#METABOLOMICS WORKBENCH plyush1993_20250929_165907 DATATRACK_ID:6505 STUDY_ID:ST004265 ANALYSIS_ID:AN007098 PROJECT_ID:PR002692
VERSION             	1
CREATED_ON             	October 8, 2025, 11:29 am
#PROJECT
PR:PROJECT_TITLE                 	Seasonal Signatures in Asparagopsis taxiformis (Rhodophyta) Holobiont: Linking
PR:PROJECT_TITLE                 	Epiphytic Microbial Communities, Metabolite Profiles, and Bioactivity
PR:PROJECT_SUMMARY               	The epiphytic microbiome of seaweed plays a central role in host physiology,
PR:PROJECT_SUMMARY               	defense, and adaptation. This host–microbe association, known as the
PR:PROJECT_SUMMARY               	holobiont, is shaped by environmental factors such as temperature and
PR:PROJECT_SUMMARY               	seasonality, which also influence bioactive compound production in both marine
PR:PROJECT_SUMMARY               	and terrestrial organisms. Asparagopsis taxiformis is a red macroalga with
PR:PROJECT_SUMMARY               	cosmopolitan distribution, high invasive potential, and growing biotechnological
PR:PROJECT_SUMMARY               	interest. We conducted a two-year, bi-monthly survey at two Israeli
PR:PROJECT_SUMMARY               	Mediterranean sites to examine seasonal links between its epiphytic microbiome
PR:PROJECT_SUMMARY               	and metabolome. Seawater temperature emerged as the primary driver of variation
PR:PROJECT_SUMMARY               	in both microbial composition and metabolite profiles, with host lineage and
PR:PROJECT_SUMMARY               	site showing secondary effects. Warmer periods correlated with higher bacterial
PR:PROJECT_SUMMARY               	diversity, elevated brominated metabolites, and stronger antibacterial activity.
PR:PROJECT_SUMMARY               	These patterns suggest that A. taxiformis adjusts its chemical output in
PR:PROJECT_SUMMARY               	response to seasonal microbial dynamics and provide a framework for optimizing
PR:PROJECT_SUMMARY               	bioactive compound production under controlled cultivation.
PR:INSTITUTE                     	Haifa University
PR:DEPARTMENT                    	Marine Biology
PR:LAST_NAME                     	Omri
PR:FIRST_NAME                    	Nahor
PR:ADDRESS                       	Multipurpose Building, University of Haifa, 199 Aba Khoushy Ave., Mount Carmel,
PR:ADDRESS                       	Haifa, Israel
PR:EMAIL                         	nahoro27@gmail.com
PR:PHONE                         	+972546442703
PR:CONTRIBUTORS                  	Tal Luzzatto Knaan, Ivan Plyushchenko
#STUDY
ST:STUDY_TITLE                   	Seasonal Signatures in Asparagopsis taxiformis (Rhodophyta) Holobiont: Linking
ST:STUDY_TITLE                   	Epiphytic Microbial Communities, Metabolite Profiles, and Bioactivity
ST:STUDY_SUMMARY                 	The epiphytic microbiome of seaweed plays a central role in host physiology,
ST:STUDY_SUMMARY                 	defense, and adaptation. This host–microbe association, known as the
ST:STUDY_SUMMARY                 	holobiont, is shaped by environmental factors such as temperature and
ST:STUDY_SUMMARY                 	seasonality, which also influence bioactive compound production in both marine
ST:STUDY_SUMMARY                 	and terrestrial organisms. Asparagopsis taxiformis is a red macroalga with
ST:STUDY_SUMMARY                 	cosmopolitan distribution, high invasive potential, and growing biotechnological
ST:STUDY_SUMMARY                 	interest. We conducted a two-year, bi-monthly survey at two Israeli
ST:STUDY_SUMMARY                 	Mediterranean sites to examine seasonal links between its epiphytic microbiome
ST:STUDY_SUMMARY                 	and metabolome. Seawater temperature emerged as the primary driver of variation
ST:STUDY_SUMMARY                 	in both microbial composition and metabolite profiles, with host lineage and
ST:STUDY_SUMMARY                 	site showing secondary effects. Warmer periods correlated with higher bacterial
ST:STUDY_SUMMARY                 	diversity, elevated brominated metabolites, and stronger antibacterial activity.
ST:STUDY_SUMMARY                 	These patterns suggest that A. taxiformis adjusts its chemical output in
ST:STUDY_SUMMARY                 	response to seasonal microbial dynamics and provide a framework for optimizing
ST:STUDY_SUMMARY                 	bioactive compound production under controlled cultivation.
ST:INSTITUTE                     	Haifa University
ST:DEPARTMENT                    	Marine Biology
ST:LAST_NAME                     	Omri
ST:FIRST_NAME                    	Nahor
ST:ADDRESS                       	Multipurpose Building, University of Haifa, 199 Aba Khoushy Ave., Mount Carmel,
ST:ADDRESS                       	Haifa, Israel
ST:EMAIL                         	nahoro27@gmail.com
ST:PHONE                         	+972546442703
#SUBJECT
SU:SUBJECT_TYPE                  	Plant
SU:SUBJECT_SPECIES               	Asparagopsis taxiformis
SU:TAXONOMY_ID                   	260499
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data
SUBJECT_SAMPLE_FACTORS           	-	Blank273	Injection order:1 | Sample source:blank | Site:no | Month:no | season:no | year:no | Temp:no | Fractiontype:no	RAW_FILE_NAME(filename)=Blank 1 neg 5 mcL _Y-F9_1_3273.mzXML
SUBJECT_SAMPLE_FACTORS           	-	Blank339	Injection order:65 | Sample source:blank | Site:no | Month:no | season:no | year:no | Temp:no | Fractiontype:no	RAW_FILE_NAME(filename)=Blank 10 neg 5 mcL_Y-F9_1_3339.mzXML
SUBJECT_SAMPLE_FACTORS           	-	Blank461	Injection order:164 | Sample source:blank | Site:no | Month:no | season:no | year:no | Temp:no | Fractiontype:no	RAW_FILE_NAME(filename)=Blank 10 neg 5 mcL_Y-F9_1_3461.mzXML
SUBJECT_SAMPLE_FACTORS           	-	Blank394	Injection order:99 | Sample source:blank | Site:no | Month:no | season:no | year:no | Temp:no | Fractiontype:no	RAW_FILE_NAME(filename)=Blank 11 neg 5 mcL _Y-F9_1_3394.mzXML
SUBJECT_SAMPLE_FACTORS           	-	Blank346	Injection order:72 | Sample source:blank | Site:no | Month:no | season:no | year:no | Temp:no | Fractiontype:no	RAW_FILE_NAME(filename)=Blank 11 neg 5 mcL_Y-F9_1_3346.mzXML
SUBJECT_SAMPLE_FACTORS           	-	Blank353	Injection order:79 | Sample source:blank | Site:no | Month:no | season:no | year:no | Temp:no | Fractiontype:no	RAW_FILE_NAME(filename)=Blank 12 neg 5 mcL_Y-F9_1_3353.mzXML
SUBJECT_SAMPLE_FACTORS           	-	Blank401	Injection order:106 | Sample source:blank | Site:no | Month:no | season:no | year:no | Temp:no | Fractiontype:no	RAW_FILE_NAME(filename)=Blank 12 neg 5 mcL_Y-F9_1_3401.mzXML
SUBJECT_SAMPLE_FACTORS           	-	Blank361	Injection order:87 | Sample source:blank | Site:no | Month:no | season:no | year:no | Temp:no | Fractiontype:no	RAW_FILE_NAME(filename)=Blank 13 neg 5 mcL_Y-F9_1_3361.mzXML
SUBJECT_SAMPLE_FACTORS           	-	Blank408	Injection order:113 | Sample source:blank | Site:no | Month:no | season:no | year:no | Temp:no | Fractiontype:no	RAW_FILE_NAME(filename)=Blank 13 neg 5 mcL_Y-F9_1_3408.mzXML
SUBJECT_SAMPLE_FACTORS           	-	Blank369	Injection order:94 | Sample source:blank | Site:no | Month:no | season:no | year:no | Temp:no | Fractiontype:no	RAW_FILE_NAME(filename)=Blank 14 neg 5 mcL_Y-F9_1_3369.mzXML
SUBJECT_SAMPLE_FACTORS           	-	Blank416	Injection order:121 | Sample source:blank | Site:no | Month:no | season:no | year:no | Temp:no | Fractiontype:no	RAW_FILE_NAME(filename)=Blank 14 neg 5 mcL_Y-F9_1_3416.mzXML
SUBJECT_SAMPLE_FACTORS           	-	Blank374	Injection order:98 | Sample source:blank | Site:no | Month:no | season:no | year:no | Temp:no | Fractiontype:no	RAW_FILE_NAME(filename)=Blank 15 neg 5 mcL_Y-F9_1_3374.mzXML
SUBJECT_SAMPLE_FACTORS           	-	Blank423	Injection order:128 | Sample source:blank | Site:no | Month:no | season:no | year:no | Temp:no | Fractiontype:no	RAW_FILE_NAME(filename)=Blank 15 neg 5 mcL_Y-F9_1_3423.mzXML
SUBJECT_SAMPLE_FACTORS           	-	Blank430	Injection order:135 | Sample source:blank | Site:no | Month:no | season:no | year:no | Temp:no | Fractiontype:no	RAW_FILE_NAME(filename)=Blank 16 neg 5 mcL_Y-F9_1_3430.mzXML
SUBJECT_SAMPLE_FACTORS           	-	Blank438	Injection order:143 | Sample source:blank | Site:no | Month:no | season:no | year:no | Temp:no | Fractiontype:no	RAW_FILE_NAME(filename)=Blank 17 neg 5 mcL_Y-F9_1_3438.mzXML
SUBJECT_SAMPLE_FACTORS           	-	Blank446	Injection order:150 | Sample source:blank | Site:no | Month:no | season:no | year:no | Temp:no | Fractiontype:no	RAW_FILE_NAME(filename)=Blank 18 neg 5 mcL_Y-F9_1_3446.mzXML
SUBJECT_SAMPLE_FACTORS           	-	Blank453	Injection order:157 | Sample source:blank | Site:no | Month:no | season:no | year:no | Temp:no | Fractiontype:no	RAW_FILE_NAME(filename)=Blank 19 neg 5 mcL_Y-F9_1_3453.mzXML
SUBJECT_SAMPLE_FACTORS           	-	Blank280	Injection order:8 | Sample source:blank | Site:no | Month:no | season:no | year:no | Temp:no | Fractiontype:no	RAW_FILE_NAME(filename)=Blank 2 neg 5 mcL_Y-F9_1_3280.mzXML
SUBJECT_SAMPLE_FACTORS           	-	Blank287	Injection order:15 | Sample source:blank | Site:no | Month:no | season:no | year:no | Temp:no | Fractiontype:no	RAW_FILE_NAME(filename)=Blank 3 neg 5 mcL_Y-F9_1_3287.mzXML
SUBJECT_SAMPLE_FACTORS           	-	Blank295	Injection order:22 | Sample source:blank | Site:no | Month:no | season:no | year:no | Temp:no | Fractiontype:no	RAW_FILE_NAME(filename)=Blank 4 neg 5 mcL_Y-F9_1_3295.mzXML
SUBJECT_SAMPLE_FACTORS           	-	Blank302	Injection order:29 | Sample source:blank | Site:no | Month:no | season:no | year:no | Temp:no | Fractiontype:no	RAW_FILE_NAME(filename)=Blank 5 neg 5 mcL_Y-F9_1_3302.mzXML
SUBJECT_SAMPLE_FACTORS           	-	Blank309	Injection order:36 | Sample source:blank | Site:no | Month:no | season:no | year:no | Temp:no | Fractiontype:no	RAW_FILE_NAME(filename)=Blank 6 neg 5 mcL_Y-F9_1_3309.mzXML
SUBJECT_SAMPLE_FACTORS           	-	Blank317	Injection order:44 | Sample source:blank | Site:no | Month:no | season:no | year:no | Temp:no | Fractiontype:no	RAW_FILE_NAME(filename)=Blank 7 neg 5 mcL_Y-F9_1_3317.mzXML
SUBJECT_SAMPLE_FACTORS           	-	Blank324	Injection order:51 | Sample source:blank | Site:no | Month:no | season:no | year:no | Temp:no | Fractiontype:no	RAW_FILE_NAME(filename)=Blank 8 neg 5 mcL_Y-F9_1_3324.mzXML
SUBJECT_SAMPLE_FACTORS           	-	Blank331	Injection order:58 | Sample source:blank | Site:no | Month:no | season:no | year:no | Temp:no | Fractiontype:no	RAW_FILE_NAME(filename)=Blank 9 neg 5 mcL_Y-F9_1_3331.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F100	Injection order:74 | Sample source:sample | Site:Shikmona | Month:6 | season:Summer | year:2023 | Temp:28 | Fractiontype:B'	RAW_FILE_NAME(filename)=F100 1 mg-ml ACN_R-F2_1_3348.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F101	Injection order:75 | Sample source:sample | Site:Shikmona | Month:6 | season:Summer | year:2023 | Temp:28 | Fractiontype:D'	RAW_FILE_NAME(filename)=F101 1 mg-ml ACN_R-F3_1_3349.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F102	Injection order:76 | Sample source:sample | Site:Shikmona | Month:6 | season:Summer | year:2023 | Temp:28 | Fractiontype:F'	RAW_FILE_NAME(filename)=F102 1 mg-ml ACN_R-F4_1_3350.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F103	Injection order:77 | Sample source:sample | Site:Shikmona | Month:6 | season:Summer | year:2023 | Temp:28 | Fractiontype:H'	RAW_FILE_NAME(filename)=F103 1 mg-ml ACN_R-F5_1_3351.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F104	Injection order:78 | Sample source:sample | Site:Shikmona | Month:6 | season:Summer | year:2023 | Temp:28 | Fractiontype:I'	RAW_FILE_NAME(filename)=F104 1 mg-ml ACN_R-F6_1_3352.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F105	Injection order:52 | Sample source:sample | Site:Shikmona | Month:12 | season:Winter | year:2022 | Temp:21 | Fractiontype:Crude	RAW_FILE_NAME(filename)=F105 SH201222 1 mg-ml ACN_R-D7_1_3325.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F107	Injection order:53 | Sample source:sample | Site:Shikmona | Month:12 | season:Winter | year:2022 | Temp:21 | Fractiontype:B'	RAW_FILE_NAME(filename)=F107 1 mg-ml ACN_R-D8_1_3326.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F108	Injection order:54 | Sample source:sample | Site:Shikmona | Month:12 | season:Winter | year:2022 | Temp:21 | Fractiontype:D'	RAW_FILE_NAME(filename)=F108 1 mg-ml ACN_R-D9_1_3327.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F109	Injection order:55 | Sample source:sample | Site:Shikmona | Month:12 | season:Winter | year:2022 | Temp:21 | Fractiontype:F'	RAW_FILE_NAME(filename)=F109 1 mg-ml ACN_R-D10_1_3328.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F110	Injection order:56 | Sample source:sample | Site:Shikmona | Month:12 | season:Winter | year:2022 | Temp:21 | Fractiontype:H'	RAW_FILE_NAME(filename)=F110 1 mg-ml ACN_R-D11_1_3329.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F111	Injection order:57 | Sample source:sample | Site:Shikmona | Month:12 | season:Winter | year:2022 | Temp:21 | Fractiontype:I'	RAW_FILE_NAME(filename)=F111 1 mg-ml ACN_R-D12_1_3330.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F112	Injection order:37 | Sample source:sample | Site:Shikmona | Month:8 | season:Summer | year:2022 | Temp:29 | Fractiontype:Crude	RAW_FILE_NAME(filename)=F112 SH020822 1 mg-ml ACN_R-C7_1_3310.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F114	Injection order:38 | Sample source:sample | Site:Shikmona | Month:8 | season:Summer | year:2022 | Temp:29 | Fractiontype:B'	RAW_FILE_NAME(filename)=F114 1 mg-ml ACN_R-C8_1_3311.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F115	Injection order:39 | Sample source:sample | Site:Shikmona | Month:8 | season:Summer | year:2022 | Temp:29 | Fractiontype:D'	RAW_FILE_NAME(filename)=F115 1 mg-ml ACN_R-C9_1_3312.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F116	Injection order:40 | Sample source:sample | Site:Shikmona | Month:8 | season:Summer | year:2022 | Temp:29 | Fractiontype:F'	RAW_FILE_NAME(filename)=F116 1 mg-ml ACN_R-C10_1_3313.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F117	Injection order:41 | Sample source:sample | Site:Shikmona | Month:8 | season:Summer | year:2022 | Temp:29 | Fractiontype:H'	RAW_FILE_NAME(filename)=F117 1 mg-ml ACN_R-C11_1_3314.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F118	Injection order:42 | Sample source:sample | Site:Shikmona | Month:8 | season:Summer | year:2022 | Temp:29 | Fractiontype:I'	RAW_FILE_NAME(filename)=F118 1 mg-ml ACN_R-C12_1_3315.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F119	Injection order:59 | Sample source:sample | Site:Shikmona | Month:2 | season:Winter | year:2023 | Temp:17 | Fractiontype:Crude	RAW_FILE_NAME(filename)=F119 SH190223 1 mg-ml ACN_R-E1_1_3332.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F121	Injection order:60 | Sample source:sample | Site:Shikmona | Month:2 | season:Winter | year:2023 | Temp:17 | Fractiontype:B'	RAW_FILE_NAME(filename)=F121 1 mg-ml ACN_R-E2_1_3333.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F122	Injection order:61 | Sample source:sample | Site:Shikmona | Month:2 | season:Winter | year:2023 | Temp:17 | Fractiontype:D'	RAW_FILE_NAME(filename)=F122 1 mg-ml ACN_R-E3_1_3334.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F123	Injection order:62 | Sample source:sample | Site:Shikmona | Month:2 | season:Winter | year:2023 | Temp:17 | Fractiontype:F'	RAW_FILE_NAME(filename)=F123 1 mg-ml ACN_R-E4_1_3335.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F124	Injection order:63 | Sample source:sample | Site:Shikmona | Month:2 | season:Winter | year:2023 | Temp:17 | Fractiontype:H'	RAW_FILE_NAME(filename)=F124 1 mg-ml ACN_R-E5_1_3336.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F125	Injection order:64 | Sample source:sample | Site:Shikmona | Month:2 | season:Winter | year:2023 | Temp:17 | Fractiontype:I'	RAW_FILE_NAME(filename)=F125 1 mg-ml ACN_R-E6_1_3337.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F126	Injection order:136 | Sample source:sample | Site:Bat galim | Month:12 | season:Winter | year:2022 | Temp:21 | Fractiontype:Crude	RAW_FILE_NAME(filename)=F126 BG251222 1 mg-ml ACN_R-D1_1_3431.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F128	Injection order:137 | Sample source:sample | Site:Bat galim | Month:12 | season:Winter | year:2022 | Temp:21 | Fractiontype:B'	RAW_FILE_NAME(filename)=F128 1 mg-ml ACN_R-D2_1_3432.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F129	Injection order:138 | Sample source:sample | Site:Bat galim | Month:12 | season:Winter | year:2022 | Temp:21 | Fractiontype:D'	RAW_FILE_NAME(filename)=F129 1 mg-ml ACN_R-D3_1_3433.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F130	Injection order:139 | Sample source:sample | Site:Bat galim | Month:12 | season:Winter | year:2022 | Temp:21 | Fractiontype:F'	RAW_FILE_NAME(filename)=F130 1 mg-ml ACN_R-D4_1_3434.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F131	Injection order:140 | Sample source:sample | Site:Bat galim | Month:12 | season:Winter | year:2022 | Temp:21 | Fractiontype:H'	RAW_FILE_NAME(filename)=F131 1 mg-ml ACN_R-D5_1_3435.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F132	Injection order:141 | Sample source:sample | Site:Bat galim | Month:12 | season:Winter | year:2022 | Temp:21 | Fractiontype:I'	RAW_FILE_NAME(filename)=F132 1 mg-ml ACN_R-D6_1_3436.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F133	Injection order:45 | Sample source:sample | Site:Shikmona | Month:10 | season:Fall | year:2022 | Temp:26 | Fractiontype:Crude	RAW_FILE_NAME(filename)=F133 SH251022 1 mg-ml ACN_R-D1_1_3318.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F134	Injection order:46 | Sample source:sample | Site:Shikmona | Month:10 | season:Fall | year:2022 | Temp:26 | Fractiontype:B'	RAW_FILE_NAME(filename)=F134 1 mg-ml ACN_R-D2_1_3319.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F135	Injection order:47 | Sample source:sample | Site:Shikmona | Month:10 | season:Fall | year:2022 | Temp:26 | Fractiontype:D'	RAW_FILE_NAME(filename)=F135 1 mg-ml ACN_R-D3_1_3320.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F136	Injection order:48 | Sample source:sample | Site:Shikmona | Month:10 | season:Fall | year:2022 | Temp:26 | Fractiontype:F'	RAW_FILE_NAME(filename)=F136 1 mg-ml ACN_R-D4_1_3321.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F137	Injection order:49 | Sample source:sample | Site:Shikmona | Month:10 | season:Fall | year:2022 | Temp:26 | Fractiontype:H'	RAW_FILE_NAME(filename)=F137 1 mg-ml ACN_R-D5_1_3322.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F138	Injection order:50 | Sample source:sample | Site:Shikmona | Month:10 | season:Fall | year:2022 | Temp:26 | Fractiontype:I'	RAW_FILE_NAME(filename)=F138 1 mg-ml ACN_R-D6_1_3323.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F140	Injection order:66 | Sample source:sample | Site:Shikmona | Month:4 | season:Spring | year:2023 | Temp:21 | Fractiontype:Crude	RAW_FILE_NAME(filename)=F140 SH240423 1 mg-ml ACN_R-E7_1_3340.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F142	Injection order:67 | Sample source:sample | Site:Shikmona | Month:4 | season:Spring | year:2023 | Temp:21 | Fractiontype:B'	RAW_FILE_NAME(filename)=F142 1 mg-ml ACN_R-E8_1_3341.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F143	Injection order:68 | Sample source:sample | Site:Shikmona | Month:4 | season:Spring | year:2023 | Temp:21 | Fractiontype:D'	RAW_FILE_NAME(filename)=F143 1 mg-ml ACN_R-E9_1_3342.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F144	Injection order:69 | Sample source:sample | Site:Shikmona | Month:4 | season:Spring | year:2023 | Temp:21 | Fractiontype:F'	RAW_FILE_NAME(filename)=F144 1 mg-ml ACN_R-E10_1_3343.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F145	Injection order:70 | Sample source:sample | Site:Shikmona | Month:4 | season:Spring | year:2023 | Temp:21 | Fractiontype:H'	RAW_FILE_NAME(filename)=F145 1 mg-ml ACN_R-E11_1_3344.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F146	Injection order:71 | Sample source:sample | Site:Shikmona | Month:4 | season:Spring | year:2023 | Temp:21 | Fractiontype:I'	RAW_FILE_NAME(filename)=F146 1 mg-ml ACN_R-E12_1_3345.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F147	Injection order:95 | Sample source:sample | Site:Shikmona | Month:10 | season:Fall | year:2022 | Temp:26 | Fractiontype:Crude	RAW_FILE_NAME(filename)=F147 SHDSD251021 1 mg-ml ACN_R-G7_1_3370.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F148	Injection order:96 | Sample source:sample | Site:Shikmona | Month:10 | season:Fall | year:2022 | Temp:26 | Fractiontype:Crude	RAW_FILE_NAME(filename)=F148 SHDSM251021 1 mg-ml ACN_R-G8_1_3371.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F149	Injection order:97 | Sample source:sample | Site:Shikmona | Month:10 | season:Fall | year:2022 | Temp:26 | Fractiontype:Crude	RAW_FILE_NAME(filename)=F149 SHDSU251021 1 mg-ml ACN_R-G9_1_3372.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F157	Injection order:129 | Sample source:sample | Site:Bat galim | Month:10 | season:Fall | year:2022 | Temp:26 | Fractiontype:Crude	RAW_FILE_NAME(filename)=F157 BG261022 1 mg-ml ACN_R-C7_1_3424.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F159	Injection order:130 | Sample source:sample | Site:Bat galim | Month:10 | season:Fall | year:2022 | Temp:26 | Fractiontype:B'	RAW_FILE_NAME(filename)=F159 1 mg-ml ACN_R-C8_1_3425.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F160	Injection order:131 | Sample source:sample | Site:Bat galim | Month:10 | season:Fall | year:2022 | Temp:26 | Fractiontype:D'	RAW_FILE_NAME(filename)=F160 1 mg-ml ACN_R-C9_1_3426.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F161	Injection order:132 | Sample source:sample | Site:Bat galim | Month:10 | season:Fall | year:2022 | Temp:26 | Fractiontype:F'	RAW_FILE_NAME(filename)=F161 1 mg-ml ACN_R-C10_1_3427.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F162	Injection order:133 | Sample source:sample | Site:Bat galim | Month:10 | season:Fall | year:2022 | Temp:26 | Fractiontype:H'	RAW_FILE_NAME(filename)=F162 1 mg-ml ACN_R-C11_1_3428.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F163	Injection order:134 | Sample source:sample | Site:Bat galim | Month:10 | season:Fall | year:2022 | Temp:26 | Fractiontype:I'	RAW_FILE_NAME(filename)=F163 1 mg-ml ACN_R-C12_1_3429.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F164	Injection order:122 | Sample source:sample | Site:Bat galim | Month:8 | season:Summer | year:2022 | Temp:30 | Fractiontype:Crude	RAW_FILE_NAME(filename)=F164 BG100822 1 mg-ml ACN_R-C1_1_3417.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F166	Injection order:123 | Sample source:sample | Site:Bat galim | Month:8 | season:Summer | year:2022 | Temp:30 | Fractiontype:B'	RAW_FILE_NAME(filename)=F166 1 mg-ml ACN_R-C2_1_3418.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F167	Injection order:124 | Sample source:sample | Site:Bat galim | Month:8 | season:Summer | year:2022 | Temp:30 | Fractiontype:D'	RAW_FILE_NAME(filename)=F167 1 mg-ml ACN_R-C3_1_3419.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F168	Injection order:125 | Sample source:sample | Site:Bat galim | Month:8 | season:Summer | year:2022 | Temp:30 | Fractiontype:F'	RAW_FILE_NAME(filename)=F168 1 mg-ml ACN_R-C4_1_3420.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F169	Injection order:126 | Sample source:sample | Site:Bat galim | Month:8 | season:Summer | year:2022 | Temp:30 | Fractiontype:H'	RAW_FILE_NAME(filename)=F169 1 mg-ml ACN_R-C5_1_3421.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F170	Injection order:127 | Sample source:sample | Site:Bat galim | Month:8 | season:Summer | year:2022 | Temp:30 | Fractiontype:I'	RAW_FILE_NAME(filename)=F170 1 mg-ml ACN_R-C6_1_3422.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F171	Injection order:144 | Sample source:sample | Site:Bat galim | Month:2 | season:Winter | year:2023 | Temp:17 | Fractiontype:Crude	RAW_FILE_NAME(filename)=F171 BG180223 1 mg-ml ACN_R-D7_1_3439.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F173	Injection order:145 | Sample source:sample | Site:Bat galim | Month:2 | season:Winter | year:2023 | Temp:17 | Fractiontype:B'	RAW_FILE_NAME(filename)=F173 1 mg-ml ACN_R-D8_1_3440.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F174	Injection order:146 | Sample source:sample | Site:Bat galim | Month:2 | season:Winter | year:2023 | Temp:17 | Fractiontype:D'	RAW_FILE_NAME(filename)=F174 1 mg-ml ACN_R-D9_1_3441.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F175	Injection order:147 | Sample source:sample | Site:Bat galim | Month:2 | season:Winter | year:2023 | Temp:17 | Fractiontype:F'	RAW_FILE_NAME(filename)=F175 1 mg-ml ACN_R-D10_1_3442.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F176	Injection order:148 | Sample source:sample | Site:Bat galim | Month:2 | season:Winter | year:2023 | Temp:17 | Fractiontype:H'	RAW_FILE_NAME(filename)=F176 1 mg-ml ACN_R-D11_1_3443.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F177	Injection order:149 | Sample source:sample | Site:Bat galim | Month:2 | season:Winter | year:2023 | Temp:17 | Fractiontype:I'	RAW_FILE_NAME(filename)=F177 1 mg-ml ACN_R-D12_1_3444.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F178	Injection order:158 | Sample source:sample | Site:Bat galim | Month:6 | season:Summer | year:2023 | Temp:28 | Fractiontype:Crude	RAW_FILE_NAME(filename)=F178 BG050723 1 mg-ml ACN_R-E7_1_3454.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F180	Injection order:159 | Sample source:sample | Site:Bat galim | Month:6 | season:Summer | year:2023 | Temp:28 | Fractiontype:B'	RAW_FILE_NAME(filename)=F180 1 mg-ml ACN_R-E8_1_3455.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F181	Injection order:160 | Sample source:sample | Site:Bat galim | Month:6 | season:Summer | year:2023 | Temp:28 | Fractiontype:D'	RAW_FILE_NAME(filename)=F181 1 mg-ml ACN_R-E9_1_3456.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F182	Injection order:161 | Sample source:sample | Site:Bat galim | Month:6 | season:Summer | year:2023 | Temp:28 | Fractiontype:F'	RAW_FILE_NAME(filename)=F182 1 mg-ml ACN_R-E10_1_3457.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F183	Injection order:162 | Sample source:sample | Site:Bat galim | Month:6 | season:Summer | year:2023 | Temp:28 | Fractiontype:H'	RAW_FILE_NAME(filename)=F183 1 mg-ml ACN_R-E11_1_3458.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F184	Injection order:163 | Sample source:sample | Site:Bat galim | Month:6 | season:Summer | year:2023 | Temp:28 | Fractiontype:I'	RAW_FILE_NAME(filename)=F184 1 mg-ml ACN_R-E12_1_3459.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F185	Injection order:165 | Sample source:sample | Site:Bat galim | Month:6 | season:Summer | year:2022 | Temp:24 | Fractiontype:Crude	RAW_FILE_NAME(filename)=F185 BG060621 1 mg-ml ACN_R-F1_1_3462.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F28	Injection order:2 | Sample source:sample | Site:Shikmona | Month:8 | season:Summer | year:2021 | Temp:29 | Fractiontype:Crude	RAW_FILE_NAME(filename)=F28 SH260821 1 mg-ml ACN_R-A1_1_3274.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F30	Injection order:3 | Sample source:sample | Site:Shikmona | Month:8 | season:Summer | year:2021 | Temp:29 | Fractiontype:B'	RAW_FILE_NAME(filename)=F30 1 mg-ml ACN_R-A2_1_3275.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F31	Injection order:4 | Sample source:sample | Site:Shikmona | Month:8 | season:Summer | year:2021 | Temp:29 | Fractiontype:D'	RAW_FILE_NAME(filename)=F31 1 mg-ml ACN_R-A3_1_3276.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F32	Injection order:5 | Sample source:sample | Site:Shikmona | Month:8 | season:Summer | year:2021 | Temp:29 | Fractiontype:F'	RAW_FILE_NAME(filename)=F32 1 mg-ml ACN_R-A4_1_3277.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F33	Injection order:6 | Sample source:sample | Site:Shikmona | Month:8 | season:Summer | year:2021 | Temp:29 | Fractiontype:H'	RAW_FILE_NAME(filename)=F33 1 mg-ml ACN_R-A5_1_3278.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F34	Injection order:7 | Sample source:sample | Site:Shikmona | Month:8 | season:Summer | year:2021 | Temp:29 | Fractiontype:I'	RAW_FILE_NAME(filename)=F34 1 mg-ml ACN_R-A6_1_3279.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F35	Injection order:9 | Sample source:sample | Site:Shikmona | Month:10 | season:Fall | year:2021 | Temp:27 | Fractiontype:Crude	RAW_FILE_NAME(filename)=F35 SH161021 1 mg-ml ACN_R-A7_1_3281.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F37	Injection order:10 | Sample source:sample | Site:Shikmona | Month:10 | season:Fall | year:2021 | Temp:27 | Fractiontype:B'	RAW_FILE_NAME(filename)=F37 1 mg-ml ACN_R-A8_1_3282.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F38	Injection order:11 | Sample source:sample | Site:Shikmona | Month:10 | season:Fall | year:2021 | Temp:27 | Fractiontype:D'	RAW_FILE_NAME(filename)=F38 1 mg-ml ACN_R-A9_1_3283.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F39	Injection order:12 | Sample source:sample | Site:Shikmona | Month:10 | season:Fall | year:2021 | Temp:27 | Fractiontype:F'	RAW_FILE_NAME(filename)=F39 1 mg-ml ACN_R-A10_1_3284.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F40	Injection order:13 | Sample source:sample | Site:Shikmona | Month:10 | season:Fall | year:2021 | Temp:27 | Fractiontype:H'	RAW_FILE_NAME(filename)=F40 1 mg-ml ACN_R-A11_1_3285.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F41	Injection order:14 | Sample source:sample | Site:Shikmona | Month:10 | season:Fall | year:2021 | Temp:27 | Fractiontype:I'	RAW_FILE_NAME(filename)=F41 1 mg-ml ACN_R-A12_1_3286.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F42	Injection order:107 | Sample source:sample | Site:Bat galim | Month:10 | season:Fall | year:2021 | Temp:27 | Fractiontype:Crude	RAW_FILE_NAME(filename)=F42 BG241021 1 mg-ml ACN_R-B1_1_3402.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F44	Injection order:108 | Sample source:sample | Site:Bat galim | Month:10 | season:Fall | year:2021 | Temp:27 | Fractiontype:B'	RAW_FILE_NAME(filename)=F44 1 mg-ml ACN_R-B2_1_3403.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F45	Injection order:109 | Sample source:sample | Site:Bat galim | Month:10 | season:Fall | year:2021 | Temp:27 | Fractiontype:D'	RAW_FILE_NAME(filename)=F45 1 mg-ml ACN_R-B3_1_3404.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F46	Injection order:110 | Sample source:sample | Site:Bat galim | Month:10 | season:Fall | year:2021 | Temp:27 | Fractiontype:F'	RAW_FILE_NAME(filename)=F46 1 mg-ml ACN_R-B4_1_3405.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F47	Injection order:111 | Sample source:sample | Site:Bat galim | Month:10 | season:Fall | year:2021 | Temp:27 | Fractiontype:H'	RAW_FILE_NAME(filename)=F47 1 mg-ml ACN_R-B5_1_3406.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F48	Injection order:112 | Sample source:sample | Site:Bat galim | Month:10 | season:Fall | year:2021 | Temp:27 | Fractiontype:I'	RAW_FILE_NAME(filename)=F48 1 mg-ml ACN_R-B6_1_3407.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F49	Injection order:100 | Sample source:sample | Site:Bat galim | Month:8 | season:Summer | year:2021 | Temp:29 | Fractiontype:Crude	RAW_FILE_NAME(filename)=F49 BG040821 1 mg-ml ACN_R-A7_1_3395.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F51	Injection order:101 | Sample source:sample | Site:Bat galim | Month:8 | season:Summer | year:2021 | Temp:29 | Fractiontype:B'	RAW_FILE_NAME(filename)=F51 1 mg-ml ACN_R-A8_1_3396.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F52	Injection order:102 | Sample source:sample | Site:Bat galim | Month:8 | season:Summer | year:2021 | Temp:29 | Fractiontype:D'	RAW_FILE_NAME(filename)=F52 1 mg-ml ACN_R-A9_1_3397.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F53	Injection order:103 | Sample source:sample | Site:Bat galim | Month:8 | season:Summer | year:2021 | Temp:29 | Fractiontype:F'	RAW_FILE_NAME(filename)=F53 1 mg-ml ACN_R-A10_1_3398.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F54	Injection order:104 | Sample source:sample | Site:Bat galim | Month:8 | season:Summer | year:2021 | Temp:29 | Fractiontype:H'	RAW_FILE_NAME(filename)=F54 1 mg-ml ACN_R-A11_1_3399.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F55	Injection order:105 | Sample source:sample | Site:Bat galim | Month:8 | season:Summer | year:2021 | Temp:29 | Fractiontype:I'	RAW_FILE_NAME(filename)=F55 1 mg-ml ACN_R-A12_1_3400.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F56	Injection order:80 | Sample source:sample | Site:Rosh Hanikra | Month:10 | season:Fall | year:2021 | Temp:27 | Fractiontype:Crude	RAW_FILE_NAME(filename)=F56 RH251021 1 mg-ml ACN_R-F7_1_3354.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F58	Injection order:81 | Sample source:sample | Site:Rosh Hanikra | Month:10 | season:Fall | year:2021 | Temp:27 | Fractiontype:B'	RAW_FILE_NAME(filename)=F58 1 mg-ml ACN_R-F8_1_3355.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F59	Injection order:82 | Sample source:sample | Site:Rosh Hanikra | Month:10 | season:Fall | year:2021 | Temp:27 | Fractiontype:D'	RAW_FILE_NAME(filename)=F59 1 mg-ml ACN_R-F9_1_3356.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F60	Injection order:83 | Sample source:sample | Site:Rosh Hanikra | Month:10 | season:Fall | year:2021 | Temp:27 | Fractiontype:F'	RAW_FILE_NAME(filename)=F60 1 mg-ml ACN_R-F10_1_3357.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F61	Injection order:84 | Sample source:sample | Site:Rosh Hanikra | Month:10 | season:Fall | year:2021 | Temp:27 | Fractiontype:H'	RAW_FILE_NAME(filename)=F61 1 mg-ml ACN_R-F11_1_3358.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F62	Injection order:85 | Sample source:sample | Site:Rosh Hanikra | Month:10 | season:Fall | year:2021 | Temp:27 | Fractiontype:I'	RAW_FILE_NAME(filename)=F62 1 mg-ml ACN_R-F12_1_3359.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F63	Injection order:23 | Sample source:sample | Site:Shikmona | Month:4 | season:Spring | year:2022 | Temp:18 | Fractiontype:Crude	RAW_FILE_NAME(filename)=F63 SH230322 1 mg-ml ACN_R-B7_1_3296.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F65	Injection order:24 | Sample source:sample | Site:Shikmona | Month:4 | season:Spring | year:2022 | Temp:18 | Fractiontype:B'	RAW_FILE_NAME(filename)=F65 1 mg-ml ACN_R-B8_1_3297.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F66	Injection order:25 | Sample source:sample | Site:Shikmona | Month:4 | season:Spring | year:2022 | Temp:18 | Fractiontype:D'	RAW_FILE_NAME(filename)=F66 1 mg-ml ACN_R-B9_1_3298.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F67	Injection order:26 | Sample source:sample | Site:Shikmona | Month:4 | season:Spring | year:2022 | Temp:18 | Fractiontype:F'	RAW_FILE_NAME(filename)=F67 1 mg-ml ACN_R-B10_1_3299.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F68	Injection order:27 | Sample source:sample | Site:Shikmona | Month:4 | season:Spring | year:2022 | Temp:18 | Fractiontype:H'	RAW_FILE_NAME(filename)=F68 1 mg-ml ACN_R-B11_1_3300.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F69	Injection order:28 | Sample source:sample | Site:Shikmona | Month:4 | season:Spring | year:2022 | Temp:18 | Fractiontype:I'	RAW_FILE_NAME(filename)=F69 1 mg-ml ACN_R-B12_1_3301.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F70	Injection order:30 | Sample source:sample | Site:Shikmona | Month:6 | season:Summer | year:2022 | Temp:24 | Fractiontype:Crude	RAW_FILE_NAME(filename)=F70 SH310522 1 mg-ml ACN_R-C1_1_3303.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F72	Injection order:31 | Sample source:sample | Site:Shikmona | Month:6 | season:Summer | year:2022 | Temp:24 | Fractiontype:B'	RAW_FILE_NAME(filename)=F72 1 mg-ml ACN_R-C2_1_3304.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F73	Injection order:32 | Sample source:sample | Site:Shikmona | Month:6 | season:Summer | year:2022 | Temp:24 | Fractiontype:D'	RAW_FILE_NAME(filename)=F73 1 mg-ml ACN_R-C3_1_3305.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F74	Injection order:33 | Sample source:sample | Site:Shikmona | Month:6 | season:Summer | year:2022 | Temp:24 | Fractiontype:F'	RAW_FILE_NAME(filename)=F74 1 mg-ml ACN_R-C4_1_3306.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F75	Injection order:34 | Sample source:sample | Site:Shikmona | Month:6 | season:Summer | year:2022 | Temp:24 | Fractiontype:H'	RAW_FILE_NAME(filename)=F75 1 mg-ml ACN_R-C5_1_3307.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F76	Injection order:35 | Sample source:sample | Site:Shikmona | Month:6 | season:Summer | year:2022 | Temp:24 | Fractiontype:I'	RAW_FILE_NAME(filename)=F76 1 mg-ml ACN_R-C6_1_3308.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F77	Injection order:16 | Sample source:sample | Site:Shikmona | Month:12 | season:Winter | year:2021 | Temp:20 | Fractiontype:Crude	RAW_FILE_NAME(filename)=F77 SH291221 1 mg-ml ACN_R-B1_1_3288.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F79	Injection order:17 | Sample source:sample | Site:Shikmona | Month:12 | season:Winter | year:2021 | Temp:20 | Fractiontype:B'	RAW_FILE_NAME(filename)=F79 1 mg-ml ACN_R-B2_1_3289.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F80	Injection order:18 | Sample source:sample | Site:Shikmona | Month:12 | season:Winter | year:2021 | Temp:20 | Fractiontype:D'	RAW_FILE_NAME(filename)=F80 1 mg-ml ACN_R-B3_1_3290.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F81	Injection order:19 | Sample source:sample | Site:Shikmona | Month:12 | season:Winter | year:2021 | Temp:20 | Fractiontype:F'	RAW_FILE_NAME(filename)=F81 1 mg-ml ACN_R-B4_1_3291.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F82	Injection order:20 | Sample source:sample | Site:Shikmona | Month:12 | season:Winter | year:2021 | Temp:20 | Fractiontype:H'	RAW_FILE_NAME(filename)=F82 1 mg-ml ACN_R-B5_1_3292.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F83	Injection order:21 | Sample source:sample | Site:Shikmona | Month:12 | season:Winter | year:2021 | Temp:20 | Fractiontype:I'	RAW_FILE_NAME(filename)=F83 1 mg-ml ACN_R-B6_1_3293.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F84	Injection order:114 | Sample source:sample | Site:Bat galim | Month:12 | season:Winter | year:2021 | Temp:20 | Fractiontype:Crude	RAW_FILE_NAME(filename)=F84 BG271221 1 mg-ml ACN_R-B7_1_3409.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F86	Injection order:115 | Sample source:sample | Site:Bat galim | Month:12 | season:Winter | year:2021 | Temp:20 | Fractiontype:B'	RAW_FILE_NAME(filename)=F86 1 mg-ml ACN_R-B8_1_3410.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F87	Injection order:116 | Sample source:sample | Site:Bat galim | Month:12 | season:Winter | year:2021 | Temp:20 | Fractiontype:D'	RAW_FILE_NAME(filename)=F87 1 mg-ml ACN_R-B9_1_3411.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F88	Injection order:117 | Sample source:sample | Site:Bat galim | Month:12 | season:Winter | year:2021 | Temp:20 | Fractiontype:F'	RAW_FILE_NAME(filename)=F88 1 mg-ml ACN_R-B10_1_3412.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F89	Injection order:118 | Sample source:sample | Site:Bat galim | Month:12 | season:Winter | year:2021 | Temp:20 | Fractiontype:H'	RAW_FILE_NAME(filename)=F89 1 mg-ml ACN_R-B11_1_3413.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F90	Injection order:119 | Sample source:sample | Site:Bat galim | Month:12 | season:Winter | year:2021 | Temp:20 | Fractiontype:I'	RAW_FILE_NAME(filename)=F90 1 mg-ml ACN_R-B12_1_3414.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F91	Injection order:151 | Sample source:sample | Site:Bat galim | Month:4 | season:Spring | year:2023 | Temp:22 | Fractiontype:Crude	RAW_FILE_NAME(filename)=F91 BG110523 1 mg-ml ACN_R-E1_1_3447.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F93	Injection order:152 | Sample source:sample | Site:Bat galim | Month:4 | season:Spring | year:2023 | Temp:22 | Fractiontype:B'	RAW_FILE_NAME(filename)=F93 1 mg-ml ACN_R-E2_1_3448.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F94	Injection order:153 | Sample source:sample | Site:Bat galim | Month:4 | season:Spring | year:2023 | Temp:22 | Fractiontype:D'	RAW_FILE_NAME(filename)=F94 1 mg-ml ACN_R-E3_1_3449.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F95	Injection order:154 | Sample source:sample | Site:Bat galim | Month:4 | season:Spring | year:2023 | Temp:22 | Fractiontype:F'	RAW_FILE_NAME(filename)=F95 1 mg-ml ACN_R-E4_1_3450.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F96	Injection order:155 | Sample source:sample | Site:Bat galim | Month:4 | season:Spring | year:2023 | Temp:22 | Fractiontype:H'	RAW_FILE_NAME(filename)=F96 1 mg-ml ACN_R-E5_1_3451.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F97	Injection order:156 | Sample source:sample | Site:Bat galim | Month:4 | season:Spring | year:2023 | Temp:22 | Fractiontype:I'	RAW_FILE_NAME(filename)=F97 1 mg-ml ACN_R-E6_1_3452.mzXML
SUBJECT_SAMPLE_FACTORS           	-	F98	Injection order:73 | Sample source:sample | Site:no | Month:no | season:no | year:no | Temp:no | Fractiontype:no	RAW_FILE_NAME(filename)=F98 SH040723 1 mg-ml ACN_R-F1_1_3347.mzXML
SUBJECT_SAMPLE_FACTORS           	-	Wash_3316	Injection order:43 | Sample source:Wash | Site:no | Month:no | season:no | year:no | Temp:no | Fractiontype:no	RAW_FILE_NAME(filename)=Wash_Y-A9_1_3316.mzXML
SUBJECT_SAMPLE_FACTORS           	-	Wash_3360	Injection order:86 | Sample source:Wash | Site:no | Month:no | season:no | year:no | Temp:no | Fractiontype:no	RAW_FILE_NAME(filename)=Wash_Y-A9_1_3360.mzXML
SUBJECT_SAMPLE_FACTORS           	-	Wash_3415	Injection order:120 | Sample source:Wash | Site:no | Month:no | season:no | year:no | Temp:no | Fractiontype:no	RAW_FILE_NAME(filename)=Wash_Y-A9_1_3415.mzXML
SUBJECT_SAMPLE_FACTORS           	-	Wash_3437	Injection order:142 | Sample source:Wash | Site:no | Month:no | season:no | year:no | Temp:no | Fractiontype:no	RAW_FILE_NAME(filename)=Wash_Y-A9_1_3437.mzXML
#COLLECTION
CO:COLLECTION_SUMMARY            	Gametophytes of Asparagopsis taxiformis (n = 72) were collected from two sites
CO:COLLECTION_SUMMARY            	along the northern coast of the Israeli Mediterranean Sea (IMS): Shikmona
CO:COLLECTION_SUMMARY            	(32°49′33.8″N, 34°57′16.8″E), located within a designated marine
CO:COLLECTION_SUMMARY            	protected area (MPA), and Bat Galim (32°50′11.1″N, 34°58′40.6″E),
CO:COLLECTION_SUMMARY            	situated outside the MPA near a popular public beach (Fig. 1). Sampling was
CO:COLLECTION_SUMMARY            	conducted between November 2020 and July 2023, with regular collections
CO:COLLECTION_SUMMARY            	occurring at least bimonthly from October 2021 onward, dependent on the presence
CO:COLLECTION_SUMMARY            	of A. taxiformis at the sites and time. At each sampling point, three different
CO:COLLECTION_SUMMARY            	specimens, situated at least three meters apart, were collected and rubbed with
CO:COLLECTION_SUMMARY            	a swab immediately after being removed from the water. The swab was then placed
CO:COLLECTION_SUMMARY            	in 1 mL of lysis buffer solution (40 mM EDTA, 50 mM Tris pH 8.3, and 0.75 M
CO:COLLECTION_SUMMARY            	sucrose). Additionally, approximately 100 mg of thalli were also added to 1 mL
CO:COLLECTION_SUMMARY            	of lysis buffer solution. Both samples were transported to the laboratory and
CO:COLLECTION_SUMMARY            	stored at -80°C until DNA extraction. For each sampling point, the three
CO:COLLECTION_SUMMARY            	different biomass samples from the various specimens were washed with fresh
CO:COLLECTION_SUMMARY            	water and then stored at -80°C, followed by freeze-drying using a Lyophilizer.
CO:COLLECTION_SUMMARY            	The dry biomass was used for preparing extracts for metabolomic profiling.
CO:SAMPLE_TYPE                   	Gametophyte of algae
CO:STORAGE_CONDITIONS            	Described in summary
#TREATMENT
TR:TREATMENT_SUMMARY             	No treatment.
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	The freeze-dried biomass was weighed and submerged in a dichloromethane (DCM)
SP:SAMPLEPREP_SUMMARY            	and methanol (MeOH) mixture at a 2:1 ratio. The mixture underwent sonication
SP:SAMPLEPREP_SUMMARY            	(Digital Pro- digital ultrasonic cleaner) for 30 minutes to enhance extraction.
SP:SAMPLEPREP_SUMMARY            	After 24 hours, the liquid was drained and set aside. Fresh DCM:MeOH (2:1) was
SP:SAMPLEPREP_SUMMARY            	added to the biomass, and the process was repeated to ensure thorough
SP:SAMPLEPREP_SUMMARY            	extraction. The combined liquids were evaporated using a rotary evaporator to
SP:SAMPLEPREP_SUMMARY            	obtain the Crude. For the resultant dry material, a known amount of DMSO was
SP:SAMPLEPREP_SUMMARY            	added for long-term storage. Alternatively, the material was mixed with DCM:
SP:SAMPLEPREP_SUMMARY            	MeOH (2:1) and silica powder for vacuum liquid chromatography (VLC) preparation.
SP:SAMPLEPREP_SUMMARY            	The loaded silica powder was dried using evaporation and subsequently utilized
SP:SAMPLEPREP_SUMMARY            	for the VLC. The extraction library was prepared using a vacuum pump,
SP:SAMPLEPREP_SUMMARY            	facilitating the following sequential solvents: Fraction B’: 90% Hexane, 10%
SP:SAMPLEPREP_SUMMARY            	Ethyl Acetate, Fraction D’: 60% Hexane, 40% Ethyl Acetate, Fraction F’: 20%
SP:SAMPLEPREP_SUMMARY            	Hexane, 80% Ethyl Acetate, Fraction H’: 75% Ethyl Acetate, 25% Methanol,
SP:SAMPLEPREP_SUMMARY            	Fraction I’: 100% Methanol. Pure MeOH was used as a blank injection.
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_SUMMARY        	Samples were analyzed with Thermo Scientific Vanquish HPLC system coupled with
CH:CHROMATOGRAPHY_SUMMARY        	Bruker TIMS-TOF Pro 2 mass spectrometer. Chromatographic separation was achieved
CH:CHROMATOGRAPHY_SUMMARY        	on a Kinetex C18 column (2.1 × 50 mm, 1.7 μm, Phenomenex, USA) with guard
CH:CHROMATOGRAPHY_SUMMARY        	column. Flow rate was set to 0.3 mL×min−1, column compartment was set to 35
CH:CHROMATOGRAPHY_SUMMARY        	°C, and autosampler tray was maintained at 4 °C. Initial mobile phase A
CH:CHROMATOGRAPHY_SUMMARY        	consisted of water with 0.1% (v/v) formic acid, mobile phase B was acetonitrile
CH:CHROMATOGRAPHY_SUMMARY        	with 0.1% (v/v) formic acid. The mobile phase linear gradient (%B) was as
CH:CHROMATOGRAPHY_SUMMARY        	follows: 0.0-1.0 min 5 %, 7.0 min 95 %, 9.0-10.5 min 95%, 11.0 min 5 %, 15.0 min
CH:CHROMATOGRAPHY_SUMMARY        	5 %. The injection volume was 1 uL.
CH:CHROMATOGRAPHY_TYPE           	Reversed phase
CH:INSTRUMENT_NAME               	Thermo Vanquish
CH:COLUMN_NAME                   	Phenomenex Kinetex C18 (50 x 2.1mm,1.7um)
CH:SOLVENT_A                     	100% water; 0.1% formic acid
CH:SOLVENT_B                     	100% acetonitrile; 0.1% formic acid
CH:FLOW_GRADIENT                 	The mobile phase linear gradient (%B) was as follows: 0.0-1.0 min 5 %, 7.0 min
CH:FLOW_GRADIENT                 	95 %, 9.0-10.5 min 95%, 11.0 min 5 %, 15.0 min 5 %
CH:FLOW_RATE                     	0.3 mL/min
CH:COLUMN_TEMPERATURE            	35
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
#MS
MS:INSTRUMENT_NAME               	Bruker timsTOF
MS:INSTRUMENT_TYPE               	QTOF
MS:MS_TYPE                       	ESI
MS:ION_MODE                      	NEGATIVE
MS:MS_COMMENTS                   	Mass detection has been performed via timsTOF Pro2 instrument with an ESI
MS:MS_COMMENTS                   	ionization source working in negative mode in SCAN and DDA (Auto MS/MS) modes
MS:MS_COMMENTS                   	simultaneously. Metabolites were recorded in the range 50-2000 m/z. DDA
MS:MS_COMMENTS                   	parameters were following: MS spectra rate 1 Hz, MS/MS spectra rate 12 Hz, MS/MS
MS:MS_COMMENTS                   	spectra rate limits 12-20 Hz, rate control: Dynamic, total cycle time 1.83 s,
MS:MS_COMMENTS                   	target intensity 20 000, preferred charge states: 1-2, number of precursors: 10,
MS:MS_COMMENTS                   	active exclusion after 3 spectra and release after 0.5 min. Ionization source
MS:MS_COMMENTS                   	parameters were following: nebulizer 2.2 Bar, dry gas 10 L/min, capillary
MS:MS_COMMENTS                   	voltage 4.5 kV, dry temperature 300 °C. Accumulation time was set to 200 ms,
MS:MS_COMMENTS                   	resolution – 70K; automatic gain control – 5E+06.
MS:MS_RESULTS_FILE               	ST004265_AN007098_Results.txt	UNITS:Area	Has m/z:Yes	Has RT:Yes	RT units:Minutes
#END