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MB Sample ID: SA002140
Local Sample ID: | Pool 3c |
Subject ID: | SU000066 |
Subject Type: | Animal |
Subject Species: | Mirounga angustirostris |
Taxonomy ID: | 9716 |
Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU000066 |
Subject Type: | Animal |
Subject Species: | Mirounga angustirostris |
Taxonomy ID: | 9716 |
Species Group: | Mammal |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
Pool 3c | SA002140 | FL000654 | Pool3 | Treatment |
Collection:
Collection ID: | CO000049 |
Collection Summary: | - |
Sample Type: | Blood |
Treatment:
Treatment ID: | TR000067 |
Sample Preparation:
Sampleprep ID: | SP000062 |
Sampleprep Summary: | - |
Sampleprep Protocol Filename: | SOP_Extraction_of_Blood_Samples.pdf SOP_Derivatization_of_Samples-Standards_2.pdf |
Processing Method: | Homogenization, protein precipitation via extraction, Solvent Removal w/ Speed Vac |
Processing Storage Conditions: | On Ice |
Extraction Method: | 3:3:2 Acetonitrile/Isopropanol/Water |
Extract Concentration Dilution: | 30uL of plasma/ml |
Extract Cleanup: | 1:1 Acetonitrile/Water |
Extract Storage: | -20C Freezer |
Sample Derivatization: | 10ul of 40mg/ml MeOX in pyridine, 91ul of MSTFA +FAMEs mixture |
Sample Spiking: | FAMEs |
Combined analysis:
Analysis ID | AN000084 |
---|---|
Analysis type | MS |
Chromatography type | GC |
Chromatography system | Agilent 6890N |
Column | Restek corporation Rtx-5Sil MS |
MS Type | EI |
MS instrument type | GC-TOF |
MS instrument name | Leco Pegasus III GC TOF |
Ion Mode | POSITIVE |
Units | Peak Area |
Chromatography:
Chromatography ID: | CH000053 |
Methods Filename: | SOP_GCTOF11082012.pdf |
Instrument Name: | Agilent 6890N |
Column Name: | Restek corporation Rtx-5Sil MS |
Column Temperature: | 50-330oC |
Flow Rate: | 1ml/min |
Injection Temperature: | 50C ramped to 250C by 12C s-1 |
Sample Injection: | 0.5uL |
Oven Temperature: | 50C for 1 min, then ramped at 20C min-1 to 330C, held constant for 5 min |
Transferline Temperature: | 230C |
Washing Buffer: | Ethyl Acetate |
Sample Loop Size: | 30 m length x 0.25 mm internal diameter |
Randomization Order: | Excel generated |
Chromatography Type: | GC |
MS:
MS ID: | MS000065 |
Analysis ID: | AN000084 |
Instrument Name: | Leco Pegasus III GC TOF |
Instrument Type: | GC-TOF |
MS Type: | EI |
Ion Mode: | POSITIVE |
Ion Source Temperature: | 250°C |
Ionization Energy: | -70eV |
Mass Accuracy: | Nominal |
Source Temperature: | 250°C |
Scanning Range: | 80-500 Da |
Acquisition Parameters File: | SOP_GCTOF11082012.pdf |
Processing Parameters File: | Data_Dictionary_Fiehn_Laboratory_GCTOF_MS_primary_metabolism_09-27-2013_general.pdf |