                       INSTRUMENT CONTROL PARAMETERS:    GCMS1
                       ---------------------------------------

   D:\MassHunter\GCMS\1\Coloff Lab Methods\Dallas_Polar Metabolites split_10_1.M
      Thu Jan 12 05:47:46 2023

Control Information
------- -----------

Sample Inlet             : GC
Injection Source         : GC ALS
Injection Location:  Front
Mass Spectrometer        : Enabled


 No Sample Prep method has been assigned to this method.


GC
GC Summary
Run Time                                     28 min
Post Run Time                                9 min

Oven
Equilibration Time                           0.25 min
Max Temperature                              325 °C
Maximum Temperature Override                 Disabled
Slow Fan                                     Disabled
Temperature
Setpoint                                     On
(Initial)                                    60 °C
Hold Time                                    1 min
Post Run                                     60 °C
Program
#1 Rate                                      10 °C/min
#1 Value                                     320 °C
#1 Hold Time                                 1 min



ALS
ALS Errors                                   Pause for user interaction
Front Injector
Syringe Size                                 10 μL
Injection Volume                             1 μL
Solvent A Washes (PreInj)                    2
Solvent A Washes (PostInj)                   2
Solvent A Volume                             4 μL
Solvent B Washes (PreInj)                    2
Solvent B Washes (PostInj)                   2
Solvent B Volume                             4 μL
Sample Washes                                1
Sample Wash Volume                           1 μL
Sample Pumps                                 4
Dwell Time (PreInj)                          0 min
Dwell Time (PostInj)                         0 min
Solvent Wash Draw Speed                      300 μL/min
Solvent Wash Dispense Speed                  3000 μL/min
Sample Wash Draw Speed                       300 μL/min
Sample Wash Dispense Speed                   3000 μL/min
Injection Dispense Speed                     6000 μL/min
Viscosity Delay                              0 sec
Sample Depth                                 Disabled
Injection Type                               Standard
L1 Airgap                                    0 μL
Solvent Wash Mode                            A, B

Sample Overlap
Mode                                         Sample overlap is not enabled


Front SS Inlet He
Mode                                         Split
Heater                                       On    280 °C
Pressure                                     On    13.153 psi
Total Flow                                   On    19.229 mL/min
Septum Purge Flow                            On    3 mL/min
Gas Saver                                    On    20 After 3 min mL/min
Split Ratio                                  10 :1
Split Flow                                   14.754 mL/min

Thermal Aux 2 (MSD Transfer Line)
Temperature
Setpoint                                     On
(Initial)                                    280 °C
Post Run                                     0 °C


Column
Column Outlet Pressure                       0 psi
Column #1
Column Information                           Agilent 19091S-433: 93.92873
HP-5MS  5% Phenyl Methyl Silox               
Temperature Range                            0 °C—325 °C (325 °C)
Dimensions                                   30 m x 250 μm x 0.25 μm
Column lock                                  Unlocked
In                                           Front SS Inlet He
Out                                          MSD 
(Initial)                                    60 °C
Pressure                                     13.153 psi
Flow                                         1.4754 mL/min
Average Velocity                             44.484 cm/sec
Holdup Time                                  1.124 min
Flow
Setpoint                                     On
(Initial)                                    1.4754 mL/min
Post Run                                     0.57353 mL/min



Signals
Signal #1:  Test Plot
Description                                  Test Plot
Details                                      
Save                                         Off
Data Rate                                    50 Hz

Signal #2:  Test Plot
Description                                  Test Plot
Details                                      
Save                                         Off
Data Rate                                    50 Hz

Signal #3:  Test Plot
Description                                  Test Plot
Details                                      
Save                                         Off
Data Rate                                    50 Hz

Signal #4:  Test Plot
Description                                  Test Plot
Details                                      
Save                                         Off
Data Rate                                    50 Hz




MS Information
-- -----------


General Information
------- -----------


Acquisition Mode         : Scan
Solvent Delay (minutes)  : 7.6
Tune file                : D:\MassHunter\GCMS\1\5977\atune.u
EM Setting mode Gain     : 1.000000

Normal or Fast Scanning  : Normal Scanning
Trace Ion Detection      : Off
Run Time (if MS only)    : 10 minutes 

[Scan Parameters]
Start Time               : 7.6
Low Mass                 : 75
High Mass                : 1000
Threshold                : 100
A/D Samples:             : 4


[MSZones]

MS Source                   : 230 C   maximum 250 C
MS Quad                     : 150 C   maximum 200 C

Timed Events
----- ------
Number Events= 2


[Timed MS Detector Table Entries]

Time (min)                 State (MS On/off)
16.35                  off
16.65                  on





                     END OF MS ACQUISTION PARAMETERS


                        TUNE PARAMETERS for SN: US1840M009
                        ---------------------------------

 Trace Ion Detection is OFF.

    34.593   :   EMISSION     
    70.007   :   ENERGY       
    23.345   :   REPELLER     
    90.331   :   IONFOCUS     
    25.226   :   ENTRANCE_LENS
  1045.596   :   EMVOLTS      
                              1072.7  : Actual EMV 
                               1.00   : GAIN FACTOR 
  2621.000   :   AMUGAIN      
   136.938   :   AMUOFFSET    
     1.000   :   FILAMENT     
     1.000   :   DCPOLARITY   
     9.783   :   ENTLENSOFFSET
     0.000   :   Ion_Body     
     0.000   :   EXTLENS      
    64.000   :  MASSGAIN        
   -20.000   :  MASSOFFSET      

                        END OF TUNE PARAMETERS
                        ----------------------



                        END OF INSTRUMENT CONTROL PARAMETERS
                        ------------------------------------
