#METABOLOMICS WORKBENCH TEXT OUTPUT STUDY_ID:	ANALYSIS_ID:	
VERSION             	1
CREATED_ON          	2017-08-01
#PROJECT
PR:PROJECT_TITLE    	Metabolic regulation by PARP-1 in myofibroblast differentiation and fibrosis
PR:PROJECT_TYPE     	MS analysis
PR:PROJECT_SUMMARY  	Analysis of wild type lung tissue after saline or bleomycin treatment
PR:INSTITUTE        	University of Michigan
PR:DEPARTMENT       	Deaprtment of Pathology
PR:LABORATORY       	Sem H. Phan
PR:LAST_NAME        	Hu
PR:FIRST_NAME       	Biao
PR:ADDRESS          	Ann Arbor, MI
PR:EMAIL            	biaohu@med.umich.edu
PR:PHONE            	734-7635731
PR:FUNDING_SOURCE   	
PR:PROJECT_COMMENTS 	
PR:PUBLICATIONS     	
#STUDY
ST:STUDY_TITLE      	Metabolic analysis of Parp1 ko/wt Saline & Bleo Mouse Lung Fiboblasts and Human IPF & Normal Lung Fiboblasts 3
ST:STUDY_TYPE       	MS analysis
ST:STUDY_SUMMARY    	Glycolysis/TCA/Nucleotide analysis (especially interested in alpha-ketoglutarate) and NAD+ and related metabolite analysis for all samples
ST:INSTITUTE        	University of Michigan
ST:DEPARTMENT       	Biomedical Research Core Facilities
ST:LABORATORY       	Metabolomics core
ST:LAST_NAME        	Kachman
ST:FIRST_NAME       	Maureen
ST:ADDRESS          	6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
ST:EMAIL            	mkachman@med.umich.edu
ST:PHONE            	(734) 232-8175
ST:SUBMIT_DATE      	08/08/2014 02:01:21 PM
ST:NUM_GROUPS       	6
ST:TOTAL_SUBJECTS   	16
ST:STUDY_COMMENTS   	
#SUBJECT
SU:SUBJECT_TYPE                   	HUMAN
SU:SUBJECT_SPECIES                	Homo sapiens
SU:TAXONOMY_ID                    	9606
#SUBJECT_SAMPLE_FACTORS:          	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data
SUBJECT_SAMPLE_FACTORS            	SU0004492	S00015469	Sample type:Tissues | Species:Mus musculus | bleomycin/IPF:N | saline/HLF:Y | wt B6:Y	
SUBJECT_SAMPLE_FACTORS            	SU0004493	S00015470	Sample type:Tissues | Species:Mus musculus | bleomycin/IPF:N | saline/HLF:Y | wt B6:Y	
SUBJECT_SAMPLE_FACTORS            	SU0004494	S00015471	Sample type:Tissues | Species:Mus musculus | bleomycin/IPF:Y | saline/HLF:N | wt B6:Y	
SUBJECT_SAMPLE_FACTORS            	SU0004495	S00015472	Sample type:Tissues | Species:Mus musculus | bleomycin/IPF:Y | saline/HLF:N | wt B6:Y	
SUBJECT_SAMPLE_FACTORS            	SU0004496	S00015473	Sample type:Tissues | Species:Mus musculus | bleomycin/IPF:Y | saline/HLF:N | wt B6:Y	
SUBJECT_SAMPLE_FACTORS            	SU0004497	S00015474	Sample type:Tissues | Species:Mus musculus | bleomycin/IPF:Y | saline/HLF:N | wt B6:Y	
SUBJECT_SAMPLE_FACTORS            	SU0004498	S00015475	Sample type:Cells | Species:Homo sapiens | bleomycin/IPF:N | saline/HLF:Y | wt B6:N	
SUBJECT_SAMPLE_FACTORS            	SU0004499	S00015476	Sample type:Cells | Species:Homo sapiens | bleomycin/IPF:N | saline/HLF:Y | wt B6:N	
SUBJECT_SAMPLE_FACTORS            	SU0004500	S00015477	Sample type:Cells | Species:Homo sapiens | bleomycin/IPF:N | saline/HLF:Y | wt B6:N	
SUBJECT_SAMPLE_FACTORS            	SU0004501	S00015478	Sample type:Cells | Species:Homo sapiens | bleomycin/IPF:N | saline/HLF:Y | wt B6:N	
SUBJECT_SAMPLE_FACTORS            	SU0004502	S00015479	Sample type:Cells | Species:Homo sapiens | bleomycin/IPF:N | saline/HLF:Y | wt B6:N	
SUBJECT_SAMPLE_FACTORS            	SU0004503	S00015480	Sample type:Cells | Species:Homo sapiens | bleomycin/IPF:Y | saline/HLF:N | wt B6:N	
SUBJECT_SAMPLE_FACTORS            	SU0004504	S00015481	Sample type:Cells | Species:Homo sapiens | bleomycin/IPF:Y | saline/HLF:N | wt B6:N	
SUBJECT_SAMPLE_FACTORS            	SU0004505	S00015482	Sample type:Cells | Species:Homo sapiens | bleomycin/IPF:Y | saline/HLF:N | wt B6:N	
SUBJECT_SAMPLE_FACTORS            	SU0004506	S00015483	Sample type:Cells | Species:Homo sapiens | bleomycin/IPF:Y | saline/HLF:N | wt B6:N	
SUBJECT_SAMPLE_FACTORS            	SU0004507	S00015484	Sample type:Cells | Species:Homo sapiens | bleomycin/IPF:Y | saline/HLF:N | wt B6:N	
#COLLECTION
CO:COLLECTION_SUMMARY             	-
#TREATMENT
TR:TREATMENT_SUMMARY              	-
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY             	-
SP:SAMPLEPREP_PROTOCOL_FILENAME   	A010 - Ceramides.pdf
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_TYPE            	Phenyl
CH:INSTRUMENT_NAME                	6490A QQQ LC/MS (Agilent)
CH:COLUMN_NAME                    	Pinnacle DB Biphenyl, 140A, 1.9mkm, 2.1mm X 50mm (Restek)
CH:METHODS_FILENAME               	QM033_2mmC18_Ceramide_MRM
#ANALYSIS
AN:ANALYSIS_TYPE                  	MRM
AN:INSTRUMENT_NAME                	6490A QQQ LC/MS (Agilent)
AN:ANALYSIS_PROTOCOL_FILE         	A010 - Ceramides.pdf
AN:ACQUISITION_PARAMETERS_FILE    	QM033_2mmC18_Ceramide_MRM
AN:PROCESSING_PARAMETERS_FILE     	-
#MS
MS:INSTRUMENT_TYPE                	QQQ
MS:MS_TYPE                        	ESI
MS:ION_MODE                       	POSITIVE
#MS_METABOLITE_DATA
MS_METABOLITE_DATA:UNITS	counts
MS_METABOLITE_DATA_START
Sample	S00015469	S00015470	S00015471	S00015472	S00015473	S00015474	S00015475	S00015476	S00015477	S00015478	S00015479	S00015480	S00015481	S00015482	S00015483	S00015484
Factors	Sample type:Tissues | Species:Mus musculus | bleomycin/IPF:N | saline/HLF:Y | wt B6:Y	Sample type:Tissues | Species:Mus musculus | bleomycin/IPF:N | saline/HLF:Y | wt B6:Y	Sample type:Tissues | Species:Mus musculus | bleomycin/IPF:Y | saline/HLF:N | wt B6:Y	Sample type:Tissues | Species:Mus musculus | bleomycin/IPF:Y | saline/HLF:N | wt B6:Y	Sample type:Tissues | Species:Mus musculus | bleomycin/IPF:Y | saline/HLF:N | wt B6:Y	Sample type:Tissues | Species:Mus musculus | bleomycin/IPF:Y | saline/HLF:N | wt B6:Y	Sample type:Cells | Species:Homo sapiens | bleomycin/IPF:N | saline/HLF:Y | wt B6:N	Sample type:Cells | Species:Homo sapiens | bleomycin/IPF:N | saline/HLF:Y | wt B6:N	Sample type:Cells | Species:Homo sapiens | bleomycin/IPF:N | saline/HLF:Y | wt B6:N	Sample type:Cells | Species:Homo sapiens | bleomycin/IPF:N | saline/HLF:Y | wt B6:N	Sample type:Cells | Species:Homo sapiens | bleomycin/IPF:N | saline/HLF:Y | wt B6:N	Sample type:Cells | Species:Homo sapiens | bleomycin/IPF:Y | saline/HLF:N | wt B6:N	Sample type:Cells | Species:Homo sapiens | bleomycin/IPF:Y | saline/HLF:N | wt B6:N	Sample type:Cells | Species:Homo sapiens | bleomycin/IPF:Y | saline/HLF:N | wt B6:N	Sample type:Cells | Species:Homo sapiens | bleomycin/IPF:Y | saline/HLF:N | wt B6:N	Sample type:Cells | Species:Homo sapiens | bleomycin/IPF:Y | saline/HLF:N | wt B6:N
C22 CER	5703936.582	5015879.938	5467201.523	6207717.35	6183899.084	6941223.742	1789750.106	2430036.041	2736578.926	1759028.005	2164439.651	1043406.914	835781.0316	1106883.454	1281598.917	1273825.67
C24:1 CER	22157790.11	20169886.19	28601422.45	38763327.08	33508656.7	27013966.14	4395266.064	5056058.144	5724159.406	3835527.436	4598070.547	3316148.601	2967606.983	4547323.576	4872113.069	4184632.31
MS_METABOLITE_DATA_END
#METABOLITES
METABOLITES_START
metabolite_name	moverz_quant	ri	ri_type	pubchem_id	inchi_key	kegg_id	other_id	other_id_type
C22 CER				5283567				
C24:1 CER				5283568				
METABOLITES_END
