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MB Sample ID: SA003868
Local Sample ID: | F_HFAT_FAC681 |
Subject ID: | SU000093 |
Subject Type: | Animal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | BXD genotype panel |
Age Or Age Range: | 201-740 days |
Weight Or Weight Range: | 19.67-76.2g |
Gender: | Female |
Animal Animal Supplier: | The Jackson Laboratory |
Animal Feed: | Harlan Teklad 2018 (control) and 06414 (high fat) |
Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU000093 |
Subject Type: | Animal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | BXD genotype panel |
Age Or Age Range: | 201-740 days |
Weight Or Weight Range: | 19.67-76.2g |
Gender: | Female |
Animal Animal Supplier: | The Jackson Laboratory |
Animal Feed: | Harlan Teklad 2018 (control) and 06414 (high fat) |
Species Group: | Mammal |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
F_HFAT_FAC681 | SA003868 | FL001007 | BXD90 | genotype |
F_HFAT_FAC681 | SA003868 | FL001007 | High-Fat | Diet |
Collection:
Collection ID: | CO000076 |
Collection Summary: | - |
Sample Type: | Feces |
Treatment:
Treatment ID: | TR000094 |
Sample Preparation:
Sampleprep ID: | SP000089 |
Sampleprep Summary: | Samples were extracted using 95:5 water:methanol. |
Sampleprep Protocol Filename: | RTI_WILLIAMS_Metabolomics_Procedure.docx |
Extraction Method: | 95:5 water:methanol |
Extract Storage: | -80 C |
Sample Spiking: | L-Tryptophan-d5 |
Organ: | Feces |
Combined analysis:
Analysis ID | AN000117 | AN000118 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | Reversed phase |
Chromatography system | Waters Acquity | Waters Acquity |
Column | Waters Acquity HSS T3 (100 x 2.1mm,1.8um) | Waters Acquity HSS T3 (100 x 2.1mm,1.8um) |
MS Type | ESI | ESI |
MS instrument type | QTOF | QTOF |
MS instrument name | Waters Synapt G2 QTOF | Waters Synapt G2 QTOF |
Ion Mode | POSITIVE | NEGATIVE |
Units | Normalized abundance | Normalized abundance |
Chromatography:
Chromatography ID: | CH000080 |
Instrument Name: | Waters Acquity |
Column Name: | Waters Acquity HSS T3 (100 x 2.1mm,1.8um) |
Column Pressure: | 6000-10000 |
Column Temperature: | 50 C |
Flow Gradient: | Time(min) Flow Rate %A %B Curve ; 1. Initial 0.400 100.0 0.0 ; 2. 1.00 0.400 100.0 0.0 6 ; 3. 16.00 0.400 0.0 100.0 6 ; 4. 20.00 0.400 0.0 100.0 6 ; 5. 22.00 0.400 100.0 0.0 6 |
Flow Rate: | 0.4 mL/min |
Injection Temperature: | 8 C |
Internal Standard: | L-Tryptophan-d5 |
Solvent A: | 100% water; 0.1% formic acid |
Solvent B: | 100% methanol; 0.1% formic acid |
Analytical Time: | 22 min |
Weak Wash Solvent Name: | 5%MeOH |
Weak Wash Volume: | 1000 uL |
Strong Wash Solvent Name: | 80%MeOH |
Strong Wash Volume: | 1000 uL |
Target Sample Temperature: | 8 C |
Sample Loop Size: | 10 uL |
Sample Syringe Size: | 100 uL |
Randomization Order: | Yes |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS000093 |
Analysis ID: | AN000117 |
Instrument Name: | Waters Synapt G2 QTOF |
Instrument Type: | QTOF |
MS Type: | ESI |
Ion Mode: | POSITIVE |
Capillary Temperature: | 110 C |
Capillary Voltage: | 3.2 kV |
Collision Energy: | 4 |
Helium Flow: | 180 |
Ionization: | ES+ |
Mass Accuracy: | 10 ppm |
Source Temperature: | 110 C |
Dataformat: | Continuum |
Desolvation Gas Flow: | 400 L/Hr |
Desolvation Temperature: | 400 C |
Resolution Setting: | 18000 |
Scan Range Moverz: | 50-1000 m/s |
Scanning Cycle: | 1 s |
Tube Lens Voltage: | 75 |
MS ID: | MS000094 |
Analysis ID: | AN000118 |
Instrument Name: | Waters Synapt G2 QTOF |
Instrument Type: | QTOF |
MS Type: | ESI |
Ion Mode: | NEGATIVE |
Capillary Temperature: | 110 C |
Capillary Voltage: | 2.8 kV |
Collision Energy: | 4 |
Helium Flow: | 180 |
Ionization: | ES- |
Source Temperature: | 110 C |
Dataformat: | Continuum |
Desolvation Gas Flow: | 400 L/Hr |
Desolvation Temperature: | 400 C |
Resolution Setting: | 18000 |
Scan Range Moverz: | 50-1000 m/z |
Scanning Cycle: | 1 s |
Tube Lens Voltage: | 74 |