#METABOLOMICS WORKBENCH Jung_DNLI_20250918_120528 DATATRACK_ID:6449 STUDY_ID:ST004230 ANALYSIS_ID:AN007041 PROJECT_ID:PR002147
VERSION             	1
CREATED_ON             	September 25, 2025, 10:37 am
#PROJECT
PR:PROJECT_TITLE                 	TREM2 expression level is critical for microglial state, metabolic capacity and
PR:PROJECT_TITLE                 	efficacy of TREM2 agonism
PR:PROJECT_TYPE                  	Preclinical Mouse study
PR:PROJECT_SUMMARY               	Triggering receptor expressed on myeloid cells 2 (TREM2) is a central regulator
PR:PROJECT_SUMMARY               	of microglial activity and sequence variants are major risk factors for late
PR:PROJECT_SUMMARY               	onset Alzheimer’s disease (LOAD). To better understand the molecular and
PR:PROJECT_SUMMARY               	functional changes associated with TREM2 signalling, we generated a TREM2
PR:PROJECT_SUMMARY               	reporter mouse model and observed a gradual upregulation of reporter expression
PR:PROJECT_SUMMARY               	with increasing plaque proximity. Isolated microglia were sorted based on
PR:PROJECT_SUMMARY               	reporter expression and their transcriptomic profiles acquired in both wildtype
PR:PROJECT_SUMMARY               	and APP transgenic animals, allowing us to disentangle TREM2 versus
PR:PROJECT_SUMMARY               	pathology-specific effects. Bulk RNA sequencing highlighted TREM2
PR:PROJECT_SUMMARY               	level-dependent changes in major immunometabolic pathways, with enrichment of
PR:PROJECT_SUMMARY               	genes in oxidative phosphorylation and cholesterol metabolism in microglia with
PR:PROJECT_SUMMARY               	increased TREM2 expression. To confirm these findings, we next analysed uptake
PR:PROJECT_SUMMARY               	of fluorodeoxyglucose (FDG) and examined metabolomic and lipidomic profiles.
PR:PROJECT_SUMMARY               	Again, independent of Aβ pathology, TREM2 expression correlated with uptake of
PR:PROJECT_SUMMARY               	FDG as well as increased cellular redox, energetics, and cholesterol
PR:PROJECT_SUMMARY               	homeostasis. Finally, we performed chronic treatment with a brain penetrant
PR:PROJECT_SUMMARY               	TREM2 agonist and identified a window of TREM2 expression where microglia are
PR:PROJECT_SUMMARY               	most responsive. Thus, our data provide novel insights into TREM2-mediated
PR:PROJECT_SUMMARY               	regulation of microglial metabolic function and informs current efforts to bring
PR:PROJECT_SUMMARY               	TREM2 agonists into clinical application.
PR:INSTITUTE                     	Denali Therapeutics
PR:DEPARTMENT                    	Development Sciences
PR:LAST_NAME                     	Suh
PR:FIRST_NAME                    	Jung
PR:ADDRESS                       	161 Oyster Point Blvd, South San Francisco, California, 94080, USA
PR:EMAIL                         	suh@dnli.com
PR:PHONE                         	+1 6507973837
PR:FUNDING_SOURCE                	Denali Therapeutics
PR:PUBLICATIONS                  	https://www.biorxiv.org/content/10.1101/2024.07.18.604115v1
#STUDY
ST:STUDY_TITLE                   	Effect of ATV:4D9 on Microglial Metabolism and FDG levels in mKate-TREM2/APP
ST:STUDY_TITLE                   	mouse model
ST:STUDY_SUMMARY                 	Targeted lipid and metabolite profiling was performed in microglia from
ST:STUDY_SUMMARY                 	mKate-TREM2/APP mice treated with ATV:4D9, a blood–brain barrier-penetrant
ST:STUDY_SUMMARY                 	TREM2 agonist antibody, following FDG injection to assess effects on glucose
ST:STUDY_SUMMARY                 	uptake. Microglia were isolated and sorted by fluorescent TREM2 reporter
ST:STUDY_SUMMARY                 	expression, enabling analysis of low-, mid-, and high-TREM2 subpopulations.
ST:STUDY_SUMMARY                 	LC-MS data show that the low TREM2 subpopulations derived from mice dosed
ST:STUDY_SUMMARY                 	chronically with ATV:4D9 and isotype control have similar lipidomic and
ST:STUDY_SUMMARY                 	metabolomic profiles, while the mid and high TREM2 subpopulations are distinct
ST:STUDY_SUMMARY                 	and cluster depending upon antibody dosing, suggesting that the TREM2 agonist
ST:STUDY_SUMMARY                 	antibody differentially modulates microglial metabolism dependent on TREM2
ST:STUDY_SUMMARY                 	expression level.
ST:INSTITUTE                     	Denali Therapeutics
ST:LAST_NAME                     	Suh
ST:FIRST_NAME                    	Jung
ST:ADDRESS                       	161 Oyster Point Blvd, South San Francisco, California, 94080, USA
ST:EMAIL                         	suh@dnli.com
ST:PHONE                         	+1 6507973837
ST:NUM_GROUPS                    	5
ST:TOTAL_SUBJECTS                	26
ST:NUM_MALES                     	26
ST:STUDY_COMMENTS                	Release as soon as it is possible
ST:PUBLICATIONS                  	https://www.biorxiv.org/content/10.1101/2024.07.18.604115v1
#SUBJECT
SU:SUBJECT_TYPE                  	Mammal
SU:SUBJECT_SPECIES               	Mus musculus
SU:TAXONOMY_ID                   	10090
SU:AGE_OR_AGE_RANGE              	15 mo
SU:GENDER                        	Male
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data
SUBJECT_SAMPLE_FACTORS           	38_blank	HSA-000036741	condition:NA | treatment:NA | Sample source:microglia	genotype=APP; RAW_FILE_NAME(Raw Source Name.LN)=20240223_BL_HEX-000754_Lipid_Neg_001; RAW_FILE_NAME(Raw Source Name.LP)=20240223_BL_HEX-000754_Lipid_Pos_001; RAW_FILE_NAME(Raw Source Name.MN)=20240225_BL_HEX-000754_Met_Neg_001; RAW_FILE_NAME(Raw Source Name.MP)=20240223_BL_HEX-000754_Met_Pos_001; RAW_FILE_NAME(Raw Source Name.FN)=20240225_BL_HEX-000754_pFDG_Neg_001
SUBJECT_SAMPLE_FACTORS           	Pool_1	HSA-000036740	condition:NA | treatment:NA | Sample source:microglia	genotype=APP; RAW_FILE_NAME(Raw Source Name.LN)=20240223_BL_HEX-000754_Lipid_Neg_002; RAW_FILE_NAME(Raw Source Name.LP)=20240223_BL_HEX-000754_Lipid_Pos_002; RAW_FILE_NAME(Raw Source Name.MN)=20240225_BL_HEX-000754_Met_Neg_002; RAW_FILE_NAME(Raw Source Name.MP)=20240223_BL_HEX-000754_Met_Pos_002; RAW_FILE_NAME(Raw Source Name.FN)=20240225_BL_HEX-000754_pFDG_Neg_002
SUBJECT_SAMPLE_FACTORS           	15_53_ATV Iso _mid	HSA-000036718	condition:mid | treatment:ATV Iso | Sample source:microglia	genotype=APP; RAW_FILE_NAME(Raw Source Name.LN)=20240223_BL_HEX-000754_Lipid_Neg_003; RAW_FILE_NAME(Raw Source Name.LP)=20240223_BL_HEX-000754_Lipid_Pos_003; RAW_FILE_NAME(Raw Source Name.MN)=20240225_BL_HEX-000754_Met_Neg_003; RAW_FILE_NAME(Raw Source Name.MP)=20240223_BL_HEX-000754_Met_Pos_003; RAW_FILE_NAME(Raw Source Name.FN)=20240225_BL_HEX-000754_pFDG_Neg_003
SUBJECT_SAMPLE_FACTORS           	13_51_ATV Iso _mid	HSA-000036716	condition:mid | treatment:ATV Iso | Sample source:microglia	genotype=APP; RAW_FILE_NAME(Raw Source Name.LN)=20240223_BL_HEX-000754_Lipid_Neg_004; RAW_FILE_NAME(Raw Source Name.LP)=20240223_BL_HEX-000754_Lipid_Pos_004; RAW_FILE_NAME(Raw Source Name.MN)=20240225_BL_HEX-000754_Met_Neg_004; RAW_FILE_NAME(Raw Source Name.MP)=20240223_BL_HEX-000754_Met_Pos_004; RAW_FILE_NAME(Raw Source Name.FN)=20240225_BL_HEX-000754_pFDG_Neg_004
SUBJECT_SAMPLE_FACTORS           	27_53_ATV Iso _high	HSA-000036730	condition:high | treatment:ATV Iso | Sample source:microglia	genotype=APP; RAW_FILE_NAME(Raw Source Name.LN)=20240223_BL_HEX-000754_Lipid_Neg_005; RAW_FILE_NAME(Raw Source Name.LP)=20240223_BL_HEX-000754_Lipid_Pos_005; RAW_FILE_NAME(Raw Source Name.MN)=20240225_BL_HEX-000754_Met_Neg_005; RAW_FILE_NAME(Raw Source Name.MP)=20240223_BL_HEX-000754_Met_Pos_005; RAW_FILE_NAME(Raw Source Name.FN)=20240225_BL_HEX-000754_pFDG_Neg_005
SUBJECT_SAMPLE_FACTORS           	3_53_ATV Iso _low	HSA-000036706	condition:low | treatment:ATV Iso | Sample source:microglia	genotype=APP; RAW_FILE_NAME(Raw Source Name.LN)=20240223_BL_HEX-000754_Lipid_Neg_006; RAW_FILE_NAME(Raw Source Name.LP)=20240223_BL_HEX-000754_Lipid_Pos_006; RAW_FILE_NAME(Raw Source Name.MN)=20240225_BL_HEX-000754_Met_Neg_006; RAW_FILE_NAME(Raw Source Name.MP)=20240223_BL_HEX-000754_Met_Pos_006; RAW_FILE_NAME(Raw Source Name.FN)=20240225_BL_HEX-000754_pFDG_Neg_006
SUBJECT_SAMPLE_FACTORS           	33_66_ATV Iso _high	HSA-000036736	condition:high | treatment:ATV Iso | Sample source:microglia	genotype=APP; RAW_FILE_NAME(Raw Source Name.LN)=20240223_BL_HEX-000754_Lipid_Neg_007; RAW_FILE_NAME(Raw Source Name.LP)=20240223_BL_HEX-000754_Lipid_Pos_007; RAW_FILE_NAME(Raw Source Name.MN)=20240225_BL_HEX-000754_Met_Neg_007; RAW_FILE_NAME(Raw Source Name.MP)=20240223_BL_HEX-000754_Met_Pos_007; RAW_FILE_NAME(Raw Source Name.FN)=20240225_BL_HEX-000754_pFDG_Neg_007
SUBJECT_SAMPLE_FACTORS           	31_59_ATV 4D9 _high	HSA-000036734	condition:high | treatment:ATV 4D9 | Sample source:microglia	genotype=APP; RAW_FILE_NAME(Raw Source Name.LN)=20240223_BL_HEX-000754_Lipid_Neg_008; RAW_FILE_NAME(Raw Source Name.LP)=20240223_BL_HEX-000754_Lipid_Pos_008; RAW_FILE_NAME(Raw Source Name.MN)=20240225_BL_HEX-000754_Met_Neg_008; RAW_FILE_NAME(Raw Source Name.MP)=20240223_BL_HEX-000754_Met_Pos_008; RAW_FILE_NAME(Raw Source Name.FN)=20240225_BL_HEX-000754_pFDG_Neg_008
SUBJECT_SAMPLE_FACTORS           	18_56_ATV 4D9 _mid	HSA-000036721	condition:mid | treatment:ATV 4D9 | Sample source:microglia	genotype=APP; RAW_FILE_NAME(Raw Source Name.LN)=20240223_BL_HEX-000754_Lipid_Neg_009; RAW_FILE_NAME(Raw Source Name.LP)=20240223_BL_HEX-000754_Lipid_Pos_009; RAW_FILE_NAME(Raw Source Name.MN)=20240225_BL_HEX-000754_Met_Neg_009; RAW_FILE_NAME(Raw Source Name.MP)=20240223_BL_HEX-000754_Met_Pos_009; RAW_FILE_NAME(Raw Source Name.FN)=20240225_BL_HEX-000754_pFDG_Neg_009
SUBJECT_SAMPLE_FACTORS           	34_68_ATV Iso _high	HSA-000036737	condition:high | treatment:ATV Iso | Sample source:microglia	genotype=APP; RAW_FILE_NAME(Raw Source Name.LN)=20240223_BL_HEX-000754_Lipid_Neg_010; RAW_FILE_NAME(Raw Source Name.LP)=20240223_BL_HEX-000754_Lipid_Pos_010; RAW_FILE_NAME(Raw Source Name.MN)=20240225_BL_HEX-000754_Met_Neg_010; RAW_FILE_NAME(Raw Source Name.MP)=20240223_BL_HEX-000754_Met_Pos_010; RAW_FILE_NAME(Raw Source Name.FN)=20240225_BL_HEX-000754_pFDG_Neg_010
SUBJECT_SAMPLE_FACTORS           	6_56_ATV 4D9 _low	HSA-000036709	condition:low | treatment:ATV 4D9 | Sample source:microglia	genotype=APP; RAW_FILE_NAME(Raw Source Name.LN)=20240223_BL_HEX-000754_Lipid_Neg_011; RAW_FILE_NAME(Raw Source Name.LP)=20240223_BL_HEX-000754_Lipid_Pos_011; RAW_FILE_NAME(Raw Source Name.MN)=20240225_BL_HEX-000754_Met_Neg_011; RAW_FILE_NAME(Raw Source Name.MP)=20240223_BL_HEX-000754_Met_Pos_011; RAW_FILE_NAME(Raw Source Name.FN)=20240225_BL_HEX-000754_pFDG_Neg_011
SUBJECT_SAMPLE_FACTORS           	36_70_ATV Iso _high	HSA-000036739	condition:high | treatment:ATV Iso | Sample source:microglia	genotype=APP; RAW_FILE_NAME(Raw Source Name.LN)=20240223_BL_HEX-000754_Lipid_Neg_012; RAW_FILE_NAME(Raw Source Name.LP)=20240223_BL_HEX-000754_Lipid_Pos_012; RAW_FILE_NAME(Raw Source Name.MN)=20240225_BL_HEX-000754_Met_Neg_012; RAW_FILE_NAME(Raw Source Name.MP)=20240223_BL_HEX-000754_Met_Pos_012; RAW_FILE_NAME(Raw Source Name.FN)=20240225_BL_HEX-000754_pFDG_Neg_012
SUBJECT_SAMPLE_FACTORS           	16_54_ATV 4D9 _mid	HSA-000036719	condition:mid | treatment:ATV 4D9 | Sample source:microglia	genotype=APP; RAW_FILE_NAME(Raw Source Name.LN)=20240223_BL_HEX-000754_Lipid_Neg_013; RAW_FILE_NAME(Raw Source Name.LP)=20240223_BL_HEX-000754_Lipid_Pos_013; RAW_FILE_NAME(Raw Source Name.MN)=20240225_BL_HEX-000754_Met_Neg_013; RAW_FILE_NAME(Raw Source Name.MP)=20240223_BL_HEX-000754_Met_Pos_013; RAW_FILE_NAME(Raw Source Name.FN)=20240225_BL_HEX-000754_pFDG_Neg_013
SUBJECT_SAMPLE_FACTORS           	25_51_ATV Iso _high	HSA-000036728	condition:high | treatment:ATV Iso | Sample source:microglia	genotype=APP; RAW_FILE_NAME(Raw Source Name.LN)=20240223_BL_HEX-000754_Lipid_Neg_014; RAW_FILE_NAME(Raw Source Name.LP)=20240223_BL_HEX-000754_Lipid_Pos_014; RAW_FILE_NAME(Raw Source Name.MN)=20240225_BL_HEX-000754_Met_Neg_014; RAW_FILE_NAME(Raw Source Name.MP)=20240223_BL_HEX-000754_Met_Pos_014; RAW_FILE_NAME(Raw Source Name.FN)=20240225_BL_HEX-000754_pFDG_Neg_014
SUBJECT_SAMPLE_FACTORS           	9_66_ATV Iso _low	HSA-000036712	condition:low | treatment:ATV Iso | Sample source:microglia	genotype=APP; RAW_FILE_NAME(Raw Source Name.LN)=20240223_BL_HEX-000754_Lipid_Neg_015; RAW_FILE_NAME(Raw Source Name.LP)=20240223_BL_HEX-000754_Lipid_Pos_015; RAW_FILE_NAME(Raw Source Name.MN)=20240225_BL_HEX-000754_Met_Neg_015; RAW_FILE_NAME(Raw Source Name.MP)=20240223_BL_HEX-000754_Met_Pos_015; RAW_FILE_NAME(Raw Source Name.FN)=20240225_BL_HEX-000754_pFDG_Neg_015
SUBJECT_SAMPLE_FACTORS           	7_59_ATV 4D9 _low	HSA-000036710	condition:low | treatment:ATV 4D9 | Sample source:microglia	genotype=APP; RAW_FILE_NAME(Raw Source Name.LN)=20240223_BL_HEX-000754_Lipid_Neg_016; RAW_FILE_NAME(Raw Source Name.LP)=20240223_BL_HEX-000754_Lipid_Pos_016; RAW_FILE_NAME(Raw Source Name.MN)=20240225_BL_HEX-000754_Met_Neg_016; RAW_FILE_NAME(Raw Source Name.MP)=20240223_BL_HEX-000754_Met_Pos_016; RAW_FILE_NAME(Raw Source Name.FN)=20240225_BL_HEX-000754_pFDG_Neg_016
SUBJECT_SAMPLE_FACTORS           	19_59_ATV 4D9 _mid	HSA-000036722	condition:mid | treatment:ATV 4D9 | Sample source:microglia	genotype=APP; RAW_FILE_NAME(Raw Source Name.LN)=20240223_BL_HEX-000754_Lipid_Neg_017; RAW_FILE_NAME(Raw Source Name.LP)=20240223_BL_HEX-000754_Lipid_Pos_017; RAW_FILE_NAME(Raw Source Name.MN)=20240225_BL_HEX-000754_Met_Neg_017; RAW_FILE_NAME(Raw Source Name.MP)=20240223_BL_HEX-000754_Met_Pos_017; RAW_FILE_NAME(Raw Source Name.FN)=20240225_BL_HEX-000754_pFDG_Neg_017
SUBJECT_SAMPLE_FACTORS           	8_65_ATV 4D9 _low	HSA-000036711	condition:low | treatment:ATV 4D9 | Sample source:microglia	genotype=APP; RAW_FILE_NAME(Raw Source Name.LN)=20240223_BL_HEX-000754_Lipid_Neg_018; RAW_FILE_NAME(Raw Source Name.LP)=20240223_BL_HEX-000754_Lipid_Pos_018; RAW_FILE_NAME(Raw Source Name.MN)=20240225_BL_HEX-000754_Met_Neg_018; RAW_FILE_NAME(Raw Source Name.MP)=20240223_BL_HEX-000754_Met_Pos_018; RAW_FILE_NAME(Raw Source Name.FN)=20240225_BL_HEX-000754_pFDG_Neg_018
SUBJECT_SAMPLE_FACTORS           	2_52_ATV 4D9 _low	HSA-000036705	condition:low | treatment:ATV 4D9 | Sample source:microglia	genotype=APP; RAW_FILE_NAME(Raw Source Name.LN)=20240223_BL_HEX-000754_Lipid_Neg_019; RAW_FILE_NAME(Raw Source Name.LP)=20240223_BL_HEX-000754_Lipid_Pos_019; RAW_FILE_NAME(Raw Source Name.MN)=20240225_BL_HEX-000754_Met_Neg_019; RAW_FILE_NAME(Raw Source Name.MP)=20240223_BL_HEX-000754_Met_Pos_019; RAW_FILE_NAME(Raw Source Name.FN)=20240225_BL_HEX-000754_pFDG_Neg_019
SUBJECT_SAMPLE_FACTORS           	Pool_2	HSA-000037077	condition:NA | treatment:NA | Sample source:microglia	genotype=APP; RAW_FILE_NAME(Raw Source Name.LN)=20240223_BL_HEX-000754_Lipid_Neg_020; RAW_FILE_NAME(Raw Source Name.LP)=20240223_BL_HEX-000754_Lipid_Pos_020; RAW_FILE_NAME(Raw Source Name.MN)=20240225_BL_HEX-000754_Met_Neg_020; RAW_FILE_NAME(Raw Source Name.MP)=20240223_BL_HEX-000754_Met_Pos_020; RAW_FILE_NAME(Raw Source Name.FN)=20240225_BL_HEX-000754_pFDG_Neg_020
SUBJECT_SAMPLE_FACTORS           	12_70_ATV Iso _low	HSA-000036715	condition:low | treatment:ATV Iso | Sample source:microglia	genotype=APP; RAW_FILE_NAME(Raw Source Name.LN)=20240223_BL_HEX-000754_Lipid_Neg_021; RAW_FILE_NAME(Raw Source Name.LP)=20240223_BL_HEX-000754_Lipid_Pos_021; RAW_FILE_NAME(Raw Source Name.MN)=20240225_BL_HEX-000754_Met_Neg_021; RAW_FILE_NAME(Raw Source Name.MP)=20240223_BL_HEX-000754_Met_Pos_021; RAW_FILE_NAME(Raw Source Name.FN)=20240225_BL_HEX-000754_pFDG_Neg_021
SUBJECT_SAMPLE_FACTORS           	29_55_ATV Iso _high	HSA-000036732	condition:high | treatment:ATV Iso | Sample source:microglia	genotype=APP; RAW_FILE_NAME(Raw Source Name.LN)=20240223_BL_HEX-000754_Lipid_Neg_022; RAW_FILE_NAME(Raw Source Name.LP)=20240223_BL_HEX-000754_Lipid_Pos_022; RAW_FILE_NAME(Raw Source Name.MN)=20240225_BL_HEX-000754_Met_Neg_022; RAW_FILE_NAME(Raw Source Name.MP)=20240223_BL_HEX-000754_Met_Pos_022; RAW_FILE_NAME(Raw Source Name.FN)=20240225_BL_HEX-000754_pFDG_Neg_022
SUBJECT_SAMPLE_FACTORS           	23_69_ATV 4D9 _mid	HSA-000036726	condition:mid | treatment:ATV 4D9 | Sample source:microglia	genotype=APP; RAW_FILE_NAME(Raw Source Name.LN)=20240223_BL_HEX-000754_Lipid_Neg_023; RAW_FILE_NAME(Raw Source Name.LP)=20240223_BL_HEX-000754_Lipid_Pos_023; RAW_FILE_NAME(Raw Source Name.MN)=20240225_BL_HEX-000754_Met_Neg_023; RAW_FILE_NAME(Raw Source Name.MP)=20240223_BL_HEX-000754_Met_Pos_023; RAW_FILE_NAME(Raw Source Name.FN)=20240225_BL_HEX-000754_pFDG_Neg_023
SUBJECT_SAMPLE_FACTORS           	20_65_ATV 4D9 _mid	HSA-000036723	condition:mid | treatment:ATV 4D9 | Sample source:microglia	genotype=APP; RAW_FILE_NAME(Raw Source Name.LN)=20240223_BL_HEX-000754_Lipid_Neg_024; RAW_FILE_NAME(Raw Source Name.LP)=20240223_BL_HEX-000754_Lipid_Pos_024; RAW_FILE_NAME(Raw Source Name.MN)=20240225_BL_HEX-000754_Met_Neg_024; RAW_FILE_NAME(Raw Source Name.MP)=20240223_BL_HEX-000754_Met_Pos_024; RAW_FILE_NAME(Raw Source Name.FN)=20240225_BL_HEX-000754_pFDG_Neg_024
SUBJECT_SAMPLE_FACTORS           	21_66_ATV Iso _mid	HSA-000036724	condition:mid | treatment:ATV Iso | Sample source:microglia	genotype=APP; RAW_FILE_NAME(Raw Source Name.LN)=20240223_BL_HEX-000754_Lipid_Neg_025; RAW_FILE_NAME(Raw Source Name.LP)=20240223_BL_HEX-000754_Lipid_Pos_025; RAW_FILE_NAME(Raw Source Name.MN)=20240225_BL_HEX-000754_Met_Neg_025; RAW_FILE_NAME(Raw Source Name.MP)=20240223_BL_HEX-000754_Met_Pos_025; RAW_FILE_NAME(Raw Source Name.FN)=20240225_BL_HEX-000754_pFDG_Neg_025
SUBJECT_SAMPLE_FACTORS           	28_54_ATV 4D9 _high	HSA-000036731	condition:high | treatment:ATV 4D9 | Sample source:microglia	genotype=APP; RAW_FILE_NAME(Raw Source Name.LN)=20240223_BL_HEX-000754_Lipid_Neg_026; RAW_FILE_NAME(Raw Source Name.LP)=20240223_BL_HEX-000754_Lipid_Pos_026; RAW_FILE_NAME(Raw Source Name.MN)=20240225_BL_HEX-000754_Met_Neg_026; RAW_FILE_NAME(Raw Source Name.MP)=20240223_BL_HEX-000754_Met_Pos_026; RAW_FILE_NAME(Raw Source Name.FN)=20240225_BL_HEX-000754_pFDG_Neg_026
SUBJECT_SAMPLE_FACTORS           	4_54_ATV 4D9 _low	HSA-000036707	condition:low | treatment:ATV 4D9 | Sample source:microglia	genotype=APP; RAW_FILE_NAME(Raw Source Name.LN)=20240223_BL_HEX-000754_Lipid_Neg_027; RAW_FILE_NAME(Raw Source Name.LP)=20240223_BL_HEX-000754_Lipid_Pos_027; RAW_FILE_NAME(Raw Source Name.MN)=20240225_BL_HEX-000754_Met_Neg_027; RAW_FILE_NAME(Raw Source Name.MP)=20240223_BL_HEX-000754_Met_Pos_027; RAW_FILE_NAME(Raw Source Name.FN)=20240225_BL_HEX-000754_pFDG_Neg_027
SUBJECT_SAMPLE_FACTORS           	24_70_ATV Iso _mid	HSA-000036727	condition:mid | treatment:ATV Iso | Sample source:microglia	genotype=APP; RAW_FILE_NAME(Raw Source Name.LN)=20240223_BL_HEX-000754_Lipid_Neg_028; RAW_FILE_NAME(Raw Source Name.LP)=20240223_BL_HEX-000754_Lipid_Pos_028; RAW_FILE_NAME(Raw Source Name.MN)=20240225_BL_HEX-000754_Met_Neg_028; RAW_FILE_NAME(Raw Source Name.MP)=20240223_BL_HEX-000754_Met_Pos_028; RAW_FILE_NAME(Raw Source Name.FN)=20240225_BL_HEX-000754_pFDG_Neg_028
SUBJECT_SAMPLE_FACTORS           	35_69_ATV 4D9 _high	HSA-000036738	condition:high | treatment:ATV 4D9 | Sample source:microglia	genotype=APP; RAW_FILE_NAME(Raw Source Name.LN)=20240223_BL_HEX-000754_Lipid_Neg_029; RAW_FILE_NAME(Raw Source Name.LP)=20240223_BL_HEX-000754_Lipid_Pos_029; RAW_FILE_NAME(Raw Source Name.MN)=20240225_BL_HEX-000754_Met_Neg_029; RAW_FILE_NAME(Raw Source Name.MP)=20240223_BL_HEX-000754_Met_Pos_029; RAW_FILE_NAME(Raw Source Name.FN)=20240225_BL_HEX-000754_pFDG_Neg_029
SUBJECT_SAMPLE_FACTORS           	10_68_ATV Iso _low	HSA-000036713	condition:low | treatment:ATV Iso | Sample source:microglia	genotype=APP; RAW_FILE_NAME(Raw Source Name.LN)=20240223_BL_HEX-000754_Lipid_Neg_030; RAW_FILE_NAME(Raw Source Name.LP)=20240223_BL_HEX-000754_Lipid_Pos_030; RAW_FILE_NAME(Raw Source Name.MN)=20240225_BL_HEX-000754_Met_Neg_030; RAW_FILE_NAME(Raw Source Name.MP)=20240223_BL_HEX-000754_Met_Pos_030; RAW_FILE_NAME(Raw Source Name.FN)=20240225_BL_HEX-000754_pFDG_Neg_030
SUBJECT_SAMPLE_FACTORS           	30_56_ATV 4D9 _high	HSA-000036733	condition:high | treatment:ATV 4D9 | Sample source:microglia	genotype=APP; RAW_FILE_NAME(Raw Source Name.LN)=20240223_BL_HEX-000754_Lipid_Neg_031; RAW_FILE_NAME(Raw Source Name.LP)=20240223_BL_HEX-000754_Lipid_Pos_031; RAW_FILE_NAME(Raw Source Name.MN)=20240225_BL_HEX-000754_Met_Neg_031; RAW_FILE_NAME(Raw Source Name.MP)=20240223_BL_HEX-000754_Met_Pos_031; RAW_FILE_NAME(Raw Source Name.FN)=20240225_BL_HEX-000754_pFDG_Neg_031
SUBJECT_SAMPLE_FACTORS           	26_52_ATV 4D9 _high	HSA-000036729	condition:high | treatment:ATV 4D9 | Sample source:microglia	genotype=APP; RAW_FILE_NAME(Raw Source Name.LN)=20240223_BL_HEX-000754_Lipid_Neg_032; RAW_FILE_NAME(Raw Source Name.LP)=20240223_BL_HEX-000754_Lipid_Pos_032; RAW_FILE_NAME(Raw Source Name.MN)=20240225_BL_HEX-000754_Met_Neg_032; RAW_FILE_NAME(Raw Source Name.MP)=20240223_BL_HEX-000754_Met_Pos_032; RAW_FILE_NAME(Raw Source Name.FN)=20240225_BL_HEX-000754_pFDG_Neg_032
SUBJECT_SAMPLE_FACTORS           	11_69_ATV 4D9 _low	HSA-000036714	condition:low | treatment:ATV 4D9 | Sample source:microglia	genotype=APP; RAW_FILE_NAME(Raw Source Name.LN)=20240223_BL_HEX-000754_Lipid_Neg_033; RAW_FILE_NAME(Raw Source Name.LP)=20240223_BL_HEX-000754_Lipid_Pos_033; RAW_FILE_NAME(Raw Source Name.MN)=20240225_BL_HEX-000754_Met_Neg_033; RAW_FILE_NAME(Raw Source Name.MP)=20240223_BL_HEX-000754_Met_Pos_033; RAW_FILE_NAME(Raw Source Name.FN)=20240225_BL_HEX-000754_pFDG_Neg_033
SUBJECT_SAMPLE_FACTORS           	5_55_ATV Iso _low	HSA-000036708	condition:low | treatment:ATV Iso | Sample source:microglia	genotype=APP; RAW_FILE_NAME(Raw Source Name.LN)=20240223_BL_HEX-000754_Lipid_Neg_034; RAW_FILE_NAME(Raw Source Name.LP)=20240223_BL_HEX-000754_Lipid_Pos_034; RAW_FILE_NAME(Raw Source Name.MN)=20240225_BL_HEX-000754_Met_Neg_034; RAW_FILE_NAME(Raw Source Name.MP)=20240223_BL_HEX-000754_Met_Pos_034; RAW_FILE_NAME(Raw Source Name.FN)=20240225_BL_HEX-000754_pFDG_Neg_034
SUBJECT_SAMPLE_FACTORS           	32_65_ATV 4D9 _high	HSA-000036735	condition:high | treatment:ATV 4D9 | Sample source:microglia	genotype=APP; RAW_FILE_NAME(Raw Source Name.LN)=20240223_BL_HEX-000754_Lipid_Neg_035; RAW_FILE_NAME(Raw Source Name.LP)=20240223_BL_HEX-000754_Lipid_Pos_035; RAW_FILE_NAME(Raw Source Name.MN)=20240225_BL_HEX-000754_Met_Neg_035; RAW_FILE_NAME(Raw Source Name.MP)=20240223_BL_HEX-000754_Met_Pos_035; RAW_FILE_NAME(Raw Source Name.FN)=20240225_BL_HEX-000754_pFDG_Neg_035
SUBJECT_SAMPLE_FACTORS           	17_55_ATV Iso _mid	HSA-000036720	condition:mid | treatment:ATV Iso | Sample source:microglia	genotype=APP; RAW_FILE_NAME(Raw Source Name.LN)=20240223_BL_HEX-000754_Lipid_Neg_036; RAW_FILE_NAME(Raw Source Name.LP)=20240223_BL_HEX-000754_Lipid_Pos_036; RAW_FILE_NAME(Raw Source Name.MN)=20240225_BL_HEX-000754_Met_Neg_036; RAW_FILE_NAME(Raw Source Name.MP)=20240223_BL_HEX-000754_Met_Pos_036; RAW_FILE_NAME(Raw Source Name.FN)=20240225_BL_HEX-000754_pFDG_Neg_036
SUBJECT_SAMPLE_FACTORS           	22_68_ATV Iso _mid	HSA-000036725	condition:mid | treatment:ATV Iso | Sample source:microglia	genotype=APP; RAW_FILE_NAME(Raw Source Name.LN)=20240223_BL_HEX-000754_Lipid_Neg_037; RAW_FILE_NAME(Raw Source Name.LP)=20240223_BL_HEX-000754_Lipid_Pos_037; RAW_FILE_NAME(Raw Source Name.MN)=20240225_BL_HEX-000754_Met_Neg_037; RAW_FILE_NAME(Raw Source Name.MP)=20240223_BL_HEX-000754_Met_Pos_037; RAW_FILE_NAME(Raw Source Name.FN)=20240225_BL_HEX-000754_pFDG_Neg_037
SUBJECT_SAMPLE_FACTORS           	1_51_ATV Iso _low	HSA-000036704	condition:low | treatment:ATV Iso | Sample source:microglia	genotype=APP; RAW_FILE_NAME(Raw Source Name.LN)=20240223_BL_HEX-000754_Lipid_Neg_038; RAW_FILE_NAME(Raw Source Name.LP)=20240223_BL_HEX-000754_Lipid_Pos_038; RAW_FILE_NAME(Raw Source Name.MN)=20240225_BL_HEX-000754_Met_Neg_038; RAW_FILE_NAME(Raw Source Name.MP)=20240223_BL_HEX-000754_Met_Pos_038; RAW_FILE_NAME(Raw Source Name.FN)=20240225_BL_HEX-000754_pFDG_Neg_038
SUBJECT_SAMPLE_FACTORS           	14_52_ATV 4D9 _mid	HSA-000036717	condition:mid | treatment:ATV 4D9 | Sample source:microglia	genotype=APP; RAW_FILE_NAME(Raw Source Name.LN)=20240223_BL_HEX-000754_Lipid_Neg_039; RAW_FILE_NAME(Raw Source Name.LP)=20240223_BL_HEX-000754_Lipid_Pos_039; RAW_FILE_NAME(Raw Source Name.MN)=20240225_BL_HEX-000754_Met_Neg_039; RAW_FILE_NAME(Raw Source Name.MP)=20240223_BL_HEX-000754_Met_Pos_039; RAW_FILE_NAME(Raw Source Name.FN)=20240225_BL_HEX-000754_pFDG_Neg_039
SUBJECT_SAMPLE_FACTORS           	Pool_3	HSA-000037078	condition:NA | treatment:NA | Sample source:microglia	genotype=APP; RAW_FILE_NAME(Raw Source Name.LN)=20240223_BL_HEX-000754_Lipid_Neg_040; RAW_FILE_NAME(Raw Source Name.LP)=20240223_BL_HEX-000754_Lipid_Pos_040; RAW_FILE_NAME(Raw Source Name.MN)=20240225_BL_HEX-000754_Met_Neg_040; RAW_FILE_NAME(Raw Source Name.MP)=20240223_BL_HEX-000754_Met_Pos_040; RAW_FILE_NAME(Raw Source Name.FN)=20240225_BL_HEX-000754_pFDG_Neg_040
#COLLECTION
CO:COLLECTION_SUMMARY            	Microglia isolation was performed using the Magnetic-activated cell sorting
CO:COLLECTION_SUMMARY            	(MACS) system (Miltenyi Biotec). No CD11b enrichment step was performed as the
CO:COLLECTION_SUMMARY            	intrinsic mKate2 reporter is only expressed in TREM2 expressing (microglia)
CO:COLLECTION_SUMMARY            	cells. Hence, fluorescence-activated cell sorting was performed based on
CO:COLLECTION_SUMMARY            	fluorescent TREM2 reporter expression, enabling analysis of low-, mid-, and
CO:COLLECTION_SUMMARY            	high-expressing TREM2 subpopulations from the same mouse.
CO:SAMPLE_TYPE                   	microglia
CO:STORAGE_CONDITIONS            	-80℃
#TREATMENT
TR:TREATMENT_SUMMARY             	Mice were treated with TREM2 agonist antibody (4D9) engineered to cross the
TR:TREATMENT_SUMMARY             	blood brain barrier using an antibody transport vehicle (ATV:4D9). To determine
TR:TREATMENT_SUMMARY             	the effect of this brain-penetrant antibody on microglial subpopulations
TR:TREATMENT_SUMMARY             	stratified by TREM2 expression levels, mice were dosed monthly with 1 mg/kg
TR:TREATMENT_SUMMARY             	ATV:4D9 or isotype control (ATV:Iso) for 4 months. In addition, 30 mg/kg of
TR:TREATMENT_SUMMARY             	fluorodeoxyglucose (FDG) was dosed 1 hour before animal takedown to enable
TR:TREATMENT_SUMMARY             	detection of FDG-monophosphate (FDG-MP) in sorted microglia.
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	Pellets from the sorted subpopulations were reconstituted on ice in 9:1
SP:SAMPLEPREP_SUMMARY            	MeOH:water including internal standards, vortexed for 1 minute, and spun down
SP:SAMPLEPREP_SUMMARY            	for 5 minutes at 10,000 g. Supernatant was transferred to glass vial for
SP:SAMPLEPREP_SUMMARY            	analysis by LC-MS.
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_TYPE           	Reversed phase
CH:INSTRUMENT_NAME               	Agilent 1290 Infinity II
CH:COLUMN_NAME                   	Waters ACQUITY UPLC BEH C18 (100 x 2.1mm,1.7um)
CH:SOLVENT_A                     	60% acetonitrile/40% water; 10 mM ammonium formate; 0.1% formic acid
CH:SOLVENT_B                     	90% isopropyl alcohol/10% acetonitrile; 10 mM ammonium formate; 0.1% formic
CH:SOLVENT_B                     	acid
CH:FLOW_GRADIENT                 	0.0-8.0 min from 45% B to 99% B, 8.0-9.0 min at 99% B, 9.0-9.1 min to 45% B, and
CH:FLOW_GRADIENT                 	9.1-10.0 min at 45% B.
CH:FLOW_RATE                     	0.25 mL/min
CH:COLUMN_TEMPERATURE            	55
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
#MS
MS:INSTRUMENT_NAME               	ABI Sciex 6500+ Qtrap
MS:INSTRUMENT_TYPE               	Triple quadrupole
MS:MS_TYPE                       	ESI
MS:ION_MODE                      	POSITIVE
MS:MS_COMMENTS                   	Curtain gas at 40 psi (positive mode); collision gas was set at medium; ion
MS:MS_COMMENTS                   	spray voltage at 5500 V (positive mode); temperature at 250°C (positive mode);
MS:MS_COMMENTS                   	ion source Gas 1 at 55 psi; ion source Gas 2 at 60 psi; entrance potential at 10
MS:MS_COMMENTS                   	V (positive mode); and  collision cell exit potential at 12.5 V (positive
MS:MS_COMMENTS                   	mode). Quantification was performed using MultiQuant 3.02 (Sciex).
#MS_METABOLITE_DATA
MS_METABOLITE_DATA:UNITS	normalized peak area
MS_METABOLITE_DATA_START
Samples	HSA-000036704	HSA-000036705	HSA-000036706	HSA-000036707	HSA-000036708	HSA-000036709	HSA-000036710	HSA-000036711	HSA-000036712	HSA-000036713	HSA-000036714	HSA-000036715	HSA-000036716	HSA-000036717	HSA-000036718	HSA-000036719	HSA-000036720	HSA-000036721	HSA-000036722	HSA-000036723	HSA-000036724	HSA-000036725	HSA-000036726	HSA-000036727	HSA-000036728	HSA-000036729	HSA-000036730	HSA-000036731	HSA-000036732	HSA-000036733	HSA-000036734	HSA-000036735	HSA-000036736	HSA-000036737	HSA-000036738	HSA-000036739	HSA-000036740	HSA-000037077	HSA-000037078
Factors	condition:low | treatment:ATV Iso | Sample source:microglia	condition:low | treatment:ATV 4D9 | Sample source:microglia	condition:low | treatment:ATV Iso | Sample source:microglia	condition:low | treatment:ATV 4D9 | Sample source:microglia	condition:low | treatment:ATV Iso | Sample source:microglia	condition:low | treatment:ATV 4D9 | Sample source:microglia	condition:low | treatment:ATV 4D9 | Sample source:microglia	condition:low | treatment:ATV 4D9 | Sample source:microglia	condition:low | treatment:ATV Iso | Sample source:microglia	condition:low | treatment:ATV Iso | Sample source:microglia	condition:low | treatment:ATV 4D9 | Sample source:microglia	condition:low | treatment:ATV Iso | Sample source:microglia	condition:mid | treatment:ATV Iso | Sample source:microglia	condition:mid | treatment:ATV 4D9 | Sample source:microglia	condition:mid | treatment:ATV Iso | Sample source:microglia	condition:mid | treatment:ATV 4D9 | Sample source:microglia	condition:mid | treatment:ATV Iso | Sample source:microglia	condition:mid | treatment:ATV 4D9 | Sample source:microglia	condition:mid | treatment:ATV 4D9 | Sample source:microglia	condition:mid | treatment:ATV 4D9 | Sample source:microglia	condition:mid | treatment:ATV Iso | Sample source:microglia	condition:mid | treatment:ATV Iso | Sample source:microglia	condition:mid | treatment:ATV 4D9 | Sample source:microglia	condition:mid | treatment:ATV Iso | Sample source:microglia	condition:high | treatment:ATV Iso | Sample source:microglia	condition:high | treatment:ATV 4D9 | Sample source:microglia	condition:high | treatment:ATV Iso | Sample source:microglia	condition:high | treatment:ATV 4D9 | Sample source:microglia	condition:high | treatment:ATV Iso | Sample source:microglia	condition:high | treatment:ATV 4D9 | Sample source:microglia	condition:high | treatment:ATV 4D9 | Sample source:microglia	condition:high | treatment:ATV 4D9 | Sample source:microglia	condition:high | treatment:ATV Iso | Sample source:microglia	condition:high | treatment:ATV Iso | Sample source:microglia	condition:high | treatment:ATV 4D9 | Sample source:microglia	condition:high | treatment:ATV Iso | Sample source:microglia	condition:NA | treatment:NA | Sample source:microglia	condition:NA | treatment:NA | Sample source:microglia	condition:NA | treatment:NA | Sample source:microglia
1-O-Palmitoyl-Cer(d18:1/18:0)	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
24-Hydroxycholesterol	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
24-Hydroxycholesterol(d7)	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
3-O-SulfoLacCer(d18:1/18:0)	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
4-beta-Hydroxycholesterol	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
7-keto-Cholesterol	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
CE HETE	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
CE HODE	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
CE HpODE	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
CE oxoHETE	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
CE oxoODE	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
CE(16:1)	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
CE(18:1(d7))	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
CE(18:1)	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
CE(18:2)	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
CE(20:4)	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
CE(20:5)	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
CE(22:6)	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Cer(d18:0/16:0)	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Cer(d18:0/18:0)	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Cer(d18:0/24:0)	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Cer(d18:0/24:1)	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Cer(d18:1/16:0(d7))	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Cer(d18:1/16:0)	0.078	0.134	0.124	0.142	0.159	0.088	0.051	0.114	0.111	0.117	0.154	0.126	0.276	0.206	0.349	0.577	0.389	0.497	N.D.	0.298	0.367	0.217	0.455	0.106	0.446	0.245	0.736	1.044	0.683	0.11	0.07	0.811	0.601	0.585	1.15	0.512	0.342	0.42	0.53
Cer(d18:1/18:0)	0.422	1.471	1.452	1.839	1.559	0.18	0.012	1.837	1.446	1.259	1.837	2.06	1.167	0.235	0.925	3.203	1.946	1.576	0.017	0.588	0.508	0.372	0.647	0.034	0.416	0.223	0.525	0.817	0.635	0.035	0.031	0.58	0.478	0.443	0.872	0.376	0.844	1.217	1.213
Cer(d18:1/24:0)	0.065	0.064	0.147	0.057	0.082	0.066	0.055	0.158	0.056	0.086	0.088	0.053	0.452	0.125	0.342	0.407	0.308	0.565	0.063	0.404	0.402	0.271	0.46	0.102	0.399	0.152	0.536	0.639	0.595	0.093	0.083	0.702	0.666	0.56	0.82	0.292	0.386	0.435	0.467
Cer(d18:1/24:1)	0.019	0.076	0.248	0.204	0.158	0.046	0.039	0.212	0.106	0.118	0.159	0.192	0.717	0.115	0.673	0.796	0.547	0.718	N.D.	0.733	0.782	0.388	0.682	0.01	1.191	0.36	1.284	1.717	1.436	0.109	0.032	1.672	1.375	1.247	2.067	0.769	0.659	0.866	0.766
Cholesterol	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Cholesterol(d7)	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Cholesteryl hexoside	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Coenzyme Q10	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
DG(15:0/18:1(d7))	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
DG(16:0_18:1)	0.813	0.535	0.525	0.569	0.45	0.576	0.601	0.586	0.609	0.538	0.755	0.57	0.816	0.652	0.588	0.504	0.776	0.852	0.502	0.598	0.677	0.468	0.471	0.454	0.49	0.493	0.637	0.542	0.668	0.582	0.386	0.662	0.511	0.54	0.672	0.616	0.611	0.613	0.713
DG(16:0_20:4)	N.D.	0.044	0.085	0.102	0.074	0.03	N.D.	0.089	0.065	0.095	0.078	0.085	0.094	0.043	0.068	0.123	0.074	0.081	N.D.	0.168	0.084	0.073	0.096	N.D.	0.129	0.044	0.15	0.242	0.097	N.D.	N.D.	0.245	0.215	0.153	0.297	0.156	0.152	0.137	0.099
DG(18:0_18:1)	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
DG(18:0_20:4)	0.041	0.176	0.277	0.303	0.301	0.081	0.018	0.223	0.343	0.332	0.338	0.548	0.181	0.042	0.126	0.196	0.228	0.192	0.015	0.128	0.199	0.111	0.104	0.011	0.122	0.069	0.099	0.126	0.095	N.D.	0.011	0.255	0.235	0.152	0.205	0.235	0.334	0.293	0.304
DG(18:0_22:6)	0.091	0.717	0.058	0.055	0.055	0.063	0.193	0.536	0.5	0.294	0.119	0.605	0.204	0.229	3.637	0.074	0.176	0.7	0.114	0.606	0.168	0.184	0.268	0.118	0.656	0.189	2.153	0.207	0.426	0.318	0.148	0.59	0.564	0.771	0.114	0.111	0.256	0.424	0.414
DG(18:1/18:1)	1.539	2.006	1.88	1.643	1.409	1.907	1.595	1.652	1.388	1.928	1.28	1.68	1.844	1.462	1.686	1.551	2.02	2.263	1.635	2.273	1.741	1.398	1.402	1.574	1.561	1.32	2.112	1.476	1.56	1.688	1.597	1.384	1.482	1.721	2.045	1.579	2.188	2.174	1.583
DG(18:1_20:4)	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
GB3(d18:1/16:0)	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
GB3(d18:1/18:0(d3))	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
GB3(d18:1/18:0)	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
GB3(d18:1/24:0)	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
GB3(d18:1/24:1)	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
GlcCer(d18:1(d5)/18:0)	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
GlcCer(d18:1/16:0(d3))	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Glucosylsphingosine(d5)	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
HexCer(d18:1/12:0)	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
HexCer(d18:1/16:0)	N.D.	N.D.	N.D.	N.D.	0.017	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	0.012	0.015	0.023	0.018	0.017	0.006	N.D.	0.011	N.D.	0.023	N.D.	0.034	N.D.	0.046	0.067	0.051	N.D.	N.D.	0.058	0.022	0.019	0.045	0.021	0.021	0.015	N.D.
HexCer(d18:1/18:0)	0.013	0.057	0.116	0.14	0.22	0.018	N.D.	0.084	0.071	0.078	0.12	0.098	0.072	0.02	0.05	0.105	0.101	0.067	N.D.	0.042	0.03	0.035	0.049	N.D.	0.043	0.011	0.068	0.062	0.073	0.013	N.D.	0.045	0.031	0.034	0.063	0.029	0.086	0.119	0.098
HexCer(d18:1/22:0)	0.026	0.054	0.186	0.222	0.259	0.028	N.D.	0.092	0.076	0.075	0.23	0.146	0.136	0.023	0.132	0.153	0.16	0.126	N.D.	0.152	0.07	0.037	0.121	N.D.	0.134	0.03	0.167	0.16	0.186	0.008	N.D.	0.145	0.122	0.146	0.173	0.115	0.216	0.169	0.205
HexCer(d18:1/24:0)	0.026	0.052	0.238	0.166	0.294	0.033	0.004	0.112	0.122	0.092	0.201	0.161	0.103	N.D.	0.082	0.126	0.125	0.13	N.D.	0.078	0.054	0.031	0.067	0.003	0.094	0.009	0.152	0.1	0.12	0.006	0.003	0.152	0.077	0.085	0.145	0.058	0.182	0.168	0.141
HexCer(d18:1/24:1)	0.194	0.379	1.237	1.409	2.016	0.149	0.007	0.519	0.578	0.53	1.323	0.981	0.375	0.026	0.379	0.405	0.556	0.456	N.D.	0.202	0.132	0.068	0.198	0.01	0.165	0.041	0.222	0.291	0.388	0.038	0.005	0.249	0.141	0.093	0.216	0.144	0.61	0.674	0.592
Hexosylsphingosine	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
LPC(16:0)	0.021	0.037	0.039	0.035	0.042	0.024	0.024	0.035	0.036	0.04	0.049	0.044	0.05	0.025	0.04	0.041	0.056	0.036	0.015	0.033	0.028	0.024	0.034	0.013	0.052	0.019	0.053	0.046	0.038	0.016	0.023	0.036	0.046	0.037	0.041	0.034	0.048	0.041	0.037
LPC(16:1)	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
LPC(18:0)	0.191	0.186	0.112	0.112	0.102	0.178	0.339	0.183	0.126	0.207	0.168	0.183	0.124	0.173	0.09	0.137	0.126	0.132	0.187	0.137	0.13	0.184	0.118	0.162	0.117	0.101	0.126	0.102	0.07	0.176	0.197	0.146	0.152	0.139	0.128	0.18	0.167	0.168	0.156
LPC(18:1(d7))	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
LPC(18:1)	0.021	0.03	0.029	0.031	0.036	0.021	0.037	0.032	0.03	0.031	0.033	0.031	0.031	0.024	0.025	0.029	0.033	0.03	0.016	0.026	0.023	0.022	0.025	0.017	0.027	0.019	0.029	0.03	0.026	0.017	0.021	0.024	0.024	0.025	0.026	0.027	0.036	0.028	0.028
LPC(20:4)	0.002	0.007	0.006	0.005	0.004	0.003	0.002	0.005	0.004	0.004	0.006	0.006	0.016	0.007	0.01	0.015	0.014	0.014	N.D.	0.011	0.008	0.008	0.012	N.D.	0.011	0.006	0.015	0.02	0.014	0.002	0.001	0.013	0.009	0.009	0.011	0.01	0.014	0.012	0.011
LPC(22:6)	0.001	0.002	0.003	0.002	0.002	N.D.	0.001	0.003	0.002	0.002	0.004	0.001	0.003	0.002	0.004	0.003	0.004	0.003	N.D.	0.002	0.003	0.001	0.003	N.D.	0.003	0.003	0.003	0.004	0.002	0.001	N.D.	0.003	0.003	0.003	0.002	0.002	0.001	0.003	0.002
LPC(24:0)	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
LPC(24:1)	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
LPC(26:0)	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
LPC(26:1)	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
LacCer(d18:1/16:0)	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
LacCer(d18:1/17:0)	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
LacCer(d18:1/18:0)	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
LacCer(d18:1/24:0)	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
LacCer(d18:1/24:1)	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Lactosylsphingosine	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
MG(16:0)	23.158	24.406	17.888	20.358	21.148	15.693	17.018	18.591	22.608	22.908	25.223	17.874	28.855	12.221	22.119	22.493	29.31	21.002	14.693	16.745	25.027	18.248	13.538	10.866	22.028	14.65	16.946	27.101	20.929	20.685	11.661	21.882	17.898	15.809	16.416	13.301	25.365	25.828	27.845
MG(16:1)	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
MG(18:0)	117.845	117.038	91.158	101.767	95.795	78.134	81.206	110.485	102.877	113.769	113.343	94.042	133.515	75.31	97.121	106.514	136.268	111.635	80.293	108.478	128.378	92.866	76.687	70.745	98.907	83.167	85.909	119.796	109.966	109.313	66.64	121.898	85.446	78.249	90.563	88.518	105.267	118.321	117.272
MG(18:1(d7))	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
MG(18:1)	1.996	1.831	1.792	2.025	1.91	1.643	1.318	1.839	1.677	1.998	2.261	1.685	2.411	1.417	1.648	2.048	2.71	2.083	1.289	1.793	2.046	1.967	1.182	1.073	1.904	1.472	1.683	2.258	1.914	2.082	1.16	2.188	1.689	1.339	1.469	1.473	2.165	2.317	2.489
MG(20:4)	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
N-Oleoylethanolamine	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
N-Palmitoyl-O-phosphocholineserine	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
PC(15:0/18:1(d7))	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
PC(16:0/5:0(CHO))	0.004	0.003	0.01	0.005	0.005	0.007	0.004	0.009	0.007	0.004	0.004	0.004	0.026	0.007	0.022	0.035	0.02	0.024	0.005	0.009	0.009	0.008	0.016	0.003	0.019	0.006	0.029	0.021	0.03	0.005	0.005	0.017	0.014	0.016	0.017	0.032	0.025	0.017	0.018
PC(16:0/9:0(CHO))	0.021	0.041	0.071	0.037	0.047	0.038	0.025	0.07	0.05	0.032	0.032	0.034	0.101	0.023	0.066	0.111	0.07	0.079	0.027	0.033	0.015	0.016	0.042	0.018	0.054	0.019	0.072	0.043	0.063	0.014	0.034	0.03	0.053	0.047	0.035	0.07	0.07	0.043	0.037
PC(16:0/9:0(COOH))	0.006	0.012	0.013	0.007	0.008	0.01	0.01	0.021	0.012	0.009	0.009	0.012	0.019	0.006	0.014	0.019	0.025	0.016	0.007	0.007	0.008	0.008	0.009	0.009	0.013	0.008	0.014	0.009	0.013	0.004	0.009	0.007	0.012	0.011	0.01	0.013	0.012	0.013	0.01
PC(18:0/20:4(OH[S]))	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
PC(18:0/20:4(OOH[S]))	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
PC(34:1)	0.37	1.358	2.186	2.137	2.303	0.375	0.04	1.215	1.632	1.608	2.21	2.068	1.868	0.719	1.823	3.515	2.321	2.35	0.025	1.345	1.25	0.807	1.524	0.025	1.777	0.61	2.364	2.739	2.173	0.172	0.039	2.34	1.99	1.912	2.63	1.34	2.201	1.945	1.994
PC(36:1)	0.126	0.365	0.662	0.658	0.821	0.112	0.014	0.376	0.448	0.405	0.736	0.594	0.346	0.082	0.302	0.512	0.424	0.411	0.011	0.201	0.151	0.105	0.207	0.012	0.206	0.049	0.235	0.238	0.232	0.031	0.011	0.215	0.18	0.17	0.231	0.135	0.4	0.363	0.362
PC(36:2)	0.067	0.171	0.265	0.236	0.237	0.083	0.063	0.152	0.182	0.184	0.254	0.238	0.299	0.125	0.288	0.479	0.316	0.329	0.038	0.206	0.185	0.134	0.219	0.031	0.306	0.119	0.375	0.45	0.35	0.054	0.05	0.397	0.344	0.327	0.464	0.226	0.401	0.296	0.308
PC(36:4)	0.054	0.235	0.37	0.335	0.298	0.065	0.009	0.213	0.265	0.249	0.342	0.348	1.235	0.775	1.371	2.007	1.382	1.578	0.006	1.309	1.101	0.822	1.457	0.006	1.975	0.707	2.415	3.009	2.359	0.191	0.01	2.606	2.093	2.096	3.13	1.452	1.664	1.549	1.581
PC(38:4)	0.038	0.17	0.285	0.244	0.214	0.04	0.009	0.146	0.197	0.179	0.253	0.232	0.388	0.191	0.415	0.709	0.441	0.528	0.005	0.38	0.291	0.209	0.376	0.006	0.417	0.134	0.462	0.5	0.419	0.028	0.009	0.494	0.37	0.383	0.531	0.268	0.429	0.379	0.398
PC(38:6)	0.032	0.104	0.185	0.191	0.199	0.031	0.001	0.089	0.131	0.113	0.169	0.181	0.199	0.071	0.189	0.422	0.251	0.263	0.001	0.116	0.107	0.075	0.123	0.001	0.15	0.052	0.183	0.21	0.181	0.015	0.003	0.196	0.154	0.16	0.232	0.107	0.186	0.176	0.176
PC(40:5)	0.002	0.007	0.012	0.009	0.009	0.004	0.002	0.005	0.01	0.009	0.008	0.01	0.013	0.006	0.015	0.027	0.014	0.016	0.001	0.009	0.009	0.007	0.01	0.001	0.011	0.005	0.015	0.015	0.013	0.002	0.001	0.018	0.012	0.011	0.016	0.01	0.014	0.011	0.01
PC(40:6)	0.016	0.052	0.091	0.09	0.107	0.016	0.001	0.048	0.062	0.052	0.083	0.085	0.082	0.022	0.077	0.169	0.1	0.106	0.001	0.045	0.037	0.028	0.05	0	0.044	0.014	0.047	0.051	0.045	0.004	0.002	0.048	0.037	0.035	0.052	0.028	0.071	0.063	0.061
PC(O-16:0/0:0)	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
PC(O-16:0/2:0)	N.D.	0.004	0.004	0.003	0.003	0.002	N.D.	0.004	0.003	0.001	0.003	0.005	0.006	0.002	0.005	0.007	0.008	0.004	N.D.	0.002	0.002	0.001	0.004	N.D.	0.003	0.002	0.008	0.009	0.006	0.001	0.001	0.006	0.006	0.006	0.007	0.006	0.007	0.003	0.005
PC(O-18:0/2:0)	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
PE(15:0/18:1(d7))	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
PE(18:0/20:4(OH[S]))	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
PE(18:0/20:4(OOH[S]))	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
PE(34:1)	0.229	0.949	1.504	1.641	1.764	0.272	N.D.	1.154	1.66	1.251	1.462	1.537	0.68	0.247	0.877	1.266	1.149	1.061	N.D.	0.589	0.653	0.355	0.669	0.008	1.019	0.28	1.463	1.474	1.31	0.094	0.014	1.559	1.131	1.124	1.575	0.721	1.252	1.308	0.916
PE(36:1)	0.38	1.171	2.349	2.888	3.325	0.299	0.005	1.384	1.742	1.18	2.165	1.983	0.983	0.153	0.836	1.205	1.286	1.334	N.D.	0.591	0.503	0.358	0.744	0.013	0.653	0.116	0.875	0.912	0.95	0.089	N.D.	0.829	0.726	0.63	0.924	0.404	1.367	1.056	0.865
PE(36:2)	0.341	1.158	1.949	2.337	2.227	0.448	0.027	1.273	2.283	1.949	1.953	2.133	0.755	0.175	0.801	1.137	1.149	0.949	0.023	0.549	0.589	0.324	0.541	0.021	0.656	0.196	0.887	1.009	1.049	0.052	0.031	0.967	0.759	0.693	1.071	0.53	1.391	1.257	1.038
PE(36:4)	0.111	0.508	0.704	0.639	0.561	0.119	N.D.	0.414	0.624	0.544	0.592	0.719	0.805	0.46	0.99	1.339	1.062	1.197	N.D.	0.786	0.975	0.68	1.1	N.D.	1.585	0.596	2.059	2.817	2.168	0.137	0.009	2.689	1.976	1.919	2.589	1.539	1.433	1.389	1.193
PE(38:4)	0.583	2.18	4.221	4.249	4.046	0.562	0.009	2.242	2.939	2.373	3.101	3.762	3.058	1.014	3.565	6.091	4.028	4.788	0.005	2.661	2.445	1.637	2.764	0.024	3.639	0.896	4.395	4.568	3.932	0.217	0.011	4.412	3.239	3.253	4.511	2.725	3.888	3.458	3.075
PE(38:5)	0.117	0.747	0.94	1.045	0.761	0.156	0.011	0.609	0.974	0.81	0.814	0.991	0.525	0.201	0.628	0.99	0.856	0.803	N.D.	0.449	0.554	0.357	0.501	N.D.	0.754	0.281	0.862	1.329	1.131	0.058	0.007	1.216	0.999	0.878	1.329	0.567	0.947	0.913	0.809
PE(38:6)	0.313	0.927	1.64	2.043	2.205	0.267	0.007	0.761	1.388	1.08	1.263	1.879	1.137	0.413	1.226	2.434	1.59	1.669	N.D.	0.798	0.673	0.657	0.891	0.008	1.457	0.46	1.723	2.208	1.763	0.119	0.007	1.981	1.492	1.52	1.992	0.88	1.561	1.439	1.272
PE(40:4)	0.115	0.54	0.887	0.885	1.118	0.168	N.D.	0.458	0.644	0.447	0.69	0.745	0.408	0.103	0.489	0.791	0.591	0.698	N.D.	0.412	0.313	0.272	0.471	0.009	0.381	0.126	0.643	0.559	0.442	0.028	N.D.	0.711	0.421	0.361	0.497	0.277	0.509	0.59	0.39
PE(40:5)	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
PE(40:6)	1.096	3.339	6.507	7.504	7.848	1.003	0.02	2.88	5.127	3.495	4.715	5.886	3.58	0.709	4.051	8.082	4.964	5.534	0.008	2.177	2.101	1.429	2.515	0.018	2.679	0.616	3.237	2.827	2.723	0.214	0.024	2.92	2.108	1.968	2.74	1.676	3.819	3.581	2.99
PE(40:7)	0.102	0.274	0.497	0.583	0.621	0.063	N.D.	0.256	0.397	0.345	0.311	0.483	0.471	0.124	0.59	1.138	0.75	0.748	N.D.	0.24	0.26	0.166	0.354	N.D.	0.438	0.103	0.586	0.632	0.639	0.023	N.D.	0.695	0.599	0.457	0.791	0.308	0.541	0.561	0.413
Palmitoylethanolamine	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
SM(d18:1(d9)/18:1)	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
SM(d18:1/16:0)	0.15	0.21	0.206	0.151	0.136	0.174	0.258	0.195	0.186	0.21	0.189	0.205	0.29	0.197	0.242	0.28	0.229	0.251	0.174	0.276	0.229	0.218	0.254	0.158	0.336	0.151	0.462	0.394	0.332	0.152	0.175	0.424	0.437	0.379	0.428	0.304	0.358	0.312	0.29
SM(d18:1/18:0)	0.095	0.3	0.618	0.552	0.58	0.082	0.026	0.282	0.419	0.349	0.523	0.482	0.268	0.081	0.239	0.457	0.305	0.327	0.015	0.176	0.139	0.08	0.15	0.014	0.171	0.058	0.216	0.194	0.211	0.03	0.017	0.166	0.165	0.14	0.19	0.11	0.331	0.311	0.273
SM(d18:1/24:0)	0.008	0.041	0.093	0.024	0.023	0.022	0.017	0.031	0.04	0.022	0.026	0.022	0.153	0.012	0.193	0.067	0.051	0.085	0.015	0.056	0.049	0.027	0.049	0.005	0.091	0.025	0.19	0.086	0.073	0.007	0.029	0.075	0.118	0.092	0.088	0.057	0.262	0.061	0.047
SM(d18:1/24:1)	0.021	0.076	0.166	0.127	0.133	0.032	0.01	0.074	0.084	0.078	0.133	0.107	0.24	0.064	0.219	0.209	0.191	0.21	0.006	0.203	0.173	0.093	0.177	0.007	0.313	0.077	0.394	0.355	0.31	0.02	0.025	0.351	0.301	0.279	0.352	0.2	0.289	0.218	0.184
Sitosteryl hexoside	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Sphinganine	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Sphinganine 1-phosphate	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Sphingosine	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Sphingosine 1-phosphate	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Sphingosine 1-phosphate-d7	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Sphingosine 1-phosphocholine	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
Sphingosine(d17:1)	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
TG(15:0/18:1(d7)/15:0)	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
TG(18:0_36:2)	1.151	1.209	0.943	0.838	0.817	1.01	0.719	1.056	0.9	0.966	0.79	1.082	0.883	1.294	0.811	0.911	0.882	0.933	0.713	1.178	0.894	1.004	0.839	0.823	0.84	0.948	0.888	0.821	0.778	0.931	0.775	1.087	0.945	1.083	0.795	1.203	1.131	1.206	1.194
TG(18:1_34:2)	3.667	3.878	3.115	2.605	2.645	3.075	2.185	3.52	2.74	3.256	2.781	3.15	3.004	4.024	2.667	2.863	2.935	2.972	2.201	3.781	2.757	2.655	2.993	2.392	2.408	2.792	2.828	2.754	2.696	2.731	2.52	3.244	2.904	3.302	2.803	3.865	3.523	3.553	3.886
TG(18:1_34:3)	0.52	0.595	0.465	0.393	0.374	0.492	0.356	0.52	0.444	0.534	0.401	0.51	0.44	0.592	0.414	0.432	0.419	0.496	0.331	0.502	0.424	0.489	0.459	0.362	0.389	0.435	0.428	0.445	0.347	0.419	0.321	0.479	0.398	0.52	0.434	0.579	0.535	0.629	0.633
TG(20:4_32:1)	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
TG(20:4_34:2)	0.051	0.064	0.044	0.048	0.044	0.047	0.029	0.06	0.035	0.037	0.049	0.055	0.046	0.067	0.038	0.038	0.052	0.048	0.034	0.048	0.048	0.046	0.058	0.034	0.035	0.052	0.05	0.043	0.04	0.035	0.039	0.037	0.04	0.062	0.037	0.046	0.056	0.046	0.043
TG(20:4_34:3)	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
TG(20:4_36:0)	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
TG(20:4_36:2)	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
TG(20:4_36:3)	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
TG(22:6_36:2)	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
TG(22:6_38:1)	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
TG(22:6_38:2)	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
lyso-GB3	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
lyso-GB3-d7	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
lyso-GB4	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
MS_METABOLITE_DATA_END
#METABOLITES
METABOLITES_START
metabolite_name	Expected RT	MS1_mz	MS2_mz
1-O-Palmitoyl-Cer(d18:1/18:0)	8.1	786.8	502.5
24-Hydroxycholesterol	2	385.3	367.3
24-Hydroxycholesterol(d7)	2	392.3	367.3
3-O-SulfoLacCer(d18:1/18:0)	5.2	970.8	548.5
4-beta-Hydroxycholesterol	2.14	420.3	385.3
7-keto-Cholesterol	3.2	401.3	383.3
CE HETE	7.27	706.6	369.2
CE HODE	7.32	682.6	369.2
CE HpODE	6.76	698.6	369.2
CE oxoHETE	6.65	704.6	369.2
CE oxoODE	6.73	680.6	369.2
CE(16:1)	8.42	640.6	369.3
CE(18:1(d7))	8.64	675.2	369.4
CE(18:1)	8.64	668.6	369.3
CE(18:2)	8.43	666.6	369.3
CE(20:4)	8.3	690.6	369.3
CE(20:5)	8.09	688.6	369.3
CE(22:6)	8.16	714.6	369.3
Cer(d18:0/16:0)	6.29	540.6	284.3
Cer(d18:0/18:0)	6.79	568.7	284.3
Cer(d18:0/24:0)	7.81	652.9	284.3
Cer(d18:0/24:1)	7.07	650.9	284.4
Cer(d18:1/16:0(d7))	5.71	545.5	271.4
Cer(d18:1/16:0)	5.89	538.5	264.3
Cer(d18:1/18:0)	6.39	566.6	264.3
Cer(d18:1/24:0)	7.41	650.6	264.3
Cer(d18:1/24:1)	7.07	648.6	264.3
Cholesterol	5.13	369.3	369.3
Cholesterol(d7)	5.13	376.2	376.2
Cholesteryl hexoside	3.91	566.6	369.3
Coenzyme Q10	7.75	863.3	197.2
DG(15:0/18:1(d7))	6.08	605.6	346.5
DG(16:0_18:1)	6.5	612.4	313.3
DG(16:0_20:4)	6.1	634.5	313.3
DG(18:0_18:1)	7.02	640.4	341.3
DG(18:0_20:4)	6.6	662.5	341.3
DG(18:0_22:6)	6.1	686.6	341.3
DG(18:1_20:4)	6.12	660.5	339.3
DG(18:1/18:1)	6.66	638.4	339.3
GB3(d18:1/16:0)	5.2	1025	520.5
GB3(d18:1/18:0(d3))	5.51	1056	551.6
GB3(d18:1/18:0)	5.51	1053	548.6
GB3(d18:1/24:0)	6.95	1137	632.6
GB3(d18:1/24:1)	6.55	1135	630.6
GlcCer(d18:1(d5)/18:0)	6	733.6	269.3
GlcCer(d18:1/16:0(d3))	5.5	703.7	264.3
Glucosylsphingosine(d5)	1.3	467.2	269.3
HexCer(d18:1/12:0)	4.26	644.5	264.3
HexCer(d18:1/16:0)	5.5	700.6	264.3
HexCer(d18:1/18:0)	6	728.6	264.3
HexCer(d18:1/22:0)	6.7	784.7	264.4
HexCer(d18:1/24:0)	7.14	812.7	264.3
HexCer(d18:1/24:1)	6.78	810.7	264.3
Hexosylsphingosine	1.3	462.3	264.2
LacCer(d18:1/16:0)	5.31	862.6	264.3
LacCer(d18:1/17:0)	5.51	876.6	264.3
LacCer(d18:1/18:0)	5.84	890.7	264.3
LacCer(d18:1/24:0)	7	974.8	264.3
LacCer(d18:1/24:1)	6.64	972.7	264.3
Lactosylsphingosine	1.4	624.4	264.3
LPC(16:0)	1.85	496.3	184.1
LPC(16:1)	1.48	494.5	184.1
LPC(18:0)	2.47	524.3	184.1
LPC(18:1(d7))	1.91	529.3	184.1
LPC(18:1)	1.9	522.3	184.1
LPC(20:4)	1.45	544.3	184.1
LPC(22:6)	1.35	568.3	184.1
LPC(24:0)	4.53	608.5	184.1
LPC(24:1)	3.8	606.5	184.1
LPC(26:0)	5.2	636.5	104.1
LPC(26:1)	4.7	634.5	104.1
lyso-GB3	1.21	786.6	264.3
lyso-GB3-d7	1.21	793.5	271.3
lyso-GB4	1.04	990.6	264.3
MG(16:0)	2.48	348.3	239.3
MG(16:1)	2	346.3	237.3
MG(18:0)	3.32	376.3	267.3
MG(18:1(d7))	2.5	381.3	272.5
MG(18:1)	2.56	374.3	265.3
MG(20:4)	1.96	396.3	287.3
N-Oleoylethanolamine	2.2	326.3	62.1
N-Palmitoyl-O-phosphocholineserine		509.5	184.1
Palmitoylethanolamine	2.2	300.3	62.1
PC(15:0/18:1(d7))	5.37	754.6	184.1
PC(16:0/5:0(CHO))	2	594.5	184.1
PC(16:0/9:0(CHO))	2	650.4	184.1
PC(16:0/9:0(COOH))	2.1	666.4	184.1
PC(18:0/20:4(OH[S]))	4.12	826.6	184.1
PC(18:0/20:4(OOH[S]))	4.11	842.6	184.1
PC(34:1)	5.7	760.6	184.1
PC(36:1)	6.1	788.6	184.1
PC(36:2)	5.69	786.6	184.1
PC(36:4)	5.2	782.6	184.1
PC(38:4)	5.48	810.6	184.1
PC(38:6)	4.92	806.6	184.1
PC(40:5)	5.9	836.6	184.1
PC(40:6)	5.46	834.6	184.1
PC(O-16:0/0:0)	1.6	482.3	184.1
PC(O-16:0/2:0)	1.8	524.3	184.2
PC(O-18:0/2:0)	2.37	552.5	184.1
PE(15:0/18:1(d7))	5.52	711.6	570.5
PE(18:0/20:4(OH[S]))	4.28	784.5	643.4
PE(18:0/20:4(OOH[S]))	4.23	800.5	659.4
PE(34:1)	5.8	718.6	577.6
PE(36:1)	6.22	746.6	605.5
PE(36:2)	5.8	744.6	603.5
PE(36:4)	5.2	740.6	599.5
PE(38:4)	5.77	768.6	627.5
PE(38:5)	5.2	766.6	625.5
PE(38:6)	5.07	764.6	623.5
PE(40:4)	6	796.6	655.5
PE(40:5)	5.8	794.6	635.5
PE(40:6)	5.59	792.6	651.5
PE(40:7)	5	790.6	649.5
Sitosteryl hexoside	4.36	594.6	397.4
SM(d18:1(d9)/18:1)	5.33	738.7	184.1
SM(d18:1/16:0)	5.33	703.6	184.1
SM(d18:1/18:0)	5.89	731.6	184.1
SM(d18:1/24:0)	7.07	815.7	184.1
SM(d18:1/24:1)	6.5	813.7	184.1
Sphinganine	1.88	302.2	284.3
Sphinganine 1-phosphate	1.68	382.3	284.3
Sphingosine	1.68	300.2	264.3
Sphingosine 1-phosphate	1.6	380.3	264.3
Sphingosine 1-phosphate-d7	1.6	387.3	271.3
Sphingosine 1-phosphocholine	1.63	465.5	184.1
Sphingosine(d17:1)	1.33	286.2	250.3
TG(15:0/18:1(d7)/15:0)	8.26	829.8	570.8
TG(18:0_36:2)	8.63	904.7	603.4
TG(18:1_34:2)	8.27	874.7	575.4
TG(18:1_34:3)	8.09	872.7	573.4
TG(20:4_32:1)	7.99	870.6	549.3
TG(20:4_34:2)	7.99	896.6	575.3
TG(20:4_34:3)	7.63	894.6	573.3
TG(20:4_36:0)	8.42	928.8	607.5
TG(20:4_36:2)	8.22	924.7	603.4
TG(20:4_36:3)	7.98	922.7	601.4
TG(22:6_36:2)	8	948.7	603.4
TG(22:6_38:1)	8.29	978.7	633.4
TG(22:6_38:2)	8.1	976.7	631.4
METABOLITES_END
#END