#METABOLOMICS WORKBENCH Jung_DNLI_20250905_111043 DATATRACK_ID:6386 STUDY_ID:ST004205 ANALYSIS_ID:AN006994 PROJECT_ID:PR002651
VERSION             	1
CREATED_ON             	September 19, 2025, 6:13 pm
#PROJECT
PR:PROJECT_TITLE                 	GPR34 regulates microglia state and loss-of-function rescues TREM2 metabolic
PR:PROJECT_TITLE                 	dysfunction
PR:PROJECT_TYPE                  	cellular study
PR:PROJECT_SUMMARY               	Microglia are implicated in modifying neurodegenerative disease risk in the
PR:PROJECT_SUMMARY               	central nervous system (CNS). GPR34 is a microglia-enriched G-protein coupled
PR:PROJECT_SUMMARY               	receptor that detects cytotoxic lipids upregulated in Alzheimer’s Disease
PR:PROJECT_SUMMARY               	(AD). Since dysregulated lipid metabolism occurs in disease, we hypothesized
PR:PROJECT_SUMMARY               	GPR34 could act with other lipid sensors, such as TREM2, to regulate microglial
PR:PROJECT_SUMMARY               	function. Here, we report that GPR34 knockout (KO) rescues dysregulated
PR:PROJECT_SUMMARY               	cholesterol metabolism in TREM2 KO iPSC-derived microglia (iMG) and alone
PR:PROJECT_SUMMARY               	promotes fatty acid catabolism without the proton leak observed in TREM2 KO.
PR:PROJECT_SUMMARY               	Loss of GPR34 downregulated ERK signaling, while its agonism promoted
PR:PROJECT_SUMMARY               	interaction with and activation of ERK. In healthy and amyloid mouse models,
PR:PROJECT_SUMMARY               	Gpr34 KO accelerated the conversion of homeostatic microglia to
PR:PROJECT_SUMMARY               	disease-associated microglia (DAM) states. Additionally, in Gpr34 KO amyloid
PR:PROJECT_SUMMARY               	mouse brain, the frequency of large plaques was increased compared to WT,
PR:PROJECT_SUMMARY               	indicating that Gpr34 KO microglia may promote amyloid aggregation. Overall, our
PR:PROJECT_SUMMARY               	data suggest GPR34 as a therapeutic target for modulating microglial function to
PR:PROJECT_SUMMARY               	slow AD progression.
PR:INSTITUTE                     	Denali Therapeutics
PR:LAST_NAME                     	Suh
PR:FIRST_NAME                    	Jung
PR:ADDRESS                       	161 Oyster Point Blvd, South San Francisco, California, 94080, USA
PR:EMAIL                         	suh@dnli.com
PR:PHONE                         	+1 6507973837
#STUDY
ST:STUDY_TITLE                   	Lipidomic/Metabolomic Characterization of GPR34 KO, WT, TREM2 KO, and
ST:STUDY_TITLE                   	GPR34/TREM2 dKO iPSC1-derived microglia
ST:STUDY_SUMMARY                 	GPR34 is a microglia-enriched receptor that senses cytotoxic lipids in
ST:STUDY_SUMMARY                 	Alzheimer’s disease and may interact with other lipid sensors like TREM2 to
ST:STUDY_SUMMARY                 	regulate metabolism. Using targeted lipid and metabolite profiling of
ST:STUDY_SUMMARY                 	iPSC-derived microglia with GPR34 KO, TREM2 KO, or GPR34/TREM2 double KO, we
ST:STUDY_SUMMARY                 	found that GPR34 deletion restores cholesterol metabolism defects in TREM2 KO
ST:STUDY_SUMMARY                 	cells and independently enhances fatty acid catabolism.
ST:INSTITUTE                     	Denali Therapeutics
ST:LAST_NAME                     	Suh
ST:FIRST_NAME                    	Jung
ST:ADDRESS                       	161 Oyster Point Blvd
ST:EMAIL                         	suh@dnli.com
ST:PHONE                         	+1 6507973837
#SUBJECT
SU:SUBJECT_TYPE                  	Human
SU:SUBJECT_SPECIES               	Homo sapiens
SU:TAXONOMY_ID                   	9606
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data
SUBJECT_SAMPLE_FACTORS           	Pool_1	HSA-000028400	Genotype:NA | Treatment:NA | Sample source:iMicroglia	RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_001; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_001; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_001
SUBJECT_SAMPLE_FACTORS           	WT3 Myelin	HSA-000028164	Genotype:WT | Treatment:Myelin | Sample source:iMicroglia	RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_002; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_002; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_002
SUBJECT_SAMPLE_FACTORS           	GPR34KO4 Myelin	HSA-000028179	Genotype:GPR34-KO | Treatment:Myelin | Sample source:iMicroglia	RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_004; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_004; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_004
SUBJECT_SAMPLE_FACTORS           	dKO A4 4 Myelin	HSA-000028185	Genotype:GPR34/TREM2-KO | Treatment:Myelin | Sample source:iMicroglia	RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_005; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_005; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_005
SUBJECT_SAMPLE_FACTORS           	dKO A4 3 Myelin	HSA-000028173	Genotype:GPR34/TREM2-KO | Treatment:Myelin | Sample source:iMicroglia	RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_006; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_006; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_006
SUBJECT_SAMPLE_FACTORS           	TREM2KO1 Myelin	HSA-000028146	Genotype:TREM2-KO | Treatment:Myelin | Sample source:iMicroglia	RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_007; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_007; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_007
SUBJECT_SAMPLE_FACTORS           	GPR34KO1 Myelin	HSA-000028143	Genotype:GPR34-KO | Treatment:Myelin | Sample source:iMicroglia	RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_009; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_009; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_009
SUBJECT_SAMPLE_FACTORS           	dKO A4 4 PBS	HSA-000028184	Genotype:GPR34/TREM2-KO | Treatment:PBS | Sample source:iMicroglia	RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_010; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_010; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_010
SUBJECT_SAMPLE_FACTORS           	TREM2KO3 PBS	HSA-000028169	Genotype:TREM2-KO | Treatment:PBS | Sample source:iMicroglia	RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_011; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_011; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_011
SUBJECT_SAMPLE_FACTORS           	GPR34KO3 Myelin	HSA-000028167	Genotype:GPR34-KO | Treatment:Myelin | Sample source:iMicroglia	RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_012; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_012; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_012
SUBJECT_SAMPLE_FACTORS           	TREM2KO2 PBS	HSA-000028157	Genotype:TREM2-KO | Treatment:PBS | Sample source:iMicroglia	RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_013; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_013; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_013
SUBJECT_SAMPLE_FACTORS           	TREM2KO4 Myelin	HSA-000028182	Genotype:TREM2-KO | Treatment:Myelin | Sample source:iMicroglia	RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_016; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_016; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_016
SUBJECT_SAMPLE_FACTORS           	Pool_2	HSA-000028401	Genotype:NA | Treatment:NA | Sample source:iMicroglia	RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_017; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_017; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_017
SUBJECT_SAMPLE_FACTORS           	WT3 PBS	HSA-000028163	Genotype:WT | Treatment:PBS | Sample source:iMicroglia	RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_019; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_019; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_019
SUBJECT_SAMPLE_FACTORS           	TREM2KO2 Myelin	HSA-000028158	Genotype:TREM2-KO | Treatment:Myelin | Sample source:iMicroglia	RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_020; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_020; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_020
SUBJECT_SAMPLE_FACTORS           	dKO A4 2 Myelin	HSA-000028161	Genotype:GPR34/TREM2-KO | Treatment:Myelin | Sample source:iMicroglia	RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_021; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_021; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_021
SUBJECT_SAMPLE_FACTORS           	GPR34KO3 PBS	HSA-000028166	Genotype:GPR34 KO | Treatment:PBS | Sample source:iMicroglia	RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_022; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_022; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_022
SUBJECT_SAMPLE_FACTORS           	WT2 PBS	HSA-000028151	Genotype:WT | Treatment:PBS | Sample source:iMicroglia	RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_023; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_023; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_023
SUBJECT_SAMPLE_FACTORS           	GPR34KO4 PBS	HSA-000028178	Genotype:GPR34-KO | Treatment:PBS | Sample source:iMicroglia	RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_024; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_024; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_024
SUBJECT_SAMPLE_FACTORS           	TREM2KO3 Myelin	HSA-000028170	Genotype:TREM2-KO | Treatment:Myelin | Sample source:iMicroglia	RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_026; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_026; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_026
SUBJECT_SAMPLE_FACTORS           	WT2 Myelin	HSA-000028152	Genotype:WT | Treatment:Myelin | Sample source:iMicroglia	RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_027; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_027; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_027
SUBJECT_SAMPLE_FACTORS           	GPR34KO1 PBS	HSA-000028142	Genotype:GPR34-KO | Treatment:PBS | Sample source:iMicroglia	RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_028; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_028; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_028
SUBJECT_SAMPLE_FACTORS           	WT1 Myelin	HSA-000028140	Genotype:WT | Treatment:Myelin | Sample source:iMicroglia	RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_031; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_031; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_031
SUBJECT_SAMPLE_FACTORS           	dKO A4 1 Myelin	HSA-000028149	Genotype:GPR34/TREM2-KO | Treatment:Myelin | Sample source:iMicroglia	RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_032; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_032; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_032
SUBJECT_SAMPLE_FACTORS           	Pool_3	HSA-000028402	Genotype:NA | Treatment:NA | Sample source:iMicroglia	RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_035; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_035; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_035
SUBJECT_SAMPLE_FACTORS           	dKO A4 1 PBS	HSA-000028148	Genotype:GPR34/TREM2-KO | Treatment:PBS | Sample source:iMicroglia	RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_036; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_036; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_036
SUBJECT_SAMPLE_FACTORS           	dKO A4 3 PBS	HSA-000028172	Genotype:GPR34/TREM2-KO | Treatment:PBS | Sample source:iMicroglia	RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_038; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_038; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_038
SUBJECT_SAMPLE_FACTORS           	dKO A4 2 PBS	HSA-000028160	Genotype:GPR34/TREM2-KO | Treatment:PBS | Sample source:iMicroglia	RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_041; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_041; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_041
SUBJECT_SAMPLE_FACTORS           	TREM2KO1 PBS	HSA-000028145	Genotype:TREM2-KO | Treatment:PBS | Sample source:iMicroglia	RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_043; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_043; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_043
SUBJECT_SAMPLE_FACTORS           	WT4 Myelin	HSA-000028176	Genotype:WT | Treatment:Myelin | Sample source:iMicroglia	RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_045; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_045; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_045
SUBJECT_SAMPLE_FACTORS           	WT1 PBS	HSA-000028139	Genotype:WT | Treatment:PBS | Sample source:iMicroglia	RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_046; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_046; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_046
SUBJECT_SAMPLE_FACTORS           	WT4 PBS	HSA-000028175	Genotype:WT | Treatment:PBS | Sample source:iMicroglia	RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_047; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_047; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_047
SUBJECT_SAMPLE_FACTORS           	GPR34KO2 PBS	HSA-000028154	Genotype:GPR34-KO | Treatment:PBS | Sample source:iMicroglia	RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_049; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_049; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_049
SUBJECT_SAMPLE_FACTORS           	TREM2KO4 PBS	HSA-000028181	Genotype:TREM2-KO | Treatment:PBS | Sample source:iMicroglia	RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_050; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_050; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_050
SUBJECT_SAMPLE_FACTORS           	GPR34KO2 Myelin	HSA-000028155	Genotype:GPR34-KO | Treatment:Myelin | Sample source:iMicroglia	RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_051; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_051; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_051
SUBJECT_SAMPLE_FACTORS           	Pool_4	HSA-000028403	Genotype:NA | Treatment:NA | Sample source:iMicroglia	RAW_FILE_NAME(Raw Source Name.LN)=20230127_KR_HEX-000537_Lipid_Neg_052; RAW_FILE_NAME(Raw Source Name.LP)=20230127_KR_HEX-000537_Lipid_Pos_052; RAW_FILE_NAME(Raw Source Name.MP)=20230127_KR_HEX-000537_Met_Pos_052
#COLLECTION
CO:COLLECTION_SUMMARY            	iPSC1-derived microglia were harvested by aspirating medium then briefly washed
CO:COLLECTION_SUMMARY            	with 1mL of ice-cold PBS solution and directly processed for lipidomic and
CO:COLLECTION_SUMMARY            	metabolomic analysis.
CO:SAMPLE_TYPE                   	IPSC1-derived microglia
CO:STORAGE_CONDITIONS            	-80℃
CO:COLLECTION_VIALS              	Lobind 1.5 mL Eppendorf tubes
CO:STORAGE_VIALS                 	Lobind 1.5 mL Eppendorf tubes
#TREATMENT
TR:TREATMENT_SUMMARY             	Demyelination is a key feature of neuronal degeneration in vivo in AD and
TR:TREATMENT_SUMMARY             	amyloid mouse models (see Nugent et al. 2020,
TR:TREATMENT_SUMMARY             	https://doi.org/10.1016/j.neuron.2019.12.007). For this reason, we selected it
TR:TREATMENT_SUMMARY             	as a stimulus to evaluate microglial metabolism under a physiologically relevant
TR:TREATMENT_SUMMARY             	stressor in vitro. Cells were treated for 48 hours with PBS or Myelin, followed
TR:TREATMENT_SUMMARY             	by lipid extraction.
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	Cells were harvested by aspirating the medium, briefly washed with 1 mL of
SP:SAMPLEPREP_SUMMARY            	ice-cold PBS, and then lysed in 50 µL methanol containing stable-isotope
SP:SAMPLEPREP_SUMMARY            	internal standards. Samples were vortexed, adjusted to 100 µl with MS-grade
SP:SAMPLEPREP_SUMMARY            	H₂O, and transferred to 1.5 ml Protein LoBind tubes on ice. 100 µl of Methyl
SP:SAMPLEPREP_SUMMARY            	tert-butyl ether (MTBE)was added, vortexed and centrifuged at 21,000 g for 10
SP:SAMPLEPREP_SUMMARY            	min at 4 °C. The two phases were separated: the top (non-polar lipids) and
SP:SAMPLEPREP_SUMMARY            	bottom (polar metabolites) were collected into glass vials, dried overnight in a
SP:SAMPLEPREP_SUMMARY            	Genevac EZ3, and resuspended in 100 µl of methanol (lipids) or 9:1
SP:SAMPLEPREP_SUMMARY            	methanol:H₂O (metabolites) for LC-MS/MS analysis.
SP:PROCESSING_STORAGE_CONDITIONS 	On ice
SP:EXTRACT_STORAGE               	-20℃
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_TYPE           	HILIC
CH:INSTRUMENT_NAME               	Agilent 1290 Infinity II
CH:COLUMN_NAME                   	Waters ACQUITY UPLC BEH Amide (150 x 2.1mm,1.7um)
CH:SOLVENT_A                     	60% acetonitrile/40% water;10 mM ammonium formate; 0.1% formic acid
CH:SOLVENT_B                     	90% isopropyl alcohol/ 10% acetonitrile;10 mM ammonium formate; 0.1% formic acid
CH:FLOW_GRADIENT                 	0.0-8.0 min from 45% B to 99% B, 8.0-9.0 min at 99% B, 9.0-9.1 min to 45% B, and
CH:FLOW_GRADIENT                 	9.1-10.0 min at 45% B.
CH:FLOW_RATE                     	0.25 mL/min
CH:COLUMN_TEMPERATURE            	55
CH:SAMPLE_INJECTION              	8
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
#MS
MS:INSTRUMENT_NAME               	ABI Sciex 6500+ QTrap
MS:INSTRUMENT_TYPE               	Triple quadrupole
MS:MS_TYPE                       	ESI
MS:ION_MODE                      	POSITIVE
MS:MS_COMMENTS                   	curtain gas at 30 psi; collision gas was set at medium; ion spray voltage at
MS:MS_COMMENTS                   	5,500 V; temperature at 600°C; ion source Gas 1 at 50 psi; ion source Gas 2 at
MS:MS_COMMENTS                   	60 psi
#MS_METABOLITE_DATA
MS_METABOLITE_DATA:UNITS	normalized peak area
MS_METABOLITE_DATA_START
Samples	HSA-000028139	HSA-000028140	HSA-000028142	HSA-000028143	HSA-000028145	HSA-000028146	HSA-000028148	HSA-000028149	HSA-000028151	HSA-000028152	HSA-000028154	HSA-000028155	HSA-000028157	HSA-000028158	HSA-000028160	HSA-000028161	HSA-000028163	HSA-000028164	HSA-000028166	HSA-000028167	HSA-000028169	HSA-000028170	HSA-000028172	HSA-000028173	HSA-000028175	HSA-000028176	HSA-000028178	HSA-000028179	HSA-000028181	HSA-000028182	HSA-000028184	HSA-000028185	HSA-000028400	HSA-000028401	HSA-000028402	HSA-000028403
Factors	Genotype:WT | Treatment:PBS | Sample source:iMicroglia	Genotype:WT | Treatment:Myelin | Sample source:iMicroglia	Genotype:GPR34-KO | Treatment:PBS | Sample source:iMicroglia	Genotype:GPR34-KO | Treatment:Myelin | Sample source:iMicroglia	Genotype:TREM2-KO | Treatment:PBS | Sample source:iMicroglia	Genotype:TREM2-KO | Treatment:Myelin | Sample source:iMicroglia	Genotype:GPR34/TREM2-KO | Treatment:PBS | Sample source:iMicroglia	Genotype:GPR34/TREM2-KO | Treatment:Myelin | Sample source:iMicroglia	Genotype:WT | Treatment:PBS | Sample source:iMicroglia	Genotype:WT | Treatment:Myelin | Sample source:iMicroglia	Genotype:GPR34-KO | Treatment:PBS | Sample source:iMicroglia	Genotype:GPR34-KO | Treatment:Myelin | Sample source:iMicroglia	Genotype:TREM2-KO | Treatment:PBS | Sample source:iMicroglia	Genotype:TREM2-KO | Treatment:Myelin | Sample source:iMicroglia	Genotype:GPR34/TREM2-KO | Treatment:PBS | Sample source:iMicroglia	Genotype:GPR34/TREM2-KO | Treatment:Myelin | Sample source:iMicroglia	Genotype:WT | Treatment:PBS | Sample source:iMicroglia	Genotype:WT | Treatment:Myelin | Sample source:iMicroglia	Genotype:GPR34 KO | Treatment:PBS | Sample source:iMicroglia	Genotype:GPR34-KO | Treatment:Myelin | Sample source:iMicroglia	Genotype:TREM2-KO | Treatment:PBS | Sample source:iMicroglia	Genotype:TREM2-KO | Treatment:Myelin | Sample source:iMicroglia	Genotype:GPR34/TREM2-KO | Treatment:PBS | Sample source:iMicroglia	Genotype:GPR34/TREM2-KO | Treatment:Myelin | Sample source:iMicroglia	Genotype:WT | Treatment:PBS | Sample source:iMicroglia	Genotype:WT | Treatment:Myelin | Sample source:iMicroglia	Genotype:GPR34-KO | Treatment:PBS | Sample source:iMicroglia	Genotype:GPR34-KO | Treatment:Myelin | Sample source:iMicroglia	Genotype:TREM2-KO | Treatment:PBS | Sample source:iMicroglia	Genotype:TREM2-KO | Treatment:Myelin | Sample source:iMicroglia	Genotype:GPR34/TREM2-KO | Treatment:PBS | Sample source:iMicroglia	Genotype:GPR34/TREM2-KO | Treatment:Myelin | Sample source:iMicroglia	Genotype:NA | Treatment:NA | Sample source:iMicroglia	Genotype:NA | Treatment:NA | Sample source:iMicroglia	Genotype:NA | Treatment:NA | Sample source:iMicroglia	Genotype:NA | Treatment:NA | Sample source:iMicroglia
1,3-15N2-Uric acid	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
1-Methylhistidine	0.003	0.005	0.002	0.005	0.001	0.003	0.002	0.004	0.005	0.006	0.008	0.005	0.003	0.004	0.002	0.002	0.004	0.007	0.006	0.006	0.003	0.004	0.003	0.004	0.004	0.004	0.004	0.006	0.003	0.003	0.004	0.004	0.005	0.004	0.004	0.004
1-Methylnicotinamide	0.073	0.066	0.108	0.165	0.054	0.166	0.216	0.234	0.072	0.071	0.279	0.29	0.218	0.267	0.069	0.14	0.074	0.084	0.4	0.421	0.282	0.31	0.211	0.265	0.159	0.1	0.377	0.624	0.547	0.408	0.436	0.5	0.364	0.369	0.398	0.406
1-Methylxanthine	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
1-Methylxanthosine	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
13C10,15N5-Adenosine	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
13C3-Thiamine	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
13C5-Hypoxanthine	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
13C5-Xanthosine	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
15N4-Inosine	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
2-Aminobenzoic acid	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
3-Hydroxy-N6,N6,N6-Trimethyllysine	0.002	0.002	0.001	0.002	0.001	0.003	0.003	0.003	0.002	0.003	0.004	0.004	0.005	0.005	0.001	0.002	0.002	0.003	0.005	0.006	0.005	0.005	0.004	0.004	0.002	0.002	0.004	0.007	0.007	0.008	0.006	0.007	0.004	0.004	0.004	0.004
3-Hydroxybutyric acid	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
3-Hydroxykynurenine	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
3-Hydroxytyrosol	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
3-Methoxytyrosine	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
3-Methylxanthine	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
3-Methylxanthosine	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
4-Hydroxyproline	0.011	0.017	0.008	0.015	0.004	0.013	0.013	0.022	0.02	0.03	0.031	0.028	0.012	0.012	0.011	0.011	0.016	0.012	0.026	0.029	0.015	0.015	0.014	0.014	0.016	0.013	0.015	0.023	0.012	0.013	0.014	0.017	0.018	0.018	0.014	0.016
4-Trimethylammoniobutanal	0.001	0.001	0.001	0.001	0	0.001	0.001	0.002	0.001	0.002	0.002	0.002	0.001	0.001	0.001	0.001	0.001	0.001	0.002	0.002	0.001	0.001	0.001	6.94E-05	0.004	0.003	0.004	0.004	0.004	0.004	0.003	0.004	0.002	0.002	0.002	0.002
5'-Methylthioadenosine	0.239	0.311	0.212	0.282	0.102	0.181	0.3	0.347	0.324	0.43	0.484	0.395	0.188	0.279	0.14	0.208	0.303	0.367	0.531	0.54	0.287	0.304	0.295	0.423	0.362	0.757	0.311	1.361	0.323	0.305	0.455	0.485	0.412	0.568	0.535	0.649
5-Glutamylalanine	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
5-Hydroxyindoleacetic acid	0.001	0.001	0	0.001	0	0.001	0.001	0.001	0.001	0.002	0.001	0.002	0.001	0.001	0.001	0	0.001	0.001	0.001	0.001	0	0.001	0.001	0	0.001	0.001	0.001	0.001	0.001	0.001	0.001	0.001	0.001	0.001	0.001	0.001
5-Hydroxytryptophan	0.002	0.004	0.002	0.004	0.002	0.003	0.002	0.004	0.003	0.005	0.004	0.004	0.001	0.002	0.002	0.002	0.002	0.003	0.005	0.004	N.D.	0.003	0.002	0.002	0.003	0.004	0.003	0.003	0.002	0.002	0.002	0.003	0.002	0.003	0.003	0.003
6-Aminouracil	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
6C13-Phenylalanine	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
6C13-Tyrosine	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
7-Methylguanine	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
7-Methylguanosine	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
7-Methylxanthine	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
7-Methylxanthosine	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
7-alpha-Hydroxy-3-oxo-4-cholestenoate	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
8-Hydroxy-deoxyguanosine	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
8-Hydroxyguanosine	0.01	0.01	0.009	0.011	0.01	0.01	0.01	0.008	0.008	0.004	0.008	0.007	0.007	0.008	0.009	0.011	0.012	0.015	0.008	0.008	0.006	0.008	0.007	0.009	0.007	0.012	0.009	0.011	0.007	0.008	0.01	0.012	0.017	0.017	0.018	0.016
8-Oxo-deoxyguanosine-13C,15N2	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
9-Hexadecenoylcarnitine	0.049	0.051	0.031	0.034	0.01	0.02	0.022	0.037	0.054	0.053	0.044	0.047	0.017	0.023	0.011	0.017	0.022	0.028	0.033	0.033	0.017	0.026	0.02	0.025	0.038	0.053	0.02	0.026	0.024	0.024	0.019	0.025	0.021	0.024	0.026	0.027
AICA-riboside	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Acetylcarnitine	0.047	0.053	0.041	0.051	0.014	0.036	0.048	0.058	0.053	0.066	0.103	0.115	0.045	0.06	0.02	0.027	0.038	0.048	0.081	0.093	0.041	0.05	0.04	0.05	0.054	0.046	0.065	0.081	0.069	0.057	0.058	0.066	0.051	0.053	0.055	0.053
Acetylcarnitine-d3	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Adenine	0.487	0.331	0.324	0.436	0.464	0.494	0.51	0.453	0.344	0.368	0.685	0.733	0.435	0.54	0.511	0.203	0.36	0.343	0.692	0.754	0.547	0.702	0.444	0.418	0.914	0.546	1.145	0.746	1.908	1.066	1.398	1.324	0.449	0.417	0.404	0.484
Adenosine	0.065	0.087	0.048	0.066	0.018	0.068	0.067	0.082	0.072	0.07	0.094	0.101	0.04	0.082	0.016	0.021	0.086	0.07	0.11	0.137	0.052	0.092	0.044	0.069	0.176	0.091	0.209	0.078	0.231	0.159	0.185	0.232	0.073	0.069	0.07	0.073
Adenosine diphosphate ribose	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Adenosylcobalamin	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Alanine	0.075	0.089	0.047	0.079	0.022	0.055	0.053	0.084	0.107	0.137	0.15	0.135	0.053	0.066	0.041	0.043	0.1	0.097	0.137	0.139	0.061	0.061	0.06	0.066	0.111	0.081	0.101	0.128	0.1	0.067	0.082	0.094	0.077	0.083	0.083	0.091
Alanine-d4	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Arabitol	0.536	0.582	0.53	0.55	0.508	0.492	0.514	0.18	0.757	0.89	0.665	0.644	0.6	0.626	0.572	0.583	0.597	0.377	0.556	0.508	0.532	0.512	0.485	0.418	0.812	0.681	0.641	0.644	0.615	0.599	0.571	0.475	0.519	0.533	0.548	0.583
Arginine	0.086	0.143	0.073	0.13	0.046	0.105	0.083	0.151	0.118	0.219	0.207	0.193	0.088	0.142	0.086	0.108	0.115	0.134	0.166	0.165	0.099	0.119	0.094	0.109	0.359	0.358	0.386	0.357	0.344	0.318	0.338	0.356	0.17	0.176	0.184	0.184
Arginine-d4C13	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Ascorbate	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Asparagine	1.088	1.151	0.807	0.896	0.359	0.819	0.728	1.086	1.419	1.609	1.48	1.256	0.624	0.778	0.514	0.545	1.374	1.378	1.383	1.495	0.64	0.68	0.723	0.784	1.259	0.876	0.952	1.026	0.97	0.68	0.955	0.889	0.957	0.914	0.916	0.973
Aspartic acid	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Aspartic acid-d3	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Asymmetric dimethylarginine	4.6	6.756	3.914	5.524	1.835	5.029	4.364	6.129	6.449	9.447	8.117	6.32	4.497	5.704	3.588	3.521	6.88	7.789	7.02	7.14	5.111	4.653	4.218	4.846	7.318	5.329	6.609	6.509	5.546	5.183	5.298	5.707	5.991	5.726	5.141	5.43
Betaine	0.924	0.941	0.598	0.811	0.363	0.917	1.158	1.252	1.373	1.378	1.404	1.153	1.167	1.259	0.633	0.611	1.036	1.071	1.33	1.265	0.942	0.976	1.062	1.07	1.184	0.859	0.991	0.99	1.508	1.315	1.391	1.405	1.07	1.113	0.989	1.025
Betaine-d11	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Bilirubin	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Butyrobetaine	0.06	0.053	0.031	0.032	0.016	0.031	0.064	0.073	0.094	0.084	0.131	0.115	0.049	0.054	0.021	0.02	0.057	0.05	0.135	0.112	0.05	0.052	0.065	0.054	0.078	0.054	0.077	0.08	0.121	0.062	0.083	0.084	0.052	0.064	0.076	0.079
Butyrylcarnitine	0.02	0.023	0.018	0.024	0.007	0.017	0.027	0.032	0.023	0.032	0.051	0.053	0.022	0.024	0.01	0.012	0.018	0.022	0.054	0.059	0.024	0.025	0.027	0.03	0.021	0.023	0.025	0.032	0.031	0.027	0.032	0.037	0.027	0.027	0.027	0.027
Butyrylcarnitine-d3	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Cadaverine	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Caffeine	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Capsaicin	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Carnitine	0.07	0.078	0.038	0.052	0.018	0.041	0.051	0.056	0.117	0.103	0.102	0.087	0.044	0.044	0.018	0.018	0.085	0.088	0.171	0.163	0.049	0.048	0.054	0.056	0.057	0.046	0.059	0.064	0.053	0.041	0.063	0.056	0.073	0.071	0.072	0.069
Carnitine-d9	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Choline	0.158	0.154	0.128	0.149	0.043	0.087	0.133	0.136	0.202	0.189	0.242	0.235	0.125	0.131	0.069	0.081	0.168	0.161	0.27	0.254	0.135	0.135	0.142	0.122	0.171	0.138	0.195	0.178	0.164	0.13	0.171	0.141	0.161	0.161	0.158	0.16
Citrulline	0.011	0.014	0.008	0.018	0.005	0.008	0.009	0.014	0.015	0.018	0.018	0.017	0.007	0.009	0.006	0.005	0.009	0.028	0.017	0.02	0.007	0.009	0.013	0.012	0.01	0.01	0.009	0.123	0.012	0.007	0.007	0.01	0.013	0.015	0.015	0.011
Citrulline-d2	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Cotinine	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Creatine	0.864	0.977	0.483	0.639	0.204	0.449	0.663	0.8	0.986	1.109	1.179	1.089	0.432	0.562	0.314	0.343	0.925	1.189	1.002	1.075	0.481	0.552	0.594	0.544	0.912	0.741	0.588	0.463	0.704	0.534	0.613	0.615	0.607	0.582	0.634	0.672
Creatinine	0.049	0.065	0.034	0.052	0.02	0.038	0.038	0.054	0.062	0.081	0.06	0.056	0.031	0.039	0.029	0.041	0.056	0.071	0.054	0.052	0.03	0.03	0.031	0.035	0.063	0.082	0.05	0.086	0.031	0.03	0.039	0.044	0.074	0.076	0.077	0.08
Creatinine-d3	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Curcumin	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Cyanocobalamin	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Cystathionine	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Cysteine	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Cysteinylglycine	0.021	0.023	0.022	0.024	0.019	0.023	0.022	0.022	0.025	0.03	0.023	0.024	0.024	0.024	0.021	0.023	0.023	0.019	0.022	0.023	0.024	0.023	0.02	0.024	0.022	0.021	0.02	0.024	0.019	0.021	0.021	0.021	0.023	0.02	0.022	0.021
Cystine	0	0	0	0	0.001	0.001	0	0.001	0	0.001	0	0	0	0.001	0.001	0.001	0.001	N.D.	0	0	0	0.001	0	0	0.001	0.002	0.001	0.001	0	0.001	0	0.001	0.001	0.001	0.001	0.001
Cytosine	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
DNL201	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Decanoylcarnitine	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Demethoxycurcumin	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Deoxyadenosine	0.036	0.032	0.031	0.027	0.045	0.031	0.028	0.04	0.039	0.036	0.024	0.012	0.03	0.025	0.034	0.033	0.034	0.024	0.025	0.016	0.031	0.032	0.031	0.026	0.082	0.008	0.054	N.D.	0.164	0.004	0.04	0.02	0.028	0.028	0.025	0.022
Deoxyguanosine	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Deoxyinosine	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Dimethylethanolamine	0.012	0.015	0.01	0.012	0.006	0.008	0.009	0.011	0.012	0.016	0.01	0.014	0.009	0.014	0.009	0.013	0.019	0.02	0.01	0.011	0.008	0.009	0.01	0.01	0.031	0.035	0.02	0.033	0.014	0.017	0.016	0.02	0.029	0.032	0.033	0.037
Dimethylglycine	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Dodecanoylcarnitine	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Dopa	0.003	N.D.	0.001	0.001	0.002	0.002	0.003	0.004	N.D.	0.002	0.004	0.005	0.004	0.005	0.002	0.002	N.D.	N.D.	0.004	0.005	0.005	0.003	0.006	0.006	0.001	0.001	0.003	0.003	0.01	0.008	0.008	0.006	0.005	0.003	0.003	0.004
Dopamine	0.017	0.018	0.018	0.018	0.02	0.015	0.013	0.004	0.022	0.02	0.012	0.011	0.015	0.016	0.02	0.026	0.02	0.012	0.009	0.008	0.012	0.01	0.009	0.009	0.031	0.034	0.02	0.028	0.013	0.014	0.015	0.013	0.024	0.024	0.024	0.025
Dopamine 3-o-sulfate	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Dopamine 4-o-sulfate	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Epinephrine	0.012	0.016	0.009	0.013	0.004	0.01	0.011	0.015	0.019	0.023	0.024	0.024	0.01	0.013	0.007	0.008	0.015	0.018	0.024	0.024	0.01	0.011	0.01	0.011	0.015	0.014	0.014	0.017	0.012	0.011	0.013	0.014	0.014	0.014	0.014	0.014
Ergothioneine	0.041	0.046	0.029	0.043	0.006	0.018	0.031	0.034	0.049	0.054	0.06	0.066	0.017	0.02	0.008	0.01	0.041	0.046	0.089	0.093	0.019	0.02	0.037	0.027	0.032	0.028	0.034	0.036	0.02	0.018	0.026	0.027	0.035	0.033	0.031	0.03
Ethanolamine	0.003	0.012	0.004	0.014	0.002	0.02	0.009	0.019	0.004	0.013	0.014	0.033	0.008	0.031	0.004	0.007	0.004	0.013	0.019	0.034	0.01	0.031	0.011	0.02	0.007	0.009	0.011	0.018	0.017	0.039	0.015	0.03	0.007	0.007	0.007	0.007
Formylanthranilic acid	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Gamma-Glutamylcysteine	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Glucosamine	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Glucose	0.015	0.031	0.03	0.041	0.027	0.051	0.02	0.046	0.037	0.093	0.057	0.045	0.042	0.052	0.039	0.044	0.046	0.042	0.032	0.025	0.035	0.033	0.025	0.023	0.033	0.04	0.031	0.048	0.019	0.029	0.026	0.022	0.04	0.032	0.032	0.035
Glutamic acid	17.605	14.522	8.063	9.511	2.273	4.186	9.127	8.707	24.683	18.684	21.506	17.613	6.077	5.147	3.047	2.882	19.897	16.27	25.488	21.241	6.446	5.688	7.344	6.483	16.337	11.245	13.542	13.339	7.954	5.885	8.739	7.922	12.52	12.238	12.673	11.868
Glutamic acid-d3	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Glutamine	11.392	13.963	7.331	10.571	3.75	8.28	8.163	11.357	15.736	19.627	16.654	15.458	7.746	9.17	6.028	6.298	13.466	15.554	15.708	15.058	7.603	8.071	7.939	9.401	11.258	10.139	9.254	11.265	7.673	7.684	9.124	9.157	11.168	10.279	10.689	10.499
Glutathione	11.995	12.697	7.718	9.891	1.547	5.067	8.86	7.884	14.535	14.68	12.702	13.661	4.393	5.782	1.894	2.454	9.882	15.247	19.291	19.509	4.812	5.836	6.141	9.401	3.893	0.492	4.142	0.408	3.398	3.062	5.511	4.465	7.685	7.296	7.257	6.959
Glutathione disulfide	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Glutathione-(glycine-13C2,15N)	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Glycerophosphocholine	95.837	125.801	154.445	184.217	30.857	114.608	118.571	120.854	105.593	112.455	188.483	148.98	94.489	98.421	32.139	41.743	147.062	141.874	299.602	330.387	138.055	129.308	118.981	133.547	64.945	63.6	131.91	149.681	80.007	100.783	94.016	95.848	148.715	142.436	125.922	121.987
Glycine	0.162	0.194	0.099	0.127	0.056	0.109	0.136	0.137	0.206	0.245	0.218	0.226	0.094	0.12	0.072	0.065	0.187	0.209	0.25	0.242	0.103	0.119	0.117	0.128	0.176	0.156	0.176	0.204	0.143	0.145	0.128	0.122	0.146	0.14	0.131	0.148
Guanine	0.006	0.007	0.006	0.006	0.004	0.004	0.006	0.002	0.004	0.005	0.008	0.004	0.004	0.004	0.004	0.005	0.011	0.004	0.005	0.004	0.005	0.005	0.005	0.004	0.013	0.011	0.009	0	0.01	0.004	0.006	0.005	0.008	0.009	0.01	0.01
Guanosine	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Hexanoylcarnitine	0.002	0.002	0.002	0.002	0.001	0.001	0.002	0.003	0.002	0.003	0.004	0.005	0.001	0.002	0.001	0.001	0.002	0.002	0.004	0.004	0.002	0.002	0.002	0.002	0.002	0.002	0.002	0.004	0.002	0.002	0.002	0.003	0.002	0.002	0.002	0.002
Histamine	N.D.	N.D.	1.676	3.451	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	4.604	4.835	0.224	0.396	N.D.	N.D.	N.D.	N.D.	4.939	5.087	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	1.808	3.553	N.D.	N.D.	N.D.	N.D.	1.089	0.998	1.05	0.918
Histidine	0.105	0.123	0.069	0.136	0.033	0.092	0.086	0.149	0.166	0.21	0.203	0.156	0.088	0.118	0.075	0.073	0.136	N.D.	0.177	0.184	0.089	0.099	0.099	0.102	0.121	0.119	0.136	0.161	0.102	0.106	0.114	0.13	0.123	0.115	0.117	0.112
Homocysteine	0.337	0.632	0.428	0.551	0.285	0.465	0.301	0.393	1.729	2.376	1.145	1.193	1.476	1.521	0.991	1.628	0.781	0.522	0.728	0.513	0.512	0.469	0.358	0.675	1.917	1.675	1.815	1.725	0.973	1.223	0.983	0.64	1.058	1.061	0.995	0.916
Homoserine	8.686	10.396	6.291	9.044	2.808	6.584	6.677	8.445	12.456	15.703	13.839	12.809	6.221	7.876	4.442	4.961	10.754	13.283	13.766	14.887	6.309	6.325	6.297	7.121	10.032	9.164	8.245	10.186	6.732	7.147	7.216	7.686	8.827	8.989	8.52	8.074
Hydroxybutyrylcarnitine	0.021	0.021	0.014	0.017	0.007	0.017	0.021	0.026	0.022	0.027	0.046	0.054	0.012	0.026	0.008	0.01	0.016	0.02	0.041	0.044	0.02	0.024	0.019	0.023	0.023	0.021	0.028	0.031	0.037	0.029	0.026	0.034	0.022	0.023	0.027	0.025
Hydroxyhexanoylcarnitine	0.011	0.009	0.008	0.009	0.003	0.006	0.012	0.012	0.013	0.012	0.023	0.02	0.007	0.007	0.004	0.004	0.009	0.01	0.027	0.024	0.009	0.008	0.011	0.011	0.008	0.008	0.013	0.013	0.012	0.008	0.014	0.015	0.011	0.011	0.012	0.012
Hydroxyisovaleroyl carnitine	0.037	0.032	0.036	0.042	0.014	0.029	0.057	0.068	0.042	0.04	0.132	0.128	0.039	0.045	0.016	0.018	0.031	0.031	0.16	0.153	0.046	0.049	0.06	0.062	0.037	0.029	0.08	0.096	0.086	0.056	0.082	0.092	0.055	0.057	0.068	0.064
Hydroxypropionylcarnitine	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Hypoxanthine	0.037	0.052	0.094	0.077	0.024	0.059	0.112	0.124	0.014	0.008	0.13	0.082	0.065	0.055	0.029	0.033	0.065	0.036	0.148	0.113	0.08	0.097	0.101	0.073	0.014	0.019	0.048	0.041	0.061	0.045	0.059	0.047	0.067	0.058	0.054	0.061
Imidazoleacetic acid	0.152	0.076	0.115	0.085	0.184	0.101	0.169	0.12	0.03	0.026	0.055	0.049	0.031	0.032	0.06	0.033	0.066	0.095	0.074	0.1	0.097	0.094	0.146	0.073	0.023	0.033	0.028	0.028	0.051	0.038	0.054	0.087	0.052	0.055	0.057	0.062
Indole	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Inosine	0.001	0.002	0.002	0.001	0	0.001	0.002	0.003	0.001	0.001	0.002	0.002	0	0.001	0	0.001	0.002	0.001	0.002	0.002	0.001	0.001	0.001	0.001	0.005	0.003	0.005	0.002	0.003	0.001	0.001	0.002	0.002	0.003	0.003	0.003
Isovalerylcarnitine	0.075	0.07	0.122	0.15	0.042	0.093	0.217	0.241	0.089	0.091	0.461	0.394	0.163	0.155	0.06	0.073	0.058	0.061	0.492	0.469	0.169	0.158	0.228	0.24	0.066	0.05	0.221	0.271	0.249	0.177	0.273	0.302	0.186	0.189	0.195	0.183
Isovalerylcarnitine-d9	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Kynurenic acid	0	0.001	0	0	0	0	0	0	0.003	0.004	0.003	0.002	0.003	0.006	0.004	0.013	0.001	0	0	0	0	0	0	0	0.001	0.001	0.001	0.001	0	0	0	0	0.002	0.002	0.002	0.002
Kynurenine	0.008	0.006	0.002	0.003	0.001	0.002	0.003	0.004	0.004	0.003	0.002	0.002	0.001	0.001	0.001	0.001	0.007	0.006	0.002	0.003	0.002	0.002	0.003	0.003	0.003	0.003	0.001	0.001	0.001	0.001	0.001	0.002	0.002	0.003	0.003	0.003
LPC(18:1(d7))	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Leucine	0.217	0.271	0.152	0.224	0.067	0.154	0.165	0.223	0.323	0.394	0.396	0.382	0.154	0.198	0.117	0.126	0.271	0.301	0.387	0.404	0.16	0.176	0.165	0.185	0.293	0.239	0.267	0.26	0.245	0.19	0.22	0.245	0.24	0.239	0.234	0.228
Leucine-d3	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Linoleyl carnitine	0.004	0.003	0.004	0.003	0.002	0.003	0.003	0.003	0.005	0.004	0.004	0.004	0.003	0.004	0.003	0.003	0.002	0.002	0.003	0.003	0.004	0.005	0.004	0.004	0.003	0.014	0.004	0.007	0.003	0.005	0.002	0.003	0.004	0.004	0.005	0.005
Lysine	0.009	0.013	0.007	0.013	0.004	0.01	0.009	0.017	0.015	0.028	0.026	0.023	0.009	0.015	0.008	0.01	0.014	N.D.	0.024	0.023	0.01	0.015	0.011	0.011	0.017	0.015	0.02	0.017	0.016	0.013	0.014	0.016	0.012	0.014	0.014	0.014
Mannitol	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Mannose	0.007	0.015	0.011	0.018	0.012	0.024	0.01	0.021	0.017	0.037	0.029	0.025	0.019	0.026	0.019	0.02	0.019	0.02	0.019	0.014	0.017	0.014	0.012	0.012	0.016	0.015	0.017	0.022	0.009	0.012	0.012	0.01	0.017	0.016	0.016	0.015
Melatonin	0.001	0.002	0.001	0.002	0	0.001	0.002	0.002	0.002	0.002	0.002	0.002	0.001	0.001	0.001	0.001	0.001	0.002	0.002	0.003	0.001	0.001	0.001	0.002	0.002	0.003	0.002	0.004	0.002	0.002	0.002	0.003	0.003	0.003	0.003	0.003
Methionine	0.055	0.068	0.038	0.056	0.015	0.041	0.046	0.067	0.082	0.103	0.114	0.105	0.041	0.053	0.031	0.032	0.07	0.075	0.113	0.111	0.044	0.047	0.048	0.047	0.081	0.064	0.08	0.086	0.07	0.053	0.066	0.072	0.063	0.062	0.062	0.062
Methionine S-oxide	1.389	2.033	1.551	2.23	1.015	2.007	1.51	1.932	1.52	2.15	1.834	2.008	1.483	1.731	1.244	1.326	1.401	2.074	2.179	2.458	1.433	1.373	1.31	1.2	1.699	2.186	1.666	2.429	1.344	1.612	1.592	1.444	1.69	1.752	1.59	1.546
Methionine-d3	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Methylcobalamin	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Myristoylcarnitine	0.001	0.001	0	0.001	0	0.001	0	0.001	0.001	0.001	0.001	0.001	0	0.001	0	0.001	0	0.001	0.001	0.001	0	0.001	0	0.001	0	0.001	0	0.001	0.001	0.001	0.001	0.001	0.001	0.001	0.001	0.001
Myristoylcarnitine-d9	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
N,N'-Bis(gamma-glutamyl)cystine	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
N-Acetylalanine	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
N-Acetylaspartic acid	5.238	5.208	4.631	6.454	2.297	4.77	6.314	7.536	8.52	8.652	14.505	14.47	6.031	7.518	2.461	3.64	6.467	7.31	16.016	17.537	6.423	8.079	7.052	8.247	8.238	5.157	9.804	10.047	11.738	6.71	10.248	9.018	7.928	7.725	6.863	7.284
N-Acetylcysteine	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
N-Acetylglutamic acid	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
N-Acetylneuraminic acid	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
N-Acetylputrescine	0	0.001	0	0	0	0	0	0	0	0	0	0	0	N.D.	0	N.D.	0	0	0	0	0	0	0	0	0	0.001	0	N.D.	0	0	0	0	0	0	0	0
N-Acetylserine	0.002	0.003	0.001	0.001	0	0.001	0.002	0.002	0.003	0.005	0.003	0.003	0.001	0.002	0	0.001	0.003	0.003	0.003	0.003	0.001	0.001	0.001	0.001	0.003	0.002	0.002	0.002	0.002	0.001	0.001	0.002	0.002	0.002	0.002	0.002
N-Alpha-acetyllysine	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
N1-Acetylspermidine	0.732	0.802	0.561	1.508	0.166	1.114	2.126	3.487	1.497	2.733	4.672	5.785	2.06	4.623	0.377	1.357	0.99	1.132	5.644	7.645	2.689	4.749	2.75	3.442	2.776	3.192	1.939	2.343	9.739	5.405	8.216	8.053	2.402	2.547	2.429	2.481
N1-Acetylspermidine-d6	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
N1-Acetylspermine	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
N1-N12-Diacetylspermine	0.269	0.481	0.129	0.274	0.062	0.238	0.443	0.604	0.407	0.561	0.663	0.67	0.529	1.152	0.065	0.209	0.361	0.377	0.492	1.435	0.403	0.784	0.391	0.544	0.785	0.946	0.245	0.246	1.367	0.69	1.001	0.883	0.392	0.416	0.393	0.393
N1-N8-Diacetylspermidine	0.057	0.103	0.083	0.095	0.061	0.166	0.233	0.314	0.071	0.109	0.189	0.198	0.177	0.244	0.059	0.112	0.067	0.094	0.204	0.241	0.228	0.231	0.218	0.216	0.16	0.169	0.101	0.141	0.311	0.254	0.251	0.272	0.159	0.181	0.165	0.175
N1-N8-Diacetylspermidine-d6	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
N15C13-Glycine	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
N6,N6,N6-Trimethyllysine	0.008	0.009	0.005	0.008	0.001	0.004	0.005	0.009	0.011	0.016	0.015	0.013	0.005	0.008	0.004	0.005	0.01	0.014	0.013	0.013	0.005	0.007	0.005	0.005	0.009	0.01	0.009	0.009	0.008	0.006	0.007	0.007	0.007	0.008	0.008	0.008
NAD	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Niacinamide	0.019	0.025	0.017	0.023	0.01	0.02	0.022	0.03	0.025	0.043	0.048	0.05	0.019	0.027	0.018	0.018	0.027	0.026	0.046	0.046	0.022	0.024	0.022	0.028	0.033	0.035	0.029	0.082	0.025	0.019	0.023	0.021	0.027	0.028	0.028	0.027
Niacinamide-d4	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Nicotinamide mononucleotide	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Nicotinamide riboside	0.008	0.009	0.011	0.01	0.003	0.005	0.011	0.012	0.007	0.005	0.012	0.013	0.005	0.011	0.005	0.001	0.008	0.009	0.009	0.011	0.005	0.008	0.008	0.011	0.005	0.006	0.005	N.D.	0.011	0.007	0.007	0.006	0.007	0.008	0.01	0.009
Nicotinic acid	0	0	0	0	0	0	0	0	0	0.001	0.001	0.001	0	0	0	0	0	0	0	0.001	0	0	0	0	0	0	0	0.001	0.001	0	0	0	0	0	0	0
Nitrotyrosine	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Nonanoylcarnitine	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Norepinephrine	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Octanoylcarnitine	0	0.001	0	0.001	0	0	0.001	0.001	0.001	0.001	0.001	0.001	0	0	0	0	0	0.001	0.001	0	0.001	0.001	0	0.001	0.001	0.001	0.001	0.001	0	0	0.001	0.001	0.001	0.001	0	0
Octanoylcarnitine-d3	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Ornithine	0.015	0.022	0.02	0.027	0.008	0.029	0.013	0.022	0.018	0.036	0.035	0.022	0.016	0.019	0.012	0.014	0.018	N.D.	0.032	0.026	0.013	0.018	0.021	0.023	0.023	0.02	0.02	0.028	0.015	0.017	0.015	0.02	0.021	0.021	0.022	0.027
Ornithine-d2	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Palmitoylcarnitine	0.046	0.088	0.057	0.111	0.026	0.098	0.066	0.129	0.031	0.094	0.099	0.181	0.056	0.134	0.026	0.104	0.016	0.056	0.069	0.105	0.06	0.127	0.051	0.111	0.043	0.119	0.066	0.131	0.076	0.137	0.063	0.147	0.066	0.068	0.064	0.072
Palmitoylcarnitine-d3	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Paraxanthine	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Phenylalanine	0.649	0.841	0.486	0.715	0.224	0.538	0.574	0.801	0.993	1.211	1.255	1.257	0.519	0.678	0.397	0.471	0.806	0.906	1.255	1.28	0.566	0.593	0.571	0.621	0.826	0.755	0.757	0.86	0.66	0.615	0.709	0.768	0.741	0.754	0.756	0.745
Piperanine	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Piperine	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Pipernonaline	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Piperyline	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Proline	0.379	0.492	0.271	0.358	0.127	0.292	0.282	0.387	0.517	0.754	0.679	0.606	0.244	0.33	0.225	0.239	0.425	0.465	0.664	0.594	0.283	0.31	0.278	0.297	0.368	0.377	0.389	0.475	0.251	0.266	0.279	0.294	0.35	0.381	0.344	0.353
Propionylcarnitine	0.252	0.247	0.256	0.305	0.111	0.232	0.398	0.395	0.279	0.29	0.575	0.562	0.25	0.261	0.102	0.122	0.224	0.259	0.666	0.679	0.323	0.334	0.402	0.407	0.22	0.182	0.314	0.346	0.412	0.345	0.4	0.429	0.321	0.327	0.326	0.329
Propionylcartinine-d3	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Putrescine	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Pyridoxamine	1.23	1.262	1.021	1.311	0.715	1.018	1.088	1.274	1.427	1.45	1.496	1.494	1.049	1.232	0.801	0.441	1.186	1.48	1.362	1.482	1.024	1.102	0.927	1.008	0.917	1.174	0.764	0.974	0.792	0.957	0.863	0.855	1.038	0.965	0.964	1.084
Pyroglutamic acid	0.47	0.914	0.395	0.797	0.253	0.647	0.516	0.876	0.899	1.306	1.308	1.316	0.486	0.8	0.446	0.461	0.874	0.986	1.163	0.873	0.58	0.635	0.456	0.547	0.853	0.847	0.872	0.652	0.454	0.479	0.485	0.537	0.826	0.747	0.599	0.77
S-Adenosylhomocysteine	0.036	0.042	0.027	0.037	0.005	0.033	0.044	0.043	0.054	0.045	0.061	0.049	0.023	0.026	0.005	0.013	0.035	0.089	0.081	0.072	0.014	0.034	0.026	0.029	0.026	0.022	0.028	0.07	0.024	0.025	0.041	0.041	0.036	0.063	0.025	0.039
S-Adenosylmethionine	0.102	0.144	0.091	0.139	0.027	0.084	0.118	0.159	0.179	0.164	0.232	0.247	0.098	0.127	0.049	0.071	0.126	N.D.	0.269	0.27	0.114	0.117	0.129	0.16	0.123	0.157	0.167	0.204	0.164	0.15	0.179	0.206	0.131	0.175	0.14	0.155
Sarcosine	0.057	0.071	0.037	0.062	0.02	0.052	0.051	0.076	0.09	0.12	0.127	0.108	0.045	0.055	0.032	0.034	0.086	0.081	0.113	0.11	0.045	0.055	0.051	0.054	0.091	0.074	0.087	0.108	0.076	0.057	0.072	0.077	0.069	0.073	0.068	0.073
Serine	2.553	3.192	2.159	3.144	1.058	2.702	2.131	2.861	3.595	5.13	4.322	3.972	2.168	2.898	1.836	1.881	2.956	3.999	3.821	4.046	2.258	2.249	2.513	2.334	3.734	3.12	3.376	7.227	3.439	2.521	2.954	3.024	3.009	3.164	3.057	2.93
Serotonin	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Spermidine	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Spermine	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Stearoylcarnitine	0	0.001	0	0.001	0	0.001	0	0.001	7.66E-05	0.001	0	0.001	0	0.001	0	0.001	7.46E-05	0.001	0	0.001	0	0.001	0	0.001	0	0.001	0	0.001	0.001	0.002	0	0.001	0.001	0.001	0.001	0.001
Sucrose	0.026	0.404	0.043	0.391	0.062	0.419	0.027	0.327	0.03	0.467	0.013	0.261	0.05	0.397	0.048	0.472	0.033	0.46	0.014	0.21	0.041	0.316	0.027	0.308	0.025	0.528	0.027	0.479	0.019	0.362	0.028	0.295	0.19	0.151	0.174	0.154
Taurine	5.83	3.235	4.821	3.69	6.973	4.515	6.562	4.913	1.259	1.008	1.722	1.63	1.379	1.361	2.082	1.316	2.54	2.868	2.605	3.688	3.842	4.139	5.754	2.975	1.077	1.288	1.155	1.307	2.098	1.75	2.03	3.04	1.841	2.019	2.092	2.15
Tetrahydrocurcumin	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Theobromine	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Theophylline	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Thiamine	0.244	0.329	0.295	0.4	0.111	0.223	0.244	0.452	0.436	0.485	0.528	0.583	0.299	0.243	0.194	0.215	0.392	0.349	0.743	0.571	0.304	0.29	0.308	0.309	0.293	0.318	0.44	0.318	0.276	0.244	0.344	0.256	0.353	0.361	0.402	0.365
Threonine	4.419	5.336	3.085	4.488	1.093	2.766	3.338	4.424	6.503	8.101	7.858	7.308	2.944	3.465	1.917	2.145	5.47	6.322	7.846	8.689	3.171	2.973	3.071	3.617	5.105	4.331	4.177	5.568	3.257	3.425	3.796	4.105	4.437	4.155	4.262	5.818
Trigonelline	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Trimethylamine-N-oxide	0.014	0.013	0.011	0.012	0.01	0.01	0.012	0.012	0.017	0.016	0.016	0.016	0.013	0.014	0.013	0.012	0.015	0.016	0.017	0.017	0.013	0.013	0.012	0.011	0.011	0.01	0.01	0.011	0.01	0.008	0.01	0.009	0.019	0.02	0.021	0.019
Trimethylamine-d9 N-oxide	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Tryptophan	0.041	0.053	0.03	0.046	0.019	0.038	0.041	0.057	0.039	0.033	0.067	0.073	0.033	0.041	0.028	0.025	0.043	0.048	0.068	0.077	0.041	0.044	0.045	0.046	0.027	0.035	0.031	0.036	0.044	0.035	0.043	0.05	0.046	0.045	0.055	0.047
Tyrosine	0.008	0.01	0.005	0.009	0.002	0.006	0.007	0.011	0.012	0.019	0.017	0.019	0.006	0.008	0.004	0.005	0.01	0.016	0.017	0.018	0.006	0.007	0.008	0.008	0.01	0.009	0.01	0.01	0.008	0.007	0.008	0.01	0.009	0.009	0.01	0.01
Uracil	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Ureidopropionic acid	0.007	0.009	0.003	0.003	0	0.002	0.005	0.01	0.01	0.014	0.015	0.013	0.004	0.001	0	0.001	0.009	0.008	0.014	0.009	0.003	0.003	0.002	0.003	0.007	0.007	0.003	0.005	0.006	0.002	0.004	0.007	0.004	0.006	0.004	0.007
Uric acid	0.062	0.095	0.087	0.096	0.069	0.072	0.038	0.062	0.059	0.247	0.141	0.094	0.082	0.164	0.099	0.072	0.111	0.067	0.076	0.063	0.087	0.059	0.112	0.064	0.069	0.098	0.093	N.D.	0.056	0.073	0.055	0.078	0.075	0.099	0.086	0.071
Uridine	0.004	N.D.	0.002	0.002	0.001	0.003	0.002	0.002	N.D.	0.001	0.003	0.002	0.003	0.003	0.002	0.001	0.001	0.002	0.005	0.006	0.004	0.004	0.003	0.002	0.004	0.002	0.006	0.002	0.005	0.002	0.004	0.002	0.001	0.003	0.002	0.001
Valerobetaine	0.032	0.034	0.025	0.035	0.007	0.022	0.031	0.037	0.042	0.046	0.078	0.076	0.025	0.028	0.01	0.013	0.028	0.034	0.079	0.081	0.024	0.026	0.028	0.03	0.032	0.027	0.04	0.047	0.036	0.03	0.033	0.037	0.034	0.033	0.033	0.037
Valerobetaine-d9	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Valine	0.007	0.005	0.003	0.004	0.002	0.004	0.005	0.006	0.002	0.003	0.004	0.004	0.001	0.002	0.001	0.001	0.004	0.007	0.006	0.008	0.003	0.004	0.005	0.003	0.002	0.001	0.002	0.002	0.002	0.002	0.002	0.004	0.002	0.003	0.002	0.003
Valine-d8	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Xanthine	0.003	0.004	0.003	0.004	0.003	0.003	0.003	0.004	0.004	0.005	0.004	0.004	0.003	0.003	0.003	0.004	0.004	0.005	0.003	0.004	0.003	0.003	0.003	0.003	0.005	0.007	0.004	0.007	0.003	0.003	0.003	0.004	0.006	0.006	0.006	0.007
Xanthosine	0.01	0.011	0.01	0.01	0.012	0.009	0.007	0.003	0.011	0.011	0.007	0.006	0.009	0.009	0.011	0.015	0.011	0.008	0.005	0.005	0.007	0.006	0.006	0.006	0.017	0.021	0.011	0.016	0.008	0.008	0.008	0.007	0.014	0.014	0.015	0.015
alpha-Tocopherol	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
cADPRibose	0.007	0.01	0.004	0.006	0.001	0.003	0.006	0.007	0.01	0.01	0.011	0.013	0.004	0.006	0.002	0.002	0.008	N.D.	0.018	0.017	0.004	0.006	0.006	0.007	0.009	0.007	0.01	0.011	0.01	0.008	0.008	0.011	0.007	0.01	0.008	0.008
gamma-Aminobutyric acid	0.001	0.001	0	0	7.39E-05	0	0	0	0.001	0.001	0.001	0.001	0	0	9.03E-05	0	0.001	0.001	0.001	0.001	0	0	0	0	0.001	0.001	0	0.001	0	0	0	0	0.001	0.001	0.001	0.001
MS_METABOLITE_DATA_END
#METABOLITES
METABOLITES_START
metabolite_name	Expected RT	MS1_mz	MS2_mz
1-Methylhistidine	6.35	170	124
1-Methylnicotinamide	4.34	137	94
1-Methylxanthine	2.9	167	110
1-Methylxanthosine	2.79	299	167.1
1,3-15N2-Uric acid	5.17	171.1	143
13C10,15N5-Adenosine	3.88	283	146.1
13C3-Thiamine	5.12	268	122.1
13C5-Hypoxanthine	3.74	142.2	124
13C5-Xanthosine	4.56	290	153
15N4-Inosine	4.35	273.1	141
2-Aminobenzoic acid	1.05	138.1	92
3-Hydroxy-N6,N6,N6-Trimethyllysine	6.77	205.2	128.1
3-Hydroxybutyric acid	1.3	105	87
3-Hydroxykynurenine	0.95	225.1	179.1
3-Hydroxytyrosol	3.74	137.1	119
3-Methoxytyrosine	5.32	212.1	166.1
3-Methylxanthine	3.1	167	69
3-Methylxanthosine	2.98	299.1	167.1
4-Hydroxyproline	5.9	132	68.1
4-Trimethylammoniobutanal	6.86	130.1	71.1
5-Glutamylalanine	7	219.1	90.1
5-Hydroxyindoleacetic acid	1.6	192.1	146.1
5-Hydroxytryptophan	4.9	221.1	175
5'-Methylthioadenosine	2.83	298	136
6-Aminouracil	4.3	128	85
6C13-Phenylalanine	4.92	172.1	126.1
6C13-Tyrosine	5.42	188.1	142.1
7-alpha-Hydroxy-3-oxo-4-cholestenoate	1.52	431.3	395.3
7-Methylguanine	4.32	166.1	124.1
7-Methylguanosine	5.48	298	166
7-Methylxanthine	3.3	167.1	69
7-Methylxanthosine	3.2	299.2	167.1
8-Hydroxy-deoxyguanosine	5.18	284.1	140
8-Oxo-deoxyguanosine-13C,15N2	4.8	300	168
8-Hydroxyguanosine	4.76	287.3	171
9-Hexadecenoylcarnitine	3.12	398.3	85
Acetylcarnitine	4.2	204.1	85
Acetylcarnitine-d3	4.24	207.1	85
Adenine	4.05	136.1	119
Adenosine	3.87	268.2	136.1
Adenosine diphosphate ribose	7.33	560.1	136.1
Adenosylcobalamin	6	801.5	676.3
AICA-riboside	4.43	259	110
Alanine	5.87	90.1	44.2
Alanine-d4	5.82	94.1	48.1
alpha-Tocopherol	1	431.4	165.1
Arabitol	4.7	153.1	153.1
Arginine	6.86	175.1	70
Arginine-d4C13	6.85	180.1	75
Ascorbate	3.8	177	95
Asparagine	6.21	133.1	74
Aspartic acid	6.33	134	74
Aspartic acid-d3	6.46	137	75
Asymmetric dimethylarginine	6.44	203.2	70.3
Betaine	5.17	118.1	59.1
Betaine-d11	4.94	129.1	68.1
Bilirubin	1.01	585.3	299.1
Butyrobetaine	4.3	146.1	87
Butyrylcarnitine	3.68	232.2	85
Butyrylcarnitine-d3	3.83	235.2	85
Cadaverine	7.21	102.9	86.1
cADPRibose	7.14	542.1	136.1
Caffeine	1.26	195.1	138
Capsaicin	1.05	306.2	137.1
Carnitine	4.99	162.1	103.1
Carnitine-d9	4.99	171.2	85
Choline	3.97	104	60
Citrulline	6.36	176.1	113.1
Citrulline-d2	6.24	178.1	115.1
Cotinine	1.77	177.1	80
Creatine	5.76	132	90
Creatinine	4.27	114	44.1
Creatinine-d3	4.24	117	86
Curcumin	1.01	369	177
Cyanocobalamin	6.09	678.5	997.5
Cystathionine	7	223.1	134
Cysteine	5.65	122	76
Cysteinylglycine	6.86	179.1	162.2
Cystine	7.25	241	74
Cytosine	4.94	112.1	94.9
Decanoylcarnitine	3.33	316.2	85
Demethoxycurcumin	1.31	339.1	255.2
Deoxyadenosine	3.67	252.1	136
Deoxyguanosine	4.67	268.1	152
Deoxyinosine	3.6	253	137
Dimethylethanolamine	4.25	90	72
Dimethylglycine	5.41	104	58
DNL201	1.08	340.3	313.2
Dodecanoylcarnitine	3.85	343.3	85
Dopa	5.62	198	152
Dopamine	4.54	154	137
Dopamine 3-o-sulfate	3.7	234	137.1
Dopamine 4-o-sulfate	3.9	234.1	137.2
Epinephrine	4.73	166	107
Ergothioneine	5.71	230.1	127
Ethanolamine	5.23	62	44.2
Formylanthranilic acid	1.3	166	120
gamma-Aminobutyric acid	5.08	104.1	87
Gamma-Glutamylcysteine	6.46	251.1	122
Glucosamine	6.45	180	162
Glucose	5.31	202.8	202.8
Glutamic acid-d3	5.97	151	133.1
Glutamic acid	6.07	148.1	84
Glutamine	6.22	147.1	84
Glutathione	6.11	308.1	179.1
Glutathione disulfide	7.48	613.2	355.1
Glutathione-(glycine-13C2,15N)	6.1	311.3	182.1
Glycerophosphocholine	6.24	258.1	104
Glycine	5.92	76	30
Guanine	4.72	152.2	110
Guanosine	5.09	284.1	152
Hexanoylcarnitine	3.46	260.2	85
Histamine	6.5	112.1	95
Histidine	6.93	156.1	110.1
Homocysteine	5.05	136.1	90.1
Homoserine	5.88	120.2	56.2
Hydroxybutyrylcarnitine	4.65	248.2	85
Hydroxyhexanoylcarnitine	4.71	276.2	85
Hydroxyisovaleroyl carnitine	4.3	262.2	85
Hydroxypropionylcarnitine	4.9	234.1	85
Hypoxanthine	3.75	137.2	118.8
Imidazoleacetic acid	5.18	127	81
Indole	1	118	91
Inosine	4.33	269.1	137
Isovalerylcarnitine	3.56	246.2	85
Isovalerylcarnitine-d9	3.55	255.2	85
Kynurenic acid	4.27	190.1	116
Kynurenine	4.81	209	146
Leucine	4.94	132.1	86
Leucine-d3	4.74	135.1	89.1
Linoleyl carnitine	3.05	424.3	85
LPC(18:1(d7))	3.85	529.3	184.1
Lysine	6.93	147.1	84
Mannitol	5.44	183	69
Mannose	5.5	202.8	202.8
Melatonin	3.67	233.1	174.1
Methionine	5.04	150.1	104.1
Methionine S-oxide	6.05	166.1	74
Methionine-d3	5.23	153.1	107
Methylcobalamin	6.04	673	971.5
Myristoylcarnitine	2.9	372	85
Myristoylcarnitine-d9	3.15	381.4	85
N-Acetylalanine	1.8	160.1	72.1
N-Acetylaspartic acid	3.42	176.1	176.1
N-Acetylcysteine	1.3	163	121.2
N-Acetylglutamic acid	3.49	189	130
N-Acetylneuraminic acid	6.69	310.1	274
N-Acetylputrescine	4.89	131.1	114
N-Acetylserine	3.38	148	106
N-Alpha-acetyllysine	5.69	189	84
N,N'-Bis(gamma-glutamyl)cystine	7	499.1	453.1
N1-Acetylspermidine	6.4	188.2	171
N1-Acetylspermidine-d6	6.5	194.3	106.2
N1-Acetylspermine	7.7	245.2	100.1
N1-N12-Diacetylspermine	6.1	287.2	100.1
N1-N8-Diacetylspermidine	4.93	230.5	100.2
N1-N8-Diacetylspermidine-d6	4.93	236.2	103.2
N15C13-Glycine	5.92	78	32
N6,N6,N6-Trimethyllysine	6.62	189.2	84.1
NAD	7.13	664.1	428.2
Niacinamide	1.86	123.1	80
Niacinamide-d4	1.88	127	84
Nicotinamide mononucleotide	6.6	335.2	123.1
Nicotinamide riboside	5.48	255	123
Nicotinic acid	1.87	124.1	78
Nitrotyrosine	4.78	227.1	210
Nonanoylcarnitine	3.3	302.2	85
Norepinephrine	5.1	152	107
Octanoylcarnitine	3.31	288.2	85
Octanoylcarnitine-d3	3.44	291.2	85
Ornithine	6.98	133	70
Ornithine-d2	6.98	135.1	117.2
Palmitoylcarnitine	3	400	85
Palmitoylcarnitine-d3	3.08	403.4	85
Paraxanthine	1.85	181.1	124
Phenylalanine	4.91	166.1	120.1
Piperanine	1.34	288.2	135.2
Piperine	1.04	286.1	201.1
Pipernonaline	1.3	342.1	229.2
Piperyline	1.39	272.2	201.1
Proline	5.27	116	70.1
Propionylcarnitine	3.84	218.1	85
Propionylcartinine-d3	4	221.2	85
Putrescine	6.3	89	72
Pyridoxamine	6.06	169	152
Pyroglutamic acid	3.29	130	84
S-Adenosylhomocysteine	6.23	385.1	136
S-Adenosylmethionine	7.13	399	250
Sarcosine	5.75	90	44.1
Serine	6.14	106	60
Serotonin	4.42	177	160
Spermidine	7.8	146.2	72
Spermine	4.95	203.1	129.1
Stearoylcarnitine	3.02	428.4	85
Sucrose	6	364.8	202.8
Taurine	5.37	126	80.1
Tetrahydrocurcumin	1.38	355	137.1
Theobromine	2.1	181	138.3
Theophylline	1.7	181.1	124
Thiamine	5.11	265	122
Threonine	5.87	120	74
Trigonelline	5.3	138.1	94.1
Trimethylamine-d9 N-oxide	4.17	85	66
Trimethylamine-N-oxide	4.03	76.1	58.1
Tryptophan	4.75	205	146.1
Tyrosine	5.26	182.1	136
Uracil	2.47	113	70
Ureidopropionic acid	3.24	133	115
Uric acid	5.28	169.1	141
Uridine	3.91	245	113
Valerobetaine	4.09	160	101.1
Valerobetaine-d9	4.09	169.1	101.1
Valine	5.18	118.1	55
Valine-d8	5.32	126	80.1
Xanthine	4.05	153	110
Xanthosine	4.71	285	153
METABOLITES_END
#END