#METABOLOMICS WORKBENCH katherine_heal_20251027_151419 DATATRACK_ID:6603 STUDY_ID:ST004324 ANALYSIS_ID:AN007207 PROJECT_ID:PR002738 VERSION 1 CREATED_ON 11-06-2025 #PROJECT PR:PROJECT_TITLE Conserved pathway for homarine catabolism in environmental bacteria PR:PROJECT_SUMMARY Homarine (N-methylpicolinic acid) is a ubiquitous marine metabolite produced by PR:PROJECT_SUMMARY phytoplankton and noted for its bioactivity in marine animals, yet its microbial PR:PROJECT_SUMMARY degradation pathways are uncharacterized. Here, we identify a conserved operon PR:PROJECT_SUMMARY (homABCDER) that mediates homarine catabolism in bacteria using comparative PR:PROJECT_SUMMARY transcriptomics, mutagenesis, and targeted knockouts. Phylogenetic and genomic PR:PROJECT_SUMMARY analyses show this operon distributed across abundant bacterial clades, PR:PROJECT_SUMMARY including coastal copiotrophs (e.g., Rhodobacterales) and open-ocean oligotrophs PR:PROJECT_SUMMARY (e.g., SAR11, SAR116). High-resolution mass spectrometry revealed PR:PROJECT_SUMMARY N-methylglutamic acid and glutamic acid as key metabolic products of homarine in PR:PROJECT_SUMMARY both model and natural systems, with N-methylglutamate dehydrogenase catalyzing PR:PROJECT_SUMMARY their conversion. Metatranscriptomics showed responsive and in situ expression PR:PROJECT_SUMMARY of hom genes aligned with homarine availability. These findings uncover the PR:PROJECT_SUMMARY genetic and metabolic basis of homarine degradation, establish its ecological PR:PROJECT_SUMMARY relevance, and highlight homarine as a versatile growth substrate that feeds PR:PROJECT_SUMMARY into central metabolism via glutamic acid in diverse marine bacteria. PR:INSTITUTE University of Washington, School of Oceanography PR:LAST_NAME Heal PR:FIRST_NAME Katherine PR:ADDRESS 1501 NE Boat Street, Marine Science Building, Room G, Seattle PR:EMAIL katherine.heal@pnnl.gov PR:PHONE 612-616-4840 PR:DOI http://dx.doi.org/10.21228/M8R54Z #STUDY ST:STUDY_TITLE Homarine catabolism: Cobetia sp. OBi1 comparative metabolomics under homarine ST:STUDY_TITLE and glucose supported growth ST:STUDY_SUMMARY To uncover genes involved in homarine degradation, we grew Cobetia sp. OBi1 in ST:STUDY_SUMMARY minimal media with homarine as the sole carbon source and conducted comparative ST:STUDY_SUMMARY transcriptomics and metabolomics experiments. We compared three conditions: ST:STUDY_SUMMARY glucose (12 mM C, 0.8 mM NH4, control), homarine (12 mM C, no additional NH4), ST:STUDY_SUMMARY and glucose + homarine (12 mM C, 1 mM C, respectively with 0.8 mM NH4). ST:INSTITUTE University of Washington, School of Oceanography ST:LAST_NAME Heal ST:FIRST_NAME Katherine ST:ADDRESS 1501 NE Boat Street, Marine Science Building, Room G, Seattle ST:EMAIL katherine.heal@pnnl.gov ST:PHONE 612-616-4840 ST:SUBMIT_DATE 2025-10-27 #SUBJECT SU:SUBJECT_TYPE Bacteria SU:SUBJECT_SPECIES Cobetia marina SU:TAXONOMY_ID 28258 SU:GENOTYPE_STRAIN OBi1 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS - 211202_Smp_OBi1_1 Sample source:Cell pellet | Treatment:Glucose RAW_FILE_NAME(Raw file name)=211202_Smp_OBi1_1.raw SUBJECT_SAMPLE_FACTORS - 211202_Smp_OBi1_2 Sample source:Cell pellet | Treatment:Glucose RAW_FILE_NAME(Raw file name)=211202_Smp_OBi1_2.raw SUBJECT_SAMPLE_FACTORS - 211202_Smp_OBi1_3 Sample source:Cell pellet | Treatment:Glucose RAW_FILE_NAME(Raw file name)=211202_Smp_OBi1_3.raw SUBJECT_SAMPLE_FACTORS - 211202_Smp_OBi1_7 Sample source:Cell pellet | Treatment:Glucose + homarine RAW_FILE_NAME(Raw file name)=211202_Smp_OBi1_7.raw SUBJECT_SAMPLE_FACTORS - 211202_Smp_OBi1_8 Sample source:Cell pellet | Treatment:Glucose + homarine RAW_FILE_NAME(Raw file name)=211202_Smp_OBi1_8.raw SUBJECT_SAMPLE_FACTORS - 211202_Smp_OBi1_9 Sample source:Cell pellet | Treatment:Glucose + homarine RAW_FILE_NAME(Raw file name)=211202_Smp_OBi1_9.raw SUBJECT_SAMPLE_FACTORS - 211202_Smp_OBi1_4 Sample source:Cell pellet | Treatment:Homarine RAW_FILE_NAME(Raw file name)=211202_Smp_OBi1_4.raw SUBJECT_SAMPLE_FACTORS - 211202_Smp_OBi1_5 Sample source:Cell pellet | Treatment:Homarine RAW_FILE_NAME(Raw file name)=211202_Smp_OBi1_5.raw SUBJECT_SAMPLE_FACTORS - 211202_Smp_OBi1_6 Sample source:Cell pellet | Treatment:Homarine RAW_FILE_NAME(Raw file name)=211202_Smp_OBi1_6.raw SUBJECT_SAMPLE_FACTORS - 220302_Smp_OBi1_1 Sample source:Supernatant | Treatment:Glucose RAW_FILE_NAME(Raw file name)=220302_Smp_OBi1_1.raw SUBJECT_SAMPLE_FACTORS - 220302_Smp_OBi1_2 Sample source:Supernatant | Treatment:Glucose RAW_FILE_NAME(Raw file name)=220302_Smp_OBi1_2.raw SUBJECT_SAMPLE_FACTORS - 220302_Smp_OBi1_3 Sample source:Supernatant | Treatment:Glucose RAW_FILE_NAME(Raw file name)=220302_Smp_OBi1_3.raw SUBJECT_SAMPLE_FACTORS - 220302_Smp_OBi1_7 Sample source:Supernatant | Treatment:Glucose + homarine RAW_FILE_NAME(Raw file name)=220302_Smp_OBi1_7.raw SUBJECT_SAMPLE_FACTORS - 220302_Smp_OBi1_8 Sample source:Supernatant | Treatment:Glucose + homarine RAW_FILE_NAME(Raw file name)=220302_Smp_OBi1_8.raw SUBJECT_SAMPLE_FACTORS - 220302_Smp_OBi1_9 Sample source:Supernatant | Treatment:Glucose + homarine RAW_FILE_NAME(Raw file name)=220302_Smp_OBi1_9.raw SUBJECT_SAMPLE_FACTORS - 220302_Smp_OBi1_4 Sample source:Supernatant | Treatment:Homarine RAW_FILE_NAME(Raw file name)=220302_Smp_OBi1_4.raw SUBJECT_SAMPLE_FACTORS - 220302_Smp_OBi1_5 Sample source:Supernatant | Treatment:Homarine RAW_FILE_NAME(Raw file name)=220302_Smp_OBi1_5.raw SUBJECT_SAMPLE_FACTORS - 220302_Smp_OBi1_6 Sample source:Supernatant | Treatment:Homarine RAW_FILE_NAME(Raw file name)=220302_Smp_OBi1_6.raw #COLLECTION CO:COLLECTION_SUMMARY Isolate Owen Beach isolate 1 (OBi1) was obtained by enrichment culturing of CO:COLLECTION_SUMMARY seawater collected in Owen Beach (Tacoma, Washington). Seawater was collected in CO:COLLECTION_SUMMARY sterile polycarbonate bottles in February 2020. We amended 100 mL seawater CO:COLLECTION_SUMMARY samples with 12 mM C homarine and 0.1 mM sodium phosphate and incubated in CO:COLLECTION_SUMMARY aerated flasks at 22°C. Culture OD600 was measured daily to monitor growth. CO:COLLECTION_SUMMARY After three days, 50 uL samples were streaked on agar plates made with marine CO:COLLECTION_SUMMARY broth (Difco 2216) to isolate single colonies. Single colonies were picked and CO:COLLECTION_SUMMARY re-streaked twice in the same agar media. The isolated colonies were tested for CO:COLLECTION_SUMMARY growth on homarine in seawater from Owen Beach sterilized by filtering through a CO:COLLECTION_SUMMARY 0.22 um PES membrane and autoclaving. We supplied the medium with homarine and CO:COLLECTION_SUMMARY phosphate as before. OBi1 colonies re-grew on homarine and the isolate was CO:COLLECTION_SUMMARY selected for further analysis. Cobetia sp. OBi1 was grown in three conditions, CO:COLLECTION_SUMMARY as described for comparative transcriptomic analyses: homarine, glucose, and CO:COLLECTION_SUMMARY glucose+homarine. Overnight cultures of Cobetia sp. OBi1 (100 mL) were grown at CO:COLLECTION_SUMMARY 25°C grown in glucose-amended seawater media as described for Cobetia sp. OBi1 CO:COLLECTION_SUMMARY transcriptomics experiments. Next, 10 mL subsamples were taken from overnight CO:COLLECTION_SUMMARY culture and centrifuged for 15 minutes at 2800 g, and resuspended in the CO:COLLECTION_SUMMARY experimental growth media homarine, glucose, or glucose+homarine, in triplicate CO:COLLECTION_SUMMARY (again, as described for Cobetia sp. OBi1 transcriptomics experiments). These CO:COLLECTION_SUMMARY samples were incubated for 1 hr at 25°C in a dark shaker before harvesting. CO:COLLECTION_SUMMARY Cells were harvested by centrifugation for 15 minutes at 2800 g, and supernatant CO:COLLECTION_SUMMARY was collected and filtered through a 0.22 um PES membrane filter. Cell pellets CO:COLLECTION_SUMMARY and supernatant samples were stored at -80°C and -20°C, respectively. CO:SAMPLE_TYPE Bacterial cells #TREATMENT TR:TREATMENT_SUMMARY Cobetia sp. OBi1 was grown in three conditions, as described for comparative TR:TREATMENT_SUMMARY transcriptomic analyses: homarine, glucose, and glucose+homarine. Overnight TR:TREATMENT_SUMMARY cultures of Cobetia sp. OBi1 (100 mL) were grown at 25°C grown in TR:TREATMENT_SUMMARY glucose-amended seawater media as described for Cobetia sp. OBi1 transcriptomics TR:TREATMENT_SUMMARY experiments. #SAMPLEPREP SP:SAMPLEPREP_SUMMARY For particulate metabolomics, cell pellets were extracted using a combination of SP:SAMPLEPREP_SUMMARY mechanical and chemical disruption techniques as described in previous work SP:SAMPLEPREP_SUMMARY (Boysen et al 2018). Metabolites from the supernatant were extracted using a SP:SAMPLEPREP_SUMMARY cation-exchange-based solid phase extraction technique as described previously SP:SAMPLEPREP_SUMMARY (Sacks et al 2022), with 1 mL of supernatant diluted into 10 mL of HPLC grade SP:SAMPLEPREP_SUMMARY water. Isotopically-labeled internal standards were added for normalization SP:SAMPLEPREP_SUMMARY purposes, as reported in Table S17 of SP:SAMPLEPREP_SUMMARY https://doi.org/10.21203/rs.3.rs-7359689/v1 #CHROMATOGRAPHY CH:CHROMATOGRAPHY_SUMMARY For HILIC chromatography, a SeQuant ZIC-pHILIC column (5 um particle size, 2.1 CH:CHROMATOGRAPHY_SUMMARY mm x 150 mm, from Millipore) was used with 10 mM ammonium carbonate in 85:15 CH:CHROMATOGRAPHY_SUMMARY acetonitrile to water (Solvent A) and 10 mM ammonium carbonate in 85:15 water to CH:CHROMATOGRAPHY_SUMMARY acetonitrile (Solvent B) at a flow rate of 0.15 mL/min. This column was compared CH:CHROMATOGRAPHY_SUMMARY with a Waters UPLC BEH amide and a Millipore cHILIC column; the pHILIC showed CH:CHROMATOGRAPHY_SUMMARY superior reproducibility and peak shapes. The column was held at 100% A for 2 CH:CHROMATOGRAPHY_SUMMARY minutes, ramped to 64% B over 18 minutes, ramped to 100% B over 1 minute, held CH:CHROMATOGRAPHY_SUMMARY at 100% B for 5 minutes, and equilibrated at 100% A for 25 minutes (50 minutes CH:CHROMATOGRAPHY_SUMMARY total). The column was maintained at 30 C. The injection volume was 2 µL for CH:CHROMATOGRAPHY_SUMMARY samples and standard mixes. When starting a batch, the column was equilibrated CH:CHROMATOGRAPHY_SUMMARY at the starting conditions for at least 30 minutes. To improve the performance CH:CHROMATOGRAPHY_SUMMARY of the HILIC column, we maintained the same injection volume, kept the CH:CHROMATOGRAPHY_SUMMARY instrument running water blanks between samples as necessary, and injected CH:CHROMATOGRAPHY_SUMMARY standards in a representative matrix in addition to standards in water. After CH:CHROMATOGRAPHY_SUMMARY each batch, the column was flushed with 10 mM ammonium carbonate in 85:15 water CH:CHROMATOGRAPHY_SUMMARY to acetonitrile for 20 to 30 minutes. CH:METHODS_ID CH001683 CH:INSTRUMENT_NAME Waters Acquity I-Class CH:COLUMN_NAME SeQuant ZIC- pHILIC (150 x 2.1mm,5um) CH:COLUMN_TEMPERATURE 30 CH:FLOW_GRADIENT The column was held at 100% A for 2 minutes, ramped to 64% B over 18 minutes, CH:FLOW_GRADIENT ramped to 100% B over 1 minute, held at 100% B for 5 minutes, and equilibrated CH:FLOW_GRADIENT at 100% A for 25 minutes CH:FLOW_RATE 0.15 mL/min CH:SOLVENT_A 85% acetonitrile/15% water; 10 mM ammonium carbonate CH:SOLVENT_B 15% acetonitrile/85% water; 10 mM ammonium carbonate CH:CHROMATOGRAPHY_TYPE HILIC #ANALYSIS AN:ANALYSIS_TYPE MS #MS MS:INSTRUMENT_NAME Thermo Q Exactive HF hybrid Orbitrap MS:INSTRUMENT_TYPE Orbitrap MS:MS_TYPE ESI MS:MS_COMMENTS MS acquisition Comments: Polarity switching was used with a scan range of 60 to MS:MS_COMMENTS 900 m/z and a resolution of 60,000. MS parameters were as follows: capillary MS:MS_COMMENTS temperature was 320 ∞C, the H-ESI spray voltage was 3.3 kV, and the auxiliary MS:MS_COMMENTS gas heater temperature was 100 ∞C. The S-lens RF level was 65. Sheath gas, MS:MS_COMMENTS auxiliary gas, and sweep gas flow rates were maintained at 16, 3, and 1, MS:MS_COMMENTS respectively. MS:ION_MODE NEGATIVE #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS normalized peak area MS_METABOLITE_DATA_START Samples 211202_Smp_OBi1_1 211202_Smp_OBi1_2 211202_Smp_OBi1_3 211202_Smp_OBi1_7 211202_Smp_OBi1_8 211202_Smp_OBi1_9 211202_Smp_OBi1_4 211202_Smp_OBi1_5 211202_Smp_OBi1_6 220302_Smp_OBi1_1 220302_Smp_OBi1_2 220302_Smp_OBi1_3 220302_Smp_OBi1_7 220302_Smp_OBi1_8 220302_Smp_OBi1_9 220302_Smp_OBi1_4 220302_Smp_OBi1_5 220302_Smp_OBi1_6 Factors Sample source:Cell pellet | Treatment:Glucose Sample source:Cell pellet | Treatment:Glucose Sample source:Cell pellet | Treatment:Glucose Sample source:Cell pellet | Treatment:Glucose + homarine Sample source:Cell pellet | Treatment:Glucose + homarine Sample source:Cell pellet | Treatment:Glucose + homarine Sample source:Cell pellet | Treatment:Homarine Sample source:Cell pellet | Treatment:Homarine Sample source:Cell pellet | Treatment:Homarine Sample source:Supernatant | Treatment:Glucose Sample source:Supernatant | Treatment:Glucose Sample source:Supernatant | Treatment:Glucose Sample source:Supernatant | Treatment:Glucose + homarine Sample source:Supernatant | Treatment:Glucose + homarine Sample source:Supernatant | Treatment:Glucose + homarine Sample source:Supernatant | Treatment:Homarine Sample source:Supernatant | Treatment:Homarine Sample source:Supernatant | Treatment:Homarine 2-Hydroxy-4-(methylthio)butyric acid 179593.0000 116270.0000 133076.0000 141116.0000 128101.0000 148369.0000 225334.0000 143885.0000 207379.0000 2-Ketoglutaric acid 35703288.0000 51563072.0000 38981516.0000 42568576.0000 41247588.0000 31743020.0000 17446634.0000 14462896.0000 32865838.0000 3-Phosphoglycerate 2710229.1641 2067609.7520 2649468.2840 2374147.9186 1625775.7495 1438631.6176 275433.3821 308408.1763 251578.6796 5-Oxoproline 72770846.1161 51890032.8614 59251699.4117 55597027.0944 65249150.2541 55880994.3021 21155067.6134 19081396.1465 18639030.5888 Adenosine diphosphate 1264633.0000 3328658.0000 1228335.0000 1010396.0000 2929693.0000 5066863.0000 2770472.0000 3223078.0000 Adenosine monophosphate 1593471.0000 6425140.0000 2018471.0000 1392695.0000 9480275.0000 15553344.0000 5433902.0000 7743475.0000 Adenosine triphosphate 100905.6334 204788.9073 61438.1908 68543.8337 180211.0641 146865.1039 268197.5092 cis-Aconitic acid 2096345.5924 1863759.5914 1503772.8182 1282761.6879 1264152.9015 1183878.0730 714060.6816 901919.5984 718333.8381 Citric acid 58065585.8014 55926703.2471 63742740.2577 54789862.5230 52573912.4840 38256817.0182 83859900.5434 105678421.9092 45287903.5355 D-Fructose 6-phosphate 13525130.8934 11222025.6458 13025018.1997 10790043.6192 11003161.3426 7500690.5871 12914688.5348 8144497.4624 6884866.3036 D-Gluconic acid 178625141.3752 295796380.1177 305776742.8464 764290386.8699 844031060.4306 886127247.8364 4873913.8009 4640537.7051 5195306.0240 D-Glucose 6-phosphate 26234409.1846 18586621.3382 19282914.3635 17196039.5008 19374725.9576 13751561.7374 6804574.6561 5561496.9954 3822253.7826 Dihydroxyacetone phosphate 2718597.0000 3202471.0000 3057831.0000 2073475.0000 2122898.0000 1780497.0000 294955.0000 199185.0000 135434.0000 D-Ribose 5-phosphate 3137248.0000 4474872.0000 4405642.0000 3261666.0000 3142808.0000 2997147.0000 2515558.0000 754760.0000 2019297.0000 Fumaric acid 4689516.6830 6445240.1952 5432720.3933 22155052.4272 25964450.3399 17295269.9148 16344104.7538 11653929.0403 13441296.5126 Glycerophosphoric acid 16283273.0000 17552712.0000 26651968.0000 14164889.0000 15651891.0000 12849753.0000 10253338.0000 4913386.0000 6109368.0000 Guanosine monophosphate 326031.0000 1191745.0000 342701.0000 203139.0000 1944896.0000 4618819.0000 799695.0000 523197.0000 Inosine 11753295.4710 5846059.9744 8153535.5154 7732616.1320 5374313.5227 4197296.8818 147380.1158 270425.8599 L-Cysteic acid 246518.0000 302501.0000 269134.0000 130698.0000 59195.0000 48736.0000 168427.0000 85731.0000 61736.0000 Malic acid 80193672.0000 89435784.0000 88128864.0000 80488904.0000 92688744.0000 77595408.0000 44320824.0000 26884548.0000 55143312.0000 N-Acetyl-L-glutamic acid 129755760.0000 130319744.0000 126733760.0000 103714304.0000 110088304.0000 100278992.0000 3628926.0000 5948816.0000 4067639.0000 NAD 8081089.8766 7844841.3600 6209987.2880 4960045.8468 8238369.6534 5026421.0329 46840.7958 6118540.8033 3840738.3665 NADH 1093043.0000 913786.0000 752084.0000 NADP 68832.0000 51193.0000 138436.0000 46243.0000 94232.0000 121077.0000 60002.0000 NADPH 573042.7089 492021.4739 347336.3759 260934.6706 533024.0241 61279.3869 47463.2285 Orotic acid 8894019.0000 13856842.0000 11470589.0000 9383700.0000 8296196.0000 5919054.0000 105005.0000 86188.0000 43596.0000 Phosphoenolpyruvic acid 1510745.8812 1621482.8243 1927754.2953 1382086.3126 1268371.0670 829348.8261 703553.9887 400983.3334 633025.4306 Propionylglycine 4632796.4093 3898907.8230 3830661.1205 3812323.2819 3899275.7418 3127747.4339 2326716.9554 2413076.2516 2223649.8757 Succinic acid 142391600.0000 108036552.0000 153378688.0000 129822352.0000 141560800.0000 143403792.0000 58541936.0000 52394984.0000 36363508.0000 Taurine Thymidine 9925175.0000 7130846.0000 10436034.0000 8485321.0000 6016284.0000 5351046.0000 5487190.0000 3243111.0000 2514666.0000 Thymine 58862600.0000 41261012.0000 57655368.0000 49601312.0000 37835228.0000 32849014.0000 32707688.0000 18608176.0000 16032521.0000 UDP-glucose 7490825.1645 6561890.0674 4803537.1152 3840299.2674 4751292.4289 2502656.9999 570110.5662 2431168.0813 1820897.7346 UDP-N-acetylglucosamine 5093319.7405 4930128.4432 4844995.4443 4772280.2015 4924073.6887 4183803.0024 415501.8861 2553070.8890 1643769.8481 Uracil 12208926.0000 33677744.0000 46669584.0000 18201190.0000 22918740.0000 15553607.0000 24470088.0000 4673360.0000 32344576.0000 Uridine 203954453.9065 105847740.3841 198215196.9283 150455269.2724 84583326.7765 57037060.7023 81643587.7200 33846403.3081 17676615.3892 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name pubchem_id inchi_key kegg_id other_id other_id_type ri ri_type moverz_quant 2-Hydroxy-4-(methylthio)butyric acid NA 149.027242 2-Ketoglutaric acid cpd:C00026 145.0137 3-Phosphoglycerate cpd:C00597 184.985118 5-Oxoproline cpd:C01879 128.034768 Adenosine diphosphate cpd:C00008 426.021596 Adenosine monophosphate cpd:C00020 346.055262 Adenosine triphosphate cpd:C00002 505.987929 cis-Aconitic acid cpd:C00417 173.008615 Citric acid cpd:C00158 191.01918 D-Fructose 6-phosphate cpd:C00085 259.021897 D-Gluconic acid cpd:C00257 195.05048 D-Glucose 6-phosphate cpd:C00092 259.021897 Dihydroxyacetone phosphate cpd:C00111 168.990203 D-Ribose 5-phosphate cpd:C00117 229.011333 Fumaric acid cpd:C00122 115.003135 Glycerophosphoric acid cpd:C00093 171.005853 Guanosine monophosphate cpd:C00144 362.050178 Inosine cpd:C00294 267.072946 L-Cysteic acid cpd:C00506 167.996671 Malic acid cpd:C00149 133.0137 N-Acetyl-L-glutamic acid cpd:C00624 188.055899 NAD cpd:C00003 662.101304 NADH cpd:C00004 664.116954 NADP cpd:C00006 742.067637 NADPH cpd:C00005 744.083287 Orotic acid cpd:C00295 155.009283 Phosphoenolpyruvic acid cpd:C00074 166.974553 Propionylglycine NA 130.050419 Succinic acid cpd:C00042 117.018785 Taurine cpd:C00245 124.006841 Thymidine cpd:C00214 241.082448 Thymine cpd:C00178 125.035103 UDP-glucose cpd:C00029 565.047204 UDP-N-acetylglucosamine cpd:C00043 606.073753 Uracil cpd:C00106 111.019453 Uridine cpd:C00299 243.061713 METABOLITES_END #END