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MB Sample ID: SA007457
Local Sample ID: | SRM 1950_A |
Subject ID: | SU000155 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Species Group: | Human |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU000155 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Species Group: | Human |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
SRM 1950_A | SA007457 | FL001885 | QC | Diet |
Collection:
Collection ID: | CO000139 |
Collection Summary: | - |
Collection Protocol ID: | SOP_LE_Matyash |
Collection Protocol Filename: | SOP_LE_02_Matyash_RP_NIST.pdf |
Sample Type: | blood |
Storage Conditions: | -80C |
Treatment:
Treatment ID: | TR000157 |
Sample Preparation:
Sampleprep ID: | SP000152 |
Sampleprep Summary: | MTBE lipid extraction |
Sampleprep Protocol ID: | SOP_LE_Matyash |
Sampleprep Protocol Filename: | SOP_LE_02_Matyash_RP_NIST.pdf |
Processing Storage Conditions: | On ice |
Extraction Method: | MTBE |
Extract Concentration Dilution: | 40 uLplasma/40uL reconstitution solvent |
Sample Resuspension: | 40 uL isopropanol |
Sample Spiking: | Internal standards listed in protocol |
Organ: | blodo plasma |
Combined analysis:
Analysis ID | AN000218 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Thermo Dionex Ultimate 3000 |
Column | Waters Acquity BEH C18 (50 x 2.1mm,1.7um) |
MS Type | ESI |
MS instrument type | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap |
Ion Mode | POSITIVE |
Units | nmol/mL serum |
Chromatography:
Chromatography ID: | CH000150 |
Chromatography Summary: | Reverse phase |
Methods Filename: | m_lipids_AIF_pos |
Instrument Name: | Thermo Dionex Ultimate 3000 |
Column Name: | Waters Acquity BEH C18 (50 x 2.1mm,1.7um) |
Column Pressure: | 800 bar (max) |
Column Temperature: | 35 |
Flow Gradient: | none |
Flow Rate: | 0.5 mL/min |
Injection Temperature: | 4 C |
Sample Injection: | 3 uL for pos mode, 10 uL for neg mode |
Solvent A: | 40% water/60% acetonitrile; 0.1% formic acid; 10 mM ammonium formate |
Solvent B: | 90% isopropanol/8% acetonitrile/2% water; 0.1% formic acid; 10 mM ammonium formate |
Analytical Time: | 20 min |
Capillary Voltage: | 50 C |
Running Voltage: | 3.5 kV |
Randomization Order: | 3b,1b,1c,3c,2c,2a,2b,1a,3a |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS000170 |
Analysis ID: | AN000218 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
Ion Mode: | POSITIVE |
Capillary Temperature: | 300 (+), 250 (-) |
Collision Energy: | 25 NCE |
Dry Gas Flow: | 30 (+), 25 (-) |
Ion Spray Voltage: | 3.5 kV |
Mass Accuracy: | < 2ppm |
Precursor Type: | Dependent upon class |
Spray Voltage: | 3.5 kV |
Dataformat: | .RAW |
Scan Range Moverz: | 120-2000 |
Skimmer Voltage: | 15 |
Acquisition Parameters File: | QE1_REP_34_sequence |