#METABOLOMICS WORKBENCH Jung_DNLI_20250905_111043 DATATRACK_ID:6386 STUDY_ID:ST004205 ANALYSIS_ID:AN006992 PROJECT_ID:PR002651 VERSION 1 CREATED_ON September 19, 2025, 6:13 pm #PROJECT PR:PROJECT_TITLE GPR34 regulates microglia state and loss-of-function rescues TREM2 metabolic PR:PROJECT_TITLE dysfunction PR:PROJECT_TYPE cellular study PR:PROJECT_SUMMARY Microglia are implicated in modifying neurodegenerative disease risk in the PR:PROJECT_SUMMARY central nervous system (CNS). GPR34 is a microglia-enriched G-protein coupled PR:PROJECT_SUMMARY receptor that detects cytotoxic lipids upregulated in Alzheimer’s Disease PR:PROJECT_SUMMARY (AD). Since dysregulated lipid metabolism occurs in disease, we hypothesized PR:PROJECT_SUMMARY GPR34 could act with other lipid sensors, such as TREM2, to regulate microglial PR:PROJECT_SUMMARY function. Here, we report that GPR34 knockout (KO) rescues dysregulated PR:PROJECT_SUMMARY cholesterol metabolism in TREM2 KO iPSC-derived microglia (iMG) and alone PR:PROJECT_SUMMARY promotes fatty acid catabolism without the proton leak observed in TREM2 KO. PR:PROJECT_SUMMARY Loss of GPR34 downregulated ERK signaling, while its agonism promoted PR:PROJECT_SUMMARY interaction with and activation of ERK. In healthy and amyloid mouse models, PR:PROJECT_SUMMARY Gpr34 KO accelerated the conversion of homeostatic microglia to PR:PROJECT_SUMMARY disease-associated microglia (DAM) states. Additionally, in Gpr34 KO amyloid PR:PROJECT_SUMMARY mouse brain, the frequency of large plaques was increased compared to WT, PR:PROJECT_SUMMARY indicating that Gpr34 KO microglia may promote amyloid aggregation. Overall, our PR:PROJECT_SUMMARY data suggest GPR34 as a therapeutic target for modulating microglial function to PR:PROJECT_SUMMARY slow AD progression. PR:INSTITUTE Denali Therapeutics PR:LAST_NAME Suh PR:FIRST_NAME Jung PR:ADDRESS 161 Oyster Point Blvd, South San Francisco, California, 94080, USA PR:EMAIL suh@dnli.com PR:PHONE +1 6507973837 #STUDY ST:STUDY_TITLE Lipidomic/Metabolomic Characterization of GPR34 KO, WT, TREM2 KO, and ST:STUDY_TITLE GPR34/TREM2 dKO iPSC1-derived microglia ST:STUDY_SUMMARY GPR34 is a microglia-enriched receptor that senses cytotoxic lipids in ST:STUDY_SUMMARY Alzheimer’s disease and may interact with other lipid sensors like TREM2 to ST:STUDY_SUMMARY regulate metabolism. Using targeted lipid and metabolite profiling of ST:STUDY_SUMMARY iPSC-derived microglia with GPR34 KO, TREM2 KO, or GPR34/TREM2 double KO, we ST:STUDY_SUMMARY found that GPR34 deletion restores cholesterol metabolism defects in TREM2 KO ST:STUDY_SUMMARY cells and independently enhances fatty acid catabolism. ST:INSTITUTE Denali Therapeutics ST:LAST_NAME Suh ST:FIRST_NAME Jung ST:ADDRESS 161 Oyster Point Blvd ST:EMAIL suh@dnli.com ST:PHONE +1 6507973837 #SUBJECT SU:SUBJECT_TYPE Human SU:SUBJECT_SPECIES Homo sapiens SU:TAXONOMY_ID 9606 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data SUBJECT_SAMPLE_FACTORS Pool_1 HSA-000028400 Genotype:NA | Treatment:NA | Sample source:iMicroglia RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_001; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_001; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_001 SUBJECT_SAMPLE_FACTORS WT3 Myelin HSA-000028164 Genotype:WT | Treatment:Myelin | Sample source:iMicroglia RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_002; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_002; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_002 SUBJECT_SAMPLE_FACTORS GPR34KO4 Myelin HSA-000028179 Genotype:GPR34-KO | Treatment:Myelin | Sample source:iMicroglia RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_004; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_004; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_004 SUBJECT_SAMPLE_FACTORS dKO A4 4 Myelin HSA-000028185 Genotype:GPR34/TREM2-KO | Treatment:Myelin | Sample source:iMicroglia RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_005; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_005; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_005 SUBJECT_SAMPLE_FACTORS dKO A4 3 Myelin HSA-000028173 Genotype:GPR34/TREM2-KO | Treatment:Myelin | Sample source:iMicroglia RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_006; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_006; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_006 SUBJECT_SAMPLE_FACTORS TREM2KO1 Myelin HSA-000028146 Genotype:TREM2-KO | Treatment:Myelin | Sample source:iMicroglia RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_007; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_007; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_007 SUBJECT_SAMPLE_FACTORS GPR34KO1 Myelin HSA-000028143 Genotype:GPR34-KO | Treatment:Myelin | Sample source:iMicroglia RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_009; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_009; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_009 SUBJECT_SAMPLE_FACTORS dKO A4 4 PBS HSA-000028184 Genotype:GPR34/TREM2-KO | Treatment:PBS | Sample source:iMicroglia RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_010; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_010; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_010 SUBJECT_SAMPLE_FACTORS TREM2KO3 PBS HSA-000028169 Genotype:TREM2-KO | Treatment:PBS | Sample source:iMicroglia RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_011; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_011; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_011 SUBJECT_SAMPLE_FACTORS GPR34KO3 Myelin HSA-000028167 Genotype:GPR34-KO | Treatment:Myelin | Sample source:iMicroglia RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_012; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_012; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_012 SUBJECT_SAMPLE_FACTORS TREM2KO2 PBS HSA-000028157 Genotype:TREM2-KO | Treatment:PBS | Sample source:iMicroglia RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_013; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_013; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_013 SUBJECT_SAMPLE_FACTORS TREM2KO4 Myelin HSA-000028182 Genotype:TREM2-KO | Treatment:Myelin | Sample source:iMicroglia RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_016; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_016; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_016 SUBJECT_SAMPLE_FACTORS Pool_2 HSA-000028401 Genotype:NA | Treatment:NA | Sample source:iMicroglia RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_017; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_017; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_017 SUBJECT_SAMPLE_FACTORS WT3 PBS HSA-000028163 Genotype:WT | Treatment:PBS | Sample source:iMicroglia RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_019; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_019; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_019 SUBJECT_SAMPLE_FACTORS TREM2KO2 Myelin HSA-000028158 Genotype:TREM2-KO | Treatment:Myelin | Sample source:iMicroglia RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_020; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_020; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_020 SUBJECT_SAMPLE_FACTORS dKO A4 2 Myelin HSA-000028161 Genotype:GPR34/TREM2-KO | Treatment:Myelin | Sample source:iMicroglia RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_021; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_021; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_021 SUBJECT_SAMPLE_FACTORS GPR34KO3 PBS HSA-000028166 Genotype:GPR34 KO | Treatment:PBS | Sample source:iMicroglia RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_022; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_022; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_022 SUBJECT_SAMPLE_FACTORS WT2 PBS HSA-000028151 Genotype:WT | Treatment:PBS | Sample source:iMicroglia RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_023; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_023; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_023 SUBJECT_SAMPLE_FACTORS GPR34KO4 PBS HSA-000028178 Genotype:GPR34-KO | Treatment:PBS | Sample source:iMicroglia RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_024; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_024; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_024 SUBJECT_SAMPLE_FACTORS TREM2KO3 Myelin HSA-000028170 Genotype:TREM2-KO | Treatment:Myelin | Sample source:iMicroglia RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_026; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_026; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_026 SUBJECT_SAMPLE_FACTORS WT2 Myelin HSA-000028152 Genotype:WT | Treatment:Myelin | Sample source:iMicroglia RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_027; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_027; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_027 SUBJECT_SAMPLE_FACTORS GPR34KO1 PBS HSA-000028142 Genotype:GPR34-KO | Treatment:PBS | Sample source:iMicroglia RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_028; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_028; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_028 SUBJECT_SAMPLE_FACTORS WT1 Myelin HSA-000028140 Genotype:WT | Treatment:Myelin | Sample source:iMicroglia RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_031; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_031; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_031 SUBJECT_SAMPLE_FACTORS dKO A4 1 Myelin HSA-000028149 Genotype:GPR34/TREM2-KO | Treatment:Myelin | Sample source:iMicroglia RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_032; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_032; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_032 SUBJECT_SAMPLE_FACTORS Pool_3 HSA-000028402 Genotype:NA | Treatment:NA | Sample source:iMicroglia RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_035; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_035; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_035 SUBJECT_SAMPLE_FACTORS dKO A4 1 PBS HSA-000028148 Genotype:GPR34/TREM2-KO | Treatment:PBS | Sample source:iMicroglia RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_036; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_036; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_036 SUBJECT_SAMPLE_FACTORS dKO A4 3 PBS HSA-000028172 Genotype:GPR34/TREM2-KO | Treatment:PBS | Sample source:iMicroglia RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_038; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_038; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_038 SUBJECT_SAMPLE_FACTORS dKO A4 2 PBS HSA-000028160 Genotype:GPR34/TREM2-KO | Treatment:PBS | Sample source:iMicroglia RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_041; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_041; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_041 SUBJECT_SAMPLE_FACTORS TREM2KO1 PBS HSA-000028145 Genotype:TREM2-KO | Treatment:PBS | Sample source:iMicroglia RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_043; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_043; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_043 SUBJECT_SAMPLE_FACTORS WT4 Myelin HSA-000028176 Genotype:WT | Treatment:Myelin | Sample source:iMicroglia RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_045; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_045; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_045 SUBJECT_SAMPLE_FACTORS WT1 PBS HSA-000028139 Genotype:WT | Treatment:PBS | Sample source:iMicroglia RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_046; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_046; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_046 SUBJECT_SAMPLE_FACTORS WT4 PBS HSA-000028175 Genotype:WT | Treatment:PBS | Sample source:iMicroglia RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_047; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_047; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_047 SUBJECT_SAMPLE_FACTORS GPR34KO2 PBS HSA-000028154 Genotype:GPR34-KO | Treatment:PBS | Sample source:iMicroglia RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_049; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_049; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_049 SUBJECT_SAMPLE_FACTORS TREM2KO4 PBS HSA-000028181 Genotype:TREM2-KO | Treatment:PBS | Sample source:iMicroglia RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_050; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_050; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_050 SUBJECT_SAMPLE_FACTORS GPR34KO2 Myelin HSA-000028155 Genotype:GPR34-KO | Treatment:Myelin | Sample source:iMicroglia RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_051; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_051; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_051 SUBJECT_SAMPLE_FACTORS Pool_4 HSA-000028403 Genotype:NA | Treatment:NA | Sample source:iMicroglia RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_052; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_052; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_052 #COLLECTION CO:COLLECTION_SUMMARY iPSC1-derived microglia were harvested by aspirating medium then briefly washed CO:COLLECTION_SUMMARY with 1mL of ice-cold PBS solution and directly processed for lipidomic and CO:COLLECTION_SUMMARY metabolomic analysis. CO:SAMPLE_TYPE IPSC1-derived microglia CO:STORAGE_CONDITIONS -80℃ CO:COLLECTION_VIALS Lobind 1.5 mL Eppendorf tubes CO:STORAGE_VIALS Lobind 1.5 mL Eppendorf tubes #TREATMENT TR:TREATMENT_SUMMARY Demyelination is a key feature of neuronal degeneration in vivo in AD and TR:TREATMENT_SUMMARY amyloid mouse models (see Nugent et al. 2020, TR:TREATMENT_SUMMARY https://doi.org/10.1016/j.neuron.2019.12.007). For this reason, we selected it TR:TREATMENT_SUMMARY as a stimulus to evaluate microglial metabolism under a physiologically relevant TR:TREATMENT_SUMMARY stressor in vitro. Cells were treated for 48 hours with PBS or Myelin, followed TR:TREATMENT_SUMMARY by lipid extraction. #SAMPLEPREP SP:SAMPLEPREP_SUMMARY Cells were harvested by aspirating the medium, briefly washed with 1 mL of SP:SAMPLEPREP_SUMMARY ice-cold PBS, and then lysed in 50 µL methanol containing stable-isotope SP:SAMPLEPREP_SUMMARY internal standards. Samples were vortexed, adjusted to 100 µl with MS-grade SP:SAMPLEPREP_SUMMARY H₂O, and transferred to 1.5 ml Protein LoBind tubes on ice. 100 µl of Methyl SP:SAMPLEPREP_SUMMARY tert-butyl ether (MTBE)was added, vortexed and centrifuged at 21,000 g for 10 SP:SAMPLEPREP_SUMMARY min at 4 °C. The two phases were separated: the top (non-polar lipids) and SP:SAMPLEPREP_SUMMARY bottom (polar metabolites) were collected into glass vials, dried overnight in a SP:SAMPLEPREP_SUMMARY Genevac EZ3, and resuspended in 100 µl of methanol (lipids) or 9:1 SP:SAMPLEPREP_SUMMARY methanol:H₂O (metabolites) for LC-MS/MS analysis. SP:PROCESSING_STORAGE_CONDITIONS On ice SP:EXTRACT_STORAGE -20℃ #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE Reversed phase CH:INSTRUMENT_NAME Agilent 1290 Infinity II CH:COLUMN_NAME Waters ACQUITY UPLC BEH C18 (100 x 2.1mm,1.7um) CH:SOLVENT_A 60% acetonitrile/40% water; 10 mM ammonium formate; 0.1% formic acid CH:SOLVENT_B 90% isopropyl alcohol/10% acetonitrile; 10 mM ammonium formate; 0.1% formic acid CH:FLOW_GRADIENT 0.0-8.0 min from 45% B to 99% B, 8.0-9.0 min at 99% B, 9.0-9.1 min to 45% B, and CH:FLOW_GRADIENT 9.1-10.0 min at 45% B. CH:FLOW_RATE 0.25 mL/min CH:COLUMN_TEMPERATURE 55 CH:SAMPLE_INJECTION 5 #ANALYSIS AN:ANALYSIS_TYPE MS #MS MS:INSTRUMENT_NAME ABI Sciex 6500+ QTrap MS:INSTRUMENT_TYPE Triple quadrupole MS:MS_TYPE ESI MS:ION_MODE POSITIVE MS:MS_COMMENTS curtain gas at 40 psi; collision gas set at medium; ion spray voltage at 5,500 MS:MS_COMMENTS V; temperature at 250°C; ion source Gas 1 at 55 psi; ion source Gas 2 at 60 MS:MS_COMMENTS psi. #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS normalized peak area MS_METABOLITE_DATA_START Samples HSA-000028139 HSA-000028140 HSA-000028142 HSA-000028143 HSA-000028145 HSA-000028146 HSA-000028148 HSA-000028149 HSA-000028151 HSA-000028152 HSA-000028154 HSA-000028155 HSA-000028157 HSA-000028158 HSA-000028160 HSA-000028161 HSA-000028163 HSA-000028164 HSA-000028166 HSA-000028167 HSA-000028169 HSA-000028170 HSA-000028172 HSA-000028173 HSA-000028175 HSA-000028176 HSA-000028178 HSA-000028179 HSA-000028181 HSA-000028182 HSA-000028184 HSA-000028185 HSA-000028400 HSA-000028401 HSA-000028402 HSA-000028403 Factors Genotype:WT | Treatment:PBS | Sample source:iMicroglia Genotype:WT | Treatment:Myelin | Sample source:iMicroglia Genotype:GPR34-KO | Treatment:PBS | Sample source:iMicroglia Genotype:GPR34-KO | Treatment:Myelin | Sample source:iMicroglia Genotype:TREM2-KO | Treatment:PBS | Sample source:iMicroglia Genotype:TREM2-KO | Treatment:Myelin | Sample source:iMicroglia Genotype:GPR34/TREM2-KO | Treatment:PBS | Sample source:iMicroglia Genotype:GPR34/TREM2-KO | Treatment:Myelin | Sample source:iMicroglia Genotype:WT | Treatment:PBS | Sample source:iMicroglia Genotype:WT | Treatment:Myelin | Sample source:iMicroglia Genotype:GPR34-KO | Treatment:PBS | Sample source:iMicroglia Genotype:GPR34-KO | Treatment:Myelin | Sample source:iMicroglia Genotype:TREM2-KO | Treatment:PBS | Sample source:iMicroglia Genotype:TREM2-KO | Treatment:Myelin | Sample source:iMicroglia Genotype:GPR34/TREM2-KO | Treatment:PBS | Sample source:iMicroglia Genotype:GPR34/TREM2-KO | Treatment:Myelin | Sample source:iMicroglia Genotype:WT | Treatment:PBS | Sample source:iMicroglia Genotype:WT | Treatment:Myelin | Sample source:iMicroglia Genotype:GPR34 KO | Treatment:PBS | Sample source:iMicroglia Genotype:GPR34-KO | Treatment:Myelin | Sample source:iMicroglia Genotype:TREM2-KO | Treatment:PBS | Sample source:iMicroglia Genotype:TREM2-KO | Treatment:Myelin | Sample source:iMicroglia Genotype:GPR34/TREM2-KO | Treatment:PBS | Sample source:iMicroglia Genotype:GPR34/TREM2-KO | Treatment:Myelin | Sample source:iMicroglia Genotype:WT | Treatment:PBS | Sample source:iMicroglia Genotype:WT | Treatment:Myelin | Sample source:iMicroglia Genotype:GPR34-KO | Treatment:PBS | Sample source:iMicroglia Genotype:GPR34-KO | Treatment:Myelin | Sample source:iMicroglia Genotype:TREM2-KO | Treatment:PBS | Sample source:iMicroglia Genotype:TREM2-KO | Treatment:Myelin | Sample source:iMicroglia Genotype:GPR34/TREM2-KO | Treatment:PBS | Sample source:iMicroglia Genotype:GPR34/TREM2-KO | Treatment:Myelin | Sample source:iMicroglia Genotype:NA | Treatment:NA | Sample source:iMicroglia Genotype:NA | Treatment:NA | Sample source:iMicroglia Genotype:NA | Treatment:NA | Sample source:iMicroglia Genotype:NA | Treatment:NA | Sample source:iMicroglia 1-O-Palmitoyl-Cer(d18:1/18:0) N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. 24-Hydroxycholesterol N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. 24-Hydroxycholesterol(d7) N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. 3-O-SulfoLacCer(d18:1/18:0) N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. 4-beta-Hydroxycholesterol N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. 7-keto-Cholesterol N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. CE HETE N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. CE HODE N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. CE HpODE N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. CE oxoHETE N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. CE oxoODE N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. CE(16:1) 0.018 0.186 0.016 0.102 0.009 0.185 0.014 0.062 0.02 0.276 0.021 0.111 0.024 0.283 0.017 0.032 0.013 0.254 0.02 0.069 0.015 0.155 0.016 0.038 0.016 0.187 0.042 0.07 0.017 0.175 0.018 0.059 0.08 0.101 0.072 0.063 CE(18:1(d7)) N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. CE(18:1) 0.187 3.119 0.184 1.343 0.153 2.72 0.16 0.663 0.196 4.74 0.188 1.724 0.195 5.175 0.171 0.378 0.184 5.063 0.187 1.195 0.184 2.665 0.173 0.563 0.206 3.751 0.272 1.165 0.183 3.588 0.188 0.747 1.108 1.144 0.95 0.926 CE(18:2) 0.134 1.162 0.13 0.759 0.067 1.241 0.097 0.441 0.168 1.766 0.18 0.9 0.173 1.98 0.109 0.245 0.134 1.567 0.172 0.596 0.146 1.274 0.118 0.349 0.166 1.361 0.421 0.612 0.13 1.492 0.152 0.47 0.553 0.683 0.478 0.424 CE(20:4) 0.443 5.871 0.567 7 0.235 12.124 0.254 3.721 0.368 7.398 0.453 7.703 0.433 18.849 0.25 2.314 0.338 8.133 0.501 6.37 0.484 14.2 0.293 3.576 0.542 9.716 0.97 6.247 0.416 14.969 0.34 4.378 3.548 4.091 2.873 2.759 CE(20:5) 0.16 3.731 0.219 5.05 0.069 7.974 0.091 2.965 0.07 4.857 0.126 8.003 0.1 15.452 0.036 0.788 0.074 4.886 0.189 7.195 0.14 10.502 0.08 2.714 0.187 5.575 0.217 6.469 0.145 15.23 0.107 4.263 2.139 2.198 1.765 1.687 CE(22:6) 0.189 10.023 0.173 6.738 0.061 10.883 0.097 3.402 0.139 14.449 0.178 10.972 0.128 21.077 0.067 0.741 0.144 14.2 0.227 7.979 0.142 10.452 0.095 2.477 0.256 10.911 0.311 8.212 0.133 16.609 0.117 3.85 3.421 3.683 2.886 2.643 Cer(d18:0/16:0) N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. Cer(d18:0/18:0) N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. Cer(d18:0/24:0) N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. Cer(d18:0/24:1) N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. Cer(d18:1/16:0(d7)) N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. Cer(d18:1/16:0) 3.772 5.487 4.277 6.328 2.631 6.124 4.431 7.085 4.032 6.037 5.587 8.051 3.072 8.719 1.37 3.031 3.922 6.247 6.542 9.428 4.139 8.038 3.612 6.056 4.119 6.564 5.49 7.111 4.316 9.343 3.931 7.705 5.427 4.965 5.126 4.682 Cer(d18:1/18:0) 0.751 5.53 1.006 4.952 0.825 4.932 1.428 5.622 0.693 7.067 1.777 6.616 1.111 5.9 0.346 4.255 0.738 7.121 2.477 7.198 1.434 6.545 1.275 5.736 0.871 6.522 1.947 7.291 1.648 7.917 1.58 7.602 3.061 2.85 2.928 2.861 Cer(d18:1/24:0) 4.467 9.937 6.352 11.18 3.925 11.066 6.828 13.291 5.271 12.876 13.668 20.874 4.958 18.131 1.851 4.971 4.508 11.403 19.527 25.94 6.672 16.179 6.032 12.317 5.045 10.141 11.971 19.646 9.216 23.212 8.849 18.773 10.287 9.277 9.714 9.508 Cer(d18:1/24:1) 5.916 25.524 8.364 25.592 5.906 24.266 8.752 28.656 6.218 31.296 11.009 33.621 4.736 31.527 2.725 17.844 6.361 29.586 13.412 34.589 6.629 31.62 7.245 28.025 6.844 28.788 10.691 31.858 7.657 37.703 8.001 34.814 17.277 16.279 16.289 16.16 Cholesterol 2.175 3.843 1.721 4.345 0.965 3.612 2.097 4.926 2.644 4.33 3.559 5.168 1.563 3.464 0.936 5.174 2.167 4.327 4.499 6.063 1.916 4.541 2.16 5.08 1.772 4.974 2.752 5.975 2.183 4.812 2.43 7.178 3.571 2.964 3.092 3.061 Cholesterol(d7) N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. Cholesteryl hexoside 0.003 0.015 0.003 0.012 0.003 0.018 0.004 0.014 0.004 0.025 0.005 0.019 0.004 0.04 0.002 0.007 0.004 0.028 0.007 0.024 0.005 0.026 0.004 0.014 0.004 0.017 0.005 0.019 0.006 0.039 0.005 0.02 0.009 0.012 0.009 0.008 Coenzyme Q10 0.004 0.005 0.003 0.004 0.002 0.003 0.006 0.006 0.005 0.007 0.009 0.012 0.004 0.005 0.002 0.002 0.004 0.005 0.013 0.015 0.006 0.006 0.007 0.007 0.005 0.006 0.009 0.007 0.01 0.008 0.008 0.009 0.006 0.006 0.006 0.006 DG(15:0/18:1(d7)) N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. DG(16:0_18:1) 0.577 1.034 0.547 1.411 0.224 0.922 0.488 1.542 0.652 1.771 0.894 1.669 0.396 0.747 0.271 1.747 0.775 0.992 1.129 1.53 0.524 1.385 0.53 1.461 1.107 1.898 0.7 1.261 0.6 1.235 0.379 1.418 0.994 0.965 0.993 0.97 DG(16:0_20:4) 0.081 0.232 0.159 0.509 0.031 0.347 0.127 0.59 0.064 0.209 0.199 0.374 0.078 0.181 0.03 0.845 0.09 0.186 0.27 0.38 0.177 0.469 0.139 0.598 0.228 0.624 0.243 0.512 0.189 0.434 0.091 0.616 0.259 0.254 0.262 0.239 DG(18:0_18:1) 0.332 0.656 0.266 1.33 0.202 0.964 0.249 1.465 0.265 0.82 0.311 1.026 0.332 0.607 0.271 2.35 0.355 0.539 0.426 0.895 0.292 1.297 0.355 1.575 0.336 1.27 0.321 1.174 0.322 1.113 0.243 1.788 0.632 0.629 0.645 0.691 DG(18:0_20:4) 0.114 0.838 0.197 2.177 0.049 1.434 0.178 2.412 0.126 0.932 0.285 1.205 0.096 0.726 0.057 3.932 0.177 0.773 0.384 1.289 0.203 1.959 0.191 2.529 0.24 2.131 0.275 1.92 0.168 1.793 0.141 2.81 0.859 0.769 0.775 0.761 DG(18:0_22:6) 0.768 0.037 0.273 0.023 0.747 0.951 0.048 0.932 0.043 0.018 1.449 0.022 0.044 0.119 0.927 0.011 0.371 1.159 0.107 0.161 0.124 1.096 0.151 0.096 0.232 1.339 0.204 0.119 0.641 0.185 0.406 0.128 0.418 0.385 0.398 0.408 DG(18:1/18:1) 0.383 1.1 0.32 1.049 0.073 0.702 0.288 1.217 0.572 2.317 0.593 1.388 0.126 0.521 0.086 1.518 0.776 1.063 0.693 1.191 0.256 1.084 0.238 1.191 1.134 2.26 0.576 1.251 0.37 1.207 0.248 1.268 0.83 0.795 0.728 0.817 DG(18:1_20:4) 0.062 0.219 0.098 0.399 0.016 0.26 0.077 0.416 0.084 0.401 0.16 0.339 0.035 0.154 0.017 0.66 0.116 0.195 0.201 0.295 0.092 0.33 0.078 0.434 0.27 0.688 0.178 0.462 0.091 0.39 0.05 0.455 0.232 0.203 0.211 0.21 GB3(d18:1/16:0) 0.03 0.031 0.041 0.051 0.031 0.032 0.032 0.033 0.021 0.025 0.06 0.07 0.031 0.028 0.013 0.016 0.019 0.024 0.072 0.08 0.026 0.032 0.022 0.032 0.019 0.021 0.029 0.041 0.024 0.034 0.023 0.029 0.034 0.036 0.028 0.027 GB3(d18:1/18:0(d3)) N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. GB3(d18:1/18:0) 0.005 0.014 0.013 0.02 0.014 0.018 0.012 0.014 0.007 0.011 0.018 0.021 0.011 0.016 0.009 0.005 0.009 0.009 0.018 0.017 0.011 0.013 0.011 0.012 0.004 0.008 0.012 0.02 0.013 0.017 0.009 0.009 0.01 0.01 0.016 0.013 GB3(d18:1/24:0) 0.022 0.025 0.029 0.048 0.022 0.067 0.036 0.052 0.014 0.027 0.051 0.072 0.049 0.071 0.011 0.015 0.011 0.022 0.073 0.084 0.039 0.065 0.025 0.039 0.014 0.013 0.002 0.046 0.058 0.073 0.035 0.044 0.042 0.04 0.056 0.042 GB3(d18:1/24:1) 0.072 0.071 0.055 0.081 0.067 0.131 0.089 0.09 0.047 0.072 0.094 0.107 0.095 0.107 0.023 0.03 0.032 0.063 0.104 0.124 0.071 0.088 0.043 0.066 0.037 0.048 0.054 0.07 0.089 0.095 0.056 0.06 0.066 0.056 0.077 0.088 GlcCer(d18:1(d5)/18:0) N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. GlcCer(d18:1/16:0(d3)) N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. Glucosylsphingosine(d5) N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. HexCer(d18:1/12:0) N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. HexCer(d18:1/16:0) 1.914 2.128 1.263 1.593 0.723 1.452 1.702 2.159 2.309 2.539 2.169 2.753 1.367 2.386 0.668 0.866 2.089 2.492 2.477 3.089 1.715 2.109 1.703 2.146 2.244 1.984 1.422 1.949 1.859 2.389 1.842 2.309 1.913 1.8 1.833 1.807 HexCer(d18:1/18:0) 0.576 6.127 0.446 5.255 0.265 5.082 0.766 5.224 0.66 6.737 0.891 5.788 0.605 6.02 0.25 5.244 0.625 6.438 1.144 5.692 0.776 5.738 0.775 5.729 0.649 6.911 0.628 6.276 1.006 6.19 0.964 6.541 2.665 2.558 2.453 2.487 HexCer(d18:1/22:0) 1.672 8.949 2.211 8.435 1.517 8.681 3.229 9.068 2.063 9.604 4.769 11.426 3.417 11.871 1.261 6.769 1.703 9.63 6.251 12.715 4.32 11.072 3.851 10.235 1.728 9.093 3.965 11.779 6.05 13.391 5.312 12.404 6.102 5.91 5.832 5.816 HexCer(d18:1/24:0) 2.134 15.683 3.062 14.428 2.096 14.379 4.457 14.771 2.62 16.639 7.034 19.267 4.866 19.465 1.849 10.579 2.113 17.015 9.405 21.214 6.077 18.218 5.317 15.956 2.495 16.294 6.187 19.176 8.251 19.763 8.13 19.453 9.968 9.646 9.222 9.201 HexCer(d18:1/24:1) 3.582 31.879 3.718 28.479 2.275 28.965 5.837 28.963 4.408 33.798 7.389 33.466 4.696 34.829 2.151 26.864 3.596 33.455 9.188 33.602 5.644 32.149 5.998 30.862 4.11 32.624 5.466 33.76 6.9 33.611 7.215 34.612 17.975 17.143 16.233 16.778 Hexosylsphingosine N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. LPC(16:0) 0.142 0.261 0.295 0.531 0.386 0.667 0.294 0.632 0.126 0.238 0.403 0.532 0.318 0.592 0.382 0.903 0.108 0.226 0.303 0.471 0.362 0.681 0.347 0.705 0.115 0.465 0.323 0.602 0.286 0.724 0.311 0.845 0.385 0.389 0.398 0.398 LPC(16:1) 0.011 0.012 0.011 0.013 0.011 0.012 0.01 0.012 0.016 0.013 0.02 0.018 0.01 0.013 0.014 0.014 0.012 0.014 0.02 0.018 0.011 0.012 0.011 0.012 0.007 0.012 0.012 0.011 0.01 0.01 0.009 0.013 0.012 0.013 0.014 0.014 LPC(18:0) 0.293 0.565 0.568 1.119 0.688 1.202 0.583 1.279 0.267 0.539 0.68 1.073 0.719 1.09 0.746 1.798 0.193 0.482 0.537 0.865 0.636 1.299 0.699 1.491 0.228 0.925 0.612 1.204 0.573 1.412 0.655 1.626 0.733 0.759 0.74 0.761 LPC(18:1(d7)) N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. LPC(18:1) 0.088 0.156 0.138 0.221 0.17 0.247 0.117 0.232 0.102 0.184 0.185 0.256 0.149 0.244 0.181 0.374 0.094 0.185 0.15 0.239 0.147 0.267 0.154 0.28 0.067 0.237 0.134 0.218 0.109 0.24 0.119 0.297 0.171 0.174 0.173 0.176 LPC(20:4) 0.026 0.041 0.043 0.038 0.039 0.051 0.042 0.051 0.023 0.03 0.049 0.068 0.052 0.088 0.037 0.031 0.02 0.034 0.068 0.064 0.055 0.064 0.046 0.047 0.017 0.032 0.044 0.041 0.075 0.042 0.045 0.046 0.045 0.05 0.053 0.056 LPC(22:6) 0.007 0.012 0.008 0.009 0.008 0.009 0.006 0.01 0.008 0.014 0.011 0.018 0.007 0.013 0.008 0.009 0.008 0.013 0.012 0.015 0.006 0.009 0.007 0.009 0.005 0.01 0.007 0.01 0.007 0.007 0.006 0.009 0.009 0.009 0.01 0.011 LPC(24:0) 0.004 0.05 0.007 0.037 0.004 0.043 0.01 0.045 0.004 0.072 0.012 0.082 0.008 0.084 0.003 0.025 0.003 0.068 0.018 0.088 0.009 0.067 0.009 0.048 0.004 0.05 0.013 0.069 0.013 0.078 0.013 0.061 0.026 0.026 0.027 0.027 LPC(24:1) 0.002 0.038 0.004 0.025 0.003 0.03 0.006 0.029 0.002 0.053 0.006 0.052 0.004 0.055 0.002 0.016 0.002 0.049 0.008 0.051 0.004 0.043 0.005 0.028 0.002 0.036 0.006 0.043 0.007 0.045 0.007 0.035 0.016 0.016 0.016 0.016 LPC(26:0) 0 0.003 0 0.002 0 0.002 0.001 0.002 0.001 0.004 0.001 0.004 0 0.003 0 0.002 0 0.003 0.001 0.004 0 0.003 0.001 0.002 0 0.003 0.001 0.003 0.001 0.003 0.001 0.003 0.001 0.001 0.001 0.001 LPC(26:1) 0 0.003 0.001 0.003 0 0.003 0.001 0.003 0 0.004 0.001 0.005 0 0.005 0 0.002 0 0.004 0.001 0.005 0 0.003 0 0.003 0.001 0.003 0.001 0.004 0.001 0.005 0.001 0.004 0.001 0.002 0.002 0.002 LacCer(d18:1/16:0) 0.138 0.189 0.079 0.117 0.088 0.159 0.093 0.13 0.153 0.229 0.062 0.099 0.073 0.172 0.06 0.102 0.172 0.277 0.058 0.09 0.084 0.163 0.075 0.112 0.175 0.245 0.051 0.087 0.067 0.146 0.074 0.111 0.102 0.104 0.102 0.103 LacCer(d18:1/17:0) N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. LacCer(d18:1/18:0) 0.014 0.063 0.008 0.045 0.008 0.054 0.017 0.051 0.017 0.072 0.012 0.053 0.01 0.077 0.006 0.036 0.018 0.073 0.014 0.052 0.011 0.061 0.011 0.053 0.018 0.064 0.008 0.052 0.014 0.072 0.013 0.057 0.031 0.029 0.028 0.027 LacCer(d18:1/24:0) 0.034 0.073 0.032 0.066 0.022 0.072 0.057 0.085 0.045 0.094 0.046 0.1 0.036 0.113 0.011 0.028 0.046 0.096 0.067 0.113 0.047 0.095 0.039 0.075 0.051 0.078 0.036 0.08 0.044 0.13 0.05 0.09 0.06 0.055 0.057 0.056 LacCer(d18:1/24:1) 0.048 0.131 0.029 0.075 0.024 0.09 0.053 0.101 0.065 0.172 0.039 0.124 0.027 0.139 0.012 0.037 0.067 0.175 0.049 0.119 0.034 0.11 0.034 0.087 0.069 0.134 0.026 0.082 0.035 0.133 0.038 0.094 0.071 0.067 0.068 0.063 Lactosylsphingosine N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. MG(16:0) 127.892 156.286 142.75 148.222 129.794 185.499 120.8 187.579 114.643 164.92 161.725 152.836 170.274 163.325 141.485 173.155 215.373 223.38 126.046 143.373 144.399 284.339 177.771 167.345 142.87 119.034 108.872 118.37 109.261 95.875 133.741 140.057 149.005 158.461 160.658 171.795 MG(16:1) N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. MG(18:0) 560.565 648.691 567.421 610.379 523.179 670.184 491.597 721.036 505.571 627.844 549.35 633.644 750.419 610.571 521.378 700.763 821.325 788.408 518.993 528.278 593.572 1088.906 696.611 691.278 484.19 421.55 376.252 438.425 415.044 374.303 483.834 531.533 544.378 582.754 570.612 630.14 MG(18:1(d7)) N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. MG(18:1) 17.454 22.402 17.46 21.069 15.596 21.207 15.128 23.471 16.251 24.082 16.155 21.592 26.643 20.877 17.66 25.576 27.588 27.9 18.481 17.241 20.612 37.914 22.149 23.48 23.039 19.796 17.044 19.872 14.483 16.642 22.286 21.728 20.761 21.306 20.926 23.385 MG(20:4) N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N-Oleoylethanolamine 8.36E-05 0 0 0 6.23E-05 0 0 0 0 0 0 0 0 0 7.36E-05 0 9.39E-05 0 0 0 8.19E-05 0 0 0.001 0 0.001 0 0 9.85E-05 0 0 0 0 0 0 0 N-Palmitoyl-O-phosphocholineserine N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. PC(15:0/18:1(d7)) N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. PC(16:0/5:0(CHO)) 0.006 0.004 0.007 0.007 0.007 0.006 0.011 0.007 0.002 0.002 0.007 0.004 0.007 0.006 0.005 0.003 0.001 0.001 0.007 0.003 0.008 0.003 0.006 0.003 0.003 0.003 0.007 0.005 0.009 0.005 0.008 0.008 0.007 0.007 0.006 0.006 PC(16:0/9:0(CHO)) 0.054 0.046 0.029 0.045 0.023 0.033 0.029 0.028 0.03 0.032 0.025 0.022 0.018 0.026 0.013 0.019 0.016 0.019 0.025 0.014 0.02 0.012 0.011 0.013 0.024 0.026 0.028 0.026 0.021 0.019 0.018 0.027 0.026 0.026 0.024 0.024 PC(16:0/9:0(COOH)) 0.003 0.003 0.003 0.003 0.003 0.003 0.003 0.003 0.002 0.002 0.002 0.002 0.002 0.002 0.001 0.002 0.001 0.005 0.002 0.002 0.002 0.001 0.001 0.002 0.002 0.001 0.002 0.002 0.003 0.002 0.003 0.003 0.003 0.002 0.002 0.002 PC(18:0/20:4(OH[S])) N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. PC(18:0/20:4(OOH[S])) N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. PC(34:1) 4.09 4.62 3.408 4.448 2.064 4.056 3.631 4.405 4.409 5.021 4.632 5.166 3.232 4.167 1.941 3.982 4.264 4.662 4.937 5.47 3.464 4.309 3.509 4.502 4.084 4.419 3.991 4.907 3.838 4.344 3.695 4.681 4.185 4.181 4.151 4.272 PC(36:1) 1.113 3.268 0.963 3.199 0.532 3.149 1.189 3.229 1.322 3.57 1.839 3.738 1.026 3.269 0.399 3.133 1.26 3.316 2.16 3.862 1.204 3.212 1.133 3.325 1.28 3.434 1.572 3.749 1.544 3.342 1.368 3.613 2.423 2.355 2.364 2.413 PC(36:2) 3.027 4.081 1.633 2.862 0.713 2.269 2.029 2.999 3.949 5.07 3.284 4.292 1.508 2.692 0.687 1.996 3.701 4.737 3.68 4.617 1.792 2.673 1.822 2.948 3.232 3.925 2.487 3.741 2.27 3.024 2.214 3.298 2.917 2.908 2.927 2.978 PC(36:4) 2.235 2.389 2.711 3.132 2.187 3.354 3.398 3.641 2.064 2.573 3.725 3.833 3.366 3.66 2.431 3.119 1.813 2.292 4.091 4.244 3.435 3.622 3.659 3.814 2.28 2.602 3.395 3.719 3.826 3.741 3.824 3.975 3.33 3.331 3.331 3.356 PC(38:4) 0.805 1.018 1.2 1.404 0.803 1.601 1.804 1.988 0.731 1.127 1.983 2.223 1.723 2.011 0.943 1.297 0.632 0.809 2.409 2.472 1.807 1.955 2.021 2.15 0.773 1.034 1.931 2.117 2.318 2.206 2.346 2.397 1.747 1.711 1.748 1.786 PC(38:6) 0.459 0.573 0.362 0.633 0.154 0.402 0.56 0.712 0.543 0.698 0.976 1.089 0.33 0.516 0.152 0.346 0.429 0.592 1.173 1.232 0.326 0.472 0.411 0.652 0.474 0.595 0.642 0.885 0.486 0.645 0.561 0.813 0.594 0.596 0.572 0.57 PC(40:5) 0.213 0.254 0.254 0.338 0.136 0.309 0.352 0.444 0.248 0.33 0.485 0.6 0.328 0.407 0.141 0.234 0.204 0.259 0.578 0.662 0.334 0.424 0.371 0.498 0.223 0.261 0.412 0.551 0.504 0.49 0.439 0.585 0.382 0.375 0.379 0.384 PC(40:6) 0.26 0.38 0.244 0.426 0.121 0.307 0.359 0.507 0.298 0.449 0.55 0.667 0.232 0.339 0.109 0.296 0.239 0.379 0.605 0.728 0.229 0.352 0.298 0.467 0.259 0.403 0.445 0.593 0.345 0.461 0.408 0.566 0.38 0.38 0.374 0.374 PC(O-16:0/0:0) 0.001 0.001 0.001 0.001 0.001 0.001 0.001 0.001 0.001 0.001 0.001 0.001 0.001 0.001 0.001 0.002 0.001 0.001 0.001 0.001 0.001 0.001 0.001 0.001 0.001 0.001 0.001 0.001 0.001 0.001 0.001 0.002 0.001 0.001 0.001 0.001 PC(O-16:0/2:0) 0.048 0.074 0.056 0.069 0.048 0.087 0.045 0.053 0.03 0.046 0.036 0.04 0.044 0.089 0.033 0.032 0.016 0.034 0.037 0.036 0.049 0.038 0.029 0.029 0.032 0.04 0.048 0.045 0.062 0.059 0.048 0.064 0.05 0.052 0.052 0.05 PC(O-18:0/2:0) 0.006 0.011 0.011 0.02 0.011 0.022 0.01 0.02 0.004 0.01 0.009 0.015 0.01 0.019 0.01 0.024 0.003 0.008 0.008 0.011 0.009 0.018 0.009 0.018 0.004 0.015 0.01 0.019 0.01 0.02 0.011 0.025 0.012 0.011 0.012 0.011 PE(15:0/18:1(d7)) N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. PE(18:0/20:4(OH[S])) N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. PE(18:0/20:4(OOH[S])) N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. PE(34:1) 7.696 8.244 3.86 8.586 1.405 5.563 4.84 8.529 9.663 9.423 9.36 10.122 2.764 4.367 1.269 7.061 8.01 9.67 10.418 11.591 3.244 6.006 3.574 8.041 6.672 9.773 5.062 9.144 4.002 6.474 4.346 9.462 6.701 6.601 6.375 6.359 PE(36:1) 15.004 22.944 9.404 22.769 4.425 16.921 11.941 22.978 17.578 27.193 20.279 29.046 8.746 17.948 3.66 16.955 15.549 26.15 23.633 31.855 10.107 19.468 10.132 22.307 15.178 25.395 13.683 25.766 13.701 21.674 12.757 26.749 16.786 17.009 16.568 16.927 PE(36:2) 10.678 13.551 3.698 13.947 1.33 9.535 5.302 14.192 14.85 16.611 9.55 14.841 2.593 7.454 1.184 14.112 13.569 17.823 11.133 16.313 3.217 10.691 3.83 15.063 10.443 17.844 5.164 15.354 4.252 11.312 4.97 17.19 9.591 9.43 9.206 9.242 PE(36:4) 1.67 1.749 1.086 2.36 0.468 1.581 2.148 2.815 1.599 1.737 3.206 3.292 1.278 1.694 0.624 1.894 1.187 1.648 3.797 4.002 1.531 2.032 1.775 3.026 1.321 2.157 1.847 2.942 2.07 2.317 2.228 3.522 2.086 2.033 2.011 1.949 PE(38:4) 16.827 17.528 12.766 18.49 5.621 14.333 18.135 21.515 18.997 19.995 27.91 28.103 15.838 18.688 7.245 11.941 15.614 19.306 32.853 33.799 16.978 18.826 18.532 22.571 17.209 18.39 20.622 23.633 20.949 21.189 22.535 25.799 19.733 19.96 19.544 19.928 PE(38:5) 3.393 4.034 1.758 4.942 0.67 3.456 3.013 5.471 4.268 4.78 5.181 6.378 1.876 3.279 0.829 4.799 3.399 4.68 5.911 7.249 2.147 4.276 2.626 5.686 3.295 5.855 2.76 5.915 3.019 4.673 3.206 6.69 3.872 3.881 3.72 3.551 PE(38:6) 2.157 1.853 1.416 2.497 0.57 1.406 2.581 2.981 2.139 1.852 4.53 3.534 1.292 1.213 0.698 2.254 1.778 1.918 4.996 4.362 1.389 1.733 2.083 2.734 1.638 2.108 2.251 2.829 1.952 1.94 2.455 3.31 2.284 2.206 2.224 2.292 PE(40:4) 4.263 4.075 3.122 4.289 1.134 2.548 3.446 4.254 4.715 5.193 7.777 7.187 3.619 3.495 1.287 2.516 4.179 4.926 9.725 9.32 3.919 3.797 3.852 4.773 4.544 4.591 5.41 6.172 5.717 4.42 4.786 5.716 4.615 4.617 4.772 4.675 PE(40:5) N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. PE(40:6) 5.773 5.264 3.373 5.998 1.174 3.605 4.909 6.933 6.468 6.462 10.086 9.57 3.021 3.487 1.172 4.281 5.508 6.432 10.994 10.712 3.239 4.592 4.272 6.241 5.015 6.17 5.67 7.182 5.413 5.285 5.943 7.602 5.795 5.794 5.665 5.659 PE(40:7) 3.549 2.639 1.353 2.173 0.451 0.987 2.069 2.222 4.589 3.36 4.143 3.342 1.06 0.93 0.54 1.472 3.692 3.443 4.261 3.864 1.071 1.293 1.425 2.026 3.047 2.823 1.969 2.526 1.508 1.411 1.91 2.229 2.287 2.246 2.272 2.308 Palmitoylethanolamine 2.79E-05 0 3.20E-05 0 N.D. 0 2.48E-05 0 7.11E-05 7.89E-05 3.37E-05 7.16E-05 2.21E-05 8.67E-05 N.D. 0 N.D. 5.35E-05 5.09E-05 0 3.15E-05 0 6.13E-05 0 3.04E-05 0 N.D. 0 4.76E-05 0 3.23E-05 0 9.14E-05 0 0 8.86E-05 SM(d18:1(d9)/18:1) N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. SM(d18:1/16:0) 6.85 6.29 4.632 5.177 3.051 3.935 5.047 5.311 7.554 7.517 7.095 7.17 4.042 4.47 2.885 3.264 6.989 7.081 7.583 7.572 4.078 4.319 4.582 4.876 6.391 5.973 5.14 5.56 4.551 4.648 4.73 5.002 5.495 5.531 5.567 5.421 SM(d18:1/18:0) 1.731 2.305 1.174 2.027 0.472 1.458 1.641 2.198 2.08 2.765 2.71 2.971 1.082 1.638 0.435 1.43 1.829 2.741 2.967 3.148 1.168 1.752 1.366 2.097 1.743 2.486 1.675 2.319 1.555 1.985 1.648 2.369 1.93 1.861 1.898 1.888 SM(d18:1/24:0) 2.073 2.986 1.868 2.922 0.679 1.814 2.13 2.719 2.55 3.567 4.382 5.021 1.477 2.396 0.561 1.114 2.182 3.52 5.402 5.583 1.621 2.336 1.822 2.628 2.198 2.974 3.08 3.909 2.281 2.761 2.4 3.169 2.914 2.841 2.946 2.869 SM(d18:1/24:1) 5.995 7.438 4.745 6.386 1.913 4.294 5.408 6.559 7.259 8.585 8.78 9.328 3.412 5.026 1.771 3.53 6.483 8.203 9.732 9.922 3.556 4.97 4.567 5.928 5.955 6.804 5.944 7.056 4.396 5.505 5.131 6.385 6.455 6.245 6.305 6.183 Sitosteryl hexoside N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. Sphinganine N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. Sphinganine 1-phosphate 0.009 0.117 0.004 0.053 0.001 0.062 0.007 0.041 0.006 0.185 0.008 0.12 0.001 0.073 0.002 0.021 0.005 0.093 0.007 0.089 0.004 0.1 0.004 0.056 0.006 0.23 0.008 0.174 0.006 0.129 0.003 0.019 0.029 0.038 0.037 0.035 Sphingosine 1.376 25.931 1.478 13.438 1.38 17.049 1.053 11.039 0.967 28.719 1.341 26.1 0.484 26.806 0.345 4.666 0.657 23.46 1.426 18.171 1.229 18.193 0.675 10.658 0.744 19.453 0.86 15.697 1.134 19.253 0.988 13.731 7.233 6.441 7.136 7.699 Sphingosine 1-phosphate 0.006 0.079 0.005 0.059 0.004 0.056 0.002 0.038 0.004 0.132 0.004 0.098 0.004 0.064 0.002 0.025 0.005 0.063 0.008 0.068 0.006 0.097 0.009 0.049 0.005 0.18 0.006 0.144 0.006 0.109 0.005 0.015 0.025 0.025 0.029 0.028 Sphingosine 1-phosphate-d7 N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. Sphingosine 1-phosphocholine N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. Sphingosine(d17:1) N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. TG(15:0/18:1(d7)/15:0) N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. TG(18:0_36:2) 0.181 0.644 0.134 0.293 0.049 0.268 0.088 0.266 0.166 0.882 0.195 0.603 0.08 0.449 0.02 0.089 0.239 0.863 0.244 0.644 0.147 0.428 0.081 0.251 0.309 0.835 0.157 0.419 0.155 0.488 0.076 0.232 0.303 0.286 0.277 0.29 TG(18:1_34:2) 0.866 1.435 0.357 0.639 0.077 0.312 0.227 0.401 0.929 1.863 0.605 1.134 0.115 0.452 0.046 0.108 1.231 1.775 0.682 1.194 0.201 0.49 0.138 0.304 1.074 1.469 0.361 0.569 0.185 0.501 0.137 0.225 0.627 0.613 0.622 0.632 TG(18:1_34:3) 0.184 0.217 0.066 0.097 0.01 0.032 0.039 0.055 0.232 0.278 0.12 0.174 0.02 0.043 0.007 0.011 0.263 0.294 0.156 0.208 0.031 0.049 0.023 0.039 0.238 0.241 0.069 0.078 0.029 0.053 0.024 0.029 0.11 0.102 0.103 0.101 TG(20:4_32:1) 0.095 0.081 0.075 0.075 0.011 0.023 0.028 0.041 0.076 0.084 0.077 0.107 0.017 0.029 0.007 0.011 0.107 0.096 0.105 0.142 0.03 0.039 0.02 0.031 0.166 0.152 0.058 0.06 0.026 0.038 0.018 0.022 0.057 0.054 0.056 0.055 TG(20:4_34:2) 0.086 0.076 0.06 0.066 0.009 0.018 0.023 0.037 0.072 0.084 0.066 0.099 0.017 0.024 0.006 0.01 0.099 0.094 0.087 0.127 0.027 0.035 0.018 0.03 0.177 0.163 0.057 0.059 0.028 0.036 0.018 0.019 0.054 0.05 0.052 0.05 TG(20:4_34:3) 0.02 0.011 0.015 0.012 0.002 0.003 0.007 0.007 0.02 0.01 0.022 0.018 0.005 0.003 0.002 0.002 0.022 0.013 0.031 0.027 0.007 0.005 0.006 0.006 0.036 0.022 0.016 0.01 0.008 0.005 0.007 0.004 0.012 0.012 0.011 0.011 TG(20:4_36:0) 0.019 0.071 0.024 0.048 0.007 0.034 0.013 0.035 0.011 0.073 0.023 0.08 0.012 0.05 0.003 0.012 0.018 0.075 0.035 0.099 0.021 0.051 0.013 0.033 0.03 0.091 0.03 0.069 0.023 0.058 0.014 0.032 0.032 0.034 0.034 0.034 TG(20:4_36:2) 0.148 0.273 0.116 0.188 0.019 0.085 0.043 0.114 0.119 0.294 0.116 0.314 0.035 0.127 0.011 0.036 0.191 0.329 0.143 0.338 0.064 0.146 0.035 0.106 0.339 0.492 0.114 0.216 0.064 0.155 0.038 0.08 0.138 0.135 0.138 0.129 TG(20:4_36:3) 0.069 0.06 0.078 0.08 0.014 0.028 0.026 0.048 0.052 0.055 0.067 0.115 0.026 0.031 0.008 0.017 0.074 0.069 0.089 0.141 0.04 0.051 0.022 0.045 0.156 0.151 0.069 0.069 0.042 0.048 0.024 0.032 0.06 0.053 0.055 0.054 TG(22:6_36:2) 0.062 0.259 0.026 0.095 0.004 0.035 0.013 0.056 0.058 0.315 0.044 0.173 0.007 0.055 0.002 0.009 0.089 0.308 0.049 0.177 0.011 0.048 0.008 0.041 0.13 0.342 0.033 0.115 0.013 0.06 0.011 0.031 0.072 0.063 0.066 0.068 TG(22:6_38:1) 0.002 0.021 0.001 0.008 0 0.007 0.001 0.007 0.002 0.021 0.001 0.013 0.001 0.009 0 0.001 0.002 0.02 0.001 0.011 0.001 0.007 0 0.005 0.003 0.022 0.001 0.01 0.001 0.007 0.001 0.004 0.005 0.005 0.005 0.005 TG(22:6_38:2) 0.007 0.04 0.003 0.015 0.001 0.009 0.001 0.012 0.006 0.048 0.004 0.028 0.001 0.013 0 0.002 0.008 0.044 0.004 0.025 0.001 0.011 0.001 0.008 0.011 0.048 0.003 0.021 0.001 0.012 0.001 0.007 0.01 0.011 0.01 0.01 lyso-GB3 N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. lyso-GB3-d7 N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. lyso-GB4 N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name Expected RT MS1_mz MS2_mz 1-O-Palmitoyl-Cer(d18:1/18:0) 8.1 786.8 502.5 24-Hydroxycholesterol 2 385.3 367.3 24-Hydroxycholesterol(d7) 2 392.3 367.3 3-O-SulfoLacCer(d18:1/18:0) 5.2 970.8 548.5 4-beta-Hydroxycholesterol 2.14 420.3 385.3 7-keto-Cholesterol 3.2 401.3 383.3 CE HETE 7.27 706.6 369.2 CE HODE 7.32 682.6 369.2 CE HpODE 6.76 698.6 369.2 CE oxoHETE 6.65 704.6 369.2 CE oxoODE 6.73 680.6 369.2 CE(16:1) 8.42 640.6 369.3 CE(18:1(d7)) 8.64 675.2 369.4 CE(18:1) 8.64 668.6 369.3 CE(18:2) 8.43 666.6 369.3 CE(20:4) 8.3 690.6 369.3 CE(20:5) 8.09 688.6 369.3 CE(22:6) 8.16 714.6 369.3 Cer(d18:0/16:0) 6.29 540.6 284.3 Cer(d18:0/18:0) 6.79 568.7 284.3 Cer(d18:0/24:0) 7.81 652.9 284.3 Cer(d18:0/24:1) 7.07 650.9 284.4 Cer(d18:1/16:0(d7)) 5.71 545.5 271.4 Cer(d18:1/16:0) 5.89 538.5 264.3 Cer(d18:1/18:0) 6.39 566.6 264.3 Cer(d18:1/24:0) 7.41 650.6 264.3 Cer(d18:1/24:1) 7.07 648.6 264.3 Cholesterol 5.13 369.3 369.3 Cholesterol(d7) 5.13 376.2 376.2 Cholesteryl hexoside 3.91 566.6 369.3 Coenzyme Q10 7.75 863.3 197.2 DG(15:0/18:1(d7)) 6.08 605.6 346.5 DG(16:0_18:1) 6.5 612.4 313.3 DG(16:0_20:4) 6.1 634.5 313.3 DG(18:0_18:1) 7.02 640.4 341.3 DG(18:0_20:4) 6.6 662.5 341.3 DG(18:0_22:6) 6.1 686.6 341.3 DG(18:1_20:4) 6.12 660.5 339.3 DG(18:1/18:1) 6.66 638.4 339.3 GB3(d18:1/16:0) 5.2 1025 520.5 GB3(d18:1/18:0(d3)) 5.51 1056 551.6 GB3(d18:1/18:0) 5.51 1053 548.6 GB3(d18:1/24:0) 6.95 1137 632.6 GB3(d18:1/24:1) 6.55 1135 630.6 GlcCer(d18:1(d5)/18:0) 6 733.6 269.3 GlcCer(d18:1/16:0(d3)) 5.5 703.7 264.3 Glucosylsphingosine(d5) 1.3 467.2 269.3 HexCer(d18:1/12:0) 4.26 644.5 264.3 HexCer(d18:1/16:0) 5.5 700.6 264.3 HexCer(d18:1/18:0) 6 728.6 264.3 HexCer(d18:1/22:0) 6.7 784.7 264.4 HexCer(d18:1/24:0) 7.14 812.7 264.3 HexCer(d18:1/24:1) 6.78 810.7 264.3 Hexosylsphingosine 1.3 462.3 264.2 LacCer(d18:1/16:0) 5.31 862.6 264.3 LacCer(d18:1/17:0) 5.51 876.6 264.3 LacCer(d18:1/18:0) 5.84 890.7 264.3 LacCer(d18:1/24:0) 7 974.8 264.3 LacCer(d18:1/24:1) 6.64 972.7 264.3 Lactosylsphingosine 1.4 624.4 264.3 LPC(16:0) 1.85 496.3 184.1 LPC(16:1) 1.48 494.5 184.1 LPC(18:0) 2.47 524.3 184.1 LPC(18:1(d7)) 1.91 529.3 184.1 LPC(18:1) 1.9 522.3 184.1 LPC(20:4) 1.45 544.3 184.1 LPC(22:6) 1.35 568.3 184.1 LPC(24:0) 4.53 608.5 184.1 LPC(24:1) 3.8 606.5 184.1 LPC(26:0) 5.2 636.5 104.1 LPC(26:1) 4.7 634.5 104.1 lyso-GB3 1.21 786.6 264.3 lyso-GB3-d7 1.21 793.5 271.3 lyso-GB4 1.04 990.6 264.3 MG(16:0) 2.48 348.3 239.3 MG(16:1) 2 346.3 237.3 MG(18:0) 3.32 376.3 267.3 MG(18:1(d7)) 2.5 381.3 272.5 MG(18:1) 2.56 374.3 265.3 MG(20:4) 1.96 396.3 287.3 N-Oleoylethanolamine 2.2 326.3 62.1 N-Palmitoyl-O-phosphocholineserine 509.5 184.1 Palmitoylethanolamine 2.2 300.3 62.1 PC(15:0/18:1(d7)) 5.37 754.6 184.1 PC(16:0/5:0(CHO)) 2 594.5 184.1 PC(16:0/9:0(CHO)) 2 650.4 184.1 PC(16:0/9:0(COOH)) 2.1 666.4 184.1 PC(18:0/20:4(OH[S])) 4.12 826.6 184.1 PC(18:0/20:4(OOH[S])) 4.11 842.6 184.1 PC(34:1) 5.7 760.6 184.1 PC(36:1) 6.1 788.6 184.1 PC(36:2) 5.69 786.6 184.1 PC(36:4) 5.2 782.6 184.1 PC(38:4) 5.48 810.6 184.1 PC(38:6) 4.92 806.6 184.1 PC(40:5) 5.9 836.6 184.1 PC(40:6) 5.46 834.6 184.1 PC(O-16:0/0:0) 1.6 482.3 184.1 PC(O-16:0/2:0) 1.8 524.3 184.2 PC(O-18:0/2:0) 2.37 552.5 184.1 PE(15:0/18:1(d7)) 5.52 711.6 570.5 PE(18:0/20:4(OH[S])) 4.28 784.5 643.4 PE(18:0/20:4(OOH[S])) 4.23 800.5 659.4 PE(34:1) 5.8 718.6 577.6 PE(36:1) 6.22 746.6 605.5 PE(36:2) 5.8 744.6 603.5 PE(36:4) 5.2 740.6 599.5 PE(38:4) 5.77 768.6 627.5 PE(38:5) 5.2 766.6 625.5 PE(38:6) 5.07 764.6 623.5 PE(40:4) 6 796.6 655.5 PE(40:5) 5.8 794.6 635.5 PE(40:6) 5.59 792.6 651.5 PE(40:7) 5 790.6 649.5 Sitosteryl hexoside 4.36 594.6 397.4 SM(d18:1(d9)/18:1) 5.33 738.7 184.1 SM(d18:1/16:0) 5.33 703.6 184.1 SM(d18:1/18:0) 5.89 731.6 184.1 SM(d18:1/24:0) 7.07 815.7 184.1 SM(d18:1/24:1) 6.5 813.7 184.1 Sphinganine 1.88 302.2 284.3 Sphinganine 1-phosphate 1.68 382.3 284.3 Sphingosine 1.68 300.2 264.3 Sphingosine 1-phosphate 1.6 380.3 264.3 Sphingosine 1-phosphate-d7 1.6 387.3 271.3 Sphingosine 1-phosphocholine 1.63 465.5 184.1 Sphingosine(d17:1) 1.33 286.2 250.3 TG(15:0/18:1(d7)/15:0) 8.26 829.8 570.8 TG(18:0_36:2) 8.63 904.7 603.4 TG(18:1_34:2) 8.27 874.7 575.4 TG(18:1_34:3) 8.09 872.7 573.4 TG(20:4_32:1) 7.99 870.6 549.3 TG(20:4_34:2) 7.99 896.6 575.3 TG(20:4_34:3) 7.63 894.6 573.3 TG(20:4_36:0) 8.42 928.8 607.5 TG(20:4_36:2) 8.22 924.7 603.4 TG(20:4_36:3) 7.98 922.7 601.4 TG(22:6_36:2) 8 948.7 603.4 TG(22:6_38:1) 8.29 978.7 633.4 TG(22:6_38:2) 8.1 976.7 631.4 METABOLITES_END #END