Acquisition Info
	Method Path:	D:\Analyst Data\Projects\TM_Methods\Acquisition Methods\LIP_15min_MSMS_Jenny_New_20min_Negative.dam
	Batch Path:	D:\Analyst Data\Projects\TM_Methods\Batch\20150414_Ring_Trial_Negative.dab
	Acquisition Date:	Wednesday, April 15, 2015
	Acquisition Time:	5:45:00 AM
	User Name:	ABI_5600
	Acquisition Method:	LIP_15min_MSMS_Jenny_New_20min_Negative.dam
	Rack:	1
	Plate:	1
	Vial:	3
	Injection Volume:	5.00

Quantitation Info
	Sample Type:	Unknown
	Dilution Factor:	1.0000

Log Info
	Integrated System:	Shimadzu Controller,  CBM20A
	Serial#:	L20235154646
	ROM Version:	2.40
	Pressure Units:	psi
	Pump:	Shimadzu LC30AD
	Serial#:	L20555151103
	ROM Version:	3.00
	Pump:	Shimadzu LC30AD
	Serial#:	L20555151046
	ROM Version:	3.00
	AutoSampler:	Shimadzu SIL30AC
	Serial#:	L20565250433
	ROM Version:	3.10
	Column Oven:	Shimadzu CTO30A
	Serial#:	L20575150303
	ROM Version:	3.00
	Injection Volume used:	5.00 µl
	Mass Spectrometer:	TripleTOF 5600-1
	Config Table Version:	02
	Firmware Version:	MIA3000 ------- MIL4000 MIB3000
	Component Name:	Hybrid Quadrupole-TOF LC/MS/MS Mass Spectrometer
	Component ID:	TripleTOF 5600+
	Manufacturer:	AB Sciex Instruments
	Model:	5016230/L
	Serial Number:	BN20031205H
	Source Housing:	DuoSpray Ion Source
	Static Accumulation.:	N/A

	Time (from start of run):	00:00:01
	Mass Spectrometer:	TripleTOF 5600-1
	Start of Run - Detailed Status:	N/A
	Vacuum Status:	At Pressure
	Vacuum Gauge (10e-5 Torr):	3.6
	Backing Pump:	Ok
	Q1 Turbo Pump:	Normal
	Q2/TOF Turbo Pump:	Normal
	Sample Introduction Status:	Ready
	Source/Ion Path Electronics:	On
	Source Type:	DuoSpray Ion Source
	Source Temperature (at setpoint):	450.0 C
	Source Exhaust Pump:	Ok
	Injection Manifold:	Bypass

	Time (from start of run):	00:20:35
	Mass Spectrometer:	TripleTOF 5600-1
	End of Run - Detailed Status:	N/A
	Vacuum Status:	At Pressure
	Vacuum Gauge (10e-5 Torr):	3.6
	Backing Pump:	Ok
	Q1 Turbo Pump:	Normal
	Q2/TOF Turbo Pump:	Normal
	Sample Introduction Status:	Ready
	Source/Ion Path Electronics:	On
	Source Type:	DuoSpray Ion Source
	Source Temperature (at setpoint):	450.0 C
	Source Exhaust Pump:	Ok
	Injection Manifold:	Bypass

IDA
	With intensity greater than:	300
	Switch after:	15 spectra
	Use advanced settings:	True
	Always exclude:	True
	Exclude for:	9 sec
	Mass tolerance units:	mDa
	Mass tolerance:	50
	Use inclusion list:	False
	Use exclusion list:	False
	Ignore peaks within:	6 Da
	Real time:	None
	Spectrum file:	aaa
	Dynamic background subtraction:	True
	Fragment intensity multiplier:	2
	Maximum accumulation:	2 sec
	Allow standard filters for Smart IDA:	True
	Never exclude former target ions:	True
	Exclude after occurences:	1

Period 1, Experiment 1
	Experiment Type:	TOF MS
	Num. Cycles:	2164
	Polarity:	Negative
	Period Cycle Time:	2450 ms
	Pulser Frequency:	16.906 kHz
	Accumulation Time:	150.0 ms

	Experiment Parameters
	Parameter	Value
	CUR	30.000
	GS1	40.000
	GS2	45.000
	ISVF	4500.000
	TEM	450.000

	Mass Range Parameters
	Parameter	Value
	CE	-10.000
	DP	-60.000
	IDIx	0.000
	IDUx	5.000
	IRDx	15000.000
	IRWx	10000.000
	IWIx	0.000
	IWUx	5.000
	XA1	139.530

	Start Mass:	50.0
	End Mass:	1200.0

	RF Transmission
	Mass	Time (%)
	40.0	33.0
	120.0	33.0
	390.0	34.0

Period 1, Experiment 2
	Experiment Type:	TOF MS^2
	Num. Cycles:	2164
	Polarity:	Negative
	Product :	IDA
	Period Cycle Time:	2450 ms
	Pulser Frequency:	16.906 kHz
	Accumulation Time:	150.0 ms

	Experiment Parameters
	Parameter	Value
	CUR	30.000
	GS1	40.000
	GS2	45.000
	ISVF	4500.000
	TEM	450.000

	Mass Range Parameters
	Parameter	Value
	CE	-30.000
	CES	20.000
	DP	-60.000
	IDIx	0.000
	IDUx	5.000
	IRD	66.633
	IRDx	15000.000
	IRW	24.917
	IRWx	10000.000
	IWIx	0.000
	IWUx	5.000
	XA1	70.160

	Start Mass:	50.0
	End Mass:	1200.0

	RF Transmission
	Mass	Time (%)
	40.0	50.0
	120.0	50.0

Period 1, Experiment 3
	Experiment Type:	TOF MS^2
	Num. Cycles:	2164
	Polarity:	Negative
	Product :	IDA
	Period Cycle Time:	2450 ms
	Pulser Frequency:	16.906 kHz
	Accumulation Time:	150.0 ms

	Experiment Parameters
	Parameter	Value
	CUR	30.000
	GS1	40.000
	GS2	45.000
	ISVF	4500.000
	TEM	450.000

	Mass Range Parameters
	Parameter	Value
	CE	-30.000
	CES	20.000
	DP	-60.000
	IDIx	0.000
	IDUx	5.000
	IRD	66.633
	IRDx	15000.000
	IRW	24.917
	IRWx	10000.000
	IWIx	0.000
	IWUx	5.000
	XA1	70.320

	Start Mass:	50.0
	End Mass:	1200.0

	RF Transmission
	Mass	Time (%)
	40.0	50.0
	120.0	50.0

Period 1, Experiment 4
	Experiment Type:	TOF MS^2
	Num. Cycles:	2164
	Polarity:	Negative
	Product :	IDA
	Period Cycle Time:	2450 ms
	Pulser Frequency:	16.906 kHz
	Accumulation Time:	150.0 ms

	Experiment Parameters
	Parameter	Value
	CUR	30.000
	GS1	40.000
	GS2	45.000
	ISVF	4500.000
	TEM	450.000

	Mass Range Parameters
	Parameter	Value
	CE	-30.000
	CES	20.000
	DP	-60.000
	IDIx	0.000
	IDUx	5.000
	IRD	66.633
	IRDx	15000.000
	IRW	24.917
	IRWx	10000.000
	IWIx	0.000
	IWUx	5.000
	XA1	70.480

	Start Mass:	50.0
	End Mass:	1200.0

	RF Transmission
	Mass	Time (%)
	40.0	50.0
	120.0	50.0

Period 1, Experiment 5
	Experiment Type:	TOF MS^2
	Num. Cycles:	2164
	Polarity:	Negative
	Product :	IDA
	Period Cycle Time:	2450 ms
	Pulser Frequency:	16.906 kHz
	Accumulation Time:	150.0 ms

	Experiment Parameters
	Parameter	Value
	CUR	30.000
	GS1	40.000
	GS2	45.000
	ISVF	4500.000
	TEM	450.000

	Mass Range Parameters
	Parameter	Value
	CE	-30.000
	CES	20.000
	DP	-60.000
	IDIx	0.000
	IDUx	5.000
	IRD	66.633
	IRDx	15000.000
	IRW	24.917
	IRWx	10000.000
	IWIx	0.000
	IWUx	5.000
	XA1	70.640

	Start Mass:	50.0
	End Mass:	1200.0

	RF Transmission
	Mass	Time (%)
	40.0	50.0
	120.0	50.0

Period 1, Experiment 6
	Experiment Type:	TOF MS^2
	Num. Cycles:	2164
	Polarity:	Negative
	Product :	IDA
	Period Cycle Time:	2450 ms
	Pulser Frequency:	16.906 kHz
	Accumulation Time:	150.0 ms

	Experiment Parameters
	Parameter	Value
	CUR	30.000
	GS1	40.000
	GS2	45.000
	ISVF	4500.000
	TEM	450.000

	Mass Range Parameters
	Parameter	Value
	CE	-30.000
	CES	20.000
	DP	-60.000
	IDIx	0.000
	IDUx	5.000
	IRD	66.633
	IRDx	15000.000
	IRW	24.917
	IRWx	10000.000
	IWIx	0.000
	IWUx	5.000
	XA1	70.800

	Start Mass:	50.0
	End Mass:	1200.0

	RF Transmission
	Mass	Time (%)
	40.0	50.0
	120.0	50.0

Period 1, Experiment 7
	Experiment Type:	TOF MS^2
	Num. Cycles:	2164
	Polarity:	Negative
	Product :	IDA
	Period Cycle Time:	2450 ms
	Pulser Frequency:	16.906 kHz
	Accumulation Time:	150.0 ms

	Experiment Parameters
	Parameter	Value
	CUR	30.000
	GS1	40.000
	GS2	45.000
	ISVF	4500.000
	TEM	450.000

	Mass Range Parameters
	Parameter	Value
	CE	-30.000
	CES	20.000
	DP	-60.000
	IDIx	0.000
	IDUx	5.000
	IRD	66.633
	IRDx	15000.000
	IRW	24.917
	IRWx	10000.000
	IWIx	0.000
	IWUx	5.000
	XA1	70.960

	Start Mass:	50.0
	End Mass:	1200.0

	RF Transmission
	Mass	Time (%)
	40.0	50.0
	120.0	50.0

Period 1, Experiment 8
	Experiment Type:	TOF MS^2
	Num. Cycles:	2164
	Polarity:	Negative
	Product :	IDA
	Period Cycle Time:	2450 ms
	Pulser Frequency:	16.906 kHz
	Accumulation Time:	150.0 ms

	Experiment Parameters
	Parameter	Value
	CUR	30.000
	GS1	40.000
	GS2	45.000
	ISVF	4500.000
	TEM	450.000

	Mass Range Parameters
	Parameter	Value
	CE	-30.000
	CES	20.000
	DP	-60.000
	IDIx	0.000
	IDUx	5.000
	IRD	66.633
	IRDx	15000.000
	IRW	24.917
	IRWx	10000.000
	IWIx	0.000
	IWUx	5.000
	XA1	71.120

	Start Mass:	50.0
	End Mass:	1200.0

	RF Transmission
	Mass	Time (%)
	40.0	50.0
	120.0	50.0

Period 1, Experiment 9
	Experiment Type:	TOF MS^2
	Num. Cycles:	2164
	Polarity:	Negative
	Product :	IDA
	Period Cycle Time:	2450 ms
	Pulser Frequency:	16.906 kHz
	Accumulation Time:	150.0 ms

	Experiment Parameters
	Parameter	Value
	CUR	30.000
	GS1	40.000
	GS2	45.000
	ISVF	4500.000
	TEM	450.000

	Mass Range Parameters
	Parameter	Value
	CE	-30.000
	CES	20.000
	DP	-60.000
	IDIx	0.000
	IDUx	5.000
	IRD	66.633
	IRDx	15000.000
	IRW	24.917
	IRWx	10000.000
	IWIx	0.000
	IWUx	5.000
	XA1	71.280

	Start Mass:	50.0
	End Mass:	1200.0

	RF Transmission
	Mass	Time (%)
	40.0	50.0
	120.0	50.0

Period 1, Experiment 10
	Experiment Type:	TOF MS^2
	Num. Cycles:	2164
	Polarity:	Negative
	Product :	IDA
	Period Cycle Time:	2450 ms
	Pulser Frequency:	16.906 kHz
	Accumulation Time:	150.0 ms

	Experiment Parameters
	Parameter	Value
	CUR	30.000
	GS1	40.000
	GS2	45.000
	ISVF	4500.000
	TEM	450.000

	Mass Range Parameters
	Parameter	Value
	CE	-30.000
	CES	20.000
	DP	-60.000
	IDIx	0.000
	IDUx	5.000
	IRD	66.633
	IRDx	15000.000
	IRW	24.917
	IRWx	10000.000
	IWIx	0.000
	IWUx	5.000
	XA1	71.440

	Start Mass:	50.0
	End Mass:	1200.0

	RF Transmission
	Mass	Time (%)
	40.0	50.0
	120.0	50.0

Period 1, Experiment 11
	Experiment Type:	TOF MS^2
	Num. Cycles:	2164
	Polarity:	Negative
	Product :	IDA
	Period Cycle Time:	2450 ms
	Pulser Frequency:	16.906 kHz
	Accumulation Time:	150.0 ms

	Experiment Parameters
	Parameter	Value
	CUR	30.000
	GS1	40.000
	GS2	45.000
	ISVF	4500.000
	TEM	450.000

	Mass Range Parameters
	Parameter	Value
	CE	-30.000
	CES	20.000
	DP	-60.000
	IDIx	0.000
	IDUx	5.000
	IRD	66.633
	IRDx	15000.000
	IRW	24.917
	IRWx	10000.000
	IWIx	0.000
	IWUx	5.000
	XA1	71.600

	Start Mass:	50.0
	End Mass:	1200.0

	RF Transmission
	Mass	Time (%)
	40.0	50.0
	120.0	50.0

Period 1, Experiment 12
	Experiment Type:	TOF MS^2
	Num. Cycles:	2164
	Polarity:	Negative
	Product :	IDA
	Period Cycle Time:	2450 ms
	Pulser Frequency:	16.906 kHz
	Accumulation Time:	150.0 ms

	Experiment Parameters
	Parameter	Value
	CUR	30.000
	GS1	40.000
	GS2	45.000
	ISVF	4500.000
	TEM	450.000

	Mass Range Parameters
	Parameter	Value
	CE	-30.000
	CES	20.000
	DP	-60.000
	IDIx	0.000
	IDUx	5.000
	IRD	66.633
	IRDx	15000.000
	IRW	24.917
	IRWx	10000.000
	IWIx	0.000
	IWUx	5.000
	XA1	71.760

	Start Mass:	50.0
	End Mass:	1200.0

	RF Transmission
	Mass	Time (%)
	40.0	50.0
	120.0	50.0

Period 1, Experiment 13
	Experiment Type:	TOF MS^2
	Num. Cycles:	2164
	Polarity:	Negative
	Product :	IDA
	Period Cycle Time:	2450 ms
	Pulser Frequency:	16.906 kHz
	Accumulation Time:	150.0 ms

	Experiment Parameters
	Parameter	Value
	CUR	30.000
	GS1	40.000
	GS2	45.000
	ISVF	4500.000
	TEM	450.000

	Mass Range Parameters
	Parameter	Value
	CE	-30.000
	CES	20.000
	DP	-60.000
	IDIx	0.000
	IDUx	5.000
	IRD	66.633
	IRDx	15000.000
	IRW	24.917
	IRWx	10000.000
	IWIx	0.000
	IWUx	5.000
	XA1	71.920

	Start Mass:	50.0
	End Mass:	1200.0

	RF Transmission
	Mass	Time (%)
	40.0	50.0
	120.0	50.0

Period 1, Experiment 14
	Experiment Type:	TOF MS^2
	Num. Cycles:	2164
	Polarity:	Negative
	Product :	IDA
	Period Cycle Time:	2450 ms
	Pulser Frequency:	16.906 kHz
	Accumulation Time:	150.0 ms

	Experiment Parameters
	Parameter	Value
	CUR	30.000
	GS1	40.000
	GS2	45.000
	ISVF	4500.000
	TEM	450.000

	Mass Range Parameters
	Parameter	Value
	CE	-30.000
	CES	20.000
	DP	-60.000
	IDIx	0.000
	IDUx	5.000
	IRD	66.633
	IRDx	15000.000
	IRW	24.917
	IRWx	10000.000
	IWIx	0.000
	IWUx	5.000
	XA1	72.080

	Start Mass:	50.0
	End Mass:	1200.0

	RF Transmission
	Mass	Time (%)
	40.0	50.0
	120.0	50.0

Period 1, Experiment 15
	Experiment Type:	TOF MS^2
	Num. Cycles:	2164
	Polarity:	Negative
	Product :	IDA
	Period Cycle Time:	2450 ms
	Pulser Frequency:	16.906 kHz
	Accumulation Time:	150.0 ms

	Experiment Parameters
	Parameter	Value
	CUR	30.000
	GS1	40.000
	GS2	45.000
	ISVF	4500.000
	TEM	450.000

	Mass Range Parameters
	Parameter	Value
	CE	-30.000
	CES	20.000
	DP	-60.000
	IDIx	0.000
	IDUx	5.000
	IRD	66.633
	IRDx	15000.000
	IRW	24.917
	IRWx	10000.000
	IWIx	0.000
	IWUx	5.000
	XA1	72.240

	Start Mass:	50.0
	End Mass:	1200.0

	RF Transmission
	Mass	Time (%)
	40.0	50.0
	120.0	50.0

Period 1, Experiment 16
	Experiment Type:	TOF MS^2
	Num. Cycles:	2164
	Polarity:	Negative
	Product :	IDA
	Period Cycle Time:	2450 ms
	Pulser Frequency:	16.906 kHz
	Accumulation Time:	150.0 ms

	Experiment Parameters
	Parameter	Value
	CUR	30.000
	GS1	40.000
	GS2	45.000
	ISVF	4500.000
	TEM	450.000

	Mass Range Parameters
	Parameter	Value
	CE	-30.000
	CES	20.000
	DP	-60.000
	IDIx	0.000
	IDUx	5.000
	IRD	66.633
	IRDx	15000.000
	IRW	24.917
	IRWx	10000.000
	IWIx	0.000
	IWUx	5.000
	XA1	72.400

	Start Mass:	50.0
	End Mass:	1200.0

	RF Transmission
	Mass	Time (%)
	40.0	50.0
	120.0	50.0

Instrument Tables and Parameters
	Resolution Table, Quad 1, Negative, Unit, TOF Resolution Mode: High Resolution
	Last Modification Date Time:	February 05, 2015 12:44:28
	IE1:	-2.000
	VS1:	0.320
	HST:	0.000
	VS2:	0.000

	Mass (Da)	Offset Value
	44.998	60.992
	411.260	61.339
	585.385	61.355
	933.637	61.367
	1165.804	61.369

	Resolution Table, Quad 1, Negative, Unit, TOF Resolution Mode: High Sensitivity
	Last Modification Date Time:	February 04, 2015 15:03:44
	IE1:	-2.100
	VS1:	0.081
	HST:	1.081
	VS2:	0.000

	Mass (Da)	Offset Value
	44.998	60.992
	411.260	61.339
	585.385	61.355
	933.637	61.367
	1165.804	61.369

	Mass Calibration Table, Quad 1, Negative, Unit Resolution
	Last Modification Date Time:	February 03, 2015 13:52:00

	Mass (Da)	Dac Value
	44.998	1892
	411.260	17529
	585.385	24966
	933.637	39837
	1165.804	49753

	TOF Mass Calibration Parameters
	Polarity	Scan	Slope	Delay (nsec)
	Positive	TOFMS	7.023599387177949600e-004	-1.487841349949707400e+000
	Positive	MS/MS High Resolution	7.024170131098070700e-004	-1.554330913559251300e+000
	Positive	MS/MS High Sensitivity	7.023740613101379200e-004	-1.543015407916732600e+000
	Negative	TOFMS	7.024389470828960500e-004	-1.637017723928669000e+000
	Negative	MS/MS High Resolution	7.024825380248355500e-004	-1.522020601767653900e+000
	Negative	MS/MS High Sensitivity	7.024547937958907100e-004	-1.302273609853555200e+000

	Show TOF Resolution Parameters in Manual Tune:	No

	Keyed Text
	File was created with the software version:	Analyst TF 1.7

Shimadzu Integrated System
	000 mL/min	
	Pump B Conc:	40.0 %
	B Curve:	0
	Pressure Range (Pump A/B):	0 - 18000 psi
	Pump A Compressability:	Off
	Pump B Compressability:	Off

	Autosampler
	Model:	SIL-30AC
	Use Autosampler:	Yes
	Rinsing Volume:	500 uL
	Needle Stroke:	52 mm.
	Rinsing Speed:	35 uL/sec
	Sampling Speed:	1.0 uL/sec
	Rinse Dip Time:	0 sec
	Rinse Mode:	Before and after aspiration
	Cooler Enabled:	Yes
	Cooler Temperature:	10 deg. C
	Control Vial Needle Stroke:	52 mm
	Rinse Method:	Rinse Port Only
	Rinse Time:	2 sec
	Measuring Line Purge Volume:	100 uL
	Discharge Speed:	1.0 uL/sec
	Air Gap:	Off
	Rinse Port Liquid Selection:	R1
	Rinsing Type:	External
	Purge Time of Rinse Port R0:	10.0 min
	Purge Time of Measuring Line R0:	10.0 min
	Rinsing Start Time Mode:	After Acquisition
	Rinsing Sequence 1:	none
	Rinsing Sequence 2:	none
	Rinsing Sequence 3:	none
	Rinsing Sequence 4:	none
	Rinsing Volume (R0 R1 R2):	300 uL
	Injection Port Rinsing With R0:	On
	Injection Port Rinsing With R1:	On
	Injection Port Rinsing With R2:	On
	Sample Loop Equilibration:	Off

	Oven
	Model:	CTO-30A
	Temperature Control:	Enabled
	Temperature:	55 deg. C
	Max. Temperature:	160 deg. C
	Heat Compensation:	Auto
	Heat Compensation Flow:	0.4000 mL\min

	System Controller
	Model:	CBM-20A
	Power:	On
	Event 1:	Off
	Event 2:	Off
	Event 3:	Off
	Event 4:	Off

	Time Program
	Time	Module	Events	Parameter
	0.01	Pumps	Pump B Conc.	40
	10.00	Pumps	Pump B Conc.	98
	17.00	Pumps	Pump B Conc.	98
	17.10	Pumps	Pump B Conc.	40
	20.00	System Controller	Stop	

	Pretreatment
	Mode:	Standard
		

