#METABOLOMICS WORKBENCH TEXT OUTPUT STUDY_ID:	ANALYSIS_ID:	
VERSION             	1
CREATED_ON          	2017-06-17
#PROJECT
PR:PROJECT_TITLE    	shACADM Lipidomics
PR:PROJECT_TYPE     	MS analysis
PR:PROJECT_SUMMARY  	Looking for changes in lipid levels with knock out of ACADM
PR:INSTITUTE        	University of Michigan
PR:DEPARTMENT       	Molecular and Integrative Physiology
PR:LABORATORY       	Lyssiotis Lab
PR:LAST_NAME        	Lyssiotis
PR:FIRST_NAME       	Costas
PR:ADDRESS          	Ann Arbor, MI
PR:EMAIL            	clyssiot@umich.edu
PR:PHONE            	734-615-9133
PR:FUNDING_SOURCE   	
PR:PROJECT_COMMENTS 	
PR:PUBLICATIONS     	
#STUDY
ST:STUDY_TITLE      	Looking for changes in lipid levels with knock out of ACADM (shACADM)
ST:STUDY_TYPE       	MS analysis
ST:STUDY_SUMMARY    	GSC 8-11 cells (20 million) were transduced with lentivirus coding for shACADM 4, 6, or scr (control). ACADM is an enzyme responsible for the metabolism of medium chain fatty acids (C4-C12). A round of selection was performed using puromycin to ensure only cells that had been transduced survived. Knockdown of ACADM was confirmed via western blot. Pellets were collected by centrifuging cells for 10 min at max speed. The pellets were washed and each sample was split into quadruplicate.
ST:INSTITUTE        	University of Michigan
ST:DEPARTMENT       	Biomedical Research Core Facilities
ST:LABORATORY       	Metabolomics core
ST:LAST_NAME        	Kachman
ST:FIRST_NAME       	Maureen
ST:ADDRESS          	6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
ST:EMAIL            	mkachman@med.umich.edu
ST:PHONE            	(734) 232-8175
ST:SUBMIT_DATE      	03/17/2016 02:51:22 PM
ST:NUM_GROUPS       	3
ST:TOTAL_SUBJECTS   	3
ST:STUDY_COMMENTS   	
#SUBJECT_SAMPLE_FACTORS:          	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data
SUBJECT_SAMPLE_FACTORS            	SU0014234	S00021731	Transduced with: shACADM4	
SUBJECT_SAMPLE_FACTORS            	SU0014234	S00021732	Transduced with: shACADM4	
SUBJECT_SAMPLE_FACTORS            	SU0014234	S00021733	Transduced with: shACADM4	
SUBJECT_SAMPLE_FACTORS            	SU0014234	S00021734	Transduced with: shACADM4	
SUBJECT_SAMPLE_FACTORS            	SU0014235	S00021735	Transduced with: shACADM6	
SUBJECT_SAMPLE_FACTORS            	SU0014235	S00021736	Transduced with: shACADM6	
SUBJECT_SAMPLE_FACTORS            	SU0014235	S00021737	Transduced with: shACADM6	
SUBJECT_SAMPLE_FACTORS            	SU0014235	S00021738	Transduced with: shACADM6	
SUBJECT_SAMPLE_FACTORS            	SU0014236	S00021739	Transduced with: shACADMscr	
SUBJECT_SAMPLE_FACTORS            	SU0014236	S00021740	Transduced with: shACADMscr	
SUBJECT_SAMPLE_FACTORS            	SU0014236	S00021741	Transduced with: shACADMscr	
SUBJECT_SAMPLE_FACTORS            	SU0014236	S00021742	Transduced with: shACADMscr	
#SUBJECT
SU:SUBJECT_TYPE                   	HUMAN
SU:SUBJECT_SPECIES                	Homo sapiens
SU:TAXONOMY_ID                    	9606
#COLLECTION
CO:COLLECTION_SUMMARY             	-
#TREATMENT
TR:TREATMENT_SUMMARY              	-
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY             	-
SP:SAMPLEPREP_PROTOCOL_FILENAME   	A008 - Acyl Carnitines.pdf
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_TYPE            	
CH:INSTRUMENT_NAME                	6490A QQQ LC/MS (Agilent)
CH:COLUMN_NAME                    	 ()
CH:METHODS_FILENAME               	QM-004-Xbridg2mm_ACar+_MRM-Insert-gradtest3RC20150731
#ANALYSIS
AN:ANALYSIS_TYPE                  	MRM
AN:INSTRUMENT_NAME                	6490A QQQ LC/MS (Agilent)
AN:ANALYSIS_PROTOCOL_FILE         	A008 - Acyl Carnitines.pdf
AN:ACQUISITION_PARAMETERS_FILE    	QM-004-Xbridg2mm_ACar+_MRM-Insert-gradtest3RC20150731
AN:PROCESSING_PARAMETERS_FILE     	-
#MS
MS:INSTRUMENT_TYPE                	QQQ
MS:MS_TYPE                        	
MS:ION_MODE                       	POSITIVE
#MS_METABOLITE_DATA
MS_METABOLITE_DATA:UNITS	pmol/ug protein
MS_METABOLITE_DATA_START
Sample	S00021731	S00021732	S00021733	S00021734	S00021735	S00021736	S00021737	S00021738	S00021739	S00021740	S00021741	S00021742
Factors	Transduced with: shACADM4	Transduced with: shACADM4	Transduced with: shACADM4	Transduced with: shACADM4	Transduced with: shACADM6	Transduced with: shACADM6	Transduced with: shACADM6	Transduced with: shACADM6	Transduced with: shACADMscr	Transduced with: shACADMscr	Transduced with: shACADMscr	Transduced with: shACADMscr
ACETYL-DL-CARNITINE	15.20546067	18.07056182	11.24574751	11.99804636	12.28310183	14.26191246	13.37886282	14.17667391	14.88614288	13.55534447	15.24164069	16.65295116
DECANOYLCARNITINE	.019825831	.023556327	.017296331	.015469811	.019865695	.023137137	.029889508	.027207317	.032477726	.026342887	.026964221	.033380675
LAUROYLCARNITINE	.076009032	.079841127	.063232398	.057745946	.061003535	.077116223	.099731885	.115633126	.104729034	.07721505	.083590866	.110709395
L-PALMITOYLCARNITINE	1.652673647	2.116380703	1.558703454	1.343585015	1.264035074	2.118303482	1.96669206	2.044392507	2.950571025	2.220557337	2.681129048	3.544717052
O-OCTANOYLCARNITINE	.029410786	.035269988	.025815132	.02362421	.026478902	.02914819	.035556273	.034548634	.034140647	.034516108	.029422466	.036698479
BUTYRYLCARNITINE	4.198895116	5.97455274	1.736132372	1.925018177	1.773224924	3.997991689	3.752709994	4.276347313	2.123717138	1.996315714	2.982466414	4.422392566
DL-CARNITINE	39.04638787	35.90193865	28.62596415	22.69878065	25.66590397	28.05625304	35.57130629	28.54777973	26.38395205	27.70516827	26.51128093	43.23183794
AC1MIY4G	.223359713	.235742671	.152971439	.156842788	.149714063	.170986063	.173926083	.200902628	.17339216	.201690186	.130788128	.197626479
ARACHIDONYL CARNITINE	.178152598	.285635074	.201952603	.165244515	.117392046	.257568796	.288207225	.231830006	.538386682	.297795456	.397421743	.547055516
ARACHIDYL CARNITINE	.001262277	.001838382	.001392142	.000993326	.001924772	.00190596	.002735157	.001880514	.004201177	.00522658	.003439934	.004260206
LINOELAIDYL CARNITINE	.284375381	.444721223	.315570023	.255897991	.268474417	.447359409	.475507873	.48480516	.708065132	.438848592	.552463604	.779834283
18:3 CARNITINE	.122876233	.23643141	.14472722	.121376077	.148936081	.230763844	.270103852	.257197543	.461766132	.294976408	.379355094	.42267782
CIS-4-DECENOYL CARNITINE	.000900918	.001158032	.000956743	.000955765	.000717847	.00098573	.001489242	.001174315	.001386959	.001404463	.001453604	.001499192
ISOVALERYLCARNITINE	2.652254125	2.937415265	2.117797375	2.004763588	1.860942903	2.417168977	2.502310528	2.49530889	2.170679638	2.264237426	1.980162821	2.505542768
MYRISTOYLCARNITINE	.588138591	.780201822	.672898195	.490404598	.400745385	.578588376	.793121634	.823334224	1.29512326	.899480792	.998553208	1.33046501
STEAROYLCARNITINE	.156742723	.237115312	.140397438	.154720961	.195263194	.273613444	.284990738	.288709887	.572525419	.346542636	.693505162	.639366341
OLEOYLCARNITINE	.96764442	1.358441396	1.040343445	.936244733	1.174851185	1.941606779	2.037052701	2.004514605	2.73733414	2.033944686	2.534700612	3.115771646
GLUTARYLCARNITINE	.030756535	.034013945	.023240727	.022134714	.023577981	.029096595	.027767981	.032645462	.02437852	.024295223	.023508632	.027784331
11-CIS-ICOSENEOYLCARNITINE	.009066514	.015546614	.009366663	.00803587	.014136043	.017048793	.020769615	.022517112	.035595748	.02144162	.040072832	.038860346
(11Z,14Z)-ICOSADIENOYLCARNITINE	.040319131	.065835285	.049416112	.043469882	.060185672	.094802412	.102017102	.099171672	.189938176	.112813376	.187150516	.227406054
3-HYDROXYLAUROYLCARNITINE	.00638321	.006719879	.005990168	.004816496	.005306895	.006180265	.008422667	.0090673	.015488324	.008961206	.013576891	.016789917
3-HYDROXYMYRISTOYLCARNITINE	.014853983	.017344924	.013999634	.010885017	.007930294	.008740426	.010724994	.013502193	.021963737	.016019515	.02138328	.025181908
3-HYDROXYPALMITOYLCARNITINE	.031141264	.038381422	.034258577	.027028691	.032376225	.044669943	.04910566	.049214047	.066844467	.04832983	.056728447	.070087951
3-HYDROXYLINOLEYLCARNITINE	.009090149	.011036852	.010030736	.008849648	.010700174	.018475314	.023838276	.020362177	.039894842	.018842113	.039443391	.048301783
16:1 CARNITINE	.483084065	.688101889	.494762955	.419472318	.616721529	.944364128	1.002222948	1.019592279	1.83798215	1.278923267	1.600873438	1.940727011
8:1 CARNITINE	.007483505	.006849324	.006784219	.00620255	.006308905	.007256629	.008469802	.008592152	.007122784	.006198747	.005889251	.007274132
14:2 CARNITINE	.033464124	.043729134	.04364662	.032244228	.033680233	.043864069	.066290765	.068443206	.07928557	.052135792	.063624376	.070751383
12:1 CARNITINE	.011001841	.013126047	.011665139	.009820332	.013256599	.015920587	.024833216	.02597028	.030529773	.019342797	.030556723	.032240898
14:1 CARNITINE	.133452099	.192421467	.17530569	.141625931	.176187928	.259304473	.362029268	.335685018	.583522222	.345251589	.464835723	.482767334
MS_METABOLITE_DATA_END
#METABOLITES
METABOLITES_START
metabolite_name	moverz_quant	ri	ri_type	pubchem_id	inchi_key	kegg_id	other_id	other_id_type
ACETYL-DL-CARNITINE				1				
DECANOYLCARNITINE				10245190				
LAUROYLCARNITINE				10427569				
L-PALMITOYLCARNITINE				11953816				
O-OCTANOYLCARNITINE				123701				
BUTYRYLCARNITINE				213144				
DL-CARNITINE				288				
AC1MIY4G				3082079				
ARACHIDONYL CARNITINE				53477832				
ARACHIDYL CARNITINE				53477833				
LINOELAIDYL CARNITINE				53477834				
18:3 CARNITINE				54500892				
CIS-4-DECENOYL CARNITINE				57357170				
ISOVALERYLCARNITINE				6426851				
MYRISTOYLCARNITINE				6426854				
STEAROYLCARNITINE				6426855				
OLEOYLCARNITINE				6441392				
GLUTARYLCARNITINE				71317118				
11-CIS-ICOSENEOYLCARNITINE				71464507				
(11Z,14Z)-ICOSADIENOYLCARNITINE				71464509				
3-HYDROXYLAUROYLCARNITINE				71464535				
3-HYDROXYMYRISTOYLCARNITINE				71464541				
3-HYDROXYPALMITOYLCARNITINE				71464553				
3-HYDROXYLINOLEYLCARNITINE				71464556				
16:1 CARNITINE				76040365				
8:1 CARNITINE				77908888				
14:2 CARNITINE				87087940				
12:1 CARNITINE				87087953				
14:1 CARNITINE				87088002				
METABOLITES_END
