6108_U1_C18NEG_44.d SUBJECT_SAMPLE_FACTORS 6108-U2 6108_U2_C18POS_27_1 Sample source:urine | Timepoint:post Tomato Soy RAW_FILE_NAME=6108_U2_C18POS_27_1.d SUBJECT_SAMPLE_FACTORS 6108-U2 6108_U2_C18NEG_27 Sample source:urine | Timepoint:post Tomato Soy RAW_FILE_NAME=6108_U2_C18NEG_27.d SUBJECT_SAMPLE_FACTORS 6108-U3 6108_U3_C18POS_28_1 Sample source:urine | Timepoint:pre Control RAW_FILE_NAME=6108_U3_C18POS_28_1.d SUBJECT_SAMPLE_FACTORS 6108-U3 6108_U3_C18NEG_28 Sample source:urine | Timepoint:pre Control RAW_FILE_NAME=6108_U3_C18NEG_28.d SUBJECT_SAMPLE_FACTORS 6108-U4 6108_U4_C18POS_18 Sample source:urine | Timepoint:post Control RAW_FILE_NAME=6108_U4_C18POS_18.d SUBJECT_SAMPLE_FACTORS 6108-U4 6108_U4_C18NEG_18 Sample source:urine | Timepoint:post Control RAW_FILE_NAME=6108_U4_C18NEG_18.d SUBJECT_SAMPLE_FACTORS 6109-U1 6109_U1_C18POS_58 Sample source:urine | Timepoint:pre Control RAW_FILE_NAME=6109_U1_C18POS_58.d SUBJECT_SAMPLE_FACTORS 6109-U1 6109_U1_C18NEG_58 Sample source:urine | Timepoint:pre Control RAW_FILE_NAME=6109_U1_C18NEG_58.d SUBJECT_SAMPLE_FACTORS 6109-U2 6109_U2_C18POS_13 Sample source:urine | Timepoint:post Control RAW_FILE_NAME=6109_U2_C18POS_13.d SUBJECT_SAMPLE_FACTORS 6109-U2 6109_U2_C18NEG_13 Sample source:urine | 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6110_U2_C18NEG_34 Sample source:urine | Timepoint:post Control RAW_FILE_NAME=6110_U2_C18NEG_34.d SUBJECT_SAMPLE_FACTORS 6110-U3 6110_U3_C18POS_45 Sample source:urine | Timepoint:pre Tomato Soy RAW_FILE_NAME=6110_U3_C18POS_45.d SUBJECT_SAMPLE_FACTORS 6110-U3 6110_U3_C18NEG_45 Sample source:urine | Timepoint:pre Tomato Soy RAW_FILE_NAME=6110_U3_C18NEG_45.d SUBJECT_SAMPLE_FACTORS 6110-U4 6110_U4_C18POS_start-10 Sample source:urine | Timepoint:post Tomato Soy RAW_FILE_NAME=6110_U4_C18POS_start-10.d SUBJECT_SAMPLE_FACTORS 6110-U4 6110_U4_C18NEG_10 Sample source:urine | Timepoint:post Tomato Soy RAW_FILE_NAME=6110_U4_C18NEG_10.d SUBJECT_SAMPLE_FACTORS 6111-U1 6111_U1_C18POS_51 Sample source:urine | Timepoint:pre Control RAW_FILE_NAME=6111_U1_C18POS_51.d SUBJECT_SAMPLE_FACTORS 6111-U1 6111_U1_C18NEG_51 Sample source:urine | Timepoint:pre Control RAW_FILE_NAME=6111_U1_C18NEG_51.d SUBJECT_SAMPLE_FACTORS 6111-U2 6111_U2_C18POS_35 Sample source:urine | Timepoint:post Control 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6113_U2_C18POS_31 Sample source:urine | Timepoint:post Control RAW_FILE_NAME=6113_U2_C18POS_31.d SUBJECT_SAMPLE_FACTORS 6113-U2 6113_U2_C18NEG_31 Sample source:urine | Timepoint:post Control RAW_FILE_NAME=6113_U2_C18NEG_31.d SUBJECT_SAMPLE_FACTORS 6113-U3 6113_U3_C18POS_15 Sample source:urine | Timepoint:pre Tomato Soy RAW_FILE_NAME=6113_U3_C18POS_15.d SUBJECT_SAMPLE_FACTORS 6113-U3 6113_U3_C18NEG_15 Sample source:urine | Timepoint:pre Tomato Soy RAW_FILE_NAME=6113_U3_C18NEG_15.d SUBJECT_SAMPLE_FACTORS 6113-U4 6113_U4_C18POS_62 Sample source:urine | Timepoint:post Tomato Soy RAW_FILE_NAME=6113_U4_C18POS_62.d SUBJECT_SAMPLE_FACTORS 6113-U4 6113_U4_C18NEG_62 Sample source:urine | Timepoint:post Tomato Soy RAW_FILE_NAME=6113_U4_C18NEG_62.d SUBJECT_SAMPLE_FACTORS 6101-U1 6101_U1_HILICNEG_59 Sample source:urine | Timepoint:pre Tomato Soy RAW_FILE_NAME=6101_U1_HILICNEG_59.d SUBJECT_SAMPLE_FACTORS 6101-U1 6101_U1_HILICPOS_59 Sample source:urine | Timepoint:pre Tomato Soy RAW_FILE_NAME=6101_U1_HILICPOS_59.d SUBJECT_SAMPLE_FACTORS 6101-U2 6101_U2_HILICNEG_30 Sample source:urine | Timepoint:post Tomato Soy RAW_FILE_NAME=6101_U2_HILICNEG_30.d SUBJECT_SAMPLE_FACTORS 6101-U2 6101_U2_HILICPOS_30 Sample source:urine | Timepoint:post Tomato Soy RAW_FILE_NAME=6101_U2_HILICPOS_30.d SUBJECT_SAMPLE_FACTORS 6101-U3 6101_U3_HILICNEG_29 Sample source:urine | Timepoint:pre Control RAW_FILE_NAME=6101_U3_HILICNEG_29.d SUBJECT_SAMPLE_FACTORS 6101-U3 6101_U3_HILICPOS_29 Sample source:urine | Timepoint:pre Control RAW_FILE_NAME=6101_U3_HILICPOS_29.d SUBJECT_SAMPLE_FACTORS 6101-U4 6101_U4_HILICNEG_14 Sample source:urine | Timepoint:post Control RAW_FILE_NAME=6101_U4_HILICNEG_14.d SUBJECT_SAMPLE_FACTORS 6101-U4 6101_U4_HILICPOS_14 Sample source:urine | Timepoint:post Control RAW_FILE_NAME=6101_U4_HILICPOS_14.d SUBJECT_SAMPLE_FACTORS 6102-U1 6102-redo_U4_HILICNEG_50 Sample source:urine | Timepoint:pre Tomato Soy RAW_FILE_NAME=6102-redo_U4_HILICNEG_50.d SUBJECT_SAMPLE_FACTORS 6102-U1 6102_U1_HILICPOS_26 Sample source:urine | Timepoint:pre Tomato Soy RAW_FILE_NAME=6102_U1_HILICPOS_26.d SUBJECT_SAMPLE_FACTORS 6102-U2 6102_U1_HILICNEG_26 Sample source:urine | Timepoint:post Tomato Soy RAW_FILE_NAME=6102_U1_HILICNEG_26.d SUBJECT_SAMPLE_FACTORS 6102-U2 6102_U2_HILICPOS_16 Sample source:urine | Timepoint:post Tomato Soy RAW_FILE_NAME=6102_U2_HILICPOS_16.d SUBJECT_SAMPLE_FACTORS 6102-U3 6102_U2_HILICNEG_16 Sample source:urine | Timepoint:pre Control RAW_FILE_NAME=6102_U2_HILICNEG_16.d SUBJECT_SAMPLE_FACTORS 6102-U3 6102_U3_HILICPOS_48 Sample source:urine | Timepoint:pre Control RAW_FILE_NAME=6102_U3_HILICPOS_48.d SUBJECT_SAMPLE_FACTORS 6102-U4 6102_U3_HILICNEG_48 Sample source:urine | Timepoint:post Control RAW_FILE_NAME=6102_U3_HILICNEG_48.d SUBJECT_SAMPLE_FACTORS 6102-U4 6102_U4_HILICPOS_50 Sample source:urine | Timepoint:post Control RAW_FILE_NAME=6102_U4_HILICPOS_50.d SUBJECT_SAMPLE_FACTORS 6103-U1 6103-redo_U4_HILICNEG_53 Sample source:urine | Timepoint:pre Tomato Soy RAW_FILE_NAME=6103-redo_U4_HILICNEG_53.d SUBJECT_SAMPLE_FACTORS 6103-U1 6103_U1_HILICPOS_21 Sample source:urine | Timepoint:pre Tomato Soy RAW_FILE_NAME=6103_U1_HILICPOS_21.d SUBJECT_SAMPLE_FACTORS 6103-U2 6103_U1_HILICNEG_21 Sample source:urine | Timepoint:post Tomato Soy RAW_FILE_NAME=6103_U1_HILICNEG_21.d SUBJECT_SAMPLE_FACTORS 6103-U2 6103_U2_HILICPOS_60 Sample source:urine | Timepoint:post Tomato Soy RAW_FILE_NAME=6103_U2_HILICPOS_60.d SUBJECT_SAMPLE_FACTORS 6103-U3 6103_U2_HILICNEG_60 Sample source:urine | Timepoint:pre Control RAW_FILE_NAME=6103_U2_HILICNEG_60.d SUBJECT_SAMPLE_FACTORS 6103-U3 6103_U3_HILICPOS_61 Sample source:urine | Timepoint:pre Control RAW_FILE_NAME=6103_U3_HILICPOS_61.d SUBJECT_SAMPLE_FACTORS 6103-U4 6103_U3_HILICNEG_61 Sample source:urine | Timepoint:post Control RAW_FILE_NAME=6103_U3_HILICNEG_61.d SUBJECT_SAMPLE_FACTORS 6103-U4 6103_U4_HILICPOS_53 Sample source:urine | Timepoint:post Control RAW_FILE_NAME=6103_U4_HILICPOS_53.d SUBJECT_SAMPLE_FACTORS 6104-U1 6104-redo_U1_HILICNEG_55 Sample source:urine | Timepoint:pre Control RAW_FILE_NAME=6104-redo_U1_HILICNEG_55.d SUBJECT_SAMPLE_FACTORS 6104-U1 6104_U1_HILICPOS_55 Sample source:urine | Timepoint:pre Control RAW_FILE_NAME=6104_U1_HILICPOS_55.d SUBJECT_SAMPLE_FACTORS 6104-U2 6104_U2_HILICNEG_19 Sample source:urine | Timepoint:post Control RAW_FILE_NAME=6104_U2_HILICNEG_19.d SUBJECT_SAMPLE_FACTORS 6104-U2 6104_U2_HILICPOS_19 Sample source:urine | Timepoint:post Control RAW_FILE_NAME=6104_U2_HILICPOS_19.d SUBJECT_SAMPLE_FACTORS 6104-U3 6104_U3_HILICNEG_32 Sample source:urine | Timepoint:pre Tomato Soy RAW_FILE_NAME=6104_U3_HILICNEG_32.d SUBJECT_SAMPLE_FACTORS 6104-U3 6104_U3_HILICPOS_32 Sample source:urine | Timepoint:pre Tomato Soy RAW_FILE_NAME=6104_U3_HILICPOS_32.d SUBJECT_SAMPLE_FACTORS 6104-U4 6104_U4_HILICNEG_12 Sample source:urine | Timepoint:post Tomato Soy RAW_FILE_NAME=6104_U4_HILICNEG_12.d SUBJECT_SAMPLE_FACTORS 6104-U4 6104_U4_HILICPOS_12 Sample source:urine | Timepoint:post Tomato Soy RAW_FILE_NAME=6104_U4_HILICPOS_12.d SUBJECT_SAMPLE_FACTORS 6105-U1 6105_U1_HILICNEG_43 Sample source:urine | Timepoint:pre Tomato Soy RAW_FILE_NAME=6105_U1_HILICNEG_43.d SUBJECT_SAMPLE_FACTORS 6105-U1 6105_U1_HILICPOS_43 Sample source:urine | Timepoint:pre Tomato Soy RAW_FILE_NAME=6105_U1_HILICPOS_43.d SUBJECT_SAMPLE_FACTORS 6105-U2 6105_U2_HILICNEG_40 Sample source:urine | Timepoint:post Tomato Soy RAW_FILE_NAME=6105_U2_HILICNEG_40.d SUBJECT_SAMPLE_FACTORS 6105-U2 6105_U2_HILICPOS_40 Sample source:urine | Timepoint:post Tomato Soy RAW_FILE_NAME=6105_U2_HILICPOS_40.d SUBJECT_SAMPLE_FACTORS 6105-U3 6105_U3_HILICNEG_39 Sample source:urine | Timepoint:pre Control RAW_FILE_NAME=6105_U3_HILICNEG_39.d SUBJECT_SAMPLE_FACTORS 6105-U3 6105_U3_HILICPOS_39 Sample source:urine | Timepoint:pre Control RAW_FILE_NAME=6105_U3_HILICPOS_39.d SUBJECT_SAMPLE_FACTORS 6105-U4 6105_U4_HILICNEG_47 Sample source:urine | Timepoint:post Control RAW_FILE_NAME=6105_U4_HILICNEG_47.d SUBJECT_SAMPLE_FACTORS 6105-U4 6105_U4_HILICPOS_47 Sample source:urine | Timepoint:post Control RAW_FILE_NAME=6105_U4_HILICPOS_47.d SUBJECT_SAMPLE_FACTORS 6106-U1 6106_U1_HILICNEG_23 Sample source:urine | Timepoint:pre Control RAW_FILE_NAME=6106_U1_HILICNEG_23.d SUBJECT_SAMPLE_FACTORS 6106-U1 6106_U1_HILICPOS_23 Sample source:urine | Timepoint:pre Control RAW_FILE_NAME=6106_U1_HILICPOS_23.d SUBJECT_SAMPLE_FACTORS 6106-U2 6106_U2_HILICNEG_46 Sample source:urine | Timepoint:post Control RAW_FILE_NAME=6106_U2_HILICNEG_46.d SUBJECT_SAMPLE_FACTORS 6106-U2 6106_U2_HILICPOS_46 Sample source:urine | Timepoint:post Control RAW_FILE_NAME=6106_U2_HILICPOS_46.d SUBJECT_SAMPLE_FACTORS 6106-U3 6106_U3_HILICNEG_20 Sample source:urine | Timepoint:pre Tomato Soy RAW_FILE_NAME=6106_U3_HILICNEG_20.d SUBJECT_SAMPLE_FACTORS 6106-U3 6106_U3_HILICPOS_20 Sample source:urine | Timepoint:pre Tomato Soy RAW_FILE_NAME=6106_U3_HILICPOS_20.d SUBJECT_SAMPLE_FACTORS 6106-U4 6106_U4_HILICNEG_36 Sample source:urine | Timepoint:post Tomato Soy RAW_FILE_NAME=6106_U4_HILICNEG_36.d SUBJECT_SAMPLE_FACTORS 6106-U4 6106_U4_HILICPOS_36 Sample source:urine | Timepoint:post Tomato Soy RAW_FILE_NAME=6106_U4_HILICPOS_36.d SUBJECT_SAMPLE_FACTORS 6108-U1 6108_U1_HILICNEG_44 Sample source:urine | Timepoint:pre Tomato Soy RAW_FILE_NAME=6108_U1_HILICNEG_44.d SUBJECT_SAMPLE_FACTORS 6108-U1 6108_U1_HILICPOS_44 Sample source:urine | Timepoint:pre Tomato Soy RAW_FILE_NAME=6108_U1_HILICPOS_44.d SUBJECT_SAMPLE_FACTORS 6108-U2 6108_U2_HILICNEG_27 Sample source:urine | Timepoint:post Tomato Soy RAW_FILE_NAME=6108_U2_HILICNEG_27.d SUBJECT_SAMPLE_FACTORS 6108-U2 6108_U2_HILICPOS_27 Sample source:urine | Timepoint:post Tomato Soy RAW_FILE_NAME=6108_U2_HILICPOS_27.d SUBJECT_SAMPLE_FACTORS 6108-U3 6108_U3_HILICNEG_28 Sample source:urine | Timepoint:pre Control RAW_FILE_NAME=6108_U3_HILICNEG_28.d SUBJECT_SAMPLE_FACTORS 6108-U3 6108_U3_HILICPOS_28 Sample source:urine | Timepoint:pre Control RAW_FILE_NAME=6108_U3_HILICPOS_28.d SUBJECT_SAMPLE_FACTORS 6108-U4 6108_U4_HILICNEG_18 Sample source:urine | Timepoint:post Control RAW_FILE_NAME=6108_U4_HILICNEG_18.d SUBJECT_SAMPLE_FACTORS 6108-U4 6108_U4_HILICPOS_18 Sample source:urine | Timepoint:post Control RAW_FILE_NAME=6108_U4_HILICPOS_18.d SUBJECT_SAMPLE_FACTORS 6109-U1 6109-redo_U1_HILICNEG_58 Sample source:urine | Timepoint:pre Control RAW_FILE_NAME=6109-redo_U1_HILICNEG_58.d SUBJECT_SAMPLE_FACTORS 6109-U1 6109_U1_HILICPOS_58 Sample source:urine | Timepoint:pre Control RAW_FILE_NAME=6109_U1_HILICPOS_58.d SUBJECT_SAMPLE_FACTORS 6109-U2 6109_U2_HILICNEG_13 Sample source:urine | Timepoint:post Control RAW_FILE_NAME=6109_U2_HILICNEG_13.d SUBJECT_SAMPLE_FACTORS 6109-U2 6109_U2_HILICPOS_13 Sample source:urine | Timepoint:post Control RAW_FILE_NAME=6109_U2_HILICPOS_13.d SUBJECT_SAMPLE_FACTORS 6109-U3 6109-redo_U3_HILICNEG_52 Sample source:urine | Timepoint:pre Tomato Soy RAW_FILE_NAME=6109-redo_U3_HILICNEG_52.d SUBJECT_SAMPLE_FACTORS 6109-U3 6109_U3_HILICPOS_52 Sample source:urine | Timepoint:pre Tomato Soy RAW_FILE_NAME=6109_U3_HILICPOS_52.d SUBJECT_SAMPLE_FACTORS 6109-U4 6109_U4_HILICNEG_22 Sample source:urine | Timepoint:post Tomato Soy RAW_FILE_NAME=6109_U4_HILICNEG_22.d SUBJECT_SAMPLE_FACTORS 6109-U4 6109_U4_HILICPOS_22 Sample source:urine | Timepoint:post Tomato Soy RAW_FILE_NAME=6109_U4_HILICPOS_22.d SUBJECT_SAMPLE_FACTORS 6110-U1 6110_U1_HILICNEG_11 Sample source:urine | Timepoint:pre Control RAW_FILE_NAME=6110_U1_HILICNEG_11.d SUBJECT_SAMPLE_FACTORS 6110-U1 6110_U1_HILICPOS_11 Sample source:urine | Timepoint:pre Control RAW_FILE_NAME=6110_U1_HILICPOS_11.d SUBJECT_SAMPLE_FACTORS 6110-U2 6110_U2_HILICNEG_34 Sample source:urine | Timepoint:post Control RAW_FILE_NAME=6110_U2_HILICNEG_34.d SUBJECT_SAMPLE_FACTORS 6110-U2 6110_U2_HILICPOS_34 Sample source:urine | Timepoint:post Control RAW_FILE_NAME=6110_U2_HILICPOS_34.d SUBJECT_SAMPLE_FACTORS 6110-U3 6110_U3_HILICNEG_45 Sample source:urine | Timepoint:pre Tomato Soy RAW_FILE_NAME=6110_U3_HILICNEG_45.d SUBJECT_SAMPLE_FACTORS 6110-U3 6110_U3_HILICPOS_45 Sample source:urine | Timepoint:pre Tomato Soy RAW_FILE_NAME=6110_U3_HILICPOS_45.d SUBJECT_SAMPLE_FACTORS 6110-U4 6110_U4_HILICNEG_10 Sample source:urine | Timepoint:post Tomato Soy RAW_FILE_NAME=6110_U4_HILICNEG_10.d SUBJECT_SAMPLE_FACTORS 6110-U4 6110_U4_HILICPOS_10 Sample source:urine | Timepoint:post Tomato Soy RAW_FILE_NAME=6110_U4_HILICPOS_10.d SUBJECT_SAMPLE_FACTORS 6111-U1 6111-redo_U1_HILICNEG_51 Sample source:urine | Timepoint:pre Control RAW_FILE_NAME=6111-redo_U1_HILICNEG_51.d SUBJECT_SAMPLE_FACTORS 6111-U1 6111_U1_HILICPOS_51 Sample source:urine | Timepoint:pre Control RAW_FILE_NAME=6111_U1_HILICPOS_51.d SUBJECT_SAMPLE_FACTORS 6111-U2 6111_U2_HILICNEG_35 Sample source:urine | Timepoint:post Control RAW_FILE_NAME=6111_U2_HILICNEG_35.d SUBJECT_SAMPLE_FACTORS 6111-U2 6111_U2_HILICPOS_35 Sample source:urine | Timepoint:post Control RAW_FILE_NAME=6111_U2_HILICPOS_35.d SUBJECT_SAMPLE_FACTORS 6111-U3 6111-redo_U3_HILICNEG_54 Sample source:urine | Timepoint:pre Tomato Soy RAW_FILE_NAME=6111-redo_U3_HILICNEG_54.d SUBJECT_SAMPLE_FACTORS 6111-U3 6111_U3_HILICPOS_54 Sample source:urine | Timepoint:pre Tomato Soy RAW_FILE_NAME=6111_U3_HILICPOS_54.d SUBJECT_SAMPLE_FACTORS 6111-U4 6111_U4_HILICNEG_42 Sample source:urine | Timepoint:post Tomato Soy RAW_FILE_NAME=6111_U4_HILICNEG_42.d SUBJECT_SAMPLE_FACTORS 6111-U4 6111_U4_HILICPOS_42 Sample source:urine | Timepoint:post Tomato Soy RAW_FILE_NAME=6111_U4_HILICPOS_42.d SUBJECT_SAMPLE_FACTORS 6112-U1 6112_U1_HILICNEG_38 Sample source:urine | Timepoint:pre Tomato Soy RAW_FILE_NAME=6112_U1_HILICNEG_38.d SUBJECT_SAMPLE_FACTORS 6112-U1 6112_U1_HILICPOS_38 Sample source:urine | Timepoint:pre Tomato Soy RAW_FILE_NAME=6112_U1_HILICPOS_38.d SUBJECT_SAMPLE_FACTORS 6112-U2 6112_U2_HILICNEG_37 Sample source:urine | Timepoint:post Tomato Soy RAW_FILE_NAME=6112_U2_HILICNEG_37.d SUBJECT_SAMPLE_FACTORS 6112-U2 6112_U2_HILICPOS_37 Sample source:urine | Timepoint:post Tomato Soy RAW_FILE_NAME=6112_U2_HILICPOS_37.d SUBJECT_SAMPLE_FACTORS 6112-U3 6112-redo_U3_HILICNEG_56 Sample source:urine | Timepoint:pre Control RAW_FILE_NAME=6112-redo_U3_HILICNEG_56.d SUBJECT_SAMPLE_FACTORS 6112-U3 6112_U3_HILICPOS_56 Sample source:urine | Timepoint:pre Control RAW_FILE_NAME=6112_U3_HILICPOS_56.d SUBJECT_SAMPLE_FACTORS 6112-U4 6112_U4_HILICNEG_63 Sample source:urine | Timepoint:post Control RAW_FILE_NAME=6112_U4_HILICNEG_63.d SUBJECT_SAMPLE_FACTORS 6112-U4 6112_U4_HILICPOS_63 Sample source:urine | Timepoint:post Control RAW_FILE_NAME=6112_U4_HILICPOS_63.d SUBJECT_SAMPLE_FACTORS 6113-U1 6113_U1_HILICNEG_24 Sample source:urine | Timepoint:pre Control RAW_FILE_NAME=6113_U1_HILICNEG_24.d SUBJECT_SAMPLE_FACTORS 6113-U1 6113_U1_HILICPOS_24 Sample source:urine | Timepoint:pre Control RAW_FILE_NAME=6113_U1_HILICPOS_24.d SUBJECT_SAMPLE_FACTORS 6113-U2 6113_U2_HILICNEG_31 Sample source:urine | Timepoint:post Control RAW_FILE_NAME=6113_U2_HILICNEG_31.d SUBJECT_SAMPLE_FACTORS 6113-U2 6113_U2_HILICPOS_31 Sample source:urine | Timepoint:post Control RAW_FILE_NAME=6113_U2_HILICPOS_31.d SUBJECT_SAMPLE_FACTORS 6113-U3 6113_U3_HILICNEG_15 Sample source:urine | Timepoint:pre Tomato Soy RAW_FILE_NAME=6113_U3_HILICNEG_15.d SUBJECT_SAMPLE_FACTORS 6113-U3 6113_U3_HILICPOS_15 Sample source:urine | Timepoint:pre Tomato Soy RAW_FILE_NAME=6113_U3_HILICPOS_15.d SUBJECT_SAMPLE_FACTORS 6113-U4 6113_U4_HILICNEG_62 Sample source:urine | Timepoint:post Tomato Soy RAW_FILE_NAME=6113_U4_HILICNEG_62.d SUBJECT_SAMPLE_FACTORS 6113-U4 6113_U4_HILICPOS_62 Sample source:urine | Timepoint:post Tomato Soy RAW_FILE_NAME=6113_U4_HILICPOS_62.d #COLLECTION CO:COLLECTION_SUMMARY At weeks 2, 6, 10, and 14, 24-hour urine samples were collected from the CO:COLLECTION_SUMMARY subjects into 4 L plastic jugs containing 10 g boric acid for preservation. CO:COLLECTION_SUMMARY Urine samples were then sub-sampled into smaller aliquots and stored at -80 CO:COLLECTION_SUMMARY degrees C. CO:SAMPLE_TYPE Urine CO:STORAGE_CONDITIONS -80℃ #TREATMENT TR:TREATMENT_SUMMARY To test the effects of both lycopene and isoflavones on inflammation and the TR:TREATMENT_SUMMARY metabolome, subjects were instructed to consume diets with low levels of these TR:TREATMENT_SUMMARY phytochemicals for 2 weeks prior to the intervention. Counseling and a list of TR:TREATMENT_SUMMARY foods to avoid was provided to achieve a low lycopene/isoflavone diet. Following TR:TREATMENT_SUMMARY the 2-week washout period, subjects were randomized to receive 360 mL (two 180 TR:TREATMENT_SUMMARY mL cans) of either a soy-enriched, high lycopene tomato (tomato-soy) juice or a TR:TREATMENT_SUMMARY low carotenoid, yellow tomato juice (control) every day for 4 weeks. Followed by TR:TREATMENT_SUMMARY a 4-week washout period (low lycopene and isoflavone diet), participants began TR:TREATMENT_SUMMARY their second 4-week intervention consuming the other juice daily (Figure 2). At TR:TREATMENT_SUMMARY weeks 2, 6, 10, and 14, 24-hour urine samples were collected from the subjects TR:TREATMENT_SUMMARY into 4 L plastic jugs containing 10 g boric acid for preservation. Urine samples TR:TREATMENT_SUMMARY were then sub-sampled into smaller aliquots and stored at -80 degrees C. This TR:TREATMENT_SUMMARY study was approved by Chesapeake IRB (Pro00024511) and is registered on TR:TREATMENT_SUMMARY clinicaltrials.gov as NCT03783013. #SAMPLEPREP SP:SAMPLEPREP_SUMMARY After thawing in cold water, urine was centrifuged for 10 min at 21,130 x g. SP:SAMPLEPREP_SUMMARY Urine osmolality was measured by freezing point depression using an osmometer SP:SAMPLEPREP_SUMMARY (Advanced Osmometer Model 3300; Norwood, MA, USA). To account for differences in SP:SAMPLEPREP_SUMMARY urine volumes and hydration level between subjects/timepoints, each sample was SP:SAMPLEPREP_SUMMARY normalized to 100 milliosmoles with water. Normalized urine samples were added SP:SAMPLEPREP_SUMMARY to new sample tubes containing methanol and acetonitrile, so that the final SP:SAMPLEPREP_SUMMARY mixture was the injection solvent 2:1:1 ACN/MeOH/H2O and centrifuged at 21,130 x SP:SAMPLEPREP_SUMMARY g for 5 min to pellet any insoluble material. Process blanks were prepared using SP:SAMPLEPREP_SUMMARY water following the same steps. Quality control (QC) samples were prepared by SP:SAMPLEPREP_SUMMARY pooling 100 microliters from each normalized urine sample. SP:PROCESSING_STORAGE_CONDITIONS Room temperature SP:EXTRACT_STORAGE -80℃ #CHROMATOGRAPHY CH:CHROMATOGRAPHY_SUMMARY For each analysis, sample order was randomized, with QC samples in every 7th CH:CHROMATOGRAPHY_SUMMARY position (i.e., approximately every 1.5-2 h) to monitor instrument variability CH:CHROMATOGRAPHY_SUMMARY throughout the duration of the experimental run. Three process blanks were CH:CHROMATOGRAPHY_SUMMARY queued at the beginning of each run to account for residues and contamination CH:CHROMATOGRAPHY_SUMMARY from extraction materials and remove them from downstream analyses. HILIC CH:CHROMATOGRAPHY_SUMMARY analysis was adapted from methods in Spagou et al (2011). Twenty uL was injected CH:CHROMATOGRAPHY_SUMMARY onto a 2.1 x 100 mm x 1.8 micron Waters Z-HILIC column maintained at 40 degrees CH:CHROMATOGRAPHY_SUMMARY C. CH:CHROMATOGRAPHY_TYPE HILIC CH:INSTRUMENT_NAME Agilent 1290 Infinity CH:COLUMN_NAME Waters Z-HILIC (100 x 2.1mm; 1.8um) CH:SOLVENT_A 50% acetonitrile/50% water; 10 mM ammonium formate (pH 7.2); 0.1% formic acid CH:SOLVENT_B 95% acetonitrile/5% water; 10 mM ammonium formate (pH 7.2); 0.1% formic acid CH:FLOW_GRADIENT The 14 min gradient was applied at 0.5 mL/min as described: held at 100% B for 1 CH:FLOW_GRADIENT min, increased linearly to 100% A over 10 min and held at this point for 1 min. CH:FLOW_GRADIENT Immediately afterwards, the gradient was held at 100% B for 2 min CH:FLOW_RATE 0.5 mL/min CH:COLUMN_TEMPERATURE 40 #ANALYSIS AN:ANALYSIS_TYPE MS #MS MS:INSTRUMENT_NAME Agilent 6546 QTOF MS:INSTRUMENT_TYPE QTOF MS:MS_TYPE ESI MS:ION_MODE NEGATIVE MS:MS_COMMENTS Full scan data was acquired in a mass range of 50-1700 m/z with a scan rate of 3 MS:MS_COMMENTS spectra/sec. MS parameters were set as followed: gas temperature of 350 degrees MS:MS_COMMENTS C, gas flow at 10 L/min, nebulizer at 35 psig, sheath gas temperature at 375 MS:MS_COMMENTS degrees C, and sheath gas flow at 12 L/min. MS:MS_RESULTS_FILE ST004178_AN006936_Results.txt UNITS:Peak area Has m/z:Yes Has RT:Yes RT units:Minutes #END