Acquisition Info
	Method Path:	D:\Analyst Data\Projects\TM_Methods\Acquisition Methods\LIP_15min_MSMS_Jenny_new_20min_Positive.dam
	Batch Path:	D:\Analyst Data\Projects\TM_Methods\Batch\20150413_Ring_Trial_Positive.dab
	Acquisition Date:	Monday, April 13, 2015
	Acquisition Time:	10:22:04 PM
	User Name:	ABI_5600
	Acquisition Method:	LIP_15min_MSMS_Jenny_new_20min_Positive.dam
	Rack:	1
	Plate:	1
	Vial:	3
	Injection Volume:	5.00

Quantitation Info
	Sample Type:	Unknown
	Dilution Factor:	1.0000

Log Info
	Integrated System:	Shimadzu Controller,  CBM20A
	Serial#:	L20235154646
	ROM Version:	2.40
	Pressure Units:	psi
	Pump:	Shimadzu LC30AD
	Serial#:	L20555151103
	ROM Version:	3.00
	Pump:	Shimadzu LC30AD
	Serial#:	L20555151046
	ROM Version:	3.00
	AutoSampler:	Shimadzu SIL30AC
	Serial#:	L20565250433
	ROM Version:	3.10
	Column Oven:	Shimadzu CTO30A
	Serial#:	L20575150303
	ROM Version:	3.00
	Injection Volume used:	5.00 µl

	Time (from start of run):	00:00:01
	Mass Spectrometer:	TripleTOF 5600-1
	Config Table Version:	02
	Firmware Version:	MIA3000 ------- MIL4000 MIB3000
	Component Name:	Hybrid Quadrupole-TOF LC/MS/MS Mass Spectrometer
	Component ID:	TripleTOF 5600+
	Manufacturer:	AB Sciex Instruments
	Model:	5016230/L
	Serial Number:	BN20031205H
	Source Housing:	DuoSpray Ion Source
	Static Accumulation.:	N/A
	Mass Spectrometer:	TripleTOF 5600-1
	Start of Run - Detailed Status:	N/A
	Vacuum Status:	At Pressure
	Vacuum Gauge (10e-5 Torr):	3.6
	Backing Pump:	Ok
	Q1 Turbo Pump:	Normal
	Q2/TOF Turbo Pump:	Normal
	Sample Introduction Status:	Ready
	Source/Ion Path Electronics:	On
	Source Type:	DuoSpray Ion Source
	Source Temperature (at setpoint):	450.0 C
	Source Exhaust Pump:	Ok
	Injection Manifold:	Bypass

	Time (from start of run):	00:20:35
	Mass Spectrometer:	TripleTOF 5600-1
	End of Run - Detailed Status:	N/A
	Vacuum Status:	At Pressure
	Vacuum Gauge (10e-5 Torr):	3.6
	Backing Pump:	Ok
	Q1 Turbo Pump:	Normal
	Q2/TOF Turbo Pump:	Normal
	Sample Introduction Status:	Ready
	Source/Ion Path Electronics:	On
	Source Type:	DuoSpray Ion Source
	Source Temperature (at setpoint):	450.0 C
	Source Exhaust Pump:	Ok
	Injection Manifold:	Bypass

IDA
	With intensity greater than:	250
	Switch after:	15 spectra
	Use advanced settings:	True
	Always exclude:	True
	Exclude for:	9 sec
	Mass tolerance units:	mDa
	Mass tolerance:	50
	Use inclusion list:	False
	Use exclusion list:	False
	Ignore peaks within:	6 Da
	Real time:	None
	Spectrum file:	aaa
	Dynamic background subtraction:	True
	Fragment intensity multiplier:	2
	Maximum accumulation:	2 sec
	Allow standard filters for Smart IDA:	True
	Never exclude former target ions:	True
	Exclude after occurences:	1

Period 1, Experiment 1
	Experiment Type:	TOF MS
	Num. Cycles:	1684
	Polarity:	Positive
	Period Cycle Time:	2450 ms
	Pulser Frequency:	16.906 kHz
	Accumulation Time:	150.0 ms

	Experiment Parameters
	Parameter	Value
	CUR	30.000
	GS1	40.000
	GS2	45.000
	ISVF	5500.000
	TEM	450.000

	Mass Range Parameters
	Parameter	Value
	CE	10.000
	DP	60.000
	IDIx	0.000
	IDUx	5.000
	IRDx	15000.000
	IRWx	10000.000
	IWIx	0.000
	IWUx	5.000
	XA1	139.530

	Start Mass:	50.0
	End Mass:	1200.0

	RF Transmission
	Mass	Time (%)
	40.0	33.0
	120.0	33.0
	390.0	34.0

Period 1, Experiment 2
	Experiment Type:	TOF MS^2
	Num. Cycles:	1684
	Polarity:	Positive
	Product :	IDA
	Period Cycle Time:	2450 ms
	Pulser Frequency:	16.906 kHz
	Accumulation Time:	150.0 ms

	Experiment Parameters
	Parameter	Value
	CUR	30.000
	GS1	40.000
	GS2	45.000
	ISVF	5500.000
	TEM	450.000

	Mass Range Parameters
	Parameter	Value
	CE	30.000
	CES	20.000
	DP	60.000
	IDIx	0.000
	IDUx	5.000
	IRD	66.633
	IRDx	15000.000
	IRW	24.917
	IRWx	10000.000
	IWIx	0.000
	IWUx	5.000
	XA1	70.160

	Start Mass:	50.0
	End Mass:	1200.0

	RF Transmission
	Mass	Time (%)
	40.0	50.0
	120.0	50.0

Period 1, Experiment 3
	Experiment Type:	TOF MS^2
	Num. Cycles:	1684
	Polarity:	Positive
	Product :	IDA
	Period Cycle Time:	2450 ms
	Pulser Frequency:	16.906 kHz
	Accumulation Time:	150.0 ms

	Experiment Parameters
	Parameter	Value
	CUR	30.000
	GS1	40.000
	GS2	45.000
	ISVF	5500.000
	TEM	450.000

	Mass Range Parameters
	Parameter	Value
	CE	30.000
	CES	20.000
	DP	60.000
	IDIx	0.000
	IDUx	5.000
	IRD	66.633
	IRDx	15000.000
	IRW	24.917
	IRWx	10000.000
	IWIx	0.000
	IWUx	5.000
	XA1	70.320

	Start Mass:	50.0
	End Mass:	1200.0

	RF Transmission
	Mass	Time (%)
	40.0	50.0
	120.0	50.0

Period 1, Experiment 4
	Experiment Type:	TOF MS^2
	Num. Cycles:	1684
	Polarity:	Positive
	Product :	IDA
	Period Cycle Time:	2450 ms
	Pulser Frequency:	16.906 kHz
	Accumulation Time:	150.0 ms

	Experiment Parameters
	Parameter	Value
	CUR	30.000
	GS1	40.000
	GS2	45.000
	ISVF	5500.000
	TEM	450.000

	Mass Range Parameters
	Parameter	Value
	CE	30.000
	CES	20.000
	DP	60.000
	IDIx	0.000
	IDUx	5.000
	IRD	66.633
	IRDx	15000.000
	IRW	24.917
	IRWx	10000.000
	IWIx	0.000
	IWUx	5.000
	XA1	70.480

	Start Mass:	50.0
	End Mass:	1200.0

	RF Transmission
	Mass	Time (%)
	40.0	50.0
	120.0	50.0

Period 1, Experiment 5
	Experiment Type:	TOF MS^2
	Num. Cycles:	1684
	Polarity:	Positive
	Product :	IDA
	Period Cycle Time:	2450 ms
	Pulser Frequency:	16.906 kHz
	Accumulation Time:	150.0 ms

	Experiment Parameters
	Parameter	Value
	CUR	30.000
	GS1	40.000
	GS2	45.000
	ISVF	5500.000
	TEM	450.000

	Mass Range Parameters
	Parameter	Value
	CE	30.000
	CES	20.000
	DP	60.000
	IDIx	0.000
	IDUx	5.000
	IRD	66.633
	IRDx	15000.000
	IRW	24.917
	IRWx	10000.000
	IWIx	0.000
	IWUx	5.000
	XA1	70.640

	Start Mass:	50.0
	End Mass:	1200.0

	RF Transmission
	Mass	Time (%)
	40.0	50.0
	120.0	50.0

Period 1, Experiment 6
	Experiment Type:	TOF MS^2
	Num. Cycles:	1684
	Polarity:	Positive
	Product :	IDA
	Period Cycle Time:	2450 ms
	Pulser Frequency:	16.906 kHz
	Accumulation Time:	150.0 ms

	Experiment Parameters
	Parameter	Value
	CUR	30.000
	GS1	40.000
	GS2	45.000
	ISVF	5500.000
	TEM	450.000

	Mass Range Parameters
	Parameter	Value
	CE	30.000
	CES	20.000
	DP	60.000
	IDIx	0.000
	IDUx	5.000
	IRD	66.633
	IRDx	15000.000
	IRW	24.917
	IRWx	10000.000
	IWIx	0.000
	IWUx	5.000
	XA1	70.800

	Start Mass:	50.0
	End Mass:	1200.0

	RF Transmission
	Mass	Time (%)
	40.0	50.0
	120.0	50.0

Period 1, Experiment 7
	Experiment Type:	TOF MS^2
	Num. Cycles:	1684
	Polarity:	Positive
	Product :	IDA
	Period Cycle Time:	2450 ms
	Pulser Frequency:	16.906 kHz
	Accumulation Time:	150.0 ms

	Experiment Parameters
	Parameter	Value
	CUR	30.000
	GS1	40.000
	GS2	45.000
	ISVF	5500.000
	TEM	450.000

	Mass Range Parameters
	Parameter	Value
	CE	30.000
	CES	20.000
	DP	60.000
	IDIx	0.000
	IDUx	5.000
	IRD	66.633
	IRDx	15000.000
	IRW	24.917
	IRWx	10000.000
	IWIx	0.000
	IWUx	5.000
	XA1	70.960

	Start Mass:	50.0
	End Mass:	1200.0

	RF Transmission
	Mass	Time (%)
	40.0	50.0
	120.0	50.0

Period 1, Experiment 8
	Experiment Type:	TOF MS^2
	Num. Cycles:	1684
	Polarity:	Positive
	Product :	IDA
	Period Cycle Time:	2450 ms
	Pulser Frequency:	16.906 kHz
	Accumulation Time:	150.0 ms

	Experiment Parameters
	Parameter	Value
	CUR	30.000
	GS1	40.000
	GS2	45.000
	ISVF	5500.000
	TEM	450.000

	Mass Range Parameters
	Parameter	Value
	CE	30.000
	CES	20.000
	DP	60.000
	IDIx	0.000
	IDUx	5.000
	IRD	66.633
	IRDx	15000.000
	IRW	24.917
	IRWx	10000.000
	IWIx	0.000
	IWUx	5.000
	XA1	71.120

	Start Mass:	50.0
	End Mass:	1200.0

	RF Transmission
	Mass	Time (%)
	40.0	50.0
	120.0	50.0

Period 1, Experiment 9
	Experiment Type:	TOF MS^2
	Num. Cycles:	1684
	Polarity:	Positive
	Product :	IDA
	Period Cycle Time:	2450 ms
	Pulser Frequency:	16.906 kHz
	Accumulation Time:	150.0 ms

	Experiment Parameters
	Parameter	Value
	CUR	30.000
	GS1	40.000
	GS2	45.000
	ISVF	5500.000
	TEM	450.000

	Mass Range Parameters
	Parameter	Value
	CE	30.000
	CES	20.000
	DP	60.000
	IDIx	0.000
	IDUx	5.000
	IRD	66.633
	IRDx	15000.000
	IRW	24.917
	IRWx	10000.000
	IWIx	0.000
	IWUx	5.000
	XA1	71.280

	Start Mass:	50.0
	End Mass:	1200.0

	RF Transmission
	Mass	Time (%)
	40.0	50.0
	120.0	50.0

Period 1, Experiment 10
	Experiment Type:	TOF MS^2
	Num. Cycles:	1684
	Polarity:	Positive
	Product :	IDA
	Period Cycle Time:	2450 ms
	Pulser Frequency:	16.906 kHz
	Accumulation Time:	150.0 ms

	Experiment Parameters
	Parameter	Value
	CUR	30.000
	GS1	40.000
	GS2	45.000
	ISVF	5500.000
	TEM	450.000

	Mass Range Parameters
	Parameter	Value
	CE	30.000
	CES	20.000
	DP	60.000
	IDIx	0.000
	IDUx	5.000
	IRD	66.633
	IRDx	15000.000
	IRW	24.917
	IRWx	10000.000
	IWIx	0.000
	IWUx	5.000
	XA1	71.440

	Start Mass:	50.0
	End Mass:	1200.0

	RF Transmission
	Mass	Time (%)
	40.0	50.0
	120.0	50.0

Period 1, Experiment 11
	Experiment Type:	TOF MS^2
	Num. Cycles:	1684
	Polarity:	Positive
	Product :	IDA
	Period Cycle Time:	2450 ms
	Pulser Frequency:	16.906 kHz
	Accumulation Time:	150.0 ms

	Experiment Parameters
	Parameter	Value
	CUR	30.000
	GS1	40.000
	GS2	45.000
	ISVF	5500.000
	TEM	450.000

	Mass Range Parameters
	Parameter	Value
	CE	30.000
	CES	20.000
	DP	60.000
	IDIx	0.000
	IDUx	5.000
	IRD	66.633
	IRDx	15000.000
	IRW	24.917
	IRWx	10000.000
	IWIx	0.000
	IWUx	5.000
	XA1	71.600

	Start Mass:	50.0
	End Mass:	1200.0

	RF Transmission
	Mass	Time (%)
	40.0	50.0
	120.0	50.0

Period 1, Experiment 12
	Experiment Type:	TOF MS^2
	Num. Cycles:	1684
	Polarity:	Positive
	Product :	IDA
	Period Cycle Time:	2450 ms
	Pulser Frequency:	16.906 kHz
	Accumulation Time:	150.0 ms

	Experiment Parameters
	Parameter	Value
	CUR	30.000
	GS1	40.000
	GS2	45.000
	ISVF	5500.000
	TEM	450.000

	Mass Range Parameters
	Parameter	Value
	CE	30.000
	CES	20.000
	DP	60.000
	IDIx	0.000
	IDUx	5.000
	IRD	66.633
	IRDx	15000.000
	IRW	24.917
	IRWx	10000.000
	IWIx	0.000
	IWUx	5.000
	XA1	71.760

	Start Mass:	50.0
	End Mass:	1200.0

	RF Transmission
	Mass	Time (%)
	40.0	50.0
	120.0	50.0

Period 1, Experiment 13
	Experiment Type:	TOF MS^2
	Num. Cycles:	1684
	Polarity:	Positive
	Product :	IDA
	Period Cycle Time:	2450 ms
	Pulser Frequency:	16.906 kHz
	Accumulation Time:	150.0 ms

	Experiment Parameters
	Parameter	Value
	CUR	30.000
	GS1	40.000
	GS2	45.000
	ISVF	5500.000
	TEM	450.000

	Mass Range Parameters
	Parameter	Value
	CE	30.000
	CES	20.000
	DP	60.000
	IDIx	0.000
	IDUx	5.000
	IRD	66.633
	IRDx	15000.000
	IRW	24.917
	IRWx	10000.000
	IWIx	0.000
	IWUx	5.000
	XA1	71.920

	Start Mass:	50.0
	End Mass:	1200.0

	RF Transmission
	Mass	Time (%)
	40.0	50.0
	120.0	50.0

Period 1, Experiment 14
	Experiment Type:	TOF MS^2
	Num. Cycles:	1684
	Polarity:	Positive
	Product :	IDA
	Period Cycle Time:	2450 ms
	Pulser Frequency:	16.906 kHz
	Accumulation Time:	150.0 ms

	Experiment Parameters
	Parameter	Value
	CUR	30.000
	GS1	40.000
	GS2	45.000
	ISVF	5500.000
	TEM	450.000

	Mass Range Parameters
	Parameter	Value
	CE	30.000
	CES	20.000
	DP	60.000
	IDIx	0.000
	IDUx	5.000
	IRD	66.633
	IRDx	15000.000
	IRW	24.917
	IRWx	10000.000
	IWIx	0.000
	IWUx	5.000
	XA1	72.080

	Start Mass:	50.0
	End Mass:	1200.0

	RF Transmission
	Mass	Time (%)
	40.0	50.0
	120.0	50.0

Period 1, Experiment 15
	Experiment Type:	TOF MS^2
	Num. Cycles:	1684
	Polarity:	Positive
	Product :	IDA
	Period Cycle Time:	2450 ms
	Pulser Frequency:	16.906 kHz
	Accumulation Time:	150.0 ms

	Experiment Parameters
	Parameter	Value
	CUR	30.000
	GS1	40.000
	GS2	45.000
	ISVF	5500.000
	TEM	450.000

	Mass Range Parameters
	Parameter	Value
	CE	30.000
	CES	20.000
	DP	60.000
	IDIx	0.000
	IDUx	5.000
	IRD	66.633
	IRDx	15000.000
	IRW	24.917
	IRWx	10000.000
	IWIx	0.000
	IWUx	5.000
	XA1	72.240

	Start Mass:	50.0
	End Mass:	1200.0

	RF Transmission
	Mass	Time (%)
	40.0	50.0
	120.0	50.0

Period 1, Experiment 16
	Experiment Type:	TOF MS^2
	Num. Cycles:	1684
	Polarity:	Positive
	Product :	IDA
	Period Cycle Time:	2450 ms
	Pulser Frequency:	16.906 kHz
	Accumulation Time:	150.0 ms

	Experiment Parameters
	Parameter	Value
	CUR	30.000
	GS1	40.000
	GS2	45.000
	ISVF	5500.000
	TEM	450.000

	Mass Range Parameters
	Parameter	Value
	CE	30.000
	CES	20.000
	DP	60.000
	IDIx	0.000
	IDUx	5.000
	IRD	66.633
	IRDx	15000.000
	IRW	24.917
	IRWx	10000.000
	IWIx	0.000
	IWUx	5.000
	XA1	72.400

	Start Mass:	50.0
	End Mass:	1200.0

	RF Transmission
	Mass	Time (%)
	40.0	50.0
	120.0	50.0

Instrument Tables and Parameters
	Resolution Table, Quad 1, Positive, Unit, TOF Resolution Mode: High Resolution
	Last Modification Date Time:	February 05, 2015 13:05:44
	IE1:	2.000
	VS1:	-0.315
	HST:	0.000
	VS2:	0.000

	Mass (Da)	Offset Value
	59.049	61.120
	175.133	61.320
	442.337	61.364
	674.505	61.370
	906.672	61.374
	1196.882	61.374

	Resolution Table, Quad 1, Positive, Unit, TOF Resolution Mode: High Sensitivity
	Last Modification Date Time:	February 04, 2015 17:39:47
	IE1:	2.000
	VS1:	-0.079
	HST:	-0.033
	VS2:	0.000

	Mass (Da)	Offset Value
	59.049	61.120
	175.133	61.320
	442.337	61.364
	674.505	61.370
	906.672	61.374
	1196.882	61.374

	Mass Calibration Table, Quad 1, Positive, Unit Resolution
	Last Modification Date Time:	February 03, 2015 13:48:54

	Mass (Da)	Dac Value
	59.049	2493
	175.133	7447
	442.337	18857
	674.505	28773
	906.672	38687
	1196.882	51080

	TOF Mass Calibration Parameters
	Polarity	Scan	Slope	Delay (nsec)
	Positive	TOFMS	7.023514447131617300e-004	-1.628877123933920600e+000
	Positive	MS/MS High Resolution	7.024170131098070700e-004	-1.554330913559251300e+000
	Positive	MS/MS High Sensitivity	7.023718951490750100e-004	-1.659343250300998400e+000
	Negative	TOFMS	7.024378383317660800e-004	-1.575169759773060700e+000
	Negative	MS/MS High Resolution	7.024825380248355500e-004	-1.522020601767653900e+000
	Negative	MS/MS High Sensitivity	7.024524502643503300e-004	-1.274719079614420300e+000

	Show TOF Resolution Parameters in Manual Tune:	No

	Keyed Text
	File was created with the software version:	Analyst TF 1.7

Shimadzu Integrated System
	000 mL/min	
	Pump B Conc:	40.0 %
	B Curve:	0
	Pressure Range (Pump A/B):	0 - 18000 psi
	Pump A Compressability:	Off
	Pump B Compressability:	Off

	Autosampler
	Model:	SIL-30AC
	Use Autosampler:	Yes
	Rinsing Volume:	500 uL
	Needle Stroke:	52 mm.
	Rinsing Speed:	35 uL/sec
	Sampling Speed:	1.0 uL/sec
	Rinse Dip Time:	0 sec
	Rinse Mode:	Before and after aspiration
	Cooler Enabled:	Yes
	Cooler Temperature:	10 deg. C
	Control Vial Needle Stroke:	52 mm
	Rinse Method:	Rinse Port Only
	Rinse Time:	2 sec
	Measuring Line Purge Volume:	100 uL
	Discharge Speed:	1.0 uL/sec
	Air Gap:	Off
	Rinse Port Liquid Selection:	R1
	Rinsing Type:	External
	Purge Time of Rinse Port R0:	10.0 min
	Purge Time of Measuring Line R0:	10.0 min
	Rinsing Start Time Mode:	After Acquisition
	Rinsing Sequence 1:	none
	Rinsing Sequence 2:	none
	Rinsing Sequence 3:	none
	Rinsing Sequence 4:	none
	Rinsing Volume (R0 R1 R2):	300 uL
	Injection Port Rinsing With R0:	On
	Injection Port Rinsing With R1:	On
	Injection Port Rinsing With R2:	On
	Sample Loop Equilibration:	Off

	Oven
	Model:	CTO-30A
	Temperature Control:	Enabled
	Temperature:	55 deg. C
	Max. Temperature:	160 deg. C
	Heat Compensation:	Auto
	Heat Compensation Flow:	0.4000 mL\min

	System Controller
	Model:	CBM-20A
	Power:	On
	Event 1:	Off
	Event 2:	Off
	Event 3:	Off
	Event 4:	Off

	Time Program
	Time	Module	Events	Parameter
	0.01	Pumps	Pump B Conc.	40
	10.00	Pumps	Pump B Conc.	98
	17.00	Pumps	Pump B Conc.	98
	17.10	Pumps	Pump B Conc.	40
	20.00	System Controller	Stop	

	Pretreatment
	Mode:	Standard
		

