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MB Sample ID: SA006059
Local Sample ID: | tet-normox-13C/15N-Gln-plate-1 |
Subject ID: | SU000129 |
Subject Type: | Human cells |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Genotype Strain: | P493 |
Species Group: | Human |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU000129 |
Subject Type: | Human cells |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Genotype Strain: | P493 |
Species Group: | Human |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
tet-normox-13C/15N-Gln-plate-1 | SA006059 | FL001564 | normoxia | Oxygen Condition |
tet-normox-13C/15N-Gln-plate-1 | SA006059 | FL001564 | Tetracycline | Treatment |
Collection:
Collection ID: | CO000113 |
Collection Summary: | - |
Collection Protocol Comments: | Collection and quench of cells in tissue culture |
Sample Type: | B Cells |
Treatment:
Treatment ID: | TR000131 |
Treatment Protocol Comments: | hypox-13C/15N-Gln: cell culture under hypoxia for 24hr + 24hr with [U-13C/15N]-Gln; tet-hypox-13C/15N-Gln: cell culture under hypoxia with 1ug/ml Tetracycline for 24hr + 24hr with [U-13C/15N]-Gln; tet-normox-13C/15N-Gln: cell culture under normoxia with 1ug/ml Tetracycline for 24hr + 24hr with [U-13C/15N]-Gln; normox-13C/15N-Gln: cell culture under normoxia for 24hr + 24hr with [U-13C/15N]-Gln |
Sample Preparation:
Sampleprep ID: | SP000126 |
Sampleprep Summary: | - |
Sampleprep Protocol Comments: | Protein extraction and quantification. |Preparation of polar samples for GCMS and NMR. | Lipid extraction and preparation for FTMS. |
Combined analysis:
Analysis ID | AN000183 | AN000184 | AN000185 |
---|---|---|---|
Analysis type | MS | MS | MS |
Chromatography type | GC | GC | GC |
Chromatography system | |||
Column | |||
MS Type | ESI | ESI | EI |
MS instrument type | Single quadrupole | Single quadrupole | GC-ITQ |
MS instrument name | Thermo LTQ-FT | Thermo LTQ-FT | Thermo ITQ |
Ion Mode | POSITIVE | NEGATIVE | POSITIVE |
Units | Peak area | Peak area | Peak area |
Chromatography:
Chromatography ID: | CH000124 |
Chromatography Type: | GC |
MS:
MS ID: | MS000146 |
Analysis ID: | AN000183 |
Instrument Name: | Thermo LTQ-FT |
Instrument Type: | Single quadrupole |
MS Type: | ESI |
Ion Mode: | POSITIVE |
MS ID: | MS000147 |
Analysis ID: | AN000184 |
Instrument Name: | Thermo LTQ-FT |
Instrument Type: | Single quadrupole |
MS Type: | ESI |
Ion Mode: | NEGATIVE |
MS ID: | MS000148 |
Analysis ID: | AN000185 |
Instrument Name: | Thermo ITQ |
Instrument Type: | GC-ITQ |
MS Type: | EI |
Ion Mode: | POSITIVE |