#METABOLOMICS WORKBENCH lizhipeng_20251117_022202 DATATRACK_ID:6706 STUDY_ID:ST004383 ANALYSIS_ID:AN007323 PROJECT_ID:PR002777
VERSION                          	1
CREATED_ON                       	11-24-2025
#PROJECT
PR:PROJECT_TITLE                 	Microbiome biogeography and ventral epithelial differences of rumen across
PR:PROJECT_TITLE                 	different regions in three different ruminant species
PR:PROJECT_SUMMARY               	Ruminants thrive in diverse ecosystems by leveraging their rumen microbiome to
PR:PROJECT_SUMMARY               	ferment fibrous plants. However, the spatial biogeography of the rumen
PR:PROJECT_SUMMARY               	microbiome and genetic diversity of ventral rumen among the ruminants remain
PR:PROJECT_SUMMARY               	unknown. Here, we present a multi-omics integrating region-resolved microbiome
PR:PROJECT_SUMMARY               	across eleven ruminal sacs, metabolomics, and single-cell RNA sequencing,
PR:PROJECT_SUMMARY               	ATAC-seq, and bulk RNA-seq of ventral epithelium of roe deer, sika deer and
PR:PROJECT_SUMMARY               	sheep. Our results reveal species-specific rumen microbial compositions and
PR:PROJECT_SUMMARY               	metabolic capacities that contribute to differences in short-chain fatty acids
PR:PROJECT_SUMMARY               	and vitamin B production. We uncover functional divergence, genomic
PR:PROJECT_SUMMARY               	specialization and metabolic changes across distinct ruminal sacs microbiome.
PR:PROJECT_SUMMARY               	Single-cell profiling reveals changes of immune responses and structural
PR:PROJECT_SUMMARY               	remodeling in ventral rumen. We demonstrate that vitamin B12 promotes epithelial
PR:PROJECT_SUMMARY               	growth, and four novel genes that enhance stem cell differentiation. Our results
PR:PROJECT_SUMMARY               	highlight variation in microbial ecology and epithelial architecture among three
PR:PROJECT_SUMMARY               	ruminant species, offering insights to improve livestock productivity.
PR:INSTITUTE                     	Jilin Agricultural University
PR:DEPARTMENT                    	College of Animal Science and Technology
PR:LABORATORY                    	Ruminant Nutrition Laboratory
PR:LAST_NAME                     	Li
PR:FIRST_NAME                    	Zhi Peng
PR:ADDRESS                       	No. 2888 Xincheng Street
PR:EMAIL                         	zhplicaas@163.com
PR:PHONE                         	18043213681
PR:DOI                           	http://dx.doi.org/10.21228/M8Q26C
#STUDY
ST:STUDY_TITLE                   	GC-FID based metabolomic profiling of rumen samples across different regions in
ST:STUDY_TITLE                   	three ruminant species - investigation of short-chain fatty acids
ST:STUDY_TYPE                    	GC-FID metabolomics
ST:STUDY_SUMMARY                 	This study investigates short-chain fatty acids (SCFAs) in the rumen of three
ST:STUDY_SUMMARY                 	ruminant species. Rumen contents and rumen fluid were collected from multiple
ST:STUDY_SUMMARY                 	anatomical regions, and SCFA concentrations were quantified using GC-FID. The
ST:STUDY_SUMMARY                 	analysis revealed species-specific fermentation patterns, with roe deer
ST:STUDY_SUMMARY                 	exhibiting elevated acetate levels, sika deer showing increased propionate, and
ST:STUDY_SUMMARY                 	sheep displaying higher concentrations of isobutyrate and isovalerate.
ST:INSTITUTE                     	Jilin Agricultural University
ST:DEPARTMENT                    	College of Animal Science and Technology
ST:LABORATORY                    	Ruminant Nutrition Laboratory
ST:LAST_NAME                     	Li
ST:FIRST_NAME                    	Zhi Peng
ST:ADDRESS                       	No. 2888 Xincheng Street
ST:EMAIL                         	zhplicaas@163.com
ST:PHONE                         	18043213681
ST:SUBMIT_DATE                   	2025-11-17
#SUBJECT
SU:SUBJECT_TYPE                  	Mammal
SU:SUBJECT_SPECIES               	Ovis aries, Capreolus pygargus, Cervus nippon
SU:TAXONOMY_ID                   	9940, 48560, 9863
SU:AGE_OR_AGE_RANGE              	4 years
SU:GENDER                        	Male
SU:ANIMAL_HOUSING                	Each animal was housed in an individual pen (3 m × 3 m).
SU:ANIMAL_FEED                   	Roe deer, sika deer and sheep fed a total mixed ration consisting of alfalfa and
SU:ANIMAL_FEED                   	concentrate (45:55, dry matter basis) for two months were used in this study.
SU:ANIMAL_WATER                  	Free water
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data
SUBJECT_SAMPLE_FACTORS           	RD_A	108_FIDIA	Sample source:rumen content | Regions:AR | Host:Capreolus pygargus	RAW_FILE_NAME(Raw file name)=108_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	RD_A	119_FIDIA	Sample source:rumen content | Regions:AR | Host:Capreolus pygargus	RAW_FILE_NAME(Raw file name)=119_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	RD_A	120_FIDIA	Sample source:rumen content | Regions:AR | Host:Capreolus pygargus	RAW_FILE_NAME(Raw file name)=120_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	RD_A	163_FIDIA	Sample source:rumen content | Regions:AR | Host:Capreolus pygargus	RAW_FILE_NAME(Raw file name)=163_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SD_B	06_FIDIA	Sample source:rumen content | Regions:AR | Host:Cervus nippon	RAW_FILE_NAME(Raw file name)=06_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SD_B	101_FIDIA	Sample source:rumen content | Regions:AR | Host:Cervus nippon	RAW_FILE_NAME(Raw file name)=101_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SD_B	125_FIDIA	Sample source:rumen content | Regions:AR | Host:Cervus nippon	RAW_FILE_NAME(Raw file name)=125_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SD_B	12_FIDIA	Sample source:rumen content | Regions:AR | Host:Cervus nippon	RAW_FILE_NAME(Raw file name)=12_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SD_B	143_FIDIA	Sample source:rumen content | Regions:AR | Host:Cervus nippon	RAW_FILE_NAME(Raw file name)=143_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SH_C	115_FIDIA	Sample source:rumen content | Regions:AR | Host:Ovis aries	RAW_FILE_NAME(Raw file name)=115_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SH_C	26_FIDIA	Sample source:rumen content | Regions:AR | Host:Ovis aries	RAW_FILE_NAME(Raw file name)=26_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SH_C	27_FIDIA	Sample source:rumen content | Regions:AR | Host:Ovis aries	RAW_FILE_NAME(Raw file name)=27_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SH_C	72_FIDIA	Sample source:rumen content | Regions:AR | Host:Ovis aries	RAW_FILE_NAME(Raw file name)=72_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SH_C	99_FIDIA	Sample source:rumen content | Regions:AR | Host:Ovis aries	RAW_FILE_NAME(Raw file name)=99_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	RD_A	112_FIDIA	Sample source:rumen content | Regions:DSR | Host:Capreolus pygargus	RAW_FILE_NAME(Raw file name)=112_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	RD_A	130_FIDIA	Sample source:rumen content | Regions:DSR | Host:Capreolus pygargus	RAW_FILE_NAME(Raw file name)=130_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	RD_A	66_FIDIA	Sample source:rumen content | Regions:DSR | Host:Capreolus pygargus	RAW_FILE_NAME(Raw file name)=66_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	RD_A	76_FIDIA	Sample source:rumen content | Regions:DSR | Host:Capreolus pygargus	RAW_FILE_NAME(Raw file name)=76_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	RD_A	93_FIDIA	Sample source:rumen content | Regions:DSR | Host:Capreolus pygargus	RAW_FILE_NAME(Raw file name)=93_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SD_B	02_FIDIA	Sample source:rumen content | Regions:DSR | Host:Cervus nippon	RAW_FILE_NAME(Raw file name)=02_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SD_B	109_FIDIA	Sample source:rumen content | Regions:DSR | Host:Cervus nippon	RAW_FILE_NAME(Raw file name)=109_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SD_B	16_FIDIA	Sample source:rumen content | Regions:DSR | Host:Cervus nippon	RAW_FILE_NAME(Raw file name)=16_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SD_B	21_FIDIA	Sample source:rumen content | Regions:DSR | Host:Cervus nippon	RAW_FILE_NAME(Raw file name)=21_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SD_B	33_FIDIA	Sample source:rumen content | Regions:DSR | Host:Cervus nippon	RAW_FILE_NAME(Raw file name)=33_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SH_C	105_FIDIA	Sample source:rumen content | Regions:DSR | Host:Ovis aries	RAW_FILE_NAME(Raw file name)=105_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SH_C	135_FIDIA	Sample source:rumen content | Regions:DSR | Host:Ovis aries	RAW_FILE_NAME(Raw file name)=135_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SH_C	15_FIDIA	Sample source:rumen content | Regions:DSR | Host:Ovis aries	RAW_FILE_NAME(Raw file name)=15_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SH_C	28_FIDIA	Sample source:rumen content | Regions:DSR | Host:Ovis aries	RAW_FILE_NAME(Raw file name)=28_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SH_C	65_FIDIA	Sample source:rumen content | Regions:DSR | Host:Ovis aries	RAW_FILE_NAME(Raw file name)=65_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	RD_A	136_FIDIA	Sample source:rumen content | Regions:IR | Host:Capreolus pygargus	RAW_FILE_NAME(Raw file name)=136_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	RD_A	156_FIDIA	Sample source:rumen content | Regions:IR | Host:Capreolus pygargus	RAW_FILE_NAME(Raw file name)=156_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	RD_A	52_FIDIA	Sample source:rumen content | Regions:IR | Host:Capreolus pygargus	RAW_FILE_NAME(Raw file name)=52_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	RD_A	63_FIDIA	Sample source:rumen content | Regions:IR | Host:Capreolus pygargus	RAW_FILE_NAME(Raw file name)=63_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	RD_A	80_FIDIA	Sample source:rumen content | Regions:IR | Host:Capreolus pygargus	RAW_FILE_NAME(Raw file name)=80_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SD_B	139_FIDIA	Sample source:rumen content | Regions:IR | Host:Cervus nippon	RAW_FILE_NAME(Raw file name)=139_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SD_B	153_FIDIA	Sample source:rumen content | Regions:IR | Host:Cervus nippon	RAW_FILE_NAME(Raw file name)=153_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SD_B	23_FIDIA	Sample source:rumen content | Regions:IR | Host:Cervus nippon	RAW_FILE_NAME(Raw file name)=23_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SD_B	25_FIDIA	Sample source:rumen content | Regions:IR | Host:Cervus nippon	RAW_FILE_NAME(Raw file name)=25_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SD_B	68_FIDIA	Sample source:rumen content | Regions:IR | Host:Cervus nippon	RAW_FILE_NAME(Raw file name)=68_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SH_C	141_FIDIA	Sample source:rumen content | Regions:IR | Host:Ovis aries	RAW_FILE_NAME(Raw file name)=141_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SH_C	149_FIDIA	Sample source:rumen content | Regions:IR | Host:Ovis aries	RAW_FILE_NAME(Raw file name)=149_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SH_C	30_FIDIA	Sample source:rumen content | Regions:IR | Host:Ovis aries	RAW_FILE_NAME(Raw file name)=30_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SH_C	31_FIDIA	Sample source:rumen content | Regions:IR | Host:Ovis aries	RAW_FILE_NAME(Raw file name)=31_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SH_C	56_FIDIA	Sample source:rumen content | Regions:IR | Host:Ovis aries	RAW_FILE_NAME(Raw file name)=56_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	RD_A	129_FIDIA	Sample source:rumen content | Regions:SAR | Host:Capreolus pygargus	RAW_FILE_NAME(Raw file name)=129_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	RD_A	154_FIDIA	Sample source:rumen content | Regions:SAR | Host:Capreolus pygargus	RAW_FILE_NAME(Raw file name)=154_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	RD_A	45_FIDIA	Sample source:rumen content | Regions:SAR | Host:Capreolus pygargus	RAW_FILE_NAME(Raw file name)=45_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	RD_A	78_FIDIA	Sample source:rumen content | Regions:SAR | Host:Capreolus pygargus	RAW_FILE_NAME(Raw file name)=78_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	RD_A	88_FIDIA	Sample source:rumen content | Regions:SAR | Host:Capreolus pygargus	RAW_FILE_NAME(Raw file name)=88_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SD_B	122_FIDIA	Sample source:rumen content | Regions:SAR | Host:Cervus nippon	RAW_FILE_NAME(Raw file name)=122_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SD_B	132_FIDIA	Sample source:rumen content | Regions:SAR | Host:Cervus nippon	RAW_FILE_NAME(Raw file name)=132_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SD_B	53_FIDIA	Sample source:rumen content | Regions:SAR | Host:Cervus nippon	RAW_FILE_NAME(Raw file name)=53_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SD_B	58_FIDIA	Sample source:rumen content | Regions:SAR | Host:Cervus nippon	RAW_FILE_NAME(Raw file name)=58_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SD_B	75_FIDIA	Sample source:rumen content | Regions:SAR | Host:Cervus nippon	RAW_FILE_NAME(Raw file name)=75_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SH_C	10_FIDIA	Sample source:rumen content | Regions:SAR | Host:Ovis aries	RAW_FILE_NAME(Raw file name)=10_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SH_C	117_FIDIA	Sample source:rumen content | Regions:SAR | Host:Ovis aries	RAW_FILE_NAME(Raw file name)=117_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SH_C	137_FIDIA	Sample source:rumen content | Regions:SAR | Host:Ovis aries	RAW_FILE_NAME(Raw file name)=137_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SH_C	19_FIDIA	Sample source:rumen content | Regions:SAR | Host:Ovis aries	RAW_FILE_NAME(Raw file name)=19_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SH_C	22_FIDIA	Sample source:rumen content | Regions:SAR | Host:Ovis aries	RAW_FILE_NAME(Raw file name)=22_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	RD_A	04_FIDIA	Sample source:rumen content | Regions:SASR | Host:Capreolus pygargus	RAW_FILE_NAME(Raw file name)=04_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	RD_A	102_FIDIA	Sample source:rumen content | Regions:SASR | Host:Capreolus pygargus	RAW_FILE_NAME(Raw file name)=102_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	RD_A	17_FIDIA	Sample source:rumen content | Regions:SASR | Host:Capreolus pygargus	RAW_FILE_NAME(Raw file name)=17_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	RD_A	35_FIDIA	Sample source:rumen content | Regions:SASR | Host:Capreolus pygargus	RAW_FILE_NAME(Raw file name)=35_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	RD_A	85_FIDIA	Sample source:rumen content | Regions:SASR | Host:Capreolus pygargus	RAW_FILE_NAME(Raw file name)=85_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SD_B	110_FIDIA	Sample source:rumen content | Regions:SASR | Host:Cervus nippon	RAW_FILE_NAME(Raw file name)=110_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SD_B	131_FIDIA	Sample source:rumen content | Regions:SASR | Host:Cervus nippon	RAW_FILE_NAME(Raw file name)=131_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SD_B	161_FIDIA	Sample source:rumen content | Regions:SASR | Host:Cervus nippon	RAW_FILE_NAME(Raw file name)=161_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SD_B	39_FIDIA	Sample source:rumen content | Regions:SASR | Host:Cervus nippon	RAW_FILE_NAME(Raw file name)=39_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SD_B	84_FIDIA	Sample source:rumen content | Regions:SASR | Host:Cervus nippon	RAW_FILE_NAME(Raw file name)=84_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SH_C	100_FIDIA	Sample source:rumen content | Regions:SASR | Host:Ovis aries	RAW_FILE_NAME(Raw file name)=100_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SH_C	134_FIDIA	Sample source:rumen content | Regions:SASR | Host:Ovis aries	RAW_FILE_NAME(Raw file name)=134_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SH_C	144_FIDIA	Sample source:rumen content | Regions:SASR | Host:Ovis aries	RAW_FILE_NAME(Raw file name)=144_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SH_C	51_FIDIA	Sample source:rumen content | Regions:SASR | Host:Ovis aries	RAW_FILE_NAME(Raw file name)=51_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SH_C	98_FIDIA	Sample source:rumen content | Regions:SASR | Host:Ovis aries	RAW_FILE_NAME(Raw file name)=98_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	RD_A	147_FIDIA	Sample source:rumen content | Regions:SCR | Host:Capreolus pygargus	RAW_FILE_NAME(Raw file name)=147_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	RD_A	57_FIDIA	Sample source:rumen content | Regions:SCR | Host:Capreolus pygargus	RAW_FILE_NAME(Raw file name)=57_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	RD_A	81_FIDIA	Sample source:rumen content | Regions:SCR | Host:Capreolus pygargus	RAW_FILE_NAME(Raw file name)=81_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	RD_A	83_FIDIA	Sample source:rumen content | Regions:SCR | Host:Capreolus pygargus	RAW_FILE_NAME(Raw file name)=83_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	RD_A	91_FIDIA	Sample source:rumen content | Regions:SCR | Host:Capreolus pygargus	RAW_FILE_NAME(Raw file name)=91_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SD_B	126_FIDIA	Sample source:rumen content | Regions:SCR | Host:Cervus nippon	RAW_FILE_NAME(Raw file name)=126_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SD_B	151_FIDIA	Sample source:rumen content | Regions:SCR | Host:Cervus nippon	RAW_FILE_NAME(Raw file name)=151_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SD_B	41_FIDIA	Sample source:rumen content | Regions:SCR | Host:Cervus nippon	RAW_FILE_NAME(Raw file name)=41_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SD_B	47_FIDIA	Sample source:rumen content | Regions:SCR | Host:Cervus nippon	RAW_FILE_NAME(Raw file name)=47_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SD_B	62_FIDIA	Sample source:rumen content | Regions:SCR | Host:Cervus nippon	RAW_FILE_NAME(Raw file name)=62_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SH_C	03_FIDIA	Sample source:rumen content | Regions:SCR | Host:Ovis aries	RAW_FILE_NAME(Raw file name)=03_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SH_C	05_FIDIA	Sample source:rumen content | Regions:SCR | Host:Ovis aries	RAW_FILE_NAME(Raw file name)=05_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SH_C	127_FIDIA	Sample source:rumen content | Regions:SCR | Host:Ovis aries	RAW_FILE_NAME(Raw file name)=127_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SH_C	145_FIDIA	Sample source:rumen content | Regions:SCR | Host:Ovis aries	RAW_FILE_NAME(Raw file name)=145_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SH_C	69_FIDIA	Sample source:rumen content | Regions:SCR | Host:Ovis aries	RAW_FILE_NAME(Raw file name)=69_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	RD_A	116_FIDIA	Sample source:rumen content | Regions:SCVR | Host:Capreolus pygargus	RAW_FILE_NAME(Raw file name)=116_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	RD_A	18_FIDIA	Sample source:rumen content | Regions:SCVR | Host:Capreolus pygargus	RAW_FILE_NAME(Raw file name)=18_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	RD_A	49_FIDIA	Sample source:rumen content | Regions:SCVR | Host:Capreolus pygargus	RAW_FILE_NAME(Raw file name)=49_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	RD_A	95_FIDIA	Sample source:rumen content | Regions:SCVR | Host:Capreolus pygargus	RAW_FILE_NAME(Raw file name)=95_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SD_B	106_FIDIA	Sample source:rumen content | Regions:SCVR | Host:Cervus nippon	RAW_FILE_NAME(Raw file name)=106_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SD_B	114_FIDIA	Sample source:rumen content | Regions:SCVR | Host:Cervus nippon	RAW_FILE_NAME(Raw file name)=114_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SD_B	140_FIDIA	Sample source:rumen content | Regions:SCVR | Host:Cervus nippon	RAW_FILE_NAME(Raw file name)=140_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SD_B	142_FIDIA	Sample source:rumen content | Regions:SCVR | Host:Cervus nippon	RAW_FILE_NAME(Raw file name)=142_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SD_B	61_FIDIA	Sample source:rumen content | Regions:SCVR | Host:Cervus nippon	RAW_FILE_NAME(Raw file name)=61_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SH_C	160_FIDIA	Sample source:rumen content | Regions:SCVR | Host:Ovis aries	RAW_FILE_NAME(Raw file name)=160_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SH_C	20_FIDIA	Sample source:rumen content | Regions:SCVR | Host:Ovis aries	RAW_FILE_NAME(Raw file name)=20_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SH_C	29_FIDIA	Sample source:rumen content | Regions:SCVR | Host:Ovis aries	RAW_FILE_NAME(Raw file name)=29_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SH_C	43_FIDIA	Sample source:rumen content | Regions:SCVR | Host:Ovis aries	RAW_FILE_NAME(Raw file name)=43_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SH_C	60_FIDIA	Sample source:rumen content | Regions:SCVR | Host:Ovis aries	RAW_FILE_NAME(Raw file name)=60_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	RD_A	107_FIDIA	Sample source:rumen content | Regions:SDR | Host:Capreolus pygargus	RAW_FILE_NAME(Raw file name)=107_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	RD_A	124_FIDIA	Sample source:rumen content | Regions:SDR | Host:Capreolus pygargus	RAW_FILE_NAME(Raw file name)=124_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	RD_A	14_FIDIA	Sample source:rumen content | Regions:SDR | Host:Capreolus pygargus	RAW_FILE_NAME(Raw file name)=14_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	RD_A	152_FIDIA	Sample source:rumen content | Regions:SDR | Host:Capreolus pygargus	RAW_FILE_NAME(Raw file name)=152_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	RD_A	54_FIDIA	Sample source:rumen content | Regions:SDR | Host:Capreolus pygargus	RAW_FILE_NAME(Raw file name)=54_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SD_B	08_FIDIA	Sample source:rumen content | Regions:SDR | Host:Cervus nippon	RAW_FILE_NAME(Raw file name)=08_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SD_B	121_FIDIA	Sample source:rumen content | Regions:SDR | Host:Cervus nippon	RAW_FILE_NAME(Raw file name)=121_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SD_B	13_FIDIA	Sample source:rumen content | Regions:SDR | Host:Cervus nippon	RAW_FILE_NAME(Raw file name)=13_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SD_B	50_FIDIA	Sample source:rumen content | Regions:SDR | Host:Cervus nippon	RAW_FILE_NAME(Raw file name)=50_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SD_B	59_FIDIA	Sample source:rumen content | Regions:SDR | Host:Cervus nippon	RAW_FILE_NAME(Raw file name)=59_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SH_C	123_FIDIA	Sample source:rumen content | Regions:SDR | Host:Ovis aries	RAW_FILE_NAME(Raw file name)=123_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SH_C	128_FIDIA	Sample source:rumen content | Regions:SDR | Host:Ovis aries	RAW_FILE_NAME(Raw file name)=128_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SH_C	133_FIDIA	Sample source:rumen content | Regions:SDR | Host:Ovis aries	RAW_FILE_NAME(Raw file name)=133_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SH_C	40_FIDIA	Sample source:rumen content | Regions:SDR | Host:Ovis aries	RAW_FILE_NAME(Raw file name)=40_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SH_C	44_FIDIA	Sample source:rumen content | Regions:SDR | Host:Ovis aries	RAW_FILE_NAME(Raw file name)=44_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	RD_A	157_FIDIA	Sample source:rumen content | Regions:SLSR | Host:Capreolus pygargus	RAW_FILE_NAME(Raw file name)=157_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	RD_A	55_FIDIA	Sample source:rumen content | Regions:SLSR | Host:Capreolus pygargus	RAW_FILE_NAME(Raw file name)=55_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	RD_A	67_FIDIA	Sample source:rumen content | Regions:SLSR | Host:Capreolus pygargus	RAW_FILE_NAME(Raw file name)=67_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	RD_A	94_FIDIA	Sample source:rumen content | Regions:SLSR | Host:Capreolus pygargus	RAW_FILE_NAME(Raw file name)=94_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	RD_A	96_FIDIA	Sample source:rumen content | Regions:SLSR | Host:Capreolus pygargus	RAW_FILE_NAME(Raw file name)=96_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SD_B	34_FIDIA	Sample source:rumen content | Regions:SLSR | Host:Cervus nippon	RAW_FILE_NAME(Raw file name)=34_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SD_B	36_FIDIA	Sample source:rumen content | Regions:SLSR | Host:Cervus nippon	RAW_FILE_NAME(Raw file name)=36_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SD_B	37_FIDIA	Sample source:rumen content | Regions:SLSR | Host:Cervus nippon	RAW_FILE_NAME(Raw file name)=37_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SD_B	86_FIDIA	Sample source:rumen content | Regions:SLSR | Host:Cervus nippon	RAW_FILE_NAME(Raw file name)=86_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SD_B	87_FIDIA	Sample source:rumen content | Regions:SLSR | Host:Cervus nippon	RAW_FILE_NAME(Raw file name)=87_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SH_C	01_FIDIA	Sample source:rumen content | Regions:SLSR | Host:Ovis aries	RAW_FILE_NAME(Raw file name)=01_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SH_C	158_FIDIA	Sample source:rumen content | Regions:SLSR | Host:Ovis aries	RAW_FILE_NAME(Raw file name)=158_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SH_C	32_FIDIA	Sample source:rumen content | Regions:SLSR | Host:Ovis aries	RAW_FILE_NAME(Raw file name)=32_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SH_C	46_FIDIA	Sample source:rumen content | Regions:SLSR | Host:Ovis aries	RAW_FILE_NAME(Raw file name)=46_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SH_C	71_FIDIA	Sample source:rumen content | Regions:SLSR | Host:Ovis aries	RAW_FILE_NAME(Raw file name)=71_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	RD_A	148_FIDIA	Sample source:rumen content | Regions:SVR | Host:Capreolus pygargus	RAW_FILE_NAME(Raw file name)=148_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	RD_A	150_FIDIA	Sample source:rumen content | Regions:SVR | Host:Capreolus pygargus	RAW_FILE_NAME(Raw file name)=150_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	RD_A	155_FIDIA	Sample source:rumen content | Regions:SVR | Host:Capreolus pygargus	RAW_FILE_NAME(Raw file name)=155_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	RD_A	64_FIDIA	Sample source:rumen content | Regions:SVR | Host:Capreolus pygargus	RAW_FILE_NAME(Raw file name)=64_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	RD_A	79_FIDIA	Sample source:rumen content | Regions:SVR | Host:Capreolus pygargus	RAW_FILE_NAME(Raw file name)=79_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SD_B	103_FIDIA	Sample source:rumen content | Regions:SVR | Host:Cervus nippon	RAW_FILE_NAME(Raw file name)=103_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SD_B	11_FIDIA	Sample source:rumen content | Regions:SVR | Host:Cervus nippon	RAW_FILE_NAME(Raw file name)=11_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SD_B	159_FIDIA	Sample source:rumen content | Regions:SVR | Host:Cervus nippon	RAW_FILE_NAME(Raw file name)=159_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SD_B	73_FIDIA	Sample source:rumen content | Regions:SVR | Host:Cervus nippon	RAW_FILE_NAME(Raw file name)=73_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SD_B	92_FIDIA	Sample source:rumen content | Regions:SVR | Host:Cervus nippon	RAW_FILE_NAME(Raw file name)=92_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SH_C	07_FIDIA	Sample source:rumen content | Regions:SVR | Host:Ovis aries	RAW_FILE_NAME(Raw file name)=07_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SH_C	113_FIDIA	Sample source:rumen content | Regions:SVR | Host:Ovis aries	RAW_FILE_NAME(Raw file name)=113_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SH_C	138_FIDIA	Sample source:rumen content | Regions:SVR | Host:Ovis aries	RAW_FILE_NAME(Raw file name)=138_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SH_C	42_FIDIA	Sample source:rumen content | Regions:SVR | Host:Ovis aries	RAW_FILE_NAME(Raw file name)=42_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SH_C	89_FIDIA	Sample source:rumen content | Regions:SVR | Host:Ovis aries	RAW_FILE_NAME(Raw file name)=89_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	RD_A	48_FIDIA	Sample source:rumen content | Regions:VSR | Host:Capreolus pygargus	RAW_FILE_NAME(Raw file name)=48_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	RD_A	70_FIDIA	Sample source:rumen content | Regions:VSR | Host:Capreolus pygargus	RAW_FILE_NAME(Raw file name)=70_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	RD_A	74_FIDIA	Sample source:rumen content | Regions:VSR | Host:Capreolus pygargus	RAW_FILE_NAME(Raw file name)=74_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	RD_A	77_FIDIA	Sample source:rumen content | Regions:VSR | Host:Capreolus pygargus	RAW_FILE_NAME(Raw file name)=77_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	RD_A	90_FIDIA	Sample source:rumen content | Regions:VSR | Host:Capreolus pygargus	RAW_FILE_NAME(Raw file name)=90_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SD_B	111_FIDIA	Sample source:rumen content | Regions:VSR | Host:Cervus nippon	RAW_FILE_NAME(Raw file name)=111_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SD_B	146_FIDIA	Sample source:rumen content | Regions:VSR | Host:Cervus nippon	RAW_FILE_NAME(Raw file name)=146_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SD_B	162_FIDIA	Sample source:rumen content | Regions:VSR | Host:Cervus nippon	RAW_FILE_NAME(Raw file name)=162_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SD_B	38_FIDIA	Sample source:rumen content | Regions:VSR | Host:Cervus nippon	RAW_FILE_NAME(Raw file name)=38_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SD_B	82_FIDIA	Sample source:rumen content | Regions:VSR | Host:Cervus nippon	RAW_FILE_NAME(Raw file name)=82_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SH_C	09_FIDIA	Sample source:rumen content | Regions:VSR | Host:Ovis aries	RAW_FILE_NAME(Raw file name)=09_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SH_C	104_FIDIA	Sample source:rumen content | Regions:VSR | Host:Ovis aries	RAW_FILE_NAME(Raw file name)=104_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SH_C	118_FIDIA	Sample source:rumen content | Regions:VSR | Host:Ovis aries	RAW_FILE_NAME(Raw file name)=118_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SH_C	24_FIDIA	Sample source:rumen content | Regions:VSR | Host:Ovis aries	RAW_FILE_NAME(Raw file name)=24_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SH_C	97_FIDIA	Sample source:rumen content | Regions:VSR | Host:Ovis aries	RAW_FILE_NAME(Raw file name)=97_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	RD_A	PLY01_FIDIA	Sample source:rumen liquid | Regions:Liquid | Host:Capreolus pygargus	RAW_FILE_NAME(Raw file name)=PLY01_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	RD_A	PLY02_FIDIA	Sample source:rumen liquid | Regions:Liquid | Host:Capreolus pygargus	RAW_FILE_NAME(Raw file name)=PLY02_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	RD_A	PLY03_FIDIA	Sample source:rumen liquid | Regions:Liquid | Host:Capreolus pygargus	RAW_FILE_NAME(Raw file name)=PLY03_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	RD_A	PLY04_FIDIA	Sample source:rumen liquid | Regions:Liquid | Host:Capreolus pygargus	RAW_FILE_NAME(Raw file name)=PLY04_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	RD_A	PLY05_FIDIA	Sample source:rumen liquid | Regions:Liquid | Host:Capreolus pygargus	RAW_FILE_NAME(Raw file name)=PLY05_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SD_B	MLY01_FIDIA	Sample source:rumen liquid | Regions:Liquid | Host:Cervus nippon	RAW_FILE_NAME(Raw file name)=MLY01_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SD_B	MLY02_FIDIA	Sample source:rumen liquid | Regions:Liquid | Host:Cervus nippon	RAW_FILE_NAME(Raw file name)=MLY02_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SD_B	MLY04_FIDIA	Sample source:rumen liquid | Regions:Liquid | Host:Cervus nippon	RAW_FILE_NAME(Raw file name)=MLY04_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SD_B	MLY05_FIDIA	Sample source:rumen liquid | Regions:Liquid | Host:Cervus nippon	RAW_FILE_NAME(Raw file name)=MLY05_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SH_C	SLY01_FIDIA	Sample source:rumen liquid | Regions:Liquid | Host:Ovis aries	RAW_FILE_NAME(Raw file name)=SLY01_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SH_C	SLY02_FIDIA	Sample source:rumen liquid | Regions:Liquid | Host:Ovis aries	RAW_FILE_NAME(Raw file name)=SLY02_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SH_C	SLY03_FIDIA	Sample source:rumen liquid | Regions:Liquid | Host:Ovis aries	RAW_FILE_NAME(Raw file name)=SLY03_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SH_C	SLY04_FIDIA	Sample source:rumen liquid | Regions:Liquid | Host:Ovis aries	RAW_FILE_NAME(Raw file name)=SLY04_FIDIA.cdf
SUBJECT_SAMPLE_FACTORS           	SH_C	SLY05_FIDIA	Sample source:rumen liquid | Regions:Liquid | Host:Ovis aries	RAW_FILE_NAME(Raw file name)=SLY05_FIDIA.cdf
#COLLECTION
CO:COLLECTION_SUMMARY            	The contents from 11 rumen regions were collected: atrium ruminis (AR; n = 15),
CO:COLLECTION_SUMMARY            	saccus dorsalis (DSR; n = 15), saccus ventralis (VSR; n = 15), saccus cecus
CO:COLLECTION_SUMMARY            	caudodorsalis (SDR; n = 14), saccus cecus caudoventralis (SVR; n = 15), sulcus
CO:COLLECTION_SUMMARY            	cranialis (SCR; n = 15), sulcus longitudinalis sinister (SLSR; n = 14), sulcus
CO:COLLECTION_SUMMARY            	coronarius ventralis (SCVR; n = 15), sulcus caudalis (SAR; n = 15), sulcus
CO:COLLECTION_SUMMARY            	accessorius sinister (SASR; n = 15), and insula ruminis (IR; n = 15). In
CO:COLLECTION_SUMMARY            	addition, the rumen liquid (n = 15) filtered through four layers of cheesecloth
CO:COLLECTION_SUMMARY            	were collected.
CO:SAMPLE_TYPE                   	rumen content and liquid
CO:COLLECTION_METHOD             	Rumen content samples were manually collected from each anatomical region
CO:COLLECTION_METHOD             	through dissection immediately after euthanasia. Rumen liquid was obtained by
CO:COLLECTION_METHOD             	filtering rumen digesta through four layers of cheesecloth.
CO:COLLECTION_LOCATION           	AR :Atrium ruminis; DSR:Saccus dorsalis; VSR:Saccus ventralis; SDR:Saccus cecus
CO:COLLECTION_LOCATION           	caudodorsalis;SVR:Saccus cecus caudoventralis;SCR:Sulcus cranialis; SLSR:Sulcus
CO:COLLECTION_LOCATION           	longitudinalis sinister ;SCVR :Sulcus coronarius ventralis; SAR :Sulcus caudalis
CO:COLLECTION_LOCATION           	:SASR :Sulcus accessorius sinister ; IR:Insula ruminis ; Liquid :Rumen liquid
CO:COLLECTION_FREQUENCY          	15
CO:COLLECTION_DURATION           	1 year
CO:VOLUMEORAMOUNT_COLLECTED      	5ml
CO:STORAGE_CONDITIONS            	-80℃
#TREATMENT
TR:TREATMENT_SUMMARY             	Animals received no experimental treatment and were maintained under identical
TR:TREATMENT_SUMMARY             	feeding and housing conditions.
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	Rumen content was homogenized and acidified to release short-chain fatty acids
SP:SAMPLEPREP_SUMMARY            	(SCFAs). Samples were centrifuged, and the supernatant was collected for
SP:SAMPLEPREP_SUMMARY            	analysis. Rumen liquid samples were filtered through four layers of cheesecloth,
SP:SAMPLEPREP_SUMMARY            	acidified, and centrifuged. All supernatants were transferred into GC vials for
SP:SAMPLEPREP_SUMMARY            	GC-FID analysis without further derivatization
SP:PROCESSING_STORAGE_CONDITIONS 	-80℃
SP:EXTRACT_STORAGE               	Room temperature
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_SUMMARY        	Gas chromatography was performed using an Agilent 7890B GC system with a DB-FFAP
CH:CHROMATOGRAPHY_SUMMARY        	capillary column. Samples (1 μL) were injected in split mode (20:1), nitrogen
CH:CHROMATOGRAPHY_SUMMARY        	was used as the carrier gas, and the oven temperature was programmed from 60°C
CH:CHROMATOGRAPHY_SUMMARY        	to 190°C. Detection was performed using a flame ionization detector at 250°C.
CH:CHROMATOGRAPHY_SUMMARY        	Agilent DB-FFAP (30m x 0.25mm, 0.25um)
CH:INSTRUMENT_NAME               	Agilent 7890B
CH:COLUMN_NAME                   	Agilent DB-FFAP (30m x 0.25mm, 0.25um)
CH:COLUMN_TEMPERATURE            	the initial temperature was set at 60 °C (hold 0 min), followed by a
CH:COLUMN_TEMPERATURE            	temperature ramp of 20 °C/min to 190 °C, where it was held for 3 min before
CH:COLUMN_TEMPERATURE            	returning to initial conditions.
CH:FLOW_GRADIENT                 	-
CH:FLOW_RATE                     	-
CH:SOLVENT_A                     	-
CH:SOLVENT_B                     	-
CH:CHROMATOGRAPHY_TYPE           	GC
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
#MS
MS:INSTRUMENT_NAME               	None
MS:INSTRUMENT_TYPE               	None
MS:MS_TYPE                       	None
MS:MS_COMMENTS                   	No mass spectrometry was used in this study. Samples were analyzed by GC-FID,
MS:MS_COMMENTS                   	and therefore no MS acquisition, MS-based data processing, or feature-assignment
MS:MS_COMMENTS                   	procedures were performed. Peak identification and SCFA quantification were
MS:MS_COMMENTS                   	carried out using chromatographic software only, and no MS-related workflows
MS:MS_COMMENTS                   	were applied.No QC samples were included in this GC-FID SCFA analysis because
MS:MS_COMMENTS                   	the assay was performed directly on individual rumen content and rumen fluid
MS:MS_COMMENTS                   	samples without pooled biological QC or technical QC injections. All samples
MS:MS_COMMENTS                   	were prepared and analyzed under identical experimental conditions. Instrument
MS:MS_COMMENTS                   	performance and stability were ensured through routine calibration of the GC-FID
MS:MS_COMMENTS                   	system, consistent retention time monitoring, and system suitability checks
MS:MS_COMMENTS                   	rather than dedicated QC runs.
MS:ION_MODE                      	None
#MS_METABOLITE_DATA
MS_METABOLITE_DATA:UNITS         	mM
MS_METABOLITE_DATA_START
Samples	108_FIDIA	119_FIDIA	120_FIDIA	163_FIDIA	06_FIDIA	101_FIDIA	125_FIDIA	12_FIDIA	143_FIDIA	115_FIDIA	26_FIDIA	27_FIDIA	72_FIDIA	99_FIDIA	112_FIDIA	130_FIDIA	66_FIDIA	76_FIDIA	93_FIDIA	02_FIDIA	109_FIDIA	16_FIDIA	21_FIDIA	33_FIDIA	105_FIDIA	135_FIDIA	15_FIDIA	28_FIDIA	65_FIDIA	136_FIDIA	156_FIDIA	52_FIDIA	63_FIDIA	80_FIDIA	139_FIDIA	153_FIDIA	23_FIDIA	25_FIDIA	68_FIDIA	141_FIDIA	149_FIDIA	30_FIDIA	31_FIDIA	56_FIDIA	129_FIDIA	154_FIDIA	45_FIDIA	78_FIDIA	88_FIDIA	122_FIDIA	132_FIDIA	53_FIDIA	58_FIDIA	75_FIDIA	10_FIDIA	117_FIDIA	137_FIDIA	19_FIDIA	22_FIDIA	04_FIDIA	102_FIDIA	17_FIDIA	35_FIDIA	85_FIDIA	110_FIDIA	131_FIDIA	161_FIDIA	39_FIDIA	84_FIDIA	100_FIDIA	134_FIDIA	144_FIDIA	51_FIDIA	98_FIDIA	147_FIDIA	57_FIDIA	81_FIDIA	83_FIDIA	91_FIDIA	126_FIDIA	151_FIDIA	41_FIDIA	47_FIDIA	62_FIDIA	03_FIDIA	05_FIDIA	127_FIDIA	145_FIDIA	69_FIDIA	116_FIDIA	18_FIDIA	49_FIDIA	95_FIDIA	106_FIDIA	114_FIDIA	140_FIDIA	142_FIDIA	160_FIDIA	20_FIDIA	29_FIDIA	43_FIDIA	60_FIDIA	107_FIDIA	124_FIDIA	14_FIDIA	152_FIDIA	54_FIDIA	08_FIDIA	121_FIDIA	13_FIDIA	50_FIDIA	59_FIDIA	123_FIDIA	128_FIDIA	133_FIDIA	40_FIDIA	44_FIDIA	157_FIDIA	55_FIDIA	67_FIDIA	94_FIDIA	96_FIDIA	34_FIDIA	36_FIDIA	37_FIDIA	86_FIDIA	87_FIDIA	01_FIDIA	158_FIDIA	32_FIDIA	46_FIDIA	71_FIDIA	148_FIDIA	150_FIDIA	155_FIDIA	64_FIDIA	79_FIDIA	103_FIDIA	11_FIDIA	159_FIDIA	73_FIDIA	92_FIDIA	07_FIDIA	113_FIDIA	138_FIDIA	42_FIDIA	89_FIDIA	48_FIDIA	70_FIDIA	74_FIDIA	77_FIDIA	90_FIDIA	111_FIDIA	146_FIDIA	162_FIDIA	38_FIDIA	82_FIDIA	09_FIDIA	104_FIDIA	118_FIDIA	24_FIDIA	97_FIDIA	PLY01_FIDIA	PLY02_FIDIA	PLY03_FIDIA	PLY04_FIDIA	PLY05_FIDIA	MLY01_FIDIA	MLY02_FIDIA	MLY04_FIDIA	MLY05_FIDIA	SLY01_FIDIA	SLY02_FIDIA	SLY03_FIDIA	SLY04_FIDIA	SLY05_FIDIA
Factors	Sample source:rumen content | Regions:AR | Host:Capreolus pygargus	Sample source:rumen content | Regions:AR | Host:Capreolus pygargus	Sample source:rumen content | Regions:AR | Host:Capreolus pygargus	Sample source:rumen content | Regions:AR | Host:Capreolus pygargus	Sample source:rumen content | Regions:AR | Host:Cervus nippon	Sample source:rumen content | Regions:AR | Host:Cervus nippon	Sample source:rumen content | Regions:AR | Host:Cervus nippon	Sample source:rumen content | Regions:AR | Host:Cervus nippon	Sample source:rumen content | Regions:AR | Host:Cervus nippon	Sample source:rumen content | Regions:AR | Host:Ovis aries	Sample source:rumen content | Regions:AR | Host:Ovis aries	Sample source:rumen content | Regions:AR | Host:Ovis aries	Sample source:rumen content | Regions:AR | Host:Ovis aries	Sample source:rumen content | Regions:AR | Host:Ovis aries	Sample source:rumen content | Regions:DSR | Host:Capreolus pygargus	Sample source:rumen content | Regions:DSR | Host:Capreolus pygargus	Sample source:rumen content | Regions:DSR | Host:Capreolus pygargus	Sample source:rumen content | Regions:DSR | Host:Capreolus pygargus	Sample source:rumen content | Regions:DSR | Host:Capreolus pygargus	Sample source:rumen content | Regions:DSR | Host:Cervus nippon	Sample source:rumen content | Regions:DSR | Host:Cervus nippon	Sample source:rumen content | Regions:DSR | Host:Cervus nippon	Sample source:rumen content | Regions:DSR | Host:Cervus nippon	Sample source:rumen content | Regions:DSR | Host:Cervus nippon	Sample source:rumen content | Regions:DSR | Host:Ovis aries	Sample source:rumen content | Regions:DSR | Host:Ovis aries	Sample source:rumen content | Regions:DSR | Host:Ovis aries	Sample source:rumen content | Regions:DSR | Host:Ovis aries	Sample source:rumen content | Regions:DSR | Host:Ovis aries	Sample source:rumen content | Regions:IR | Host:Capreolus pygargus	Sample source:rumen content | Regions:IR | Host:Capreolus pygargus	Sample source:rumen content | Regions:IR | Host:Capreolus pygargus	Sample source:rumen content | Regions:IR | Host:Capreolus pygargus	Sample source:rumen content | Regions:IR | Host:Capreolus pygargus	Sample source:rumen content | Regions:IR | Host:Cervus nippon	Sample source:rumen content | Regions:IR | Host:Cervus nippon	Sample source:rumen content | Regions:IR | Host:Cervus nippon	Sample source:rumen content | Regions:IR | Host:Cervus nippon	Sample source:rumen content | Regions:IR | Host:Cervus nippon	Sample source:rumen content | Regions:IR | Host:Ovis aries	Sample source:rumen content | Regions:IR | Host:Ovis aries	Sample source:rumen content | Regions:IR | Host:Ovis aries	Sample source:rumen content | Regions:IR | Host:Ovis aries	Sample source:rumen content | Regions:IR | Host:Ovis aries	Sample source:rumen content | Regions:SAR | Host:Capreolus pygargus	Sample source:rumen content | Regions:SAR | Host:Capreolus pygargus	Sample source:rumen content | Regions:SAR | Host:Capreolus pygargus	Sample source:rumen content | Regions:SAR | Host:Capreolus pygargus	Sample source:rumen content | Regions:SAR | Host:Capreolus pygargus	Sample source:rumen content | Regions:SAR | Host:Cervus nippon	Sample source:rumen content | Regions:SAR | Host:Cervus nippon	Sample source:rumen content | Regions:SAR | Host:Cervus nippon	Sample source:rumen content | Regions:SAR | Host:Cervus nippon	Sample source:rumen content | Regions:SAR | Host:Cervus nippon	Sample source:rumen content | Regions:SAR | Host:Ovis aries	Sample source:rumen content | Regions:SAR | Host:Ovis aries	Sample source:rumen content | Regions:SAR | Host:Ovis aries	Sample source:rumen content | Regions:SAR | Host:Ovis aries	Sample source:rumen content | Regions:SAR | Host:Ovis aries	Sample source:rumen content | Regions:SASR | Host:Capreolus pygargus	Sample source:rumen content | Regions:SASR | Host:Capreolus pygargus	Sample source:rumen content | Regions:SASR | Host:Capreolus pygargus	Sample source:rumen content | Regions:SASR | Host:Capreolus pygargus	Sample source:rumen content | Regions:SASR | Host:Capreolus pygargus	Sample source:rumen content | Regions:SASR | Host:Cervus nippon	Sample source:rumen content | Regions:SASR | Host:Cervus nippon	Sample source:rumen content | Regions:SASR | Host:Cervus nippon	Sample source:rumen content | Regions:SASR | Host:Cervus nippon	Sample source:rumen content | Regions:SASR | Host:Cervus nippon	Sample source:rumen content | Regions:SASR | Host:Ovis aries	Sample source:rumen content | Regions:SASR | Host:Ovis aries	Sample source:rumen content | Regions:SASR | Host:Ovis aries	Sample source:rumen content | Regions:SASR | Host:Ovis aries	Sample source:rumen content | Regions:SASR | Host:Ovis aries	Sample source:rumen content | Regions:SCR | Host:Capreolus pygargus	Sample source:rumen content | Regions:SCR | Host:Capreolus pygargus	Sample source:rumen content | Regions:SCR | Host:Capreolus pygargus	Sample source:rumen content | Regions:SCR | Host:Capreolus pygargus	Sample source:rumen content | Regions:SCR | Host:Capreolus pygargus	Sample source:rumen content | Regions:SCR | Host:Cervus nippon	Sample source:rumen content | Regions:SCR | Host:Cervus nippon	Sample source:rumen content | Regions:SCR | Host:Cervus nippon	Sample source:rumen content | Regions:SCR | Host:Cervus nippon	Sample source:rumen content | Regions:SCR | Host:Cervus nippon	Sample source:rumen content | Regions:SCR | Host:Ovis aries	Sample source:rumen content | Regions:SCR | Host:Ovis aries	Sample source:rumen content | Regions:SCR | Host:Ovis aries	Sample source:rumen content | Regions:SCR | Host:Ovis aries	Sample source:rumen content | Regions:SCR | Host:Ovis aries	Sample source:rumen content | Regions:SCVR | Host:Capreolus pygargus	Sample source:rumen content | Regions:SCVR | Host:Capreolus pygargus	Sample source:rumen content | Regions:SCVR | Host:Capreolus pygargus	Sample source:rumen content | Regions:SCVR | Host:Capreolus pygargus	Sample source:rumen content | Regions:SCVR | Host:Cervus nippon	Sample source:rumen content | Regions:SCVR | Host:Cervus nippon	Sample source:rumen content | Regions:SCVR | Host:Cervus nippon	Sample source:rumen content | Regions:SCVR | Host:Cervus nippon	Sample source:rumen content | Regions:SCVR | Host:Ovis aries	Sample source:rumen content | Regions:SCVR | Host:Ovis aries	Sample source:rumen content | Regions:SCVR | Host:Ovis aries	Sample source:rumen content | Regions:SCVR | Host:Ovis aries	Sample source:rumen content | Regions:SCVR | Host:Ovis aries	Sample source:rumen content | Regions:SDR | Host:Capreolus pygargus	Sample source:rumen content | Regions:SDR | Host:Capreolus pygargus	Sample source:rumen content | Regions:SDR | Host:Capreolus pygargus	Sample source:rumen content | Regions:SDR | Host:Capreolus pygargus	Sample source:rumen content | Regions:SDR | Host:Capreolus pygargus	Sample source:rumen content | Regions:SDR | Host:Cervus nippon	Sample source:rumen content | Regions:SDR | Host:Cervus nippon	Sample source:rumen content | Regions:SDR | Host:Cervus nippon	Sample source:rumen content | Regions:SDR | Host:Cervus nippon	Sample source:rumen content | Regions:SDR | Host:Cervus nippon	Sample source:rumen content | Regions:SDR | Host:Ovis aries	Sample source:rumen content | Regions:SDR | Host:Ovis aries	Sample source:rumen content | Regions:SDR | Host:Ovis aries	Sample source:rumen content | Regions:SDR | Host:Ovis aries	Sample source:rumen content | Regions:SDR | Host:Ovis aries	Sample source:rumen content | Regions:SLSR | Host:Capreolus pygargus	Sample source:rumen content | Regions:SLSR | Host:Capreolus pygargus	Sample source:rumen content | Regions:SLSR | Host:Capreolus pygargus	Sample source:rumen content | Regions:SLSR | Host:Capreolus pygargus	Sample source:rumen content | Regions:SLSR | Host:Capreolus pygargus	Sample source:rumen content | Regions:SLSR | Host:Cervus nippon	Sample source:rumen content | Regions:SLSR | Host:Cervus nippon	Sample source:rumen content | Regions:SLSR | Host:Cervus nippon	Sample source:rumen content | Regions:SLSR | Host:Cervus nippon	Sample source:rumen content | Regions:SLSR | Host:Cervus nippon	Sample source:rumen content | Regions:SLSR | Host:Ovis aries	Sample source:rumen content | Regions:SLSR | Host:Ovis aries	Sample source:rumen content | Regions:SLSR | Host:Ovis aries	Sample source:rumen content | Regions:SLSR | Host:Ovis aries	Sample source:rumen content | Regions:SLSR | Host:Ovis aries	Sample source:rumen content | Regions:SVR | Host:Capreolus pygargus	Sample source:rumen content | Regions:SVR | Host:Capreolus pygargus	Sample source:rumen content | Regions:SVR | Host:Capreolus pygargus	Sample source:rumen content | Regions:SVR | Host:Capreolus pygargus	Sample source:rumen content | Regions:SVR | Host:Capreolus pygargus	Sample source:rumen content | Regions:SVR | Host:Cervus nippon	Sample source:rumen content | Regions:SVR | Host:Cervus nippon	Sample source:rumen content | Regions:SVR | Host:Cervus nippon	Sample source:rumen content | Regions:SVR | Host:Cervus nippon	Sample source:rumen content | Regions:SVR | Host:Cervus nippon	Sample source:rumen content | Regions:SVR | Host:Ovis aries	Sample source:rumen content | Regions:SVR | Host:Ovis aries	Sample source:rumen content | Regions:SVR | Host:Ovis aries	Sample source:rumen content | Regions:SVR | Host:Ovis aries	Sample source:rumen content | Regions:SVR | Host:Ovis aries	Sample source:rumen content | Regions:VSR | Host:Capreolus pygargus	Sample source:rumen content | Regions:VSR | Host:Capreolus pygargus	Sample source:rumen content | Regions:VSR | Host:Capreolus pygargus	Sample source:rumen content | Regions:VSR | Host:Capreolus pygargus	Sample source:rumen content | Regions:VSR | Host:Capreolus pygargus	Sample source:rumen content | Regions:VSR | Host:Cervus nippon	Sample source:rumen content | Regions:VSR | Host:Cervus nippon	Sample source:rumen content | Regions:VSR | Host:Cervus nippon	Sample source:rumen content | Regions:VSR | Host:Cervus nippon	Sample source:rumen content | Regions:VSR | Host:Cervus nippon	Sample source:rumen content | Regions:VSR | Host:Ovis aries	Sample source:rumen content | Regions:VSR | Host:Ovis aries	Sample source:rumen content | Regions:VSR | Host:Ovis aries	Sample source:rumen content | Regions:VSR | Host:Ovis aries	Sample source:rumen content | Regions:VSR | Host:Ovis aries	Sample source:rumen liquid | Regions:Liquid | Host:Capreolus pygargus	Sample source:rumen liquid | Regions:Liquid | Host:Capreolus pygargus	Sample source:rumen liquid | Regions:Liquid | Host:Capreolus pygargus	Sample source:rumen liquid | Regions:Liquid | Host:Capreolus pygargus	Sample source:rumen liquid | Regions:Liquid | Host:Capreolus pygargus	Sample source:rumen liquid | Regions:Liquid | Host:Cervus nippon	Sample source:rumen liquid | Regions:Liquid | Host:Cervus nippon	Sample source:rumen liquid | Regions:Liquid | Host:Cervus nippon	Sample source:rumen liquid | Regions:Liquid | Host:Cervus nippon	Sample source:rumen liquid | Regions:Liquid | Host:Ovis aries	Sample source:rumen liquid | Regions:Liquid | Host:Ovis aries	Sample source:rumen liquid | Regions:Liquid | Host:Ovis aries	Sample source:rumen liquid | Regions:Liquid | Host:Ovis aries	Sample source:rumen liquid | Regions:Liquid | Host:Ovis aries	
Acetate	3.6330	4.6000	4.3880	4.6810	6.7860	3.7030	4.6230	5.6600	3.8430	3.5440	5.5920	5.9030	3.6340	2.9780	3.5160	3.5040	4.9690	4.4810	2.8850	9.4820	3.8420	5.9950	6.8100	5.9380	3.1140	3.3260	4.8760	5.6960	9.0000	2.9650	3.5680	6.9020	6.3980	4.2940	4.1550	3.9920	9.1510	6.3570	4.7280	3.3300	3.2040	4.8780	4.6250	5.1550	3.8950	3.7010	4.2910	3.2090	3.3250	4.6360	5.3390	4.4950	5.4510	5.3150	5.7710	4.4680	3.2440	5.0250	5.4910	6.3500	4.5530	5.4390	6.7230	3.3690	3.9910	4.4950	3.8970	5.8740	3.2180	3.3830	3.5080	3.5300	4.7590	3.4400	3.1460	4.8640	4.7040	3.8810	3.5130	4.2360	3.2650	5.1680	5.6170	5.6660	5.7430	4.9920	3.7370	3.3870	4.1750	4.8010	6.0410	5.3570	3.1220	5.3490	3.8520	4.1470	3.6770	4.0650	5.2090	5.4620	6.0620	9.3190	3.6220	3.9440	6.2600	3.0240	4.0700	6.6710	5.9600	6.5250	5.2840	6.7540	3.4170	4.0320	3.3650	4.3450	4.8780	3.5080	4.5000	2.2390	2.7590	4.5830	4.6100	6.6520	6.4030	3.6430	3.8940	7.7700	3.0110	5.1940	6.3700	3.3780	3.0630	3.1220	2.9240	5.0140	3.6320	4.2980	6.6040	3.5880	4.0180	2.8900	4.7570	2.9070	2.9220	4.6030	3.7200	4.4880	3.8800	4.2690	3.4470	3.7810	4.0180	3.7400	7.3260	6.2000	3.7960	4.1050	2.8180	4.2830	5.2960	3.8150	122.7950	190.8440	151.0120	143.7890	126.3370	194.2130	194.0156	167.9710	172.0710	156.7060	203.3640	216.3010	314.1010	185.2590
Butyric acid	0.5460	0.6060	0.5900	0.8160	1.2100	0.5660	0.6170	0.8450	0.6170	0.5410	0.7680	0.9330	0.6110	0.7540	0.5540	0.5630	0.6920	0.6120	0.5490	1.6250	0.5750	0.8340	0.7650	0.6430	0.6520	0.6260	0.8650	0.8260	1.9240	0.5470	0.5710	0.7020	1.0720	0.5890	0.6620	0.5490	0.9030	0.6430	0.5980	0.5800	0.5420	0.6330	0.6120	0.9050	0.5800	0.6030	0.5880	0.5510	0.5560	0.6160	0.7150	0.5890	0.9220	0.6400	1.1370	0.7270	0.5910	0.8240	0.7960	1.1510	0.6280	0.6940	0.6850	0.5550	0.5850	0.6000	0.6310	0.7370	0.5720		0.6240	0.6860	0.7570	0.5540	0.5670	1.4560	0.6380	0.5760	0.6170	0.6140	0.5420	0.6530	0.6620	0.9090	1.4050	1.2000	0.6210	0.5360	0.6560	0.7390	0.7350	0.6040	0.5860	0.6520	0.5640	0.6300	0.5490	0.5770	0.8680	0.7280	1.0740	4.5310	0.5550	0.5810	0.7150	0.5420	0.5770	1.0910	0.7420	0.9210	0.6230	1.7920	0.5870	0.6290	0.5940	0.6230	0.7950	0.5900	0.8690	0.5400	0.5390	0.5990	0.6320	1.5180	0.6790	0.5580	0.5770	1.9460	0.6930	0.6030	0.6500	0.5670	0.5450	0.5570	0.5420	0.8850	0.5540	0.5390	0.9350	0.5710	0.5780	0.5470	1.0470	0.5550	0.5760	0.6460	0.5700	0.5700	0.5650	0.5900	0.5590	0.5700	0.5800	0.6250	0.5550	0.6490	0.5970	0.6790	0.5550	0.6850	0.7030	0.5650	18.5310	30.3360	22.6440	22.1710	19.2910	33.3710	61.6970	54.6590	72.7990	44.5260	58.2160	65.1650	94.8770	55.3930
Isobutyric acid	0.0810	0.0310	0.0360	0.1070	0.0420	0.0860	0.0620	0.1130	0.1420	0.0800	0.1290	0.1370	0.0690	0.0500	0.0270	0.0220	0.0380	0.0740	0.0860	0.1020	0.0680	0.2160	0.1210	0.1240	0.0860	0.0850	0.1750	0.1380	0.0900	0.0450	0.0670	0.1110	0.0230	0.0900	0.0670	0.1190	0.2100	0.1090	0.0600	0.1510	0.1000	0.0960	0.1100	0.0620	0.0500	0.0770	0.0470	0.0310	0.0310	0.0230	0.0450	0.0410	0.0390	0.0340	0.2080	0.1010	0.0870	0.1880	0.1360	0.0870	0.0370	0.0940	0.0510	0.0310	0.0750	0.1430	0.1560	0.0870	0.0910	0.0240	0.1250	0.1010	0.1040	0.1000	0.0470	0.1600	0.0910	0.0370	0.1110	0.0790	0.0670	0.0600	0.1080	0.0390	0.1200	0.1000	0.1100	0.1670	0.0710	0.0450	0.1370	0.0370	0.1070	0.0740	0.0720	0.1380	0.1070	0.0410	0.1550	0.0960	0.1380	0.4230	0.0550	0.0300	0.0460	0.0550	0.0250	0.0930	0.0660	0.1280	0.0400	0.0710	0.0610	0.1050	0.1180	0.0790	0.1000	0.0660	0.0410	0.0680	0.0600	0.1060	0.1060	0.1570	0.1120	0.0600	0.0590	0.1960	0.0740	0.0500	0.0940	0.0750	0.0450	0.0290	0.0360	0.0380	0.0560	0.0810	0.1610	0.0910	0.0280	0.0110	0.0940	0.0680	0.1480	0.0840	0.0240	0.0240	0.0190	0.1060	0.0320	0.0680	0.1140	0.0740	0.1700	0.0870	0.0420	0.1140	0.0630	0.1670	0.1280	0.0300	0.9900	1.6560	1.2150	1.1990	1.0320	1.8670	2.4640	2.0390	2.9210	3.7750	5.2010	5.4720	8.0460	4.6660
Isovaleric acid	0.1910	0.1740	0.1210	0.1330	0.1260	0.2270	0.1320	0.1040	0.1040	0.2630	0.1730	0.2070	0.1630	0.1570	0.1800	0.1210	0.1150	0.2120	0.1630	0.1910	0.1250	0.1080	0.2550	0.1820	0.1600	0.2430	0.1550	0.1710	0.1940	0.1590	0.1610	0.1140	0.1940	0.1230	0.1090	0.0950	0.1380	0.0980	0.1080	0.1370	0.1380	0.1410	0.1550	0.1970	0.1580	0.2020	0.1080	0.1230	0.1640	0.1330	0.1430	0.1050	0.1960	0.0940	0.1940	0.1510	0.2750	0.1490	0.1820	0.1070	0.1650	0.2160	0.1750	0.1000	0.1230	0.1100	0.1060	0.1870	0.1290		0.3300	0.1430	0.1660	0.1900	0.0930	0.2630	0.1870	0.1320	0.2620	0.1180	0.1330	0.1190	0.2420	0.1210	0.2080	0.1960	0.1880	0.1360	0.2490	0.1260	0.1190	0.1100	0.2650	0.1530	0.2630	0.1170	0.1080	0.1520	0.2610	0.1720	0.3240	0.5830		0.1250	0.2070	0.2030	0.1080	0.1090	0.2440	0.1020	0.1250	0.1590	0.1050	0.2590	0.1180	0.1850	0.1860	0.1500	0.1270	0.1080	0.1460	0.2330	0.1910	0.2760	0.1830	0.1180	0.1020	0.3440	0.1340	0.1200	0.1550	0.1230	0.1860	0.1120	0.1610	0.1200	0.1230	0.1490	0.1080	0.0960	0.1230	0.1170	0.1610	0.2040	0.2790	0.1560	0.1640	0.1340	0.0970	0.1510	0.1070	0.1220	0.1190	0.1050	0.1560	0.1100	0.1700	0.1480	0.1650	0.2320	0.1380	0.1400	0.8250	1.4460	1.0160	1.0060	0.8550	0.9970	1.9210	1.6770	2.2800	3.5830	4.6960	5.3890	7.8840	4.5510
Propionate	1.3140	1.4760	1.4790	2.0930	2.6890	1.5230	1.8300	2.1270	1.8320	1.4030	1.7370	2.0330	1.3420	1.1790	1.2840	1.2980	1.6800	1.7740	1.2650	3.6280	1.5000	2.2050	2.2750	1.9340	1.2390	1.3090	1.5950	1.8120	3.6440	1.2470	1.4960	2.1550	3.3150	1.7300	1.9730	1.7770	2.9790	1.9990	1.6320	1.4780	1.3670	1.4270	1.4190	2.3520	1.4180	1.5570	1.4310	1.2600	1.3380	1.7450	2.2210	1.4400	2.8460	2.0310	1.9120	1.6050	1.3150	1.5760	1.6390	2.6890	1.6470	1.6000	1.8650	1.3170	1.5150	1.9110	1.6740	2.4590	1.3490	1.2340	1.4070	1.4820	1.6510	1.2910	1.3450	2.5370	1.9900	1.4560	1.4070	1.7580	1.4560	2.0930	2.2050	2.9470	1.9710	1.7610	1.4480	1.4300	1.4320	1.7750	1.7200	1.6300	1.3110	1.9400	1.5750	2.0400	1.7600	1.6290	1.6200	1.6060	2.1590	5.1280	1.3370	1.3980	1.6350	1.2990	1.4850	2.6920	2.0940	2.4080	1.9540	4.8410	1.3520	1.5720	1.4080	1.3920	1.7140	1.4230	2.0860	1.1280	1.1950	1.5360	1.4630	2.6070	2.0880	1.3920	1.7100	2.6010	1.2830	1.5220	2.2000	1.2860	1.2710	1.2730	1.2360	2.0950	1.3520	1.7150	2.4630	1.5630	1.3610	1.1730	1.6500	1.2200	1.2850	1.4820	1.3690	1.3420	1.3190	1.6350	1.3380	1.4370	1.5610	1.7200	4.2300	2.1120	1.6480	1.4200	1.2410	1.4810	1.5740	1.3440	29.7590	48.0550	36.7410	35.4770	30.9640	70.5630	132.1020	58.6720	60.5300	41.8570	55.3600	60.0350	88.2630	51.3790
MS_METABOLITE_DATA_END
#METABOLITES
METABOLITES_START
metabolite_name	pubchem_id	inchi_key	kegg_id	other_id	other_id_type	ri	ri_type	moverz_quant	
Acetate	175		C00033						
Butyric acid	264		C00109						
Isobutyric acid	6590		C01188						
Isovaleric acid	10430		C08262						
Propionate	104745		C00163						
METABOLITES_END
#END