Subject Summary:

Staphylococcus aureus strain UAMS-1, a clinical osteomyelitis isolate, will be used in this study. We will also be testing isogenic nitric oxide synthase, SrrAB, and SrrAB nos double mutants and complement strains of the single nos mutant and the double mutant. Complementation was completed on a plasmid with chloramphenicol resistance and both the wildtype and all non complement strains contain empty vector.