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MB Sample ID: SA001191
| Local Sample ID: | Blank |
| Subject ID: | SU000036 |
| Subject Type: | Animal |
| Subject Species: | Caenorhabditis elegans |
| Taxonomy ID: | 6239 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000036 |
| Subject Type: | Animal |
| Subject Species: | Caenorhabditis elegans |
| Taxonomy ID: | 6239 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| Blank | SA001191 | FL000421 | none | %13C |
Collection:
| Collection ID: | CO000019 |
| Collection Summary: | none |
| Sample Type: | Worms |
Treatment:
| Treatment ID: | TR001190 |
| Treatment Summary: | none |
Sample Preparation:
| Sampleprep ID: | SP000032 |
| Sampleprep Summary: | - |
| Sampleprep Protocol Filename: | IROAGlucoseNMR_Feb11_2014.txt |
| Sampleprep Protocol Comments: | 5%: 10.7 mg dissolved in 594 L of 99% D2O and 6 L of 111.11 mM d4-TSP in D2O 95%: 2.8 mg dissolved in 594 L of 99% D2O and 6 L of 111.11 mM d4-TSP in D2O Blank: 594 L of 99% D2O and 6 L of 111.11 mM d4-TSP in D2O |
Chromatography:
| Chromatography ID: | CH000020 |
| Chromatography Comments: | 100% solvent A for 1 min followed by a linear gradient to 20% B in 6 min, a linear gradient to 60% B in 2 min, a linear gradient to 95% B in 4 min, held for 2 min, and a 3.5 min return to the starting composition |
| Instrument Name: | Thermo Accela 1250 |
| Column Name: | Thermo Scientific Gold aQ |
| Column Temperature: | 40C |
| Flow Rate: | 600 uL/min |
| Sample Injection: | 3 uL |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile; 0.1% formic acid |
| Analytical Time: | 18.5 min |
| Chromatography Type: | Reversed phase |
Analysis:
| MB Sample ID: | SA001191 |
| Analysis ID: | AN000038 |
| Analysis Type: | MS |
| Instrument Name: | Thermo Scientific Q-Exactive |
| Software Version: | Thermo Xcalibur Qual Browser 2.2 |
| Chromatography ID: | CH000020 |
| Num Factors: | 3 |
| MB Sample ID: | SA001191 |
| Analysis ID: | AN000037 |
| Analysis Type: | NMR |
| Acquisition Parameters File: | NMR_Methods.docx |
| Processing Parameters File: | NMR_Methods.docx |
| Chromatography ID: | CH000020 |
| Num Factors: | 3 |
NMR:
| NMR ID: | NM000004 |
| Analysis ID: | AN000037 |
| Instrument Name: | Bruker Avance III |
| Instrument Type: | FT-NMR |
| NMR Experiment Type: | 1D 1H |
| Standard Concentration: | 10 mM |
| Spectrometer Frequency: | 500 MHz |
| NMR Probe: | 5mm FBBO-Z Probe |
| NMR Solvent: | D2O |
| NMR Tube Size: | 5mm |
| Pulse Sequence: | zg |
| Pulse Width: | 4 |
| Receiver Gain: | 152 |
| Chemical Shift Ref Cpd: | TSP |
| Temperature: | 27 |
| Number Of Scans: | 16 |
| Acquisition Time: | 4 s |
| Relaxation Delay: | 3 s |
| Spectral Width: | 5498.5 |
| Num Data Points Acquired: | 65536 |
| Real Data Points: | 16384 |
| Line Broadening: | 0.33 Hz |
| Zero Filling: | Yes |
| Apodization: | Exponential |
| Baseline Correction Method: | Whittaker Smoother |
| Chemical Shift Ref Std: | TSP |
