#METABOLOMICS WORKBENCH GeetanjaliChawla_20251119_005426 DATATRACK_ID:6723 STUDY_ID:ST004376 ANALYSIS_ID:AN007312 PROJECT_ID:PR002774 VERSION 1 CREATED_ON November 21, 2025, 2:25 pm #PROJECT PR:PROJECT_TITLE Convolvulus pluricaulis confers antidepressant and antioxidant effects through PR:PROJECT_TITLE conserved metabolic pathways in Drosophila PR:PROJECT_TYPE GC-MS PR:PROJECT_SUMMARY Convolvulus pluricaulis (Shankhpushpi) is a traditional herb used to treat PR:PROJECT_SUMMARY depression and anxiety. Using Drosophila melanogaster as a model, we identified PR:PROJECT_SUMMARY conserved metabolic and molecular pathways mediating its neuroprotective PR:PROJECT_SUMMARY effects. Metabolomic profiling of flies fed C. pluricaulis revealed altered PR:PROJECT_SUMMARY levels of ascorbic acid, glucose, and adenine monophosphate in head tissue. Gene PR:PROJECT_SUMMARY expression analysis showed significant modulation of glut1 (glucose transporter PR:PROJECT_SUMMARY 1), CG6293 (ascorbate transporter), Rdl (resistant to dieldrin), GABA-B-R1 PR:PROJECT_SUMMARY (GABA-B receptor 1), and sod1 (superoxide dismutase 1). Dietary C. pluricaulis PR:PROJECT_SUMMARY reduced depression-like behavior in a stress-induced model and elevated head PR:PROJECT_SUMMARY ascorbate levels. Knockdown of CG6293, sod1, glut1, or GABA-B-R1 abolished the PR:PROJECT_SUMMARY antioxidant effects, and knockdown of CG6293 eliminated the antidepressant PR:PROJECT_SUMMARY effects, implicating these genes as key downstream effectors. Supplementation PR:PROJECT_SUMMARY with L-ascorbic acid mimicked the behavioral and oxidative resilience conferred PR:PROJECT_SUMMARY by C. pluricaulis. Together, these findings reveal conserved antioxidant and PR:PROJECT_SUMMARY anxiolytic mechanisms underlying the pharmacological effects of C. pluricaulis PR:PROJECT_SUMMARY in Drosophila. PR:INSTITUTE Shiv Nadar Institution of Eminence, Greater Noida, India PR:DEPARTMENT Department of Life Sciences PR:LABORATORY RNA Biology Laboratory PR:LAST_NAME Chawla PR:FIRST_NAME Geetanjali PR:ADDRESS NH91, Tehsil Dadri, Greater Noida, U.P-201314, INDIA PR:EMAIL geetanjalichawla25@gmail.com PR:PHONE +91-9205007117 PR:FUNDING_SOURCE DBT-Wellcome Trust India Alliance PR:PUBLICATIONS Mitra et al., Convolvulus pluricaulis confers antidepressant and antioxidant PR:PUBLICATIONS effects through conserved metabolic and molecular pathways in Drosophila PR:CONTRIBUTORS Geetanjali Chawla #STUDY ST:STUDY_TITLE Convolvulus pluricaulis confers antidepressant and antioxidant effects through ST:STUDY_TITLE conserved metabolic and molecular pathways in Drosophila ST:STUDY_SUMMARY Convolvulus pluricaulis (Shankhpushpi) is a traditional herb used to treat ST:STUDY_SUMMARY depression and anxiety. Using Drosophila melanogaster as a model, we identified ST:STUDY_SUMMARY conserved metabolic and molecular pathways mediating its neuroprotective ST:STUDY_SUMMARY effects. Metabolomic profiling of flies fed C. pluricaulis revealed altered ST:STUDY_SUMMARY levels of ascorbic acid, glucose, and adenine monophosphate in head tissue. Gene ST:STUDY_SUMMARY expression analysis showed significant modulation of glut1 (glucose transporter ST:STUDY_SUMMARY 1), CG6293 (ascorbate transporter), Rdl (resistant to dieldrin), GABA-B-R1 ST:STUDY_SUMMARY (GABA-B receptor 1), and sod1 (superoxide dismutase 1). Dietary C. pluricaulis ST:STUDY_SUMMARY reduced depression-like behavior in a stress-induced model and elevated head ST:STUDY_SUMMARY ascorbate levels. Knockdown of CG6293, sod1, glut1, or GABA-B-R1 abolished the ST:STUDY_SUMMARY antioxidant effects, and knockdown of CG6293 eliminated the antidepressant ST:STUDY_SUMMARY effects, implicating these genes as key downstream effectors. Supplementation ST:STUDY_SUMMARY with L-ascorbic acid mimicked the behavioral and oxidative resilience conferred ST:STUDY_SUMMARY by C. pluricaulis. Together, these findings reveal conserved antioxidant and ST:STUDY_SUMMARY anxiolytic mechanisms underlying the pharmacological effects of C. pluricaulis ST:STUDY_SUMMARY in Drosophila. ST:INSTITUTE Shiv Nadar Institution of Eminence, Greater Noida, India ST:DEPARTMENT Department of Life Sciences ST:LABORATORY RNA Biology Laboratory ST:LAST_NAME Chawla ST:FIRST_NAME Geetanjali ST:ADDRESS NH91, Tehsil Dadri, Greater Noida, U.P-201314, INDIA ST:EMAIL geetanjalichawla25@gmail.com ST:PHONE +91-9205007117 ST:NUM_GROUPS 2 ST:TOTAL_SUBJECTS 200 ST:NUM_FEMALES 200 ST:PUBLICATIONS Mitra et al., Convolvulus pluricaulis confers antidepressant and antioxidant ST:PUBLICATIONS effects through conserved metabolic and molecular pathways in Drosophila #SUBJECT SU:SUBJECT_TYPE Insect SU:SUBJECT_SPECIES Drosophila melanogaster SU:TAXONOMY_ID 7227 SU:GENOTYPE_STRAIN Canton S SU:AGE_OR_AGE_RANGE 23-25 Days SU:WEIGHT_OR_WEIGHT_RANGE 0.7-1mg SU:HEIGHT_OR_HEIGHT_RANGE 1mm SU:GENDER Female SU:SPECIES_GROUP Drosophila melanogaster #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data SUBJECT_SAMPLE_FACTORS Heads_1 Et Ctrl 20d-1H Sample source:Head tissue | Treatment:Control Genotype=Canton S; RAW_FILE_NAME=Chawla_Geetanjali_2020219_5977b_5to1_DHeads_1 Et.D; RAW_FILE_NAME(RAW _FILE _NAME)=Chawla_Geetanjali_2020219_5977b_50to1_DHeads_1.D SUBJECT_SAMPLE_FACTORS Heads_2 Et Ctrl 20d-2H Sample source:Head tissue | Treatment:Control Genotype=Canton S; RAW_FILE_NAME=Chawla_Geetanjali_2020219_5977b_5to1_DHeads_2 Et.D; RAW_FILE_NAME(RAW _FILE _NAME)=Chawla_Geetanjali_2020219_5977b_50to1_DHeads_2.D SUBJECT_SAMPLE_FACTORS Heads_5 Et Ctrl 20d-3H Sample source:Head tissue | Treatment:Control Genotype=Canton S; RAW_FILE_NAME=Chawla_Geetanjali_2020219_5977b_5to1_DHeads_5 Et.D; RAW_FILE_NAME(RAW _FILE _NAME)=Chawla_Geetanjali_2020219_5977b_50to1_DHeads_5.D SUBJECT_SAMPLE_FACTORS Heads_6 Et Ctrl 20d-4H Sample source:Head tissue | Treatment:Control Genotype=Canton S; RAW_FILE_NAME=Chawla_Geetanjali_2020219_5977b_5to1_DHeads_6 Et.D; RAW_FILE_NAME(RAW _FILE _NAME)=Chawla_Geetanjali_2020219_5977b_50to1_DHeads_6.D SUBJECT_SAMPLE_FACTORS Heads_7 Sh C.p 20d-1H Sample source:Head tissue | Treatment:C. pluricaulis Genotype=Canton S; RAW_FILE_NAME=Chawla_Geetanjali_2020219_5977b_5to1_DHeads_7 Sh.D; RAW_FILE_NAME(RAW _FILE _NAME)=Chawla_Geetanjali_2020219_5977b_50to1_DHeads_7.D SUBJECT_SAMPLE_FACTORS Heads_8 Sh C.p 20d-2H Sample source:Head tissue | Treatment:C. pluricaulis Genotype=Canton S; RAW_FILE_NAME=Chawla_Geetanjali_2020219_5977b_5to1_DHeads_8 Sh.D; RAW_FILE_NAME(RAW _FILE _NAME)=Chawla_Geetanjali_2020219_5977b_50to1_DHeads_8.D SUBJECT_SAMPLE_FACTORS Heads_11 Sh C.p 20d-3H Sample source:Head tissue | Treatment:C. pluricaulis Genotype=Canton S; RAW_FILE_NAME=Chawla_Geetanjali_2020219_5977b_5to1_DHeads_11 Sh.D; RAW_FILE_NAME(RAW _FILE _NAME)=Chawla_Geetanjali_2020219_5977b_50to1_DHeads_11.D SUBJECT_SAMPLE_FACTORS Heads_12 Sh C.p 20d-4H Sample source:Head tissue | Treatment:C. pluricaulis Genotype=Canton S; RAW_FILE_NAME=Chawla_Geetanjali_2020219_5977b_5to1_DHeads_12 Sh.D; RAW_FILE_NAME(RAW _FILE _NAME)=Chawla_Geetanjali_2020219_5977b_50to1_DHeads_12.D SUBJECT_SAMPLE_FACTORS Bodies_Et2 Ctrl 20d-1B Sample source:Body tissue | Treatment:Control Genotype=Canton S; RAW_FILE_NAME=Chawla_Geetanjali_20200219_5977b_50to1_DBodies_Et2.D; RAW_FILE_NAME(RAW _FILE _NAME)=.D SUBJECT_SAMPLE_FACTORS Bodies_Et4 Ctrl 20d-2B Sample source:Body tissue | Treatment:Control Genotype=Canton S; RAW_FILE_NAME=Chawla_Geetanjali_20200219_5977b_50to1_DBodies_Et4.D; RAW_FILE_NAME(RAW _FILE _NAME)=.D SUBJECT_SAMPLE_FACTORS Bodies_Et5 Ctrl 20d-3B Sample source:Body tissue | Treatment:Control Genotype=Canton S; RAW_FILE_NAME=Chawla_Geetanjali_20200219_5977b_50to1_DBodies_Et5.D; RAW_FILE_NAME(RAW _FILE _NAME)=.D SUBJECT_SAMPLE_FACTORS Bodies_Et6 Ctrl 20d-4B Sample source:Body tissue | Treatment:Control Genotype=Canton S; RAW_FILE_NAME=Chawla_Geetanjali_20200219_5977b_50to1_DBodies_Et6.D; RAW_FILE_NAME(RAW _FILE _NAME)=Chawla_Geetanjali_20200219_5977b_150to1_DBod_Et6.D SUBJECT_SAMPLE_FACTORS Bodies_Sh2 C.p 20d-1B Sample source:Body tissue | Treatment:C. pluricaulis Genotype=Canton S; RAW_FILE_NAME=Chawla_Geetanjali_20200219_5977b_50to1_DBodies_Sh2.D; RAW_FILE_NAME(RAW _FILE _NAME)=Chawla_Geetanjali_20200219_5977b_150to1_DBod_Sh2.D SUBJECT_SAMPLE_FACTORS Bodies_Sh4 C.p 20d-2B Sample source:Body tissue | Treatment:C. pluricaulis Genotype=Canton S; RAW_FILE_NAME=Chawla_Geetanjali_20200219_5977b_50to1_DBodies_Sh4.D; RAW_FILE_NAME(RAW _FILE _NAME)=Chawla_Geetanjali_20200219_5977b_150to1_DBod_Sh4.D SUBJECT_SAMPLE_FACTORS Bodies_Sh5 C.p 20d-3B Sample source:Body tissue | Treatment:C. pluricaulis Genotype=Canton S; RAW_FILE_NAME=Chawla_Geetanjali_20200219_5977b_50to1_DBodies_Sh5.D; RAW_FILE_NAME(RAW _FILE _NAME)=Chawla_Geetanjali_20200219_5977b_150to1_DBod_Sh5.D SUBJECT_SAMPLE_FACTORS Bodies_Sh6 C.p 20d-4B Sample source:Body tissue | Treatment:C. pluricaulis Genotype=Canton S; RAW_FILE_NAME=Chawla_Geetanjali_20200219_5977b_50to1_DBodies_Sh6.D; RAW_FILE_NAME(RAW _FILE _NAME)=Chawla_Geetanjali_20200219_5977b_150to1_DBod_Sh6.D #COLLECTION CO:COLLECTION_SUMMARY Fly heads were dissected, counted and frozen in liquid nitrogen. The remaining CO:COLLECTION_SUMMARY body tissue was frozen in separate vials. The samples were frozen in liquid CO:COLLECTION_SUMMARY nitrogen in 2 mL screw cap vials with ceramic beads and stored in -80°C until CO:COLLECTION_SUMMARY preparation of the lysate. CO:SAMPLE_TYPE Head and Body tissue CO:STORAGE_CONDITIONS -80℃ #TREATMENT TR:TREATMENT_SUMMARY Canton S flies were fed a regular cornmeal diet (Vehicle) or a Convolvulus TR:TREATMENT_SUMMARY pluricaulis diet (treatment) for 20 Days. TR:TREATMENT_COMPOUND Convolvulus pluricaulis TR:TREATMENT_DOSE 100X TR:TREATMENT_VEHICLE Ethanol #SAMPLEPREP SP:SAMPLEPREP_SUMMARY The sample lysate was prepared by homogenizing the samples in 90% chilled SP:SAMPLEPREP_SUMMARY methanol, followed by one hour incubation at -20°C to precipitate proteins, and SP:SAMPLEPREP_SUMMARY the sample was centrifuged to remove insoluble debris. The supernatant was dried SP:SAMPLEPREP_SUMMARY in a SpeedVac, and the metabolite containing precipitate was analysed. #CHROMATOGRAPHY CH:CHROMATOGRAPHY_SUMMARY All GC-MS analysis was performed with an Agilent 5977b GC-MS MSD-HES and an CH:CHROMATOGRAPHY_SUMMARY Agilent 7693A automatic liquid sampler. Dried samples were suspended in 40 µL CH:CHROMATOGRAPHY_SUMMARY of a 40 mg/mL O-methoxylamine hydrochloride (MOX) (MP Bio #155405) in dry CH:CHROMATOGRAPHY_SUMMARY pyridine (EMD Millipore #PX2012-7) and incubated for one hour at 37 °C in a CH:CHROMATOGRAPHY_SUMMARY sand bath. 25 µL of this solution was added to auto sampler vials. 60 µL of CH:CHROMATOGRAPHY_SUMMARY N-methyl-N-trimethylsilyltrifluoracetamide (MSTFA with 1%TMCS, Thermo #TS48913) CH:CHROMATOGRAPHY_SUMMARY was added automatically via the auto sampler and incubated for 30 minutes at 37 CH:CHROMATOGRAPHY_SUMMARY °C. After incubation, samples were vortexed and 1 µL of the prepared sample CH:CHROMATOGRAPHY_SUMMARY was injected into the gas chromatograph inlet in the split mode with the inlet CH:CHROMATOGRAPHY_SUMMARY temperature held at 250°C. A 50:1 split ratio was used for analysis for the CH:CHROMATOGRAPHY_SUMMARY majority of metabolites. Any metabolites that saturated the instrument at the CH:CHROMATOGRAPHY_SUMMARY 50:1 split were analyzed at a 150:1 split ratio. The gas chromatograph had an CH:CHROMATOGRAPHY_SUMMARY initial temperature of 60°C for one minute followed by a 10°C/min ramp to CH:CHROMATOGRAPHY_SUMMARY 325°C and a hold time of 10 minutes. A 30-meter Agilent Zorbax DB-5MS with 10 m CH:CHROMATOGRAPHY_SUMMARY Duraguard capillary column was employed for chromatographic separation. Helium CH:CHROMATOGRAPHY_SUMMARY was used as the carrier gas at a rate of 1 mL/min. CH:CHROMATOGRAPHY_TYPE GC CH:INSTRUMENT_NAME Agilent 5977b GC-MS CH:COLUMN_NAME Agilent DB5-MS (30m x 0.25mm, 0.25um) CH:SOLVENT_A NA CH:SOLVENT_B NA CH:SOLVENT_C NA CH:FLOW_GRADIENT NA CH:FLOW_RATE NA CH:COLUMN_TEMPERATURE 60°C for one minute followed by a 10°C/min ramp to 325°C and a hold time of CH:COLUMN_TEMPERATURE 10 minutes #ANALYSIS AN:ANALYSIS_TYPE MS AN:ACQUISITION_DATE 2020-02-19 #MS MS:INSTRUMENT_NAME Agilent 5977B MS:INSTRUMENT_TYPE Single quadrupole MS:MS_TYPE EI MS:ION_MODE POSITIVE MS:MS_COMMENTS Data was collected using MassHunter software (Agilent). Metabolites were MS:MS_COMMENTS identified and their peak area was recorded using MassHunter Quant. This data MS:MS_COMMENTS was transferred to an Excel spread sheet (Microsoft, Redmond WA). Metabolite MS:MS_COMMENTS identity was established using a combination of an in-house metabolite library MS:MS_COMMENTS developed using pure purchased standards, the NIST library and the Fiehn MS:MS_COMMENTS library. There are a few reasons a specific metabolite may not be observable MS:MS_COMMENTS through GC-MS. The metabolite may not be amenable to GC-MS due to its size, or a MS:MS_COMMENTS quaternary amine such as carnitine, or simply because it does not ionize well. MS:MS_COMMENTS Metabolites that do not ionize well include oxaloacetate, histidine and MS:MS_COMMENTS arginine. Cysteine can be observed depending on cellular conditions. It often MS:MS_COMMENTS forms disulfide bonds with proteins and is generally at a low concentration. MS:MS_COMMENTS Metabolites may not be quantifiable if they are only present in very low MS:MS_COMMENTS concentrations. MS:HELIUM_FLOW 1mL/min #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS Normalized and scaled peak intensity (arbitrary units, a.u.) MS_METABOLITE_DATA_START Samples Ctrl 20d-1H Ctrl 20d-2H Ctrl 20d-3H Ctrl 20d-4H C.p 20d-1H C.p 20d-2H C.p 20d-3H C.p 20d-4H Ctrl 20d-1B Ctrl 20d-2B Ctrl 20d-3B Ctrl 20d-4B C.p 20d-1B C.p 20d-2B C.p 20d-3B C.p 20d-4B Factors Sample source:Head tissue | Treatment:Control Sample source:Head tissue | Treatment:Control Sample source:Head tissue | Treatment:Control Sample source:Head tissue | Treatment:Control Sample source:Head tissue | Treatment:C. pluricaulis Sample source:Head tissue | Treatment:C. pluricaulis Sample source:Head tissue | Treatment:C. pluricaulis Sample source:Head tissue | Treatment:C. pluricaulis Sample source:Body tissue | Treatment:Control Sample source:Body tissue | Treatment:Control Sample source:Body tissue | Treatment:Control Sample source:Body tissue | Treatment:Control Sample source:Body tissue | Treatment:C. pluricaulis Sample source:Body tissue | Treatment:C. pluricaulis Sample source:Body tissue | Treatment:C. pluricaulis Sample source:Body tissue | Treatment:C. pluricaulis 1,6-anhydro-Glucose -0.28455 0.449925 -0.05164 0.373668 -0.18433 -0.64349 0.732918 -0.39251 1-Monomyristin -1.4742 -1.49415 -1.49783 -1.51737 -1.53392 2.635318 2.770947 2.111206 -0.76027 0.149531 0.494986 0.049426 0.159639 0.028879 0.214235 -0.33643 1-Octadecene 1.798806 -1.41778 -1.33992 2.789374 -1.37727 2.413957 -1.4387 -1.42847 1-Palmitoylglycerol 0.076509 -0.38789 -1.09096 -0.69319 0.831323 0.667208 0.323457 0.273537 -0.82538 0.035116 0.059317 0.15806 0.706027 -0.1253 0.277731 -0.28557 1-Steroylglycerol -0.30471 0.15049 0.247845 -0.681 0.911128 -0.18347 0.153661 -0.29394 2-Hydroxybutyric acid 0.423715 0.632576 0.474874 0.513771 0.315274 -0.98751 -0.90949 -0.46321 0.315592 0.013023 0.743094 0.076664 -0.46212 0.223783 -0.59941 -0.31062 2-Hydroxyglutaric acid -0.17433 0.307563 0.257385 1.211059 -0.14501 -1.31009 -0.39569 0.249116 2-Ketoisocaproic acid -0.72975 -0.86925 2.534578 -0.8844 -0.81886 2.54551 -0.89528 -0.88255 2-Monopalmitoylglycerol -0.32274 -0.46415 -0.87448 -1.38499 0.598637 1.145397 0.373017 0.929308 -0.90144 0.680908 0.592346 -0.69646 0.196599 0.344041 -0.24047 0.024471 2-Steroylglycerol 2.488507 1.304037 -1.78498 1.14595 -1.82104 2.418369 -1.88036 -1.87048 3-Phosphoglyceric acid 1.403863 -1.62042 1.203735 -0.16617 0.919636 -1.44065 -1.64978 1.349769 4-Hydroxyproline 0.929654 0.737177 1.156305 0.797754 0.820121 -1.62869 -1.50802 -1.3043 -0.42092 1.065427 0.674944 0.310806 -1.20006 -0.04271 -0.80523 0.417736 Adenine 0.248796 -0.9406 -0.72839 0.059906 0.712758 -0.51924 0.587849 0.578924 Adenosine 0.45458 -0.43262 -1.00527 -0.18713 -0.24037 0.629849 1.263492 -0.48253 Adenosine monophosphate -0.56019 -0.27821 -1.14561 -0.27712 0.160571 0.661284 0.379382 1.05989 -0.43052 -0.18984 0.342695 0.707555 0.577533 -0.96723 0.253803 -0.294 Adipic acid 2.292171 -0.78667 -0.68011 -0.80333 -0.73123 2.325771 -0.8153 -0.8013 Allantoin 0.362851 0.781955 0.665908 -3.62113 0.539976 0.484186 0.671853 0.114398 alpha-D-Glucosamine 1-phosphate -0.4148 0.341094 0.569668 0.369243 0.309353 -0.90304 0.271545 -0.54306 Arachidic acid 0.440547 0.061301 -0.44381 -0.55321 -0.61663 2.147377 -0.72176 -0.31382 Arachidonic Acid -0.94753 -1.09442 2.578078 1.714712 -1.04136 -0.92661 0.82556 -1.10843 Benzoic acid 0.708218 0.161999 -0.25747 -0.93539 -0.76754 2.097507 -0.92836 -0.07898 Beta-Alanine -0.61085 0.224851 0.625849 -0.73236 0.650051 0.362596 -0.28344 -0.23669 Capric acid -0.25916 0.312227 0.767171 -0.45092 0.186401 0.562067 -0.57351 -0.54428 Cellobiose 0.319417 -0.19748 -0.17806 -0.45246 0.222769 1.05516 -0.72618 -0.04316 Cholesterol 0.798519 -1.9602 1.597692 1.308878 -1.91061 -1.80337 1.29979 0.669307 cis-Aconitic acid -0.72076 0.45594 0.947881 0.076167 0.620382 -1.21721 -0.17526 0.012859 Citric acid -0.20463 1.007341 1.018534 -0.20542 -0.15978 -0.99613 -0.09519 -0.36474 Creatinine -0.3122 2.098071 -0.38539 -0.57758 -0.46511 -0.2781 0.49471 -0.5744 Cystathionine -0.54983 0.034781 -0.4755 1.40382 0.270595 -1.16168 0.921515 -0.44371 D-Fructose -0.74422 -0.43219 -0.55372 -0.84591 -0.31467 0.8651 1.041063 0.984548 0.239341 -1.06012 -0.86274 -0.47735 0.870461 0.541716 0.260413 0.488281 D-Galactose -0.71214 -0.14499 0.654406 -0.04048 0.542278 -0.82035 0.588791 -0.06751 D-Glucose -1.45128 -1.05233 -1.11109 -1.5471 -0.86222 1.730088 1.441606 1.503135 0.514592 0.331194 -0.74782 0.107594 -0.5946 -0.58295 0.847303 0.124691 D-Malic acid 1.028067 0.977178 1.12514 0.961651 0.859975 -1.29641 -2.60685 -1.04875 -0.63745 0.47641 1.08122 -0.5411 0.244882 -0.31654 -0.23409 -0.07333 D-Mannose 0.519151 0.332696 -0.72502 0.0747 -0.59861 -0.57754 0.851136 0.123483 D-Ribose-5-phosphate -0.26261 -0.13625 -0.20197 1.268435 -1.636 1.140339 0.516922 -0.68887 D-Threitol -0.59464 -0.46047 -1.07828 0.086366 0.40727 0.371114 0.999765 0.26888 -0.10209 0.279819 -0.26097 -0.44281 0.223329 -0.04675 0.40821 -0.05874 Eicosane -1.90011 0.971582 -1.92755 -1.9996 0.894795 4.816372 -2.0066 1.15112 Elaidic acid 1.422632 1.388128 1.329469 1.200753 -3.92758 1.330722 -3.9767 1.232571 Ethanolamine -0.33804 -0.83783 0.562832 0.562369 0.736604 -1.04603 0.101665 0.258429 Ethanolamine .1 -0.05803 -0.13386 0.558075 0.371641 0.285572 -0.26237 -0.23065 -0.53038 Ethanolamine.1 -0.73029 -0.15363 -0.77749 -0.37541 -0.28842 0.616613 0.872989 0.83565 Fructose 1,6-diphosphate 1.194975 1.114364 0.896123 0.929405 1.121007 0.990795 -3.12851 -3.11816 Fructose 6-phosphate 0.065736 0.570038 -0.01242 -0.62628 -0.65213 0.162233 0.938238 -0.44542 Fumaric acid 0.929967 0.984887 1.252138 0.737105 0.629095 -1.41512 -2.21114 -0.90694 -0.17952 0.291523 0.867293 -0.45632 -0.1231 0.434795 -0.64106 -0.19361 Galactonic acid -0.88769 0.116599 -0.2221 0.476184 -0.45867 1.365158 0.242041 -0.63153 Glucose 6-phosphate -0.10287 0.384641 -0.32588 -0.00855 -0.68666 -0.10258 1.078989 -0.23709 Glutathione -0.90334 -1.02426 -0.94031 2.610619 3.22604 -0.88612 -1.04682 -1.03579 Glyceraldehyde 3-phosphate -1.81216 -1.94131 -1.85165 1.327949 2.296864 0.128969 0.275263 1.576077 Glycerol 3-phosphate -0.55144 -0.45786 0.08986 0.524413 0.989709 -0.19576 -0.19943 -0.1995 Glycine -0.14622 0.242011 0.449382 -0.821 0.365765 0.3768 -0.33645 -0.13028 Glycolic acid -0.31023 -0.67144 0.350033 0.256001 0.193603 0.426367 -0.22578 -0.01855 Guanine 0.388569 -0.22063 0.260334 -0.92731 1.652312 -1.73383 0.089392 0.491161 Heptadecanoic acid 0.440567 0.22981 -0.06662 -0.65276 -0.95454 2.052541 -0.86196 -0.18704 Heptanoic acid 0.272723 -0.09291 -0.66765 -0.07543 1.007595 -0.1817 -0.63317 0.370536 Hypoxanthine 0.459812 0.678958 0.91138 1.158981 0.728681 -0.32783 0.245823 -3.85581 Inosine 0.098989 0.272949 0.203048 0.476171 -0.26679 -1.27806 0.396134 0.097565 Inosine 5'-monophosphate -0.20065 0.557758 0.996017 0.775073 0.546518 -3.58447 0.20696 0.702792 Inositol phosphate -0.43159 -0.63995 0.192576 0.875082 0.92935 -0.68975 -0.09177 -0.14395 Isocitric acid -0.21065 0.891568 1.047893 -0.23521 -0.08244 -0.88928 -0.16572 -0.35616 L-Alanine 0.084596 -0.70518 0.335779 0.359431 0.26413 -0.27321 0.091033 -0.15657 Lanosterol -0.53249 -0.70341 2.725504 -0.72197 1.195533 -0.50815 -0.7353 -0.71971 L-Ascorbic acid -1.35336 -0.85369 -0.96108 -1.21375 -0.57339 1.825111 1.530985 1.59917 0.713454 0.723686 0.634023 0.208879 -2.36809 1.498163 -2.44643 1.036314 L-Asparagine 1.521173 0.413496 0.757544 -0.32213 -1.00587 -0.59333 -0.08101 -0.68988 -0.45634 0.094437 1.202681 0.610096 0.846418 -1.94811 -0.08433 -0.26486 L-Aspartic acid 0.989505 1.174577 1.277575 1.176563 1.005065 -1.3826 -2.39436 -1.84632 -0.69052 0.23935 0.485964 -0.96316 0.617361 0.554829 -0.31392 0.070093 Lauric Acid -1.01713 -0.89829 -0.81267 -0.71454 -0.63886 1.450033 1.496447 1.135014 -0.71258 0.942191 1.104754 0.071407 -0.09968 -0.34582 -0.801 -0.15927 L-Glutamic acid -0.71168 0.217017 1.429281 0.817411 0.599816 -1.26642 -0.64997 -0.43546 Linoleic acid -0.14553 -0.88376 -0.47682 -0.4391 -0.47716 0.827298 0.759236 0.835836 -0.21117 0.098254 0.608854 0.292083 0.061636 -1.4521 0.215095 0.387352 L-Isoleucine -0.93302 0.014902 0.445426 0.088202 0.298376 0.432756 -0.43219 0.085556 L-Lactic acid 0.236344 -0.71771 -0.73905 0.160029 0.748298 -0.05274 -0.27342 0.638254 L-Leucine -0.9818 -0.09887 0.494558 0.273864 0.37669 0.169208 -0.25454 0.02089 L-Methionine -1.03175 0.141145 0.625138 -0.35014 0.698634 0.088302 -0.22325 0.051923 L-Phenylalanine -0.85811 -0.26146 0.567536 0.496829 0.275317 0.22653 -0.41945 -0.02719 L-Phenylalanine .1 1.29168 0.313973 0.672195 0.084852 -0.15561 -1.02087 -0.62558 -0.56064 L-Proline 0.55664 0.74952 0.714363 0.771343 0.65653 -0.52297 -1.77764 -1.14778 -0.51129 0.373555 0.197291 0.710454 -0.17595 -0.89743 -0.52494 0.828311 L-Proline .1 0.790985 0.284318 0.277743 0.979471 0.205894 -1.10225 -0.76713 -0.66903 L-Serine -0.72928 0.264234 0.551324 -0.16217 0.515185 0.21474 0.032432 -0.68646 L-Serine .1 1.156283 0.038593 0.689149 0.025684 -0.83304 -0.3082 -0.34863 -0.41984 L-Sorbose -1.0023 -0.82152 -0.72931 -1.10238 -0.5509 1.565359 1.291303 1.349744 0.104637 -0.57863 -0.70543 -0.42308 0.384932 -0.4772 1.211872 0.482892 L-Threonine -0.15728 -0.92842 -0.12431 1.866272 0.568277 -1.36184 -0.73128 0.868581 L-Threonine .1 -0.07333 -0.78676 -0.71326 1.748866 0.130501 -0.17695 -0.60583 0.476768 L-Tyrosine -0.54109 0.196612 0.5026 -0.48995 0.254793 0.00933 -0.36783 0.435535 L-Valine -0.89013 0.178732 0.394146 -0.00393 0.439408 0.175588 -0.47512 0.181302 methyl-beta-D-Galactopyranoside 0.21052 -0.24336 0.21978 -0.00776 0.344258 0.973303 -0.86848 -0.62826 Methylmalonic acid -0.32841 -1.07807 0.439218 0.274391 0.30616 0.012661 -0.02214 0.396189 Myoinositol -0.00621 -0.04716 -0.07076 0.056004 -0.01269 0.978184 -0.32679 -0.57058 Myristic acid -0.10635 -1.13235 -0.8106 -0.78441 0.075597 1.138611 1.11442 0.505087 -0.38077 0.68349 0.795805 0.203721 -0.27056 -0.50265 -0.42531 -0.10372 N-acetyl-L-aspartic acid -1.03236 1.554184 -1.08172 -0.2236 -1.13548 2.038467 -1.22391 1.10442 N-acetyl-Lysine -1.02337 0.379434 0.519092 -0.00516 0.499689 0.103929 -0.52224 0.048631 Nicotinamide -1.08375 -0.05767 0.36267 0.30064 0.431216 0.476373 -0.30322 -0.12626 Nitrous Oxide 0.094111 0.10814 -0.09174 -0.47159 0.572496 0.419689 -0.7342 0.103092 Nonanoic acid -0.3198 0.402309 0.728002 -0.50081 0.817871 -0.07078 -0.6931 -0.36369 Oleic acid 0.032058 0.27451 0.590192 -0.13238 0.037042 -1.16 0.038827 0.319746 O-Phosphoethanolamine -0.53743 -0.54661 0.368853 0.32508 1.143745 -0.3136 -0.26351 -0.17653 Ornithine -0.65456 0.391678 0.928291 -0.25398 0.770614 -0.70394 -0.24552 -0.23259 Oxalic acid -0.36998 -0.35604 0.504304 -0.26667 0.578436 0.061262 -0.48212 0.330814 Palmitic acid 0.316324 -0.49095 0.549933 -0.08979 -0.18242 0.426172 0.037893 -0.56716 Palmitoleic acid -0.64043 -0.83645 -0.78809 -0.72168 -0.41952 1.139354 1.449941 0.816864 -0.13039 0.504812 0.66788 0.124896 -0.54269 -0.86528 -0.18775 0.428518 Phosphate 0.341491 -0.15714 0.453369 -0.39299 0.294266 0.341455 -0.43805 -0.4424 Putrescine -2.6662 0.2032 1.322263 1.251017 0.925481 -2.6476 0.717611 0.894224 Pyroglutamic acid -0.34226 0.318886 1.095692 -0.32105 0.403004 -0.1961 -0.43825 -0.51993 Pyrophosphate 0.29748 0.611131 0.848125 0.62423 0.592856 -4.1653 0.473424 0.718052 Pyrrolidonecarboxylic acide hit 0.048961 0.314179 0.542359 -0.62944 -0.14524 1.074893 -0.19981 -1.0059 Pyruvic acid -0.98525 -0.99132 -0.8666 -0.67155 -0.80126 1.643872 2.350468 0.321633 0.264571 0.657147 0.326121 -0.27039 -0.56852 -0.29863 0.191161 -0.30146 Ribitol -0.83793 -0.35336 0.446393 -0.002 0.615609 0.358251 -0.25582 0.028855 Ribonic acid-gamma-lactone 0.632977 -1.23806 0.626921 0.162905 0.694367 -0.99306 1.372456 -1.25851 Sedoheptulose 7-phosphate -0.19088 0.264069 -0.36788 -0.44897 0.123807 0.339473 0.477607 -0.19722 Sophorose 1.01011 1.423121 0.846862 0.467783 1.498522 -3.36556 1.610743 -3.49158 Sorbitol -1.00333 -0.41667 -0.78762 -0.4998 -0.02314 0.913753 0.390537 1.426273 -0.41633 -0.04454 -0.26286 -0.1455 0.643716 0.33718 0.351439 -0.4631 Stearic acid 0.24865 -0.4942 0.246619 -0.20069 -0.07347 1.084326 -0.17014 -0.64109 Succinic acid 0.735243 0.98109 0.694985 0.831747 0.815946 -1.18844 -2.11476 -0.75581 -0.39714 -0.58096 -0.07633 0.478616 0.846156 0.526409 -0.92603 0.129278 Sucrose 0.430144 0.362837 0.161857 0.862163 0.760525 0.963822 -4.01543 0.474087 Taurine 0.394301 1.069976 0.826937 0.53106 -0.711 -1.00036 -0.66123 -0.44969 0.060799 0.377399 0.765953 0.7582 1.218066 -3.43805 -0.04066 0.29829 Threonic acid 0.911337 1.102957 1.083191 0.942718 0.888839 -2.20024 -2.42674 -0.30206 -0.06723 -0.0031 0.313062 0.338219 0.585873 0.048799 -1.32342 0.107789 Thymine -3.37809 -0.50177 0.673672 0.628642 0.820465 1.200481 0.2053 0.351299 Turanose -1.25484 -1.34518 -1.28246 4.440858 -1.31255 -1.24197 3.349938 -1.3538 Uracil -0.82988 -0.4673 -0.77202 -0.70737 -0.39508 1.235732 1.249602 0.686312 -0.10086 -0.38198 -0.62381 1.281351 0.189602 -0.35462 -0.0951 0.085419 Urea -0.46333 -0.62675 -0.24846 0.728724 0.807121 -0.9829 -0.14402 0.929619 Xanthurenic acid -0.36771 0.431151 0.622802 -1.08389 0.4043 0.125392 0.546908 -0.67895 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name 1,6-anhydro-Glucose 1-Monomyristin 1-Octadecene 1-Palmitoylglycerol 1-Steroylglycerol 2-Hydroxybutyric acid 2-Hydroxyglutaric acid 2-Ketoisocaproic acid 2-Monopalmitoylglycerol 2-Steroylglycerol 3-Phosphoglyceric acid 4-Hydroxyproline Adenine Adenosine Adenosine monophosphate Adipic acid Allantoin alpha-D-Glucosamine 1-phosphate Arachidic acid Arachidonic Acid Benzoic acid Beta-Alanine Capric acid Cellobiose Cholesterol cis-Aconitic acid Citric acid Creatinine Cystathionine D-Fructose D-Galactose D-Glucose D-Malic acid D-Mannose D-Ribose-5-phosphate D-Threitol Eicosane Elaidic acid Ethanolamine Ethanolamine .1 Ethanolamine.1 Fructose 1,6-diphosphate Fructose 6-phosphate Fumaric acid Galactonic acid Glucose 6-phosphate Glutathione Glyceraldehyde 3-phosphate Glycerol 3-phosphate Glycine Glycolic acid Guanine Heptadecanoic acid Heptanoic acid Hypoxanthine Inosine Inosine 5'-monophosphate Inositol phosphate Isocitric acid L-Alanine Lanosterol L-Ascorbic acid L-Asparagine L-Aspartic acid Lauric Acid L-Glutamic acid Linoleic acid L-Isoleucine L-Lactic acid L-Leucine L-Methionine L-Phenylalanine L-Phenylalanine .1 L-Proline L-Proline .1 L-Serine L-Serine .1 L-Sorbose L-Threonine L-Threonine .1 L-Tyrosine L-Valine methyl-beta-D-Galactopyranoside Methylmalonic acid Myoinositol Myristic acid N-acetyl-L-aspartic acid N-acetyl-Lysine Nicotinamide Nitrous Oxide Nonanoic acid Oleic acid O-Phosphoethanolamine Ornithine Oxalic acid Palmitic acid Palmitoleic acid Phosphate Putrescine Pyroglutamic acid Pyrophosphate Pyrrolidonecarboxylic acide hit Pyruvic acid Ribitol Ribonic acid-gamma-lactone Sedoheptulose 7-phosphate Sophorose Sorbitol Stearic acid Succinic acid Sucrose Taurine Threonic acid Thymine Turanose Uracil Urea Xanthurenic acid METABOLITES_END #END