#METABOLOMICS WORKBENCH FienVerdoodt_20251111_070501 DATATRACK_ID:6655 STUDY_ID:ST004369 ANALYSIS_ID:AN007298 PROJECT_ID:PR002768
VERSION             	1
CREATED_ON             	November 19, 2025, 5:26 pm
#PROJECT
PR:PROJECT_TITLE                 	Medium chain triglycerides induce metabolic shifts in dogs with idiopathic
PR:PROJECT_TITLE                 	epilepsy: a multi-omics approach
PR:PROJECT_TYPE                  	Double blinded placebo-controlled dietary trial in dogs with idiopathic epilepsy
PR:PROJECT_SUMMARY               	Background: Medium chain triglycerides (MCT) are a class of dietary lipids with
PR:PROJECT_SUMMARY               	proposed beneficial health effects in animals and humans alike. Their mechanism
PR:PROJECT_SUMMARY               	of action in specific applications, like epilepsy, remains however unclear.
PR:PROJECT_SUMMARY               	Therefore, the aim of this study was to identify alterations in the fecal and
PR:PROJECT_SUMMARY               	plasma metabolome and fecal microbiome in dogs with idiopathic epilepsy (IE)
PR:PROJECT_SUMMARY               	following an MCT diet. A double-blinded placebo controlled dietary trial
PR:PROJECT_SUMMARY               	including 32 dogs with drug-resistant IE was established. Dogs were randomly
PR:PROJECT_SUMMARY               	allocated to an MCT (n = 16) or placebo diet (n = 16) for 3 months. Feces and
PR:PROJECT_SUMMARY               	plasma were collected at the start and end of the dietary trial. The metabolome
PR:PROJECT_SUMMARY               	of plasma and feces were analysed using liquid chromatography coupled to high
PR:PROJECT_SUMMARY               	resolution mass spectrometry, and the fecal bacterial phylogeny was examined
PR:PROJECT_SUMMARY               	using 16S rRNA sequencing. Fecal metabolome, plasma metabolome and fecal
PR:PROJECT_SUMMARY               	microbiome data were integrated using the DIABLO framework. Results: The MCT
PR:PROJECT_SUMMARY               	diet elicited distinct metabolic adaptations and modulated gut microbial
PR:PROJECT_SUMMARY               	composition, notably characterized by alterations in histidine and energy
PR:PROJECT_SUMMARY               	metabolism, a reduction in Escherichia-Shigella, and an enrichment of genera
PR:PROJECT_SUMMARY               	including Ruminococcus and Faecalitalea. Multiple correlations were observed
PR:PROJECT_SUMMARY               	between significantly altered microbial taxa and fecal metabolites like e.g.
PR:PROJECT_SUMMARY               	1-methylhistidine and creatine. Conclusion: Our findings demonstrate that a diet
PR:PROJECT_SUMMARY               	rich in MCT does not only support peripheral energy metabolism, evidenced by
PR:PROJECT_SUMMARY               	stable carnitine metabolites and reduced plasma threonine, but also reshapes
PR:PROJECT_SUMMARY               	microbial composition. Therefore, potential involvement of the
PR:PROJECT_SUMMARY               	microbiota–gut–brain axis in the mechanism of action for MCT in dogs with IE
PR:PROJECT_SUMMARY               	was underscored.
PR:INSTITUTE                     	Ghent University
PR:DEPARTMENT                    	DI11 - DI04
PR:LABORATORY                    	ECAN - LIMET
PR:LAST_NAME                     	Verdoodt
PR:FIRST_NAME                    	Fien
PR:ADDRESS                       	Salisburylaan 133, Merelbeke, Flanders, 9820, Belgium
PR:EMAIL                         	fien.verdoodt@ugent.be
PR:PHONE                         	+32476076770
PR:FUNDING_SOURCE                	FWO
PR:PUBLICATIONS                  	Preprint available at https://doi.org/10.21203/rs.3.rs-8078706/v1
#STUDY
ST:STUDY_TITLE                   	Medium chain triglycerides induce metabolic shifts in dogs with idiopathic
ST:STUDY_TITLE                   	epilepsy: a multi-omics approach - plasma metabolome
ST:STUDY_TYPE                    	Double-blinded placebo controlled dietary trial in dogs - Plasma metabolome
ST:STUDY_SUMMARY                 	Plasma metabolome dataset - Background: Medium chain triglycerides (MCT) are a
ST:STUDY_SUMMARY                 	class of dietary lipids with proposed beneficial health effects in animals and
ST:STUDY_SUMMARY                 	humans alike. Their mechanism of action in specific applications, like epilepsy,
ST:STUDY_SUMMARY                 	remains however unclear. Therefore, the aim of this study was to identify
ST:STUDY_SUMMARY                 	alterations in the fecal and plasma metabolome and fecal microbiome in dogs with
ST:STUDY_SUMMARY                 	idiopathic epilepsy (IE) following an MCT diet. A double-blinded placebo
ST:STUDY_SUMMARY                 	controlled dietary trial including 32 dogs with drug-resistant IE was
ST:STUDY_SUMMARY                 	established. Dogs were randomly allocated to an MCT (n = 16) or placebo diet (n
ST:STUDY_SUMMARY                 	= 16) for 3 months. Feces and plasma were collected at the start and end of the
ST:STUDY_SUMMARY                 	dietary trial. The metabolome of plasma and feces were analysed using liquid
ST:STUDY_SUMMARY                 	chromatography coupled to high resolution mass spectrometry, and the fecal
ST:STUDY_SUMMARY                 	bacterial phylogeny was examined using 16S rRNA sequencing. Fecal metabolome,
ST:STUDY_SUMMARY                 	plasma metabolome and fecal microbiome data were integrated using the DIABLO
ST:STUDY_SUMMARY                 	framework. Results: The MCT diet elicited distinct metabolic adaptations and
ST:STUDY_SUMMARY                 	modulated gut microbial composition, notably characterized by alterations in
ST:STUDY_SUMMARY                 	histidine and energy metabolism, a reduction in Escherichia-Shigella, and an
ST:STUDY_SUMMARY                 	enrichment of genera including Ruminococcus and Faecalitalea. Multiple
ST:STUDY_SUMMARY                 	correlations were observed between significantly altered microbial taxa and
ST:STUDY_SUMMARY                 	fecal metabolites like e.g. 1-methylhistidine and creatine. Conclusion: Our
ST:STUDY_SUMMARY                 	findings demonstrate that a diet rich in MCT does not only support peripheral
ST:STUDY_SUMMARY                 	energy metabolism, evidenced by stable carnitine metabolites and reduced plasma
ST:STUDY_SUMMARY                 	threonine, but also reshapes microbial composition. Therefore, potential
ST:STUDY_SUMMARY                 	involvement of the microbiota–gut–brain axis in the mechanism of action for
ST:STUDY_SUMMARY                 	MCT in dogs with IE was underscored.
ST:INSTITUTE                     	Ghent University
ST:DEPARTMENT                    	DI11 - DI04
ST:LABORATORY                    	ECAN - LIMET
ST:LAST_NAME                     	Verdoodt
ST:FIRST_NAME                    	Fien
ST:ADDRESS                       	Salisburylaan 133
ST:EMAIL                         	fien.verdoodt@ugent.be
ST:PHONE                         	+32476076770
ST:NUM_GROUPS                    	2
ST:TOTAL_SUBJECTS                	32
ST:NUM_MALES                     	20
ST:NUM_FEMALES                   	12
ST:STUDY_COMMENTS                	MCT vs placebo
#SUBJECT
SU:SUBJECT_TYPE                  	Mammal
SU:SUBJECT_SPECIES               	Canis lupus familiaris
SU:TAXONOMY_ID                   	9615
SU:AGE_OR_AGE_RANGE              	1-10 years old
SU:WEIGHT_OR_WEIGHT_RANGE        	5.1-52.4 kg
SU:GENDER                        	Male and female
SU:ANIMAL_ANIMAL_SUPPLIER        	pets
SU:ANIMAL_HOUSING                	at home (pets)
SU:ANIMAL_FEED                   	MCT-diet or isocaloric placebo diet
SU:ANIMAL_INCLUSION_CRITERIA     	idiopathic epilepsy Tier I (IVETF)
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data
SUBJECT_SAMPLE_FACTORS           	1	Dog1T3	Sample source:plasma | Diet:MCT | Timepoint:3	RAW_FILE_NAME(RAW file name2)=240924_sf023.raw; RAW_FILE_NAME(mzXML file name)=240924_sf023.mzXML; Sex=Fe; Breed=CV; Age=10; Weight=16.6; AgeGroup=geriatric; BCS=5
SUBJECT_SAMPLE_FACTORS           	1	Dog1T0	Sample source:plasma | Diet:MCT | Timepoint:0	RAW_FILE_NAME(RAW file name2)=240924_sf054.raw; RAW_FILE_NAME(mzXML file name)=240924_sf054.mzXML; Sex=Fe; Breed=CV; Age=10; Weight=16.6; AgeGroup=geriatric; BCS=5
SUBJECT_SAMPLE_FACTORS           	3	Dog3T0	Sample source:plasma | Diet:Pl | Timepoint:0	RAW_FILE_NAME(RAW file name2)=240924_sf067.raw; RAW_FILE_NAME(mzXML file name)=240924_sf067.mzXML; Sex=Ma; Breed=Franse bulldog; Age=2; Weight=16.5; AgeGroup=adult; BCS=5
SUBJECT_SAMPLE_FACTORS           	3	Dog3T3	Sample source:plasma | Diet:Pl | Timepoint:3	RAW_FILE_NAME(RAW file name2)=240924_sf094.raw; RAW_FILE_NAME(mzXML file name)=240924_sf094.mzXML; Sex=Ma; Breed=Franse bulldog; Age=2; Weight=16.5; AgeGroup=adult; BCS=5
SUBJECT_SAMPLE_FACTORS           	4	Dog4T0	Sample source:plasma | Diet:Pl | Timepoint:0	RAW_FILE_NAME(RAW file name2)=240924_sf043.raw; RAW_FILE_NAME(mzXML file name)=240924_sf043.mzXML; Sex=Fe; Breed=American Cocker Spaniel; Age=5; Weight=16.5; AgeGroup=adult; BCS=6
SUBJECT_SAMPLE_FACTORS           	4	Dog4T3	Sample source:plasma | Diet:Pl | Timepoint:3	RAW_FILE_NAME(RAW file name2)=240924_sf048.raw; RAW_FILE_NAME(mzXML file name)=240924_sf048.mzXML; Sex=Fe; Breed=American Cocker Spaniel; Age=5; Weight=16.5; AgeGroup=adult; BCS=6
SUBJECT_SAMPLE_FACTORS           	5	Dog5T0	Sample source:plasma | Diet:Pl | Timepoint:0	RAW_FILE_NAME(RAW file name2)=240924_sf077.raw; RAW_FILE_NAME(mzXML file name)=240924_sf077.mzXML; Sex=Ma; Breed=Cane corso italiano; Age=3; Weight=48.4; AgeGroup=adult; BCS=5
SUBJECT_SAMPLE_FACTORS           	5	Dog5T3	Sample source:plasma | Diet:Pl | Timepoint:3	RAW_FILE_NAME(RAW file name2)=240924_sf140.raw; RAW_FILE_NAME(mzXML file name)=240924_sf140.mzXML; Sex=Ma; Breed=Cane corso italiano; Age=3; Weight=48.4; AgeGroup=adult; BCS=5
SUBJECT_SAMPLE_FACTORS           	6	Dog6T0	Sample source:plasma | Diet:Pl | Timepoint:0	RAW_FILE_NAME(RAW file name2)=240924_sf119.raw; RAW_FILE_NAME(mzXML file name)=240924_sf119.mzXML; Sex=Ma; Breed=Australische herdershond; Age=7; Weight=24.8; AgeGroup=senior; BCS=5
SUBJECT_SAMPLE_FACTORS           	6	Dog6T3	Sample source:plasma | Diet:Pl | Timepoint:3	RAW_FILE_NAME(RAW file name2)=240924_sf160.raw; RAW_FILE_NAME(mzXML file name)=240924_sf160.mzXML; Sex=Ma; Breed=Australische herdershond; Age=7; Weight=24.8; AgeGroup=senior; BCS=5
SUBJECT_SAMPLE_FACTORS           	7	Dog7T0	Sample source:plasma | Diet:MCT | Timepoint:0	RAW_FILE_NAME(RAW file name2)=240924_sf035.raw; RAW_FILE_NAME(mzXML file name)=240924_sf035.mzXML; Sex=Ms; Breed=Engelse bulldog; Age=5; Weight=23.2; AgeGroup=adult; BCS=5
SUBJECT_SAMPLE_FACTORS           	7	Dog7T3	Sample source:plasma | Diet:MCT | Timepoint:3	RAW_FILE_NAME(RAW file name2)=240924_sf161.raw; RAW_FILE_NAME(mzXML file name)=240924_sf161.mzXML; Sex=Ms; Breed=Engelse bulldog; Age=5; Weight=23.2; AgeGroup=adult; BCS=5
SUBJECT_SAMPLE_FACTORS           	8	Dog8T3	Sample source:plasma | Diet:MCT | Timepoint:3	RAW_FILE_NAME(RAW file name2)=240924_sf056.raw; RAW_FILE_NAME(mzXML file name)=240924_sf056.mzXML; Sex=Ma; Breed=Shih Tzu; Age=7; Weight=8.5; AgeGroup=adult; BCS=5
SUBJECT_SAMPLE_FACTORS           	8	Dog8T0	Sample source:plasma | Diet:MCT | Timepoint:0	RAW_FILE_NAME(RAW file name2)=240924_sf120.raw; RAW_FILE_NAME(mzXML file name)=240924_sf120.mzXML; Sex=Ma; Breed=Shih Tzu; Age=7; Weight=8.5; AgeGroup=adult; BCS=5
SUBJECT_SAMPLE_FACTORS           	9	Dog9T3	Sample source:plasma | Diet:Pl | Timepoint:3	RAW_FILE_NAME(RAW file name2)=240924_sf041.raw; RAW_FILE_NAME(mzXML file name)=240924_sf041.mzXML; Sex=Ms; Breed=CV; Age=1; Weight=38.6; AgeGroup=adult; BCS=6
SUBJECT_SAMPLE_FACTORS           	9	Dog9T0	Sample source:plasma | Diet:Pl | Timepoint:0	RAW_FILE_NAME(RAW file name2)=240924_sf125.raw; RAW_FILE_NAME(mzXML file name)=240924_sf125.mzXML; Sex=Ms; Breed=CV; Age=1; Weight=38.6; AgeGroup=adult; BCS=6
SUBJECT_SAMPLE_FACTORS           	10	Dog10T0	Sample source:plasma | Diet:MCT | Timepoint:0	RAW_FILE_NAME(RAW file name2)=240924_sf064.raw; RAW_FILE_NAME(mzXML file name)=240924_sf064.mzXML; Sex=Ma; Breed=Dalmatiër; Age=2; Weight=33.6; AgeGroup=adult; BCS=6
SUBJECT_SAMPLE_FACTORS           	10	Dog10T3	Sample source:plasma | Diet:MCT | Timepoint:3	RAW_FILE_NAME(RAW file name2)=240924_sf133.raw; RAW_FILE_NAME(mzXML file name)=240924_sf133.mzXML; Sex=Ma; Breed=Dalmatiër; Age=2; Weight=33.6; AgeGroup=adult; BCS=6
SUBJECT_SAMPLE_FACTORS           	11	Dog11T0	Sample source:plasma | Diet:MCT | Timepoint:0	RAW_FILE_NAME(RAW file name2)=240924_sf052.raw; RAW_FILE_NAME(mzXML file name)=240924_sf052.mzXML; Sex=Ma; Breed=Rottweiler; Age=3; Weight=52.4; AgeGroup=adult; BCS=5
SUBJECT_SAMPLE_FACTORS           	11	Dog11T3	Sample source:plasma | Diet:MCT | Timepoint:3	RAW_FILE_NAME(RAW file name2)=240924_sf146.raw; RAW_FILE_NAME(mzXML file name)=240924_sf146.mzXML; Sex=Ma; Breed=Rottweiler; Age=3; Weight=52.4; AgeGroup=adult; BCS=5
SUBJECT_SAMPLE_FACTORS           	12	Dog12T0	Sample source:plasma | Diet:MCT | Timepoint:0	RAW_FILE_NAME(RAW file name2)=240924_sf065.raw; RAW_FILE_NAME(mzXML file name)=240924_sf065.mzXML; Sex=Fs; Breed=Friese stabij; Age=7; Weight=16.5; AgeGroup=senior; BCS=4
SUBJECT_SAMPLE_FACTORS           	12	Dog12T3	Sample source:plasma | Diet:MCT | Timepoint:3	RAW_FILE_NAME(RAW file name2)=240924_sf123.raw; RAW_FILE_NAME(mzXML file name)=240924_sf123.mzXML; Sex=Fs; Breed=Friese stabij; Age=7; Weight=16.5; AgeGroup=senior; BCS=4
SUBJECT_SAMPLE_FACTORS           	13	Dog13T0	Sample source:plasma | Diet:Pl | Timepoint:0	RAW_FILE_NAME(RAW file name2)=240924_sf081.raw; RAW_FILE_NAME(mzXML file name)=240924_sf081.mzXML; Sex=Ma; Breed=Franse bulldog; Age=8; Weight=13; AgeGroup=senior; BCS=5
SUBJECT_SAMPLE_FACTORS           	13	Dog13T3	Sample source:plasma | Diet:Pl | Timepoint:3	RAW_FILE_NAME(RAW file name2)=240924_sf141.raw; RAW_FILE_NAME(mzXML file name)=240924_sf141.mzXML; Sex=Ma; Breed=Franse bulldog; Age=8; Weight=13; AgeGroup=senior; BCS=5
SUBJECT_SAMPLE_FACTORS           	16	Dog16T3	Sample source:plasma | Diet:MCT | Timepoint:3	RAW_FILE_NAME(RAW file name2)=240924_sf084.raw; RAW_FILE_NAME(mzXML file name)=240924_sf084.mzXML; Sex=Fs; Breed=Jack Russel terrier; Age=7; Weight=7.5; AgeGroup=adult; BCS=6
SUBJECT_SAMPLE_FACTORS           	16	Dog16T0	Sample source:plasma | Diet:MCT | Timepoint:0	RAW_FILE_NAME(RAW file name2)=240924_sf151.raw; RAW_FILE_NAME(mzXML file name)=240924_sf151.mzXML; Sex=Fs; Breed=Jack Russel terrier; Age=7; Weight=7.5; AgeGroup=adult; BCS=6
SUBJECT_SAMPLE_FACTORS           	17	Dog17T3	Sample source:plasma | Diet:Pl | Timepoint:3	RAW_FILE_NAME(RAW file name2)=240924_sf028.raw; RAW_FILE_NAME(mzXML file name)=240924_sf028.mzXML; Sex=Ma; Breed=Cane Corso Italiano; Age=3; Weight=49; AgeGroup=adult; BCS=3
SUBJECT_SAMPLE_FACTORS           	17	Dog17T0	Sample source:plasma | Diet:Pl | Timepoint:0	RAW_FILE_NAME(RAW file name2)=240924_sf148.raw; RAW_FILE_NAME(mzXML file name)=240924_sf148.mzXML; Sex=Ma; Breed=Cane Corso Italiano; Age=3; Weight=49; AgeGroup=adult; BCS=3
SUBJECT_SAMPLE_FACTORS           	18	Dog18T3	Sample source:plasma | Diet:MCT | Timepoint:3	RAW_FILE_NAME(RAW file name2)=240924_sf055.raw; RAW_FILE_NAME(mzXML file name)=240924_sf055.mzXML; Sex=Fe; Breed=Engelse Cocker spaniel; Age=2; Weight=17.3; AgeGroup=adult; BCS=7
SUBJECT_SAMPLE_FACTORS           	18	Dog18T0	Sample source:plasma | Diet:MCT | Timepoint:0	RAW_FILE_NAME(RAW file name2)=240924_sf083.raw; RAW_FILE_NAME(mzXML file name)=240924_sf083.mzXML; Sex=Fe; Breed=Engelse Cocker spaniel; Age=2; Weight=17.3; AgeGroup=adult; BCS=7
SUBJECT_SAMPLE_FACTORS           	19	Dog19T0	Sample source:plasma | Diet:MCT | Timepoint:0	RAW_FILE_NAME(RAW file name2)=240924_sf106.raw; RAW_FILE_NAME(mzXML file name)=240924_sf106.mzXML; Sex=Ma; Breed=Golden retriever; Age=8; Weight=38.3; AgeGroup=geriatric; BCS=7
SUBJECT_SAMPLE_FACTORS           	19	Dog19T3	Sample source:plasma | Diet:MCT | Timepoint:3	RAW_FILE_NAME(RAW file name2)=240924_sf124.raw; RAW_FILE_NAME(mzXML file name)=240924_sf124.mzXML; Sex=Ma; Breed=Golden retriever; Age=8; Weight=38.3; AgeGroup=geriatric; BCS=7
SUBJECT_SAMPLE_FACTORS           	20	Dog20T0	Sample source:plasma | Diet:MCT | Timepoint:0	RAW_FILE_NAME(RAW file name2)=240924_sf069.raw; RAW_FILE_NAME(mzXML file name)=240924_sf069.mzXML; Sex=Ma; Breed=Border Collie; Age=8; Weight=28; AgeGroup=geriatric; BCS=6
SUBJECT_SAMPLE_FACTORS           	20	Dog20T3	Sample source:plasma | Diet:MCT | Timepoint:3	RAW_FILE_NAME(RAW file name2)=240924_sf162.raw; RAW_FILE_NAME(mzXML file name)=240924_sf162.mzXML; Sex=Ma; Breed=Border Collie; Age=8; Weight=28; AgeGroup=geriatric; BCS=6
SUBJECT_SAMPLE_FACTORS           	21	Dog21T3	Sample source:plasma | Diet:Pl | Timepoint:3	RAW_FILE_NAME(RAW file name2)=240924_sf038.raw; RAW_FILE_NAME(mzXML file name)=240924_sf038.mzXML; Sex=Fs; Breed=Duitse staander; Age=4; Weight=32; AgeGroup=adult; BCS=5
SUBJECT_SAMPLE_FACTORS           	21	Dog21T0	Sample source:plasma | Diet:Pl | Timepoint:0	RAW_FILE_NAME(RAW file name2)=240924_sf112.raw; RAW_FILE_NAME(mzXML file name)=240924_sf112.mzXML; Sex=Fs; Breed=Duitse staander; Age=4; Weight=32; AgeGroup=adult; BCS=5
SUBJECT_SAMPLE_FACTORS           	24	Dog24T3	Sample source:plasma | Diet:Pl | Timepoint:3	RAW_FILE_NAME(RAW file name2)=240924_sf070.raw; RAW_FILE_NAME(mzXML file name)=240924_sf070.mzXML; Sex=Fs; Breed=Sint Bernard; Age=3; Weight=60.8; AgeGroup=adult; BCS=6
SUBJECT_SAMPLE_FACTORS           	24	Dog24T0	Sample source:plasma | Diet:Pl | Timepoint:0	RAW_FILE_NAME(RAW file name2)=240924_sf079.raw; RAW_FILE_NAME(mzXML file name)=240924_sf079.mzXML; Sex=Fs; Breed=Sint Bernard; Age=3; Weight=60.8; AgeGroup=adult; BCS=6
SUBJECT_SAMPLE_FACTORS           	25	Dog25T0	Sample source:plasma | Diet:MCT | Timepoint:0	RAW_FILE_NAME(RAW file name2)=240924_sf062.raw; RAW_FILE_NAME(mzXML file name)=240924_sf062.mzXML; Sex=Fs; Breed=Border Collie; Age=1; Weight=21.55; AgeGroup=adult; BCS=4
SUBJECT_SAMPLE_FACTORS           	25	Dog25T3	Sample source:plasma | Diet:MCT | Timepoint:3	RAW_FILE_NAME(RAW file name2)=240924_sf132.raw; RAW_FILE_NAME(mzXML file name)=240924_sf132.mzXML; Sex=Fs; Breed=Border Collie; Age=1; Weight=21.55; AgeGroup=adult; BCS=4
SUBJECT_SAMPLE_FACTORS           	26	Dog26T0	Sample source:plasma | Diet:Pl | Timepoint:0	RAW_FILE_NAME(RAW file name2)=240924_sf021.raw; RAW_FILE_NAME(mzXML file name)=240924_sf021.mzXML; Sex=Ms; Breed=Border Collie; Age=5; Weight=23; AgeGroup=adult; BCS=6
SUBJECT_SAMPLE_FACTORS           	26	Dog26T3	Sample source:plasma | Diet:Pl | Timepoint:3	RAW_FILE_NAME(RAW file name2)=240924_sf092.raw; RAW_FILE_NAME(mzXML file name)=240924_sf092.mzXML; Sex=Ms; Breed=Border Collie; Age=5; Weight=23; AgeGroup=adult; BCS=6
SUBJECT_SAMPLE_FACTORS           	27	Dog27T3	Sample source:plasma | Diet:MCT | Timepoint:3	RAW_FILE_NAME(RAW file name2)=240924_sf071.raw; RAW_FILE_NAME(mzXML file name)=240924_sf071.mzXML; Sex=Ma; Breed=Zwitserse Witte herder; Age=1; Weight=38.5; AgeGroup=adult; BCS=6
SUBJECT_SAMPLE_FACTORS           	27	Dog27T0	Sample source:plasma | Diet:MCT | Timepoint:0	RAW_FILE_NAME(RAW file name2)=240924_sf122.raw; RAW_FILE_NAME(mzXML file name)=240924_sf122.mzXML; Sex=Ma; Breed=Zwitserse Witte herder; Age=1; Weight=38.5; AgeGroup=adult; BCS=6
SUBJECT_SAMPLE_FACTORS           	28	Dog28T3	Sample source:plasma | Diet:Pl | Timepoint:3	RAW_FILE_NAME(RAW file name2)=240924_sf066.raw; RAW_FILE_NAME(mzXML file name)=240924_sf066.mzXML; Sex=Fs; Breed=Australische Herdershond; Age=6; Weight=28.3; AgeGroup=senior; BCS=7
SUBJECT_SAMPLE_FACTORS           	28	Dog28T0	Sample source:plasma | Diet:Pl | Timepoint:0	RAW_FILE_NAME(RAW file name2)=240924_sf147.raw; RAW_FILE_NAME(mzXML file name)=240924_sf147.mzXML; Sex=Fs; Breed=Australische Herdershond; Age=6; Weight=28.3; AgeGroup=senior; BCS=7
SUBJECT_SAMPLE_FACTORS           	29	Dog29T3	Sample source:plasma | Diet:MCT | Timepoint:3	RAW_FILE_NAME(RAW file name2)=240924_sf026.raw; RAW_FILE_NAME(mzXML file name)=240924_sf026.mzXML; Sex=Ma; Breed=Poedel Middenslag; Age=7; Weight=5.1; AgeGroup=adult; BCS=6
SUBJECT_SAMPLE_FACTORS           	29	Dog29T0	Sample source:plasma | Diet:MCT | Timepoint:0	RAW_FILE_NAME(RAW file name2)=240924_sf096.raw; RAW_FILE_NAME(mzXML file name)=240924_sf096.mzXML; Sex=Ma; Breed=Poedel Middenslag; Age=7; Weight=5.1; AgeGroup=adult; BCS=6
SUBJECT_SAMPLE_FACTORS           	30	Dog30T3	Sample source:plasma | Diet:Pl | Timepoint:3	RAW_FILE_NAME(RAW file name2)=240924_sf022.raw; RAW_FILE_NAME(mzXML file name)=240924_sf022.mzXML; Sex=Ms; Breed=Yorkshire Terrier; Age=9; Weight=7.5; AgeGroup=senior; BCS=6
SUBJECT_SAMPLE_FACTORS           	30	Dog30T0	Sample source:plasma | Diet:Pl | Timepoint:0	RAW_FILE_NAME(RAW file name2)=240924_sf118.raw; RAW_FILE_NAME(mzXML file name)=240924_sf118.mzXML; Sex=Ms; Breed=Yorkshire Terrier; Age=9; Weight=7.5; AgeGroup=senior; BCS=6
SUBJECT_SAMPLE_FACTORS           	33	Dog33T0	Sample source:plasma | Diet:Pl | Timepoint:0	RAW_FILE_NAME(RAW file name2)=240924_sf110.raw; RAW_FILE_NAME(mzXML file name)=240924_sf110.mzXML; Sex=Ma; Breed=Teckel; Age=5; Weight=10.9; AgeGroup=adult; BCS=5
SUBJECT_SAMPLE_FACTORS           	33	Dog33T3	Sample source:plasma | Diet:Pl | Timepoint:3	RAW_FILE_NAME(RAW file name2)=240924_sf152.raw; RAW_FILE_NAME(mzXML file name)=240924_sf152.mzXML; Sex=Ma; Breed=Teckel; Age=5; Weight=10.9; AgeGroup=adult; BCS=5
SUBJECT_SAMPLE_FACTORS           	34	Dog34T0	Sample source:plasma | Diet:Pl | Timepoint:0	RAW_FILE_NAME(RAW file name2)=240924_sf104.raw; RAW_FILE_NAME(mzXML file name)=240924_sf104.mzXML; Sex=Ms; Breed=Border Collie; Age=2; Weight=24.5; AgeGroup=adult; BCS=6
SUBJECT_SAMPLE_FACTORS           	34	Dog34T3	Sample source:plasma | Diet:Pl | Timepoint:3	RAW_FILE_NAME(RAW file name2)=240924_sf138.raw; RAW_FILE_NAME(mzXML file name)=240924_sf138.mzXML; Sex=Ms; Breed=Border Collie; Age=2; Weight=24.5; AgeGroup=adult; BCS=6
SUBJECT_SAMPLE_FACTORS           	35	Dog35T0	Sample source:plasma | Diet:MCT | Timepoint:0	RAW_FILE_NAME(RAW file name2)=240924_sf063.raw; RAW_FILE_NAME(mzXML file name)=240924_sf063.mzXML; Sex=Fs; Breed=CV; Age=2; Weight=8.7; AgeGroup=adult; BCS=6
SUBJECT_SAMPLE_FACTORS           	35	Dog35T3	Sample source:plasma | Diet:MCT | Timepoint:3	RAW_FILE_NAME(RAW file name2)=240924_sf163.raw; RAW_FILE_NAME(mzXML file name)=240924_sf163.mzXML; Sex=Fs; Breed=CV; Age=2; Weight=8.7; AgeGroup=adult; BCS=6
SUBJECT_SAMPLE_FACTORS           	37	Dog37T3	Sample source:plasma | Diet:MCT | Timepoint:3	RAW_FILE_NAME(RAW file name2)=240924_sf034.raw; RAW_FILE_NAME(mzXML file name)=240924_sf034.mzXML; Sex=Ma; Breed=Schnauzer middenslag; Age=4; Weight=23; AgeGroup=adult; BCS=6
SUBJECT_SAMPLE_FACTORS           	37	Dog37T0	Sample source:plasma | Diet:MCT | Timepoint:0	RAW_FILE_NAME(RAW file name2)=240924_sf078.raw; RAW_FILE_NAME(mzXML file name)=240924_sf078.mzXML; Sex=Ma; Breed=Schnauzer middenslag; Age=4; Weight=23; AgeGroup=adult; BCS=6
SUBJECT_SAMPLE_FACTORS           	38	Dog38T0	Sample source:plasma | Diet:MCT | Timepoint:0	RAW_FILE_NAME(RAW file name2)=240924_sf076.raw; RAW_FILE_NAME(mzXML file name)=240924_sf076.mzXML; Sex=Fe; Breed=American Staffordshire terrier; Age=2; Weight=30; AgeGroup=adult; BCS=5
SUBJECT_SAMPLE_FACTORS           	38	Dog38T3	Sample source:plasma | Diet:MCT | Timepoint:3	RAW_FILE_NAME(RAW file name2)=240924_sf149.raw; RAW_FILE_NAME(mzXML file name)=240924_sf149.mzXML; Sex=Fe; Breed=American Staffordshire terrier; Age=2; Weight=30; AgeGroup=adult; BCS=5
SUBJECT_SAMPLE_FACTORS           	39	Dog39T3	Sample source:plasma | Diet:Pl | Timepoint:3	RAW_FILE_NAME(RAW file name2)=240924_sf053.raw; RAW_FILE_NAME(mzXML file name)=240924_sf053.mzXML; Sex=Fs; Breed=Vizsla; Age=1; Weight=15.4; AgeGroup=adult; BCS=3
SUBJECT_SAMPLE_FACTORS           	39	Dog39T0	Sample source:plasma | Diet:Pl | Timepoint:0	RAW_FILE_NAME(RAW file name2)=240924_sf098.raw; RAW_FILE_NAME(mzXML file name)=240924_sf098.mzXML; Sex=Fs; Breed=Vizsla; Age=1; Weight=15.4; AgeGroup=adult; BCS=3
SUBJECT_SAMPLE_FACTORS           	42	Dog42T3	Sample source:plasma | Diet:Pl | Timepoint:3	RAW_FILE_NAME(RAW file name2)=240924_sf037.raw; RAW_FILE_NAME(mzXML file name)=240924_sf037.mzXML; Sex=Ma; Breed=Beagle; Age=2; Weight=21.5; AgeGroup=adult; BCS=6
SUBJECT_SAMPLE_FACTORS           	42	Dog42T0	Sample source:plasma | Diet:Pl | Timepoint:0	RAW_FILE_NAME(RAW file name2)=240924_sf050.raw; RAW_FILE_NAME(mzXML file name)=240924_sf050.mzXML; Sex=Ma; Breed=Beagle; Age=2; Weight=21.5; AgeGroup=adult; BCS=6
SUBJECT_SAMPLE_FACTORS           	blank	blank1	Sample source:solvent | Diet:- | Timepoint:-	RAW_FILE_NAME(RAW file name2)=240924_sf032_Blank.raw; RAW_FILE_NAME(mzXML file name)=240924_sf032_Blank.mzXML; Sex=-; Breed=-; Age=-; Weight=-; AgeGroup=-; BCS=-
SUBJECT_SAMPLE_FACTORS           	blank	blank2	Sample source:solvent | Diet:- | Timepoint:-	RAW_FILE_NAME(RAW file name2)=240924_sf046_Blank.raw; RAW_FILE_NAME(mzXML file name)=240924_sf046_Blank.mzXML; Sex=-; Breed=-; Age=-; Weight=-; AgeGroup=-; BCS=-
SUBJECT_SAMPLE_FACTORS           	blank	blank3	Sample source:solvent | Diet:- | Timepoint:-	RAW_FILE_NAME(RAW file name2)=240924_sf060_Blank.raw; RAW_FILE_NAME(mzXML file name)=240924_sf060_Blank.mzXML; Sex=-; Breed=-; Age=-; Weight=-; AgeGroup=-; BCS=-
SUBJECT_SAMPLE_FACTORS           	blank	blank4	Sample source:solvent | Diet:- | Timepoint:-	RAW_FILE_NAME(RAW file name2)=240924_sf074_Blank.raw; RAW_FILE_NAME(mzXML file name)=240924_sf074_Blank.mzXML; Sex=-; Breed=-; Age=-; Weight=-; AgeGroup=-; BCS=-
SUBJECT_SAMPLE_FACTORS           	blank	blank5	Sample source:solvent | Diet:- | Timepoint:-	RAW_FILE_NAME(RAW file name2)=240924_sf088_Blank.raw; RAW_FILE_NAME(mzXML file name)=240924_sf088_Blank.mzXML; Sex=-; Breed=-; Age=-; Weight=-; AgeGroup=-; BCS=-
SUBJECT_SAMPLE_FACTORS           	blank	blank6	Sample source:solvent | Diet:- | Timepoint:-	RAW_FILE_NAME(RAW file name2)=240924_sf102_Blank.raw; RAW_FILE_NAME(mzXML file name)=240924_sf102_Blank.mzXML; Sex=-; Breed=-; Age=-; Weight=-; AgeGroup=-; BCS=-
SUBJECT_SAMPLE_FACTORS           	blank	blank7	Sample source:solvent | Diet:- | Timepoint:-	RAW_FILE_NAME(RAW file name2)=240924_sf116_Blank.raw; RAW_FILE_NAME(mzXML file name)=240924_sf116_Blank.mzXML; Sex=-; Breed=-; Age=-; Weight=-; AgeGroup=-; BCS=-
SUBJECT_SAMPLE_FACTORS           	blank	blank8	Sample source:solvent | Diet:- | Timepoint:-	RAW_FILE_NAME(RAW file name2)=240924_sf130_Blank.raw; RAW_FILE_NAME(mzXML file name)=240924_sf130_Blank.mzXML; Sex=-; Breed=-; Age=-; Weight=-; AgeGroup=-; BCS=-
SUBJECT_SAMPLE_FACTORS           	blank	blank9	Sample source:solvent | Diet:- | Timepoint:-	RAW_FILE_NAME(RAW file name2)=240924_sf144_Blank.raw; RAW_FILE_NAME(mzXML file name)=240924_sf144_Blank.mzXML; Sex=-; Breed=-; Age=-; Weight=-; AgeGroup=-; BCS=-
SUBJECT_SAMPLE_FACTORS           	blank	blank10	Sample source:solvent | Diet:- | Timepoint:-	RAW_FILE_NAME(RAW file name2)=240924_sf158_Blank.raw; RAW_FILE_NAME(mzXML file name)=240924_sf158_Blank.mzXML; Sex=-; Breed=-; Age=-; Weight=-; AgeGroup=-; BCS=-
SUBJECT_SAMPLE_FACTORS           	QC1	QC01	Sample source:pool | Diet:- | Timepoint:-	RAW_FILE_NAME(RAW file name2)=240924_sf016_QC0_1.raw; RAW_FILE_NAME(mzXML file name)=240924_sf016_QC0_1.mzXML; Sex=-; Breed=-; Age=-; Weight=-; AgeGroup=-; BCS=-
SUBJECT_SAMPLE_FACTORS           	QC1	QC02	Sample source:pool | Diet:- | Timepoint:-	RAW_FILE_NAME(RAW file name2)=240924_sf017_QC0_2.raw; RAW_FILE_NAME(mzXML file name)=240924_sf017_QC0_2.mzXML; Sex=-; Breed=-; Age=-; Weight=-; AgeGroup=-; BCS=-
SUBJECT_SAMPLE_FACTORS           	QC1	QC03	Sample source:pool | Diet:- | Timepoint:-	RAW_FILE_NAME(RAW file name2)=240924_sf018_QC0_3.raw; RAW_FILE_NAME(mzXML file name)=240924_sf018_QC0_3.mzXML; Sex=-; Breed=-; Age=-; Weight=-; AgeGroup=-; BCS=-
SUBJECT_SAMPLE_FACTORS           	QC1	QC04	Sample source:pool | Diet:- | Timepoint:-	RAW_FILE_NAME(RAW file name2)=240924_sf019_QC0_4.raw; RAW_FILE_NAME(mzXML file name)=240924_sf019_QC0_4.mzXML; Sex=-; Breed=-; Age=-; Weight=-; AgeGroup=-; BCS=-
SUBJECT_SAMPLE_FACTORS           	QC1	QC11	Sample source:pool | Diet:- | Timepoint:-	RAW_FILE_NAME(RAW file name2)=240924_sf030_QC1_1.raw; RAW_FILE_NAME(mzXML file name)=240924_sf030_QC1_1.mzXML; Sex=-; Breed=-; Age=-; Weight=-; AgeGroup=-; BCS=-
SUBJECT_SAMPLE_FACTORS           	QC1	QC13	Sample source:pool | Diet:- | Timepoint:-	RAW_FILE_NAME(RAW file name2)=240924_sf033_QC1_3.raw; RAW_FILE_NAME(mzXML file name)=240924_sf033_QC1_3.mzXML; Sex=-; Breed=-; Age=-; Weight=-; AgeGroup=-; BCS=-
SUBJECT_SAMPLE_FACTORS           	QC1	QC21	Sample source:pool | Diet:- | Timepoint:-	RAW_FILE_NAME(RAW file name2)=240924_sf044_QC2_1.raw; RAW_FILE_NAME(mzXML file name)=240924_sf044_QC2_1.mzXML; Sex=-; Breed=-; Age=-; Weight=-; AgeGroup=-; BCS=-
SUBJECT_SAMPLE_FACTORS           	QC1	QC23	Sample source:pool | Diet:- | Timepoint:-	RAW_FILE_NAME(RAW file name2)=240924_sf047_QC2_3.raw; RAW_FILE_NAME(mzXML file name)=240924_sf047_QC2_3.mzXML; Sex=-; Breed=-; Age=-; Weight=-; AgeGroup=-; BCS=-
SUBJECT_SAMPLE_FACTORS           	QC1	QC31	Sample source:pool | Diet:- | Timepoint:-	RAW_FILE_NAME(RAW file name2)=240924_sf058_QC3_1.raw; RAW_FILE_NAME(mzXML file name)=240924_sf058_QC3_1.mzXML; Sex=-; Breed=-; Age=-; Weight=-; AgeGroup=-; BCS=-
SUBJECT_SAMPLE_FACTORS           	QC1	QC33	Sample source:pool | Diet:- | Timepoint:-	RAW_FILE_NAME(RAW file name2)=240924_sf061_QC3_3.raw; RAW_FILE_NAME(mzXML file name)=240924_sf061_QC3_3.mzXML; Sex=-; Breed=-; Age=-; Weight=-; AgeGroup=-; BCS=-
SUBJECT_SAMPLE_FACTORS           	QC1	QC41	Sample source:pool | Diet:- | Timepoint:-	RAW_FILE_NAME(RAW file name2)=240924_sf072_QC4_1.raw; RAW_FILE_NAME(mzXML file name)=240924_sf072_QC4_1.mzXML; Sex=-; Breed=-; Age=-; Weight=-; AgeGroup=-; BCS=-
SUBJECT_SAMPLE_FACTORS           	QC1	QC43	Sample source:pool | Diet:- | Timepoint:-	RAW_FILE_NAME(RAW file name2)=240924_sf075_QC4_3.raw; RAW_FILE_NAME(mzXML file name)=240924_sf075_QC4_3.mzXML; Sex=-; Breed=-; Age=-; Weight=-; AgeGroup=-; BCS=-
SUBJECT_SAMPLE_FACTORS           	QC1	QC51	Sample source:pool | Diet:- | Timepoint:-	RAW_FILE_NAME(RAW file name2)=240924_sf086_QC5_1.raw; RAW_FILE_NAME(mzXML file name)=240924_sf086_QC5_1.mzXML; Sex=-; Breed=-; Age=-; Weight=-; AgeGroup=-; BCS=-
SUBJECT_SAMPLE_FACTORS           	QC1	QC53	Sample source:pool | Diet:- | Timepoint:-	RAW_FILE_NAME(RAW file name2)=240924_sf089_QC5_3.raw; RAW_FILE_NAME(mzXML file name)=240924_sf089_QC5_3.mzXML; Sex=-; Breed=-; Age=-; Weight=-; AgeGroup=-; BCS=-
SUBJECT_SAMPLE_FACTORS           	QC1	QC61	Sample source:pool | Diet:- | Timepoint:-	RAW_FILE_NAME(RAW file name2)=240924_sf100_QC6_1.raw; RAW_FILE_NAME(mzXML file name)=240924_sf100_QC6_1.mzXML; Sex=-; Breed=-; Age=-; Weight=-; AgeGroup=-; BCS=-
SUBJECT_SAMPLE_FACTORS           	QC1	QC63	Sample source:pool | Diet:- | Timepoint:-	RAW_FILE_NAME(RAW file name2)=240924_sf103_QC6_3.raw; RAW_FILE_NAME(mzXML file name)=240924_sf103_QC6_3.mzXML; Sex=-; Breed=-; Age=-; Weight=-; AgeGroup=-; BCS=-
SUBJECT_SAMPLE_FACTORS           	QC1	QC71	Sample source:pool | Diet:- | Timepoint:-	RAW_FILE_NAME(RAW file name2)=240924_sf114_QC7_1.raw; RAW_FILE_NAME(mzXML file name)=240924_sf114_QC7_1.mzXML; Sex=-; Breed=-; Age=-; Weight=-; AgeGroup=-; BCS=-
SUBJECT_SAMPLE_FACTORS           	QC1	QC73	Sample source:pool | Diet:- | Timepoint:-	RAW_FILE_NAME(RAW file name2)=240924_sf117_QC7_3.raw; RAW_FILE_NAME(mzXML file name)=240924_sf117_QC7_3.mzXML; Sex=-; Breed=-; Age=-; Weight=-; AgeGroup=-; BCS=-
SUBJECT_SAMPLE_FACTORS           	QC1	QC81	Sample source:pool | Diet:- | Timepoint:-	RAW_FILE_NAME(RAW file name2)=240924_sf128_QC8_1.raw; RAW_FILE_NAME(mzXML file name)=240924_sf128_QC8_1.mzXML; Sex=-; Breed=-; Age=-; Weight=-; AgeGroup=-; BCS=-
SUBJECT_SAMPLE_FACTORS           	QC1	QC83	Sample source:pool | Diet:- | Timepoint:-	RAW_FILE_NAME(RAW file name2)=240924_sf131_QC8_3.raw; RAW_FILE_NAME(mzXML file name)=240924_sf131_QC8_3.mzXML; Sex=-; Breed=-; Age=-; Weight=-; AgeGroup=-; BCS=-
SUBJECT_SAMPLE_FACTORS           	QC1	QC91	Sample source:pool | Diet:- | Timepoint:-	RAW_FILE_NAME(RAW file name2)=240924_sf142_QC9_1.raw; RAW_FILE_NAME(mzXML file name)=240924_sf142_QC9_1.mzXML; Sex=-; Breed=-; Age=-; Weight=-; AgeGroup=-; BCS=-
SUBJECT_SAMPLE_FACTORS           	QC1	QC93	Sample source:pool | Diet:- | Timepoint:-	RAW_FILE_NAME(RAW file name2)=240924_sf145_QC9_3.raw; RAW_FILE_NAME(mzXML file name)=240924_sf145_QC9_3.mzXML; Sex=-; Breed=-; Age=-; Weight=-; AgeGroup=-; BCS=-
SUBJECT_SAMPLE_FACTORS           	QC1	QC101	Sample source:pool | Diet:- | Timepoint:-	RAW_FILE_NAME(RAW file name2)=240924_sf156_QC10_1.raw; RAW_FILE_NAME(mzXML file name)=240924_sf156_QC10_1.mzXML; Sex=-; Breed=-; Age=-; Weight=-; AgeGroup=-; BCS=-
SUBJECT_SAMPLE_FACTORS           	QC1	QC103	Sample source:pool | Diet:- | Timepoint:-	RAW_FILE_NAME(RAW file name2)=240924_sf159_QC10_3.raw; RAW_FILE_NAME(mzXML file name)=240924_sf159_QC10_3.mzXML; Sex=-; Breed=-; Age=-; Weight=-; AgeGroup=-; BCS=-
SUBJECT_SAMPLE_FACTORS           	QC1	QC111	Sample source:pool | Diet:- | Timepoint:-	RAW_FILE_NAME(RAW file name2)=240924_sf164_QC11_1.raw; RAW_FILE_NAME(mzXML file name)=240924_sf164_QC11_1.mzXML; Sex=-; Breed=-; Age=-; Weight=-; AgeGroup=-; BCS=-
SUBJECT_SAMPLE_FACTORS           	QC2	QC12	Sample source:pool | Diet:- | Timepoint:-	RAW_FILE_NAME(RAW file name2)=240924_sf031_QC1_2.raw; RAW_FILE_NAME(mzXML file name)=240924_sf031_QC1_2.mzXML; Sex=-; Breed=-; Age=-; Weight=-; AgeGroup=-; BCS=-
SUBJECT_SAMPLE_FACTORS           	QC2	QC22	Sample source:pool | Diet:- | Timepoint:-	RAW_FILE_NAME(RAW file name2)=240924_sf045_QC2_2.raw; RAW_FILE_NAME(mzXML file name)=240924_sf045_QC2_2.mzXML; Sex=-; Breed=-; Age=-; Weight=-; AgeGroup=-; BCS=-
SUBJECT_SAMPLE_FACTORS           	QC2	QC32	Sample source:pool | Diet:- | Timepoint:-	RAW_FILE_NAME(RAW file name2)=240924_sf059_QC3_2.raw; RAW_FILE_NAME(mzXML file name)=240924_sf059_QC3_2.mzXML; Sex=-; Breed=-; Age=-; Weight=-; AgeGroup=-; BCS=-
SUBJECT_SAMPLE_FACTORS           	QC2	QC42	Sample source:pool | Diet:- | Timepoint:-	RAW_FILE_NAME(RAW file name2)=240924_sf073_QC4_2.raw; RAW_FILE_NAME(mzXML file name)=240924_sf073_QC4_2.mzXML; Sex=-; Breed=-; Age=-; Weight=-; AgeGroup=-; BCS=-
SUBJECT_SAMPLE_FACTORS           	QC2	QC52	Sample source:pool | Diet:- | Timepoint:-	RAW_FILE_NAME(RAW file name2)=240924_sf087_QC5_2.raw; RAW_FILE_NAME(mzXML file name)=240924_sf087_QC5_2.mzXML; Sex=-; Breed=-; Age=-; Weight=-; AgeGroup=-; BCS=-
SUBJECT_SAMPLE_FACTORS           	QC2	QC62	Sample source:pool | Diet:- | Timepoint:-	RAW_FILE_NAME(RAW file name2)=240924_sf101_QC6_2.raw; RAW_FILE_NAME(mzXML file name)=240924_sf101_QC6_2.mzXML; Sex=-; Breed=-; Age=-; Weight=-; AgeGroup=-; BCS=-
SUBJECT_SAMPLE_FACTORS           	QC2	QC72	Sample source:pool | Diet:- | Timepoint:-	RAW_FILE_NAME(RAW file name2)=240924_sf115_QC7_2.raw; RAW_FILE_NAME(mzXML file name)=240924_sf115_QC7_2.mzXML; Sex=-; Breed=-; Age=-; Weight=-; AgeGroup=-; BCS=-
SUBJECT_SAMPLE_FACTORS           	QC2	QC82	Sample source:pool | Diet:- | Timepoint:-	RAW_FILE_NAME(RAW file name2)=240924_sf129_QC8_2.raw; RAW_FILE_NAME(mzXML file name)=240924_sf129_QC8_2.mzXML; Sex=-; Breed=-; Age=-; Weight=-; AgeGroup=-; BCS=-
SUBJECT_SAMPLE_FACTORS           	QC2	QC92	Sample source:pool | Diet:- | Timepoint:-	RAW_FILE_NAME(RAW file name2)=240924_sf143_QC9_2.raw; RAW_FILE_NAME(mzXML file name)=240924_sf143_QC9_2.mzXML; Sex=-; Breed=-; Age=-; Weight=-; AgeGroup=-; BCS=-
SUBJECT_SAMPLE_FACTORS           	QC2	QC102	Sample source:pool | Diet:- | Timepoint:-	RAW_FILE_NAME(RAW file name2)=240924_sf157_QC10_2.raw; RAW_FILE_NAME(mzXML file name)=240924_sf157_QC10_2.mzXML; Sex=-; Breed=-; Age=-; Weight=-; AgeGroup=-; BCS=-
SUBJECT_SAMPLE_FACTORS           	QC2	QC112	Sample source:pool | Diet:- | Timepoint:-	RAW_FILE_NAME(RAW file name2)=240924_sf165_QC11_2.raw; RAW_FILE_NAME(mzXML file name)=240924_sf165_QC11_2.mzXML; Sex=-; Breed=-; Age=-; Weight=-; AgeGroup=-; BCS=-
SUBJECT_SAMPLE_FACTORS           	STD 1	STD 1.0	Sample source:standard mixture | Diet:- | Timepoint:-	RAW_FILE_NAME(RAW file name2)=240924_sf002_std1.raw; RAW_FILE_NAME(mzXML file name)=240924_sf002_std1.mzXML; Sex=-; Breed=-; Age=-; Weight=-; AgeGroup=-; BCS=-
SUBJECT_SAMPLE_FACTORS           	STD 1	STD 1.1	Sample source:standard mixture | Diet:- | Timepoint:-	RAW_FILE_NAME(RAW file name2)=240924_sf003_std1.raw; RAW_FILE_NAME(mzXML file name)=240924_sf003_std1.mzXML; Sex=-; Breed=-; Age=-; Weight=-; AgeGroup=-; BCS=-
SUBJECT_SAMPLE_FACTORS           	STD 1	STD 1.2	Sample source:standard mixture | Diet:- | Timepoint:-	RAW_FILE_NAME(RAW file name2)=240924_sf166_std1.raw; RAW_FILE_NAME(mzXML file name)=240924_sf166_std1.mzXML; Sex=-; Breed=-; Age=-; Weight=-; AgeGroup=-; BCS=-
SUBJECT_SAMPLE_FACTORS           	STD 10	STD 10.1	Sample source:standard mixture | Diet:- | Timepoint:-	RAW_FILE_NAME(RAW file name2)=240924_sf012_std10.raw; RAW_FILE_NAME(mzXML file name)=240924_sf012_std10.mzXML; Sex=-; Breed=-; Age=-; Weight=-; AgeGroup=-; BCS=-
SUBJECT_SAMPLE_FACTORS           	STD 10	STD 10.2	Sample source:standard mixture | Diet:- | Timepoint:-	RAW_FILE_NAME(RAW file name2)=240924_sf175_std10.raw; RAW_FILE_NAME(mzXML file name)=240924_sf175_std10.mzXML; Sex=-; Breed=-; Age=-; Weight=-; AgeGroup=-; BCS=-
SUBJECT_SAMPLE_FACTORS           	STD 2	STD 2.1	Sample source:standard mixture | Diet:- | Timepoint:-	RAW_FILE_NAME(RAW file name2)=240924_sf004_std2.raw; RAW_FILE_NAME(mzXML file name)=240924_sf004_std2.mzXML; Sex=-; Breed=-; Age=-; Weight=-; AgeGroup=-; BCS=-
SUBJECT_SAMPLE_FACTORS           	STD 2	STD 2.2	Sample source:standard mixture | Diet:- | Timepoint:-	RAW_FILE_NAME(RAW file name2)=240924_sf167_std2.raw; RAW_FILE_NAME(mzXML file name)=240924_sf167_std2.mzXML; Sex=-; Breed=-; Age=-; Weight=-; AgeGroup=-; BCS=-
SUBJECT_SAMPLE_FACTORS           	STD 3	STD 3.1	Sample source:standard mixture | Diet:- | Timepoint:-	RAW_FILE_NAME(RAW file name2)=240924_sf005_std3.raw; RAW_FILE_NAME(mzXML file name)=240924_sf005_std3.mzXML; Sex=-; Breed=-; Age=-; Weight=-; AgeGroup=-; BCS=-
SUBJECT_SAMPLE_FACTORS           	STD 3	STD 3.2	Sample source:standard mixture | Diet:- | Timepoint:-	RAW_FILE_NAME(RAW file name2)=240924_sf168_std3.raw; RAW_FILE_NAME(mzXML file name)=240924_sf168_std3.mzXML; Sex=-; Breed=-; Age=-; Weight=-; AgeGroup=-; BCS=-
SUBJECT_SAMPLE_FACTORS           	STD 4	STD 4.1	Sample source:standard mixture | Diet:- | Timepoint:-	RAW_FILE_NAME(RAW file name2)=240924_sf006_std4.raw; RAW_FILE_NAME(mzXML file name)=240924_sf006_std4.mzXML; Sex=-; Breed=-; Age=-; Weight=-; AgeGroup=-; BCS=-
SUBJECT_SAMPLE_FACTORS           	STD 4	STD 4.2	Sample source:standard mixture | Diet:- | Timepoint:-	RAW_FILE_NAME(RAW file name2)=240924_sf169_std4.raw; RAW_FILE_NAME(mzXML file name)=240924_sf169_std4.mzXML; Sex=-; Breed=-; Age=-; Weight=-; AgeGroup=-; BCS=-
SUBJECT_SAMPLE_FACTORS           	STD 5	STD 5.1	Sample source:standard mixture | Diet:- | Timepoint:-	RAW_FILE_NAME(RAW file name2)=240924_sf007_std5.raw; RAW_FILE_NAME(mzXML file name)=240924_sf007_std5.mzXML; Sex=-; Breed=-; Age=-; Weight=-; AgeGroup=-; BCS=-
SUBJECT_SAMPLE_FACTORS           	STD 5	STD 5.2	Sample source:standard mixture | Diet:- | Timepoint:-	RAW_FILE_NAME(RAW file name2)=240924_sf170_std5.raw; RAW_FILE_NAME(mzXML file name)=240924_sf170_std5.mzXML; Sex=-; Breed=-; Age=-; Weight=-; AgeGroup=-; BCS=-
SUBJECT_SAMPLE_FACTORS           	STD 6	STD 6.1	Sample source:standard mixture | Diet:- | Timepoint:-	RAW_FILE_NAME(RAW file name2)=240924_sf008_std6.raw; RAW_FILE_NAME(mzXML file name)=240924_sf008_std6.mzXML; Sex=-; Breed=-; Age=-; Weight=-; AgeGroup=-; BCS=-
SUBJECT_SAMPLE_FACTORS           	STD 6	STD 6.2	Sample source:standard mixture | Diet:- | Timepoint:-	RAW_FILE_NAME(RAW file name2)=240924_sf171_std6.raw; RAW_FILE_NAME(mzXML file name)=240924_sf171_std6.mzXML; Sex=-; Breed=-; Age=-; Weight=-; AgeGroup=-; BCS=-
SUBJECT_SAMPLE_FACTORS           	STD 7	STD 7.1	Sample source:standard mixture | Diet:- | Timepoint:-	RAW_FILE_NAME(RAW file name2)=240924_sf009_std7.raw; RAW_FILE_NAME(mzXML file name)=240924_sf009_std7.mzXML; Sex=-; Breed=-; Age=-; Weight=-; AgeGroup=-; BCS=-
SUBJECT_SAMPLE_FACTORS           	STD 7	STD 7.2	Sample source:standard mixture | Diet:- | Timepoint:-	RAW_FILE_NAME(RAW file name2)=240924_sf172_std7.raw; RAW_FILE_NAME(mzXML file name)=240924_sf172_std7.mzXML; Sex=-; Breed=-; Age=-; Weight=-; AgeGroup=-; BCS=-
SUBJECT_SAMPLE_FACTORS           	STD 8	STD 8.1	Sample source:standard mixture | Diet:- | Timepoint:-	RAW_FILE_NAME(RAW file name2)=240924_sf010_std8.raw; RAW_FILE_NAME(mzXML file name)=240924_sf010_std8.mzXML; Sex=-; Breed=-; Age=-; Weight=-; AgeGroup=-; BCS=-
SUBJECT_SAMPLE_FACTORS           	STD 8	STD 8.2	Sample source:standard mixture | Diet:- | Timepoint:-	RAW_FILE_NAME(RAW file name2)=240924_sf173_std8.raw; RAW_FILE_NAME(mzXML file name)=240924_sf173_std8.mzXML; Sex=-; Breed=-; Age=-; Weight=-; AgeGroup=-; BCS=-
SUBJECT_SAMPLE_FACTORS           	STD 9	STD 9.1	Sample source:standard mixture | Diet:- | Timepoint:-	RAW_FILE_NAME(RAW file name2)=240924_sf011_std9.raw; RAW_FILE_NAME(mzXML file name)=240924_sf011_std9.mzXML; Sex=-; Breed=-; Age=-; Weight=-; AgeGroup=-; BCS=-
SUBJECT_SAMPLE_FACTORS           	STD 9	STD 9.2	Sample source:standard mixture | Diet:- | Timepoint:-	RAW_FILE_NAME(RAW file name2)=240924_sf174_std9.raw; RAW_FILE_NAME(mzXML file name)=240924_sf174_std9.mzXML; Sex=-; Breed=-; Age=-; Weight=-; AgeGroup=-; BCS=-
SUBJECT_SAMPLE_FACTORS           	STD epi	STD epi.1	Sample source:standard mixture | Diet:- | Timepoint:-	RAW_FILE_NAME(RAW file name2)=240924_sf014_stdEpi.raw; RAW_FILE_NAME(mzXML file name)=240924_sf014_stdEpi.mzXML; Sex=-; Breed=-; Age=-; Weight=-; AgeGroup=-; BCS=-
SUBJECT_SAMPLE_FACTORS           	STD epi	STD epi.2	Sample source:standard mixture | Diet:- | Timepoint:-	RAW_FILE_NAME(RAW file name2)=240924_sf177_stdEpi.raw; RAW_FILE_NAME(mzXML file name)=240924_sf177_stdEpi.mzXML; Sex=-; Breed=-; Age=-; Weight=-; AgeGroup=-; BCS=-
SUBJECT_SAMPLE_FACTORS           	STD Fien	STD Fien.1	Sample source:standard mixture | Diet:- | Timepoint:-	RAW_FILE_NAME(RAW file name2)=240924_sf013_stdF.raw; RAW_FILE_NAME(mzXML file name)=240924_sf013_stdF.mzXML; Sex=-; Breed=-; Age=-; Weight=-; AgeGroup=-; BCS=-
SUBJECT_SAMPLE_FACTORS           	STD Fien	STD Fien.2	Sample source:standard mixture | Diet:- | Timepoint:-	RAW_FILE_NAME(RAW file name2)=240924_sf176_stdF.raw; RAW_FILE_NAME(mzXML file name)=240924_sf176_stdF.mzXML; Sex=-; Breed=-; Age=-; Weight=-; AgeGroup=-; BCS=-
#COLLECTION
CO:COLLECTION_SUMMARY            	Pre-prandial plasma samples were collected at T0 and T3, after an 8-hour fast,
CO:COLLECTION_SUMMARY            	centrifuged, aliquoted and flash frozen using liquid nitrogen, prior to storage
CO:COLLECTION_SUMMARY            	at -80°C until metabolome analysis. T0 = Before the start of the
CO:COLLECTION_SUMMARY            	placebo-controlled dietary trial. All dogs did receive an identical, adult
CO:COLLECTION_SUMMARY            	maintenance diet for at least 21 days before the collection of T0 samples. T3 =
CO:COLLECTION_SUMMARY            	At the end of the placebo-controlled dietary trial, i.e. following 3 months on
CO:COLLECTION_SUMMARY            	either placebo or medium chain triglycerides (MCT) diet. (NOTE: Fe=female
CO:COLLECTION_SUMMARY            	entire, Fs=female spayed, Ma=male intact, Ms=male neutered; BCS=body condition
CO:COLLECTION_SUMMARY            	score 1-9)
CO:SAMPLE_TYPE                   	Blood (plasma)
CO:COLLECTION_METHOD             	Blood collection by veterinarian
CO:COLLECTION_LOCATION           	Small Animal Department - Ghent University - Merelbeke
CO:COLLECTION_FREQUENCY          	twice per dog (T0 and T3)
CO:STORAGE_CONDITIONS            	-80℃
CO:COLLECTION_VIALS              	Heparine
CO:STORAGE_VIALS                 	Cryotubes
#TREATMENT
TR:TREATMENT_SUMMARY             	Medium chain triglyceride diet OR isocaloric placebo diet (containing long chain
TR:TREATMENT_SUMMARY             	triglycerides)
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	To extract the polar plasma metabolome, 150 µl of plasma was mixed with 600 µl
SP:SAMPLEPREP_SUMMARY            	of methanol/acetonitril/aceton (1:1:1) solvens, after the addition of 10 µL
SP:SAMPLEPREP_SUMMARY            	ISTD mixture (20 ng/µl of tyrosine-d2, phenylalanine-d2, deoxycholic acid-d4,
SP:SAMPLEPREP_SUMMARY            	l-alanine-d3 and dopamine-d4). Subsequent to 2 min of thorough vortexing,
SP:SAMPLEPREP_SUMMARY            	proteins were precipitated for 30 min at 4°C.The supernatant of the
SP:SAMPLEPREP_SUMMARY            	liquid-liquid extraction was collected after a 10-min centrifugation step (15
SP:SAMPLEPREP_SUMMARY            	000 x g, at 4 %C). Next, the extract was evaporated to dryness using a
SP:SAMPLEPREP_SUMMARY            	N-evaporator. The residue was diluted in 200 µl of ultrapure water, vortexed
SP:SAMPLEPREP_SUMMARY            	for 30 sec and centrifugated(10 min, 15 000 x g, at 4°C), before transferring
SP:SAMPLEPREP_SUMMARY            	to a glass HPLCvial with insert.
SP:PROCESSING_STORAGE_CONDITIONS 	Described in summary
SP:EXTRACTION_METHOD             	liquid-liquid
SP:EXTRACT_STORAGE               	4℃
SP:SAMPLE_RESUSPENSION           	ultrapure water
SP:SAMPLE_SPIKING                	ISTD
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_SUMMARY        	Reversed phase Chromatography
CH:CHROMATOGRAPHY_TYPE           	Reversed phase
CH:INSTRUMENT_NAME               	Thermo Dionex Ultimate 3000
CH:COLUMN_NAME                   	Waters ACQUITY UPLC HSS T3 (150 x 2.1mm,1.8um)
CH:SOLVENT_A                     	100% water; 0.1% formic acid
CH:SOLVENT_B                     	100% acetonitrile; 0.1% formic acid
CH:FLOW_GRADIENT                 	A gradient profile with following proportions (v/v) of solvent A was applied:
CH:FLOW_GRADIENT                 	0e1.5 min at 98%, 1.5e7.0 min from 98% to 75%, 7.0e8.0 min from 75% to 40%,
CH:FLOW_GRADIENT                 	8.0e12.0 min from 40% to 5%, 12.0e14.0 min at 5%, 14.0e14.1 min from 5 to 98%,
CH:FLOW_GRADIENT                 	followed by 4.0 min of re-equilibration.
CH:FLOW_RATE                     	0.4 ml/min
CH:COLUMN_TEMPERATURE            	45
CH:METHODS_ID                    	CH003650
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
AN:LABORATORY_NAME               	LIMET
AN:ACQUISITION_DATE              	24/09/2024
#MS
MS:INSTRUMENT_NAME               	Thermo Q Exactive Orbitrap
MS:INSTRUMENT_TYPE               	Orbitrap
MS:MS_TYPE                       	ESI
MS:ION_MODE                      	NEGATIVE
MS:MS_COMMENTS                   	MS acquisition: Xcalibur, polarity switching mode, according to De Paepe et al,
MS:MS_COMMENTS                   	2018 Targeted data processing: TARDIS Untargeted data acquisition: Compound
MS:MS_COMMENTS                   	Discoverer, R studio
MS:CAPILLARY_VOLTAGE             	90 V
MS:IONIZATION                    	Negative
MS:SPRAY_VOLTAGE                 	- 5 kV
#MS_METABOLITE_DATA
MS_METABOLITE_DATA:UNITS	iQC normalized AUC
MS_METABOLITE_DATA_START
Samples	Dog26T0	Dog30T3	Dog1T3	Dog29T3	Dog17T3	Dog37T3	Dog7T0	Dog42T3	Dog21T3	Dog9T3	Dog4T0	Dog4T3	Dog42T0	Dog11T0	Dog39T3	Dog1T0	Dog18T3	Dog8T3	Dog25T0	Dog35T0	Dog10T0	Dog12T0	Dog28T3	Dog3T0	Dog20T0	Dog24T3	Dog27T3	Dog38T0	Dog5T0	Dog37T0	Dog24T0	Dog13T0	Dog18T0	Dog16T3	Dog26T3	Dog3T3	Dog29T0	Dog39T0	Dog34T0	Dog19T0	Dog33T0	Dog21T0	Dog30T0	Dog6T0	Dog8T0	Dog27T0	Dog12T3	Dog19T3	Dog9T0	Dog25T3	Dog10T3	Dog34T3	Dog5T3	Dog13T3	Dog11T3	Dog28T0	Dog17T0	Dog38T3	Dog16T0	Dog33T3	Dog6T3	Dog7T3	Dog20T3	Dog35T3
Factors	Sample source:plasma | Diet:Pl | Timepoint:0	Sample source:plasma | Diet:Pl | Timepoint:3	Sample source:plasma | Diet:MCT | Timepoint:3	Sample source:plasma | Diet:MCT | Timepoint:3	Sample source:plasma | Diet:Pl | Timepoint:3	Sample source:plasma | Diet:MCT | Timepoint:3	Sample source:plasma | Diet:MCT | Timepoint:0	Sample source:plasma | Diet:Pl | Timepoint:3	Sample source:plasma | Diet:Pl | Timepoint:3	Sample source:plasma | Diet:Pl | Timepoint:3	Sample source:plasma | Diet:Pl | Timepoint:0	Sample source:plasma | Diet:Pl | Timepoint:3	Sample source:plasma | Diet:Pl | Timepoint:0	Sample source:plasma | Diet:MCT | Timepoint:0	Sample source:plasma | Diet:Pl | Timepoint:3	Sample source:plasma | Diet:MCT | Timepoint:0	Sample source:plasma | Diet:MCT | Timepoint:3	Sample source:plasma | Diet:MCT | Timepoint:3	Sample source:plasma | Diet:MCT | Timepoint:0	Sample source:plasma | Diet:MCT | Timepoint:0	Sample source:plasma | Diet:MCT | Timepoint:0	Sample source:plasma | Diet:MCT | Timepoint:0	Sample source:plasma | Diet:Pl | Timepoint:3	Sample source:plasma | Diet:Pl | Timepoint:0	Sample source:plasma | Diet:MCT | Timepoint:0	Sample source:plasma | Diet:Pl | Timepoint:3	Sample source:plasma | Diet:MCT | Timepoint:3	Sample source:plasma | Diet:MCT | Timepoint:0	Sample source:plasma | Diet:Pl | Timepoint:0	Sample source:plasma | Diet:MCT | Timepoint:0	Sample source:plasma | Diet:Pl | Timepoint:0	Sample source:plasma | Diet:Pl | Timepoint:0	Sample source:plasma | Diet:MCT | Timepoint:0	Sample source:plasma | Diet:MCT | Timepoint:3	Sample source:plasma | Diet:Pl | Timepoint:3	Sample source:plasma | Diet:Pl | Timepoint:3	Sample source:plasma | Diet:MCT | Timepoint:0	Sample source:plasma | Diet:Pl | Timepoint:0	Sample source:plasma | Diet:Pl | Timepoint:0	Sample source:plasma | Diet:MCT | Timepoint:0	Sample source:plasma | Diet:Pl | Timepoint:0	Sample source:plasma | Diet:Pl | Timepoint:0	Sample source:plasma | Diet:Pl | Timepoint:0	Sample source:plasma | Diet:Pl | Timepoint:0	Sample source:plasma | Diet:MCT | Timepoint:0	Sample source:plasma | Diet:MCT | Timepoint:0	Sample source:plasma | Diet:MCT | Timepoint:3	Sample source:plasma | Diet:MCT | Timepoint:3	Sample source:plasma | Diet:Pl | Timepoint:0	Sample source:plasma | Diet:MCT | Timepoint:3	Sample source:plasma | Diet:MCT | Timepoint:3	Sample source:plasma | Diet:Pl | Timepoint:3	Sample source:plasma | Diet:Pl | Timepoint:3	Sample source:plasma | Diet:Pl | Timepoint:3	Sample source:plasma | Diet:MCT | Timepoint:3	Sample source:plasma | Diet:Pl | Timepoint:0	Sample source:plasma | Diet:Pl | Timepoint:0	Sample source:plasma | Diet:MCT | Timepoint:3	Sample source:plasma | Diet:MCT | Timepoint:0	Sample source:plasma | Diet:Pl | Timepoint:3	Sample source:plasma | Diet:Pl | Timepoint:3	Sample source:plasma | Diet:MCT | Timepoint:3	Sample source:plasma | Diet:MCT | Timepoint:3	Sample source:plasma | Diet:MCT | Timepoint:3
2-Hydroxyglutaric acid	1.042944592	0.878769773	1.007356991	0.762314058	1.966432357	0.856377249	0.971533019	1.087880474	1.565232007	1.554691311	2.277539117	0.937011227	1.035425607	1.577554918	0.958759022	1.231662419	0.845569957	1.00582684	1.553274433	0.954466996	1.471553697	0.994251826	0.941738868	1.732718963	0.852818898	1.291671293	1.20330692	1.399091978	2.319184531	1.108405518	0.941694431	1.629723567	2.688383017	1.113852944	0.979548383	2.458059999	0.618285611	1.162100077	1.376541902	0.902550474	1.166912388	0.821403412	0.951740369	1.265724413	0.816096231	1.472997093	0.913869284	1.026274394	1.236307372	1.075948764	0.765954588	1.063994342	1.442686891	0.906265051	1.30403476	0.965941653	1.676353454	1.158723411	1.995564391	1.201318394	1.468675112	0.927783935	0.802164771	0.760732745
3-Hydroxyisovaleric acid	0.878761539	0.970747046	0.721290163	0.759068046	0.823629233	1.045040326	0.758949907	1.396315916	0.413162955	1.149757586	3.012052939	0.714728697	1.229506995	0.811709317	0.92288322	0.657438868	0.880227023	1.62577646	1.474594283	0.789753909	2.524767641	0.585819899	1.076854591	0.571465544	0.792187406	0.636820014	1.315615067	0.676073663	2.412852419	0.989413064	1.341127248	1.760335711	2.245791371	0.706609381	0.997408313	0.404076871	0.87619148	0.90394123	8.702001307	8.391994671	0.504977936	0.451388324	1.05115636	0.96241704	1.430624191	1.165589419	0.737979432	8.345046927	1.235699288	1.287917071	1.06470881	9.479703028	0.78291882	0.908376337	0.777547085	0.89565848	0.687868125	0.863954257	2.056623088	0.411742297	0.959914023	0.685705795	0.806154738	0.769810419
4-Guanidinobutanoic acid	4.288029291	0.243131929	1.276773707	0.227130026	0.34690612	0.265454435	4.974005958	0.397601736	0.229265356	0.291593858	2.421758361	0.33858179	0.333352712	2.854145127	0.197294284	0.215853986	0.288025553	0.333740379	0.14431654	0.100124649	1.025600946	4.525166442	0.393812246	2.669450173	4.897845501	1.03807856	0.591870896	0.162303221	3.057479004	0.106708011	11.45388515	2.393074074	1.782702308	0.08626944	0.203128058	0.246698695	0.206146057	0.096472388	0.365674586	0.243775825	0.115766993	6.316371524	0.203668765	0.142837879	1.987009414	7.248131392	0.186625464	0.43685773	0.13885963	0.222172876	0.164675664	0.160572659	0.66740589	0.731738601	0.350239589	0.237292931	7.241266907	0.465851143	0.807741497	0.283706326	0.389906858	0.45598149	0.39377065	0.339326964
Cholic acid	2.99308976	0.004706157	0.043048155	0.076761907	0.004706157	0.004592816	3.055416294	1.158390391	0.712575207	9.563696264	1.029907764	0.200461487	0.004354222	0.152997174	0.102943302	0.415695652	0.033403131	0.605621227	0.004604078	0.243749636	0.028547332	0.004604078	0.004604078	0.526631801	0.440760685	0.226200736	15.23269985	0.044694879	0.52137003	0.281457038	0.00421649	0.273173685	1.736446181	0.00421649	0.627022	0.074583419	0.06268288	0.080896869	2.312846163	0.035305274	0.06404405	8.228990178	0.003561441	0.46122976	0.003561441	0.824324407	0.003561441	0.06718889	0.699327363	0.116527984	0.477636021	0.187306314	0.121996436	0.00320692	0.071644661	0.003662691	0.084996601	0.049966256	0.946534386	0.157306178	0.177869524	1.661517789	0.394532613	0.085649656
Citramalic acid	1.306717778	3.136403216	1.647722537	1.368509044	1.04515538	1.084728322	1.227462228	1.134416539	0.66180011	1.272748864	5.261151776	0.939292686	1.526150409	1.601174051	0.938386598	1.262129217	0.988123022	1.446524408	0.726305599	1.29455629	2.263121617	1.528490335	1.145644132	1.513800133	2.076284778	0.755845111	1.06188508	1.083374261	2.110978729	1.517079455	1.942063531	1.471705708	1.702732594	0.88044702	1.367399227	0.622036024	1.046400985	0.891704942	0.881508586	2.419171944	1.276493228	1.609256815	2.824550689	1.537528768	1.372100144	1.584833072	0.992711014	1.078774218	0.933386574	0.950201164	0.967550763	0.876088734	0.95145224	0.626858508	0.894978308	1.108789314	0.789582707	0.656635273	1.224447556	0.785866154	1.426045036	1.352825949	1.117626742	0.686007776
Gluconic acid	0.96645159	2.135064801	1.861428013	0.653836359	0.050172439	1.570800426	0.080584054	0.808417128	2.105500828	3.291060399	1.870361122	1.697125147	0.579083339	1.949417672	1.887294863	2.282401898	1.11668451	0.547774994	0.358724995	0.808792765	0.568405312	2.737347611	0.469939542	2.537247516	0.410651183	7.557440784	0.636715855	0.923931215	3.930854884	2.383707336	0.130129817	0.537291841	0.549778547	2.42441548	0.982998705	2.298482306	0.945419275	3.303553685	1.493963907	0.360563145	2.085935708	2.520509545	2.888879529	7.238675033	0.442583298	0.816230348	2.368003991	0.445103915	3.007881916	0.494314572	0.828702822	1.785848361	2.972175732	0.503165333	1.220960376	0.729840955	0.027769099	0.439262907	3.019643603	1.275160274	8.858969047	0.063175962	0.546957736	0.641629276
Glyceric acid	1.577942149	4.014289378	5.065248195	0.63137186	0.385516723	3.709645099	1.601502756	0.955510477	0.998851427	0.989294227	15.63019293	6.632471044	0.468354088	8.787135283	3.864017349	10.57285644	0.737978489	0.965128184	0.78714679	1.021932041	1.438876544	6.06995871	0.584040826	1.965658363	1.512931598	2.48650131	0.786749985	0.581081841	5.374308192	3.913272255	0.967917466	1.78778651	2.328478197	5.702193142	0.618352542	0.770731503	1.8613825	4.036375073	0.828912638	0.754587561	2.985759163	1.532669999	4.158487166	3.139362869	5.499276623	1.465039352	5.234344373	0.654365203	1.375100874	0.621385699	3.585908139	0.969904817	2.166279881	0.600306656	4.492431213	0.919782213	0.500666504	0.53186095	5.973312108	2.875056509	5.567298451	0.538364592	0.914447334	0.886760288
Hydroxyphenyllactic acid	1.500639972	2.109140184	0.820964445	0.695133353	2.377257798	0.73571578	1.495172495	1.249744861	0.653095679	1.315026587	2.170181846	1.024258734	1.448217828	1.028218866	1.07666635	0.798026155	0.994993533	0.857593616	1.36872631	0.941261323	1.480247134	1.337330586	1.018373752	1.358941295	1.11325671	1.060381814	2.167557842	1.37854589	2.224296931	1.062786958	0.952246072	1.146363547	0.796000357	0.898318348	1.301365034	2.182512136	0.630244123	0.918532047	1.494079732	0.999875262	0.938753235	1.447045276	1.887114568	1.44926409	0.864095422	1.517845185	1.814944279	0.826059202	2.640304723	1.212388763	1.277027379	1.333294312	1.215283914	0.91176926	0.765971769	0.742223899	1.390651939	1.340526016	1.005361684	0.747567769	1.641263563	0.667079844	0.914659732	0.683936111
L-3-Phenyllactic acid	1.426669253	2.507673506	1.643859953	0.697414539	2.249736038	0.624364293	1.487879354	0.706001359	0.631009634	1.284041463	2.724404403	1.321365269	0.823742645	0.652403058	0.969081259	0.773977278	0.837371726	1.001321123	0.99464016	0.88166735	1.274993392	1.659630602	0.591459835	1.476840518	0.68849068	0.788397238	6.879896602	1.001200932	1.380939592	0.671416446	0.409923595	0.833646967	1.701309533	1.517520462	1.283065965	3.05341196	0.572571337	0.789577591	1.696962851	2.016723446	0.587165381	0.927248596	1.987729989	1.137706759	1.004449113	18.03963052	2.946388074	1.075108161	1.682273529	1.132827815	1.979128436	1.903792498	0.825531268	0.875632312	0.468851005	0.48490825	1.197104726	2.321303284	1.346998087	0.600891442	1.042896306	0.886058334	0.493262486	0.725380932
Lactic acid	0.950114606	1.095543976	0.855919599	1.83200688	1.118668198	1.076953953	1.232257752	0.871697273	1.277123433	0.90430873	1.422749158	1.657121312	1.038999903	1.393953889	0.701354503	1.558209105	0.725610307	0.970894043	1.220229228	1.036446139	2.525318674	1.266429907	0.889892765	0.949977933	1.286735941	1.546122987	0.872145077	0.569535454	1.152204104	1.080384809	1.694153807	1.773149765	0.838439653	1.636311979	1.074335783	1.090380428	1.349128761	0.953490979	0.701483596	1.080799287	0.661558219	1.413575197	0.932616886	1.301600647	1.679622738	0.922568704	0.634039973	0.795282716	0.729898274	0.909926516	1.194315437	0.908965312	1.0655764	1.561962841	1.045538255	0.782661084	1.07313945	0.70373206	1.366307975	0.823201856	0.880867877	0.961159419	0.960899099	0.797222101
N-Acetylaspartic acid	0.977653423	1.535040104	1.31003855	1.17536087	0.723111752	0.796294263	1.218666502	1.008616465	1.307785774	1.091108107	0.83666598	0.808440432	0.774168238	1.049852811	1.09790248	0.955888691	1.095653359	1.241104641	1.014854629	1.623705851	0.638297409	1.190142837	1.503430231	1.28478088	0.787813104	1.336208995	1.193525484	1.178643089	1.477609698	0.983577328	0.646439771	0.801085745	1.511745616	1.21125382	1.02880447	1.23870195	0.859102017	1.02233113	1.099582929	0.726818345	1.911889714	0.809820694	1.746735536	1.646525858	1.08904671	1.12872749	1.263140066	0.751740679	1.247601715	1.844552582	0.785850688	1.368692901	1.608153342	0.914756756	1.109055518	1.17293233	0.731670126	1.020217514	1.061861678	1.515184666	1.867232327	1.377435084	1.186853994	1.483657623
N-Acetylglutamic acid	2.166189448	2.147172265	1.774566048	0.938894104	0.596139984	0.774495686	0.959546359	1.090604738	1.358347148	1.25204893	1.694814399	0.991578251	2.051491578	1.286410016	0.875979093	1.354804248	2.449504953	2.78665578	0.98314048	1.365361044	1.622128957	1.181838229	1.189924583	1.54065684	0.971191948	0.576992262	1.85273655	0.857174732	1.32233612	0.752854423	0.841777349	0.291594239	3.474827306	0.715585107	2.896507769	2.097001634	0.597424473	1.735104355	1.040755976	3.491579623	0.747092119	1.072944156	2.337200397	2.417775117	3.121842633	3.152856306	1.934824131	2.133841442	0.972243963	1.569851443	0.993951745	1.609389224	0.918487539	0.726948397	0.617069289	0.576437984	0.326282257	0.896342987	0.876697178	0.46211438	2.320953486	1.037614073	1.57737884	2.468158331
Pseudouridine	0.997710842	1.026366548	0.817966261	0.946991826	0.704609454	0.962353425	0.591165338	0.940176768	0.584890102	0.834994153	1.932679938	1.121376533	1.166348141	1.085769716	1.451337856	1.231017744	1.175245409	1.077677891	0.909221336	0.770440301	1.207305853	1.112153523	0.710259236	1.063835738	0.881464487	1.277729143	0.939631854	0.951321491	1.605869816	1.069854714	1.148833143	0.828081648	1.084884721	0.738276208	0.877229705	1.147046978	0.912600804	1.231305705	0.982928416	0.765788368	0.817152044	0.815229337	1.182588143	0.966043072	0.947967782	1.16171249	0.972757978	0.814270441	1.031022655	0.847410527	0.753635571	1.362022059	1.075437078	0.757490343	0.974060805	0.70240538	0.821520939	0.747295883	1.164905317	0.832192115	0.96791149	0.65031853	0.792871362	0.661058413
Threonic acid	0.413161079	2.785891144	3.37804427	0.978248163	0.448830562	2.083566728	0.274890992	1.105103502	1.011794152	1.649343993	4.372815867	3.21544015	0.913038356	1.308306791	2.585550028	3.391670222	1.51998792	1.099546869	0.89530568	1.715419289	0.88929384	2.419920776	1.092723548	0.537041307	0.47400646	2.296170203	1.144002609	0.783664485	1.664961413	2.696353685	0.218372605	0.836776048	1.607866724	2.524616188	1.073992723	1.40645577	1.088539204	2.700527085	1.54573432	0.873807714	2.363638411	0.623359128	2.844325173	2.909166834	0.371520422	0.474869575	4.082639394	0.940802211	1.911097229	0.801888458	2.058547328	1.477431967	1.469443854	0.726820248	2.744282962	0.754758265	0.197953613	0.722818234	2.856968367	1.65521574	3.255065393	0.662346873	1.449550366	1.317273456
MS_METABOLITE_DATA_END
#METABOLITES
METABOLITES_START
metabolite_name	quantified m/z	RT (sec)	PubChem ID	KEGG ID
2-Hydroxyglutaric acid	147.02852	93	43	C02630
3-Hydroxyisovaleric acid	117.05411	282	69362
4-Guanidinobutanoic acid	115.03852	231	500	C01035
Cholic acid	407.2803	717	221493	C00695
Citramalic acid	147.0284	132	441696	C00815
Gluconic acid	195.05023	57.6	10690	C00257
Glyceric acid	105.01772	79.8	439194	C00258
Hydroxyphenyllactic acid	181.04948	438	440177
L-3-Phenyllactic acid	165.05446	600	643327	C05607
Lactic acid	89.02274	57	107689	C00186
N-Acetylaspartic acid	174.03972	90	65065	C01042
N-Acetylglutamic acid	188.05537	120	70914	C00624
Pseudouridine	243.06186	88.2	15047	C02067
Threonic acid	135.02844	60	5460407	C01620
METABOLITES_END
#END