#METABOLOMICS WORKBENCH TEXT OUTPUT STUDY_ID:	ANALYSIS_ID:	
VERSION             	1
CREATED_ON          	2017-08-01
#PROJECT
PR:PROJECT_TITLE    	Metabolic regulation by PARP-1 in myofibroblast differentiation and fibrosis
PR:PROJECT_TYPE     	MS analysis
PR:PROJECT_SUMMARY  	Analysis of wild type lung tissue after saline or bleomycin treatment
PR:INSTITUTE        	University of Michigan
PR:DEPARTMENT       	Deaprtment of Pathology
PR:LABORATORY       	Sem H. Phan
PR:LAST_NAME        	Hu
PR:FIRST_NAME       	Biao
PR:ADDRESS          	Ann Arbor, MI
PR:EMAIL            	biaohu@med.umich.edu
PR:PHONE            	734-7635731
PR:FUNDING_SOURCE   	
PR:PROJECT_COMMENTS 	
PR:PUBLICATIONS     	
#STUDY
ST:STUDY_TITLE      	Metabolic analysis of Parp1 ko/wt Saline & Bleo Mouse Lung Fiboblasts and Human IPF & Normal Lung Fiboblasts 3
ST:STUDY_TYPE       	MS analysis
ST:STUDY_SUMMARY    	Glycolysis/TCA/Nucleotide analysis (especially interested in alpha-ketoglutarate) and NAD+ and related metabolite analysis for all samples
ST:INSTITUTE        	University of Michigan
ST:DEPARTMENT       	Biomedical Research Core Facilities
ST:LABORATORY       	Metabolomics core
ST:LAST_NAME        	Kachman
ST:FIRST_NAME       	Maureen
ST:ADDRESS          	6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
ST:EMAIL            	mkachman@med.umich.edu
ST:PHONE            	(734) 232-8175
ST:SUBMIT_DATE      	08/08/2014 02:01:21 PM
ST:NUM_GROUPS       	6
ST:TOTAL_SUBJECTS   	16
ST:STUDY_COMMENTS   	
#SUBJECT
SU:SUBJECT_TYPE                   	HUMAN
SU:SUBJECT_SPECIES                	Homo sapiens
SU:TAXONOMY_ID                    	9606
#SUBJECT_SAMPLE_FACTORS:          	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data
SUBJECT_SAMPLE_FACTORS            	SU0004492	S00015469	Sample type:Tissues | Species:Mus musculus | bleomycin/IPF:N | saline/HLF:Y | wt B6:Y	
SUBJECT_SAMPLE_FACTORS            	SU0004493	S00015470	Sample type:Tissues | Species:Mus musculus | bleomycin/IPF:N | saline/HLF:Y | wt B6:Y	
SUBJECT_SAMPLE_FACTORS            	SU0004494	S00015471	Sample type:Tissues | Species:Mus musculus | bleomycin/IPF:Y | saline/HLF:N | wt B6:Y	
SUBJECT_SAMPLE_FACTORS            	SU0004495	S00015472	Sample type:Tissues | Species:Mus musculus | bleomycin/IPF:Y | saline/HLF:N | wt B6:Y	
SUBJECT_SAMPLE_FACTORS            	SU0004496	S00015473	Sample type:Tissues | Species:Mus musculus | bleomycin/IPF:Y | saline/HLF:N | wt B6:Y	
SUBJECT_SAMPLE_FACTORS            	SU0004497	S00015474	Sample type:Tissues | Species:Mus musculus | bleomycin/IPF:Y | saline/HLF:N | wt B6:Y	
SUBJECT_SAMPLE_FACTORS            	SU0004498	S00015475	Sample type:Cells | Species:Homo sapiens | bleomycin/IPF:N | saline/HLF:Y | wt B6:N	
SUBJECT_SAMPLE_FACTORS            	SU0004499	S00015476	Sample type:Cells | Species:Homo sapiens | bleomycin/IPF:N | saline/HLF:Y | wt B6:N	
SUBJECT_SAMPLE_FACTORS            	SU0004500	S00015477	Sample type:Cells | Species:Homo sapiens | bleomycin/IPF:N | saline/HLF:Y | wt B6:N	
SUBJECT_SAMPLE_FACTORS            	SU0004501	S00015478	Sample type:Cells | Species:Homo sapiens | bleomycin/IPF:N | saline/HLF:Y | wt B6:N	
SUBJECT_SAMPLE_FACTORS            	SU0004502	S00015479	Sample type:Cells | Species:Homo sapiens | bleomycin/IPF:N | saline/HLF:Y | wt B6:N	
SUBJECT_SAMPLE_FACTORS            	SU0004503	S00015480	Sample type:Cells | Species:Homo sapiens | bleomycin/IPF:Y | saline/HLF:N | wt B6:N	
SUBJECT_SAMPLE_FACTORS            	SU0004504	S00015481	Sample type:Cells | Species:Homo sapiens | bleomycin/IPF:Y | saline/HLF:N | wt B6:N	
SUBJECT_SAMPLE_FACTORS            	SU0004505	S00015482	Sample type:Cells | Species:Homo sapiens | bleomycin/IPF:Y | saline/HLF:N | wt B6:N	
SUBJECT_SAMPLE_FACTORS            	SU0004506	S00015483	Sample type:Cells | Species:Homo sapiens | bleomycin/IPF:Y | saline/HLF:N | wt B6:N	
SUBJECT_SAMPLE_FACTORS            	SU0004507	S00015484	Sample type:Cells | Species:Homo sapiens | bleomycin/IPF:Y | saline/HLF:N | wt B6:N	
#COLLECTION
CO:COLLECTION_SUMMARY             	-
#TREATMENT
TR:TREATMENT_SUMMARY              	-
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY             	-
SP:SAMPLEPREP_PROTOCOL_FILENAME   	A011 - Glycolysis-TCA-nucleotides.pdf
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_TYPE            	1,4-bis(dimethylsiloxy)phenylene dimethyl polysiloxane
CH:INSTRUMENT_NAME                	GC_7890N MS_5975 (Agilent)
CH:COLUMN_NAME                    	DB-5MS 30 m x 250 mkm x 0.25 mkm (Agilent Technologies)
CH:METHODS_FILENAME               	ALPHA KETO ACIDS-FULL.M
#ANALYSIS
AN:ANALYSIS_TYPE                  	MS
AN:INSTRUMENT_NAME                	GC_7890N MS_5975 (Agilent)
AN:ANALYSIS_PROTOCOL_FILE         	A011 - Glycolysis-TCA-nucleotides.pdf
AN:ACQUISITION_PARAMETERS_FILE    	ALPHA KETO ACIDS-FULL.M
AN:PROCESSING_PARAMETERS_FILE     	-
#MS
MS:INSTRUMENT_TYPE                	Q
MS:MS_TYPE                        	EI
MS:ION_MODE                       	POSITIVE
#MS_METABOLITE_DATA
MS_METABOLITE_DATA:UNITS	uM
MS_METABOLITE_DATA_START
Sample	S00015469	S00015470	S00015471	S00015472	S00015473	S00015474	S00015475	S00015476	S00015477	S00015478	S00015479	S00015480	S00015481	S00015483	S00015484
Factors	Sample type:Tissues | Species:Mus musculus | bleomycin/IPF:N | saline/HLF:Y | wt B6:Y	Sample type:Tissues | Species:Mus musculus | bleomycin/IPF:N | saline/HLF:Y | wt B6:Y	Sample type:Tissues | Species:Mus musculus | bleomycin/IPF:Y | saline/HLF:N | wt B6:Y	Sample type:Tissues | Species:Mus musculus | bleomycin/IPF:Y | saline/HLF:N | wt B6:Y	Sample type:Tissues | Species:Mus musculus | bleomycin/IPF:Y | saline/HLF:N | wt B6:Y	Sample type:Tissues | Species:Mus musculus | bleomycin/IPF:Y | saline/HLF:N | wt B6:Y	Sample type:Cells | Species:Homo sapiens | bleomycin/IPF:N | saline/HLF:Y | wt B6:N	Sample type:Cells | Species:Homo sapiens | bleomycin/IPF:N | saline/HLF:Y | wt B6:N	Sample type:Cells | Species:Homo sapiens | bleomycin/IPF:N | saline/HLF:Y | wt B6:N	Sample type:Cells | Species:Homo sapiens | bleomycin/IPF:N | saline/HLF:Y | wt B6:N	Sample type:Cells | Species:Homo sapiens | bleomycin/IPF:N | saline/HLF:Y | wt B6:N	Sample type:Cells | Species:Homo sapiens | bleomycin/IPF:Y | saline/HLF:N | wt B6:N	Sample type:Cells | Species:Homo sapiens | bleomycin/IPF:Y | saline/HLF:N | wt B6:N	Sample type:Cells | Species:Homo sapiens | bleomycin/IPF:Y | saline/HLF:N | wt B6:N	Sample type:Cells | Species:Homo sapiens | bleomycin/IPF:Y | saline/HLF:N | wt B6:N
PYRUVIC ACID	1.2120888	1.2122738	1.2166433	1.2240997	1.2343615	1.2364795	1.2239584	1.2458525	1.2510062	1.2534862	1.2427141	1.2304312	1.2203002	1.2220662	1.2503706
DL-ASPARTIC ACID	82.228826	83.121791	108.35697	208.59358	125.90848	193.98439	4.8687735	8.2218718	8.0346042	8.0066445	12.427014	6.3079602	4.6913311	3.7597118	10.857118
FUMARIC ACID	3.8291594	3.9963144	4.8130148	5.2062561	4.7341648	7.0042579	2.8785189	2.7818424	2.7896288	2.7916152	2.8256436	2.8390397	2.8076263	2.788486	3.1031532
2-KETOGLUTARIC ACID	.2250877	.2279765	.2287517	.2308893	.2359683	.2380787	.2405373	.2504593	.2586114	.2526327	.2507883	.2645688	.2453656	.2402555	.2662557
L-ALANINE	2.1491892	2.4596988	4.2169022	7.72233	4.4421291	7.3234857	.5943472	1.0897502	1.3225341	1.1751392	1.6511848	2.0681367	1.3205668	1.1158445	4.0713104
L-GLUTAMINE	6.8331443	6.4461493	19.418498	31.933477	13.350189	19.513302	11.692336	20.719397	21.157957	22.825003	29.682731	14.900903	10.147254	8.7444129	22.633999
DL-GLUTAMIC ACID	6.3028675	6.1040444	8.6936756	14.290988	8.9094007	14.378001	2.8702344	4.8957194	4.775305	4.8792137	6.8351299	3.9097877	2.9760384	2.4428808	6.6109082
LACTIC ACID			1.9040661	3.0101775	2.9742998	6.3374418		.3731999	1.055963	1.1604786	.9431695	1.5286465	.3026353	.1891682	4.6088339
OXALACETIC ACID	.1944054	.1944054	.1944054	.1944054	.1944054	.1944054	.1944054	.1944054	.1944054	.1944054	.1944054	.1944054	.1944054	.1944054	.1944054
MS_METABOLITE_DATA_END
#METABOLITES
METABOLITES_START
metabolite_name	moverz_quant	ri	ri_type	pubchem_id	inchi_key	kegg_id	other_id	other_id_type
PYRUVIC ACID				1060				
DL-ASPARTIC ACID				424				
FUMARIC ACID				444972				
2-KETOGLUTARIC ACID				51				
L-ALANINE				5950				
L-GLUTAMINE				5961				
DL-GLUTAMIC ACID				611				
LACTIC ACID				612				
OXALACETIC ACID				970				
METABOLITES_END
