#METABOLOMICS WORKBENCH Jung_DNLI_20250905_111043 DATATRACK_ID:6386 STUDY_ID:ST004205 ANALYSIS_ID:AN006993 PROJECT_ID:PR002651
VERSION             	1
CREATED_ON             	September 19, 2025, 6:13 pm
#PROJECT
PR:PROJECT_TITLE                 	GPR34 regulates microglia state and loss-of-function rescues TREM2 metabolic
PR:PROJECT_TITLE                 	dysfunction
PR:PROJECT_TYPE                  	cellular study
PR:PROJECT_SUMMARY               	Microglia are implicated in modifying neurodegenerative disease risk in the
PR:PROJECT_SUMMARY               	central nervous system (CNS). GPR34 is a microglia-enriched G-protein coupled
PR:PROJECT_SUMMARY               	receptor that detects cytotoxic lipids upregulated in Alzheimer’s Disease
PR:PROJECT_SUMMARY               	(AD). Since dysregulated lipid metabolism occurs in disease, we hypothesized
PR:PROJECT_SUMMARY               	GPR34 could act with other lipid sensors, such as TREM2, to regulate microglial
PR:PROJECT_SUMMARY               	function. Here, we report that GPR34 knockout (KO) rescues dysregulated
PR:PROJECT_SUMMARY               	cholesterol metabolism in TREM2 KO iPSC-derived microglia (iMG) and alone
PR:PROJECT_SUMMARY               	promotes fatty acid catabolism without the proton leak observed in TREM2 KO.
PR:PROJECT_SUMMARY               	Loss of GPR34 downregulated ERK signaling, while its agonism promoted
PR:PROJECT_SUMMARY               	interaction with and activation of ERK. In healthy and amyloid mouse models,
PR:PROJECT_SUMMARY               	Gpr34 KO accelerated the conversion of homeostatic microglia to
PR:PROJECT_SUMMARY               	disease-associated microglia (DAM) states. Additionally, in Gpr34 KO amyloid
PR:PROJECT_SUMMARY               	mouse brain, the frequency of large plaques was increased compared to WT,
PR:PROJECT_SUMMARY               	indicating that Gpr34 KO microglia may promote amyloid aggregation. Overall, our
PR:PROJECT_SUMMARY               	data suggest GPR34 as a therapeutic target for modulating microglial function to
PR:PROJECT_SUMMARY               	slow AD progression.
PR:INSTITUTE                     	Denali Therapeutics
PR:LAST_NAME                     	Suh
PR:FIRST_NAME                    	Jung
PR:ADDRESS                       	161 Oyster Point Blvd, South San Francisco, California, 94080, USA
PR:EMAIL                         	suh@dnli.com
PR:PHONE                         	+1 6507973837
#STUDY
ST:STUDY_TITLE                   	Lipidomic/Metabolomic Characterization of GPR34 KO, WT, TREM2 KO, and
ST:STUDY_TITLE                   	GPR34/TREM2 dKO iPSC1-derived microglia
ST:STUDY_SUMMARY                 	GPR34 is a microglia-enriched receptor that senses cytotoxic lipids in
ST:STUDY_SUMMARY                 	Alzheimer’s disease and may interact with other lipid sensors like TREM2 to
ST:STUDY_SUMMARY                 	regulate metabolism. Using targeted lipid and metabolite profiling of
ST:STUDY_SUMMARY                 	iPSC-derived microglia with GPR34 KO, TREM2 KO, or GPR34/TREM2 double KO, we
ST:STUDY_SUMMARY                 	found that GPR34 deletion restores cholesterol metabolism defects in TREM2 KO
ST:STUDY_SUMMARY                 	cells and independently enhances fatty acid catabolism.
ST:INSTITUTE                     	Denali Therapeutics
ST:LAST_NAME                     	Suh
ST:FIRST_NAME                    	Jung
ST:ADDRESS                       	161 Oyster Point Blvd
ST:EMAIL                         	suh@dnli.com
ST:PHONE                         	+1 6507973837
#SUBJECT
SU:SUBJECT_TYPE                  	Human
SU:SUBJECT_SPECIES               	Homo sapiens
SU:TAXONOMY_ID                   	9606
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data
SUBJECT_SAMPLE_FACTORS           	Pool_1	HSA-000028400	Genotype:NA | Treatment:NA | Sample source:iMicroglia	RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_001; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_001; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_001
SUBJECT_SAMPLE_FACTORS           	WT3 Myelin	HSA-000028164	Genotype:WT | Treatment:Myelin | Sample source:iMicroglia	RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_002; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_002; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_002
SUBJECT_SAMPLE_FACTORS           	GPR34KO4 Myelin	HSA-000028179	Genotype:GPR34-KO | Treatment:Myelin | Sample source:iMicroglia	RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_004; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_004; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_004
SUBJECT_SAMPLE_FACTORS           	dKO A4 4 Myelin	HSA-000028185	Genotype:GPR34/TREM2-KO | Treatment:Myelin | Sample source:iMicroglia	RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_005; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_005; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_005
SUBJECT_SAMPLE_FACTORS           	dKO A4 3 Myelin	HSA-000028173	Genotype:GPR34/TREM2-KO | Treatment:Myelin | Sample source:iMicroglia	RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_006; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_006; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_006
SUBJECT_SAMPLE_FACTORS           	TREM2KO1 Myelin	HSA-000028146	Genotype:TREM2-KO | Treatment:Myelin | Sample source:iMicroglia	RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_007; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_007; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_007
SUBJECT_SAMPLE_FACTORS           	GPR34KO1 Myelin	HSA-000028143	Genotype:GPR34-KO | Treatment:Myelin | Sample source:iMicroglia	RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_009; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_009; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_009
SUBJECT_SAMPLE_FACTORS           	dKO A4 4 PBS	HSA-000028184	Genotype:GPR34/TREM2-KO | Treatment:PBS | Sample source:iMicroglia	RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_010; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_010; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_010
SUBJECT_SAMPLE_FACTORS           	TREM2KO3 PBS	HSA-000028169	Genotype:TREM2-KO | Treatment:PBS | Sample source:iMicroglia	RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_011; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_011; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_011
SUBJECT_SAMPLE_FACTORS           	GPR34KO3 Myelin	HSA-000028167	Genotype:GPR34-KO | Treatment:Myelin | Sample source:iMicroglia	RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_012; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_012; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_012
SUBJECT_SAMPLE_FACTORS           	TREM2KO2 PBS	HSA-000028157	Genotype:TREM2-KO | Treatment:PBS | Sample source:iMicroglia	RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_013; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_013; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_013
SUBJECT_SAMPLE_FACTORS           	TREM2KO4 Myelin	HSA-000028182	Genotype:TREM2-KO | Treatment:Myelin | Sample source:iMicroglia	RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_016; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_016; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_016
SUBJECT_SAMPLE_FACTORS           	Pool_2	HSA-000028401	Genotype:NA | Treatment:NA | Sample source:iMicroglia	RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_017; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_017; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_017
SUBJECT_SAMPLE_FACTORS           	WT3 PBS	HSA-000028163	Genotype:WT | Treatment:PBS | Sample source:iMicroglia	RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_019; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_019; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_019
SUBJECT_SAMPLE_FACTORS           	TREM2KO2 Myelin	HSA-000028158	Genotype:TREM2-KO | Treatment:Myelin | Sample source:iMicroglia	RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_020; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_020; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_020
SUBJECT_SAMPLE_FACTORS           	dKO A4 2 Myelin	HSA-000028161	Genotype:GPR34/TREM2-KO | Treatment:Myelin | Sample source:iMicroglia	RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_021; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_021; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_021
SUBJECT_SAMPLE_FACTORS           	GPR34KO3 PBS	HSA-000028166	Genotype:GPR34 KO | Treatment:PBS | Sample source:iMicroglia	RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_022; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_022; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_022
SUBJECT_SAMPLE_FACTORS           	WT2 PBS	HSA-000028151	Genotype:WT | Treatment:PBS | Sample source:iMicroglia	RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_023; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_023; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_023
SUBJECT_SAMPLE_FACTORS           	GPR34KO4 PBS	HSA-000028178	Genotype:GPR34-KO | Treatment:PBS | Sample source:iMicroglia	RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_024; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_024; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_024
SUBJECT_SAMPLE_FACTORS           	TREM2KO3 Myelin	HSA-000028170	Genotype:TREM2-KO | Treatment:Myelin | Sample source:iMicroglia	RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_026; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_026; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_026
SUBJECT_SAMPLE_FACTORS           	WT2 Myelin	HSA-000028152	Genotype:WT | Treatment:Myelin | Sample source:iMicroglia	RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_027; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_027; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_027
SUBJECT_SAMPLE_FACTORS           	GPR34KO1 PBS	HSA-000028142	Genotype:GPR34-KO | Treatment:PBS | Sample source:iMicroglia	RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_028; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_028; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_028
SUBJECT_SAMPLE_FACTORS           	WT1 Myelin	HSA-000028140	Genotype:WT | Treatment:Myelin | Sample source:iMicroglia	RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_031; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_031; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_031
SUBJECT_SAMPLE_FACTORS           	dKO A4 1 Myelin	HSA-000028149	Genotype:GPR34/TREM2-KO | Treatment:Myelin | Sample source:iMicroglia	RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_032; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_032; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_032
SUBJECT_SAMPLE_FACTORS           	Pool_3	HSA-000028402	Genotype:NA | Treatment:NA | Sample source:iMicroglia	RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_035; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_035; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_035
SUBJECT_SAMPLE_FACTORS           	dKO A4 1 PBS	HSA-000028148	Genotype:GPR34/TREM2-KO | Treatment:PBS | Sample source:iMicroglia	RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_036; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_036; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_036
SUBJECT_SAMPLE_FACTORS           	dKO A4 3 PBS	HSA-000028172	Genotype:GPR34/TREM2-KO | Treatment:PBS | Sample source:iMicroglia	RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_038; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_038; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_038
SUBJECT_SAMPLE_FACTORS           	dKO A4 2 PBS	HSA-000028160	Genotype:GPR34/TREM2-KO | Treatment:PBS | Sample source:iMicroglia	RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_041; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_041; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_041
SUBJECT_SAMPLE_FACTORS           	TREM2KO1 PBS	HSA-000028145	Genotype:TREM2-KO | Treatment:PBS | Sample source:iMicroglia	RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_043; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_043; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_043
SUBJECT_SAMPLE_FACTORS           	WT4 Myelin	HSA-000028176	Genotype:WT | Treatment:Myelin | Sample source:iMicroglia	RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_045; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_045; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_045
SUBJECT_SAMPLE_FACTORS           	WT1 PBS	HSA-000028139	Genotype:WT | Treatment:PBS | Sample source:iMicroglia	RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_046; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_046; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_046
SUBJECT_SAMPLE_FACTORS           	WT4 PBS	HSA-000028175	Genotype:WT | Treatment:PBS | Sample source:iMicroglia	RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_047; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_047; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_047
SUBJECT_SAMPLE_FACTORS           	GPR34KO2 PBS	HSA-000028154	Genotype:GPR34-KO | Treatment:PBS | Sample source:iMicroglia	RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_049; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_049; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_049
SUBJECT_SAMPLE_FACTORS           	TREM2KO4 PBS	HSA-000028181	Genotype:TREM2-KO | Treatment:PBS | Sample source:iMicroglia	RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_050; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_050; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_050
SUBJECT_SAMPLE_FACTORS           	GPR34KO2 Myelin	HSA-000028155	Genotype:GPR34-KO | Treatment:Myelin | Sample source:iMicroglia	RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_051; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_051; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_051
SUBJECT_SAMPLE_FACTORS           	Pool_4	HSA-000028403	Genotype:NA | Treatment:NA | Sample source:iMicroglia	RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_052; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_052; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_052
#COLLECTION
CO:COLLECTION_SUMMARY            	iPSC1-derived microglia were harvested by aspirating medium then briefly washed
CO:COLLECTION_SUMMARY            	with 1mL of ice-cold PBS solution and directly processed for lipidomic and
CO:COLLECTION_SUMMARY            	metabolomic analysis.
CO:SAMPLE_TYPE                   	IPSC1-derived microglia
CO:STORAGE_CONDITIONS            	-80℃
CO:COLLECTION_VIALS              	Lobind 1.5 mL Eppendorf tubes
CO:STORAGE_VIALS                 	Lobind 1.5 mL Eppendorf tubes
#TREATMENT
TR:TREATMENT_SUMMARY             	Demyelination is a key feature of neuronal degeneration in vivo in AD and
TR:TREATMENT_SUMMARY             	amyloid mouse models (see Nugent et al. 2020,
TR:TREATMENT_SUMMARY             	https://doi.org/10.1016/j.neuron.2019.12.007). For this reason, we selected it
TR:TREATMENT_SUMMARY             	as a stimulus to evaluate microglial metabolism under a physiologically relevant
TR:TREATMENT_SUMMARY             	stressor in vitro. Cells were treated for 48 hours with PBS or Myelin, followed
TR:TREATMENT_SUMMARY             	by lipid extraction.
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	Cells were harvested by aspirating the medium, briefly washed with 1 mL of
SP:SAMPLEPREP_SUMMARY            	ice-cold PBS, and then lysed in 50 µL methanol containing stable-isotope
SP:SAMPLEPREP_SUMMARY            	internal standards. Samples were vortexed, adjusted to 100 µl with MS-grade
SP:SAMPLEPREP_SUMMARY            	H₂O, and transferred to 1.5 ml Protein LoBind tubes on ice. 100 µl of Methyl
SP:SAMPLEPREP_SUMMARY            	tert-butyl ether (MTBE)was added, vortexed and centrifuged at 21,000 g for 10
SP:SAMPLEPREP_SUMMARY            	min at 4 °C. The two phases were separated: the top (non-polar lipids) and
SP:SAMPLEPREP_SUMMARY            	bottom (polar metabolites) were collected into glass vials, dried overnight in a
SP:SAMPLEPREP_SUMMARY            	Genevac EZ3, and resuspended in 100 µl of methanol (lipids) or 9:1
SP:SAMPLEPREP_SUMMARY            	methanol:H₂O (metabolites) for LC-MS/MS analysis.
SP:PROCESSING_STORAGE_CONDITIONS 	On ice
SP:EXTRACT_STORAGE               	-20℃
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_TYPE           	Reversed phase
CH:INSTRUMENT_NAME               	Agilent 1290 Infinity II
CH:COLUMN_NAME                   	Waters ACQUITY UPLC BEH C18 (100 x 2.1mm,1.7um)
CH:SOLVENT_A                     	60% acetonitrile/40% water; 10 mM ammonium acetate; 0.1% acetic acid
CH:SOLVENT_B                     	90% isopropyl alcohol/10% acetonitrile; 10 mM ammonium acetate; 0.1% acetic acid
CH:FLOW_GRADIENT                 	0.0-8.0 min from 45% B to 99% B, 8.0-9.0 min at 99% B, 9.0-9.1 min to 45% B, and
CH:FLOW_GRADIENT                 	9.1-10.0 min at 45% B.
CH:FLOW_RATE                     	0.25 mL/min
CH:COLUMN_TEMPERATURE            	55
CH:SAMPLE_INJECTION              	5
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
#MS
MS:INSTRUMENT_NAME               	ABI Sciex 6500+ QTrap
MS:INSTRUMENT_TYPE               	Triple quadrupole
MS:MS_TYPE                       	ESI
MS:ION_MODE                      	NEGATIVE
MS:MS_COMMENTS                   	curtain gas at 30 psi; collision gas set at medium; ion spray voltage at 4,500
MS:MS_COMMENTS                   	V; temperature at 600°C; ion source Gas 1 at 55 psi; ion source Gas 2 at 60
MS:MS_COMMENTS                   	psi.
#MS_METABOLITE_DATA
MS_METABOLITE_DATA:UNITS	normalized peak area
MS_METABOLITE_DATA_START
Samples	HSA-000028139	HSA-000028140	HSA-000028142	HSA-000028143	HSA-000028145	HSA-000028146	HSA-000028148	HSA-000028149	HSA-000028151	HSA-000028152	HSA-000028154	HSA-000028155	HSA-000028157	HSA-000028158	HSA-000028160	HSA-000028161	HSA-000028163	HSA-000028164	HSA-000028166	HSA-000028167	HSA-000028169	HSA-000028170	HSA-000028172	HSA-000028173	HSA-000028175	HSA-000028176	HSA-000028178	HSA-000028179	HSA-000028181	HSA-000028182	HSA-000028184	HSA-000028185	HSA-000028400	HSA-000028401	HSA-000028402	HSA-000028403
Factors	Genotype:WT | Treatment:PBS | Sample source:iMicroglia	Genotype:WT | Treatment:Myelin | Sample source:iMicroglia	Genotype:GPR34-KO | Treatment:PBS | Sample source:iMicroglia	Genotype:GPR34-KO | Treatment:Myelin | Sample source:iMicroglia	Genotype:TREM2-KO | Treatment:PBS | Sample source:iMicroglia	Genotype:TREM2-KO | Treatment:Myelin | Sample source:iMicroglia	Genotype:GPR34/TREM2-KO | Treatment:PBS | Sample source:iMicroglia	Genotype:GPR34/TREM2-KO | Treatment:Myelin | Sample source:iMicroglia	Genotype:WT | Treatment:PBS | Sample source:iMicroglia	Genotype:WT | Treatment:Myelin | Sample source:iMicroglia	Genotype:GPR34-KO | Treatment:PBS | Sample source:iMicroglia	Genotype:GPR34-KO | Treatment:Myelin | Sample source:iMicroglia	Genotype:TREM2-KO | Treatment:PBS | Sample source:iMicroglia	Genotype:TREM2-KO | Treatment:Myelin | Sample source:iMicroglia	Genotype:GPR34/TREM2-KO | Treatment:PBS | Sample source:iMicroglia	Genotype:GPR34/TREM2-KO | Treatment:Myelin | Sample source:iMicroglia	Genotype:WT | Treatment:PBS | Sample source:iMicroglia	Genotype:WT | Treatment:Myelin | Sample source:iMicroglia	Genotype:GPR34 KO | Treatment:PBS | Sample source:iMicroglia	Genotype:GPR34-KO | Treatment:Myelin | Sample source:iMicroglia	Genotype:TREM2-KO | Treatment:PBS | Sample source:iMicroglia	Genotype:TREM2-KO | Treatment:Myelin | Sample source:iMicroglia	Genotype:GPR34/TREM2-KO | Treatment:PBS | Sample source:iMicroglia	Genotype:GPR34/TREM2-KO | Treatment:Myelin | Sample source:iMicroglia	Genotype:WT | Treatment:PBS | Sample source:iMicroglia	Genotype:WT | Treatment:Myelin | Sample source:iMicroglia	Genotype:GPR34-KO | Treatment:PBS | Sample source:iMicroglia	Genotype:GPR34-KO | Treatment:Myelin | Sample source:iMicroglia	Genotype:TREM2-KO | Treatment:PBS | Sample source:iMicroglia	Genotype:TREM2-KO | Treatment:Myelin | Sample source:iMicroglia	Genotype:GPR34/TREM2-KO | Treatment:PBS | Sample source:iMicroglia	Genotype:GPR34/TREM2-KO | Treatment:Myelin | Sample source:iMicroglia	Genotype:NA | Treatment:NA | Sample source:iMicroglia	Genotype:NA | Treatment:NA | Sample source:iMicroglia	Genotype:NA | Treatment:NA | Sample source:iMicroglia	Genotype:NA | Treatment:NA | Sample source:iMicroglia
(3-O-sulfo)GalCer(d18:1/16:0)	N.D.	0.101	N.D.	0.087	N.D.	0.082	N.D.	0.107	N.D.	0.119	N.D.	0.096	N.D.	0.107	N.D.	0.116	N.D.	0.082	N.D.	0.093	N.D.	0.09	N.D.	0.089	N.D.	0.121	N.D.	0.112	N.D.	0.122	N.D.	0.117	0.083	0.053	0.068	0.072
(3-O-sulfo)GalCer(d18:1/18:0(2OH)	N.D.	1.036	N.D.	0.969	N.D.	0.947	N.D.	0.984	N.D.	1.112	N.D.	1.028	N.D.	1.189	N.D.	1.124	N.D.	1.038	N.D.	0.994	N.D.	0.97	N.D.	0.964	N.D.	1.174	N.D.	1.13	N.D.	1.038	N.D.	1.046	0.724	0.778	0.757	0.817
(3-O-sulfo)GalCer(d18:1/18:0(d3))	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
(3-O-sulfo)GalCer(d18:1/18:0)	N.D.	4.76	N.D.	4.737	N.D.	4.145	N.D.	5.145	N.D.	5.177	N.D.	5.206	N.D.	4.575	N.D.	4.769	N.D.	5.273	N.D.	5.211	N.D.	4.588	N.D.	4.605	N.D.	5.247	N.D.	5.2	N.D.	4.668	N.D.	5.752	4.073	4.102	3.803	3.956
(3-O-sulfo)GalCer(d18:1/24:0(2OH))	N.D.	10.917	N.D.	10.818	N.D.	12.233	N.D.	11.984	N.D.	11.433	N.D.	10.542	N.D.	13.32	N.D.	11.679	N.D.	10.653	N.D.	10.829	N.D.	12.458	N.D.	11.554	N.D.	10.811	N.D.	11.484	N.D.	11.847	N.D.	13.562	9.786	9.45	9.171	9.739
(3-O-sulfo)GalCer(d18:1/24:0)	0.012	23.216	0.018	23.888	0.004	26.356	0.007	26.7	0.015	23.007	0.003	24.58	0.014	27.197	0.003	25.506	0.018	26.14	0.022	25.012	0.025	26.752	0.01	26.1	0.007	22.805	0.017	27.297	0.012	22.882	0.024	30.568	20.562	21.34	19.605	21.412
(3-O-sulfo)GalCer(d18:1/24:1(2OH))	N.D.	10.414	N.D.	9.667	N.D.	9.663	N.D.	10.554	N.D.	9.992	N.D.	9.819	N.D.	9.554	N.D.	10.628	N.D.	10.246	N.D.	8.439	N.D.	10.286	N.D.	9.9	N.D.	9.457	N.D.	10.797	N.D.	9.63	N.D.	12.274	9.23	9.598	9.396	9.247
(3-O-sulfo)GalCer(d18:1/24:1)	0.047	46.066	0.04	47.056	0.019	48.303	0.032	47.729	0.032	46.479	0.024	44.299	0.041	45.136	0.055	52.59	0.025	44.482	0.043	44.914	0.043	46.577	0.055	48.913	0.017	42.369	0.057	47.64	0.024	45.677	0.053	56.122	48.692	50.472	51.259	49.653
Arachidonic acid	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Arachidonic acid-d8	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Arachidonic acid-d8_MRM	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Arachidonic acid_MRM	0.048	0.09	0.223	0.234	0.061	0.1	0.203	0.205	0.063	0.098	0.255	0.164	0.124	0.078	0.084	0.085	0.116	0.092	0.349	0.342	0.35	0.364	0.215	0.226	0.186	0.213	0.231	0.153	0.186	0.176	0.103	0.14	0.181	0.165	0.173	0.169
BMP(14:0/14:0)	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
BMP(16:0_18:1)	0.074	0.169	0.077	0.118	0.054	0.212	0.139	0.223	0.072	0.243	0.165	0.263	0.12	0.389	0.04	0.082	0.071	0.191	0.255	0.336	0.175	0.371	0.171	0.243	0.091	0.148	0.129	0.251	0.271	0.505	0.212	0.381	0.178	0.194	0.192	0.196
BMP(16:0_20:4)	0.014	0.014	0.028	0.025	0.02	0.041	0.029	0.029	0.01	0.021	0.035	0.042	0.057	0.054	0.018	0.023	0.01	0.016	0.058	0.055	0.077	0.069	0.052	0.051	0.022	0.025	0.042	0.036	0.083	0.07	0.048	0.048	0.038	0.04	0.033	0.036
BMP(16:0_22:6)	0.001	0.005	0.001	0.002	0	0.005	0.003	0.004	0.001	0.004	0.005	0.01	0.001	0.01	0.002	0.002	0.002	0.004	0.005	0.01	0.002	0.008	0.003	0.005	0.001	0.003	0.003	0.006	0.004	0.008	0.003	0.008	0.005	0.004	0.004	0.004
BMP(16:1/16:1)	0.006	0.007	0.002	0.004	0.001	0.003	0.003	0.006	0.009	0.009	0.005	0.005	0.002	0.004	0.001	0	0.007	0.007	0.006	0.006	0.002	0.003	0.002	0.002	0.003	0.002	0.001	0.003	0.002	0.003	0.002	0.004	0.004	0.004	0.003	0.003
BMP(18:0_18:1)	0.096	0.205	0.056	0.11	0.036	0.184	0.14	0.234	0.088	0.368	0.25	0.449	0.164	0.4	0.033	0.06	0.101	0.289	0.388	0.574	0.23	0.372	0.218	0.307	0.114	0.159	0.157	0.307	0.381	0.699	0.308	0.516	0.288	0.27	0.272	0.272
BMP(18:0_20:4)	0.008	0.01	0.013	0.017	0.013	0.023	0.02	0.028	0.009	0.016	0.027	0.038	0.039	0.035	0.01	0.015	0.007	0.014	0.049	0.047	0.054	0.049	0.04	0.036	0.018	0.016	0.033	0.034	0.087	0.053	0.042	0.05	0.031	0.03	0.03	0.031
BMP(18:0_22:6)	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
BMP(18:1/18:1)	1.529	2.485	0.986	1.696	0.617	2.378	1.837	3.018	1.791	3.814	2.703	4.413	1.466	4.406	0.615	1.055	1.613	3.298	3.828	5.729	2.204	4.903	2.439	3.585	1.708	2.356	2.133	3.961	3.521	5.975	2.845	5.93	2.827	2.556	2.687	2.82
BMP(20:4/20:4)	0.039	0.024	0.117	0.074	0.102	0.091	0.106	0.084	0.029	0.028	0.103	0.091	0.301	0.118	0.088	0.106	0.028	0.032	0.182	0.141	0.371	0.196	0.206	0.156	0.084	0.077	0.201	0.1	0.42	0.172	0.239	0.151	0.157	0.144	0.159	0.146
BMP(22:6/22:6)	0.045	0.164	0.066	0.211	0.023	0.225	0.131	0.336	0.054	0.214	0.202	0.525	0.098	0.354	0.023	0.077	0.037	0.189	0.238	0.453	0.075	0.212	0.103	0.25	0.069	0.15	0.133	0.396	0.142	0.31	0.153	0.32	0.179	0.153	0.157	0.168
CL(14:0/14:0/14:0/14:0)	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
CL(72:6/18:2)	2.134	1.66	1.073	1.227	0.554	1.329	2.479	2.059	2.242	2.455	2.453	2.616	1.629	1.631	0.666	0.612	1.962	2.54	3.407	2.446	2.087	1.951	1.976	1.858	2.589	1.792	1.748	1.995	3.679	2.037	2.4	2.453	1.807	1.776	2.017	1.823
CL(72:7/18:2)	2.972	2.644	1.671	1.66	0.884	1.871	4.401	3.135	4.14	3.313	4.102	4.837	2.624	2.256	1.267	0.901	3.298	3.302	5.276	4.439	2.877	3.266	3.631	3.131	3.593	2.172	3.501	3.157	5.13	3.41	3.226	3.697	3.25	2.87	3.238	3.586
CL(72:8-2(OOH)/18:2)	0.252	0.373	0.212	0.426	0.059	0.224	0.188	0.41	0.383	0.572	0.414	0.714	0.104	0.222	0.076	0.177	0.293	0.625	0.65	0.67	0.097	0.397	0.159	0.432	0.186	0.442	0.195	0.516	0.279	0.386	0.193	0.53	0.32	0.293	0.413	0.445
CL(72:8/18:2)	2.006	1.729	1.458	1.313	0.545	1.197	3.047	1.987	3.119	1.973	4.618	3.86	1.316	1.535	0.714	0.568	2.705	2.483	4.877	3.454	2.214	2.076	2.722	2.635	2.661	1.616	2.05	2.091	3.203	2.263	2.464	2.611	2.131	2.328	2.262	2.055
CL(74:9/18:2)	1.068	0.801	0.685	0.829	0.393	0.828	2.047	1.431	1.325	1.197	2.365	2.01	0.979	1.194	0.5	0.429	0.805	1.242	3.206	2.468	1.413	1.249	1.931	1.743	1.189	0.719	1.402	1.511	2.375	1.465	1.734	2.169	1.355	1.348	1.326	1.415
Cholesterol sulfate	1.808	0.37	1.527	0.443	1.444	0.37	1.436	0.418	1.925	0.371	1.65	0.367	1.202	0.412	1.578	0.455	1.752	0.391	1.728	0.406	1.145	0.373	1.48	0.419	1.686	0.346	1.438	0.443	1.025	0.369	1.101	0.425	0.805	0.707	0.715	0.713
DHA	0.007	0.01	0.02	0.019	0.005	0.012	0.019	0.022	0.005	0.006	0.022	0.016	0.013	0.011	0.009	0.011	0.01	0.008	0.026	0.02	0.018	0.022	0.017	0.016	0.009	0.013	0.016	0.012	0.016	0.014	0.013	0.014	0.012	0.014	0.014	0.013
EPA	0.015	0.023	0.053	0.054	0.015	0.026	0.062	0.061	0.024	0.039	0.096	0.086	0.036	0.023	0.02	0.017	0.021	0.017	0.127	0.112	0.098	0.095	0.063	0.057	0.069	0.051	0.106	0.059	0.082	0.063	0.054	0.047	0.051	0.056	0.055	0.054
FAHFA(18:1/9-O-18:0)	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
GD1a/b(d36:1)	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
GD3(d34:1)	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
GD3(d36:1)	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
GM3(d18:1/18:0(d5))	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
GM3(d34:1)	2.353	2.71	2.23	2.754	1.849	2.355	3.129	3.731	2.813	2.967	3.458	4.755	2.364	2.988	1.131	1.433	2.323	2.57	5.319	4.534	2.42	3.477	2.735	3.176	2.675	2.246	2.999	4.099	4.162	4.606	3.08	3.343	3.033	3.954	4.529	3.798
GM3(d36:1)	0.815	0.78	0.911	1.028	0.726	0.92	1.305	1.762	0.949	0.926	1.772	1.999	0.847	1.265	0.367	0.677	0.74	0.881	2.163	2.373	0.955	1.372	1.471	1.843	0.745	0.924	1.222	1.687	1.591	2.025	1.53	1.832	1.228	1.511	1.489	1.4
GQ1b(d36:1)	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Hemi-BMP(14:0/14:0)_14:0	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Hemi-BMP(18:1/18:1)_16:0	0.001	0.008	0.002	0.008	0.001	0.008	0.004	0.007	0.002	0.014	0.009	0.019	0.002	0.02	0.001	0.002	0.002	0.012	0.011	0.024	0.004	0.014	0.003	0.008	0.002	0.01	0.007	0.017	0.004	0.025	0.005	0.014	0.006	0.008	0.006	0.006
Hemi-BMP(18:1/18:1)_18:0	0.001	0.003	0.002	0.002	0.001	0.004	0.002	0.002	0.001	0.004	0.002	0.005	0.001	0.006	0.001	0.001	0.001	0.003	0.003	0.005	0.003	0.005	0.002	0.003	0.001	0.002	0.003	0.005	0.002	0.009	0.002	0.005	0.002	0.003	0.002	0.002
Hemi-BMP(18:1/18:1)_18:1	0.002	0.006	0.002	0.006	0.002	0.006	0.004	0.005	0.002	0.01	0.006	0.013	0.002	0.011	0.001	0.002	0.002	0.007	0.007	0.016	0.003	0.009	0.003	0.006	0.002	0.007	0.006	0.011	0.004	0.017	0.004	0.011	0.005	0.004	0.005	0.005
Hemi-BMP(20:4/20:4)_16:0	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Hemi-BMP(20:4/20:4)_18:0	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Hemi-BMP(20:4/20:4)_18:1	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Hemi-BMP(20:4/20:4)_20:4	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Hemi-BMP(22:6/22:6)_16:0	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Hemi-BMP(22:6/22:6)_18:0	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Hemi-BMP(22:6/22:6)_18:1	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Hemi-BMP(22:6/22:6)_22:6	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
LPE(16:0)	0.048	0.225	0.096	0.16	0.056	0.167	0.083	0.161	0.054	0.181	0.076	0.178	0.045	0.125	0.042	0.136	0.029	0.144	0.077	0.117	0.048	0.129	0.053	0.126	0.027	0.257	0.06	0.154	0.064	0.138	0.061	0.177	0.097	0.103	0.101	0.106
LPE(18:0)	0.625	6.007	0.985	3.257	0.865	3.039	0.853	2.794	0.555	5.944	1.162	4.059	0.844	3.571	0.533	2.003	0.552	5.765	1.381	3.544	0.817	3.243	1.048	2.866	0.439	5.745	0.94	3.3	0.927	3.015	0.979	2.937	1.87	1.849	1.83	1.879
LPE(18:1(d7))	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
LPG(16:0)	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
LPG(18:0)	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
LPG(18:1)	0.016	0.027	0.021	0.025	0.013	0.025	0.025	0.022	0.03	0.051	0.048	0.067	0.032	0.047	0.012	0.014	0.029	0.027	0.058	0.067	0.041	0.028	0.011	0.015	0.038	0.048	0.048	0.062	0.066	0.062	0.042	0.028	0.036	0.034	0.038	0.038
LPG(20:4)	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
LPG(22:6)	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
LPI(16:0)	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
LPI(18:0)	0.017	0.042	0.029	0.063	0.018	0.055	0.035	0.051	0.033	0.085	0.094	0.194	0.046	0.124	0.011	0.028	0.032	0.054	0.109	0.15	0.032	0.049	0.01	0.02	0.054	0.127	0.116	0.206	0.073	0.152	0.061	0.059	0.062	0.063	0.056	0.059
Linoleic acid	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Linolenic acid	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Oleic acid	0.417	0.571	0.409	0.501	0.197	0.367	0.472	0.556	0.404	0.809	0.52	0.709	0.415	0.284	0.282	0.247	0.362	0.492	0.775	0.78	0.541	1.071	0.335	0.651	0.599	0.64	0.454	0.661	0.585	0.443	0.253	0.293	0.477	0.496	0.478	0.514
PA(15:0/18:1(d7))	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
PA(16:0_18:1)	0.57	2.502	0.257	4.129	0.116	2.325	0.349	3.64	0.464	3.555	0.69	3.571	0.289	1.561	0.089	4.882	0.447	3.352	1.055	3.519	0.245	2.765	0.231	4.106	0.401	5.198	0.559	5.419	0.413	3.026	0.339	4.973	1.64	1.444	1.458	1.532
PA(18:0_18:1)	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
PA(18:0_20:4)	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
PA(18:0_22:6)	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
PA(18:1/18:1)	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
PE(15:0/18:1(d7))	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
PE(O-16:0/20:4)	2.475	2.625	1.899	2.524	0.9	1.635	3.07	3.135	2.278	2.943	4.254	4.152	1.805	2.294	0.862	1.34	2.053	2.872	5.104	4.306	2.097	2.211	2.633	2.604	2.317	2.479	2.976	3.507	2.735	2.72	3.024	3.455	2.818	2.626	2.746	2.977
PE(O-16:0/22:6)	0.448	0.417	0.25	0.351	0.06	0.162	0.375	0.352	0.467	0.512	0.622	0.645	0.135	0.213	0.068	0.14	0.398	0.499	0.745	0.581	0.147	0.241	0.222	0.262	0.382	0.397	0.37	0.425	0.226	0.284	0.264	0.385	0.334	0.356	0.396	0.436
PE(O-18:0/20:4)	1.276	3.309	1.012	2.759	0.499	2.474	1.514	3.385	1.198	4.166	2.527	5.428	1.176	3.585	0.445	2.107	1.058	4.124	3.55	6.013	1.499	2.887	1.522	3.349	1.389	3.559	2.037	4.899	2.005	4.122	2.024	4.703	2.451	2.165	2.413	2.433
PE(O-18:0/22:6)	0.148	0.349	0.078	0.225	0.027	0.14	0.122	0.235	0.155	0.426	0.227	0.457	0.059	0.148	0.015	0.17	0.151	0.426	0.29	0.474	0.072	0.157	0.075	0.21	0.166	0.345	0.127	0.311	0.103	0.24	0.113	0.321	0.191	0.174	0.191	0.173
PE(P-16:0/20:4)	28.092	29.078	25.79	28.419	13.973	20.712	35.791	36.476	28.839	33.514	50.729	52.71	27.458	29.535	14.748	15.778	23.464	32.204	59.851	55.709	32.76	31.43	33.334	34.958	25.182	24.245	38.32	42	39.658	32.029	41.131	44.988	35.937	33.294	33.03	38.055
PE(P-16:0/20:5)	3.546	4.666	2.523	5.969	0.98	3.132	6.878	10.176	4.212	5.974	11.444	13.67	3.151	4.346	1.013	2.095	2.842	4.696	13.292	14.993	4.063	5.63	5.243	8.368	2.695	3.791	6.568	11.252	7.771	9.849	7.839	13.747	6.6	7.02	7.02	7.698
PE(P-16:0/22:4)	13.959	25.008	10.702	22.401	4.489	16.974	12.351	23.921	17.217	29.833	22.54	40.336	8.746	21.78	4.243	19.124	12.902	27.3	29.411	40.23	11.96	22.202	10.887	24.362	13.865	27.528	15.371	33.597	15.61	27.431	14.395	31.052	19.611	18.58	20.296	21.038
PE(P-16:0/22:6)	2.567	4.031	1.505	3.442	0.411	1.788	2.206	3.033	3.899	5.572	4.509	6.615	1.002	2.282	0.39	1.447	2.864	5.718	5.273	6.883	1.159	2.275	1.46	2.897	2.653	4.2	2.449	4.963	1.964	3.278	2.099	3.874	3.076	2.906	3.208	3.204
PE(P-18:0/18:1(d9))	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
PE(P-18:0/18:1)	2.531	133.431	1.694	150.002	1.09	132.321	2.433	156.576	3.538	149	4.717	159.124	2.307	118.561	0.865	182.228	2.985	137.06	6.198	126.299	2.969	129.326	2.452	150.536	2.73	215.16	3.082	201.837	4.204	160.072	3.669	196.168	49.762	49.949	55.919	50.328
PE(P-18:0/18:2)	0.549	2.943	0.339	2.554	0.145	2.037	0.426	2.664	0.923	3.781	0.914	3.563	0.32	2.026	0.131	2.493	0.723	3.991	1.118	3.16	0.419	2.377	0.382	2.779	0.61	3.662	0.566	3.808	0.532	3.285	0.524	3.628	1.336	1.225	1.377	1.363
PE(P-18:0/20:4)	12.178	30.893	9.742	29.36	5.576	24.679	14.424	30.43	14.135	37.571	24.418	55.917	14.257	36.393	6.257	22.763	11.603	37.02	31.942	54.306	17.244	31.002	16.13	36.557	13.158	33.612	19.361	49.256	20.73	43.833	21.544	46.267	24.109	22.928	27.169	25.855
PE(P-18:0/20:5)	1.059	2.978	0.99	2.693	0.399	1.902	1.897	4.604	1.432	3.956	2.961	7.026	1.331	3.252	0.407	1.263	0.932	3.529	4.613	8.219	1.608	2.835	1.892	4.047	1.059	2.042	2.226	6.046	2.613	4.745	2.925	6.175	2.866	2.564	2.785	2.887
PE(P-18:0/22:6)	0.974	3.023	0.423	2.253	0.163	1.545	0.602	2.442	1.315	4.041	1.302	4.202	0.354	1.636	0.129	2.215	1.159	3.898	1.7	3.851	0.459	1.723	0.521	2.428	1.046	3.736	0.789	3.393	0.597	2.349	0.728	3.352	1.565	1.384	1.503	1.558
PE(P-18:1/20:4)	29.114	43.752	23.057	40.112	10.051	31.935	29.093	49.692	32.344	52.465	45.593	72.14	19.918	50.125	11.615	26.324	24.575	50.414	50.638	70.644	24.61	40.723	24.081	42.177	24.843	39.964	28.364	60.68	29.02	44.501	30.089	52.431	39.597	36.415	36.859	38.991
PE(P-18:1/22:6)	2.439	5.752	1.383	4.322	0.301	2.178	1.721	3.754	3.637	7.446	4.018	7.977	0.781	3.167	0.305	1.97	3.064	6.856	4.287	7.765	0.825	2.962	1.083	3.124	2.429	5.457	2.033	5.704	1.281	4.038	1.547	4.38	3.423	2.813	3.259	3.226
PEth(16:0_18:1)	0.078	0.13	0.043	0.107	0.015	0.067	0.05	0.09	0.086	0.169	0.098	0.184	0.026	0.088	0.014	0.064	0.073	0.179	0.097	0.165	0.026	0.077	0.037	0.088	0.068	0.123	0.063	0.143	0.036	0.077	0.038	0.095	0.083	0.081	0.079	0.074
PEth(18:1/18:1)	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
PG(15:0/18:1(d7))	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
PG(16:0_18:1)	0.238	0.271	0.265	0.317	0.093	0.214	0.305	0.405	0.195	0.456	0.43	0.575	0.248	0.378	0.093	0.164	0.204	0.314	0.508	0.67	0.282	0.353	0.339	0.387	0.23	0.234	0.341	0.495	0.369	0.345	0.359	0.477	0.357	0.332	0.347	0.334
PG(16:0_20:4)	0.002	0.003	0.003	0.004	0.002	0.004	0.003	0.005	0.002	0.005	0.002	0.006	0.002	0.008	0.001	0.002	0.001	0.004	0.006	0.01	0.004	0.006	0.005	0.005	0.003	0.003	0.004	0.006	0.004	0.008	0.004	0.007	0.004	0.005	0.005	0.003
PG(16:0_22:6)	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
PG(18:0_18:1)	0.121	0.136	0.096	0.086	0.039	0.07	0.087	0.111	0.071	0.219	0.098	0.163	0.075	0.124	0.03	0.046	0.096	0.173	0.119	0.162	0.093	0.113	0.093	0.105	0.138	0.132	0.104	0.127	0.115	0.121	0.1	0.128	0.11	0.111	0.119	0.103
PG(18:0_20:4)	0.004	0.005	0.003	0.005	0.001	0.004	0.004	0.008	0.003	0.009	0.005	0.008	0.002	0.008	0	0.004	0.003	0.005	0.006	0.011	0.003	0.009	0.004	0.008	0.003	0.005	0.004	0.009	0.007	0.01	0.006	0.013	0.006	0.006	0.005	0.004
PG(18:0_22:6)	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
PG(18:1/18:1)	0.073	0.078	0.058	0.071	0.03	0.047	0.066	0.098	0.062	0.229	0.093	0.126	0.045	0.079	0.017	0.035	0.061	0.123	0.104	0.146	0.056	0.074	0.069	0.088	0.065	0.092	0.082	0.109	0.092	0.109	0.087	0.101	0.083	0.082	0.077	0.085
PI(15:0/18:1(d7))	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
PI(16:0_18:1)	0.26	1.067	0.167	0.775	0.078	0.845	0.205	0.827	0.267	1.159	0.284	0.961	0.17	1.027	0.068	0.614	0.35	1.034	0.322	0.89	0.205	0.952	0.163	0.772	0.268	1.188	0.201	0.897	0.214	0.88	0.153	0.854	0.499	0.415	0.456	0.431
PI(16:0_20:4)	0.812	1.814	0.84	1.621	0.436	1.593	1.274	1.99	0.811	2.082	1.635	2.212	1.003	1.732	0.531	1.261	0.718	1.726	2.202	2.491	1.146	1.859	1.223	1.772	0.719	1.727	1.123	1.881	1.487	1.665	1.151	2.002	1.376	1.193	1.389	1.278
PI(16:0_22:6)	0.038	0.225	0.031	0.214	0.017	0.187	0.042	0.238	0.042	0.242	0.076	0.212	0.046	0.206	0.014	0.202	0.04	0.222	0.091	0.204	0.027	0.183	0.042	0.228	0.03	0.248	0.04	0.223	0.041	0.185	0.044	0.241	0.101	0.101	0.1	0.085
PI(18:0_18:1)	0.622	2.315	0.359	1.555	0.146	1.657	0.317	1.58	0.798	2.857	0.57	2.541	0.286	2.41	0.096	0.947	0.883	3.237	0.726	2.505	0.389	2.193	0.343	1.582	0.747	2.553	0.495	2.128	0.384	2.271	0.291	1.871	1.068	0.937	0.994	0.987
PI(18:0_20:4)	19.147	27.055	16.979	24.597	7.335	18.882	20.465	32.738	18.786	28.889	31.545	39.712	18.146	22.745	7.753	11.972	20.697	29.66	39.089	45.677	20.198	25.493	23.739	30.559	17.144	21.918	24.498	31.929	24.298	26.075	23.185	31.856	24.504	21.129	24.069	23.167
PI(18:0_22:6)	0.288	0.671	0.205	0.536	0.096	0.528	0.3	0.652	0.361	0.834	0.521	0.883	0.23	0.679	0.09	0.372	0.355	0.79	0.626	0.88	0.276	0.605	0.301	0.69	0.305	0.647	0.311	0.683	0.369	0.637	0.289	0.728	0.495	0.439	0.428	0.449
PI(18:1/18:1)	2.277	4.002	1.026	1.963	0.351	1.511	0.99	2.119	3.021	4.94	1.711	3.066	0.702	1.879	0.317	0.932	3.175	4.752	2.031	3.182	0.807	1.895	0.84	1.843	2.301	3.375	1.394	2.28	0.953	1.984	0.923	1.834	1.874	1.679	1.916	1.857
PI(20:4/20:4)	0.007	0.017	0.018	0.017	0.005	0.022	0.012	0.02	0.006	0.021	0.031	0.025	0.023	0.017	0.004	0.01	0.011	0.017	0.036	0.033	0.029	0.025	0.021	0.023	0.016	0.016	0.02	0.026	0.036	0.023	0.016	0.019	0.022	0.017	0.015	0.022
PS(15:0/18:1(d7))	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
PS(16:0_18:1)	2.805	3.93	1.431	3.238	0.573	1.703	1.493	2.123	3.708	3.963	3.247	4.082	1.032	1.437	0.448	1.656	3.615	4.383	4.452	4.695	1.065	1.894	1.125	2.262	2.933	3.762	2.03	4.059	1.498	2.389	1.406	3.076	2.546	2.291	2.079	1.687
PS(16:0_20:4)	0.191	0.194	0.2	0.325	0.087	0.157	0.252	0.279	0.183	0.207	0.339	0.334	0.182	0.161	0.112	0.176	0.199	0.163	0.435	0.419	0.166	0.221	0.208	0.271	0.157	0.224	0.25	0.264	0.178	0.21	0.179	0.278	0.265	0.219	0.242	0.216
PS(16:0_22:6)	0.284	0.166	0.135	0.203	0.047	0.087	0.228	0.184	0.245	0.15	0.375	0.317	0.113	0.087	0.039	0.1	0.236	0.158	0.338	0.404	0.129	0.135	0.145	0.159	0.163	0.155	0.175	0.189	0.133	0.186	0.168	0.214	0.231	0.209	0.177	0.169
PS(18:0_18:1)	69.314	168.553	46.171	160.503	13.836	143.533	71.502	144.129	83.356	174.159	122.935	197.918	53.159	148.056	15.002	134.002	95.802	209.158	165.573	237.607	63.848	169.262	62.926	188.159	69.296	168.386	92.759	209.443	74.636	169.246	85.078	230.168	137.956	94.956	93.075	108.663
PS(18:0_20:4)	6.406	12.645	5.62	13.908	1.94	6.277	5.93	9.045	6.2	11.171	13.333	20.028	4.414	6.398	2.421	6.016	6.009	14.169	18.391	24.648	4.457	7.634	6.669	9.005	7.968	12.959	12.105	17.85	5.756	11.417	8.522	12.026	9.318	9.493	8.631	6.726
PS(18:0_22:6)	11.873	10.548	5.349	8.711	1.921	4.921	7.952	9.188	13.95	12.196	15.487	12.298	4.467	4.486	1.88	4.852	14.335	12.435	17.242	18.733	4.258	5.179	7.036	7.914	8.216	8.476	8.526	10.509	6.385	4.989	7.023	9.849	8.931	7.157	9.139	6.483
PS(18:1/18:1)	4.118	15.355	1.404	15.324	0.452	11.145	1.575	11.633	6.637	18.105	4.059	18.825	1.014	9.062	0.466	14.209	7.289	18.549	6.041	16.481	1.33	10.523	1.442	12.438	5.484	21.449	2.857	21.338	1.505	15.894	1.978	17.36	7.57	6.701	5.99	6.056
PS(22:6/22:6)	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Palmitic acid	3.504	2.898	2.447	2.369	2.518	2.668	2.966	3.177	3.027	3.359	2.917	3.735	3.089	2.533	3.214	2.412	2.38	2.615	3.841	3.953	3.229	4.93	2.863	3.874	3.037	2.643	2.781	3.99	3.597	2.708	2.958	2.621	3.068	3.4	3.22	3.266
Palmitoleic acid	0.061	0.104	0.092	0.108	0.033	0.056	0.076	0.096	0.125	0.104	0.12	0.088	0.039	0.03	0.04	0.04	0.088	0.083	0.126	0.118	0.075	0.079	0.053	0.107	0.113	0.1	0.076	0.062	0.045	0.044	0.033	0.043	0.079	0.086	0.081	0.087
Stearic acid	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
MS_METABOLITE_DATA_END
#METABOLITES
METABOLITES_START
metabolite_name	Expected RT	MS1_mz	MS2_mz
(3-O-sulfo)GalCer(d18:1/16:0)	4.45	778.5	97
(3-O-sulfo)GalCer(d18:1/18:0(2OH)	4.87	822.6	97
(3-O-sulfo)GalCer(d18:1/18:0(d3))	5.06	809.6	97
(3-O-sulfo)GalCer(d18:1/18:0)	4.96	806.6	97
(3-O-sulfo)GalCer(d18:1/24:0(2OH))	6.14	906.7	97
(3-O-sulfo)GalCer(d18:1/24:0)	6.19	890.7	97
(3-O-sulfo)GalCer(d18:1/24:1(2OH))	5.73	904.7	97
(3-O-sulfo)GalCer(d18:1/24:1)	5.79	888.7	97
Arachidonic acid	2.33	303.2	303.2
Arachidonic acid_MRM	2.33	303.2	259.1
Arachidonic acid-d8	2.3	311.3	311.3
Arachidonic acid-d8_MRM	2.3	311.3	267.1
BMP(14:0/14:0)	3.69	665.3	227.2
BMP(16:0_18:1)	4.8	747.5	255.4
BMP(16:0_20:4)	4.13	769.5	255.4
BMP(16:0_22:6)	4.2	795.5	255.4
BMP(16:1/16:1)	3.79	717.5	253.1
BMP(18:0_18:1)	5.25	775.5	281.4
BMP(18:0_20:4)	4.69	797.5	283.4
BMP(18:0_22:6)	4.7	823.5	283.4
BMP(18:1/18:1)	4.84	773.5	281.3
BMP(20:4/20:4)	3.69	817.5	303.3
BMP(22:6/22:6)	3.45	865.5	327.3
Cholesterol sulfate	3.08	465.3	96.7
CL(14:0/14:0/14:0/14:0)	7.17	619.5	227.2
CL(72:6/18:2)	7.48	725.7	279.2
CL(72:7/18:2)	7.24	724.7	279.2
CL(72:8-2(OOH)/18:2)	6.49	755.7	279.2
CL(72:8/18:2)	7.26	723.7	279.3
CL(74:9/18:2)	7.29	736.7	279.2
DHA	2.14	327.2	229.1
EPA	1.97	301.3	257.1
FAHFA(18:1/9-O-18:0)	6.2	563.6	281
GD1a/b(d36:1)	4.54	917.5	290.1
GD3(d34:1)	4.1	720.9	290.1
GD3(d36:1)	4.65	734.9	290.1
GM3(d18:1/18:0(d5))	5.03	1184.8	290.1
GM3(d34:1)	4.54	1151.7	290.1
GM3(d36:1)	5.05	1179.8	290.1
GQ1b(d36:1)	4.13	1208.6	290.1
Hemi-BMP(14:0/14:0)_14:0	6.2	875.5	227.3
Hemi-BMP(18:1/18:1)_16:0	7.1	1011.7	281.3
Hemi-BMP(18:1/18:1)_18:0	7.2	1039.7	281.3
Hemi-BMP(18:1/18:1)_18:1	7.1	1037.7	281.3
Hemi-BMP(20:4/20:4)_16:0	6.4	1056.8	303.3
Hemi-BMP(20:4/20:4)_18:0	6.6	1185.8	303.3
Hemi-BMP(20:4/20:4)_18:1	6.4	1183.8	303.3
Hemi-BMP(20:4/20:4)_20:4	6.2	1104.8	303.3
Hemi-BMP(22:6/22:6)_16:0	6.2	1103.7	327.3
Hemi-BMP(22:6/22:6)_18:0	6.5	1131.7	327.3
Hemi-BMP(22:6/22:6)_18:1	6.2	1129.7	327.3
Hemi-BMP(22:6/22:6)_22:6	5.6	1175.7	327.3
Linoleic acid	2.46	279.2	279.2
Linolenic acid	2.08	277.2	277.2
LPE(16:0)	1.97	452.2	255.3
LPE(18:0)	2.61	480.3	283.3
LPE(18:1(d7))	2	485.3	288.3
LPG(16:0)	1.65	483.3	255.3
LPG(18:0)	2.11	511.3	283.3
LPG(18:1)	1.64	509.3	281.3
LPG(20:4)	1.29	531.3	303.3
LPG(22:6)	1.28	555.3	327.3
LPI(16:0)	1.6	571.3	241.1
LPI(18:0)	2.12	599.3	241.1
Oleic acid	2.96	281.2	281.2
PA(15:0/18:1(d7))	5.09	666.5	241.3
PA(16:0_18:1)	5.45	673.5	255.3
PA(18:0_18:1)	5.84	701.5	283.3
PA(18:0_20:4)	5.42	723.5	283.3
PA(18:0_22:6)	5.35	747.5	283.3
PA(18:1/18:1)	5.36	699.5	281.3
Palmitic acid	2.98	255.1	255.1
Palmitoleic acid	2.35	253.1	253.1
PE(15:0/18:1(d7))	5.49	709.6	241.3
PE(O-16:0/20:4)	5.48	724.5	303.2
PE(O-16:0/22:6)	5.38	748.5	327.2
PE(O-18:0/20:4)	5.97	752.6	303.2
PE(O-18:0/22:6)	5.87	776.6	327.2
PE(P-16:0/20:4)	5.48	722.6	303.3
PE(P-16:0/20:5)	5.11	720.6	301.3
PE(P-16:0/22:4)	5.83	750.6	331.3
PE(P-16:0/22:6)	5.29	746.6	327.3
PE(P-18:0/18:1)	6.4	728.6	281.3
PE(P-18:0/18:2)	6.08	726.6	279.2
PE(P-18:0/20:4)	5.96	750.6	303.3
PE(P-18:0/20:5)	5.61	748.6	301.3
PE(P-18:0/22:6)	5.79	774.6	327.3
PE(P-18:1/20:4)	5.48	748.5	303.3
PE(P-18:1/22:6)	5.29	772.5	327.3
PEth(16:0_18:1)	5.4	772.5	255.1
PEth(18:1/18:1)	5.4	773.6	281.2
PG(15:0/18:1(d7))	4.87	740.6	241.3
PG(16:0_18:1)	5.13	747.5	255.3
PG(16:0_20:4)	4.61	769.5	255.3
PG(16:0_22:6)	4.28	795.5	255.3
PG(18:0_18:1)	5.6	775.5	281.3
PG(18:0_20:4)	5.14	797.5	283.3
PG(18:0_22:6)	4.79	823.5	283.3
PG(18:1/18:1)	5.14	773.4	281.4
PI(15:0/18:1(d7))	4.87	828.6	241.3
PI(16:0_18:1)	5.04	835.6	255.3
PI(16:0_20:4)	4.61	857.6	255.3
PI(16:0_22:6)	4.43	881.6	255.3
PI(18:0_18:1)	5.51	863.6	283.3
PI(18:0_20:4)	5.12	885.6	283.3
PI(18:0_22:6)	4.97	909.6	283.3
PI(18:1/18:1)	5.04	861.6	281.3
PI(20:4/20:4)	3.96	905.6	303.3
PS(15:0/18:1(d7))	4.94	753.6	241.3
PS(16:0_18:1)	5.19	760.6	255.3
PS(16:0_20:4)	4.68	782.6	255.3
PS(16:0_22:6)	4.5	806.6	255.3
PS(18:0_18:1)	5.66	788.6	283.3
PS(18:0_20:4)	5.25	810.6	283.3
PS(18:0_22:6)	5.02	834.6	283.3
PS(18:1/18:1)	5.19	786.6	281.3
PS(22:6/22:6)	3.66	878.5	327.3
Stearic acid	3.6	283.2	283.2
PE(P-18:0/18:1(d9))	6.4	737.6	290.2
METABOLITES_END
#END