#METABOLOMICS WORKBENCH TEXT OUTPUT STUDY_ID:	ANALYSIS_ID:	
VERSION             	1
CREATED_ON          	2017-08-02
#PROJECT
PR:PROJECT_TITLE    	Diabetic Heart
PR:PROJECT_TYPE     	MS analysis
PR:PROJECT_SUMMARY  	Diabetic heart adrenergic signaling and metabolism
PR:INSTITUTE        	Oklahoma Medical Research Foundation
PR:DEPARTMENT       	Aging & Metabolism
PR:LABORATORY       	Humphries Lab
PR:LAST_NAME        	Humphries
PR:FIRST_NAME       	Kenneth
PR:ADDRESS          	Oklahoma City, OK
PR:EMAIL            	Kenneth-Humphries@omrf.org
PR:PHONE            	405-271-7584
PR:FUNDING_SOURCE   	
PR:PROJECT_COMMENTS 	
PR:PUBLICATIONS     	
#STUDY
ST:STUDY_TITLE      	Diabetic heart adrenergic signaling and metabolism (STZ & Isoproterenol, High Fat)
ST:STUDY_TYPE       	MS analysis
ST:STUDY_SUMMARY    	We are examing the effect of both diabetes and adrenergic activation on the metabolomic profile. STZ treatment was initiated 5 months before hearts were harvested. High fat/low fat diets were given for 13 weeks. All hearts were excised in the morning. For the STZ/isoproterenol experiment, mouse anesthesia was initiated with 4% isoflurane, and maintained with 2% and a homeothermic controller. After 5 min of equilibration, control and fasted mice were injected intraperitoneally with either saline (S) or 25ug/kg isoproterenol (I). After an additional 5 min, the whole heart was quickly excised, washed with ice-cold distilled water, dried off with a chem whipe, and flash frozen in liquid nitrogen. Hearts were stored at -80, and shipped using dry ice. The hearts from the high fat diet experiement were prepared in the same way, but no injection was given and total anesthesia time was 5min instead of 10min.
ST:INSTITUTE        	University of Michigan
ST:DEPARTMENT       	Biomedical Research Core Facilities
ST:LABORATORY       	Metabolomics core
ST:LAST_NAME        	Kachman
ST:FIRST_NAME       	Maureen
ST:ADDRESS          	6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
ST:EMAIL            	mkachman@med.umich.edu
ST:PHONE            	(734) 232-8175
ST:SUBMIT_DATE      	05/03/2016 04:31:47 PM
ST:NUM_GROUPS       	7
ST:TOTAL_SUBJECTS   	29
ST:STUDY_COMMENTS   	
#SUBJECT
SU:SUBJECT_TYPE                   	MOUSE
SU:SUBJECT_SPECIES                	Mus musculus
SU:TAXONOMY_ID                    	10090
#SUBJECT_SAMPLE_FACTORS:          	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data
SUBJECT_SAMPLE_FACTORS            	SU0014674	S00022376	Treatment:Saline | Type:Control	
SUBJECT_SAMPLE_FACTORS            	SU0014675	S00022377	Treatment:Saline | Type:Control	
SUBJECT_SAMPLE_FACTORS            	SU0014676	S00022378	Treatment:Saline | Type:Control	
SUBJECT_SAMPLE_FACTORS            	SU0014677	S00022379	Treatment:Saline | Type:Control	
SUBJECT_SAMPLE_FACTORS            	SU0014678	S00022380	Treatment:Saline | Type:Control	
SUBJECT_SAMPLE_FACTORS            	SU0014679	S00022381	Treatment:Saline | Type:STZ diabetic	
SUBJECT_SAMPLE_FACTORS            	SU0014680	S00022382	Treatment:Saline | Type:STZ diabetic	
SUBJECT_SAMPLE_FACTORS            	SU0014681	S00022383	Treatment:Saline | Type:STZ diabetic	
SUBJECT_SAMPLE_FACTORS            	SU0014682	S00022384	Treatment:Saline | Type:STZ diabetic	
SUBJECT_SAMPLE_FACTORS            	SU0014683	S00022385	Treatment:Saline | Type:STZ diabetic	
SUBJECT_SAMPLE_FACTORS            	SU0014684	S00022386	Treatment:Isoproterenol | Type:Control	
SUBJECT_SAMPLE_FACTORS            	SU0014685	S00022387	Treatment:Isoproterenol | Type:Control	
SUBJECT_SAMPLE_FACTORS            	SU0014686	S00022388	Treatment:Isoproterenol | Type:Control	
SUBJECT_SAMPLE_FACTORS            	SU0014687	S00022389	Treatment:Isoproterenol | Type:Control	
SUBJECT_SAMPLE_FACTORS            	SU0014688	S00022390	Treatment:Isoproterenol | Type:STZ diabetic	
SUBJECT_SAMPLE_FACTORS            	SU0014689	S00022391	Treatment:Isoproterenol | Type:STZ diabetic	
SUBJECT_SAMPLE_FACTORS            	SU0014690	S00022392	Treatment:Isoproterenol | Type:STZ diabetic	
SUBJECT_SAMPLE_FACTORS            	SU0014691	S00022393	Treatment:Isoproterenol | Type:STZ diabetic	
SUBJECT_SAMPLE_FACTORS            	SU0014692	S00022394	Treatment:Isoproterenol | Type:STZ diabetic	
SUBJECT_SAMPLE_FACTORS            	SU0014693	S00022395	Treatment:none | Type:Low fat diet	
SUBJECT_SAMPLE_FACTORS            	SU0014694	S00022396	Treatment:none | Type:Low fat diet	
SUBJECT_SAMPLE_FACTORS            	SU0014695	S00022397	Treatment:none | Type:Low fat diet	
SUBJECT_SAMPLE_FACTORS            	SU0014696	S00022398	Treatment:none | Type:Low fat diet	
SUBJECT_SAMPLE_FACTORS            	SU0014697	S00022399	Treatment:none | Type:Low fat diet	
SUBJECT_SAMPLE_FACTORS            	SU0014698	S00022400	Treatment:none | Type:High fat diet	
SUBJECT_SAMPLE_FACTORS            	SU0014699	S00022401	Treatment:none | Type:High fat diet	
SUBJECT_SAMPLE_FACTORS            	SU0014700	S00022402	Treatment:none | Type:High fat diet	
SUBJECT_SAMPLE_FACTORS            	SU0014701	S00022403	Treatment:none | Type:High fat diet	
SUBJECT_SAMPLE_FACTORS            	SU0014702	S00022404	Treatment:none | Type:High fat diet	
#COLLECTION
CO:COLLECTION_SUMMARY             	-
#TREATMENT
TR:TREATMENT_SUMMARY              	-
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY             	-
SP:SAMPLEPREP_PROTOCOL_FILENAME   	A011 - Glycolysis-TCA-nucleotides.pdf
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_TYPE            	1,4-bis(dimethylsiloxy)phenylene dimethyl polysiloxane
CH:INSTRUMENT_NAME                	GC_7890N MS_5975 (Agilent)
CH:COLUMN_NAME                    	DB-5MS 30 m x 250 mkm x 0.25 mkm (Agilent Technologies)
CH:METHODS_FILENAME               	ALPHA KETO ACIDS-FULL.M
#ANALYSIS
AN:ANALYSIS_TYPE                  	MS
AN:INSTRUMENT_NAME                	GC_7890N MS_5975 (Agilent)
AN:ANALYSIS_PROTOCOL_FILE         	A011 - Glycolysis-TCA-nucleotides.pdf
AN:ACQUISITION_PARAMETERS_FILE    	ALPHA KETO ACIDS-FULL.M
AN:PROCESSING_PARAMETERS_FILE     	-
#MS
MS:INSTRUMENT_TYPE                	Q
MS:MS_TYPE                        	EI
MS:ION_MODE                       	POSITIVE
#MS_METABOLITE_DATA
MS_METABOLITE_DATA:UNITS	nmol/mg
MS_METABOLITE_DATA_START
Sample	S00022376	S00022377	S00022378	S00022379	S00022380	S00022381	S00022382	S00022383	S00022384	S00022385	S00022386	S00022387	S00022388	S00022389	S00022390	S00022391	S00022392	S00022393	S00022394	S00022395	S00022396	S00022397	S00022398	S00022399	S00022400	S00022401	S00022402	S00022403	S00022404
Factors	Treatment:Saline | Type:Control	Treatment:Saline | Type:Control	Treatment:Saline | Type:Control	Treatment:Saline | Type:Control	Treatment:Saline | Type:Control	Treatment:Saline | Type:STZ diabetic	Treatment:Saline | Type:STZ diabetic	Treatment:Saline | Type:STZ diabetic	Treatment:Saline | Type:STZ diabetic	Treatment:Saline | Type:STZ diabetic	Treatment:Isoproterenol | Type:Control	Treatment:Isoproterenol | Type:Control	Treatment:Isoproterenol | Type:Control	Treatment:Isoproterenol | Type:Control	Treatment:Isoproterenol | Type:STZ diabetic	Treatment:Isoproterenol | Type:STZ diabetic	Treatment:Isoproterenol | Type:STZ diabetic	Treatment:Isoproterenol | Type:STZ diabetic	Treatment:Isoproterenol | Type:STZ diabetic	Treatment:none | Type:Low fat diet	Treatment:none | Type:Low fat diet	Treatment:none | Type:Low fat diet	Treatment:none | Type:Low fat diet	Treatment:none | Type:Low fat diet	Treatment:none | Type:High fat diet	Treatment:none | Type:High fat diet	Treatment:none | Type:High fat diet	Treatment:none | Type:High fat diet	Treatment:none | Type:High fat diet
PYRUVIC ACID	.002690792	.002306379	.002399577	.002241308	.002073886	.00236583	.003178321	.002318982	.002672803	.002911504	.001839684	.002116572	.001888965	.002122809	.002448401	.002093815	.002622569	.002181108	.002878324	.001423068	.001812885	.001955237	.002398084	.002230441	.002897453	.003293048	.002876439	.003012973	.002457201
FUMARIC ACID	.008144423	.00592678	.007117805	.007297742	.006704043	.01205887	.01053015	.012073034	.01051359	.011093094	.009155805	.009055551	.012555645	.011036272	.017066227	.025268475	.023782575	.021773673	.018682154	.010813554	.009892856	.016325485	.013554695	.012155283	.008546986	.009133208	.008051552	.010220977	.014689737
LACTIC ACID	2.205714186	2.262276034	1.984009675	2.551883202	1.942897671	2.206353908	3.200220849	2.376348998	2.678360616	2.332013786	1.58746671	2.030229717	1.706405406	2.075274799	1.739396761	1.789420107	2.082853441	2.146282546	2.643452685	2.300591919	2.152605693	.667909297	2.581113608	3.122829957	4.270708918	4.565542431	4.113164025	3.534402309	2.963567661
MS_METABOLITE_DATA_END
#METABOLITES
METABOLITES_START
metabolite_name	moverz_quant	ri	ri_type	pubchem_id	inchi_key	kegg_id	other_id	other_id_type
PYRUVIC ACID				1060				
FUMARIC ACID				444972				
LACTIC ACID				612				
METABOLITES_END
